Polypeptide separation device based on membrane separation technology
By using ceramic membranes and special separation membranes for fractional separation in a membrane separation device, combined with biological deodorizers and reciprocating components, the problems of low fractional separation efficiency and odor impact in existing technologies are solved, achieving efficient and rapid peptide separation and high purity.
Patent Information
- Application Number
- CN202422815986.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-19
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2034-11-19
AI Technical Summary
Existing membrane separation devices cannot achieve fractional separation, have slow separation speed and low efficiency, and the soybean protein hydrolysate has an off-odor, which affects the separation efficiency and peptide purity.
A peptide separation device based on membrane separation technology is designed. It uses ceramic membranes and special separation membranes to achieve graded separation, combined with a closed feeding mechanism and reciprocating components, and removes odors with a biological deodorizer to improve separation speed and purity.
It achieves graded separation of peptides, improves separation speed and efficiency, and enhances peptide purity by removing odors.
Smart Images
Figure CN223832113U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of membrane separation technology, specifically to a peptide separation device based on membrane separation technology. Background Technology
[0002] Soybean peptides are a mixture of low-molecular-weight peptides obtained by enzymatic decomposition of soybean protein as the basic raw material. They possess advantages such as hydrophilicity, high solubility, thermal stability, and low viscosity, resulting in better absorption than amino acids. Furthermore, many low-molecular-weight peptides also have certain physiological and health-promoting functions. Developing soybean peptide products is of great significance and economic value for promoting the development of my country's soybean deep-processing industry and enhancing the added value and technological content of soybean products.
[0003] Currently, the main components of soybean protein enzymatic hydrolysates include proteins, mixed peptides, and amino acids. Traditional separation methods include reversed-phase high-performance liquid chromatography (RP-HPLC), gel filtration chromatography, ion exchange chromatography, electrophoresis, affinity chromatography, and aqueous two-phase extraction, but these methods suffer from high costs, complex operations, and difficulties in large-scale production. Membrane technology, as a novel separation technology, overcomes many limitations of traditional methods due to its advantages such as no phase change, no secondary pollution, biofilm concentration and enrichment capabilities, ease of operation, compact structure, low maintenance costs, and ease of automation.
[0004] However, there are still some problems with membrane separation technology at present; (1) Existing membrane separation devices usually use a single membrane element, which cannot achieve graded separation; (2) Existing membrane separation devices often use static separation, resulting in slow separation speed and low efficiency; (3) Soybean protein hydrolysate often has an off-odor, which affects the subsequent separation efficiency and peptide purity; In view of this, this utility model designs a peptide separation device based on membrane separation technology, which aims to solve the above problems. Summary of the Invention
[0005] (a) Technical problems to be solved
[0006] To address the shortcomings of existing technologies, this invention provides a peptide separation device based on membrane separation technology, which has the advantages of fractional separation, fast separation speed, high efficiency, and high purity, and solves the defects of poor separation effect, low efficiency, and low purity of existing peptide separation devices based on membrane separation technology.
[0007] (II) Technical Solution
[0008] To achieve the above objectives, this utility model provides the following technical solution: a peptide separation device based on membrane separation technology, comprising a base support and a reciprocating assembly disposed above the base support. A separation mechanism is connected to the top of the reciprocating assembly. This separation mechanism, from top to bottom, includes a closed feeding mechanism, a filter screen, a first separation system, a second separation system, and an inclined plate. A baffle ring is disposed below the filter screen, and a cover plate is disposed on top of the separation mechanism. The mixed peptide solution enters through the closed feeding mechanism, where larger impurities are separated by the filter screen. It then passes through the first and second separation systems, which use membranes with different pore sizes to achieve graded separation of the peptides. The reciprocating assembly drives the separation mechanism in reciprocating motion, improving the separation speed and efficiency. The device features a closed feeding mechanism and uses a biological deodorizer to adsorb and treat the peptide solution, reducing odor and improving peptide purity and separation efficiency.
[0009] Preferably, the sealed feeding mechanism includes a feed inlet, a sealed hood, and a biological deodorizer; a trumpet-shaped sealed hood is vertically attached and fixed to the lower surface of the cover plate relative to the left outer side of the separation mechanism, the left end of the sealed hood is a small diameter end and the right end is a large diameter end, the small diameter end is sealed and connected to the feed inlet, and the biological deodorizer is placed in the middle of the sealed hood; the mixed polypeptide liquid enters the sealed hood through the feed inlet, and after the odor is removed by the biological deodorizer, it flows into the filter screen for filtration; a level gauge is also provided on the right inner side wall of the separation mechanism, and the level gauge is located slightly below the horizontal plane where the large diameter end of the sealed hood is located.
[0010] Preferably, the filter screen is made of stainless steel.
[0011] Preferably, the first separation system includes a first inlet pipe, a primary pump, a ceramic membrane, a first outlet, and a first solenoid valve. The ceramic membrane is vertically spaced below the filter screen. The first outlet is also inclined on the right outer side of the separation mechanism relative to the position of the ceramic membrane. The left end of the first outlet extends obliquely upward to the plane where the lower edge of the ceramic membrane is located. A first solenoid valve for controlling its opening and closing is also provided on the first outlet. The pore size of the ceramic membrane is 200 nm, which can retain polypeptide molecules with a pore size of 10000 Da or more. The polypeptide molecules that pass through the ceramic membrane are transported to the second separation system by the secondary pump, while the polypeptide molecules that cannot pass through the ceramic membrane are collected and discharged through the first outlet.
[0012] Preferably, the second separation system includes a second inlet pipe, a secondary pump, a special separation membrane, a second outlet, a second solenoid valve, an inclined plate, a third outlet, and a third solenoid valve; a matching special separation membrane and an inclined plate are also provided at intervals below the first separation system, the special separation membrane being horizontally arranged within the separation mechanism; a second outlet is also inclined on the right outer side of the separation mechanism relative to the position of the special separation membrane, the left end of the second outlet extending obliquely upward into the separation mechanism, and the upper end of the second outlet near its lower edge being located at the right end of the upper surface of the special separation membrane, and a second solenoid valve controlling its on / off state is also provided on the second outlet; the inclined plate faces the separator. The structure is horizontally inclined downwards on the right side, and a third drain port is inclined on the right outer side of the separation mechanism relative to the inclined plate. The left end of the third drain port extends upwards into the separation mechanism, and the upper end of the third drain port is located at the lower end of the upper surface of the inclined plate near its lower edge. A third solenoid valve for controlling its opening and closing is also provided on the third drain port. The mixed polypeptide liquid that has passed through the first separation system is transported to a special separation membrane for secondary separation by a secondary pump. The special separation membrane can retain a molecular weight of 2000 Da, thereby collecting the permeable polypeptide molecules on the inclined plate and discharging them through the third drain port, while the non-permeable polypeptide molecules are discharged through the second drain port.
[0013] Preferably, the reciprocating assembly includes an outer frame, the bottom of which slides in contact with the top of the base bracket, the top of which is fixedly connected to the bottom of the separation mechanism, and meshing blocks are fixedly connected to the upper and lower inner walls of the outer frame. The meshing blocks are meshed with gears, and the gears are connected to a motor via a rotating shaft.
[0014] Preferably, the base support is provided with a sliding groove mechanism on both sides of the top, and a slider mechanism is provided in the sliding groove mechanism. A support leg is fixedly connected to the top of the slider mechanism, and the top of the support leg is fixedly connected to the bottom of the separation mechanism.
[0015] Preferably, a control mechanism is provided on the left side of the separation mechanism, and the control mechanism controls the connected motor, primary pump, and secondary pump.
[0016] (III) Beneficial Effects
[0017] Compared with the prior art, this utility model provides a peptide separation device based on membrane separation technology, which has the following beneficial effects:
[0018] 1. This peptide separation device based on membrane separation technology achieves fractional separation of mixed peptide solutions by setting ceramic membranes and special separation membranes with different molecular weight cutoffs for the two types of membranes.
[0019] 2. This peptide separation device based on membrane separation technology, by setting up a reciprocating component, drives the separation mechanism to reciprocate, and the solution inside the separation mechanism shakes, which can accelerate the separation speed;
[0020] 3. This peptide separation device based on membrane separation technology removes the odor of the mixed peptide liquid entering the separation unit by setting a closed feeding mechanism, thereby improving peptide purity and separation efficiency. Attached Figure Description
[0021] Figure 1 This is a schematic diagram of the structure of this utility model;
[0022] Figure 2 This is a schematic diagram of the closed feeding mechanism of this utility model;
[0023] Figure 3 This is a schematic diagram of the reciprocating component structure of this utility model.
[0024] In the diagram: 1. Closed feeding mechanism; 2. Filter screen; 3. Baffle ring; 4. Control mechanism; 5. Second inlet pipe; 6. Secondary pump; 7. Special separation membrane; 8. Inclined plate; 9. Reciprocating assembly; 10. Slide mechanism; 11. Sliding block mechanism; 12. Base bracket; 13. Support leg; 14. Third drain port; 15. Separation mechanism; 16. Second drain port; 17. First drain port; 18. Ceramic membrane; 19. Primary pump; 20. First inlet pipe; 21. Level gauge; 22. Cover plate; 23. Feed inlet; 24. Sealed cover; 25. Biological deodorizer; 91. Outer frame; 92. Gear; 93. Meshing block; 94. Rotating shaft Detailed Implementation
[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0026] Please see Figure 1-3 In this embodiment of the present invention, a peptide separation device based on membrane separation technology includes a base support 12 and a reciprocating assembly 9 disposed above the base support 12. A separation mechanism 15 is connected to the top of the reciprocating assembly 9. The separation mechanism 15 is provided with a sealed feeding mechanism 1, a filter screen 2, a first separation system, a second separation system and an inclined plate 8 from top to bottom. A retaining ring 3 is provided below the filter screen 2 and a cover plate 22 is provided on the top of the separation mechanism 15.
[0027] Furthermore, the sealed feeding mechanism 1 includes a feed inlet 23, a sealed cover 24, and a biological deodorizer 25; a trumpet-shaped sealed cover 24 is vertically attached and fixed to the lower surface of the cover plate 22 relative to the left outer side of the separation mechanism 15. The left end of the sealed cover 24 is a small diameter end, and the right end is a large diameter end. The small diameter end is sealed and connected to the feed inlet 23, and the biological deodorizer 25 is disposed in the middle of the sealed cover 24; the mixed polypeptide liquid enters the sealed cover 24 through the feed inlet 23, and after the odor is removed by the biological deodorizer 25, it flows into the filter screen 2 for filtration; a level gauge 21 is also provided on the right inner side wall of the separation mechanism 15. The level gauge 21 is located slightly below the horizontal plane where the large diameter end of the sealed cover 24 is located, and the level gauge 21 is used to monitor the amount of mixed polypeptide liquid entering to prevent backflow of the feed liquid.
[0028] Furthermore, the filter screen 2 is made of stainless steel.
[0029] Furthermore, the first separation system includes a first inlet pipe 20, a primary pump 19, a ceramic membrane 18, a first drain port 17, and a first solenoid valve. The ceramic membrane 18 is vertically spaced below the filter screen 2. The first drain port 17 is also inclinedly provided on the right outer side of the separation mechanism 15 relative to the position of the ceramic membrane 18. The left end of the first drain port 17 extends obliquely upward to the plane where the lower edge of the ceramic membrane 18 is located. A first solenoid valve is also provided on the first drain port 17 to control its opening and closing. The pore size of the ceramic membrane 18 is 200nm, which can retain polypeptide molecules with a pore size of 10000Da or more. The polypeptide molecules that pass through the ceramic membrane 18 are transported to the second separation system by the secondary pump 6, while the polypeptide molecules that cannot pass through the ceramic membrane 18 are collected and discharged through the first drain port 17.
[0030] Further, the second separation system includes a second inlet pipe 5, a secondary pump 6, a special separation membrane 7, a second outlet 16, a second solenoid valve, an inclined plate 8, a third outlet 14, and a third solenoid valve; below the first separation system, a matching special separation membrane 7 and an inclined plate 8 are also provided at intervals, the special separation membrane 7 being horizontally arranged within the separation mechanism 15; on the right outer side of the separation mechanism 15, relative to the position of the special separation membrane 7, a second outlet 16 is also inclined, the left end of the second outlet 16 extending obliquely upward into the separation mechanism 15, and the upper end of the second outlet 16 near its lower edge is located at the right end of the upper surface of the special separation membrane 7, and a second solenoid valve controlling its on / off state is also provided on the second outlet 16; the inclined plate 8 faces the separation... The separation mechanism 15 is horizontally inclined downwards to the right side, and a third drain port 14 is inclined on the right outer side of the separation mechanism 15 relative to the inclined plate 8. The left end of the third drain port 14 extends upwards into the separation mechanism 15, and the upper end of the third drain port 14 is located at the lower end of the upper surface of the inclined plate 8 near its lower edge. A third solenoid valve for controlling its opening and closing is also provided on the third drain port 14. The mixed polypeptide liquid that has passed through the first separation system is transported to the special separation membrane 7 by the secondary pump 6 for secondary separation. The special separation membrane 7 can retain a molecular weight of 2000 Da, thereby collecting the permeable polypeptide molecules onto the inclined plate 8 and discharging them through the third drain port 14, while the non-permeable polypeptide molecules are discharged through the second drain port 16.
[0031] Furthermore, the reciprocating assembly 9 includes an outer frame 91, the bottom of which is in sliding contact with the top of the base bracket 12, the top of which is fixedly connected to the bottom of the separation mechanism 15, and meshing blocks 93 are fixedly connected to the upper and lower inner walls of the outer frame 91. The meshing blocks 93 are meshed with gears 92, and the gears 92 are connected to the motor through a rotating shaft 94.
[0032] Furthermore, the base support 12 is provided with a sliding groove mechanism 10 on both sides of the top, and a slider mechanism 11 is provided in the sliding groove mechanism 10. A support leg 13 is fixedly connected to the top of the slider mechanism 11, and the top of the support leg 13 is connected to the bottom of the separation mechanism 15.
[0033] Furthermore, a control mechanism 4 is provided on the left side of the separation mechanism 15, and the control mechanism 4 controls the connected motor, primary pump 19, and secondary pump 6.
[0034] The working principle of this invention is as follows: The mixed polypeptide solution enters the sealed enclosure 24 through the feed inlet 23. After the odor is removed by the biological deodorizer 25, it enters the filter screen 2 for coarse filtration, where larger impurities in the mixed solution can be separated. The solution that passes through the filter screen 2 undergoes primary separation in the first separation system. Polypeptide molecules that can pass through the ceramic membrane 18 are transported to the second separation system by the secondary pump 6, while polypeptide molecules that cannot pass through are collected and discharged through the first drain port 16. The solution entering the second separation system undergoes secondary separation through the special separation membrane 7, whereby the polypeptide molecules that can pass through are collected on the inclined plate 8 and discharged through the third drain port 14, while the polypeptide molecules that cannot pass through are discharged through the second drain port 16. Meanwhile, the motor drives the gear 92 to rotate through the rotating shaft 94. The gear 92 drives the reciprocating assembly 9 to reciprocate through the meshing block 93. The reciprocating assembly 9 drives the separation mechanism 15 to reciprocate. The separation mechanism 15 drives the slider mechanism 11 to slide within the slide groove mechanism 10 through the support leg 13. The slider mechanism 11 and the support leg 13 can support the separation mechanism 15 and ensure the stability of the separation mechanism 15. With the reciprocating motion of the separation mechanism 15, the solution inside the separation mechanism 15 shakes, which can accelerate the separation speed.
[0035] It should be noted that the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.
[0036] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A peptide separation device based on membrane separation technology, characterized in that: The polypeptide separation device includes a base support (12), a reciprocating assembly (9) is provided above the base support (12), and a separation mechanism (15) is connected to the top of the reciprocating assembly (9). The separation mechanism (15) is provided with a sealed feeding mechanism (1), a filter screen (2), a first separation system, a second separation system, and an inclined plate (8) from top to bottom. A retaining ring (3) is provided below the filter screen (2), and a cover plate (22) is provided on the top of the separation mechanism (15).
2. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The sealed feeding mechanism (1) includes a feed inlet (23), a sealed cover (24), and a biological deodorizer (25). A trumpet-shaped sealed cover (24) is vertically attached and fixed to the lower surface of the cover plate (22) relative to the left outer side of the separation mechanism (15). The left end of the sealed cover (24) is a small diameter end, and the right end is a large diameter end. The small diameter end is sealed and connected to the feed inlet (23). The biological deodorizer (25) is placed in the middle of the sealed cover (24). The mixed polypeptide liquid enters the sealed cover (24) through the feed inlet (23). After the odor is removed by the biological deodorizer (25), it flows into the filter screen (2) for filtration. A level gauge (21) is also provided on the right inner side wall of the separation mechanism (15). The level gauge (21) is located slightly below the horizontal plane where the large diameter end of the sealed cover (24) is located. The level gauge (21) is used to monitor the amount of mixed polypeptide liquid entering the sealed cover (24) to prevent backflow of the feed liquid.
3. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The filter screen (2) is made of stainless steel.
4. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The first separation system includes a first inlet pipe (20), a primary pump (19), a ceramic membrane (18), a first drain port (17), and a first solenoid valve. The ceramic membrane (18) is vertically spaced below the filter screen (2). The first drain port (17) is also inclined on the right outer side of the separation mechanism (15) relative to the position of the ceramic membrane (18). The left end of the first drain port (17) extends obliquely upward to the plane where the lower edge of the ceramic membrane (18) is located. A first solenoid valve is also provided on the first drain port (17) to control its opening and closing. The pore size of the ceramic membrane (18) is 200nm, which can retain polypeptide molecules with a size of more than 10000Da. The polypeptide molecules that pass through the ceramic membrane (18) are transported to the second separation system by the secondary pump (6), while the polypeptide molecules that cannot pass through the ceramic membrane (18) are collected and discharged through the first drain port (17).
5. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The second separation system includes a second inlet pipe (5), a secondary pump (6), a special separation membrane (7), a second outlet (16), a second solenoid valve, an inclined plate (8), a third outlet (14), and a third solenoid valve; a special separation membrane (7) and an inclined plate (8) are also provided at intervals below the first separation system, the special separation membrane (7) being horizontally arranged within the separation mechanism (15); a second outlet (16) is also provided at an angle on the right outer side of the separation mechanism (15) relative to the position of the special separation membrane (7), the left end of the second outlet (16) extending upwards into the separation mechanism (15), and the upper end of the second outlet (16) near its lower edge being located at the right end of the upper surface of the special separation membrane (7), and a second solenoid valve controlling its opening and closing is also provided on the second outlet (16); the inclined plate (8) The separation mechanism (15) is horizontally inclined downwards to the right side, and a third drain port (14) is inclined on the right outer side of the separation mechanism (15) relative to the inclined plate (8). The left end of the third drain port (14) extends upwards into the separation mechanism (15), and the upper end of the third drain port (14) is located at the lower end of the upper surface of the inclined plate (8) near its lower edge. A third solenoid valve for controlling its opening and closing is also provided on the third drain port (14). The mixed polypeptide liquid through the first separation system is transported to the special separation membrane (7) by the secondary pump (6) for secondary separation. The special separation membrane (7) can retain a molecular weight of 2000 Da, thereby collecting the permeable polypeptide molecules onto the inclined plate (8) and discharging them through the third drain port (14), while the non-permeable polypeptide molecules are discharged through the second drain port (16).
6. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The reciprocating assembly (9) includes an outer frame (91), the bottom of which slides in contact with the top of the base bracket (12), the top of which is fixedly connected to the bottom of the separation mechanism (15), and meshing blocks (93) are fixedly connected to the upper and lower inner walls of the outer frame (91). The meshing blocks (93) are meshed with gears (92), and the gears (92) are connected to the motor through a rotating shaft (94).
7. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The base support (12) is provided with a sliding groove mechanism (10) on both sides of the top. The sliding groove mechanism (10) is provided with a slider mechanism (11). The top of the slider mechanism (11) is fixedly connected to a support leg (13). The top of the support leg (13) is connected to the bottom of the separation mechanism (15).
8. The polypeptide separation device based on membrane separation technology according to claim 1, characterized in that: The separation mechanism (15) has a control mechanism (4) on its left side, which controls the motor, primary pump (19), and secondary pump (6).