Optimized blastocyst free DNA extraction assembly

Abstract

The utility model discloses an optimized blastocyst free DNA (deoxyribonucleic acid) extraction component, which relates to the technical field of extraction instruments and comprises a hollow tube, a side tube is arranged on one side of the hollow tube, a connecting tube orifice is arranged at the top end of the hollow tube, a biopsy needle is arranged in the hollow tube, a plurality of adjusters are annularly distributed at the top end of the connecting tube orifice, and the adjusters are connected with the side tube. The outer surfaces of the adjusters are in threaded connection with nuts, clamping blocks are arranged at the top ends of the adjusters, and connecting pieces are detachably installed at the top ends of the adjusters. According to the utility model, a series of structures are arranged, and the connecting piece and the connecting pipe orifice are connected together through the regulator, so that the connecting pipe orifice does not need to be manually supported when an operating arm of a micromanipulation system operates a biopsy needle, the stability of the connecting pipe orifice is ensured, and when gas is conveyed through the side pipe, the gas output of the side pipe is regulated through the clearance holes with different apertures; therefore, the air outlet pressure is adjusted so as to blow away mineral oil with different thicknesses, and liquid cannot be blown to generate splashing.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of extraction instrument, concretely is a kind of optimized blastula free DNA extraction component. BACKGROUND

[0002] Preimplantation genetic testing (PGT) refers to the in vitro culture of embryos of patients with genetic risks to Day3, Day5 or Day6 during in vitro fertilization-embryo transfer, and biopsy of blastomeres or trophoblasts, and genetic testing of the biopsy samples to select embryos without genetic diseases for uterine cavity implantation, thereby obtaining a normal fetus, which is an assisted reproductive technology that can effectively prevent the birth of children with genetic diseases.

[0003] When the existing blastula free DNA extraction component operates the biopsy needle through the operation arm of the micro-operation system, the hollow tube needs to be held by the operator, and the stability is low. During operation, hand shaking may occur, affecting the extraction effect. Moreover, the existing blastula free DNA extraction component cannot adjust the air pressure at the bottom of the hollow end, and cannot blow air for mineral oil of different thicknesses, which may cause incomplete blowing of mineral oil or splashing of liquid. SUMMARY

[0004] The utility model aims at providing a kind of optimized blastula free DNA extraction component to solve the problems raised in the above background.

[0005] To achieve the above object, the utility model provides the following technical scheme: an optimized blastula free DNA extraction component, comprising a hollow tube, one side of the hollow tube is provided with a side pipe, the top end of the hollow tube is provided with a connecting pipe opening, a biopsy needle is arranged inside the hollow tube, the top end of the connecting pipe opening is annularly arranged with a plurality of adjusters, the outer surface of the plurality of adjusters is threadedly connected with a nut, the top end of the plurality of adjusters is provided with a clamping block, the top end of the plurality of adjusters is detachably mounted with a connecting piece, the inside of the connecting piece is provided with a clamping groove matched with the clamping block, the inside of the side pipe is provided with a top disc, the inside of the pipe at the bottom end of the top disc is provided with a turntable, the top side of the top disc is provided with an air outlet hole, and the top of the turntable is annularly arranged with a plurality of gap holes.

[0006] Preferably, the plurality of gap holes are circular holes with different hole diameters, and the plurality of gap holes are respectively coincided with the air outlet hole by rotating the turntable. By using the gap holes with different hole diameters and the air outlet hole together, the air outlet amount of the side pipe can be adjusted, and the air outlet pressure can be adjusted to blow open mineral oil of different thicknesses without splashing liquid.

[0007] Preferably, the circumferential surface of the rotating disc is provided with a plurality of aperture marks at corresponding positions of a plurality of gap holes, a plurality of positioning plates are arranged on the circumferential surface of the rotating disc between the plurality of aperture marks, the outer surface of the side pipe is a transparent part near the position of the air outlet hole, when one of the gap holes coincides with the air outlet hole, the corresponding aperture mark is located at the position of the transparent part, the aperture of the gap hole can be directly observed, the air outlet pressure can be known, and the precision is higher.

[0008] Preferably, a vertical shaft is arranged at the middle position of the bottom end of the rotating disc, one side of the bottom of the vertical shaft is connected with a horizontal shaft through a bevel gear, one end of the horizontal shaft extends out of the side pipe, and a plurality of anti-skid grooves are arranged on the extended end of the horizontal shaft, so that the horizontal shaft can be rotated conveniently, and when the horizontal shaft is driven to rotate, the vertical shaft is driven to rotate, and the rotating disc is rotated.

[0009] Preferably, a positioning disc is arranged on the circumferential surface of the bottom of the vertical shaft, a plurality of spring buttons are arranged on the circumferential surface of the positioning disc at corresponding positions of gap holes, and one side of the side pipe is matched with the spring buttons; the spring buttons are used in cooperation with the side pipe, so that the rotating disc is limited, and movement of the rotating disc when the gap hole coincides with the air outlet hole is prevented.

[0010] Preferably, one end of the side pipe is provided with a connecting section, and the outer surface of the connecting section is a threaded section, so that the side pipe can be connected with an air pump or other equipment, the connection mode is simple, and the sealing property is high.

[0011] Compared with the prior art, the utility model has the advantages that:

[0012] 1. The optimized blastocyst free DNA extraction assembly, by the clamping block at the top of the adjuster and the clamping groove arranged in the connecting piece, the diameter of the top of the adjuster is adjusted by the nut, the clamping block is connected with the clamping groove, the connecting piece and the connecting pipe are connected together by the adjuster, and when the operating arm of the micro-operation system operates the biopsy needle, the connecting pipe does not need to be manually held, and the stability is guaranteed.

[0013] 2. The optimized blastocyst free DNA extraction assembly, by the air outlet hole of the top disc and the gap hole with different apertures of the rotating disc, when gas is conveyed through the side pipe, the gap hole with different apertures adjusts the air outlet of the side pipe, and then the air outlet pressure is adjusted to blow away mineral oil with different thicknesses, and liquid splashing is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a whole structure schematic view of the utility model;

[0015] Figure 2 It is a hollow pipe structure part sectional view schematic view of the utility model;

[0016] Figure 3The utility model discloses a connecting piece structure part cross section schematic view.

[0017] Figure 4 The utility model discloses a side pipe structure part cross section schematic view.

[0018] Figure 5 The utility model discloses a top disc and carousel structure schematic view.

[0019] In the drawing: 1, hollow pipe; 2, regulator; 3, connecting piece; 4, nut; 5, side pipe; 6, connecting pipe mouth; 7, biopsy needle; 8, clamping groove; 9, clamping block; 10, clearance hole; 11, spring button; 12, cross axle; 13, positioning disc; 14, vertical axle; 15, carousel; 16, top disc; 17, air outlet; 18, aperture mark; 19, positioning plate; 20, connecting section. DETAILED DESCRIPTION

[0020] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model, and apparently, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative work belong to the range of protection of the utility model.

[0021] In the description of the utility model, it is necessary to explain that the orientation or position relation indicated by the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "another end" and the like is the orientation or position relation shown in the drawing, and is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore, it cannot be understood as the limitation of the utility model. In addition, the terms "first", "second" are only for the description purpose, and cannot be understood as indicating or implying relative importance.

[0022] As Figures 1 to 5As shown, the optimized blastocyst free DNA extraction assembly of the embodiment comprises a hollow tube 1, one side of the hollow tube 1 is provided with a side tube 5, the top end of the hollow tube 1 is provided with a connecting pipe 6, the inside of the hollow tube 1 is provided with a biopsy needle 7, the top of the biopsy needle 7 is connected with the operation arm of the micro-operation system, the needle tip of the biopsy needle 7 is inserted into the inside of the hollow tube 1, the biopsy needle 7 can be freely telescopic in the hollow tube 1, the top end of the connecting pipe 6 is annularly arranged with a plurality of adjusters 2, the outer surface of the plurality of adjusters 2 is threadedly connected with a nut 4, when the nut 4 is rotated downward from the top of the adjuster 2, the top of the plurality of adjusters 2 will be driven to shrink, the top end of the plurality of adjusters 2 is provided with a clamping block 9, the top end of the plurality of adjusters 2 is detachably mounted with a connecting piece 3, the top of the connecting piece 3 is connected with the outside of the operation arm of the micro-operation system, when the nut 4 is rotated downward from the adjuster 2, the top of the plurality of adjusters 2 shrinks until the circumferential surface of the clamping block 9 matches the inner wall of the connecting piece 3, the connecting piece 3 can be inserted into the top of the adjuster 2, the inside of the connecting piece 3 is provided with a clamping groove 8 matched with the clamping block 9, when the adjuster 2 moves to the clamping groove 8 at the top of the connecting piece 3, the top end of the clamping block 9 is blocked by the protrusion of the inner wall of the connecting piece 3, then the nut 4 is rotated in the opposite direction, the top of the plurality of adjusters 2 is expanded, the clamping block 9 is inserted into the inside of the clamping groove 8, the connecting piece 3 is connected with the adjuster 2, the connecting pipe 6 is conveniently mounted on the outside of the operation arm of the micro-operation system, when the operation arm of the micro-operation system operates the biopsy needle 7, the connecting pipe 6 does not need to be manually held, the stability is guaranteed, the inside of the side tube 5 is provided with a top disc 16, the inside of the bottom end of the top disc 16 is provided with a rotating disc 15, the top of one side of the top disc 16 is provided with an air outlet hole 17, the top of the rotating disc 15 is annularly arranged with a plurality of gap holes 10, the plurality of gap holes 10 can be driven by the rotating disc 15 to coincide with the air outlet hole 17 respectively, the air outlet of the side tube 5 is adjusted through the gap holes 10 with different hole diameters, and then the air pressure at the bottom of the hollow tube 1 is controlled.

[0023] Specifically, the plurality of gap holes 10 are circular holes with different hole diameters, the plurality of gap holes 10 are rotated by the rotating disc 15 to coincide with the air outlet hole 17 respectively, the air outlet of the side tube 5 is adjusted through the gap holes 10 with different hole diameters used in cooperation with the air outlet hole 17, and then the air outlet pressure is adjusted to blow off mineral oil with different thicknesses without splashing liquid.

[0024] Further, a plurality of hole diameter marks 18 are arranged at positions corresponding to the plurality of gap holes 10 on the circumferential surface of the rotating disc 15, a plurality of positioning plates 19 are arranged on the circumferential surface of the rotating disc 15 between the plurality of hole diameter marks 18, the outer surface of the side tube 5 is transparent at a position close to the air outlet hole 17, when one of the gap holes 10 coincides with the air outlet hole 17, the corresponding hole diameter mark 18 is located at the position of the transparent part, the hole diameter of the gap hole 10 can be directly observed, the air outlet pressure can be known, and the accuracy is higher.

[0025] Further, the vertical shaft 14 is arranged at the middle position of the bottom end of the rotating disc 15, and the bottom side of the vertical shaft 14 is connected with the horizontal shaft 12 through the oblique gear engagement, and one end of the horizontal shaft 12 extends out of the side pipe 5, and the extended end of the horizontal shaft 12 is provided with a plurality of anti-skid grooves, which facilitates the rotation of the horizontal shaft 12, and when the horizontal shaft 12 is driven to rotate, the vertical shaft 14 is driven to rotate, so as to rotate the rotating disc 15, and in actual use, the motor or manual work can be used as the power source.

[0026] Further, the circumferential surface of the bottom of the vertical shaft 14 is provided with the positioning disc 13, a plurality of spring buttons 11 are arranged at the corresponding positions of the circumferential surface gap holes 10 of the positioning disc 13, and one side of the side pipe 5 is matched with the spring buttons 11, and the spring buttons 11 are used in cooperation with the measuring pipe, so that the rotating disc 15 is limited, and it is ensured that the gap hole 10 and the air outlet hole 17 will not move when they are coincided.

[0027] Further, one end of the side pipe 5 is provided with the connecting section 20, and the outer surface of the connecting section 20 is a threaded section, so that the measuring pipe can be connected with the air pump or other equipment, and the connection mode is simple and has high sealing performance.

[0028] The use method of the embodiment is as follows: when the optimized blastocyst free DNA extraction assembly is used, the biopsy needle 7 is inserted into the hollow pipe 1, and then the nut 4 is rotated downward from the top of the adjuster 2, at this time, the top of the plurality of adjusters 2 is driven to contract, when the nut 4 is rotated downward from the top of the adjuster 2, the top of the plurality of adjusters 2 is contracted until the circumferential surface of the clamping block 9 is matched with the inner wall of the connecting piece 3, then the bottom of the connecting piece 3 connected with the operation arm outside of the micro-operation system is inserted into the top of the adjuster 2, when the adjuster 2 moves to the clamping groove 8 at the top of the connecting piece 3, the top end of the clamping block 9 is blocked by the protrusion of the inner wall of the connecting piece 3, then the nut 4 is rotated in the opposite direction, so that the top of the plurality of adjusters 2 is expanded, the clamping block 9 is inserted into the clamping groove 8, so that the connecting piece 3 is connected with the adjuster 2, which is convenient for installing the connecting pipe opening 6 outside the operation arm of the micro-operation system, so that when the operation arm of the micro-operation system operates the biopsy needle 7, the connecting pipe opening 6 does not need to be manually held, which ensures the stability, then the gas is input into the hollow pipe 1 through the side pipe 5, so that the mineral oil on the surface of the culture solution at the insertion position of the hollow pipe 1 is blown away, at this time, the biopsy needle 7 is extended, the biopsy needle 7 is inserted into the blastocyst from the incision on the blastocyst zona pellucida, the cells are sucked into the biopsy needle 7 by using negative pressure, the biopsy needle 7 is extracted from the blastocyst, the sucked cells are released for subsequent detection, and when the air is transported, the horizontal shaft 12 drives the vertical shaft 14 to rotate, so that the rotating disc 15 rotates, so that the corresponding gap hole 10 is coincided with the air outlet hole 17, the air outlet of the side pipe 5 is adjusted through the gap hole 10 with different hole diameters, and then the air pressure at the bottom of the hollow pipe 1 is controlled.

[0029] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for the equivalent replacement of part of the technical features of the technical solutions recorded in the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application, shall be included within the scope of the present application.

Claims

1. An optimized blastocyst free DNA extraction assembly comprising a hollow tube (1) characterized by: One side of the hollow pipe (1) is provided with a side pipe (5), the top end of the hollow pipe (1) is provided with a connecting pipe (6), the inside of the hollow pipe (1) is provided with a biopsy needle (7), the top end of the connecting pipe (6) is annularly provided with a plurality of adjusters (2), the outer surface of the plurality of adjusters (2) is threadedly connected with a nut (4), the top end of the plurality of adjusters (2) is provided with a clamping block (9), the top end of the plurality of adjusters (2) is detachably installed with a connecting piece (3), the inside of the connecting piece (3) is provided with a clamping groove (8) matched with the clamping block (9), the inside of the side pipe (5) is provided with a top disc (16), the inside of the bottom end of the top disc (16) is provided with a rotating disc (15), the top side of the top disc (16) is throughly provided with an air outlet hole (17), the top of the rotating disc (15) is annularly provided with a plurality of gap holes (10).

2. The optimized blastocyst EEV DNA extraction assembly of claim 1, wherein: The plurality of gap holes (10) are circular holes with different hole diameters.

3. The optimized blastocyst EEV DNA extraction assembly of claim 1, wherein: The circumferential surface of the rotating disc (15) is provided with a plurality of hole diameter marks (18) at positions corresponding to the plurality of gap holes (10), and the circumferential surface of the rotating disc (15) between the plurality of hole diameter marks (18) is provided with a plurality of positioning plates (19).

4. The optimized blastocyst EEV DNA extraction assembly of claim 1, wherein: The bottom end of the rotating disc (15) is provided with a vertical shaft (14) at the middle position, the bottom side of the vertical shaft (14) is connected with a horizontal shaft (12) through a helical gear, and one end of the horizontal shaft (12) extends out of the side pipe (5).

5. The optimized blastocyst EEV DNA extraction assembly of claim 4, wherein: The bottom circumferential surface of the vertical shaft (14) is provided with a positioning disc (13), the circumferential surface of the positioning disc (13) is provided with a plurality of spring buttons (11) at positions corresponding to the gap holes (10), and one side of the side pipe (5) is matched with the spring buttons (11).

6. The optimized blastocyst free DNA extraction assembly of claim 1, wherein: One end of the side pipe (5) is provided with a connecting section (20).

Images