Material sheet sterilization verification card box

Abstract

The utility model provides a material sheet sterilization verification card box, a liquid storage bag, a waste liquid bag, a peristaltic pump, an electromagnetic valve and a reaction chip are arranged in a chip card box, a plurality of sterilization reaction chambers are arranged on the reaction chip, a plurality of parallel experiments can be carried out, and different devices are connected through hoses. The culture solution in the liquid storage bag flows into different reaction chambers under the action of the peristaltic pump and under the control of the reaction chip and the electromagnetic valve, is subjected to microbial sterilization parallel experiments and finally flows into the waste liquid bag. The reaction chip can be used for optical signal acquisition, and real-time observation can be carried out through a microscope. The chip card box provided by the utility model is designed to be totally closed, and is high in integration level, simple to operate, safe and reliable.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biological protection, concretely relates to a material sheet sterilization verification card box. BACKGROUND

[0002] Material protection coating sterilization technology is an effective means to prevent material corrosion. The traditional research method is to observe the material sheet soaked in the microbial culture solution, which cannot achieve dynamic culture, cannot truly reflect the killing effect of different materials on microorganisms, and has large culture volume, large culture quantity and time-consuming and laborious. Therefore, an integrated high-throughput sterilization system is urgently needed to truly simulate the killing process of microorganisms on the material sheet and quickly complete the sterilization verification of different materials on microorganisms. UTILITY MODEL CONTENT

[0003] In order to overcome the problems in the prior art, the utility model provides an integrated high-throughput material sheet sterilization verification card box.

[0004] The utility model provides the following technical scheme:

[0005] A material sheet sterilization verification card box, comprising a liquid storage bag and a reaction chip sealed in a box body, a microbial culture chamber and a sterilization reaction chamber connected by a liquid path are arranged in the reaction chip, microbial freeze-dried powder is stored in the microbial culture chamber, and a sterilization material sheet is fixed in the sterilization reaction chamber.

[0006] Further, the position corresponding to the sterilization reaction chamber on the box body is made of light-transmitting material.

[0007] Further, a dyeing chamber is connected to the downstream of the microbial culture chamber through a liquid mixing channel, a PBS buffer inlet and a dye inlet are arranged on the upstream of the liquid mixing channel, the liquid mixing channel is alternately connected by a main pipeline-branch pipeline-main pipeline, a second liquid storage bag stores PBS buffer, and the PBS buffer inlet is connected to the second liquid storage bag by a peristaltic pump liquid path.

[0008] Further, the sterilization reaction chamber comprises a plurality of chambers, the dyeing chamber is connected to the sterilization reaction chamber through a liquid distribution channel, an electromagnetic valve is arranged at the liquid inlet of the liquid distribution channel to control the liquid on-off between the dyeing chamber and the liquid distribution channel, a main pipeline of the liquid distribution channel is communicated with a gas pump inlet, the gas pump inlet is connected to a micro gas pump, and a branch pipeline is connected to the liquid inlet path of the sterilization reaction chamber through an electromagnetic valve.

[0009] Further, the bottom of the sterilization reaction chamber is provided with a liquid outlet connected to a waste liquid bag.

[0010] Furthermore, the reaction chip has an irregularly shaped positioning structure on its side and positioning holes on its surface.

[0011] Furthermore, the bactericidal material sheet is a metal sheet with a nanostructure on its surface.

[0012] Furthermore, the fluorescent dye is DMAO, AIE, or EthD-III.

[0013] By adopting the above technical solution, this utility model has the following beneficial effects:

[0014] (1) The chip cartridge of this utility model can complete the study of the microbial killing effect of the material sheet in a closed liquid channel, which is safe and reliable;

[0015] (2) The card box of this utility model has a high degree of integration and can complete the experiment automatically through electronic control without manual operation;

[0016] (3) This utility model has multiple experimental groups, which can carry out multiple parallel experiments at the same time. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the material sheet sterilization verification card box of this utility model;

[0018] Figure 2 This is a schematic diagram of the reaction chip structure of the material sheet sterilization verification card box of this utility model.

[0019] The components are: 1-reaction chip, 2-box body, 3-solenoid valve, 4-waste liquid bag, 5-micro air pump, 6-peristaltic pump, 7-storage bag, 8-microbial culture chamber, 9-liquid mixing channel, 10-staining chamber, 11-sterilization reaction chamber, 12-irregular positioning structure, 13-positioning hole, 14-non-detachable screw, 15-electrical connector, 16-air pump inlet, 17-dispensing channel. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the structural diagrams and specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model.

[0021] Example 1

[0022] like Figure 1 , Figure 2As shown, this utility model provides a chip cartridge for verifying the sterilization effect of a material sheet, including a storage bag 7 and a reaction chip 1 sealed within a casing 2. The reaction chip contains a microbial culture chamber 8 and a sterilization reaction chamber 11 connected by a liquid circuit. The microbial culture chamber stores lyophilized microbial powder, while the sterilization reaction chamber contains a sterilization material sheet. An electromagnetic valve 3 is installed on the liquid inlet path of the sterilization reaction chamber. The first storage bag stores culture medium, which is connected to the microbial culture chamber of the reaction chip via a peristaltic pump 6. The culture medium in the storage bag enters the microbial culture chamber through the peristaltic pump, reviving and culturing the microorganisms to form a bacterial solution. The electromagnetic valve controls the flow of culture medium liquid from the outlet of the storage bag into the sterilization reaction chamber. The lyophilized microbial powder in the microbial culture chamber can be various bacterial lyophilized powders, and the sterilization material sheet in the sterilization reaction chamber is a metal sheet with a nanostructure on its surface. Through the friction and cutting action of the nanostructure, the bacteria are broken down, achieving a sterilization effect.

[0023] The box body has a light-transmitting material at the position corresponding to the sterilization reaction chamber, which allows for the acquisition of optical signals within the sterilization reaction chamber.

[0024] Preferably, the staining chamber 10 is connected downstream of the microbial culture chamber via a liquid mixing channel 9. Upstream of the liquid mixing channel are a PBS buffer inlet and a dye inlet. The liquid mixing channel is alternately connected by a main line, branch lines, and a main line. A second reservoir bag storing PBS buffer is connected to the PBS buffer inlet via a peristaltic pump circuit. A third reservoir bag storing fluorescent dye is connected to the dye inlet via a peristaltic pump circuit. In this way, the PBS buffer, fluorescent dye, and culture medium are mixed and then enter the staining chamber. The fluorescent dye can be DMAO, AIE, EthD-III, etc., which mixes with the bacteria in the staining chamber and enters the bacteria to achieve staining.

[0025] The sterilization reaction chamber consists of several chambers. The staining chamber is connected to the sterilization reaction chamber via a liquid distribution channel 17. A solenoid valve is installed at the inlet of the liquid distribution channel to control the flow of liquid between the staining chamber and the liquid distribution channel. The main pipe of the liquid distribution channel is connected to the air pump inlet 16, which is connected to a miniature air pump 5. The branch pipe is connected to the liquid inlet passage of the sterilization reaction chamber via a solenoid valve. Opening the solenoid valve starts the air pump, which accelerates the spraying of liquid in the liquid distribution channel onto the sterilization material sheet, giving the bacteria a relatively high initial velocity, allowing the nanostructure to contact the bacteria more fully, and improving the sterilization effect of the nanostructure on the bacteria.

[0026] The sterilization reaction chamber has a liquid outlet at the bottom, which is connected to a waste liquid bag 4. Excess liquid in the chamber flows out from the outlet and is stored in the waste liquid bag. An electrical connector 15 is also provided inside the box for supplying power to the various electrical components.

[0027] The reaction chip has a uniquely shaped positioning structure 12 on its side for fixing the reaction chip to the housing. The reaction chip has positioning holes 13 for positioning the reaction chip. Locking screws 14 are located at the top diagonal of the housing, allowing for easy fixing and disassembly of the external structure, preventing the housing from shaking during experiments and simplifying operation.

[0028] The embodiments described above are merely illustrative of the implementation of this utility model, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A material sheet sterilization verification card box, comprising a liquid storage bag and a reaction chip sealed inside the box, characterized in that, The reaction chip has a microbial culture chamber and a sterilization reaction chamber connected by a liquid circuit. The microbial culture chamber stores lyophilized microbial powder, and the sterilization reaction chamber has a sterilization material sheet fixed in it. A solenoid valve is provided on the liquid inlet passage of the sterilization reaction chamber. The first liquid storage bag stores culture medium and is connected to the microbial culture chamber of the reaction chip through a peristaltic pump.

2. The material sheet sterilization verification card box according to claim 1, characterized in that, The position on the box body corresponding to the sterilization reaction chamber is made of light-transmitting material.

3. The material sheet sterilization verification card box according to claim 1, characterized in that, Downstream of the microbial culture chamber, a liquid mixing channel connects to the staining chamber. Upstream of the liquid mixing channel, there are PBS buffer inlets and dye inlets. The liquid mixing channel is alternately connected by a main line, a branch line, and a main line. The second reservoir bag stores PBS buffer and is connected to the PBS buffer inlet via a peristaltic pump. The third reservoir bag stores fluorescent dye and is connected to the dye inlet via a peristaltic pump.

4. The material sheet sterilization verification card box according to claim 3, characterized in that, The sterilization reaction chambers are of several types. The staining chambers are connected to the sterilization reaction chambers through a liquid distribution channel. The liquid distribution channel is equipped with a solenoid valve at the inlet to control the liquid flow between the staining chambers and the liquid distribution channel. The main pipe of the liquid distribution channel is connected to the air pump inlet, which is connected to a miniature air pump. The branch pipes are connected to the liquid inlet passage of the sterilization reaction chambers through solenoid valves.

5. The material sheet sterilization verification card box according to claim 1, characterized in that, The sterilization reaction chamber has a liquid outlet at the bottom, which is connected to a waste liquid bag.

6. The material sheet sterilization verification card box according to claim 1, characterized in that, The reaction chip has an irregularly shaped positioning structure on its side and positioning holes on its surface.

7. The material sheet sterilization verification card box according to claim 1, characterized in that, The bactericidal material sheet is a metal sheet with a nanostructure on its surface.

8. The material sheet sterilization verification card box according to claim 3, characterized in that, The fluorescent dye is DMAO, AIE, or EthD-III.

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