MTHFR gene detection kit capable of avoiding leakage

Abstract

The utility model discloses an MTHFR gene detection kit capable of avoiding leakage, and relates to the field of detection kits.The MTHFR gene detection kit comprises a kit body, the two ends of the top of the kit body are rotationally connected with kit covers through hinges, a placement plate is fixed to an inner cavity of the kit body, placement grooves are formed in the placement plate at equal intervals, and placement vessels are arranged in the placement grooves; a protection mechanism for sealing the placing vessel is arranged at the top of the inner cavity of the box body; and an auxiliary mechanism for assisting in taking out the placing vessel is arranged at the bottom of the placing vessel. Through the arrangement of the protection mechanism, the storage environment of the inner cavity of the box body can be adjusted under the action of the refrigeration box in the use process, so that samples can be ensured to be in a low-temperature storage environment, and meanwhile, the interior of the placing vessel can be sealed from the top of the placing vessel under the action of a sealing head; therefore, in the process of moving the box body, the sample is prevented from moving out of the interior of the placing vessel.

Description

Technical Field

[0001] This invention relates to the field of detection kits, specifically to an MTHFR gene detection kit that prevents leakage. Background Technology

[0002] The 5,10-methylenetetrahydrofolate reductase (MTHFR) gene encodes methylenetetrahydrofolate reductase, a key enzyme in folate metabolism. Folate deficiency is a risk factor for birth defects and cardiovascular and cerebrovascular diseases caused by hyperhomocysteinemia. The frequencies of the MTHFR gene C677T polymorphism in the Chinese population are approximately 27% for the CC type, 49% for the CT type, and 24% for the TT type. Taking the wild-type CC type MTHFR enzyme activity as 100%, the heterozygous CT type has an MTHFR enzyme activity of only about 60%, and the mutant TT type has an activity reduced to 30%. Folate metabolism disorders caused by reduced enzyme activity can be corrected by folate supplementation. Therefore, detecting the genotype of the MTHFR gene C677T polymorphism is particularly important for the prevention and treatment of birth defects and cardiovascular and cerebrovascular diseases, and has gradually become a routine clinical test for related diseases.

[0003] An existing application (application number 202223533154.6) discloses a real-time quantitative PCR detection kit for human MTHFR gene, comprising a box body and a lid. The lid is located at the top of the box body and includes a first lid and a second lid. Sliding grooves are provided on both the front and rear sides of the box body. Limiting plates are installed on both the left and right sides of the front and rear sides of the box body. Connecting plates are provided on the right side of the lower end of the first lid and the left side of the lower end of the second lid. An extension plate is installed at the lower end of the connecting plate. A magnetic strip is fixedly installed on the side of the extension plate near the limiting plate. A metal plate is fixedly installed on the side of the limiting plate near the extension plate. The metal plate is made of a metal that can be magnetically attracted. By adding limiting and magnetic structures to the lid, a limiting and fixing function is achieved to prevent the lid from automatically returning to its original position.

[0004] In order to prevent the test samples inside the placement dish from tipping over or being removed from the kit during use, the existing test kits usually have a tight connection between the placement dish and the placement plate. However, this makes it difficult to remove the placement dish from the placement plate during testing.

[0005] Therefore, in view of this, we have studied and improved the existing structure and deficiencies, and proposed a leakage-avoiding MTHFR gene detection kit. Utility Model Content

[0006] The purpose of this invention is to provide an MTHFR gene detection kit that avoids leakage, so as to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a leakage-avoiding MTHFR gene detection kit, comprising a box body, wherein both ends of the top of the box body are rotatably connected to a box lid via hinges, a placement plate is fixed in the inner cavity of the box body, and placement slots are equidistantly provided inside the placement plate, and a placement dish is provided inside the placement slots.

[0008] The top of the inner cavity of the box is provided with a protective mechanism for sealing the container.

[0009] The bottom of the placement dish is provided with an auxiliary mechanism to help remove the placement dish.

[0010] Furthermore, the protective mechanism includes a sealing plate fixed to the top center of the lid, and a storage box at the bottom of the sealing plate, inside which a cooling box is placed. The cooling box lowers the temperature inside the box to ensure the quality of the sample placed in the dish.

[0011] Furthermore, a sealing groove is provided inside the center of the top of the box body, and the outer surface of the storage box is in contact with the inner surface of the inner cavity of the box body. The storage box and the box body are movably connected, and sealing heads are evenly distributed at the bottom of the storage box. The sealing heads can isolate the sample in the placement dish from the top of the placement dish, preventing sample leakage.

[0012] Furthermore, the auxiliary mechanism includes a lifting groove located at the bottom of the placement groove. A lifting plate is slidably connected inside the lifting groove, and a push spring is fixed at the bottom center of the lifting plate. The outer surface of the lifting plate is in contact with the inner surface of the placement groove. Through the action of the push spring and the lifting plate, the placement dish can be partially moved out of the placement plate, facilitating its removal.

[0013] Furthermore, an insertion slot is provided at the center of the lifting plate, and an insertion pin is slidably connected inside the insertion slot. A traction buckle is fixed to the side of the insertion pin away from the center of the box. The insertion pin can be used to fix the position of the placement dish inside the placement plate.

[0014] Furthermore, limiting pins are fixed on both sides of the top of the placement dish located at the center. These limiting pins are embedded inside the placement plate, and each limiting pin has a limiting buckle at its top. The end of the limiting buckle away from the limiting pin is rotatably connected to the placement plate. The limiting pins and the limiting buckle effectively fix the position of the placement dish embedded inside the placement plate.

[0015] Furthermore, handles are fixed at both ends of the top of the lid near the center, and the dish is movably connected to the placement plate via limiting pins and limiting buckles. By rotating the limiting buckles, the dish located at the center can be moved out of the placement plate.

[0016] This invention provides an MTHFR gene detection kit that avoids leakage, and has the following beneficial effects:

[0017] 1. Through the setting of the protective mechanism, this utility model can adjust the storage environment of the inner cavity of the box during use by means of the cooling box, thereby ensuring that the sample can be stored in a low temperature environment. At the same time, through the function of the sealing head, the inside of the placement dish can be sealed from the top of the placement dish, thereby preventing the sample from being moved out of the placement dish during the movement of the box.

[0018] 2. By setting up an auxiliary mechanism, when it is necessary to remove the placement dish and test the sample, the insertion pin corresponding to the position of the placement dish is removed. Then, by the action of the push spring, the lifting plate and the placement dish are partially raised inside the placement plate, so that the top of the placement dish is partially moved out of the placement plate. This setting makes it convenient to remove the placement dish. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the MTHFR gene detection kit that prevents leakage according to the present invention.

[0020] Figure 2 This is a schematic cross-sectional view of the box structure of the MTHFR gene detection kit for preventing leakage according to this utility model.

[0021] Figure 3 This is a partial top view of the box structure of the MTHFR gene detection kit for preventing leakage according to this utility model.

[0022] Figure 4 This is a partial cross-sectional view of the box structure of the MTHFR gene detection kit that prevents leakage according to this utility model.

[0023] In the diagram: 1. Box body; 2. Hinge; 3. Box lid; 4. Placement plate; 5. Placement dish; 6. Sealing plate; 7. Storage box; 8. Cooling box; 9. Lifting plate; 10. Push spring; 11. Insertion pin; 12. Traction buckle; 13. Limit pin; 14. Limit buckle; 15. Sealing head; 16. Handle. Detailed Implementation

[0024] The following will be combined with the appendix Figures 1 to 4This utility model will be described in detail, and the technical solutions in the embodiments of this utility model will be clearly and completely described. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0025] This utility model provides an improved fixing clamp for steel pipe perforation processing, such as... Figures 1-4 As shown, the box includes a box body 1. Both ends of the top of the box body 1 are rotatably connected to a box cover 3 via hinges 2. A placement plate 4 is fixed inside the box body 1. Placement slots are equidistantly opened inside the placement plate 4. Placement dishes 5 are placed inside the placement slots. A protective mechanism for sealing the placement dishes 5 is provided at the top of the inner cavity of the box body 1. An auxiliary mechanism for assisting in the removal of the placement dishes 5 is provided at the bottom of the placement dishes 5.

[0026] In this embodiment: the test sample is placed inside the placement dish 5, and then the placement dish 5 is placed in the placement slot inside the placement plate 4. Since both ends of the top of the box body 1 are rotatably connected to the box cover 3 by the hinge 2, the box cover 3 can be partially rotated around the center of the hinge 2 by the action of the handle 16, thereby opening the box cover 3.

[0027] In the first implementation, because the top of the placement dish 5 is open, sample leakage is likely to occur. The specific operation is as follows:

[0028] like Figure 2 and Figure 4 As shown, the protective mechanism includes a sealing plate 6, which is fixed to the top center of the box cover 3. A storage box 7 is provided at the bottom of the sealing plate 6, and a cooling box 8 is placed inside the storage box 7. A sealing groove is opened inside the top center of the box body 1. The outer surface of the storage box 7 is in contact with the inner surface of the inner cavity of the box body 1. The storage box 7 and the box body 1 are movably connected. Sealing heads 15 are evenly distributed at the bottom of the storage box 7.

[0029] In this embodiment: the storage box 7 is placed inside the box body 1 and installed through the sealing groove. Then, the cooling box 8 is placed inside the storage box 7. When the box cover 3 is closed, the box body 1 can be sealed by the sealing plate 6 to prevent the external environment from affecting the sample. At the same time, through the function of the sealing head 15, when the box body 1 is completely sealed, the placement dish 5 is sealed by the sealing head 15 to prevent sample leakage.

[0030] In the previous implementation scheme, because the connection between the placement dish 5 and the placement plate 4 is relatively tight, it is difficult to remove the placement dish 5. The specific operation is as follows:

[0031] like Figure 2 and Figure 3 as well as Figure 4 As shown, the auxiliary mechanism includes a lifting groove located at the bottom of the placement groove. A lifting plate 9 is slidably connected inside the lifting groove, and a push spring 10 is fixed at the center of the bottom of the lifting plate 9. The outer surface of the lifting plate 9 is in contact with the inner surface of the placement groove. An insertion groove is provided at the center of the lifting plate 9, and an insertion pin 11 is slidably connected inside the insertion groove. A traction buckle 12 is fixed on the side of the insertion pin 11 away from the center of the box body 1. Limiting pins 13 are fixed on both sides of the top of the placement dish 5 located at the center. The limiting pins 13 are embedded in the interior of the placement plate 4, and a limiting buckle 14 is provided on the top of the limiting pin 13. The end of the limiting buckle 14 away from the limiting pin 13 is rotatably connected to the placement plate 4. Handles 16 are fixed at both ends of the top of the box lid 3 near the center. The placement dish 5 is movably connected to the placement plate 4 through the limiting pins 13 and the limiting buckles 14.

[0032] In this embodiment: After opening the box cover 3, the storage box 7 is taken out. According to the position of the corresponding placement dish 5 to be taken out, the corresponding insertion pin 11 is taken out by the traction buckle 12. Then, by the action of the push spring 10, the position of the lifting plate 9 and the placement dish 5 is partially raised, so that the top of the placement dish 5 can be partially moved out, thereby facilitating the removal of the placement dish 5. The placement dish 5 at the center position is fixed in position by the action of the limiting pin 13 and the limiting buckle 14. When it is necessary to remove the placement dish 5 located at the center, the limiting buckle 14 is rotated, and by the action of the push spring 10, the lifting plate 9 and the placement dish 5 are pushed up, thereby facilitating the removal of the placement dish 5 located at the center.

[0033] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A leakage-avoiding MTHFR gene detection kit, characterized in that, Includes a box body (1), both ends of the top of the box body (1) are rotatably connected to a box lid (3) via hinges (2), a placement plate (4) is fixed inside the box body (1), and a placement slot is provided at equal intervals inside the placement plate (4), and a placement dish (5) is provided inside the placement slot; The top of the inner cavity of the box (1) is provided with a protective mechanism for sealing the container (5); The bottom of the placement dish (5) is provided with an auxiliary mechanism for assisting in the removal of the placement dish (5).

2. The MTHFR gene detection kit for preventing leakage according to claim 1, characterized in that, The protective mechanism includes a sealing plate (6), which is fixed at the top center of the box cover (3). A storage box (7) is provided at the bottom of the sealing plate (6), and a refrigeration box (8) is placed inside the storage box (7).

3. The MTHFR gene detection kit for preventing leakage according to claim 2, characterized in that, A sealing groove is provided inside the top center of the box body (1), the outer surface of the storage box (7) is in contact with the inner surface of the inner cavity of the box body (1), the storage box (7) and the box body (1) are movably connected, and sealing heads (15) are distributed at equal intervals at the bottom of the storage box (7).

4. The MTHFR gene detection kit for preventing leakage according to claim 1, characterized in that, The auxiliary mechanism includes a lifting groove, which is opened at the bottom of the placement groove. A lifting plate (9) is slidably connected inside the lifting groove. A push spring (10) is fixed at the bottom center of the lifting plate (9). The outer surface of the lifting plate (9) is in contact with the inner surface of the placement groove.

5. The MTHFR gene detection kit for preventing leakage according to claim 4, characterized in that, An insertion slot is provided at the center of the lifting plate (9), and an insertion pin (11) is slidably connected inside the insertion slot. A traction buckle (12) is fixed on the side of the insertion pin (11) away from the center of the box (1).

6. The MTHFR gene detection kit for preventing leakage according to claim 1, characterized in that, Limiting pins (13) are fixed on both sides of the top of the placement dish (5) located at the center. The limiting pins (13) are embedded in the interior of the placement plate (4). The top of the limiting pins (13) is provided with a limiting buckle (14). The end of the limiting buckle (14) away from the limiting pins (13) is rotatably connected to the placement plate (4).

7. The MTHFR gene detection kit for preventing leakage according to claim 1, characterized in that, The top of the lid (3) is fixed with handles (16) at both ends near the center. The placement dish (5) is movably connected to the placement plate (4) through a limiting pin (13) and a limiting buckle (14).

Images