Intelligent temperature control and regulation conversion device for producing trehalose

Abstract

The utility model provides an intelligent temperature control regulation conversion device for trehalose production, which is characterized in that N groups of heat exchange coils are arranged on the inner wall of a conversion tank body, stirring pieces comprise a first group of stirring pieces and a second group of stirring pieces, the first group of stirring pieces comprises M first stirring units which are uniformly arranged along the length direction of a stirring shaft, and the second group of stirring pieces comprises M second stirring units which are uniformly arranged along the length direction of the stirring shaft; the second group of stirring pieces comprises M-1 second stirring units, each second stirring unit is arranged between two adjacent first stirring units, and an angle of 180 degrees is formed between each second stirring unit and the corresponding first stirring unit. A first temperature sensor, a second temperature sensor and a third temperature sensor are further arranged, the first temperature sensor and the second temperature sensor are arranged on the first stirring unit located at the top end and the first stirring unit located at the bottom end respectively, and the third temperature sensor is arranged in the middle of the stirring shaft in the length direction. The multiple groups of heat exchange coils are arranged on the inner wall of the conversion tank and matched with the temperature sensors arranged at multiple positions in the tank body, so that the temperature change of a solution in the tank is detected in real time, and the temperature in the tank is flexibly controlled.

Description

TECHNICAL FIELD

[0001] The utility model relates to trehalose production equipment technical field, concretely relates to a kind of intelligent temperature control adjustment's trehalose production conversion device. BACKGROUND

[0002] Trehalose, also known as mushroom sugar, is a non-reducing disaccharide composed of two glucose molecules through hemiacetal hydroxyl condensation. It is widely present in bacteria, yeast, fungi, algae and insects. Trehalose has unique biological functions, can protect proteins, biological membranes and sensitive cell walls from damage caused by drought, freezing and osmotic pressure changes, and can be used as a stabilizer for unstable drugs, food and cosmetics, a sweetener for various food and drugs, a seed coating, a protective agent for freeze-dried strains, etc. Trehalose can also protect DNA from radiation-induced damage. Currently, there are three main methods for preparing trehalose in laboratories and production: one is to extract it from yeast cells, but the extraction process is complex, separation and extraction are difficult, and the cost is high; the second is to produce it by microbial fermentation and extract it from the fermentation broth, which is difficult to extract due to the complex composition of the fermentation broth; the third is to extract related trehalose synthase by microbial fermentation, and to synthesize trehalose by enzyme transglycosylation. Enzymatic synthesis is the most promising method among various trehalose production methods so far, and its emergence has laid a solid foundation for large-scale industrial production of trehalose.

[0003] Trehalose production requires the introduction of prepared maltose solution into the conversion tank for conversion, the conversion temperature is maintained at 35℃, and the pH is maintained at about 7.0. Stirring reaction for 48h. Terminate the conversion, then heat to 80℃ and maintain for 10min to inactivate the enzyme. The enzyme is particularly sensitive to temperature during the conversion process. Currently, the conversion tank only uses a heat exchange jacket to heat the conversion tank, resulting in high temperature of the solution near the inner wall of the conversion tank and low temperature of the solution inside the conversion tank, which is not conducive to enzyme reaction. To solve the above problems, an intelligent temperature control adjustment trehalose production conversion device is proposed. UTILITY MODEL CONTENTS

[0004] The utility model aims at providing an intelligent temperature control adjustment trehalose production conversion device, which is equipped with multiple heat exchange tubes and multiple position thermometers inside the device, realizes multi-angle temperature measurement, and can regulate the temperature inside the device according to real-time temperature to maintain efficient enzyme conversion.

[0005] The utility model provides a kind of intelligent temperature control regulation's trehalose production with conversion device, including conversion tank and stirring assembly, the stirring assembly is located in the conversion tank inside.The conversion tank tank body inner wall is equipped with N group heat exchange coil pipe, N is the integer greater than 2, every group the heat exchange coil pipe includes a liquid inlet and a liquid outlet, the liquid inlet and the liquid outlet are connected with the pump being equipped in conversion tank outside communication.The stirring assembly includes motor, stirring shaft and stirring piece, the motor output end is connected with the stirring shaft, the stirring piece includes first group stirring piece and second group stirring piece, the first group stirring piece includes M first stirring unit, M is the integer greater than 4, the first stirring unit is evenly arranged along the length direction of the stirring shaft.The second group stirring piece includes M-1 second stirring unit, the second stirring unit is located in the intermediate of adjacent two first stirring units and second stirring unit and first stirring unit are 180 ° angle.The conversion tank is equipped with first temperature sensor, second temperature sensor and third temperature sensor, and the first temperature sensor and second temperature sensor are respectively equipped in the first stirring unit at top and the first stirring unit at bottom, and the third temperature sensor is equipped in the length direction of the stirring shaft middle.

[0006] Preferably, the first temperature sensor, second temperature sensor and third temperature sensor are electrically connected to a PLC controller, the motor and the pump are electrically connected to the PLC controller, the first temperature sensor, second temperature sensor and third temperature sensor detect the temperature in the conversion tank and generate an electrical signal to the PLC controller, the PLC controller receives the electrical signal to generate a control signal, and the control signal is transmitted to the motor or the pump to control the motor speed or control the start and stop of the pump.

[0007] Preferably, the conversion tank tank body outer wall is provided with a display, and the display is electrically connected to the first temperature sensor, second temperature sensor and third temperature sensor for displaying the temperature at different positions in the conversion tank.

[0008] Preferably, a filter screen is provided in the conversion tank, the filter screen is arranged below the stirring shaft and the heat exchange coil pipe, and the filter screen is inclinedly arranged at an angle of 45° with the conversion tank tank body inner wall.

[0009] Preferably, the filter screen is provided with a cleaning port near one end of the bottom of the conversion tank, and the cleaning port is arranged on the side wall of the conversion tank for cleaning the filter screen.

[0010] Preferably, the conversion tank is provided with a feed inlet at the top and a discharge outlet at the bottom, and the discharge outlet is arranged at the bottom of the filter screen.

[0011] Preferably, the inner wall of the conversion tank body is provided with four groups of heat exchange coils, which are a first heat exchange coil, a second heat exchange coil, a third heat exchange coil and a fourth heat exchange coil.

[0012] Preferably, the first heat exchange coil, the second heat exchange coil and the third heat exchange coil are connected to a hot medium for increasing the temperature in the conversion tank, and the fourth heat exchange coil is connected to a cold medium for reducing the temperature in the conversion tank.

[0013] Preferably, the first group of stirring units includes six first stirring units, and the second group of stirring units includes five second stirring units.

[0014] The utility model discloses a kind of intelligent temperature control adjustment's trehalose production conversion devices, by being provided with multiple groups of heat exchange coils in the inner wall of conversion tank, cooperate three temperature sensors arranged in the upper, middle and lower of tank body, real-time detection tank solution temperature variation, while cooperating with the hot medium or cold medium into heat exchange coil, heat exchange with tank solution, can make tank solution keep in reaction suitable temperature at moment, it is favorable to improve conversion rate.Multiple groups of heat exchange coils that do not affect each other are set, one or more groups can be selected to be used for cooling, and the other groups are used for heating.Because trehalose conversion needs to be kept at a suitable temperature, multiple solutions are needed for heating in the early stage, and multiple heat exchange coils for heating work at this time.As the temperature rises, the number of heat exchange coils for heating can be gradually reduced, so that the temperature rises steadily, and enzyme activity is prevented from decreasing due to difficult temperature control.As the reaction proceeds and frictional heat is generated by stirring, the temperature may occasionally exceed the suitable temperature range during the reaction.At this time, cold medium is introduced into the reserved heat exchange coil for cooling, and heat exchange mode is used to cool the tank solution.Each group of heat exchange coils works independently, which can effectively improve the flexibility of temperature control in the tank, and is beneficial to enzyme reaction.In order to further ensure the accuracy of tank solution temperature detection, three temperature sensors are used in the present application, which can monitor the temperature of multiple positions in the tank.When the temperature difference between the three temperature sensors exceeds a certain range, the motor speed can be increased to speed up stirring, the solution flow rate can be increased, heat exchange can be more uniform, and enzyme inactivation can be avoided to reduce conversion rate. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a structure diagram of the intelligent temperature control adjustment's trehalose production conversion device of the utility model.

[0016] Figure 2 It is a partial structure diagram of the conversion tank of the utility model.

[0017] In the figure: 1 - conversion tank, 2 - heat exchange coil, 3 - filter screen, 4 - cleaning port, 5 - first temperature sensor, 6 - feed port, 7 - motor, 9 - first stirring unit, 10 - second stirring unit, 11 - display, 12 - third temperature sensor, 13 - second temperature sensor, 14 - discharge port, 201 - liquid inlet, 202 - liquid outlet. DETAILED DESCRIPTION

[0018] In order to make the personnel in the technical field better understand the utility model scheme, the utility model is further described in detail below in combination with the drawings. The described embodiments are only a part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without making creative labor belong to the scope of protection of the utility model.

[0019] Reference Figure 1 and Figure 2The embodiment provides a smart temperature control and adjustment trehalose production conversion device, which comprises a conversion tank 1 and a stirring assembly arranged in the conversion tank 1. The inner wall of the conversion tank body is provided with N groups of heat exchange coils 2, N is an integer greater than 2, and N includes but is not limited to 3, 4, 5 and 6. Each group of heat exchange coils 2 comprises a liquid inlet 201 and a liquid outlet 202, the liquid inlet 201 and the liquid outlet 202 are communicated with a pump arranged outside the conversion tank 1, and the pump is used for conveying a hot medium or a cold medium to the heat exchange coil 2, and the hot medium or the cold medium flows out from the liquid inlet 201 and the liquid outlet 202. Each group of heat exchange coils 2 corresponds to a pump, and independent work is realized without affecting each other. The stirring assembly comprises a motor 7, a stirring shaft and stirring pieces, the output end of the motor 7 is connected with the stirring shaft, the stirring pieces comprise a first group of stirring pieces and a second group of stirring pieces, the first group of stirring pieces comprises M first stirring units 9, M is an integer greater than 4, and M includes but is not limited to 5, 6 and 7. The first stirring units 9 are uniformly arranged along the length direction of the stirring shaft. The second group of stirring pieces comprises M-1 second stirring units 10, the second stirring unit 10 is arranged between adjacent two first stirring units 9 and the second stirring unit 10 is at an angle of 180° with the first stirring unit 9. The first stirring unit 9 and the second stirring unit 10 are arranged in an alternating manner, and when rotating, the first stirring unit 9 and the second stirring unit 10 can produce stirring at different positions of the solution, improve the stirring efficiency of the solution in the tank body, and make the solution flow more strongly and easily produce mixed flow, so that heat exchange is more rapid. The conversion tank 1 is provided with a first temperature sensor 5, a second temperature sensor 13 and a third temperature sensor 12, the first temperature sensor 5 and the second temperature sensor 13 are arranged at the first stirring unit 9 at the top end and the first stirring unit 9 at the bottom end respectively, and the third temperature sensor 12 is arranged at the middle of the length direction of the stirring shaft. The first temperature sensor 5 and the second temperature sensor 13 are arranged at the upper and lower positions close to the heat exchange coil 2, and the third temperature sensor 12 is arranged at the central position in the tank, so that the temperatures of the solutions close to the tank wall and the tank center can be detected at the same time, the heat transfer condition of the solution in the tank is judged through the temperature difference, the temperature difference is the temperature difference value detected by the first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 two by two, the temperature difference detected by the first temperature sensor 5 and the second temperature sensor 13 is a first temperature difference, the temperature difference detected by the first temperature sensor 5 and the third temperature sensor 12 is a second temperature difference, and the temperature difference detected by the second temperature sensor 13 and the third temperature sensor 12 is a third temperature difference. When the first temperature difference or the second temperature difference or the third temperature difference exceeds a preset range, the rotating speed of the motor 7 can be adjusted to speed up the stirring speed and promote the flow speed of the solution, so as to maintain the overall stability of the solution temperature in the tank, prevent local overheating and reduce enzyme activity.

[0020] The conversion tank 1 is provided with a filter screen 3, which is arranged below the stirring shaft and the heat exchange coil 2 and is inclined at an angle of 45° with the inner wall of the tank body of the conversion tank 1.

[0021] The filter screen 3 is provided with a cleaning port 4 at one end close to the bottom of the conversion tank 1, which is arranged on the side wall of the conversion tank 1 for cleaning the filter screen 3.

[0022] The conversion tank 1 is provided with a feed port 6 at the top and a discharge port 14 at the bottom, which is arranged at the bottom of the filter screen 3.

[0023] Example 2

[0024] The difference between this embodiment and Example 1 is that:

[0025] The first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 are electrically connected to a PLC controller, and the motor 7 and the pump are also electrically connected to the PLC controller. The first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 detect the temperature in the conversion tank 1 and generate an electric signal which is transmitted to the PLC controller. The PLC controller receives the electric signal and generates a control signal which is transmitted to the motor 7 or the pump, so as to control the speed of the motor 7 or the start and stop of the pump. When the temperature difference of the first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 exceeds the preset range, an electric signal A is generated and transmitted to the PLC controller, and the PLC controller generates a control signal which is transmitted to the motor 7. The motor 7 receives the control signal and adjusts the speed of the motor 7, so as to adjust the stirring speed. When the temperature difference of the first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 is still within the preset range, but the temperature detection value of the first temperature sensor 5 or the second temperature sensor 13 or the third temperature sensor 12 is lower than the preset reaction temperature, an electric signal B is generated and transmitted to the PLC controller, and the PLC controller generates a control signal which is transmitted to the pump connected to the heat exchange coil of the hot medium. The pump is opened, and the hot medium flows into the heat exchange coil 2, so as to heat the solution. When the temperature difference of the first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 is still within the preset range, but the temperature detection value of the first temperature sensor 5 or the second temperature sensor 13 or the third temperature sensor 12 is higher than the preset reaction temperature, an electric signal C is generated and transmitted to the PLC controller, and the PLC controller generates a control signal which is transmitted to the pump connected to the heat exchange coil of the cold medium. The pump is opened, and the cold medium flows into the heat exchange coil 2, so as to cool the solution.

[0026] The display 11 is electrically connected with the first temperature sensor 5, the second temperature sensor 13 and the third temperature sensor 12 for displaying the temperatures at different positions in the conversion tank 1.

[0027] Embodiment 3

[0028] The embodiment differs from the embodiment 1 in that:

[0029] The inner wall of the conversion tank body is provided with four groups of heat exchange coils 2, namely a first heat exchange coil, a second heat exchange coil, a third heat exchange coil and a fourth heat exchange coil.

[0030] The first heat exchange coil 2, the second heat exchange coil 2 and the third heat exchange coil 2 are connected with a heat medium for increasing the temperature in the conversion tank 1, and the third heat exchange coil 2 is connected with a cold medium for decreasing the temperature in the conversion tank 1.

[0031] The first group of stirring members includes six first stirring units 9, and the second group of stirring members includes five second stirring units 10.

[0032] The utility model discloses a kind of intelligent temperature control adjustment's trehalose production conversion devices, set multiple groups of heat exchange coils, cooperate with being set in the temperature sensor of many places in tank body, real-time detection tank solution temperature variation, while cooperating with the heat medium or cold medium into heat exchange coil, with tank solution carries out heat exchange, can make tank solution keep in reaction suitable temperature moment, it is favorable to improve conversion rate.Temperature sensor detection temperature difference of each site can conversion tank solution temperature uniformity, can flexibly adjust stirring rate according to inspection temperature difference.

[0033] The above disclosed preferred embodiments of the utility model are only used to help explain the utility model, and do not limit the utility model to only the specific embodiments described. Obviously, according to the content of the specification, other modifications and changes can be made. The embodiments selected and specifically described in the specification are to better explain the principles and practical applications of the utility model, so that those skilled in the art can well understand and utilize the utility model, and are not a limitation on the utility model. Any simple modification of the utility model is within the protection scope of the utility model.

Claims

1. A smart temperature-controlled conversion device for trehalose production, comprising a conversion tank and a stirring assembly arranged in the conversion tank, characterized in that, The inner wall of the conversion tank body is provided with N groups of heat exchange coils, N is an integer greater than 2, each group of heat exchange coils comprises an inlet and an outlet, the inlet and the outlet are communicated with the pump arranged outside the conversion tank; the stirring assembly comprises a motor, a stirring shaft and a stirring part, the output end of the motor is connected with the stirring shaft, the stirring part comprises a first group of stirring parts and a second group of stirring parts, the first group of stirring parts comprises M first stirring units, M is an integer greater than 4, the first stirring units are uniformly arranged along the length direction of the stirring shaft; the second group of stirring parts comprises M-1 second stirring units, the second stirring unit is arranged between the adjacent two first stirring units and the second stirring unit is at an angle of 180° with the first stirring unit; the conversion tank is provided with a first temperature sensor, a second temperature sensor and a third temperature sensor, the first temperature sensor and the second temperature sensor are arranged at the first stirring unit at the top end and the first stirring unit at the bottom end respectively, and the third temperature sensor is arranged at the middle of the length direction of the stirring shaft.

2. The intelligent temperature-controlled conversion device for trehalose production according to claim 1, wherein, The first temperature sensor, the second temperature sensor and the third temperature sensor are electrically connected with a PLC controller, the motor and the pump are electrically connected with the PLC controller, the first temperature sensor, the second temperature sensor and the third temperature sensor detect the temperature in the conversion tank and generate an electric signal transmitted to the PLC controller, the PLC controller receives the electric signal to generate a control signal, and the control signal is transmitted to the motor or the pump to control the rotating speed of the motor or the start and stop of the pump.

3. The smart temperature-controlled conversion device for trehalose production according to claim 2, wherein, A display is arranged on the outer wall of the conversion tank body, the display is electrically connected with the first temperature sensor, the second temperature sensor and the third temperature sensor for displaying the temperature at different positions in the conversion tank.

4. The intelligent temperature-controlled conversion device for trehalose production according to claim 1, wherein, A filter screen is arranged in the conversion tank, the filter screen is arranged below the stirring shaft and the heat exchange coil, and the filter screen is arranged at an angle of 45° with the inner wall of the conversion tank body.

5. The smart temperature-controlled conversion device for trehalose production according to claim 4, wherein, A cleaning port is arranged at one end of the filter screen near the bottom of the conversion tank, and the cleaning port is arranged on the side wall of the conversion tank for cleaning the filter screen.

6. The smart temperature-controlled conversion device for trehalose production according to claim 4, wherein, A feeding port is arranged at the top of the conversion tank, and a discharging port is arranged at the bottom of the conversion tank, and the discharging port is arranged at the bottom of the filter screen.

7. The smart temperature-controlled conversion device for trehalose production according to claim 1, wherein, The inner wall of the conversion tank body is provided with 4 groups of heat exchange coils, which are first heat exchange coil, second heat exchange coil, third heat exchange coil and fourth heat exchange coil, and the first heat exchange coil, the second heat exchange coil, the third heat exchange coil and the fourth heat exchange coil are uniformly arranged on the inner wall of the conversion tank body.

8. The smart temperature-controlled conversion device for trehalose production according to claim 7, wherein, Hot medium is introduced into the first heat exchange coil, the second heat exchange coil and the third heat exchange coil to increase the temperature in the conversion tank, and cold medium is introduced into the third heat exchange coil to reduce the temperature in the conversion tank.

9. The smart temperature-controlled conversion device for trehalose production according to claim 1, wherein, The first group of stirring parts comprises 6 first stirring units, and the second group of stirring parts comprises 5 second stirring units.

Images