Device for injecting liquid into embryo egg

Abstract

The utility model provides a device for injecting liquid into embryo eggs, which comprises a tray feeding unit (1), an egg lifting unit (2), a punching unit (3), a film penetrating unit (4), a liquid injection unit (5) and a tray discharging unit (6) which are sequentially arranged along the egg tray conveying direction, the control system is in signal connection with the tray feeding unit (1), the egg lifting unit (2), the punching unit (3), the film penetrating unit (4), the liquid injection unit (5) and the tray discharging unit (6) so as to control the tray feeding unit (1), the egg lifting unit (2), the punching unit (3), the film penetrating unit (4), the liquid injection unit (5) and the tray discharging unit (6) to The egg jacking assembly is arranged, so that embryo eggs with different heights can be equally limited in the guide hole plate; the embryo eggs with different sizes and heights can be directly put into the egg tray, and the embryo eggs do not need to be graded in height in advance; and the pressing force and the egg lifting height of the embryo eggs do not need to be adjusted again in advance due to different heights of the embryo eggs.

Description

Technical Field

[0001] This utility model relates to the technical field of embryo inoculation, specifically to a device for injecting liquid into embryos. Background Technology

[0002] In existing technologies, it is necessary to inject an inoculation liquid into the embryo to observe its impact on the hatching process. For example, in vaccine production, the corresponding virus needs to be injected into the egg for culture while ensuring the survival of the chicken embryo. Therefore, a hole needs to be made in the relatively hard eggshell using a punching needle, and then the virus strain is injected at a suitable location (usually 1 cm below the air cell membrane) using a perforating needle. It is also necessary to ensure that the virus strain is not directly inoculated onto the chicken embryo, otherwise it will cause the chicken embryo to die.

[0003] Existing devices for injecting liquid into embryos still have the following defects and shortcomings in practical use:

[0004] 1) The varying sizes of the embryos result in different heights when placed on the egg tray. Since the punching and perforating needles for the top of the embryos are at the same height, this arrangement ensures that when the height of the top punching and perforating needles matches that of taller eggs, the taller eggs can be successfully punched and perforated. However, for shorter eggs, the punching and perforating needles cannot reach below the air cell membrane, preventing the successful injection of the inoculation liquid. Figure 1 As shown; however, when the height of the top punching needle and the membrane-piercing needle matches that of a lower-height egg, the higher-height egg will cause the punching and membrane-piercing depth to be too deep, resulting in the death of the chicken embryo, as shown. Figure 2 As shown; correspondingly, in order to ensure the smooth drilling and piercing, it is often necessary to grade the embryos in the same egg tray according to their height, and to adjust the stroke of the drilling needle and piercing needle according to the different heights of the embryos fed into the egg tray each time.

[0005] 2) In the existing technology, the embryos need to be punched, perforated and injected manually. The injection site and amount of the inoculation liquid vary from person to person, resulting in different culture effects of the inoculation liquid.

[0006] 3) Manual operation can easily cause product contamination and personnel infection, posing a risk.

[0007] Therefore, there is an urgent need to provide a new solution to address the defects and shortcomings of the existing technologies. Utility Model Content

[0008] In order to overcome the defects and shortcomings of the existing technology, this utility model provides a device for injecting liquid into embryos.

[0009] The technical solution provided by this utility model is as follows:

[0010] A device for injecting liquid into embryo eggs includes an infeeding unit, an egg lifting unit, a punching unit, a membrane-piercing unit, a liquid injection unit, and an outfeeding unit arranged sequentially along the egg tray transport direction. The control system is connected to the infeeding unit, egg lifting unit, punching unit, membrane-piercing unit, liquid injection unit, and outfeeding unit respectively to control them to perform corresponding actions.

[0011] The tray feeding unit horizontally delivers the egg tray containing the embryos to the egg lifting unit position;

[0012] The egg-lifting unit lifts the embryonic eggs in the egg tray from the initial height to the working height of the punching unit;

[0013] The punching unit descends to the top of the embryo and punches holes in the embryo at the current working height.

[0014] After the punching is completed, the membrane-piercing unit descends to the top of the embryo and pierces the embryo at the current working height.

[0015] The injection unit injects the inoculation liquid into a preset location inside the embryo.

[0016] After the liquid injection unit finishes injecting the liquid, the egg lifting unit lowers the embryo to the position of the bottom egg tray;

[0017] The tray delivery unit horizontally delivers the tray containing the injected embryos.

[0018] As a further preferred embodiment of this utility model,

[0019] The egg-lifting unit includes a base plate fixed to the frame, and a lifting cylinder fixed on the top of the base plate. The output end of the lifting cylinder is fixedly connected to the top plate. The top plate can move up and down relative to the base plate along the guide assembly under the drive of the lifting cylinder. The top of the top plate lifts the embryo eggs in the egg tray through the egg-lifting assembly.

[0020] The guide assembly includes a guide rod fixed to the top of the base plate, a sleeve fixedly provided at the bottom of the top plate, the top plate and the sleeve being sleeved on the outer periphery of the guide rod in a liftable manner, and a limiting member extending radially along the guide rod is also fixed at the top of the guide rod.

[0021] The egg-topping assembly includes a pressure device, which is located on the top of the top plate and corresponds to the position of each embryo in the egg tray. An egg-topping elastic element is fixedly provided at the top protruding end of the pressure device. The egg-topping elastic element abuts against the bottom of each embryo in the egg tray. A guide hole plate is also provided on the top of the egg tray, and the internal through holes of the guide hole plate correspond to the position of each embryo in the egg tray.

[0022] As a further preferred embodiment of the present invention, the punching unit includes a punching cylinder fixed on an external bracket. Guide rods are fixed on both sides of the external bracket. A punching bracket is fixedly connected to the extended end of the punching cylinder. The punching bracket is sleeved on the outer periphery of the guide rod and can slide up and down relative to the guide rod. A punching needle mounting part is fixed at the bottom of the punching bracket, and a punching needle is installed inside the punching needle mounting part.

[0023] As a further preferred embodiment of the present invention, the film-piercing unit includes a film-piercing cylinder fixed on an external bracket. The extended end of the film-piercing cylinder is fixedly connected to a film-piercing bracket, and the film-piercing bracket is sleeved on the outer periphery of the guide rod and can slide up and down relative to the guide rod. A film-piercing needle mounting part is fixed at the bottom of the film-piercing bracket, and a film-piercing needle is installed inside the film-piercing needle mounting part.

[0024] As a further preferred embodiment of the present invention, a punching limiting device is provided inside the external bracket, which can limit the lifting stroke of the punching bracket.

[0025] As a further preferred embodiment of the present invention, a membrane-penetrating limiting device is provided inside the punching bracket, which can limit the lifting stroke of the membrane-penetrating bracket.

[0026] As a further preferred embodiment of the present invention, the membrane-penetrating bracket is located inside the perforating bracket and can move up and down relative to the perforating bracket, and the membrane-penetrating needle is located inside the perforating needle and can move up and down relative to the perforating needle.

[0027] As a further preferred embodiment of the present invention, the injection unit includes a storage tank containing inoculation liquid. One end of the storage tank is connected to a collection pipe through a valve. Several joints are evenly distributed on the outer edge of the collection pipe. The joints are connected to a membrane needle at the bottom through pipes and adapters.

[0028] As a further preferred embodiment of the present invention, the pipeline is selectively connected to the adapter and the bottom membrane needle through the opening and closing controller. The opening and closing controller includes a control cylinder fixed to the pipeline frame. The cylinder shaft of the control cylinder can move towards and squeeze the pipeline to extend, or move away from and release the pipeline to retract. An elastic element is also sleeved on the outer periphery of the cylinder shaft. The two ends of the elastic element are fixedly connected to the control cylinder and the cylinder shaft, respectively.

[0029] Furthermore, this utility model also provides a control method for a device for injecting liquid into embryos, characterized by comprising the following steps:

[0030] S1: The tray feeding unit horizontally delivers the egg tray containing the embryos to the egg lifting unit position;

[0031] S2: The egg lifting unit lifts the embryos in the egg tray at the current position to the working height of the punching unit;

[0032] S3: The egg-lifting assembly lifts the embryos in the egg tray into the guide plate;

[0033] S4: The punching unit punches holes in the top of the embryo that is higher than the guide plate;

[0034] S5: After the perforation is completed, the membrane-piercing unit performs the membrane-piercing action on the top of the embryo that is higher than the guide plate;

[0035] S6: Open the valve and the on / off controller, and the inoculation liquid in the storage tank is injected into the preset position inside the embryo through the collection pipe and pipeline;

[0036] S7: After injection, close the valve and the on / off controller;

[0037] S8: The egg-top component descends, dropping the embryo back into the bottom egg tray;

[0038] S9: The egg-lifting unit lowers the embryonic eggs in the egg tray to the initial height;

[0039] S10: The tray delivery unit horizontally delivers the tray containing the embryos.

[0040] The beneficial effects of this utility model compared to the prior art include:

[0041] 1) This utility model provides a device for injecting liquid into embryos. By setting an egg-lifting unit, the egg-top component in the egg-lifting unit can ensure that embryos of different heights are subject to equal limiting in the guide plate. Embryos of different sizes and heights can be directly placed in the egg tray without pre-grading the embryos according to their height. There is no need to readjust the clamping force and lifting height of the embryos in advance because of their different heights.

[0042] 2) This utility model provides a device for injecting liquid into embryos, which can automatically control the injection of liquid into the embryos, and improve the injection efficiency and accuracy, thereby increasing the injection efficiency and reducing the injection cost.

[0043] 3) This utility model provides a device for injecting liquid into embryos. The automatic injection method reduces the potential contamination of the embryos when injecting liquid manually, and also reduces the possibility of contamination and damage to the embryos during manual operation. Attached Figure Description

[0044] Figure 1 This is a schematic diagram of the structure in the existing technology where the drilling and membrane-penetrating working height is only matched with the height of the egg.

[0045] Figure 2This is a schematic diagram of the structure in the existing technology when the working height of the punching and membrane-penetrating process only matches the height of the egg.

[0046] Figure 3 A side view of the structure of the device provided by this utility model.

[0047] Figure 4 A top view of the structure of the device provided by this utility model.

[0048] Figure 5 This is a side view of the structure of the egg-lifting unit before lifting the egg in this utility model.

[0049] Figure 6 This is a side view of the structure of the egg-lifting unit after lifting the egg according to this utility model.

[0050] Figure 7 This is a schematic diagram of the structure of the present invention when the working height of the egg-lifting unit for drilling and membrane insertion is matched with that of all embryos.

[0051] Figure 8 This is a side view of the structure of the punching unit and the membrane-penetrating unit of this utility model.

[0052] Figure 9 This is a side view of the structure of the liquid injection unit of this utility model.

[0053] Figure 10 This is a logic structure diagram of the liquid injection unit of this utility model. Detailed Implementation

[0054] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0055] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0056] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0057] [First Embodiment]

[0058] like Figure 3-10 The image shows a device for injecting liquid into an embryo according to the first embodiment of this utility model. Figure 3-4 As shown, the system includes an infeed unit 1, an egg lifting unit 2, a punching unit 3, a membrane-piercing unit 4, a liquid-injecting unit 5, and an outfeed unit 6 arranged sequentially along the egg tray conveying direction. The control system is connected to the infeed unit 1, egg lifting unit 2, punching unit 3, membrane-piercing unit 4, liquid-injecting unit 5, and outfeed unit 6 to control their corresponding actions. For example, the control system can control and adjust the start and stop actions and conveying speed of the infeed unit 1 and outfeed unit 6, adjust the start and stop actions and working stroke of the punching unit 3 and membrane-piercing unit 4, and control and adjust the start and stop actions and liquid injection volume of the liquid-injecting unit 5.

[0059] In this embodiment, the tray feeding unit 1 horizontally delivers the egg tray containing the embryos to the position of the egg lifting unit 2;

[0060] Egg lifting unit 2 raises the embryos in the egg tray from their initial height to the working height of perforation unit 3. The egg-lifting component remains in a lifting state throughout the process, allowing taller embryos to contact the limiting plate first. Then, as the egg-lifting component continues to rise, the egg-holding component corresponding to the taller embryo is pressed down until the shorter embryo also contacts the limiting plate. Thus, by using the egg lifting unit and its egg-lifting component, embryos of different heights are equally limited in the guide plate. Embryos of different sizes and heights can be directly placed in the egg tray without pre-grading them by height; there is no need to readjust the pressure and lifting height beforehand due to varying embryo heights.

[0061] The punching unit 3 descends to the top of the embryo and punches holes in the embryo at the current working height to facilitate the subsequent membrane insertion process.

[0062] After the perforation is completed, the membrane-piercing unit 4 descends to the top of the embryo and pierces the embryo at the current working height, so as to facilitate the subsequent delivery of the inoculation liquid into the preset position inside the embryo.

[0063] Injection unit 5 injects the inoculation liquid into a preset position inside the embryo.

[0064] After the liquid injection unit finishes injecting the liquid, the egg lifting unit 2 lowers the embryo to the position of the bottom egg tray;

[0065] The tray delivery unit 6 horizontally delivers the tray containing the injected embryos.

[0066] like Figure 5-6 As shown, in this embodiment,

[0067] The egg-lifting unit 2 includes a base plate 21 fixed to the frame. A lifting cylinder 22 is fixed to the top of the base plate 21. The output end of the lifting cylinder 22 is fixedly connected to a top plate 23. Driven by the lifting cylinder 22, the top plate 23 can move up and down relative to the base plate 21 along the guide assembly 24. The top of the top plate 23 lifts the embryos in the egg tray through the egg-lifting assembly 25. By setting the guide assembly 24, the top plate 23 can move up and down relative to the base plate 21 in a predetermined vertical direction, avoiding unexpected deviations that could adversely affect the transport of the top egg tray A. By setting the egg-lifting assembly, embryos of different heights can be equally limited in the guide plate, such as... Figure 7 As shown, embryos of different sizes and heights can be placed directly into the egg tray without prior grading of the embryos before they enter the inoculation machine; and there is no need to readjust the clamping force of the embryos beforehand because of their different heights.

[0068] like Figure 5 As shown, the guide assembly 24 in this embodiment includes a guide rod 241 fixed to the top of the base plate 21, a sleeve 242 fixedly disposed at the bottom of the top plate 23, and the top plate 23 and the sleeve 242 being sleeved on the outer periphery of the guide rod 241 in a liftable manner. A limiting member 243 extending radially along the guide rod 241 is also fixed at the top of the guide rod 241. When the top plate 23 and the sleeve 242 are sleeved on the outer periphery of the guide rod 241 and move upward relative to the base plate 21 to the position of the limiting member 243, the top of the top plate 23 will touch the bottom of the limiting member 243, thereby limiting the top plate 23 to the highest position. Preferably, the limiting effect of the top plate 23 to different highest positions can be achieved by modifying the different height positions of the limiting member 243 at the top of the guide rod 241.

[0069] like Figure 6As shown, the egg-lifting assembly 25 in this embodiment includes a pressure device 251. The pressure device 251 is located on the top of the top plate 23 and corresponds to the position of each embryo in the egg tray A. The pressure device 251 can provide different lifting pressures to each embryo at different heights, so that each embryo can be lifted by the pressure device 251 so that the top of the embryo is at the same height in the guide plate 253. Preferably, in this embodiment, the total pressure provided to all pressure devices 251 can be set to a constant value, and the lifting stroke required for each embryo at different heights is different (the lifting stroke required for higher embryos is relatively small, while the lifting stroke required for lower embryos is large). Therefore, the extra pressure at the higher embryo position can be distributed to the lower embryo position through the air pipe, thereby further improving the working efficiency of the pressure device 251. If there are many higher embryos, the total pressure can be maintained constant by depressurizing the main air path (if there are many lower embryos, a pressurization method is used).

[0070] The top of the pressure device 251 is fixedly provided with an egg-topping elastic element 252, which abuts against the bottom of each embryo in the egg tray A. The egg-topping elastic element 252 can preferably be an elastic element such as rubber. By abutting against the bottom of the embryo, the potential squeezing damage to the embryo can be minimized. A guide plate 253 is also provided on the top of the egg tray A. The internal through holes of the guide plate 253 correspond to the position of each embryo in the egg tray A. By providing the guide plate 253, the tops of embryos of different heights can be confined within the internal through holes of the guide plate 253, so that the tops of embryos of different heights are all located on the same working plane, which facilitates the subsequent overall drilling and membrane-piercing operations. As a further preferred embodiment, the internal through holes of the guide plate 253 are set as tapered holes with a smaller top and a larger bottom to match the structural shape of the top of the embryo, thereby further improving the positioning stability of the top of the embryo inside the guide plate 253.

[0071] like Figure 8 As shown, the punching unit 3 in this embodiment includes a punching cylinder 31 fixed on an external bracket 3A. Guide rods 3B are fixed on both sides of the external bracket 3A. A punching bracket 32 ​​is fixedly connected to the extended end of the punching cylinder 31. The punching bracket 32 ​​is sleeved on the outer periphery of the guide rods 3B and can slide up and down relative to the guide rods 3B. By setting a common guide rod 3B, the lifting and lowering movements of the punching bracket 32 ​​and the membrane-penetrating bracket 42 can be stably operated along a predetermined trajectory, thereby improving the stability of their lifting and lowering movements. A punching needle mounting part 33 is fixed at the bottom of the punching bracket 32. A punching needle 34 is installed inside the punching needle mounting part 33. Thus, when the punching bracket 32 ​​lifts and lowers, the punching needle 34 is driven by the punching needle mounting part 33 to perform the punching action.

[0072] The film-piercing unit 4 includes a film-piercing cylinder 41 fixed on the external bracket 3A. The extended end of the film-piercing cylinder 41 is fixedly connected to a film-piercing bracket 42. The film-piercing bracket 42 is sleeved on the outer periphery of the guide rod 3B and can slide up and down relative to the guide rod 3B. A film-piercing needle mounting part 43 is fixed at the bottom of the film-piercing bracket 42. A film-piercing needle 44 is installed inside the film-piercing needle mounting part 43. Thus, when the film-piercing bracket 42 moves up and down, the film-piercing needle 44 moves up and down synchronously through the film-piercing needle mounting part 43 to perform the film-piercing action.

[0073] As a further preferred embodiment, a punching limiting device 35 is provided inside the external bracket 3A, which can limit the lifting stroke of the punching bracket 32. A film-penetrating limiting device 45 is provided inside the punching bracket 32, which can limit the lifting stroke of the film-penetrating bracket 42. Thus, according to the punching and film-penetrating requirements, the limiting positions of the punching limiting device 35 and the film-penetrating limiting device 45 can be pre-adjusted by the control system to limit the working stroke of the punching needle 34 and the film-penetrating needle 44.

[0074] In this embodiment, the working process of the punching unit 3 is as follows: the extended end of the punching cylinder 31 extends, driving the punching bracket 32 ​​to descend along the guide rod 3B, and the punching needle 34 installed on the punching needle mounting part 33 also descends accordingly. It stops when it reaches the limit position of the punching limit device 35, thereby completing the punching operation on the top of the embryo egg.

[0075] In this embodiment, the working process of the membrane-piercing unit 4 is as follows: the extension end of the membrane-piercing cylinder 41 extends, driving the membrane-piercing bracket 42 to descend along the guide rod 3B, and the membrane-piercing needle 44 installed on the membrane-piercing needle mounting part 43 also descends accordingly. It stops when it reaches the limit position of the membrane-piercing limit device 45, thereby completing the membrane-piercing operation on the top of the embryo egg.

[0076] like Figure 8 As shown, in this embodiment, the membrane-penetrating bracket 42 is located inside the perforated bracket 32 ​​and can move up and down relative to the perforated bracket 32. This saves space while limiting the lifting and lowering process of the inner membrane-penetrating bracket 42 through the outer perforated bracket 32, improving the accuracy of the lifting and lowering movement. It also improves the accuracy of the subsequent membrane-penetrating needle 44 in the lifting and lowering movement of the perforated needle 34. The membrane-penetrating needle 44 is located inside the perforated needle 34 and can move up and down relative to the perforated needle 34. This saves space while limiting the lifting and lowering process of the inner membrane-penetrating needle 34 through the outer perforated needle 34.

[0077] like Figure 9-10As shown, the injection unit 5 in this embodiment includes a storage tank 51 containing inoculation liquid. One end of the storage tank 51 is connected to a collection pipe 53 via a valve 52. Several connectors 54 are evenly distributed along the outer edge of the collection pipe 53. Each connector 54 is connected to a perforation needle 44 at the bottom via a pipe 56 and an adapter 57. The valve can be a positive pressure regulating valve. When the valve is open, purified positive pressure gas drives the inoculation liquid in the storage tank 51 to flow into the collection pipe 53. The purified positive pressure gas then drives the inoculation liquid from the connector 54 through the pipe 56 and adapter 57 to the perforation needle 44 at the bottom. The positive pressure introduced into the storage tank 51 when the valve is open drives the inoculation liquid in the storage tank 51 to flow out. The valve can reduce the input positive pressure to a stable pressure to drive the inoculation liquid out of the storage tank 51. The flow rate of the inoculation liquid can be adjusted by regulating the magnitude of this stable positive pressure. To ensure the cleanliness of the pressurized air source entering the storage tank, a sterile filter can be installed upstream of the valve.

[0078] Preferably, pipe 56 can be opened and closed as needed, such as... Figure 9 As shown, in this embodiment, the pipe 56 is selectively connected to the adapter 57 and the bottom membrane needle 44 via the opening and closing controller 55. The opening and closing controller 55 includes a control cylinder 551 fixed to the pipe frame 58. The cylinder shaft 552 of the control cylinder can extend towards and squeeze the pipe 56, or retract away from and release the pipe 56. Thus, by controlling the extension and retraction of the cylinder shaft 552 of the control cylinder 551, the pipe 56 is squeezed or released, thereby realizing the opening and closing control of the pipe 56. An elastic element 553 is also sleeved on the outer periphery of the cylinder shaft 552. The two ends of the elastic element 553 are fixedly connected to the control cylinder 551 and the cylinder shaft 552 respectively. The elastic element 553 can help to provide assistance in the process of closing the pipe when the cylinder shaft 552 extends. At the same time, it can also keep the liquid pipe closed by pressing when the device closes unexpectedly or the cylinder fails.

[0079] As one preferred embodiment, multiple pipes 56 can be arranged in parallel on a fixed plate, and the switching function of opening and closing the pipes 56 can be performed by a single cylinder shaft 552, which saves time and effort while improving the timeliness, synchronization and stability of the opening and closing control.

[0080] In this embodiment, the specific working process of the injection unit 5 is as follows: When the punching needle 34 penetrates the top of the eggshell and the membrane-piercing needle 44 pierces the air chamber membrane, the cylinder shaft 552 retracts, causing it to move away from the pipe 56, thereby opening the pipe 56. The liquid in the storage tank 51 enters the collection pipe 53 under the pressure of the positive valve opening, and then enters the corresponding pipe 56 through several joints 54 on the collection pipe 53. It then enters the corresponding adapter 57 and its bottom membrane-piercing needle through the pipe 56, allowing the inoculation liquid to enter the preset part of the egg through several membrane-piercing needles. When the opening time of the cylinder shaft 552 reaches the preset time, the cylinder shaft 552 extends, moving closer to the pipe 56 and squeezing the pipe 56 to close it.

[0081] [Second Embodiment]

[0082] The second embodiment of this utility model also provides a control method for the device for injecting liquid into embryos mentioned in the first embodiment, comprising the following steps:

[0083] S1: The infeeding unit 1 horizontally delivers the egg tray containing the embryos to the egg lifting unit 2;

[0084] S2: Egg lifting unit 2 lifts the embryo eggs in the egg tray at the current position to the working height of punching unit 3;

[0085] S3: The egg-lifting assembly lifts the embryos in the egg tray into the guide plate;

[0086] S4: Drilling unit 3 performs a drilling action on the top of the embryo that is higher than the guide plate;

[0087] S5: After the perforation is completed, the membrane-piercing unit 4 performs the membrane-piercing action on the top of the embryo that is higher than the guide plate;

[0088] S6: Open the valve and the on / off controller, and the inoculation liquid in the storage tank 51 is injected into the preset position inside the embryo through the collection pipe 53 and the pipeline 56;

[0089] S7: After injection, close the valve and the on / off controller;

[0090] S8: The egg-top component descends, dropping the embryo back into the bottom egg tray;

[0091] S9: Egg lifting unit 2 lowers the embryonic egg in the egg tray to the initial height;

[0092] S10: Dispensing unit 6 horizontally delivers the egg tray containing the embryos.

Claims

1. A device for injecting liquid into embryos, characterized in that, The system includes an infeed unit (1), an egg lifting unit (2), a punching unit (3), a membrane-piercing unit (4), a liquid injection unit (5), and an outfeed unit (6) arranged sequentially along the egg tray conveying direction. The control system is connected to the infeed unit (1), the egg lifting unit (2), the punching unit (3), the membrane-piercing unit (4), the liquid injection unit (5), and the outfeed unit (6) respectively to control them to perform corresponding actions. The tray feeding unit (1) horizontally delivers the egg tray containing the embryos to the egg lifting unit (2); The egg-lifting unit (2) lifts the embryo eggs in the egg tray from the initial height to the working height of the punching unit (3); The punching unit (3) descends to the top of the embryo and punches holes in the embryo at the current working height. After the punching is completed, the membrane-piercing unit (4) descends to the top of the embryo to pierce the embryo at the current working height. The injection unit (5) injects the inoculation liquid into a preset position inside the embryo. After the liquid injection unit finishes injecting the liquid, the egg lifting unit (2) lowers the embryo to the position of the bottom egg tray; The tray delivery unit (6) horizontally delivers the tray containing the injected embryos.

2. The device for injecting liquid into an embryo according to claim 1, characterized in that: The egg-lifting unit (2) includes a base plate (21) fixed to the frame. A lifting cylinder (22) is fixed on the top of the base plate (21). The output end of the lifting cylinder (22) is fixedly connected to the top plate (23). The top plate (23) can move up and down relative to the base plate (21) along the guide assembly (24) under the drive of the lifting cylinder (22). The top of the top plate (23) lifts the embryo eggs in the egg tray through the egg-lifting assembly (25). The guide assembly (24) includes a guide rod (241) fixed to the top of the base plate (21), a sleeve (242) fixedly provided at the bottom of the top plate (23), the top plate (23) and the sleeve (242) being sleeved on the outer periphery of the guide rod (241) in a liftable manner, and a limiting member (243) extending radially along the guide rod (241) is also fixed at the top of the guide rod (241). The egg-topping assembly (25) includes a pressure device (251), which is located on the top of the top plate (23) and corresponds to the position of each embryo in the egg tray. The top protruding end of the pressure device (251) is fixedly provided with an egg-topping elastic element (252), which abuts against the bottom of each embryo in the egg tray. A guide plate (253) is also provided on the top of the egg tray, and the internal through holes of the guide plate (253) correspond to the position of each embryo in the egg tray.

3. The device for injecting liquid into an embryo according to claim 1, characterized in that: The punching unit (3) includes a punching cylinder (31) fixed on an external bracket (3A). Guide rods (3B) are fixed on both sides of the external bracket (3A). A punching bracket (32) is fixedly connected to the extended end of the punching cylinder (31). The punching bracket (32) is sleeved on the outer periphery of the guide rod (3B) and can slide up and down relative to the guide rod (3B). A punching needle mounting part (33) is fixed at the bottom of the punching bracket (32). A punching needle (34) is installed inside the punching needle mounting part (33).

4. The apparatus for injecting liquid into an embryo according to claim 3, characterized in that: The membrane insertion unit (4) includes a membrane insertion cylinder (41) fixed on an external bracket (3A). The extended end of the membrane insertion cylinder (41) is fixedly connected to a membrane insertion bracket (42). The membrane insertion bracket (42) is sleeved on the outer periphery of the guide rod (3B) and can slide up and down relative to the guide rod (3B). A membrane insertion needle mounting part (43) is fixed at the bottom of the membrane insertion bracket (42). A membrane insertion needle (44) is installed inside the membrane insertion needle mounting part (43).

5. The apparatus for injecting liquid into an embryo according to claim 3, characterized in that: The external bracket (3A) is provided with a punching limit device (35) inside, which can limit the lifting stroke of the punching bracket (32).

6. The apparatus for injecting liquid into an embryo according to claim 4, characterized in that: The perforated bracket (32) is provided with a membrane-penetrating limiting device (45) inside, which can limit the lifting stroke of the membrane-penetrating bracket (42).

7. The apparatus for injecting liquid into an embryo according to claim 4, characterized in that: The membrane support (42) is located inside the perforated support (32) and can move up and down relative to the perforated support (32). The membrane needle (44) is located inside the perforated needle (34) and can move up and down relative to the perforated needle (34).

8. The apparatus for injecting liquid into an embryo according to claim 4, characterized in that: The injection unit (5) includes a storage tank (51) containing inoculation liquid. One end of the storage tank (51) is connected to the collection pipe (53) through a valve (52). Several connectors (54) are evenly distributed on the outer edge of the collection pipe (53). The connectors (54) are connected to the membrane needle (44) at the bottom through a pipe (56) and an adapter (57).

9. The apparatus for injecting liquid into an embryo according to claim 8, characterized in that: The pipeline is selectively connected to the adapter (57) and the bottom piercing needle (44) via the opening and closing controller (55). The opening and closing controller (55) includes a control cylinder (551) fixed to the pipeline frame (58). The cylinder shaft (552) of the control cylinder can extend towards and squeeze the pipeline (56) or retract away from and release the pipeline (56). An elastic element (553) is also sleeved on the outer periphery of the cylinder shaft (552). The two ends of the elastic element (553) are fixedly connected to the control cylinder (551) and the cylinder shaft (552) respectively.

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