Sampler for separating and extracting immune cells

Abstract

The utility model relates to the technical field of samplers, and discloses a sampler for separating and extracting immune cells, which comprises a needle cylinder, a connecting pipe is fixedly connected to the bottom of the needle cylinder, a needle tube is inserted into the surface of the connecting pipe, an installation mechanism is arranged on the surface of the needle cylinder, a positioning mechanism is arranged in the installation mechanism, and the installation mechanism comprises a cover body. The cover body is attached to the top of the needle cylinder, and the top of the cover body is fixedly connected with a first fixing plate. When the push rod is located at the initial position or in the using state, the stable locking function is provided, the elastic force of the spring pushes the insertion rod to be leftwards inserted into the insertion hole of the fixing block, the cover body and the needle cylinder are stably connected together, and when the parts need to be detached, cleaned or replaced, the pulling plate is pulled to overcome the elastic force of the spring, and the needle cylinder is replaced. By means of the locking mechanism, firmness in the using process is guaranteed, disassembly and maintenance are convenient, and operation is easy and convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of sampler, concretely is a sampler of immune cell separation and extraction. BACKGROUND

[0002] In today's biomedical field, the research and application of immune cells are showing a vigorous development trend. Immune cells not only play a key role in the process of human body resisting diseases, but also have irreplaceable value in disease diagnosis, treatment and drug development. For example, in tumor immunotherapy, by separating and extracting the immune cells of patients, after in vitro expansion and modification, the immune cells are returned to the patients, which can enhance the recognition and killing ability of the immune system to tumor cells, bringing new treatment hope for cancer patients. In the research of autoimmune diseases, accurate analysis of the function and quantity of immune cells helps to understand the pathogenesis of the disease, so as to develop more effective treatment strategies.

[0003] According to the announcement number CN 211689058 U, a cell cultivation sampler, including sleeve, the top of sleeve is connected with fixed plate, the bottom of fixed plate is fixedly installed with lug, the bottom of fixed plate is attached with glass tube, the top of glass tube is equally provided with groove, the lug is connected with groove, the bottom of sleeve is connected with support plate, the top of support plate and the inner cavity of sleeve are provided with channel.

[0004] The device solves the problem that the sampler is needed for sampling before cell cultivation, the existing cell sampling is performed by using tweezers, this sampling method is easy to make the cells slip, the sampling efficiency is low, and dust is easy to fall on the surface of the tweezers, causing bacterial infection of the cells. But the device cannot disassemble the fixed plate and the sleeve, so it is not convenient to disassemble and clean the parts or replace the parts. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a sampler for immune cell separation and extraction to solve the problems in the above background technology.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following technical scheme: a sampler for immune cell separation and extraction, including needle cylinder, the bottom of needle cylinder is fixedly connected with connecting pipe, the surface of connecting pipe is inserted with needle tube, the surface of needle cylinder is provided with mounting mechanism, the mounting mechanism is provided with positioning mechanism;

[0007] The mounting mechanism comprises a cover body which is attached to the top of the needle cylinder, a first fixed plate is fixedly connected to the top of the cover body, a push rod is arranged on the top of the first fixed plate, a push plate is fixedly connected to the top end of the push rod, a piston is fixedly connected to the bottom end of the push rod, a sealing groove is arranged on the surface of the piston, a sealing ring is sleeved in the sealing groove, a second fixed plate is fixedly connected to the bottom right end of the first fixed plate, plug rods are symmetrically arranged on the right side of the second fixed plate, pull plates are fixedly connected to the right ends of the plug rods, limit rings are fixedly connected to the surfaces of the plug rods close to the right ends, fixed rings are fixedly connected to the surfaces of the plug rods close to the left ends, springs are sleeved between the left side of the second fixed plate and the fixed rings on the surfaces of the plug rods, and fixed blocks are fixedly connected to the surface of the needle cylinder close to the top.

[0008] Preferably, holes matched with the push rod are arranged on the top of the cover body and the top of the first fixed plate, and the surface of the push rod penetrates and is connected to the holes in an up-down sliding mode.

[0009] Preferably, the outer wall of the sealing ring is connected to the inner wall of the needle cylinder in an up-down sliding mode, holes matched with the plug rods are arranged on the right side of the second fixed plate close to the bottom, and the surface of the plug rod penetrates and is connected to the holes in a left-right sliding mode.

[0010] Preferably, the right end of the spring is fixedly connected to the left side of the second fixed plate, the left end of the spring is fixedly connected to the right side of the fixed ring, the left end of the push rod is in a bevel shape, and the top of the fixed block is in a bevel shape on both ends and corresponds to the bevel shape of the left end of the plug rod.

[0011] Preferably, the second fixed plate, the plug rod, the pull plate, the limit ring, the fixed ring, the spring and the plug hole have two groups and are symmetrically arranged on both ends of the bottom of the first fixed plate.

[0012] Preferably, the positioning mechanism comprises a third fixed plate which is fixedly connected to the front and back sides of the first fixed plate in a symmetrical mode, a positioning block is fixedly connected to the bottom of the third fixed plate, fourth fixed plates are fixedly connected to the front and back sides of the fixed block in a symmetrical mode, and positioning grooves corresponding to the positioning block are arranged on the top of the fourth fixed plate.

[0013] Preferably, the bottom end of the positioning block is inserted into the positioning groove.

[0014] Compared with the prior art, the sampler for immune cell separation and extraction has the following beneficial effects:

[0015] The sampler for immune cell separation and extraction is provided with two sets of symmetrically arranged inserting rods, springs and other components, which provide stable locking function when the push rod is in the initial position or in use, the spring pushes the inserting rod to insert into the insertion hole of the fixed block, and the cover body and the needle cylinder are stably connected together, when it is needed to disassemble, clean or replace the components, the spring elastic force is overcome by pulling the pull plate, the inserting rod is pulled out of the insertion hole, and the cover body and the needle cylinder are easily separated, the locking mechanism ensures the firmness in use and facilitates disassembly and maintenance, and the operation is simple and convenient.

[0016] The sampler for immune cell separation and extraction is provided with two sets of symmetrically arranged inserting rods, springs and other components, which provide stable locking function when the push rod is in the initial position or in use, the spring pushes the inserting rod to insert into the insertion hole of the fixed block, and the cover body and the needle cylinder are stably connected together, when it is needed to disassemble, clean or replace the components, the spring elastic force is overcome by pulling the pull plate, the inserting rod is pulled out of the insertion hole, and the cover body and the needle cylinder are easily separated, the locking mechanism ensures the firmness in use and facilitates disassembly and maintenance, and the operation is simple and convenient. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings needed in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0018] Figure 1 It is a three-dimensional schematic view of the structure of the present application;

[0019] Figure 2 It is a three-dimensional schematic view of the structure of the present application;

[0020] Figure 3 It is a three-dimensional schematic view of the structure of the present application;

[0021] Figure 4 It is a three-dimensional schematic view of the structure of the present application.

[0022] In the figure: 1, needle cylinder; 2, connecting pipe; 3, needle pipe; 4, mounting mechanism; 41, cover body; 42, first fixed plate; 43, push rod; 44, push plate; 45, piston; 46, sealing groove; 47, sealing ring; 48, second fixed plate; 49, inserting rod; 411, pull plate; 412, limiting ring; 413, fixed ring; 414, spring; 415, fixed block; 416, insertion hole; 5, positioning mechanism; 51, third fixed plate; 52, positioning block; 53, fourth fixed plate; 54, positioning groove. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be clearly and completely described in connection with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] In the utility model, unless explicitly defined and limited, the terms such as "mounting", "connection", "connecting", "fixing" should be understood broadly, for example, can be fixedly connected, can be detachably connected, or integrated; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, can be the communication inside two elements or the interaction relationship of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0025] The utility model provides the following technical scheme: Embodiment 1

[0026] Please refer to Figures 1-3 The utility model provides a kind of technical scheme: a sampler for immune cell separation and extraction, including needle cylinder 1, needle cylinder 1 bottom is fixedly connected with connecting pipe 2, connecting pipe 2 surface is inserted with needle tube 3, needle cylinder 1 surface is provided with mounting mechanism 4, and mounting mechanism 4 is provided with positioning mechanism 5 in it;

[0027] Mounting mechanism 4 includes cover 41, cover 41 is attached with needle cylinder 1 top, cover 41 top is fixedly connected with first fixed plate 42, and first fixed plate 42 top is provided with push rod 43, and push rod 43 top end is fixedly connected with push plate 44, and push rod 43 bottom end is fixedly connected with piston 45, and piston 45 surface is provided with sealing groove 46, and sealing groove 46 is sleeved with sealing ring 47, and first fixed plate 42 bottom right end is fixedly connected with second fixed plate 48, and second fixed plate 48 right side is provided with insertion rod 49 symmetrically in front and back, and insertion rod 49 right end is fixedly connected with pull plate 411, and insertion rod 49 surface is fixedly connected with limit ring 412 close to right end, and insertion rod 49 surface is fixedly connected with fixed ring 413 close to left end, and spring 414 is sleeved between the left side of second fixed plate 48 and fixed ring 413 on the surface of insertion rod 49, and needle cylinder 1 surface is fixedly connected with fixed block 415 close to top, and fixed block 415 right side is provided with insertion hole 416 corresponding with the left end of insertion rod 49.

[0028] Cover 41 top and first fixed plate 42 top are provided with the hole matched with push rod 43, and push rod 43 surface is connected in the hole by penetrating and sliding up and down.

[0029] The outer wall of the sealing ring 47 is slidably connected to the inner wall of the needle cylinder 1. A hole matching the insertion rod 49 is formed in the right side of the second fixed plate 48 near the bottom, and the insertion rod 49 penetrates the hole and is slidably connected to the hole.

[0030] The right end of the spring 414 is fixedly connected to the left side of the second fixed plate 48, the left end of the spring 414 is fixedly connected to the right side of the fixed ring 413, the left end of the push rod 43 is in a beveled shape, the top of the fixed block 415 is in a beveled shape on both sides, and corresponds to the beveled shape of the left end of the insertion rod 49.

[0031] The second fixed plate 48, the insertion rod 49, the pull plate 411, the limiting ring 412, the fixed ring 413, the spring 414, and the insertion hole 416 have two groups and are symmetrically arranged on both ends of the bottom of the first fixed plate 42. Embodiment 2

[0032] Please refer to Figure 4 On the basis of Embodiment 1, the positioning mechanism 5 is further obtained.

[0033] The positioning mechanism 5 comprises a third fixed plate 51, the third fixed plate 51 is symmetrically fixedly connected to the front and back sides of the first fixed plate 42, the bottom of the third fixed plate 51 is fixedly connected with a positioning block 52, the front and back sides of the fixed block 415 are symmetrically fixedly connected with a fourth fixed plate 53, and the top of the fourth fixed plate 53 is provided with a positioning groove 54 corresponding to the positioning block 52.

[0034] The bottom end of the positioning block 52 is inserted into the positioning groove 54.

[0035] In actual operation, when the device is used, the bottom of the needle cylinder 1 is inserted into the needle tube 3 through the fixedly connected connecting tube 2. This connection mode can ensure the close connection between the needle tube 3 and the needle cylinder 1 during sample collection, preventing sample leakage. When sample collection is needed, the needle tube 3 is pierced into the target site, such as a vein for collecting a blood sample or a tissue site for collecting a tissue sample. The up-and-down movement of the push rod 43 is controlled by the push plate 44. The push rod 43 is fixedly connected to the push plate 44 at the top end and to the piston 45 at the bottom end. The push rod 43 can slide up and down in the matching holes formed in the top of the cover 41 and the first fixed plate 42. When the push plate 44 is pulled upward, the push rod 43 drives the piston 45 to move upward, forming a negative pressure in the needle cylinder 1. Since the outer wall of the sealing ring 47 closely fits the inner wall of the needle cylinder 1, the sealing of the needle cylinder 1 is ensured, so that the sample from the outside can be sucked into the needle cylinder 1 through the needle tube 3 and the connecting tube 2, completing the sample collection. When the cover 41 needs to be installed on the needle cylinder 1, the cover 41 is first attached to the top of the needle cylinder 1. At this time, due to the elastic force of the spring 414, the insertion rod 49 extends to the left. The right end of the insertion rod 49 is fixedly connected to the pull plate 411, facilitating the operator to pull the insertion rod 49. The limit ring 412 is fixedly connected to the surface of the insertion rod 49 near the right end, preventing the insertion rod 49 from moving too far to the right and disengaging from the second fixed plate 48. The fixed ring 413 is used to fix the left end of the spring 414. The right end of the spring 414 is fixedly connected to the left side of the second fixed plate 48. As the cover 41 gradually falls, the inclined surface on the top of the fixed block 415 interacts with the inclined surface on the left end of the insertion rod 49. Due to the guiding effect of the inclined surface, the insertion rod 49 is subjected to a rightward extrusion force and moves to the right against the elastic force of the spring 414. When the insertion hole 416 on the fixed block 415 is aligned with the left end of the insertion rod 49, the insertion rod 49 quickly inserts into the insertion hole 416 under the elastic force of the spring 414, thereby firmly fixing the cover 41 on the top of the needle cylinder 1. Moreover, since the second fixed plate 48, the insertion rod 49, and other components have two groups and are symmetrically arranged on the left and right ends of the bottom of the first fixed plate 42, the connection between the cover 41 and the needle cylinder 1 is more stable. When the cover 41 needs to be removed, the operator pulls the pull plates 411 on both sides simultaneously, causing the insertion rod 49 to move to the right against the elastic force of the spring 414 and disengage from the insertion hole 416. At this time, the cover 41 can be removed from the needle cylinder 1, facilitating the cleaning, maintenance, or replacement of the needle cylinder 1, the piston 45, and other components.

[0036] In the process of installing the cover 41, the positioning mechanism 5 plays an important role. The third fixed plate 51 is symmetrically fixed to the front and rear sides of the first fixed plate 42, and the positioning block 52 fixed to the bottom of the third fixed plate 51 is symmetrically fixed to the top of the fourth fixed plate 53 fixed to the front and rear sides of the fixed block 415. When the cover 41 approaches the top of the needle cylinder 1, the positioning block 52 will be aligned with the positioning groove 54. Since the shape and position of the positioning block 52 and the positioning groove 54 are accurately matched, the positioning block 52 will be accurately inserted into the positioning groove 54 under the guidance of gravity and the installation process. This positioning method ensures that the relative positions of the components remain consistent when the cover 41 is installed on the needle cylinder 1, whether it is the first installation or after multiple disassembly and reinstallation. It can ensure the accurate relative position of the push rod 43, the piston 45 and other components with the needle cylinder 1, thereby ensuring the stability and accuracy of the sampler during operation. For example, only accurate positioning can ensure smooth movement of the piston 45 in the needle cylinder 1, accurate docking of the insertion rod 49 with the insertion hole 416, and thus ensure the reliability and consistency of the entire sampling process.

[0037] It should be noted that the relational terms herein, such as first and second, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any such actual relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without more limitations, an element defined by the statement "comprising a..." does not exclude the existence of additional identical elements in the process, method, article, or apparatus including the element.

Claims

1. An immune cell separation extractor sampler comprising a syringe (1), characterized in that: The needle cylinder (1) bottom fixedly connected with connecting pipe (2), the connecting pipe (2) surface inserts needle tube (3), the needle cylinder (1) surface is provided with mounting mechanism (4), the mounting mechanism (4) is provided with positioning mechanism (5) in, The mounting mechanism (4) includes a cover (41), the cover (41) is attached to the top of the needle cylinder (1), the cover (41) top fixedly connected with first fixed plate (42), the first fixed plate (42) top is provided with push rod (43), the push rod (43) top fixedly connected with push plate (44), the push rod (43) bottom fixedly connected with piston (45), the piston (45) surface is provided with sealing groove (46), the sealing groove (46) is sleeved with sealing ring (47), the first fixed plate (42) bottom right end fixedly connected with second fixed plate (48), the second fixed plate (48) right side is provided with plug rod (49) symmetrically, the plug rod (49) right end fixedly connected with pull plate (411), the plug rod (49) surface is fixedly connected with limit ring (412) near the right end, the plug rod (49) surface is fixedly connected with fixed ring (413) near the left end, the plug rod (49) surface is sleeved with spring (414) between the second fixed plate (48) left side and the fixed ring (413), the needle cylinder (1) surface is fixedly connected with fixed block (415) near the top, the fixed block (415) right side is provided with insertion hole (416) corresponding to the left end of the plug rod (49).

2. The sampler for immune cell separation and extraction according to claim 1, wherein: The cover (41) top and the first fixed plate (42) top are provided with holes matched with the push rod (43), and the push rod (43) surface penetrates and is connected to the holes.

3. The sampler for immune cell separation and extraction according to claim 1, wherein: The sealing ring (47) outer wall is connected to the inner wall of the needle cylinder (1) up and down, the second fixed plate (48) right side is provided with a hole matched with the plug rod (49) near the bottom, and the plug rod (49) surface penetrates and is connected to the hole left and right.

4. The sampler for immune cell separation and extraction according to claim 1, wherein: The spring (414) right end is fixedly connected to the left side of the second fixed plate (48), the spring (414) left end is fixedly connected to the right side of the fixed ring (413), the left end of the push rod (43) is inclined, and the top of the fixed block (415) is inclined on both ends, and corresponds to the inclined shape of the left end of the plug rod (49).

5. The sampler for immune cell separation and extraction according to claim 1, wherein: The second fixed plate (48), plug rod (49), pull plate (411), limit ring (412), fixed ring (413), spring (414) and insertion hole (416) have two groups, and are symmetrically arranged on both ends of the first fixed plate (42) bottom.

6. The sampler for immune cell separation and extraction according to claim 1, wherein: The positioning mechanism (5) includes a third fixed plate (51), the third fixed plate (51) is fixedly connected to the front and back of the first fixed plate (42), the third fixed plate (51) bottom fixedly connected with positioning block (52), the fixed block (415) front and back symmetrically fixedly connected with fourth fixed plate (53), the fourth fixed plate (53) top is provided with positioning groove (54) corresponding to the positioning block (52).

7. The sampler for immune cell separation and extraction according to claim 6, wherein: The positioning block (52) is inserted into the positioning groove (54) at the bottom end.

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