Device for extracting and decoloring hirudin

Abstract

The utility model discloses a device for extracting and decoloring hirudin, and relates to the technical field of extraction of hirudin. Comprising a support, a motor controller is installed on one side of the support, an electric push rod is installed at the top of the support and electrically connected with the motor controller through a wire, a column tube is installed in the middle of the support, a liquid outlet head is arranged at the bottom of the column tube, and the column tube is sequentially filled with absorbent cotton and hydrophobic chromatographic filler from bottom to top. A rubber piston is slidably mounted on the upper portion of the column pipe. According to the device for extracting and decolorizing the hirudin, the surface of the adopted hydrophobic chromatographic filler is usually covered with hydrophobic chains or hydrophobic groups, so that the hydrophobic chromatographic filler has a hydrophilic effect on polar substances, the hydrophobic chromatographic filler can effectively separate hydrophobic substances due to high hydrophobicity, and the separation effect of the hydrophobic chromatographic filler is superior to that of other fillers; the electric push rod drives the rubber piston to press down, so that the flow rate of the target solution is controlled as required, the separation time is shortened, and the decolorization efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of hirudin extraction technology, specifically to a device for hirudin extraction and decolorization. Background Technology

[0002] Hirudin is a polypeptide extracted and isolated from the salivary glands of medicinal leeches. It is the most potent thrombin discovered to date, exhibiting excellent anticoagulant and antithrombotic effects. It has good preventive and therapeutic effects on various thrombotic diseases such as venous thrombosis, disseminated intravascular coagulation, cerebral thrombosis, thrombophlebitis, and coronary thrombosis. Due to the limited production of natural hirudin, the extraction yield from the head of a leech is only 2 μg / leech, which cannot meet the requirements of clinical applications. Therefore, the domestic and international pharmaceutical communities have focused on researching the acquisition of recombinant hirudin through genetic engineering. Based on the known amino acid sequence of hirudin, the corresponding sequence DNA fragment is synthesized in vitro, inserted into a recombinant expression vector, and expressed in Escherichia coli and yeast cells. The Pichia pastoris expression system is a high-expression eukaryotic system with secretory advantage, exhibiting high secretion expression levels, good stability of the engineered yeast, and the ability to significantly improve the expression level of the product under high-density fermentation conditions.

[0003] After the successful construction and expression of recombinant hirudin, it is necessary to separate and purify the recombinant hirudin. Optimized separation and purification methods can improve the purity of hirudin and reduce costs. Traditional methods for separating recombinant hirudin to remove pigment molecules usually adopt a two-step approach: 1. Concentrate hirudin using ultrafiltration membrane; 2. Precipitate the concentrated hirudin with low-temperature ethanol precipitation to separate the pigment molecules.

[0004] Existing separation technologies suffer from problems because the fermentation process produces protein-degrading enzymes that hydrolyze hirudin activity. As a result, the ultrafiltration concentration time is too long, which is not conducive to the stability of hirudin activity. Furthermore, the consumption of ultrafiltration membranes is high, resulting in high concentration costs. The two-step method also consumes a large amount of ethanol, which is not environmentally friendly. Utility Model Content

[0005] This invention provides a device for extracting and decolorizing hirudin to solve the problems in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a device for extracting and decolorizing hirudin, comprising a support, a motor controller mounted on one side of the support, an electric push rod mounted on the top of the support, and the electric push rod electrically connected to the motor controller via a wire, a column tube mounted in the middle of the support, and a liquid outlet head provided at the bottom of the column tube, the column tube being filled with degreased cotton and hydrophobic chromatographic packing material from bottom to top, a rubber piston slidably mounted on the upper part of the column tube, and a piston rod connected to the top of the rubber piston, a pressure plate connected to the output end of the electric push rod, and the pressure plate pressing against the piston rod, and a collection cup placed at the bottom of the support.

[0007] Furthermore, the bracket has a sleeve hole in the middle, through which the column tube is snapped into the middle of the bracket.

[0008] Furthermore, the lower part of the column tube is provided with a washer ring, and the washer ring is located above the sleeve hole.

[0009] Furthermore, the top two ends of the bracket are provided with guide holes, and the two ends of the pressure plate are inlaid with guide rods, which are movably connected with the guide holes.

[0010] Furthermore, the pressure plate is located above the piston rod, and the collection cup is located below the liquid outlet head.

[0011] Furthermore, the inner bottom of the bracket is provided with a positioning groove, and the collecting cup is placed in the positioning groove.

[0012] Compared with the prior art, this utility model provides a device for extracting and decolorizing hirudin, which has the following beneficial effects:

[0013] The device for extracting and decolorizing hirudin uses hydrophobic chromatographic packing material whose surface is typically covered with hydrophobic chains or hydrophobic groups, making it hydrophilic to polar substances. Due to its strong hydrophobicity, the hydrophobic chromatographic packing material can effectively separate hydrophobic substances, and its separation effect is better than other packing materials. The flow rate of the target solution is controlled by an electric push rod that drives the rubber piston to press down, thereby shortening the separation time and improving the decolorization efficiency. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a cross-sectional view of the present invention;

[0016] Figure 3 This is a side view of the present invention.

[0017] In the diagram: 1. Support; 2. Motor controller; 3. Electric push rod; 4. Column tube; 5. Liquid outlet head; 6. Degreased cotton; 7. Hydrophobic chromatography packing material; 8. Rubber piston; 9. Piston rod; 10. Pressure plate; 11. Collection cup; 12. Sleeve hole; 13. Washer ring; 14. Guide hole; 15. Guide rod; 16. Positioning groove. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1-3 This utility model discloses a device for extracting and decolorizing hirudin, including a support 1. A motor controller 2 is installed on one side of the support 1, and an electric push rod 3 is installed on the top of the support 1. The electric push rod 3 is electrically connected to the motor controller 2 via a wire. A column tube 4 is installed in the middle of the support 1, and a liquid outlet head 5 is provided at the bottom of the column tube 4. The column tube 4 is filled with degreased cotton 6 and hydrophobic chromatographic packing material 7 from bottom to top. A rubber piston 8 is slidably installed on the upper part of the column tube 4, and a piston rod 9 is connected to the top of the rubber piston 8. The output end of the electric push rod 3 is connected to a pressure plate 10, which presses against the piston rod 9. The surface of the hydrophobic chromatographic packing 7 is usually covered with hydrophobic chains or hydrophobic groups, which makes it hydrophilic to polar substances. Due to its strong hydrophobicity, the hydrophobic chromatographic packing 7 can effectively separate hydrophobic substances, and its separation effect is better than other packings. The electric push rod 3 drives the rubber piston 8 to press down, thereby controlling the flow rate of the target solution according to the requirements, shortening the separation time and improving the decolorization efficiency. A collection cup 11 is placed at the lower part of the support 1.

[0020] Specifically, the bracket 1 has a sleeve hole 12 in the middle, and the column tube 4 is snapped into the middle of the bracket 1 through the sleeve hole 12.

[0021] In this embodiment, the sleeve hole 12 is an assembly structure used for the installation and fixing of the column tube 4.

[0022] Specifically, the lower part of the column tube 4 is provided with a washer 13, and the washer 13 is located above the sleeve hole 12.

[0023] In this embodiment, the washer ring 13 is a support structure that serves as a limit after the column tube 4 is tightly fitted into the sleeve hole 12.

[0024] Specifically, the top two ends of the bracket 1 are provided with guide holes 14, and the two ends of the pressure plate 10 are inlaid with guide rods 15, and the guide rods 15 are movably connected with the guide holes 14.

[0025] In this embodiment, the cooperation between the guide rod 15 and the guide hole 14 makes the pressure plate 10 move up and down more smoothly.

[0026] Specifically, the pressure plate 10 is located above the piston rod 9, and the collection cup 11 is located below the liquid outlet head 5.

[0027] In this embodiment, the electric push rod 3 is controlled by the motor controller 2 to press down. The electric push rod 3 drives the pressure plate 10 to descend and press against the piston rod 9, thereby driving the rubber piston 8 to descend, thereby controlling the flow rate of the target solution (based on the principle of pressure difference, the faster the descent, the faster the flow rate, and vice versa).

[0028] Specifically, the inner bottom of the bracket 1 is provided with a positioning groove 16, and the collecting cup 11 is placed in the positioning groove 16.

[0029] In this embodiment, the positioning groove 16 is used to limit the position of the collection cup 11 after it has been placed.

[0030] In use, the surface of the hydrophobic chromatographic packing 7 is usually covered with hydrophobic chains or hydrophobic groups, making it hydrophilic to polar substances. Due to its strong hydrophobicity, the hydrophobic chromatographic packing 7 can effectively separate hydrophobic substances, and its separation effect is better than other packings. The electric push rod 3 is controlled by the motor controller 2 to press down the electric push rod 3. The electric push rod 3 drives the pressure plate 10 to descend and press against the piston rod 9, thereby driving the rubber piston 8 to descend. Based on the principle of pressure difference, the flow rate is high when the descent is fast, and vice versa. Thus, the flow rate of the target solution can be controlled according to the requirements, which can shorten the separation time and improve the decolorization efficiency.

[0031] In summary, the device for extracting and decolorizing hirudin uses a hydrophobic chromatographic packing 7 whose surface is typically covered with hydrophobic chains or hydrophobic groups, making it hydrophilic to polar substances. Due to its strong hydrophobicity, the hydrophobic chromatographic packing 7 can effectively separate hydrophobic substances, and its separation effect is better than other packings. The electric push rod 3 drives the rubber piston 8 to press down, thereby controlling the flow rate of the target solution according to the requirements, which shortens the separation time and improves the decolorization efficiency.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for extracting and decolorizing hirudin, comprising a support (1), characterized in that: A motor controller (2) is installed on one side of the bracket (1), an electric push rod (3) is installed on the top of the bracket (1), and the electric push rod (3) is electrically connected to the motor controller (2) through a wire. A column tube (4) is installed in the middle of the bracket (1), and a liquid outlet head (5) is provided at the bottom of the column tube (4). The column tube (4) is filled with degreased cotton (6) and hydrophobic chromatographic packing material (7) from bottom to top. A rubber piston (8) is slidably installed on the upper part of the column tube (4), and a piston rod (9) is connected to the top of the rubber piston (8). A pressure plate (10) is connected to the output end of the electric push rod (3), and the pressure plate (10) presses against the piston rod (9). A collection cup (11) is placed at the bottom of the bracket (1).

2. The apparatus for extracting and decolorizing hirudin according to claim 1, characterized in that: The bracket (1) has a sleeve hole (12) in the middle, and the column tube (4) is snapped into the middle of the bracket (1) through the sleeve hole (12).

3. The apparatus for extracting and decolorizing hirudin according to claim 2, characterized in that: The lower part of the column tube (4) is provided with a gasket (13), and the gasket (13) is located above the sleeve hole (12).

4. The apparatus for extracting and decolorizing hirudin according to claim 1, characterized in that: The top two ends of the bracket (1) are provided with guide holes (14), and the two ends of the pressure plate (10) are inlaid with guide rods (15), and the guide rods (15) are movably connected with the guide holes (14).

5. The apparatus for extracting and decolorizing hirudin according to claim 1, characterized in that: The pressure plate (10) is located above the piston rod (9), and the collection cup (11) is located below the liquid outlet (5).

6. The apparatus for extracting and decolorizing hirudin according to claim 1, characterized in that: The bracket (1) has a positioning groove (16) at its inner bottom, and the collection cup (11) is placed in the positioning groove (16).

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