High-throughput exosome separation device

Abstract

The utility model belongs to the technical field of exosome separation, and particularly relates to a high-flux exosome separation device which comprises a supporting frame, a limiting frame is fixedly installed on the inner side of the supporting frame, a fixing base is fixedly installed on the inner wall of the bottom of the supporting frame, and a collecting bottle is installed on the outer side of a separation bottle in a threaded mode. At the moment, a separation bottle is inserted into the inner side of a rotating frame through a connecting frame, an operating handle is rotated to drive a second lead screw to rotate, the second lead screw can drive a moving plate and a fixing block to descend through thread fit, the fixing block can slide into the inner side of the connecting frame after descending, and connection of the separation bottle and a collection bottle can be achieved more conveniently and quickly; a limiting rotating shaft linearly moves in a reciprocating manner, a second motor can drive the limiting rotating shaft to rotate, so that a rotating frame is driven to slide and rotate, exosomes on the inner side of a separation bottle are conveniently separated, and separated liquid can enter the inner side of a collection bottle to be collected.

Description

Technical Field

[0001] This utility model relates to the field of exosome separation technology, and in particular to a high-throughput exosome separation device. Background Technology

[0002] Current high-throughput exosome separation devices cannot fully vibrate the stock solution in the separation bottle body through the motor during use, and cannot quickly separate the cells in the stock solution to accelerate the separation and extraction efficiency. Moreover, the current separation bottle body is not very convenient to pick up and put down. To address the above problems, there is a need to provide a high-throughput exosome separation device with good vibration effect and convenient picking up and putting down of the separation bottle.

[0003] CN 219032117 U discloses a high-throughput exosome separation device, including a connecting cavity, a cavity end cap which is rotatably connected to the end face of the connecting cavity via a hinge, and a controller which is connected to the side wall of the connecting cavity.

[0004] In use, the existing device is quickly connected by a spring, but its stability is poor. During shaking, the separation bottle is prone to detaching from the connecting turntable, and the existing device is difficult to control the amplitude of movement. Therefore, we propose a high-throughput exosome separation device. Utility Model Content

[0005] The purpose of this invention is to provide a high-throughput exosome separation device that solves the existing problems.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A high-throughput exosome separation device includes a support frame, a limit frame fixedly installed on the inner side of the support frame, a fixed seat fixedly installed on the bottom inner wall of the support frame, a first motor fixedly installed on the inner side of the fixed seat, a drive shaft fixedly installed on the output end of the first motor, a turntable fixedly installed on one side of the drive shaft, a first lead screw rotatably installed on the inner side of the turntable, a knob fixedly installed on the outer side of one end of the first lead screw, and a moving block threadedly installed on the outer side of the first lead screw.

[0008] As a further improvement to the above solution, a connecting shaft is rotatably mounted on one side of the movable block, a rocker arm is rotatably mounted on the outer side of the connecting shaft, and a connecting lug is rotatably mounted on the outer side of the rocker arm.

[0009] As a further improvement to the above solution, a second motor is fixedly installed on one side of the connecting lug, a limit rotating shaft is fixedly installed at the output end of the second motor, and a rotating frame is fixedly installed on the top of the limit rotating shaft.

[0010] As a further improvement to the above solution, multiple connecting frames are provided on the outer side of the rotating frame, a separation bottle is fixedly installed on the inner side of the connecting frame, and a collection bottle is threadedly installed on the outer side of the separation bottle.

[0011] As a further improvement to the above solution, a plurality of second lead screws are rotatably mounted on the inner side of the rotating frame, an operating handle is fixedly mounted on the top of the second lead screws, a moving plate is threadedly mounted on the outer side of the second lead screws, and a fixing block is fixedly mounted on the bottom of the moving plate.

[0012] As a further improvement to the above solution, multiple mounting slots are provided around the outer side of the rotating frame, and the connecting frame fits into the mounting slots.

[0013] As a further improvement to the above solution, the top of the connecting frame is provided with a fixing groove, the inner side of the rotating frame is provided with multiple limiting grooves, the moving plate is slidably installed on the inner side of the limiting groove, the fixing block is in contact with the fixing groove, the outer side of the separating bottle is provided with external threads, and the inner side of the collecting bottle is provided with internal threads.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] (1) A high-throughput exosome separation device of the present invention is provided, in which the collection bottle is threadedly installed on the outside of the separation bottle, and the separation bottle is inserted into the inside of the rotating frame through the connecting frame. The operation handle is rotated to drive the second lead screw to rotate. The second lead screw can drive the moving plate and the fixed block to descend through the threaded engagement. After the fixed block descends, it can slide into the inside of the connecting frame, which can realize the connection between the separation bottle and the collection bottle in a more convenient and quick way.

[0016] (2) A high-throughput exosome separation device of the present invention has a limiting rotating shaft that moves back and forth in a linear motion. The second motor can drive the limiting rotating shaft to rotate, thereby driving the rotating frame to slide and rotate, so as to facilitate the separation of exosomes inside the separation bottle. The separated liquid can enter the inside of the collection bottle for collection. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a three-dimensional structural diagram of a high-throughput exosome separation device proposed in this utility model;

[0019] Figure 2 This is a three-dimensional structural diagram of the explosive portion of a high-throughput exosome separation device proposed in this utility model;

[0020] Figure 3 for Figure 2 A schematic diagram of the structure of part A in the diagram;

[0021] Figure 4 This is a cross-sectional three-dimensional structural diagram of a high-throughput exosome separation device proposed in this utility model.

[0022] In the diagram: 1. Support frame; 2. Limiting frame; 3. Fixed seat; 4. First motor; 5. Drive shaft; 6. Turntable; 7. First lead screw; 8. Knob; 9. Moving block; 10. Connecting shaft; 11. Rocker arm; 12. Connecting lug; 13. Second motor; 14. Limiting rotation shaft; 15. Rotating frame; 16. Connecting frame; 17. Separating bottle; 18. Collecting bottle; 19. Second lead screw; 20. Operating handle; 21. Moving plate; 22. Fixed block. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0024] refer to Figure 1-4A high-throughput exosome separation device includes a support frame 1, a limit frame 2 fixedly installed on the inner side of the support frame 1, a fixed seat 3 fixedly installed on the bottom inner wall of the support frame 1, a first motor 4 fixedly installed on the inner side of the fixed seat 3, a drive shaft 5 fixedly installed on the output end of the first motor 4, a turntable 6 fixedly installed on one side of the drive shaft 5, a first lead screw 7 rotatably installed on the inner side of the turntable 6, a knob 8 fixedly installed on the outer side of one end of the first lead screw 7, and a moving block 9 threadedly installed on the outer side of the first lead screw 7; the knob 8 can be controlled to drive the first lead screw 7 to rotate according to the amplitude required for exosome separation, and the first lead screw 7 can drive the moving block 9 to move its position inside the turntable 6, which can change the distance between the connecting shaft 10 and the center of the turntable 6; in this embodiment, a connecting shaft 10 is rotatably installed on one side of the moving block 9. A connecting shaft 10 is connected to a rocker arm 11, which is rotatably mounted on its outer side. A connecting lug 12 is rotatably mounted on the outer side of the rocker arm 11. The first motor 4 drives the drive shaft 5 and the turntable 6 to rotate. The turntable 6 can drive the connecting shaft 10, rocker arm 11, connecting lug 12, second motor 13, and limiting rotating shaft 14 to reciprocate linearly. The second motor 13 can drive the limiting rotating shaft 14 to rotate, thereby driving the rotating frame 15 to slide and rotate, which facilitates the separation of exosomes inside the separation bottle 17. The separated liquid can enter the inside of the collection bottle 18 for collection. In this embodiment, the second motor 13 is fixedly mounted on one side of the connecting lug 12. The limiting rotating shaft 14 is fixedly mounted on the output end of the second motor 13. The rotating frame 15 is fixedly mounted on the top of the limiting rotating shaft 14.

[0025] In this embodiment, multiple connecting frames 16 are provided on the outer side of the rotating frame 15. A separation bottle 17 is fixedly installed on the inner side of the connecting frame 16, and a collection bottle 18 is threadedly installed on the outer side of the separation bottle 17. The collection bottle 18 is threadedly installed on the outer side of the separation bottle 17. At this time, the separation bottle 17 is inserted into the inner side of the rotating frame 15 through the connecting frame 16. Rotating the operating handle 20 drives the second lead screw 19 to rotate. The second lead screw 19 can drive the moving plate 21 and the fixing block 22 to descend through the threaded engagement. After descending, the fixing block 22 can slide into the inner side of the connecting frame 16, which can realize the connection of the separation bottle 17 and the collection bottle 18 in a convenient and quick way. In this embodiment, multiple second lead screws 19 are rotatably installed on the inner side of the rotating frame 15. An operating handle 20 is fixedly installed on the top of the second lead screw 19, a moving plate 21 is threadedly installed on the outer side of the second lead screw 19, and a fixing block 22 is fixedly installed on the bottom of the moving plate 21.

[0026] In this embodiment, the outer side of the rotating frame 15 is provided with multiple mounting slots, and the connecting frame 16 fits into the mounting slots. The mounting slots facilitate the insertion of the connecting frame 16 into the inner side of the rotating frame 15, and the fixing block 22 locks the connecting frame 16 through the cooperation of the fixing block 22 with the fixing slot, so that the connecting frame 16, the separation bottle 17 and the collection bottle 18 are not prone to detachment during the lifting and rotation of the rotating frame 15. In this embodiment, the top of the connecting frame 16 is provided with a fixing slot, the inner side of the rotating frame 15 is provided with multiple limiting slide grooves, the moving plate 21 is slidably installed on the inner side of the limiting slide grooves, the fixing block 22 fits into the fixing slots, the outer side of the separation bottle 17 is provided with external threads, and the inner side of the collection bottle 18 is provided with internal threads.

[0027] The implementation principle of a high-throughput exosome separation device in this application embodiment is as follows: A collection bottle 18 is threaded onto the outside of a separation bottle 17. The separation bottle 17 is then inserted into the inside of a rotating frame 15 via a connecting bracket 16. Rotating the operating handle 20 drives the second lead screw 19 to rotate. The second lead screw 19, through threaded engagement, drives the moving plate 21 and the fixing block 22 to descend. After descending, the fixing block 22 slides into the inside of the connecting bracket 16, making the connection between the separation bottle 17 and the collection bottle 18 convenient and quick. The knob 8 can be controlled to drive the first lead screw 7 according to the required amplitude for exosome separation. Rotation causes the first lead screw 7 to move the moving block 9 within the turntable 6, changing the distance between the connecting shaft 10 and the center of the turntable 6. This controls the first motor 4 to drive the drive shaft 5 and the turntable 6 to rotate. The turntable 6 can then drive the connecting shaft 10, rocker arm 11, connecting lug 12, second motor 13, and limiting rotating shaft 14 to reciprocate linearly. The second motor 13 can drive the limiting rotating shaft 14 to rotate, thereby enabling the rotating frame 15 to slide and rotate, facilitating the separation of exosomes inside the separation bottle 17. The separated liquid can then enter the inside of the collection bottle 18 for collection.

[0028] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0029] The high-throughput exosome separation device provided by this utility model has been described in detail above. Specific embodiments have been used to illustrate the principle and implementation of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core idea of ​​this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made to this utility model without departing from the principle of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. A high-throughput exosome separation device, characterized in that, include: A support frame (1) is fixedly installed on the inner side of the support frame (1). A fixed seat (3) is fixedly installed on the bottom inner wall of the support frame (1). A first motor (4) is fixedly installed on the inner side of the fixed seat (3). A drive shaft (5) is fixedly installed on the output end of the first motor (4). A turntable (6) is fixedly installed on one side of the drive shaft (5). A first lead screw (7) is rotatably installed on the inner side of the turntable (6). A knob (8) is fixedly installed on the outer side of one end of the first lead screw (7). A moving block (9) is threaded on the outer side of the first lead screw (7).

2. The high-throughput exosome separation device according to claim 1, characterized in that, A connecting shaft (10) is rotatably mounted on one side of the movable block (9), a rocker arm (11) is rotatably mounted on the outside of the connecting shaft (10), and a connecting lug (12) is rotatably mounted on the outside of the rocker arm (11).

3. The high-throughput exosome separation device according to claim 2, characterized in that, A second motor (13) is fixedly installed on one side of the connecting lug (12), and a limit rotating shaft (14) is fixedly installed at the output end of the second motor (13). A rotating frame (15) is fixedly installed on the top of the limit rotating shaft (14).

4. The high-throughput exosome separation device according to claim 3, characterized in that, The rotating frame (15) is provided with multiple connecting frames (16) on its outer side. A separation bottle (17) is fixedly installed on the inner side of the connecting frame (16), and a collection bottle (18) is threadedly installed on the outer side of the separation bottle (17).

5. A high-throughput exosome separation device according to claim 4, characterized in that, Multiple second lead screws (19) are rotatably mounted on the inner side of the rotating frame (15). An operating handle (20) is fixedly mounted on the top of the second lead screw (19). A moving plate (21) is threadedly mounted on the outer side of the second lead screw (19). A fixing block (22) is fixedly mounted on the bottom of the moving plate (21).

6. The high-throughput exosome separation device according to claim 3, characterized in that, The rotating frame (15) has multiple mounting slots around its outer circumference, and the connecting frame (16) fits into the mounting slots.

7. The high-throughput exosome separation device according to claim 4, characterized in that, The top of the connecting frame (16) is provided with a fixing groove, the inner side of the rotating frame (15) is provided with multiple limiting grooves, the moving plate (21) is slidably installed on the inner side of the limiting groove, the fixing block (22) is in contact with the fixing groove, the outer side of the separating bottle (17) is provided with an external thread, and the inner side of the collecting bottle (18) is provided with an internal thread.

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