Sample stage and dyeing device

Abstract

The utility model discloses a sample table and a staining device, belongs to cell staining field, the sample table is characterized in that a choke weir, an end plate and two cofferdams extend from the edge of a shelving plate, the two cofferdams are positioned on two sides of the shelving plate, the two cofferdams are parallel to each other and are perpendicular to the shelving plate, the end plate and the choke weir are respectively positioned at two ends of the shelving plate, the choke weir is perpendicular to the shelving plate, and the end plate is arranged on the shelving plate. The end portions of the cofferdams extend out of the end plates, the shelving plate, the flow blocking weir, the end plates and the two cofferdams jointly form a bearing area of an open structure, the drainage groove is located in the intersection position of the shelving plate and the cofferdams, and the drainage groove extends to the flow blocking weir to enable the flow blocking weir to be disconnected from the cofferdams. Through structural size matching of the cofferdam and the choked flow weir, under the action of surface tension of the reagent, the reagent is restrained in the bearing area without overflowing, a cover film is not needed, and due to the open design of the bearing area, the reagent is in full contact with the atmosphere and does not generate bubbles; no other operation is needed for removing bubbles, and the dyeing reaction process is simplified.

Description

Technical Field

[0001] This invention relates to the field of cell staining, and in particular to a sample stage and a staining apparatus including the sample stage. Background Technology

[0002] Existing immunohistochemical staining techniques, through processing and staining diseased tissue samples, outline changes in cell and nucleus morphology, thereby detecting the expression of target proteins or nucleic acids. This is used to diagnose diseases and to detect changes in the morphology, structure, function, and metabolism of diseased organisms during disease progression and disease outcome. Furthermore, it can provide the necessary theoretical basis and practical evidence for the diagnosis, treatment, and prevention of diseases.

[0003] In existing immunohistochemical staining techniques, due to the high cost of the staining reaction liquid, a very narrow reaction cavity is usually formed on the surface of the slide. Then, a very small amount of reaction liquid is injected into the narrow reaction cavity to form a liquid cover film on the surface of the tissue sample, so that the reaction liquid reacts fully with the tissue sample.

[0004] Currently, the main method for forming a narrow reaction chamber is to place a solid cover film on the surface of a glass slide. The desired reaction chamber is formed through the cooperation between the solid cover film and the glass slide. For example, invention patent application CN110987559A discloses a cover film for processing glass slide specimens. A recess in the cover film forms a liquid reservoir with the glass slide, serving as the reaction chamber for the reaction liquid and tissue sample. Invention patent application CN111562164A, based on CN110987559A, modifies the structure to form a negative pressure groove with constant negative pressure. This allows the reaction liquid to quickly fill or drain the reaction chamber, prioritizing the processing efficiency of biological samples.

[0005] The cover plate structure of the two patents creates a reaction chamber with a fixed volume. Furthermore, when bubbles are generated during the injection of the reaction liquid, it is difficult to remove them, resulting in uneven reaction and affecting the determination of the results. Utility Model Content

[0006] To overcome the shortcomings of the prior art, one of the objectives of this utility model is to provide an open sample stage that does not require a cover film and does not generate air bubbles.

[0007] In order to overcome the shortcomings of the prior art, the second objective of this utility model is to provide a dyeing device that does not require a covering film and does not generate bubbles.

[0008] One of the objectives of this utility model is achieved through the following technical solution:

[0009] The sample stage includes a support plate, a flow-blocking weir, an end plate, and two surrounding weirs. The flow-blocking weir, the end plate, and the two surrounding weirs extend from the edge of the support plate. The two surrounding weirs are located on both sides of the support plate, are parallel to each other, and are perpendicular to the support plate. The end plate and the flow-blocking weir are located at both ends of the support plate. The flow-blocking weir is perpendicular to the support plate, and the end of the surrounding weir extends from the end plate. The support plate, the flow-blocking weir, the end plate, and the two surrounding weirs together form a load-bearing area. The upper part of the load-bearing area has an open structure. The sample stage is also provided with a flow-draining channel. The flow-draining channel is located at the intersection of the support plate and the surrounding weirs. The flow-draining channel is arranged along the length of the sample stage and extends to the flow-blocking weir, thus disconnecting the flow-blocking weir from the surrounding weirs.

[0010] Furthermore, the diversion channel includes a horizontal section and an inclined section connected to the horizontal section. The horizontal section is located at the end of the diversion channel away from the obstruction weir, and the inclined section is located at the end of the diversion channel close to the obstruction weir.

[0011] Furthermore, there are two diversion channels, which are symmetrically arranged and located inside the two cofferdams.

[0012] Furthermore, the height of the end plate is greater than the height of the cofferdam.

[0013] Furthermore, the height of the flow-blocking weir is greater than the height of the cofferdam.

[0014] The second objective of this utility model is achieved by the following technical solution:

[0015] A staining apparatus includes a housing, a drive unit, a staining assembly, a waste liquid channel, and a nozzle. The drive unit is mounted on the housing, the staining assembly is rotatably mounted on the housing, the waste liquid channel is fixed to the housing and located at the end of the staining assembly, and the nozzle faces the staining assembly. The staining assembly includes any of the above-mentioned sample stages. The drive unit drives the staining assembly to rotate relative to the housing, and the waste liquid channel receives the waste liquid flowing out of the drainage tank.

[0016] Furthermore, the staining assembly also includes a base and a heating film, the sample stage is fixedly connected to the base, and the heating film is located between the base and the sample stage.

[0017] Furthermore, the staining assembly also includes a mounting column, which connects the sample stage to the base and is made of an elastic material.

[0018] Furthermore, the staining assembly also includes an oscillator, which is installed between the sample stage and the base, and the oscillator drives the sample stage to vibrate.

[0019] Furthermore, the driving component is an electric cylinder.

[0020] Compared to existing technologies, the sample stage of this utility model includes a support plate, a flow-blocking weir, an end plate, and two surrounding weirs. The flow-blocking weir, end plate, and two surrounding weirs extend from the edge of the support plate. The two surrounding weirs are located on both sides of the support plate, are parallel to each other, and are perpendicular to the support plate. The end plate and the flow-blocking weir are located at both ends of the support plate, with the flow-blocking weir perpendicular to the support plate. The ends of the surrounding weirs extend from the end plates. The support plate, flow-blocking weir, end plate, and two surrounding weirs together form a load-bearing area. The upper part of the load-bearing area has an open structure. The sample stage is also provided with a flow-draining groove for flow drainage. The trough is located at the intersection of the support plate and the confining dam. The drainage trough is set along the length of the sample stage and extends to the choke weir, which disconnects the choke weir from the confining dam. With the above design, the staining reagent is dropped onto the sample slide. Through the structural dimensions of the confining dam and the choke weir, the reagent can be constrained in the bearing area under the action of the surface tension of the reagent and will not overflow. This eliminates the need for a cover film on the sample stage. The open design of the bearing area allows the reagent to fully contact the atmosphere without generating bubbles. Therefore, no other operation is needed to remove the bubbles, which greatly simplifies the staining reaction process. Attached Figure Description

[0021] Figure 1 This is a perspective view of the dyeing apparatus of this utility model;

[0022] Figure 2 for Figure 1 An exploded view of the staining apparatus;

[0023] Figure 3 for Figure 1 A three-dimensional view of the dyeing components of the dyeing apparatus;

[0024] Figure 4 for Figure 3 An exploded view of the staining components;

[0025] Figure 5 for Figure 4 A three-dimensional view of the sample stage of the staining component;

[0026] Figure 6 for Figure 4 A three-dimensional sectional view of the dyeing components;

[0027] Figure 7 for Figure 1 A three-dimensional sectional view of the dyeing apparatus.

[0028] In the figure: 10, shell; 11, base plate; 12, side plate; 20, drive component; 30, staining assembly; 31, base; 310, mounting hole; 32, mounting column; 33, vibrator; 34, heating film; 35, sample stage; 350, shelf; 351, dike; 352, flow-blocking weir; 353, drainage channel; 3530, horizontal section; 3531, inclined section; 354, end plate; 40, rotating shaft; 50, waste liquid channel; 60, nozzle; 200, sample slide. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or it can be fixed through another intermediate component. When a component is said to be "connected to" another component, it can be directly connected to the other component or it may be fixed through another intermediate component. When a component is said to be "set on" another component, it can be set directly on the other component or it may be set through another intermediate component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0032] Please see Figure 1 as well as Figure 2 The staining device of this application is used to process and stain diseased tissue samples, outline changes in cell and nucleus morphology, and detect the expression of target proteins or nucleic acids, so as to diagnose diseases and the morphological, structural and functional metabolic changes of diseased organisms and disease outcomes during the disease process.

[0033] The dyeing device includes a housing 10, a drive unit 20, a dyeing assembly 30, a rotating shaft 40, a waste liquid channel 50, and a nozzle 60.

[0034] The housing 10 includes a base plate 11 and a side plate 12 fixed to the base plate 11, the base plate 11 and the side plate 12 forming a hollow structure.

[0035] The driving component 20 is installed inside the housing 10. One end of the driving component 20 is fixed to the base plate 11, and the other end of the driving component 20 is connected to the dyeing assembly 30. The driving component 20 and the dyeing assembly 30 are flexibly connected so that the dyeing assembly 30 is in close contact with the housing 10 when it rotates to a horizontal position. In this embodiment, the driving component 20 is an electric cylinder or a pneumatic cylinder.

[0036] Please see Figures 3 to 6 The staining assembly 30 includes a base 31, a mounting post 32, a vibrator 33, a heating film 34, and a sample stage 35. The base 31 and the sample stage 35 are connected by the mounting post 32, which is made of an elastic material. This allows the base 31 and sample stage 35 to be elastically connected, effectively transmitting the vibration of the vibrator 33 to the sample stage 35 and accelerating the staining reaction. Specifically, the mounting post 32 is made of any one of a spring, rubber, or polyurethane.

[0037] The oscillator 33 and the heating film 34 are installed between the base 31 and the sample stage 35. The oscillator 33, fixed to the heating film 34, provides vibration to promote the mixing of staining reagents and facilitate efficient reaction. The heating film 34 is fixed to the back of the sample stage 35 to meet the temperature requirements of the staining reaction.

[0038] The sample stage 35 includes a support plate 350, two cofferdams 351, a flow-blocking weir 352, and an end plate 354. The two cofferdams 351, the flow-blocking weir 352, and the end plate 354 all extend from the support plate 350, and together they form a load-bearing area. The two cofferdams 351, the flow-blocking weir 352, and the end plate 354 are all perpendicular to the support plate 350. The two cofferdams 351 are located on opposite sides of the support plate 350 and are parallel to each other. The flow-blocking weir 352 and the end plate 354 are located at opposite ends of the support plate 350. The height of the end plate 354 is greater than the height of the cofferdams 351, and the height of the flow-blocking weir 352 is greater than the height of the cofferdams 351. The height of the two cofferdams 351, the flow-blocking weir 352, and the end plate 354 is greater than the height of the sample slide 200.

[0039] The sample stage 35 is also provided with a flow channel 353, which is arranged along the length of the sample stage 35 and extends from the bearing area to the outside. Specifically, the flow channel 353 is located at the intersection of the cofferdam 351 and the support plate 350, and includes a horizontal section 3530 and an inclined section 3531 connected to the horizontal section 3530. The horizontal section 3530 is located at the end of the flow channel 353 away from the flow-blocking weir 352, and the inclined section 3531 is located at the end of the flow channel 353 closer to the flow-blocking weir 352. The inclined section 3531 of the flow channel 353 disconnects the flow-blocking weir 352 from the cofferdam 351. In this embodiment, there are two flow channels 353, which are symmetrically arranged.

[0040] Please continue reading. Figure 7 When using the staining apparatus, place the sample slide 200 containing the sample in the support area of ​​the sample stage 35; move the nozzle 60 to the front end of the support area, while simultaneously tilting the staining assembly 30 so that its tail is in contact with the waste liquid channel 50; the nozzle 60 sprays a high-speed cleaning liquid stream to clean the sample; after cleaning, the staining assembly 30 returns to the horizontal position, and the nozzle 60 moves out of the support area; add a certain reagent to the sample slide 200, start the oscillator, start the heating, and move the nozzle 60 to the support area to spray a low-speed airflow to disturb the reagent and enhance mixing; according to the needs of the staining reaction, the incubation lasts for a certain period of time; after incubation, repeat steps 2-5.

[0041] During use, after the sample slide 200 is placed in the support area, the structural height of the weir 351 and the flow-blocking weir 352 of the sample stage 35 is slightly higher than the upper surface of the sample slide 200. When the staining reagent is added to the sample slide 200, the structural dimensions of the weir 351 and the flow-blocking weir 352, combined with the surface tension of the reagent, can constrain the reagent in the slide area without overflowing. During incubation, the sample needs to be heated. The upper part of the support area has an open structure, and because it is in full contact with the atmosphere, the reagent will not generate bubbles. Therefore, no other operation is needed to remove the bubbles, greatly simplifying the staining reaction process. During incubation, the oscillator 33 starts to vibrate and provides a mixing effect to the reagent on the sample stage 35 through an elastic connection, and the nozzle 6... The device moves to the carrying area and sprays a low-speed airflow to agitate the reagent and enhance mixing. At this time, under the action of the reagent surface tension, the weir 351 and the choke weir 352 can work together to restrain the reagent in the carrying area without overflowing. When the incubation ends, the drive unit 20 controls the staining assembly 30 to flip and tilt. In this state, the staining reagent will no longer be constrained by the choke weir 352 under the action of gravity, but the staining reagent cannot be completely discharged by itself. During the sample cleaning process, the nozzle 60 sprays a high-speed cleaning liquid to ensure that the sample is clean. The notch of the choke weir 352 and the drainage groove 353 can guide the waste liquid to be discharged quickly without residue, so as not to affect the staining reaction effect in the next step. After rinsing, the staining assembly 30 returns to the horizontal position and is ready for the next stage of staining reaction.

[0042] The above embodiments only illustrate several implementation methods of this utility model, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the utility model patent. It should be noted that for those skilled in the art, several modifications and improvements can be made without departing from the concept of this utility model. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of this utility model, and all of these fall within the protection scope of this utility model.

Claims

1. A sample stage, including a shelf, characterized in that: The sample stage also includes a flow-blocking weir, an end plate, and two surrounding weirs. The flow-blocking weir, the end plate, and the two surrounding weirs extend from the edge of the support plate. The two surrounding weirs are located on both sides of the support plate, are parallel to each other, and are perpendicular to the support plate. The end plate and the flow-blocking weir are located at both ends of the support plate. The flow-blocking weir is perpendicular to the support plate, and the end of the surrounding weir extends from the end plate. The support plate, the flow-blocking weir, the end plate, and the two surrounding weirs together form a bearing area. The upper part of the bearing area is an open structure. The sample stage is also provided with a flow-draining channel. The flow-draining channel is located at the intersection of the support plate and the surrounding weirs. The flow-draining channel is arranged along the length of the sample stage and extends to the flow-blocking weir, thus disconnecting the flow-blocking weir from the surrounding weirs.

2. The sample stage according to claim 1, characterized in that: The diversion channel includes a horizontal section and an inclined section connected to the horizontal section. The horizontal section is located at the end of the diversion channel away from the obstruction weir, and the inclined section is located at the end of the diversion channel close to the obstruction weir.

3. The sample stage according to claim 1, characterized in that: There are two diversion channels, which are symmetrically arranged and located inside the two cofferdams.

4. The sample stage according to claim 1, characterized in that: The height of the end plate is greater than the height of the cofferdam.

5. The sample stage according to claim 1, characterized in that: The height of the flow-blocking weir is greater than the height of the cofferdam.

6. A dyeing apparatus, comprising a housing, characterized in that: The staining apparatus further includes a drive unit, a staining assembly, a waste liquid channel, and a nozzle. The drive unit is installed on the housing, the staining assembly is rotatably installed on the housing, the waste liquid channel is fixed to the housing and located at the end of the staining assembly, and the nozzle is directly opposite the staining assembly. The staining assembly includes a sample stage as described in any one of claims 1-5. The drive unit drives the staining assembly to rotate relative to the housing, and the waste liquid channel receives the waste liquid flowing out of the drainage trough.

7. The staining apparatus according to claim 6, characterized in that: The staining assembly also includes a base and a heating film, the sample stage is fixedly connected to the base, and the heating film is located between the base and the sample stage.

8. The staining apparatus according to claim 7, characterized in that: The staining assembly also includes a mounting column, which connects the sample stage to the base. The mounting column is made of an elastic material.

9. The staining apparatus according to claim 7, characterized in that: The staining assembly also includes a vibrator, which is installed between the sample stage and the base, and the vibrator drives the sample stage to vibrate.

10. The staining apparatus according to claim 6, characterized in that: The driving component is an electric cylinder.

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