Fluorescence immunochromatography quantitative detector

Abstract

The utility model discloses a fluorescence immunochromatography quantitative detector, which relates to the technical field of fluorescence immunoassay analyzers, and particularly comprises a detector, and a bracket for placing a reagent strip is arranged in a detection cavity; a top cover is detachably mounted at the top of the detection cavity, a third electric actuator is mounted at the top in the top cover, and a fluorescence receiving part is arranged at the lower end of the third electric actuator; an adjusting motor is arranged on one side of the mounting frame body, a rotating shaft of the adjusting motor is connected with the mounting seat body, a second electric actuator is arranged in the mounting seat body, a light source is mounted at the bottom of the telescopic end of the second electric actuator, and light beams emitted by the light source face the reagent strip and are received by the fluorescence receiving part after being reflected by the reagent strip. During use, the distance between the light source and the reagent strip is conveniently adjusted, light rays are concentrated on the reagent strip to the greatest extent, the third electric actuator can be controlled to adjust the distance between the fluorescence receiving part and the reagent strip, and the fluorescence receiving part can receive light signals at the optimal distance.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence immunoassay analyzer technology, specifically a fluorescence immunochromatographic quantitative detection instrument. Background Technology

[0002] In a fluorescence immunoassay analyzer, the antigens in the sample are first bound to fluorescently labeled antibodies on a labeling pad using a processing reagent, forming an immune complex. This complex diffuses along the nitrocellulose membrane under chromatographic action to the detection area, which is coated with another monoclonal antibody, thus forming a double-antibody sandwich complex. The free fluorescent antibody remains attached to the control area. When the test card (reagent strip) is inserted into the analyzer, a light source illuminates the card. The light source is controlled by a module, and the analyzer receives and analyzes the fluorescence intensity from both the detection and control areas.

[0003] The internal optical path of a fluorescence immunoassay analyzer is generally either a confocal or non-confocal optical path. In a non-confocal fluorescence immunoassay analyzer, the photodetector (photomultiplier tube) receives the light signal and converts the fluorescence intensity into an electrical signal. The analyzer then performs calculations and quantitative analysis to derive the structure. However, in existing non-confocal fluorescence immunoassay analyzers, the positions of the internal light source and photodetector are fixed, and the distance between them and the detection card cannot be adjusted. This leads to calibration failure when there is a deviation between the light emitted by the light source and the detection card. Utility Model Content

[0004] This invention provides a fluorescence immunochromatographic quantitative detection instrument, which has the advantage of adjusting the distance between the light source and the reagent strip so that the light emitted by the light source is focused on the reagent strip, thus solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A fluorescence immunochromatographic quantitative detection instrument was designed, including an instrument, a detection chamber on the top of the instrument, and a support for placing reagent strips inside the detection chamber;

[0007] The top of the detection chamber is detachably fitted with a top cover, and a third electric actuator is installed inside the top of the top cover. A fluorescent receiving part is provided at the lower end of the third electric actuator.

[0008] The third electric actuator has at least one mounting bracket on its side. An adjustment motor is provided on one side of the mounting bracket. The rotating shaft of the adjustment motor is connected to the mounting base. The mounting base contains a second electric actuator. A light source is installed at the bottom of the telescopic end of the second electric actuator. The light beam emitted by the light source is directed toward the reagent strip and is received by the fluorescence receiving unit after being reflected by the reagent strip.

[0009] Preferably, the detector includes a housing, with a display screen and operation buttons on the top of the housing, and a control panel inside the housing;

[0010] The light source, the third electric actuator, the regulating motor, the second electric actuator, the fluorescent receiver, the display screen, and the operation buttons are all electrically connected to the control board.

[0011] Preferably, the detection chamber is located at the top of the housing, and one end of the detection chamber extends through the side of the housing;

[0012] The support for holding the reagent strips includes a horizontally arranged support frame, the two sides of which are connected to the inner wall of the detection chamber via guide rails.

[0013] A support plate is installed inside the support frame, and a placement plate is provided above the support plate. A placement groove is provided in the middle of the placement plate. The reagent strip is placed in the placement groove. When the support plate is placed inside the detection chamber, the placement groove and the reagent strip are located below the fluorescence receiving part.

[0014] Preferably, a first electric actuator is installed on the inner wall of the detection chamber. The telescopic end of the first electric actuator is connected to the support frame. The first electric actuator can drive the support frame to be pulled in and out of the detection chamber. The first electric actuator is electrically connected to the control board.

[0015] Preferably, each of the four corners of the placement board is vertically connected to a support column, and each support column is coaxially connected to a plug rod at its bottom.

[0016] The insertion rod and support are arranged in a stepped manner. The support plate has holes corresponding to the insertion rods, and the insertion rods are placed in the holes. The bottom of the support is placed on the support plate.

[0017] Preferably, a sealing plate is provided at the end of the support frame, and the sealing plate seals the opening of the detection chamber after the bearing plate is placed inside the detection chamber.

[0018] Preferably, the fluorescence receiver includes a housing, with a filter group at the bottom of the housing, and a lens group, an emission monochromator, a slit, and a photomultiplier tube sequentially installed inside the housing above the filter group.

[0019] Compared with the prior art, this utility model allows for easy adjustment of the distance between the light source and the reagent strip during use, so that the light is concentrated on the reagent strip to the greatest extent. At the same time, it can also control the third electric actuator to adjust the distance between the fluorescence receiving part and the reagent strip, so that the fluorescence receiving part can receive the light signal at the optimal distance.

[0020] Furthermore, during testing, the operator can remove the placement plate from the support plate, allowing the operator to place the test strip in the placement slot. Then, the placement plate is placed on top of the support plate and secured, ensuring that the test strip is placed in the placement slot without being obstructed by other objects. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the present utility model. Figure 1 .

[0022] Figure 2 This is a schematic diagram of the structure of the present utility model. Figure 2 .

[0023] Figure 3 This is a schematic diagram of the inside of the detection cavity in this utility model.

[0024] Figure 4 This is a cross-sectional view of the present invention.

[0025] Figure 5 This is a structural diagram of the fluorescent receiving part in this utility model.

[0026] In the diagram: 1. Outer shell; 2. Top cover; 3. Display screen; 4. Operation buttons; 5. Placement plate; 6. Placement slot; 7. Sealing plate; 8. Detection chamber; 9. Bearing plate; 10. Support frame; 11. Column; 12. First electric actuator; 13. Third electric actuator; 14. Fluorescent receiver; 15. Second electric actuator; 16. Mounting base; 17. Light source; 18. Control board; 19. Insert rod; 20. Adjustment motor; 21. Mounting frame; 22. Housing; 23. Photomultiplier tube; 24. Slit; 25. Emitting monochromator; 26. Lens group; 27. Filter group. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Please see Figures 1 to 5 This utility model provides a technical solution: a fluorescence immunochromatographic quantitative detector, including a detector, the detector including a shell 1, the bottom four corners of the shell 1 are respectively provided with support pads, the top of the shell 1 is provided with a display screen 3 and operation buttons 4, the shell 1 is provided with a control board 18, and the display screen 3 and operation buttons 4 are electrically connected to the control board 18 respectively.

[0029] The detector has a detection chamber 8 on its top, such as Figure 1 and Figure 2 As shown, the detection cavity 8 is located on the top of the housing 1. The detection cavity 8 is located on one side of the display screen 3 and the operation button 4, and the display screen 3 is tilted, which makes it convenient for the operator to read the information on the screen.

[0030] like Figure 1 and Figure 3 As shown, one end of the detection chamber 8 penetrates the side of the outer shell 1, and a bracket for placing the reagent strip is provided inside the detection chamber 8. Its specific structure includes a support frame 10 horizontally arranged in the detection chamber 8. The two sides of the support frame 10 are connected to the inner wall of the detection chamber 8 through guide rails, so that the support frame 10 can be pulled in or out by the operator like a drawer.

[0031] A support plate 9 is installed inside the support frame 10. A placement plate 5 is provided above the support plate 9. A support column 11 is vertically connected to the bottom of the four corners of the placement plate 5. A plug rod 19 is coaxially connected to the bottom of the support column 11. The plug rod 19 and the support column 11 are stepped. The support plate 9 is provided with holes corresponding to the plug rod 19. The plug rod 19 is placed in the holes. The bottom of the support column 11 is placed on the support plate 9.

[0032] The placement plate 5 has a placement groove 6 in the middle, and the reagent strip is placed in the placement groove 6. The placement plate 5 in this application is convenient for the operator to pick up. In the actual operation, the operator can pinch the placement plate 5 with his thumb and forefinger and take it off the support plate 9 to expose the placement plate 5 completely. In this way, the operator can place the reagent strip in the placement groove 6 and then place the placement plate 5 on top of the support plate 9 for fixation. The advantage of this is that the reagent strip will not be blocked by any object when placing it, which makes it convenient for the reagent strip to be placed in the placement groove 6.

[0033] Furthermore, such as Figure 3 As shown, a first electric actuator 12 is installed on the inner wall of the detection cavity 8. The telescopic end of the first electric actuator 12 is connected to the support frame 10. The first electric actuator 12 can drive the support frame 10 to be pulled in and out of the detection cavity 8. The first electric actuator 12 is electrically connected to the control board 18.

[0034] The first electric actuator 12 can drive the support frame 10 to extend out of the detection chamber 8, or drive the already pulled-out support frame 10 to retract back into the detection chamber 8. The first electric actuator 12 can slowly drive the support frame 10 to reciprocate, avoiding excessive speed when manually pushing the support frame 10.

[0035] The most important feature of this application is that a top cover 2 is detachably installed on the top of the detection chamber 8, and a third electric actuator 13 is installed inside the top of the top cover 2. A fluorescence receiving part 14 is provided at the lower end of the third electric actuator 13. It should be noted that when the support plate 9 is placed inside the detection chamber 8, the placement slot 6 and the reagent strip are located below the fluorescence receiving part 14. The distance between the fluorescence receiving part 14 and the reagent strip can be adjusted under the drive of the third electric actuator 13.

[0036] Meanwhile, at least one mounting bracket 21 is provided on the side of the third electric actuator 13. An adjusting motor 20 is provided on one side of the mounting bracket 21. The rotating shaft of the adjusting motor 20 is connected to the mounting base 16. A second electric actuator 15 is provided inside the mounting base 16. A light source 17 is installed at the bottom of the telescopic end of the second electric actuator 15. The light beam emitted by the light source 17 is directed towards the reagent strip and, after reflection by the reagent strip, is received by the fluorescence receiving unit 14. For example... Figure 4 As shown, the adjusting motor 20 can drive the second electric actuator 15 to rotate, thereby adjusting the angle between the second electric actuator 15 and the placement plate 5, so as to facilitate the adjustment of the position of the light source 17 shining on the reagent strip. At the same time, the second electric actuator 15 can also drive the light source 17 to move towards the reagent strip, so that the light emitted by the light source is focused on the reagent strip.

[0037] Since there is more than one mounting bracket 21, there is also more than one light source 17. In actual use, there are at least two light sources 17 to increase the intensity of the light reflected from the reagent strip. The light source 17, the third electric actuator 13, the adjusting motor 20, the second electric actuator 15, and the fluorescence receiver 14 are all electrically connected to the control board 18. Operators can control them individually via the operation buttons 4. This allows for easy adjustment of the distance between the light source 17 and the reagent strip, maximizing the concentration of light on the reagent strip. It also allows control of the third electric actuator 13 to adjust the distance between the fluorescence receiver 14 and the reagent strip, ensuring the fluorescence receiver 14 receives the light signal at the optimal distance.

[0038] Furthermore, since the opening of detection cavity 8 is open, in order to avoid interference from external light sources, such as... Figure 1 As shown, a sealing plate 7 is provided at the end of the support frame 10. When the bearing plate 9 is placed inside the detection cavity 8, the sealing plate 7 seals the opening of the detection cavity 8.

[0039] This application also proposes a specific structure for the fluorescence receiver 14. The fluorescence receiver 14 includes a housing 22. The top of the housing 22 is connected to the lower end of the third electric actuator 13. A filter group 27 is provided at the bottom of the housing 22. A lens group 26, an emission monochromator 25, a slit 24 (e.g., an aperture), and a photomultiplier tube 23 are sequentially installed inside the housing 22 above the filter group 27. The working process is as follows: the light emitted by the light source 17 shines on the reagent strip. The light reflected by the reagent strip reaches the bottom of the fluorescence receiver 14. After being filtered by the filter group 27, the light is focused on the emission monochromator 25 by the lens group 26. Then, the light beam emitted by the emission monochromator 25 is captured by the photomultiplier tube 23 inside the slit 24. The photomultiplier tube 23 transmits the captured signal to the control board 18. After being analyzed by the control board 18, the signal is displayed on the display screen 3.

[0040] It should also be noted that the fluorescent receiving unit 14 is installed inside the top cover 2. The top cover and the outer shell are fastened together by a latch or bolt, which makes the top cover 2 easy to open. At the same time, the inside of the detector can be cleaned after the top cover 2 is opened.

[0041] In summary, the electrical connections in this application are made by means of wires, signal lines, and control lines, thereby enabling the transmission of electrical energy and signals.

[0042] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model; the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In addition, unless otherwise explicitly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances. Moreover, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0043] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fluorescent immuno-chromatographic quantitative detection instrument, comprising a detection instrument, characterized in that, The top of the detector is provided with a detection cavity (8), and the detection cavity (8) is provided with a support for placing a reagent strip; The top of the detection cavity (8) is detachably provided with a top cover (2), and the top cover (2) is provided with a third electric actuator (13) at the top. The side of the third electric actuator (13) is provided with at least one mounting bracket (21), and the mounting bracket (21) is provided with an adjusting motor (20) on one side.

2. The fluorescent immuno-chromatographic quantitative detector according to claim 1, characterized in that, The adjusting motor (20) is connected with a mounting seat (16), and the mounting seat (16) is provided with a second electric actuator (15). The bottom of the second electric actuator (15) is provided with a light source (17), and the light beam emitted by the light source (17) is directed to the reagent strip and is received by the fluorescence receiving part (14) after being reflected by the reagent strip. 3.The fluorescent immuno-chromatographic quantitative detector according to claim 2, characterized in that, The detector comprises a shell (1), and the top of the shell (1) is provided with a display screen (3) and an operation button (4). The light source (17), the third electric actuator (13), the adjusting motor (20), the second electric actuator (15), the fluorescence receiving part (14), the display screen (3) and the operation button (4) are electrically connected with the control panel (18). The detection cavity (8) is arranged at the top of the shell (1), and one end of the detection cavity (8) penetrates through the side of the shell (1).

4. The fluorescent immuno-chromatographic quantitative detector according to claim 3, characterized in that, The support for placing the reagent strip comprises a horizontally arranged support frame (10), and the two sides of the support frame (10) are connected with the inner wall of the detection cavity (8) through guide rails.

5. The fluorescent immuno-chromatographic quantitative detector according to claim 4, characterized in that, A bearing plate (9) is arranged in the support frame (10), and the bearing plate (9) is provided with a placing plate (5) above. The middle of the placing plate (5) is provided with a placing groove (6), and the reagent strip is placed in the placing groove (6).

6. The fluorescent immuno-chromatographic quantitative detector according to claim 5, characterized in that, When the bearing plate (9) is placed in the detection cavity (8), the placing groove (6) and the reagent strip are located below the fluorescence receiving part (14).

7. The fluorescent immuno-chromatographic quantitative detector according to any one of claims 1-6, characterized in that, A first electric actuator (12) is arranged on the inner wall of the detection cavity (8), and the telescopic end of the first electric actuator (12) is connected with the support frame (10). The four corners of the placing plate (5) are respectively connected with support columns (11) perpendicularly. The bottom of the support column (11) is coaxially connected with a plug rod (19). The plug rod (19) and the support column (11) are arranged in steps, and the bearing plate (9) is respectively provided with holes corresponding to the plug rods (19). The plug rods (19) are respectively arranged in the holes, and the bottom of the support column (11) is arranged on the bearing plate (9). The end of the support frame (10) is provided with a sealing plate (7), and when the bearing plate (9) is placed in the detection cavity (8), the sealing plate (7) seals the opening of the detection cavity (8). The fluorescence receiving part (14) comprises a shell (22), and the bottom of the shell (22) is provided with a filter group (27).

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