Polypeptide solid-phase reaction kettle
By adding a vessel body and sieve plate to the reactor, the problem of peptide resin spillage during disassembly at the bottom of the vessel was solved, enabling safe removal of peptide resin and convenient maintenance of the equipment.
Patent Information
- Application Number
- CN202423150964.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2026-01-20
- Estimated Expiration
- 2034-12-19
AI Technical Summary
When disassembling the bottom of a traditional reactor, peptide resin is prone to spillage along the edge of the bottom, resulting in losses.
Design a peptide solid-phase reaction vessel, including a vessel body, a vessel frame, and a vessel bottom. A sieve plate is added between the vessel frame and the vessel bottom. Peptide resin is contained in the bottom of the vessel frame. It is not easy to spill when the vessel frame is separated from the vessel frame. The vessel frame and the vessel bottom are detachably connected to facilitate the removal of peptide resin.
It effectively prevents peptide resin from spilling during disassembly, facilitates the removal of peptide resin, and makes sieve plate maintenance easier, thus improving the reliability and efficiency of operation.
Smart Images
Figure CN223811038U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to polypeptide solid phase reaction technical field, concretely relates to a polypeptide solid phase reaction kettle. BACKGROUND
[0002] Polypeptide synthesis is a solid phase synthesis sequence generally from N end (amino end) to C end (carboxyl end) synthesis, and the solid phase polypeptide synthesis method is a commonly used technology in polypeptide and protein synthesis. The reaction kettle is used in the process of solid phase polypeptide synthesis, resin is added as reaction carrier and proper solvent material to the reaction kettle, and stirring reaction is carried out at the set temperature, so that amino acid raw materials are bonded on the resin to obtain peptide resin, and the kettle bottom of the traditional reaction kettle is designed to be separated from the kettle body, but the peptide resin in the kettle bottom is easy to leak out along the edge of the kettle bottom when the kettle bottom is disassembled, which causes unnecessary loss. SUMMARY
[0003] Therefore, the utility model provides a polypeptide solid phase reaction kettle to solve the problem that the peptide resin in the kettle bottom is easy to leak out along the edge of the kettle bottom when the kettle bottom is disassembled.
[0004] The utility model provides a polypeptide solid phase reaction kettle, which comprises:
[0005] The kettle main body comprises a kettle body, a kettle body and a kettle bottom which are detachably connected in sequence from top to bottom along a first direction, the kettle bottom is provided with a mounting groove relative to the end face close to the kettle body, the mounting groove is provided with a sieve plate, the end face outer edge of the sieve plate relative to the kettle body abuts against the kettle body, and part of the interior of the kettle body is used for accommodating peptide resin;
[0006] The stirring assembly is arranged in the kettle main body.
[0007] The polypeptide solid phase reaction kettle has at least the following beneficial effects:
[0008] The kettle body and the kettle bottom are provided between the kettle body and the kettle bottom, and the peptide resin obtained by reaction is accommodated in the inner bottom of the kettle body, in the process of taking out the peptide resin from the kettle main body, the kettle body and the kettle body are first separated, at this time, the peptide resin is accommodated in the inner bottom of the kettle body, and is not easy to leak out along the connection between the kettle body and the kettle body; the upper end of the whole composed of the kettle body and the kettle bottom is open, which is convenient for taking out the peptide resin from the kettle bottom without leakage; meanwhile, the bottom end of the sieve plate abuts against the inner bottom wall of the mounting groove, and the top end abuts against the inner bottom wall of the kettle body, so that the sieve plate is stably assembled between the kettle body and the kettle bottom, and is not easy to move during stirring synthesis or taking out the peptide resin; the kettle body and the kettle bottom are detachably connected into a whole, which is convenient for disassembling the kettle body and the kettle bottom to take out the sieve plate for maintenance.
[0009] In an alternative embodiment, the outer circumferential surface of the kettle body is sleeved with a first temperature control assembly; the first temperature control assembly comprises a first heat exchange jacket sleeved on the outer circumferential surface of the kettle body, and the inside of the first heat exchange jacket is provided with a first temperature control circulating liquid space for the temperature control circulating liquid to flow through; the lower end of the outer lateral wall of the first heat exchange jacket is provided with a first liquid inlet communicating with the first temperature control circulating liquid space; and the upper end of the outer lateral wall of the first heat exchange jacket is provided with a first liquid outlet communicating with the first temperature control circulating liquid space.
[0010] In an alternative embodiment, the outer circumferential surface of the kettle body is sleeved with a second temperature control assembly; the second temperature control assembly comprises a second heat exchange jacket sleeved on the outer circumferential surface of the kettle body, and the inside of the second heat exchange jacket is provided with a second temperature control circulating liquid space for the temperature control circulating liquid to flow through; the upper end of the outer lateral wall of the second heat exchange jacket is provided with a second liquid inlet communicating with the second temperature control circulating liquid space; and the lower end of the outer lateral wall of the second heat exchange jacket is provided with a second liquid outlet communicating with the second temperature control circulating liquid space.
[0011] In an alternative embodiment, the bottom of the kettle bottom is provided with a drain pipe communicating with the bottom, and the drain pipe is provided with a valve.
[0012] In an alternative embodiment, the bottom of the kettle main body is provided with a universal wheel, and the universal wheel is locked by a foot pedal locking member.
[0013] In an alternative embodiment, the stirring assembly comprises a stirring paddle arranged in the kettle body, and the stirring paddle is driven by a motor to rotate around the axis of the kettle main body.
[0014] In an alternative embodiment, the outer lateral wall of one end of the kettle body close to the kettle body is provided with a first connecting table, the outer lateral wall of one end of the kettle body close to the kettle body is provided with a second connecting table, the first connecting table and the second connecting table are connected by bolts, and the first sealing ring is arranged between the kettle body and the kettle body.
[0015] In an alternative embodiment, the end surface of the kettle body abutting against the kettle body is provided with a first embedding groove, the first embedding groove is arranged around the circumference, the upper end of the first sealing ring is embedded in the first embedding groove, the end surface of the kettle body abutting against the kettle body is provided with a second embedding groove, the second embedding groove is arranged around the circumference, and the lower end of the first sealing ring is embedded in the second embedding groove.
[0016] In an alternative embodiment, the outer side wall of the kettle body relative to the end close to the kettle bottom is provided with a third connecting table, the outer side wall of the kettle bottom relative to the end close to the kettle body is provided with a fourth connecting table, and the third connecting table and the fourth connecting table are connected by bolts.
[0017] In an alternative embodiment, the end face of the kettle body and the kettle bottom is provided with a third embedding groove, the third embedding groove is arranged around the circumference, and the upper end of the second sealing ring is embedded in the third embedding groove; the end face of the kettle bottom and the kettle body is provided with a fourth embedding groove, the fourth embedding groove is arranged around the circumference, and the lower end of the second sealing ring is embedded in the fourth embedding groove. BRIEF DESCRIPTION OF DRAWINGS
[0018] In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the drawings needed in the specific embodiments or prior art description will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.
[0019] Figure 1 The structure of a polypeptide solid-phase reaction kettle according to an embodiment of the present application is shown in the figure.
[0020] Explanation of reference signs:
[0021] 100-kettle body, 110-kettle body, 111-first connecting table, 112-feeding port, 120-kettle body, 121-second connecting table, 122-third connecting table, 130-kettle bottom, 131-fourth connecting table, 140-sieve plate, 150-liquid discharge pipe, 160-universal wheel;
[0022] 210-first heat exchange jacket, 220-first liquid inlet, 230-first liquid outlet;
[0023] 310-second heat exchange jacket, 320-second liquid inlet, 330-second liquid outlet;
[0024] 410-stirring paddle. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme of the embodiments of the utility model will be clearly and completely described below in combination with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the protection scope of the utility model.
[0026] In the description of the embodiments, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the embodiments and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.
[0027] In the description of the embodiments, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For the person skilled in the art, the specific meaning of the above terms in the embodiments can be understood according to the specific circumstances.
[0028] The embodiments of the utility model will be described below in combination with Figure 1 .
[0029] According to the polypeptide solid-phase reaction kettle provided by the embodiments of the utility model, the kettle body 100 includes kettle body 110, kettle body 120 and kettle bottom 130 which are detachably connected in sequence from top to bottom along the first direction, the end face of the kettle bottom 130 relatively close to the kettle body 120 is provided with a mounting groove, the mounting groove is provided with sieve plate 140, the end face outer edge of the sieve plate 140 relatively close to the kettle body 120 abuts against the kettle body 120, part of the inside of the kettle body 120 is used for accommodating peptide resin, and the stirring assembly is arranged in the kettle body 100.
[0030] The polypeptide solid-phase reaction kettle of the embodiment is characterized in that the kettle body 110 and the kettle bottom 130 are additionally provided with the kettle body 120, and the obtained peptide resin is contained in the inner bottom of the kettle body 120. In the process of taking out the peptide resin from the kettle main body 100, the kettle body 110 and the kettle body 120 are first separated, at this time, the peptide resin is contained in the inner bottom of the kettle body 120, and is not easy to leak out along the connection between the kettle body 120 and the kettle body 110. Then, the upper end of the whole composed of the kettle body 120 and the kettle bottom 130 is open, which is beneficial to conveniently take out the peptide resin from the kettle bottom 130 without the phenomenon of spilling. At the same time, because the bottom end of the sieve plate 140 abuts against the inner bottom wall of the installation groove, and the top end abuts against the inner bottom wall of the kettle body 120, the sieve plate 140 is stably assembled between the kettle body 120 and the kettle bottom 130, and is not easy to move during stirring synthesis or taking out the peptide resin. In addition, the kettle body 120 and the kettle bottom 130 are detachably connected into a whole, which is beneficial to disassemble the kettle body 120 and the kettle bottom 130 to take out the sieve plate 140 for maintenance.
[0031] It should be noted that, compared with the traditional reaction kettle, the resin is poured from the top of the kettle before the reaction starts, and the resin is easy to stick to the kettle wall during the pouring process. In the embodiment, the resin is first poured into the whole composed of the kettle body 120 and the kettle bottom 130, and then the kettle body 120 and the kettle body 110 are connected into a whole. Because the upper end of the whole composed of the kettle body 120 and the kettle bottom 130 is open and the depth is relatively shallow, the resin is not easy to stick to the kettle wall during the pouring process.
[0032] It can be understood that the first direction mentioned in the text refers to the axis direction of the kettle main body 100. In order to facilitate the understanding of the description, the first direction shown in the embodiment is taken as the first direction, but it should not be understood as the explicit limitation of the first direction. Figure 1
[0033] In some embodiments, the kettle body 110 is provided with a first temperature control assembly outside the outer circumferential surface of the kettle body 110. The first temperature control assembly comprises a first heat exchange jacket 210 provided outside the outer circumferential surface of the kettle body 110. The first heat exchange jacket 210 is internally provided with a first temperature control circulating liquid space for the temperature control circulating liquid to flow through. The lower end of the outer lateral wall of the first heat exchange jacket 210 is provided with a first liquid inlet 220, which is connected to the first temperature control circulating liquid space. The upper end of the outer lateral wall of the first heat exchange jacket 210 is provided with a first liquid outlet 230, which is connected to the first temperature control circulating liquid space. During the reaction, the high-temperature or low-temperature temperature control circulating liquid is input into the first temperature control circulating liquid space through the first liquid inlet 220 and exchanges heat with the solution in the kettle body 110 through the inner wall of the kettle body 110, and then flows out through the first liquid outlet 230. The high-temperature or low-temperature temperature control circulating liquid continuously flows in the first temperature control circulating liquid space for heat exchange, so that the solution in the kettle body 100 is kept at the required temperature for the reaction.
[0034] Specifically, the kettle body 120 is provided with a second temperature control assembly outside the outer circumferential surface of the kettle body 120. The second temperature control assembly comprises a second heat exchange jacket 310 provided outside the outer circumferential surface of the kettle body 120. The second heat exchange jacket 310 is internally provided with a second temperature control circulating liquid space for the temperature control circulating liquid to flow through. The upper end of the outer lateral wall of the second heat exchange jacket 310 is provided with a second liquid inlet 320, which is connected to the second temperature control circulating liquid space. The lower end of the outer lateral wall of the second heat exchange jacket 310 is provided with a second liquid outlet 330, which is connected to the second temperature control circulating liquid space. During the reaction, the high-temperature or low-temperature temperature control circulating liquid is input into the second temperature control circulating liquid space through the second liquid inlet 320 and exchanges heat with the solution in the kettle body 120 through the inner wall of the kettle body 120, and then flows out through the second liquid outlet 330. The high-temperature or low-temperature temperature control circulating liquid continuously flows in the first temperature control circulating liquid space and the second temperature control circulating liquid space for heat exchange, which is conducive to keeping the solution in the kettle body 100 at the required temperature for the reaction. At the same time, by arranging the first liquid inlet 220 at the lower end of the outer lateral wall of the first heat exchange jacket 210 and the second liquid inlet 320 at the upper end of the outer lateral wall of the second heat exchange jacket 310, and arranging the first liquid inlet 220 and the second liquid inlet 320 on the same side of the kettle body 100 perpendicular to the first direction, the first liquid inlet 220 and the second liquid inlet 320 can be detachably connected to the liquid outlet of the device providing the high-temperature or low-temperature temperature control circulating liquid. It should be noted that the device providing the high-temperature or low-temperature temperature control circulating liquid uses existing devices, such as devices containing circulating pipelines, circulating pumps and plate heat exchangers. No improvement is made here, so it will not be described in detail.
[0035] Specifically, the bottom of the kettle bottom 130 is provided with a liquid discharge pipe 150, and the liquid discharge pipe 150 is provided with a valve. The liquid discharge pipe 150 at the bottom facilitates the full discharge of the liquid in the kettle body 100, so as to facilitate the separation of the kettle body 110 and the kettle body 120, and the removal of the peptide resin from the whole composed of the kettle body 120 and the kettle bottom 130 without spilling.
[0036] Specifically, the bottom of the kettle body 100 is provided with universal wheels 160, and the universal wheels 160 are locked by a foot pedal locking member. By providing the universal wheels 160, after the reaction is completed and the connection between the kettle body 110 and the kettle body 120 is separated, the whole composed of the kettle body 120 and the kettle bottom 130 is facilitated to move in a direction perpendicular to the first direction to separate from the kettle body 110.
[0037] It should be noted that the foot pedal locking member is of a common type on the market, and the structure of the foot pedal locking member is not improved here, and will not be described here.
[0038] It can be understood that the kettle body 110 is installed on the ground by a rack, so that the whole composed of the kettle body 120 and the kettle bottom 130 is moved in a direction perpendicular to the first direction relative to the kettle body 110 to separate.
[0039] Specifically, the stirring assembly includes a stirring paddle 410 arranged in the kettle body 110, and the stirring paddle 410 is driven by a motor to rotate around the axis of the kettle body 100. The bottom of the stirring paddle 410 is hidden in the kettle body 110, which can normally stir the liquid in the kettle body 100 during the reaction, and the stirring paddle 410 does not interfere with the movement of the kettle body 120 when the whole composed of the kettle body 120 and the kettle bottom 130 is separated from the kettle body 110.
[0040] Specifically, the top end of the kettle body 100 is provided with a feeding port 112, which facilitates the feeding of other reaction materials into the kettle body 100 after the whole composed of the kettle body 120 and the kettle bottom 130 is connected to the kettle body 110.
[0041] In order to meet the need of adding more solution in a single reaction, specifically, the size of the kettle body 110 along the first direction is 2 to 3 times the size of the kettle body 120 along the first direction.
[0042] In some embodiments, the kettle body 110 is provided with a first connecting table 111 on the outer side wall of one end of the kettle body 110, the kettle body 120 is provided with a second connecting table 121 on the outer side wall of one end of the kettle body 120, and the first connecting table 111 and the second connecting table 121 are connected by bolts; a first sealing ring is arranged between the kettle body 110 and the kettle body 120. When the kettle body 110 and the kettle body 120 are connected into one body by bolts, the first sealing ring is in close contact with the kettle body 110 and the kettle body 120 respectively, which is conducive to improving the sealing connection performance of the contact and fit of the kettle body 110 and the kettle body 120.
[0043] Specifically, the end face of the kettle body 110 and the kettle body 120 is provided with a first embedding groove, the first embedding groove is arranged around the circumference, and the upper end of the first sealing ring is embedded in the first embedding groove; the end face of the kettle body 120 and the kettle body 110 is provided with a second embedding groove, the second embedding groove is arranged around the circumference, and the lower end of the first sealing ring is embedded in the second embedding groove. In the process of connecting the kettle body 110 and the kettle body 120 into one body by bolts, the first embedding groove and the second embedding groove cooperatively limit the position of the first sealing ring, reduce the risk of movement of the first sealing ring in the direction perpendicular to the first direction, and are conducive to improving the sealing connection performance of the contact and fit of the kettle body 110 and the kettle body 120.
[0044] Specifically, the kettle body 120 is provided with a third connecting table 122 on the outer side wall of one end of the kettle body 120, the kettle body 130 is provided with a fourth connecting table 131 on the outer side wall of one end of the kettle body 130, and the third connecting table 122 and the fourth connecting table 131 are connected by bolts; a second sealing ring is arranged between the kettle body 120 and the kettle body 130. When the kettle body 120 and the kettle body 130 are connected into one body by bolts, the second sealing ring is in close contact with the kettle body 120 and the kettle body 130 respectively, which is conducive to improving the sealing connection performance of the contact and fit of the kettle body 120 and the kettle body 130.
[0045] Specifically, the end face of the kettle body 120 and the kettle body 130 is provided with a third embedding groove, the third embedding groove is arranged around the circumference, and the upper end of the second sealing ring is embedded in the third embedding groove; the end face of the kettle body 130 and the kettle body 120 is provided with a fourth embedding groove, the fourth embedding groove is arranged around the circumference, and the lower end of the second sealing ring is embedded in the fourth embedding groove. In the process of connecting the kettle body 120 and the kettle body 130 into one body by bolts, the third embedding groove and the fourth embedding groove cooperatively limit the position of the second sealing ring, reduce the risk of movement of the second sealing ring in the direction perpendicular to the first direction, and are conducive to improving the sealing connection performance of the contact and fit of the kettle body 120 and the kettle body 130.
[0046] Although the embodiments of the present application are described in conjunction with the drawings, various modifications and changes can be made by those skilled in the art without departing from the spirit and scope of the present application, and such modifications and changes are intended to fall within the scope of the present application defined by the appended claims.
Claims
1. A polypeptide solid phase reactor characterized in that, The utility model relates to a kind of peptide synthesis kettle, including: Kettle main body (100), including detachable connection in order from top to bottom along first direction kettle body (110), kettle body (120) and kettle bottom (130), the end face of the kettle bottom (130) is relatively close to the kettle body (120) and is provided with mounting groove, sieve plate (140) is arranged in the mounting groove, the end face outer edge of the sieve plate (140) is relatively close to the kettle body (120) and is abutted with the kettle body (120), part of the inside of the kettle body (120) is used to accommodate peptide resin; Stirring assembly is arranged in the kettle main body (100).
2. The polypeptide solid-phase reactor of claim 1, wherein, The outer peripheral surface of the kettle body (110) is sleeved with a first temperature control assembly; the first temperature control assembly includes a first heat exchange jacket (210) sleeved on the outer peripheral surface of the kettle body (110), and the inside of the first heat exchange jacket (210) is provided with a first temperature control circulating liquid space for the temperature control circulating liquid to flow through; the lower end of the outer lateral wall of the first heat exchange jacket (210) is provided with a first liquid inlet (220) communicating with the first temperature control circulating liquid space; and the upper end of the outer lateral wall of the first heat exchange jacket (210) is provided with a first liquid outlet (230) communicating with the first temperature control circulating liquid space.
3. The polypeptide solid phase reactor of claim 2, wherein, The outer peripheral surface of the kettle body (120) is sleeved with a second temperature control assembly, and the second temperature control assembly includes a second heat exchange jacket (310) sleeved on the outer peripheral surface of the kettle body (120); the inside of the second heat exchange jacket (310) is provided with a second temperature control circulating liquid space for the temperature control circulating liquid to flow through; the upper end of the outer lateral wall of the second heat exchange jacket (310) is provided with a second liquid inlet (320) communicating with the second temperature control circulating liquid space; and the lower end of the outer lateral wall of the second heat exchange jacket (310) is provided with a second liquid outlet (330) communicating with the second temperature control circulating liquid space.
4. The polypeptide solid-phase reactor according to any one of claims 1 to 3, characterized in that, The bottom of the kettle bottom (130) is provided with a drain pipe (150) in communication, and the drain pipe (150) is provided with a valve.
5. The polypeptide solid-phase reactor according to any one of claims 1 to 3, wherein, The bottom of the kettle main body (100) is provided with a universal wheel (160), and the universal wheel (160) is locked by a foot pedal locking member.
6. The polypeptide solid phase reactor of claim 5, wherein, The stirring assembly includes a stirring paddle (410) arranged in the kettle body (110), and the stirring paddle (410) is driven by a motor to rotate around the axis of the kettle main body (100).
7. The polypeptide solid-phase reaction vessel of claim 1, wherein the polypeptide is a protein. The outer lateral wall of one end of the kettle body (110) close to the kettle body (120) is protrudingly provided with a first connecting table (111), the outer lateral wall of one end of the kettle body (120) close to the kettle body (110) is protrudingly provided with a second connecting table (121), the first connecting table (111) and the second connecting table (121) are connected by bolts; and a first sealing ring is arranged between the kettle body (110) and the kettle body (120).
8. The polypeptide solid phase reactor of claim 7, wherein, The end face of the kettle body (110) abutting against the kettle body (120) is provided with a first embedding groove, the first embedding groove is arranged around a circle, and the upper end of the first sealing ring is embedded in the first embedding groove; the end face of the kettle body (120) abutting against the kettle body (110) is provided with a second embedding groove, the second embedding groove is arranged around a circle, and the lower end of the first sealing ring is embedded in the second embedding groove.
9. A polypeptide solid-phase reaction vessel according to claim 1 or 7, characterized in that, The outer side wall of one end of the kettle body (120) relatively close to the kettle bottom (130) is provided with a third connecting table (122), the outer side wall of one end of the kettle bottom (130) relatively close to the kettle body (120) is provided with a fourth connecting table (131), and the third connecting table (122) and the fourth connecting table (131) are connected through bolts; the second sealing ring is arranged between the kettle body (120) and the kettle bottom (130).
10. The polypeptide solid phase reactor of claim 9, wherein, The end face of the kettle body (120) abutting against the kettle bottom (130) is provided with a third embedding groove, the third embedding groove is arranged around a circle, and the upper end of the second sealing ring is embedded in the third embedding groove; the end face of the kettle bottom (130) abutting against the kettle body (120) is provided with a fourth embedding groove, the fourth embedding groove is arranged around a circle, and the lower end of the second sealing ring is embedded in the fourth embedding groove.