Solution exchange device for extracting active recombinant protein

Abstract

The utility model relates to the technical field of exchange devices, and discloses an active recombinant protein extraction solution exchange device which comprises a mounting frame, an exchange frame is slidably connected to the left side in the mounting frame, a sliding groove is formed in the left side of the lower portion of the mounting frame, and a discharging pipe is fixedly connected to the lower portion of the exchange frame. A control valve is fixedly connected to the outer side of the discharging pipe, a first feeding pipe is fixedly connected to the left side of the upper portion of the mounting frame, a first valve is fixedly connected to the outer side of the first feeding pipe, a second feeding pipe is fixedly connected to the middle of the upper portion of the mounting frame, a second valve is fixedly connected to the outer side of the second feeding pipe, and an exchange assembly is arranged in the exchange frame. The device is used for solution exchange. According to the solution exchange device, through cooperation of the control valve, the handle, the exchange frame, the installation frame, the bolts, the connecting blocks, the installation blocks, the dialysis bags and other structures, the solution exchange device is convenient to disassemble the dialysis bags, and the replacement and cleaning difficulty of the dialysis bags of the solution exchange device is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of exchange device, especially to a solution exchange device for active recombinant protein extraction. BACKGROUND

[0002] Active recombinant protein extraction technology is a technology widely used in the field of biopharmaceuticals, one of the key steps of which is the solution exchange process. The purpose of solution exchange is to replace or dilute certain components (such as salt, buffer, etc.) in the extraction solution in order to effectively extract the target protein and ensure its activity. In order to complete this process, a specially designed solution exchange device is needed.

[0003] The existing solution exchange device usually includes a dialysis bag for containing the target protein extraction solution. By controlling the inflow and outflow of fluid, the liquid components in the dialysis bag can be exchanged. However, the conventional solution exchange device often has some inconvenience in terms of dialysis bag replacement and cleaning operation, which may affect the service life and performance of the equipment. SUMMARY

[0004] In order to make up for the above shortcomings, the utility model provides a solution exchange device for active recombinant protein extraction, aiming at improving the problem of large difficulty in replacing and cleaning the dialysis bag of the solution exchange device in the prior art.

[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme: a solution exchange device for active recombinant protein extraction, comprising a mounting frame, a exchange frame is slidably connected to the left side inside the mounting frame, a sliding groove is formed in the left lower part of the mounting frame, a discharge pipe is fixedly connected to the lower part of the exchange frame, a control valve is fixedly connected to the outside of the discharge pipe, a feed pipe one is fixedly connected to the left upper part of the mounting frame, a valve one is fixedly connected to the outside of the feed pipe one, a feed pipe two is fixedly connected to the upper middle part of the mounting frame, a valve two is fixedly connected to the outside of the feed pipe two, an exchange assembly is arranged inside the exchange frame for solution exchange.

[0006] As a further description of the above technical scheme:

[0007] The exchange assembly comprises a mounting block, which is fixedly connected to the upper part of the front and rear inner walls of the exchange frame, a connecting block is slidably connected inside the mounting block, a dialysis bag is fixedly connected to the opposite side of the connecting block, and a bolt is threadedly connected to the middle part of the connecting block.

[0008] As a further description of the above technical scheme:

[0009] The mounting frame is made of stainless steel, the exchange frame is made of polypropylene, and the feed pipe one and the feed pipe two are both made of polyethylene.

[0010] As a further description of the above technical solutions:

[0011] The left and right sides of the exchange frame are fixedly connected with handles.

[0012] As a further description of the above technical solutions:

[0013] The lower left and right sides of the mounting frame are fixedly connected with support frames, and the support frames are made of stainless steel.

[0014] As a further description of the above technical solutions:

[0015] The connecting block is connected with the mounting block through bolts.

[0016] As a further description of the above technical solutions:

[0017] The dialysis bag is made of polyester film material, and the bolts are made of stainless steel.

[0018] The utility model has the advantages of the following beneficial effects:

[0019] 1、 the utility model discloses a control valve is opened to the buffer solution is discharged, and the handle is pulled to make the handle drive exchange frame to separate from the mounting frame, and the bolt is rotated to make the discharge pipe to separate from the connecting block, and the connecting block is pulled to make the connecting block to separate from the mounting block, and the dialysis bag is pulled to make the dialysis bag to separate from the exchange frame, so as to realize that the solution exchange device is convenient for the dialysis bag to be disassembled, and the difficulty of replacement and cleaning of the dialysis bag of the solution exchange device is reduced.

[0020] 2、 the utility model discloses a mounting frame is provided with good mechanical strength, corrosion resistance and long-term stability by adopting stainless steel material, the chemical stability of exchange frame is guaranteed by adopting polypropylene material, and good corrosion resistance is provided for feed pipe one and feed pipe two by adopting polyethylene material, so as to improve the service life of the solution exchange device. BRIEF DESCRIPTION OF DRAWINGS

[0021] Fig. 1 A perspective view of the solution exchange device for extracting active recombinant protein is provided for the utility model;

[0022] Fig. 2 A bottom view of the solution exchange device for extracting active recombinant protein is provided for the utility model;

[0023] Fig. 3 An exchange frame schematic view of the solution exchange device for extracting active recombinant protein is provided for the utility model.

[0024] LEGEND:

[0025] 1, mounting frame; 2, support frame; 3, exchange frame; 4, sliding groove; 5, discharge pipe; 6, control valve; 7, handle; 8, feed pipe one; 9, valve one; 10, feed pipe two; 11, valve two; 12, mounting block; 13, connecting block; 14, dialysis bag; 15, bolt. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0027] REFERENCE Figs. 1-3 An embodiment provided by the utility model: a solution exchange device for active recombinant protein extraction, comprising a mounting frame 1, the left and right sides of the lower part of the mounting frame 1 are fixedly connected with support frames 2, the support frames 2 are made of stainless steel, the left side of the inside of the mounting frame 1 is slidably connected with an exchange frame 3, the left and right sides of the exchange frame 3 are fixedly connected with handles 7, the left side of the lower part of the mounting frame 1 is provided with a sliding groove 4, the lower part of the exchange frame 3 is fixedly connected with a discharge pipe 5, the outer side of the discharge pipe 5 is fixedly connected with a control valve 6, the upper left side of the mounting frame 1 is fixedly connected with a feed pipe one 8, the outer side of the feed pipe one 8 is fixedly connected with a valve one 9, the upper middle part of the mounting frame 1 is fixedly connected with a feed pipe two 10, the outer side of the feed pipe two 10 is fixedly connected with a valve two 11, the inside of the exchange frame 3 is provided with an exchange assembly for solution exchange, the exchange assembly comprises mounting blocks 12, the mounting blocks 12 are fixedly connected on the inner walls of the exchange frame 3 on the upper parts of the front and back sides, the inside of the mounting block 12 is slidably connected with a connecting block 13, the opposite side of the connecting block 13 is fixedly connected with a dialysis bag 14, the middle part of the connecting block 13 is threadedly connected with a bolt 15, and the connecting block 13 is connected with the mounting block 12 through the bolt 15.

[0028] When active recombinant protein extraction is needed, first, the valve one 9 is controlled to be opened to add buffer solution into the exchange frame 3 through the feed pipe one 8, then the valve two 11 is controlled to be opened to add protein solution into the exchange frame 3 through the feed pipe two 10, so that the salt or other small molecular substances in the protein solution are transferred into the buffer solution through the diffusion effect of the membrane, thereby realizing solution exchange, then the control valve 6 is controlled to be opened to discharge the buffer solution from the exchange frame 3, the handle 7 is pulled to drive the exchange frame 3 to separate from the mounting frame 1, the bolt 15 is rotated to make the discharge pipe 5 separate from the connecting block 13, then the connecting block 13 is pulled to separate from the mounting block 12, finally the dialysis bag 14 is pulled to separate from the exchange frame 3, so that the dialysis bag 14 is disassembled, the difficulty of replacement and cleaning of the dialysis bag 14 is reduced, and the maintainability and use convenience of the solution exchange device are improved.

[0029] Referring to Figs. 1-3 , the mounting frame 1 is made of stainless steel, the exchange frame 3 is made of polypropylene, the feed pipe one 8 and the feed pipe two 10 are both made of polyethylene, the dialysis bag 14 is made of polyester film, and the bolt 15 is made of stainless steel.

[0030] The stainless steel material provides good mechanical strength, corrosion resistance and long-term stability for the mounting frame 1, the polypropylene material ensures the chemical stability of the exchange frame 3, the polyethylene material provides good corrosion resistance for the feed pipe one 8 and the feed pipe two 10, thereby improving the service life of the solution exchange device, the polyester film material provides good air permeability and chemical stability for the dialysis bag 14, and the stainless steel material ensures the strength and corrosion resistance of the bolt 15.

[0031] Working principle: when active recombinant protein extraction is needed, the valve one 9 is controlled to be opened, the buffer solution is added to the exchange frame 3 through the feed pipe one 8, then the valve two 11 is controlled to be opened, the protein solution is added to the exchange frame 3 through the feed pipe two 10, the salt or other small molecules in the protein solution are diffused to the outside buffer solution through the membrane, the solution exchange is realized, the control valve 6 is controlled to be opened to discharge the buffer solution, finally the handle 7 is pulled to drive the exchange frame 3 to separate from the mounting frame 1, the discharge pipe 5 is separated from the connecting block 13 by rotating the bolt 15, the connecting block 13 is separated from the mounting block 12 by pulling the connecting block 13, and the dialysis bag 14 is separated from the exchange frame 3 by pulling the dialysis bag 14, so that the solution exchange device is convenient for disassembling the dialysis bag 14, and the difficulty of replacing and cleaning the dialysis bag 14 of the solution exchange device is reduced.

[0032] Finally, it should be pointed out that: the above only describes the preferred embodiments of the present application, and is not used to limit the present application, although the present application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, the technical solutions recorded in the foregoing embodiments can be modified, or some technical features can be replaced, any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A solution exchange device for the extraction of active recombinant proteins comprising a mounting frame (1), characterised in that: The left side of the mounting frame (1) is slidably connected with an exchange frame (3), the left lower part of the mounting frame (1) is provided with a sliding groove (4), the lower part of the exchange frame (3) is fixedly connected with a discharge pipe (5), the outer side of the discharge pipe (5) is fixedly connected with a control valve (6), the left upper part of the mounting frame (1) is fixedly connected with a feeding pipe one (8), the outer side of the feeding pipe one (8) is fixedly connected with a valve one (9), the upper middle part of the mounting frame (1) is fixedly connected with a feeding pipe two (10), the outer side of the feeding pipe two (10) is fixedly connected with a valve two (11), the exchange frame (3) is internally provided with an exchange assembly for solution exchange.

2. A solution exchange device for extracting active recombinant proteins according to claim 1, characterized in that: The exchange assembly comprises mounting blocks (12), which are fixedly connected to the inner walls of the exchange frame (3) on the upper parts of the front and rear sides, the mounting blocks (12) are slidably connected with connecting blocks (13) inside, the connecting blocks (13) are fixedly connected with dialysis bags (14) on the opposite sides, and the connecting blocks (13) are screwedly connected with bolts (15) in the middle parts.

3. The solution exchange device for extraction of active recombinant proteins according to claim 1, characterized in that: The mounting frame (1) is made of stainless steel, the exchange frame (3) is made of polypropylene, and the feeding pipe one (8) and the feeding pipe two (10) are both made of polyethylene.

4. The solution exchange device for extraction of active recombinant proteins according to claim 1, characterized in that: The left and right sides of the exchange frame (3) are both fixedly connected with handles (7).

5. The solution exchange device for extraction of active recombinant proteins according to claim 1, characterized in that: The left and right sides of the lower part of the mounting frame (1) are both fixedly connected with support frames (2), and the support frames (2) are made of stainless steel.

6. The solution exchange device for extraction of active recombinant proteins according to claim 2, characterized in that: The connecting blocks (13) are connected with the mounting blocks (12) through the bolts (15).

7. The solution exchange device for extraction of active recombinant proteins according to claim 2, characterized in that: The dialysis bags (14) are made of polyester film, and the bolts (15) are made of stainless steel.

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