Multi-channel polypeptide solid-phase synthesis reaction device

Abstract

The utility model relates to a multichannel polypeptide solid-phase synthesis reaction device, and relates to the field of multichannel polypeptide solid-phase synthesis reaction instruments, the multichannel polypeptide solid-phase synthesis reaction device comprises a fixed table top on which a pipettor is mounted in a lifting adjustment manner, and a position detection unit mounted on a driving rod of an electric push rod on the fixed table top, the height sensor is used for detecting the height of a gun head of the pipettor and outputting a detection control signal when the gun head is moved out of a test tube; the liquid leakage detection unit is fixedly mounted on the inner wall of the cabinet body, is in signal connection with the signal output end of the position detection unit, and is used for receiving the detection control signal and outputting a first warning signal when liquid leakage occurs below the gun head of the pipettor; and the buzzer is fixedly mounted on the fixed table top, is in signal connection with the signal output end of the liquid leakage detection unit, and is used for receiving the first warning signal and making a warning sound. According to the invention, whether the pipettor of the multi-channel polypeptide solid-phase synthesis reaction device leaks liquid can be automatically detected.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of multi-channel polypeptide solid-phase synthesis reaction instrument, in particular to a multi-channel polypeptide solid-phase synthesis reaction device. BACKGROUND

[0002] In the development process of skin care products, it is often necessary to use a multi-channel polypeptide solid-phase synthesis reaction instrument for polypeptide solid-phase synthesis reaction. The multi-channel polypeptide solid-phase synthesis reaction instrument uses solid-phase synthesis as the reaction principle, and continuously adds, reacts, and synthesizes amino acids in a known order (sequence, generally from the C-terminal -carboxyl end to the N-terminal -amino end) in a closed explosion-proof glass reactor to finally obtain a polypeptide carrier. The solid-phase synthesis method greatly reduces the difficulty of purification of each product. Compared with a single-channel polypeptide synthesis instrument, the multi-channel polypeptide synthesis instrument can complete the synthesis of multiple polypeptides at the same time on the same set of equipment.

[0003] In the prior art, a polypeptide synthesis instrument with publication number CN111072749B is disclosed, which is a multi-channel polypeptide synthesis instrument. The instrument mainly includes a cabinet body, a fixed table is installed in the cabinet body, a pipette, a peristaltic pump, and a controller are installed on the fixed table, the addition of solvents is controlled by the peristaltic pump, a disc is rotatably installed in the cabinet body of the multi-channel polypeptide solid-phase synthesis reaction device, a test tube support is fixedly installed on the disc, and a rotating drive mechanism is installed at the bottom of the disc; a pipette is installed in the cabinet body and is arranged above the test tube support. The pipette can be driven to move up and down by the driving rod of the electric push rod, and the pipette can control the suction or output of liquid through the controller and the peristaltic pump.

[0004] However, the common multi-channel polypeptide solid-phase synthesis reaction device does not have a device for detecting whether the gun head of the pipette leaks, which is not convenient for timely maintenance and repair of the pipette of the multi-channel polypeptide solid-phase synthesis reaction device when the pipette leaks, and there is room for improvement. CONTENT OF THE INVENTION

[0005] In order to automatically detect whether the pipette of the multi-channel polypeptide solid-phase synthesis reaction device leaks, the present application provides a multi-channel polypeptide solid-phase synthesis reaction device.

[0006] The multi-channel polypeptide solid-phase synthesis reaction device provided by the present application adopts the following technical scheme:

[0007] A multi-channel polypeptide solid-phase synthesis reaction device mainly includes a fixed table, a pipette that can be adjusted to move up and down is installed on the fixed table, and the following:

[0008] A position detection unit is installed on the driving rod of the electric push rod on the fixed table, which is used to detect the height of the gun head of the pipette and output a detection control signal when the gun head is moved out of the test tube.

[0009] A liquid leakage detection unit is fixedly installed on the inner wall of the cabinet body and is in signal connection with the signal output end of the position detection unit, for receiving the detection control signal and outputting a first warning signal when liquid leakage occurs below the gun head of the pipettor;

[0010] A buzzer is fixedly installed on the fixed table top and is in signal connection with the signal output end of the liquid leakage detection unit, for receiving the first warning signal and emitting a warning sound.

[0011] By adopting the above technical scheme, in the process of the pipettor performing pipetting operation, the position detection unit detects the height of the gun head of the pipettor in real time, and outputs a detection control signal when the gun head is completely extended from the test tube, the liquid leakage detection unit detects whether there is liquid leakage below the gun head in real time, and controls the buzzer to emit a warning sound if there is liquid leakage; when the gun head is lowered to extend into the test tube, the position detection unit controls the liquid leakage detection unit to stop running. By mutual matching and use among the position detection unit, the liquid leakage detection unit and the buzzer, whether there is liquid leakage in the pipettor can be detected by detecting whether there is liquid (droplet) below the gun head when the gun head is moved out of the test tube, and if there is liquid leakage in the pipettor, the on-site operator can be prompted in time to repair and maintain the pipettor through the buzzer.

[0012] Preferably, a guide rod is fixedly installed at the bottom of the fixed table top, a connecting plate is slidingly installed on the guide rod, and the connecting plate is detachably connected with the driving rod; the position detection unit comprises:

[0013] An infrared distance measuring sensor is fixedly installed on the connecting plate, for detecting the distance between the connecting plate and the bottom surface of the fixed table top and outputting an inductive distance signal;

[0014] A first single-chip microcomputer is in signal connection with the signal output end of the infrared distance measuring sensor, for receiving the inductive distance and outputting the detection control signal when the inductive distance is less than a set value.

[0015] By adopting the above technical scheme, in the process of the driving rod driving the gun head of the pipettor to move up and down, the connecting plate and the infrared distance measuring sensor can be synchronously driven to move up and down, since the position of the gun head on the driving rod is fixed, the first single-chip microcomputer can calculate whether the gun head extends into or out of the test tube through comparison and calculation, and output the detection control signal when the gun head extends out of the test tube, so as to control the liquid leakage detection unit to detect the gun head of the pipettor for liquid leakage.

[0016] Preferably, the liquid leakage detection unit comprises:

[0017] The ultrasonic sensor is fixedly installed on the inner wall of the cabinet of the multi-channel polypeptide solid-phase synthesis reaction device, located between the gun head and the test tube support, and has an induction end facing the gun head, for detecting the liquid drops falling through the gun body and outputting a liquid leakage induction signal.

[0018] The second single-chip microcomputer is connected with the signal output end of the ultrasonic sensor, for receiving the liquid leakage induction signal and outputting the first warning signal.

[0019] By adopting the above technical scheme, the ultrasonic wave emitted by the ultrasonic transmitter in the ultrasonic sensor is received by the ultrasonic receiver in the ultrasonic sensor after contacting the liquid drops, and whether there is liquid leakage falling below the gun head can be determined by comparing the strength of the ultrasonic signal. If there is liquid leakage falling, the ultrasonic sensor outputs a liquid leakage induction signal, and the second single-chip microcomputer can output a first warning signal to control the buzzer to emit a warning sound.

[0020] Preferably, a mounting plate is fixedly installed on the side wall of the driving rod, two first through holes are formed through the mounting plate, two second through holes are formed through the connecting plate and matched with the two first through holes, and the mounting plate and the connecting plate are connected by two bolts.

[0021] By adopting the above technical scheme, the first through holes on the mounting plate and the second through holes on the connecting plate can facilitate the operator to connect the connecting plate and the mounting plate by two bolts.

[0022] Preferably, a mounting groove is formed in the connecting plate and located between the two second through holes.

[0023] A pressure sensor is embedded in the mounting groove, which detects the contact pressure between the mounting plate and the connecting plate and outputs a contact pressure signal.

[0024] The signal output end of the pressure sensor is connected with the signal output end of the second single-chip microcomputer, the second single-chip microcomputer receives the contact pressure signal and outputs a second warning signal when the contact pressure is greater than a set pressure value, and the buzzer receives the second warning signal and emits a warning sound.

[0025] By adopting the above technical scheme, during the operation of the multi-channel polypeptide solid-phase synthesis reaction device, the pressure sensor detects the contact pressure between the mounting plate and the connecting plate in real time. When the gun head collides with the underlying mechanism (test tube support, etc.) due to the abnormality of the electric push rod, the contact pressure detected by the pressure sensor is greater than the set pressure value, and the second single-chip microcomputer controls the buzzer to emit a warning sound.

[0026] In summary, the multi-channel polypeptide solid-phase synthesis reaction device has at least one of the following beneficial technical effects:

[0027] 1. In the process of driving the gun head of the pipette to rise and fall up and down by the driving rod, the connecting plate and the infrared distance sensor can be synchronously driven to rise and fall up and down. Since the position of the gun head on the driving rod is fixed, the first single-chip microcomputer can calculate whether the gun head is inserted into or withdrawn from the test tube through comparison and calculation, and output a detection control signal when the gun head is withdrawn from the test tube, so as to control the liquid leakage detection unit to detect the liquid leakage of the gun head of the pipette.

[0028] 2. The ultrasonic wave emitted by the ultrasonic wave transmitter in the ultrasonic wave sensor is received by the ultrasonic wave receiver in the ultrasonic wave sensor after contacting with the liquid drop. Whether there is liquid leakage dropping below the gun head can be judged by comparing the strength of the ultrasonic wave signal. If there is liquid leakage dropping, the ultrasonic wave sensor outputs a liquid leakage sensing signal, and the second single-chip microcomputer can output a first warning signal to control the buzzer to emit a warning sound. BRIEF DESCRIPTION OF DRAWINGS

[0029] Fig. 1 is a schematic diagram for showing the overall structure of the pipette according to Embodiment 1 of the present application.

[0030] Fig. 2 is a schematic diagram for showing the connecting structure between the connecting plate and the mounting plate according to Embodiment 1 of the present application.

[0031] Fig. 3 is a schematic diagram for showing the mounting position of the pressure sensor according to Embodiment 2 of the present application.

[0032] Explanation of reference numerals: 1, fixed table top; 11, guide rod; 12, connecting plate; 13, driving rod; 14, mounting plate; 15, first through hole; 16, second through hole; 17, bolt; 18, mounting groove; 2, pipette; 3, buzzer; 4, infrared distance sensor; 5, ultrasonic wave sensor; 6, pressure sensor. DETAILED DESCRIPTION

[0033] The following will be described in detail in combination with the accompanying Figs. 1-3 The present application will be further described in detail.

[0034] Embodiment 1

[0035] The present application discloses a kind of polypeptide solid-phase synthesis reaction device of multi-channel. Refer to Figs. 1-2The device mainly comprises a fixed table 1, a pipette 2 which is installed on the fixed table 1 and can be adjusted in lifting, and a position detection unit which is installed on the driving rod 13 of the electric push rod on the fixed table 1, is used for detecting the height of the gun head of the pipette 2 and outputting a detection control signal when the gun head is moved out of the test tube, a liquid leakage detection unit which is fixedly installed on the inner wall of the cabinet body, is connected with the signal output end of the position detection unit, is used for receiving the detection control signal and outputting a first warning signal when liquid leakage appears below the gun head of the pipette 2, and a buzzer 3 which is fixedly installed on the fixed table 1, is connected with the signal output end of the liquid leakage detection unit, is used for receiving the first warning signal and emitting a warning sound.

[0036] In the process that the pipette 2 of the multi-channel polypeptide solid-phase synthesis reaction device performs a pipetting operation, the position detection unit detects the height of the gun head of the pipette 2 in real time, and outputs a detection control signal when the gun head is completely extended out of the test tube, the liquid leakage detection unit detects whether there is liquid leakage below the gun head in real time, and controls the buzzer 3 to emit a warning sound if there is liquid leakage; when the gun head is moved to extend into the test tube, the position detection unit controls the liquid leakage detection unit to stop running. Through the mutual matching and use among the position detection unit, the liquid leakage detection unit and the buzzer 3, whether there is liquid leakage in the pipette 2 can be detected in the way that whether there is liquid leakage (droplet) below the gun head when the gun head is moved out of the test tube, and if there is liquid leakage in the pipette 2, the on-site operator can be prompted in time to repair and maintain the pipette 2 through the buzzer 3.

[0037] Referring to Fig. 1 and Fig. 2 The bottom of the fixed table 1 is fixedly installed with a guide rod 11, the guide rod 11 is slidably installed with a connecting plate 12, and the connecting plate 12 is detachably connected with the driving rod 13; the position detection unit comprises an infrared distance measuring sensor 4 which is fixedly installed on the connecting plate 12 and is used for detecting the distance between the connecting plate 12 and the bottom surface of the fixed table 1 and outputting a sensing distance signal, and a first single-chip microcomputer which is connected with the signal output end of the infrared distance measuring sensor 4 and is used for receiving the sensing distance and outputting a detection control signal when the sensing distance is less than a set value.

[0038] In the process that the driving rod 13 drives the gun head of the pipette 2 to move up and down, the connecting plate 12 and the infrared distance measuring sensor 4 can be synchronously driven to move up and down, since the position of the gun head on the driving rod 13 is fixed, the first single-chip microcomputer can calculate whether the gun head extends into or out of the test tube through comparison and calculation, and output a detection control signal when the gun head extends out of the test tube, so as to control the liquid leakage detection unit to detect the liquid leakage of the gun head of the pipette 2.

[0039] Referring to Fig. 1The leakage detection unit comprises an ultrasonic sensor 5 of model Bonner M25U, which is fixedly installed on the inner wall of the cabinet of the multi-channel polypeptide solid-phase synthesis reaction device, located between the gun head and the test tube support, and has the sensing end facing the gun head, for detecting the liquid drops falling through the gun body and outputting a leakage sensing signal; and a second single-chip microcomputer, which is in signal connection with the signal output end of the ultrasonic sensor 5, for receiving the leakage sensing signal and outputting a first warning signal.

[0040] The ultrasonic waves emitted by the ultrasonic transmitter in the ultrasonic sensor 5 are received by the ultrasonic receiver in the ultrasonic sensor 5 after contacting the liquid drops, and whether there is the liquid drops falling below the gun head can be judged by comparing the strengths of the ultrasonic signals. If there is the liquid drops falling, the ultrasonic sensor 5 outputs the leakage sensing signal, and the second single-chip microcomputer can output the first warning signal to control the buzzer 3 to emit the warning sound.

[0041] With reference to Fig. 2 The side wall of the driving rod 13 is fixedly installed with a mounting plate 14, two first through holes 15 are provided through the mounting plate 14, two second through holes 16 matched with the two first through holes 15 are provided through the connecting plate 12, and the mounting plate 14 and the connecting plate 12 are connected through two bolts 17. Through the first through holes 15 in the mounting plate 14 and the second through holes 16 in the connecting plate 12, the connecting plate 12 and the mounting plate 14 can be connected through the two bolts 17 by the operator.

[0042] The implementation principle of the multi-channel polypeptide solid-phase synthesis reaction device is as follows: in the process of driving the gun head of the pipettor 2 to move up and down by the driving rod 13, the connecting plate 12 and the infrared distance measuring sensor 4 can be synchronously driven to move up and down. Since the position of the gun head on the driving rod 13 is fixed, the first single-chip microcomputer can calculate whether the gun head is inserted into or withdrawn from the test tube by comparison and calculation, and output a detection control signal when the gun head is withdrawn from the test tube, so as to control the leakage detection unit to detect the leakage of the gun head of the pipettor 2. The ultrasonic waves emitted by the ultrasonic transmitter in the ultrasonic sensor 5 are received by the ultrasonic receiver in the ultrasonic sensor 5 after contacting the liquid drops, and whether there is the liquid drops falling below the gun head can be judged by comparing the strengths of the ultrasonic signals. If there is the liquid drops falling, the ultrasonic sensor 5 outputs the leakage sensing signal, and the second single-chip microcomputer can output the first warning signal to control the buzzer 3 to emit the warning sound.

[0043] Embodiment 2

[0044] Please refer to Fig. 3In the embodiment, the mounting groove 18 is arranged on the connecting plate 12 and located between the two second through holes 16; the pressure sensor 6 is embedded in the mounting groove 18; the pressure sensor 6 detects the contact pressure between the mounting plate 14 and the connecting plate 12 and outputs a contact pressure signal; the signal output end of the pressure sensor 6 is signal connected with the signal output end of the second single-chip microcomputer; the second single-chip microcomputer receives the contact pressure signal and outputs a second warning signal when the contact pressure is greater than a set pressure value; the buzzer 3 receives the second warning signal and emits a warning sound.

[0045] During the operation of the multi-channel polypeptide solid-phase synthesis reaction device, the pressure sensor 6 detects the contact pressure between the mounting plate 14 and the connecting plate 12 in real time; when the electric push rod is abnormal and causes the gun head to collide with the lower mechanism (a test tube support, etc.), the contact pressure detected by the pressure sensor 6 is greater than the set pressure value; the second single-chip microcomputer controls the buzzer 3 to emit a warning sound.

[0046] Through the mutual matching and use of the pressure sensor 6, the second single-chip microcomputer and the buzzer 3, the buzzer 3 can be controlled to emit an alarm when the gun head collides, the technical effect of automatically detecting the collision of the gun head can be achieved, and the functional diversity of the multi-channel polypeptide solid-phase synthesis reaction device is improved.

[0047] The above are preferred embodiments of the present application, and do not limit the protection scope of the present application, so: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A multi-channel polypeptide solid-phase synthesis reaction apparatus, characterized in that, Includes a fixed platform (1), a pipette (2) which is adjustable and height-mounted on the fixed platform (1), and: The position detection unit is installed on the drive rod (13) of the electric push rod on the fixed platform (1) to detect the height of the pipette tip (2) and output a detection control signal when the pipette tip is removed from the test tube; The leakage detection unit is fixedly installed on the inner wall of the cabinet and is connected to the signal output terminal of the position detection unit. It is used to receive the detection control signal and output a first warning signal when leakage occurs below the tip of the pipette (2). A buzzer (3) is fixedly installed on a fixed platform (1) and connected to the signal output terminal of the leakage detection unit. It is used to receive the first warning signal and emit a warning sound.

2. The multi-channel polypeptide solid-phase synthesis reaction apparatus according to claim 1, characterized in that, A guide rod (11) is fixedly installed at the bottom of the fixed platform (1), and a connecting plate (12) is slidably installed on the guide rod (11). The connecting plate (12) is detachably connected to the drive rod (13); the position detection unit includes: An infrared ranging sensor (4) is fixedly installed on the connecting plate (12) to detect the distance between itself and the bottom surface of the fixed platform (1) and output a sensing distance signal. The first microcontroller is connected to the signal output terminal of the infrared ranging sensor (4) to receive the sensing distance and output the detection control signal when the sensing distance is less than the set value.

3. The multi-channel polypeptide solid-phase synthesis reaction apparatus according to claim 2, characterized in that, The leakage detection unit includes: The ultrasonic sensor (5) is fixedly installed on the inner wall of the cabinet of the multi-channel polypeptide solid phase synthesis reaction device, located between the gun tip and the test tube holder, with the sensing end facing the gun tip, to detect droplets falling through the gun body and output a leakage sensing signal. The second microcontroller is connected to the signal output terminal of the ultrasonic sensor (5) and is used to receive the leakage sensing signal and output the first warning signal.

4. The multi-channel polypeptide solid-phase synthesis reaction apparatus according to claim 3, characterized in that, A mounting plate (14) is fixedly installed on the side wall of the drive rod (13). Two first through holes (15) are opened through the mounting plate (14). Two second through holes (16) that are adapted to the two first through holes (15) are opened through the connecting plate (12). The mounting plate (14) and the connecting plate (12) are connected by two bolts (17).

5. The multi-channel polypeptide solid-phase synthesis reaction apparatus according to claim 4, characterized in that, The connecting plate (12) is provided with a mounting groove (18), which is located between the two second through holes (16); A pressure sensor (6) is embedded in the mounting groove (18). The pressure sensor (6) detects the contact pressure between the mounting plate (14) and the connecting plate (12) and outputs a contact pressure signal. The signal output terminal of the pressure sensor (6) is connected to the signal output terminal of the second microcontroller. The second microcontroller receives the contact pressure signal and outputs a second warning signal when the contact pressure is greater than the set pressure value. The buzzer (3) receives the second warning signal and emits a warning sound.

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