Peak shape forward delay inhibition structure for detecting ritonavir intermediate liquid chromatography

Abstract

The utility model discloses a peak shape forward delay inhibition structure for detecting a ritonavir intermediate liquid chromatogram, which belongs to the technical field of chromatographic analysis and comprises a flow mixing tube and a filter unit, the flow mixing tube comprises a curved tube arranged in the flow mixing tube, a liquid mixing channel is arranged on the curved tube close to the center of the flow mixing tube, and the filter unit is arranged in the flow mixing tube. Each filtering unit comprises inlet channels formed in the two ends of the flow mixing pipe, positioning blocks are placed in the inlet channels, positioning grooves are formed in the positions, close to the outer sides of the inlet channels, of the positioning blocks, positioning plates are inserted into the positioning grooves, L-shaped plates are fixedly installed on the inner walls of the positioning plates, and the L-shaped plates are fixedly connected with the inner walls of the L-shaped plates. The device has the advantages that liquid can be shunted and enters inwards through the four curved pipes, a certain accelerated speed can be provided after the liquid enters the device, so that the flowability is faster and stronger, and finally, the peak shape is improved, the abnormal peak shape is eliminated, and the analysis result is more accurate.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of chromatographic analysis, concretely to a peak shape front extension inhibiting structure for detecting ritonavir intermediate liquid chromatography. BACKGROUND

[0002] Ritonavir is a protease inhibitor, mainly used for the treatment of HIV infection. Its mechanism of action is mainly through the formation of covalent complex with protease active site, blocking the proteolysis process, thereby interfering with viral replication. Using ritonavir intermediate can relieve the symptoms caused by the immune system damage of AIDS patients, such as persistent fever, diarrhea, weight loss, etc.

[0003] High performance liquid chromatography is an important branch of chromatography, which has the characteristics of high pressure, high efficiency, high sensitivity, wide application range, fast analysis speed and fast carrier liquid flow rate, and has obvious effect in component analysis, drug separation and purification, and is widely used in food, chemical industry, pharmacy and other fields.

[0004] The implementation basis of high performance liquid chromatography is liquid chromatograph, and the liquid is used as mobile phase during operation. The single solvent or mixed solvent with different proportions, buffer solution and other mobile phases stored in the liquid reservoir are pumped into the chromatographic column filled with stationary phase by using high pressure liquid delivery system. Because each component in the sample solution has different partition coefficients in the two phases, there will be a big difference in the moving speed when each component moves in the two phases, and then the single component is separated in the column and enters the detector for detection, realizing the analysis of the sample.

[0005] In order to obtain symmetrical peak shape and optimal separation efficiency when using liquid chromatography for analysis and preparation, the sample is usually dissolved with mobile phase, which can avoid the abnormal phenomena such as peak extension, broadening and bifurcation caused by solvent effect (chromatographic peak deformation phenomenon caused by diluent solvent strength greater than mobile phase). However, it is found that the solubility of many samples in the mobile phase is poor. Based on the limitation of objective conditions, it is often necessary to select a solvent different from the mobile phase to dissolve the sample. The sample solvent actually plays the role of "mobile phase" during the sampling process. Although the action time is very short, because the concentration of the sample is very high at this time, the influence of the sample solvent on the chromatographic peak shape is very large, which may cause the phenomenon of peak shape extension. The quality of the peak shape further affects the chromatographic separation result and the accuracy of the analysis result.

[0006] It is still a problem to be solved to further optimize the liquid mixing structure in the inhibiting structure and effectively improve the effect of online dilution of sample solution by mobile phase.

[0007] Based on this, it is necessary to provide a kind of dilution work of sample solution can be completed in short time, help to eliminate the problem of peak abnormality and inaccurate analysis result caused by solvent effect. Utility model content

[0008] The utility model discloses a peak shape front extension inhibiting structure for detecting ritonavir intermediate liquid chromatography, to at least partly solve the above-mentioned technical problem, to more quickly complete solution dilution work, reduce the problem of peak abnormality and inaccurate analysis result.

[0009] To achieve the above object, the utility model provides the following technical scheme: including mixed flow pipe and filter unit, the mixed flow pipe includes the curved pipe being arranged in the mixed flow pipe interior, the curved pipe is close to the mixed liquid passageway being provided with in the mixed flow pipe center position;

[0010] The filter unit includes the entering passageway being opened in the both ends of the mixed flow pipe, the entering passageway is placed with the positioning block in the inside, the positioning block is opened with the positioning slot in the outside department of the entering passageway, the positioning slot is inserted with the positioning board in the inside, the inner wall of the positioning board is fixedly installed with L type board, the L type board is fixedly installed with the sieve plate in the end away from the positioning board.

[0011] Preferably, the mixed flow pipe further includes a hexagon nut for connecting structural devices.

[0012] Preferably, the curved pipe is opened in the both ends of the mixed flow pipe respectively, and the curved pipe is curvedly arranged in a circular pipeline.

[0013] Preferably, the curved pipe is arranged in four at the both ends of the mixed flow pipe, and the curved pipe away from the hexagon nut is in the mixed liquid passageway.

[0014] Preferably, the side area of the curved pipe is less than the side area of the sieve plate.

[0015] Preferably, the filter unit is symmetrically arranged at the both ends of the mixed flow pipe with the center of the mixed flow pipe.

[0016] Compared with the prior art, the utility model has the advantages that:

[0017] 1, the positioning block is placed in the entering passageway, the positioning board is inserted into the positioning slot, so that the sieve plate is in the positioning block, can be filtered and intercepted, then one end of the mixed flow pipe is connected with the device through the hexagon nut, and the other end of the mixed flow pipe is also connected with the device through the hexagon nut, so that the filter unit is convenient for intercepting and blocking large particles, and convenient for disassembly and cleaning.

[0018] 2. When this utility model is used, the liquid can be diverted into the inward flow through four curved tubes. The inlet of the curved tubes is convex, which can provide a certain acceleration after entering, making the flow faster and stronger. It can be more quickly merged and diluted at the mixing channel, ultimately improving the peak shape, eliminating peak shape abnormalities, and making the analysis results more accurate. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Fig. 1 This is a schematic diagram of the overall structure of this embodiment.

[0021] Fig. 2 This is a schematic diagram of the internal structure of the overall structure in this embodiment;

[0022] Fig. 3 This is a cross-sectional view of one end of the mixing tube structure in this embodiment.

[0023] The attached diagram lists the components represented by each number as follows:

[0024] 100. Mixing tube; 110. Inlet channel; 120. Curved tube; 130. Mixing channel; 140. Hexagonal nut;

[0025] 200, Filter unit; 210, Positioning block; 211, Positioning groove; 220, Positioning plate; 230, L-shaped plate; 240, Screen plate. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figs. 1-3 This utility model provides a technical solution: a peak-leading inhibition structure for detecting ritonavir intermediates in liquid chromatography, including a mixing tube 100 and a filter unit 200. The mixing tube 100 also includes a hexagonal nut 140 for connecting structural components. The hexagonal nut 140 can be used to connect the mixing tube 100 to other components, which is convenient for disassembly and assembly.

[0028] The mixed flow pipe 100 comprises the curved pipes 120 arranged inside the mixed flow pipe 100, the curved pipes 120 are respectively arranged at two ends of the mixed flow pipe 100, the curved pipes 120 are arranged in a curved shape of a circular pipe, and the mixed liquid channels 130 are arranged at positions close to the center of the mixed flow pipe 100.

[0029] The plurality of curved pipes 120 can inject the flow phase from the multi-channel, reduce the inflow area, increase the flow rate, the curved arrangement of the curved pipes 120 can provide faster acceleration, so that the flow phase enters the mixed liquid channel 130 faster, so that the flow phase and the solution collide sharply to mix, and the liquid is diluted faster, and the four curved pipes 120 are arranged at two ends of the mixed flow pipe 100, and the four curved pipes 120 are arranged in the mixed liquid channel 130 away from one end of the hexagonal nut 140, and the curved pipes 120 can be injected from two ends of the mixed flow pipe 100.

[0030] Referring to Figs. 1-3 In a preferred embodiment, the filter unit 200 comprises the inlet channels 110 arranged at two ends of the mixed flow pipe 100, the positioning blocks 210 are arranged inside the inlet channels 110, the positioning grooves 211 are arranged at outer sides of the inlet channels 110, the positioning plates 220 are inserted into the positioning grooves 211, the L-shaped plates 230 are fixedly installed on inner walls of the positioning plates 220, the sieve plates 240 are fixedly installed on one end of the L-shaped plates 230 away from the positioning plates 220, the side area of the curved pipes 120 is smaller than the side area of the sieve plates 240, and the filter unit 200 is symmetrically arranged at two ends of the mixed flow pipe 100 with the center of the mixed flow pipe 100 as the center.

[0031] The positioning blocks 210, the positioning grooves 211 and the positioning plates 220 in the filter unit 200 are all arranged in a circular opening, so that the filter unit 200 can be conveniently arranged in the inlet channels 110 in the mixed flow pipe 100, the sieve plates 240 can conveniently perform interception and filtration work, and the sieve plates 240 can be conveniently disassembled and replaced, the side area of the sieve plates 240 is greater than the side area of the curved pipes 120, so that the sieve plates 240 can intercept large particles during work, and prevent the large particles from protruding into the curved pipes 120.

[0032] In the description of the utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inner", "front", "central", "two ends" and the like indicate the orientation or positional relationship shown in the drawings, and are only used for the convenience of describing the utility model and simplifying the description, and cannot be understood as a limitation on the utility model.

[0033] In the utility model, unless another definite provision and limitation, the term "installation", "arrangement", "connection", "fixation", "screw connection" and so on term should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or be integrated;Can be mechanical connection, also can be electrical connection;Can be direct connection, also can indirectly connect through intermediate medium, can be two element inside's intercommunication or two element's mutual action relation, unless another definite limitation, for the ordinary skill of the art, can understand the above-mentioned term in the utility model specific meaning according to specific circumstances.

[0034] Although the embodiments of the utility model have been shown and described, it will be appreciated by those of ordinary skill in the art that various changes, modifications, alternatives, and variations can be made thereto without departing from the principles and spirit of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A peak shape front extension inhibiting structure for detecting ritonavir intermediate liquid chromatography, comprising a mixing pipe (100) and a filtering unit (200), characterized in that: the mixing pipe (100) comprises a meandering pipe (120) arranged inside the mixing pipe (100), and a mixed liquid passage (130) is arranged near the center of the mixing pipe (100); the filtering unit (200) comprises an entering passage (110) arranged at both ends of the mixing pipe (100), a positioning block (210) is arranged inside the entering passage (110), a positioning groove (211) is arranged on the outer side of the entering passage (110), a positioning plate (220) is inserted into the positioning groove (211), an L-shaped plate (230) is fixedly installed on the inner wall of the positioning plate (220), and a sieve plate (240) is fixedly installed on the end of the L-shaped plate (230) away from the positioning plate (220). The mixing pipe (100) further comprises a hexagonal nut (140) for connecting structural devices.

2. A peak shape front extension inhibiting structure for liquid chromatography for detecting ritonavir intermediate according to claim 1, characterized in that: The meandering pipes (120) are respectively arranged at both ends of the mixing pipe (100), and the meandering pipes (120) are arranged in a circular pipe bending mode.

3. The peak shape front extension inhibiting structure for liquid chromatography for detecting ritonavir intermediate according to claim 1, characterized by: There are four meandering pipes (120) at each end of the mixing pipe (100), and the ends of the four meandering pipes (120) away from the hexagonal nut (140) are in the mixed liquid passage (130).

4. The peak shape front extension inhibiting structure for liquid chromatography for detecting ritonavir intermediate according to claim 2, characterized by: The side area of the meandering pipe (120) is smaller than the side area of the sieve plate (240).

5. The peak shape front extension inhibiting structure for liquid chromatography for detecting ritonavir intermediate according to claim 1, characterized by: The filtering unit (200) is symmetrically arranged at both ends of the mixing pipe (100) with the center of the mixing pipe (100) as the center.

6. The peak shape front extension inhibiting structure for liquid chromatography for detecting ritonavir intermediate according to claim 1, characterized by: ​

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