Chromatographic system

Abstract

The utility model discloses a chromatography system which comprises a sample container, a buffer solution container, a chromatographic column, a main pipeline, a circulating pipeline and a recovery pipeline, one end of the main pipeline is respectively communicated with the sample container and the buffer solution container, and the other end of the main pipeline is respectively communicated with one end of the chromatographic column and one end of the circulating pipeline. The other end of the circulating pipeline is respectively communicated with the sample container and the buffer solution container, and the recovery pipeline is communicated with the two ends of the circulating pipeline. The chromatography system has the advantages of reducing waste, lowering production cost and the like.

Description

Technical Field

[0001] This utility model relates to the field of food and pharmaceutical packaging machinery and equipment technology, and specifically to a chromatography system. Background Technology

[0002] Existing chromatography systems generally include a tubing consisting of multiple pipes and a chromatography column connected to the tubing. Before chromatography, it is generally necessary to remove air bubbles from the buffer solution and sample (hereinafter collectively referred to as solution) through the tubing. After the air bubbles are removed, solution residue is easily left in the tubing, resulting in waste and increasing production costs.

[0003] There are two common methods for removing air bubbles from chromatography channels. The first method involves the solution first entering a container-like bubble trap and then flowing out of it to remove air bubbles from the chromatography channel. The disadvantage of this method is that the bubble trap needs to be filled with buffer solution before sample loading. Since the volume of the bubble trap is much larger than the volume of the chromatography system's tubing, the sample will be diluted after entering the trap. Excessive dilution can affect the chromatographic peak shape and the detection of lower-content components in the sample. The second method involves direct effluent removal of the solution from the inlet channel, with the air bubbles being discharged along with the solution. This method requires the discharge of a certain volume of sample, resulting in some solution waste. When the solution is of high value, this increases production costs. Furthermore, the need to prepare a larger volume of solution necessitates larger storage containers, increasing the manufacturing cost of the equipment. Utility Model Content

[0004] The technical problem to be solved by this invention is to overcome the shortcomings of the prior art and provide a chromatography system that reduces waste and lowers production costs.

[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0006] A chromatography system includes a sample container, a buffer container, a chromatography column, a main pipeline, a circulation pipeline, and a recovery pipeline. One end of the main pipeline is connected to the sample container and the buffer container, respectively. The other end of the main pipeline is connected to one end of the chromatography column and one end of the circulation pipeline, respectively. The other end of the circulation pipeline is connected to the sample container and the buffer container, respectively. The recovery pipeline connects the two ends of the circulation pipeline.

[0007] As a further improvement to the above technical solution:

[0008] The other end of the circulation pipe is also connected to an air intake assembly.

[0009] The air intake assembly includes an air intake pipe, a seventh valve, and a gas filter. The air intake pipe is connected to the circulation pipe, and the seventh valve and the gas filter are both located on the air intake pipe.

[0010] The main pipeline is connected to the sample container via a first valve, to the buffer container via a second valve, to the chromatography column via a third valve, and to the circulation pipeline via a fourth valve. The circulation pipeline is connected to the sample container and the recovery pipeline via a fifth valve, and to the buffer container via a sixth valve. A chromatography pump is installed on the main pipeline, and a recovery valve is installed on the recovery pipeline.

[0011] There are two recovery valves, located at both ends of the recovery pipeline.

[0012] The circulation pipeline includes a height difference section.

[0013] The circulation pipeline also includes a variable diameter section with an inner diameter smaller than that of the main pipeline.

[0014] The main pipeline is equipped with a bubble detection device, and the circulation pipeline is equipped with a liquid level detection device.

[0015] The outlet end of the chromatography column is connected to a collection container via a collection tube, and a collection valve is provided on the collection tube.

[0016] The outlet end of the chromatography column is also connected to a discharge pipe, and a discharge valve is provided on the discharge pipe.

[0017] Compared with the prior art, the advantages of this utility model are:

[0018] In this chromatography system, the sample in the sample container flows through a circulation loop consisting of the sample container, main pipe, and circulation pipe, thus removing air bubbles from the sample. Similarly, the buffer solution in the buffer solution container flows through a circulation loop consisting of the buffer solution container, main pipe, and circulation pipe, thus removing air bubbles from the buffer solution. After the air bubbles are removed, the residual solution in the circulation pipe can be recovered to the sample container or buffer solution container through the recovery pipe under the action of external airflow, reducing waste and lowering production costs. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the chromatography system of this utility model.

[0020] Figure 2 This is a schematic diagram illustrating the sample degassing principle of the chromatography system of this invention.

[0021] Figure 3 This is a schematic diagram of the buffer degassing principle of the chromatography system of this utility model.

[0022] Figure 4This is a schematic diagram of the sample recovery principle in the circulation pipeline of the chromatography system of this utility model.

[0023] Figure 5 This is a schematic diagram of the sample recovery principle in the main pipeline of the chromatography system of this utility model.

[0024] The labels in the diagram represent:

[0025] 1. Sample container; 2. Buffer solution container; 3. Chromatography column; 31. Collection tube; 32. Collection valve; 33. Discharge tube; 34. Discharge valve; 4. Main pipeline; 41. First valve; 42. Second valve; 43. Third valve; 44. Fourth valve; 45. Bubble detection device; 5. Circulation pipeline; 51. Fifth valve; 52. Sixth valve; 53. Height difference section; 54. Seventh valve; 55. Liquid level detection device; 6. Chromatography pump; 7. Gas filter; 71. Inlet pipeline; 8. Recovery pipeline; 81. Recovery valve; 9. Collection container. Detailed Implementation

[0026] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0027] In the description of this utility model, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0028] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0029] In this utility model, unless otherwise explicitly specified and limited, the terms "assembly," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0030] Figures 1 to 5 An embodiment of the chromatography system of this utility model is shown. The chromatography system of this embodiment includes a sample container 1, a buffer container 2, a chromatography column 3, a main pipeline 4, a circulation pipeline 5, and a recovery pipeline 8. One end of the main pipeline 4 is connected to the sample container 1 and the buffer container 2 respectively. The other end of the main pipeline 4 is connected to one end of the chromatography column 3 and the circulation pipeline 5 respectively. The other end of the circulation pipeline 5 is connected to the sample container 1 and the buffer container 2 respectively. The recovery pipeline 8 connects the two ends of the circulation pipeline 5.

[0031] In this chromatography system, the sample in sample container 1 flows through a circulation loop consisting of sample container 1, main pipe 4, and circulation pipe 5, thus removing air bubbles from the sample. Similarly, the buffer solution in buffer container 2 flows through a circulation loop consisting of buffer container 2, main pipe 4, and circulation pipe 5, thus removing air bubbles from the buffer solution. After air bubble removal, the residual solution in circulation pipe 5 can be recovered to sample container 1 or buffer container 2 through recovery pipe 8 under the action of external airflow, reducing waste and lowering production costs.

[0032] Furthermore, in this embodiment, the other end of the circulation pipe 5 is also connected to an air intake assembly, which is used to provide external airflow into the circulation pipe 5.

[0033] Furthermore, in this embodiment, the air intake assembly includes an air intake pipe 71, a seventh valve 54, and a gas filter 7. The air intake pipe 71 is connected to the circulation pipe 5, and the seventh valve 54 and the gas filter 7 are both located on the air intake pipe 71.

[0034] Furthermore, in this embodiment, the main pipeline 4 is connected to the sample container 1 via the first valve 41, to the buffer container 2 via the second valve 42, to the chromatography column 3 via the third valve 43, and to the circulation pipeline 5 via the fourth valve 44. The circulation pipeline 5 is connected to the sample container 1 and the recovery pipeline 8 via the fifth valve 51, and to the buffer container 2 via the sixth valve 52. The main pipeline 4 is equipped with a chromatography pump 6, and the recovery pipeline 8 is equipped with a recovery valve 81.

[0035] like Figure 2 As shown, the sample degassing process is as follows: Second valve 42, recovery valve 81, sixth valve 52, seventh valve 54, and third valve 43 are closed; first valve 41, fourth valve 44, and fifth valve 51 are opened. Chromatography pump 6 is turned on. Under the action of chromatography pump 6, the sample circulates through the loop consisting of sample container 1, main pipe 4, and circulation pipe 5, thus removing air bubbles from the sample. Circulation pipe 5 is the degassing pipe.

[0036] Furthermore, in this embodiment, the circulation pipe 5 includes an elevation difference section 53 with a height difference. Since the bubble density is lower than that of the sample, the bubbles can be discharged from the pipe more smoothly during the upward flow in the elevation difference section 53.

[0037] Furthermore, in this embodiment, the circulation pipe 5 also includes a reducing section with an inner diameter smaller than that of the main pipe 4. Due to the presence of the pipe reducing diameter, the flow rate of the sample in the circulation pipe 5 increases, which is beneficial for the precipitation of bubbles in the sample.

[0038] like Figure 3 As shown, the buffer degassing process is as follows: First valve 41, recovery valve 81, fifth valve 51, seventh valve 54, and third valve 43 are closed; second valve 42, fourth valve 44, and sixth valve 52 are opened; chromatography pump 6 is turned on. Under the action of chromatography pump 6, the buffer solution circulates through the circulation loop composed of buffer solution container 2, main pipeline 4, and circulation pipeline 5, thereby removing bubbles from the buffer solution.

[0039] like Figure 4 As shown, the sample recovery process in the circulation pipeline 5 is as follows: the first valve 41, the second valve 42, the fourth valve 44, the fifth valve 51, and the sixth valve 52 are closed, while the recovery valve 81 and the seventh valve 54 are opened; the air inlet pipeline 71 is connected to the external air supply mechanism; under the action of the air supply mechanism, the residual sample in the circulation pipeline 5 is recovered into the sample container 1 through the recovery pipeline 8.

[0040] like Figure 5 As shown, the sample recovery process in the main pipeline 4 is as follows: the second valve 42, the third valve 43, the recovery valve 81, the fifth valve 51, and the sixth valve 52 are closed, while the first valve 41, the fourth valve 44, and the seventh valve 54 are opened; the air inlet pipeline 71 is connected to the external air supply mechanism; under the action of the air supply mechanism, the residual samples in the circulation pipeline 5 and the main pipeline 4 are recovered into the sample container 1.

[0041] Furthermore, in this embodiment, two recovery valves 81 are provided, located at both ends of the recovery pipeline 8 respectively. During the degassing process, the recovery valves 81 at both ends of the recovery pipeline 8 can be closed to prevent samples and buffer solutions from entering the recovery pipeline 8.

[0042] Furthermore, in this embodiment, the main pipe 4 is equipped with a bubble detection element 45, and the circulation pipe 5 is equipped with a liquid level detection element 55. During the sample recovery process, the liquid level detection element 55 can detect whether there is liquid in the circulation pipe 5, thereby detecting whether the recovery is complete.

[0043] When the chromatography pump 6 is turned on, the sample flows through the circulation loop consisting of the sample container 1, the main pipeline 4, and the circulation pipeline 5 under the action of the chromatography pump 6, which can remove air bubbles from the sample. When the air bubble detector 45 does not detect any air bubbles, it means that the air bubble removal operation of the pipeline is completed.

[0044] Furthermore, in this embodiment, the outlet end of the chromatography column 3 is connected to a collection container 9 via a collection pipe 31, and a collection valve 32 is provided on the collection pipe 31.

[0045] Furthermore, in this embodiment, the outlet end of the chromatography column 3 is also connected to a discharge pipe 33, and a discharge valve 34 is provided on the discharge pipe 33.

[0046] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make many possible variations and modifications to the present invention, or modify it into equivalent embodiments, without departing from the scope of the present invention. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention, without departing from the content of the present invention, should fall within the protection scope of the present invention.

Claims

1. A chromatography system, characterized in that: It includes a sample container (1), a buffer container (2), a chromatography column (3), a main pipeline (4), a circulation pipeline (5), and a recovery pipeline (8). One end of the main pipeline (4) is connected to the sample container (1) and the buffer container (2), respectively. The other end of the main pipeline (4) is connected to one end of the chromatography column (3) and the circulation pipeline (5), respectively. The other end of the circulation pipeline (5) is connected to the sample container (1) and the buffer container (2), respectively. The recovery pipeline (8) is connected to both ends of the circulation pipeline (5).

2. The chromatography system according to claim 1, characterized in that: The other end of the circulation pipe (5) is also connected to an air intake assembly.

3. The chromatography system according to claim 2, characterized in that: The air intake assembly includes an air intake pipe (71), a seventh valve (54), and a gas filter (7). The air intake pipe (71) is connected to the circulation pipe (5), and the seventh valve (54) and the gas filter (7) are both located on the air intake pipe (71).

4. The chromatography system according to claim 2, characterized in that: The main pipeline (4) is connected to the sample container (1) through the first valve (41), to the buffer container (2) through the second valve (42), to the chromatography column (3) through the third valve (43), and to the circulation pipeline (5) through the fourth valve (44). The circulation pipeline (5) is connected to the sample container (1) and the recovery pipeline (8) through the fifth valve (51) and to the buffer container (2) through the sixth valve (52). The main pipeline (4) is equipped with a chromatography pump (6), and the recovery pipeline (8) is equipped with a recovery valve (81).

5. The chromatography system according to claim 4, characterized in that: Two recovery valves (81) are provided, located at both ends of the recovery pipeline (8).

6. The chromatography system according to claim 1, characterized in that: The circulation pipe (5) includes a height difference section (53) with a height difference.

7. The chromatography system according to claim 1, characterized in that: The circulating pipeline (5) also includes a variable diameter section with an inner diameter smaller than that of the main pipeline (4).

8. The chromatography system according to claim 1, characterized in that: The main pipe (4) is equipped with a bubble detection element (45), and the circulation pipe (5) is equipped with a liquid level detection element (55).

9. The chromatography system according to any one of claims 1 to 8, characterized in that: The outlet end of the chromatography column (3) is connected to a collection container (9) via a collection tube (31), and a collection valve (32) is provided on the collection tube (31).

10. The chromatography system according to claim 9, characterized in that: The outlet end of the chromatography column (3) is also connected to a discharge pipe (33), and a discharge valve (34) is provided on the discharge pipe (33).

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