Rapid detection kit for synchronously detecting cell factors IFN-gamma and TNF-alpha

Abstract

The utility model provides a rapid detection kit for synchronously detecting cell factors IFN-gamma and TNF-alpha, comprising: a kit body comprising a card box and a box cover which covers the bottom of the card box; the detection card is arranged in the card box; the liquid suction device is connected with the detection card and extends out of the card box, and the box cover hermetically wraps the sample suction device; the detection card comprises a bottom plate and test strips adhered to the bottom plate, and the test strips comprise a first test strip and a second test strip which are symmetrically arranged on the two sides of the surface of the bottom plate; the first test strip comprises a sample pad, a first latex microsphere pad, a first nitrocellulose membrane and absorbent paper which are sequentially lapped; the second test strip comprises a sample pad, a second latex microsphere pad, a second nitrocellulose membrane and absorbent paper which are sequentially lapped. The kit is simple in structure, the kit comprises the two detection cards in parallel, IFN-gamma and TNF-alpha can be synchronously detected at a time, manpower and material resources are greatly saved, and operation is easy and fast.

Description

TECHNICAL FIELD

[0001] The utility model relates to the detection method of cytokine IFN-gamma and TNF-alpha, especially in synchronous detection cytokine IFN-gamma and TNF-alpha's rapid detection kit. BACKGROUND

[0002] Cytokine IFN-gamma and TNF-alpha play an important role in immune response, inflammatory reaction and the pathological process of various diseases, especially in the field of cell therapy drug development, and are widely used in the efficacy evaluation. But the traditional detection method is mostly concentrated in flow cytometry, ELISA and other methods, these methods are often cumbersome, complex operation, time-consuming and high cost, and cannot meet the demand of rapid detection. Therefore, it is particularly important to develop a kind of rapid and accurate cytokine IFN-gamma and TNF-alpha detection device. UTILITY MODEL CONTENT

[0003] The technical problem to be solved by the present application is that there is a lack of a method and device for rapidly and effectively detecting cytokine IFN-gamma and TNF-alpha in the prior art.

[0004] To solve the above technical problems, the present application provides a rapid detection kit for synchronous detection of cytokine IFN-gamma and TNF-alpha, comprising: a box body comprising a card box and a box cover, the box cover being covered with the bottom of the card box; a detection card built-in the card box; a liquid suction device connected to the detection card and extending out of the card box, the box cover sealingly wrapping the sample suction device; the detection card comprises a bottom plate and a test strip pasted on the bottom plate, the test strip comprises first and second test strips symmetrically arranged on both sides of the surface of the bottom plate; the first test strip comprises a sample pad, a first latex microsphere pad, a first nitrocellulose membrane and an absorbent paper connected in sequence; the second test strip comprises a sample pad, a second latex microsphere pad, a second nitrocellulose membrane and an absorbent paper connected in sequence; the two sides of the bottom plate in contact with the inner wall of the card box are provided with outwardly protruding clamping pegs, the inner wall of the card box is provided with clamping grooves matched with the clamping pegs, and the bottom plate is inserted into the clamping grooves through the clamping pegs, so that the bottom plate is embedded in the card box; the first latex microsphere pad is coated with latex microspheres of the first anti-IFN-gamma antibody, and the second latex microsphere pad is coated with latex microspheres of the first anti-TNF-alpha antibody; the first nitrocellulose membrane is provided with a first detection zone and a quality control zone, and the first detection zone is solid-phase with purified second anti-IFN-gamma antibody; the second nitrocellulose membrane is provided with a second detection zone and a quality control zone, and the second detection zone is solid-phase with purified second anti-TNF-alpha antibody; the quality control zone is coated with rabbit anti-mouse IgG polyclonal antibody.

[0005] According to the embodiment of the present application, the card box and the box cover are connected by threads, the bottom of the card box is provided with external threads, and the inner wall of the box cover is provided with internal threads.

[0006] According to the embodiment of the present application, the liquid suction device is a hydrophilic sponge head, and the hydrophilic sponge head is in a bullet head shape; one end of the hydrophilic sponge head is lapped with the sample pad.

[0007] According to the embodiment of the present application, the bottom plate is a PVC bottom plate.

[0008] According to the embodiment of the present application, the box body is made of a plastic transparent material.

[0009] According to the embodiment of the present application, the clamping clip is in a strip shape.

[0010] According to the embodiment of the present application, the box body is in a whole cylindrical shape.

[0011] According to the embodiment of the present application, the first detection strip is close to the first latex microsphere pad, and the second detection strip is close to the second latex microsphere pad.

[0012] According to the embodiment of the present application, the two ends of the first nitrocellulose membrane are respectively lapped below the first latex microsphere pad and the water absorption paper, and the two ends of the second nitrocellulose membrane are respectively lapped below the second latex microsphere pad and the water absorption paper.

[0013] Compared with the prior art, the technical scheme of the present application has the following beneficial effects:

[0014] 1. The device box of the utility model discloses two kinds of detection cards can be arranged side by side, can carry out synchronous detection to cytokine IFN-γ and TNF-α at one time, greatly saves manpower and material resources, and simple operation is swift and efficient, and the detection efficiency is improved obviously.

[0015] 2. The detection card of the utility model is composed of a bottom plate and test strips pasted on the surface of the bottom plate, the bottom plate is provided with clamping clips on both sides, the clamping clips are clamped and matched with the clamping grooves in the card box during installation, the bottom plate is bent to the inside during disassembly, so that the clamping clips are separated from the clamping grooves, the device is convenient to assemble and disassemble, convenient to replace, the card box can be recycled, and cost is saved.

[0016] 3. The card box in the utility model is made of transparent plastic, the whole detection process and detection result can be directly observed through the card box, without additionally setting an observation port, the manufacturing process and time of the card box are reduced, and the overall strength is better. DRAWINGS

[0017] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings of the embodiments will be briefly introduced below, and obviously, the drawings described below only relate to some embodiments of the present application, but not limit the present application.

[0018] Figure 1 It is a structure schematic view of a rapid detection kit for synchronously detecting cytokine IFN-γ and TNF-α.

[0019] Figure 2 The left view of the detection card in an embodiment of the utility model;

[0020] Figure 3 The structural schematic view of the detection card of the utility model;

[0021] Figure 4 The bottom view of the card box in an embodiment of the utility model.

[0022] The signs of the drawings are explained as follows:

[0023] 11. card box, 12. box cover, 21. first test strip, 22. second test strip, 23. bottom plate, 31. hydrophilic sponge head, 111. card slot, 112. clamping card, 211. sample pad, 212. first latex microsphere pad, 213. first nitrocellulose membrane, 214. water absorption paper, 221. second latex microsphere pad, 222. second nitrocellulose membrane, 2131. first detection strip, 2132. quality control strip, 2221. second detection strip.

[0024] Wherein, Figure 2 The arrow indicates the chromatography direction. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme of the embodiments of the present application will be described clearly and completely below in conjunction with the drawings of the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by those of ordinary skill in the art without any creative effort fall within the scope of protection of the present application.

[0026] Unless otherwise defined, the technical terms or scientific terms used herein should be understood as the usual meaning understood by those with ordinary skills in the art to which the present application belongs. The "first", "second" and similar words used in the patent application specification and claims of the present application do not represent any order, quantity or importance, but are only used to distinguish different components. Similarly, "one" or "a" and similar words also do not represent a quantity limit, but represent the existence of at least one.

[0027] As Figure 1 , Figure 2As shown, the utility model discloses a kind of rapid detection kit of synchronous detection cytokine IFN-γ and TNF-α, comprising: box body, including card box 11 and box cover 12, box cover 12 and card box 11 bottom are covered, detection card is built-in in card box 11, liquid suction device is connected detection card and extends out card box 11, box cover 12 seals and wraps suction device. Among them, two test strips are arranged on detection card, respectively for testing cytokine IFN-γ, cytokine TNF-α, relative to prior art, the application can detect two kinds of cytokines simultaneously, save manpower and material resources, significantly improve detection efficiency.

[0028] Specifically, thread connection is used between card box 11 and box cover 12, the bottom of card box 11 is provided with external thread, and the inner wall of box cover 12 is provided with internal thread.

[0029] Specifically, the liquid suction device is a hydrophilic sponge head 31, which can quickly absorb liquid. The hydrophilic sponge head 31 is in the shape of a bullet. Figure 1 As shown, the hydrophilic sponge head 31 is sealed in the box cover 12, thereby protecting the hydrophilic sponge head 31.

[0030] Specifically, the box body can be cylindrical as a whole. The box body is preferably made of transparent plastic material. The entire detection process and detection results can be directly observed through the card box 11 without the need for additional observation ports, reducing the manufacturing process and time of the card box 11, and improving the overall strength.

[0031] In this embodiment, the detection card includes a base plate 23 and test strips attached to the base plate 23. The test strips include first test strips 21 and second test strips 22 symmetrically arranged on both sides of the surface of the base plate 23. Figure 2 As shown, the first test strips 21 include a sample pad 211, a first latex microsphere pad 212, a first nitrocellulose membrane 213 and an absorbent paper 214 connected in sequence. The second test strips 22 include a sample pad 211, a second latex microsphere pad 221, a second nitrocellulose membrane 222 and an absorbent paper 214 connected in sequence.

[0032] Specifically, the base plate 23 can be a PVC base plate 23.

[0033] Specifically, the two ends of the first nitrocellulose membrane 213 are respectively connected below the first latex microsphere pad 212 and the absorbent paper 214, and the two ends of the second nitrocellulose membrane 222 are respectively connected below the second latex microsphere pad 221 and the absorbent paper 214. This design can make the fixation of the nitrocellulose membrane more conducive to detection.

[0034] Specifically, the first latex microsphere pad 212 is coated with latex microspheres of the first anti-IFN-γ antibody, and the second latex microsphere pad 221 is coated with latex microspheres of the first anti-TNF-α antibody.

[0035] In the embodiment, the first nitrocellulose membrane 213 is provided with a first detection zone 2131 and a quality control zone 2132, the first detection zone 2131 is solid-phase coated with purified second anti-IFN-γ antibody; the second nitrocellulose membrane 222 is provided with a second detection zone 2221 and a quality control zone 2132, the second detection zone 2221 is solid-phase coated with purified second anti-TNF-α antibody; the quality control zone 2132 is coated with rabbit anti-mouse IgG polyclonal antibody.

[0036] Specifically, the first detection zone 2131 is close to the first latex microsphere pad 212, and the second detection zone 2221 is close to the second latex microsphere pad 221. The quality control zone on the nitrocellulose membrane is close to one side of the water absorption paper 214.

[0037] It can be understood that the first latex microsphere pad 212 of the first test strip 21 is coated with latex microspheres of the first anti-IFN-γ antibody, and the first detection zone 2131 is solid-phase coated with purified second anti-IFN-γ antibody, so that the first test strip 21 is used to detect whether the IFN-γ is contained in the sample to be treated; the second latex microsphere pad 221 of the second test strip 22 is coated with latex microspheres of the first anti-TNF-α antibody, and the second detection zone 2221 is solid-phase coated with purified second anti-TNF-α antibody, so that the second test strip 22 is used to detect whether the TNF-α is contained in the sample to be treated.

[0038] In the embodiment, the two sides of the bottom plate 23 in contact with the inner wall of the cartridge 11 are provided with outwardly protruding clamping clips 112, the inner wall of the cartridge 11 is provided with clamping grooves 111 matched with the clamping clips 112, and the bottom plate 23 is inserted into the clamping grooves 111 through the clamping clips 112, so that the bottom plate 23 is embedded in the cartridge 11.

[0039] Specifically, the clamping clip 112 can be in a strip shape, and the clamping groove 111 of the inner wall of the cartridge 11 can be a groove recessed in the inner wall of the cartridge 11, or can be a clamping groove protruding from the inner wall of the cartridge 11. The bottom plate 23 is embedded in the cartridge 11, so that the detection card is protected by the cartridge body.

[0040] The use process and principle of the rapid detection kit for synchronously detecting cytokines IFN-gamma and TNF-alpha in the embodiment are as follows: the box cover 12 outside the sponge head is opened, and the special hydrophilic sponge head 31 is directly used for sampling, then observation is waited, and if the sample to be detected contains IFN-gamma or TNF-alpha, the first anti-IFN-gamma antibody or the first anti-TNF-alpha antibody coated on the latex microsphere pad of the detection kit specifically binds to form a complex, then the first anti-IFN-gamma antibody complex specifically binds with the second anti-IFN-gamma antibody, red color appears at the first detection band 2131, which indicates that the sample to be detected is positive and contains IFN-gamma; the first anti-TNF-alpha antibody complex specifically binds with the second anti-TNF-alpha antibody, red color appears at the second detection band 2221, which indicates that the sample to be detected is positive and contains TNF-alpha; if the sample to be detected does not contain IFN-gamma or TNF-alpha, no red color appears on the detection band, and the sample is negative; and the anti-IFN-gamma or TNF-alpha antibody coated on the latex microsphere continues to move forward and combines with the rabbit anti-mouse IgG polyclonal antibody, red color appears on the quality control band, and no matter whether the sample contains IFN-gamma or TNF-alpha, the above-mentioned latex microsphere labeled anti-IFN-gamma or TNF-alpha antibody 1 will continue to move forward to the quality control line and combine with the rabbit anti-mouse IgG polyclonal antibody coated on the quality control line to form a red band, which is the quality control line. If no red color appears in the quality control line during the detection process, it is proved that the latex microspheres are invalid or the operation is wrong, the result is invalid, and the detection needs to be re-detected.

[0041] In summary, the technical scheme of the application has the following beneficial effects:

[0042] 1. The device box of the utility model discloses parallelly equipped with two kinds of detection cards, can one time carry out synchronous detection to cytokine IFN-gamma and TNF-alpha, greatly save manpower and material resources, simple and quick operation, significantly improve the detection efficiency.

[0043] 2. The detection card used in the utility model is composed of a bottom plate and test strips pasted on the surface of the bottom plate, and clamping clips are arranged on the two sides of the bottom plate, so that the clamping clips are clamped and matched with clamping grooves in the card box during installation, and the bottom plate is bent inward during disassembly, so that the clamping clips are separated from the clamping grooves, the device is convenient to assemble and disassemble, convenient to replace, the card box can be recycled, and cost is saved.

[0044] 3. The card box in the utility model is made of transparent plastic, the whole detection process and detection result can be directly observed through the card box, an observation port does not need to be additionally arranged, the manufacturing process and time of the card box are reduced, and the overall strength is better.

[0045] The above merely provides exemplary embodiments of the present application, but not for limiting the protection scope of the present application, which is defined by the appended claims.

Claims

1. A rapid detection kit for simultaneously detecting cytokines IFN-γ and TNF-α, characterized in that, include: The box body includes a card holder and a lid, wherein the lid covers the bottom of the card holder; The detection card is built into the card holder; A liquid aspiration device is connected to the detection card and extends out of the card holder; the box lid seals and encloses the liquid aspiration device. The test card includes a base plate and test strips adhered to the base plate. The test strips include a first test strip and a second test strip symmetrically arranged on both sides of the base plate surface. The first test strip includes a sample pad, a first latex microsphere pad, a first nitrocellulose membrane, and absorbent paper that are sequentially overlapped. The second test strip includes a sample pad, a second latex microsphere pad, a second nitrocellulose membrane, and absorbent paper that are sequentially overlapped. The base plate has outwardly protruding snap-fit ​​clips on both sides that contact the inner wall of the card box, and the inner wall of the card box has card slots that are adapted to the snap-fit ​​clips. The base plate is inserted into the card slots through the snap-fit ​​clips to embed the base plate into the card box. Wherein, the first latex microsphere pad is coated with latex microspheres containing a first anti-IFN-γ antibody, and the second latex microsphere pad is coated with latex microspheres containing a first anti-TNF-α antibody; The first nitrocellulose membrane has a first detection band and a control band, and the first detection band has a purified second anti-IFN-γ antibody on a solid phase; the second nitrocellulose membrane has a second detection band and a control band, and the second detection band has a purified second anti-TNF-α antibody on a solid phase; the control band is coated with rabbit anti-mouse IgG polyclonal antibody.

2. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The card holder and the lid are connected by threads. The bottom of the card holder has external threads, and the inner wall of the lid has internal threads.

3. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The liquid aspiration device is a hydrophilic sponge head, which is bullet-shaped; one end of the hydrophilic sponge head overlaps with the sample pad.

4. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The base plate is a PVC base plate.

5. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The box is made of transparent plastic.

6. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The snap-fit ​​clip is long and narrow.

7. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The box is cylindrical in shape.

8. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The first detection band is close to the first latex microsphere pad, and the second detection band is close to the second latex microsphere pad.

9. The rapid detection kit for simultaneous detection of cytokines IFN-γ and TNF-α according to claim 1, characterized in that, The two ends of the first nitrocellulose membrane overlap the bottom of the first latex microsphere pad and the absorbent paper, respectively, and the two ends of the second nitrocellulose membrane overlap the bottom of the second latex microsphere pad and the absorbent paper, respectively.

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