Continuous flow microreactor for heparinase catalytic reaction
By designing a continuous flow microreactor for heparinase catalysis, rapid replacement and sealing of immobilized heparinase were achieved, solving the problem of long downtime in microreactors and improving the efficiency of heparinase catalysis.
Patent Information
- Application Number
- CN202423311321.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2034-12-31
AI Technical Summary
In heparinase catalytic reactions, when the activity of immobilized heparinase decreases or its structure changes, the microreactor needs to be disassembled to replace the immobilized heparinase, resulting in long downtime and affecting the efficiency of the catalytic reaction.
A continuous flow microreactor for heparinase catalysis was designed. Through components such as a limiting support base, a positioning mounting frame, and the reactor body, the first and second storage tanks can be quickly replaced, avoiding disassembly of the microreactor. A rubber sealing ring is used for sealing to ensure the normal operation of the reactor.
This improved the replacement efficiency of immobilized heparinase, reduced the downtime of the microreactor, increased the efficiency of the heparinase catalytic reaction, prevented leakage, and ensured the normal operation of the reactor.
Smart Images
Figure CN223837404U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of microreactor technology, and more specifically, relates to a continuous flow microreactor for heparinase-catalyzed reactions. Background Technology
[0002] In the process of heparinase catalysis, immobilized heparinase is first filled into a microreactor to form a stable column bed. Then, the substrate solution is passed through the immobilized heparinase column bed in a certain direction. Under the catalysis of the enzyme, the product is generated, and the conversion liquid containing the product is continuously collected and output. At the same time, the microreactor can use continuous flow technology to precisely control parameters such as the flow rate, residence time and reaction temperature of the reactants. It has high-efficiency mass and heat transfer performance, which can realize the rapid and stable heparinase catalysis reaction.
[0003] Based on the above, when the activity of the immobilized heparinase decreases or its structure changes, the microreactor needs to be disassembled to replace the immobilized heparinase. This process takes a long time, resulting in a long downtime of the microreactor and affecting the efficiency of the heparinase catalytic reaction. Utility Model Content
[0004] To address the aforementioned technical problems, this invention provides a continuous flow microreactor for heparinase catalysis, which solves the problem that when the activity of immobilized heparinase decreases or its structure changes, the microreactor needs to be disassembled to replace the immobilized heparinase. This process takes a long time, resulting in prolonged downtime of the microreactor and affecting the efficiency of the heparinase catalysis.
[0005] The purpose and effectiveness of this novel continuous flow microreactor for heparinase catalysis are achieved through the following specific technical means:
[0006] A continuous flow microreactor for heparinase catalysis includes a limiting support base, a positioning mounting frame, a reactor body, a positioning connecting rod, a first transmission structure, a second transmission structure, a first storage tank, and a second storage tank. The positioning mounting frame is bolted to the right side of the limiting support base. The reactor body is fixedly connected to the left side of the positioning mounting frame. The positioning connecting rod is rotatably connected to the left side of the reactor body, and damping is provided at the connection between the positioning connecting rod and the reactor body. The first transmission structure is located on the upper part of the limiting support base. The first storage tank is fixedly connected to the middle part of the positioning connecting rod. The second storage tank is bolted to the front side of the first storage tank. The second transmission structure is located on the upper part of the limiting support base.
[0007] Furthermore, the first transmission structure includes a reciprocating self-locking drive component and a transmission connecting shaft; the reciprocating self-locking drive component is bolted to the left side of the limiting support base; the transmission connecting shaft is rotatably connected to the left side of the limiting support base, and the transmission connecting shaft is coaxially and fixedly connected to the output shaft of the reciprocating self-locking drive component.
[0008] Furthermore, the first transmission structure also includes a position adjusting screw and a guide connecting rod; the position adjusting screw is rotatably connected to the upper part of the transmission connecting shaft, and the position adjusting screw is fixedly connected to the reactor body; multiple guide connecting rods are provided, and multiple guide connecting rods are circumferentially arrayed and fixedly connected to the upper end face of the transmission connecting shaft.
[0009] Furthermore, the first transmission structure also includes a driving gear, a guide connecting hole, and a second driven gear; the driving gear is threadedly connected to the outside of the position adjusting screw, and multiple guide connecting holes are provided, which are arranged in a circumferential array on the outside of the driving gear, and the multiple guide connecting holes are slidably connected to multiple guide connecting rods respectively; the second driven gear is fixedly connected to the lower side of the positioning connecting rod.
[0010] Furthermore, the second transmission structure includes a limiting double-ended lead screw and a first driven gear; the limiting double-ended lead screw is rotatably connected to the left side of the reactor body; the first driven gear is fixedly connected to the lower part of the limiting double-ended lead screw; the first driven gear meshes with the driving gear.
[0011] Furthermore, the second transmission structure also includes a limiting mounting ring and a rubber sealing ring; two limiting mounting rings are provided, and the two limiting mounting rings are respectively sleeved on the upper and lower sides of the reactor body, and the two limiting mounting rings are respectively threaded to the upper and lower sides of the limiting double-ended screw; two rubber sealing rings are provided, and the two rubber sealing rings are respectively bonded to the inner side of the two limiting mounting rings.
[0012] Compared with the prior art, the present invention has the following beneficial effects:
[0013] This invention avoids the inconvenience of disassembling the microreactor when replacing immobilized heparinase by replacing the first and second storage tanks, saving a lot of time and effectively improving the replacement efficiency of immobilized heparinase. It also reduces the downtime of the microreactor and improves the efficiency of the heparinase catalytic reaction. The microreactor is sealed with a rubber sealing ring, which effectively prevents leakage at the joint between the first or second storage tank and the reactor body, ensuring the normal operation of the microreactor and thus ensuring the heparinase catalytic effect. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0015] Figure 2 This is a schematic diagram showing the installation positions of the position adjusting screw, the limiting double-ended screw, and the positioning connecting rod of this utility model.
[0016] Figure 3 This is a schematic diagram showing the positional relationship between the first storage tank and the second storage tank of this utility model.
[0017] Figure 4 This is a schematic diagram showing the disassembled structure of the position adjustment screw, guide connecting rod, and active drive gear of this utility model.
[0018] Figure 5 This is a schematic diagram showing the positional relationship between the limiting double-ended lead screw, the first driven gear, and the limiting mounting ring of this utility model.
[0019] In the diagram, the correspondence between component names and drawing numbers is as follows:
[0020] 1. Limiting support base; 101. Reciprocating self-locking drive component; 102. Transmission connecting shaft; 103. Position adjusting screw; 104. Guide connecting rod; 105. Active drive gear; 106. Guide connecting hole; 107. Limiting double-ended screw; 108. First driven gear; 109. Limiting mounting ring; 110. Rubber sealing ring; 2. Positioning mounting frame; 3. Reactor body; 4. Positioning connecting rod; 401. Second driven gear; 5. First storage tank; 6. Second storage tank. Detailed Implementation
[0021] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples.
[0022] Example 1:
[0023] As attached Figure 1 To be continued Figure 4 As shown:
[0024] This invention provides a continuous flow microreactor for heparinase catalysis, comprising a limiting support base 1, a positioning mounting frame 2, a reactor body 3, a positioning connecting rod 4, a first transmission structure, a first storage tank 5, and a second storage tank 6; the positioning mounting frame 2 is bolted to the right side of the limiting support base 1; the reactor body 3 is fixedly connected to the left side of the positioning mounting frame 2; the positioning connecting rod 4 is rotatably connected to the left side of the reactor body 3, and damping is provided at the connection between the positioning connecting rod 4 and the reactor body 3; the first transmission structure is located on the upper part of the limiting support base 1; the first storage tank 5 is fixedly connected to the middle part of the positioning connecting rod 4; and the second storage tank 6 is bolted to the front side of the first storage tank 5.
[0025] The first transmission structure includes a reciprocating self-locking drive 101 and a transmission connecting shaft 102; the reciprocating self-locking drive 101 is bolted to the left side of the limiting support base 1; the transmission connecting shaft 102 is rotatably connected to the left side of the limiting support base 1, and the transmission connecting shaft 102 is coaxially and fixedly connected to the output shaft of the reciprocating self-locking drive 101.
[0026] The first transmission structure also includes a position adjusting screw 103 and a guide connecting rod 104; the position adjusting screw 103 is rotatably connected to the upper part of the transmission connecting shaft 102 and is fixedly connected to the reactor body 3; multiple guide connecting rods 104 are provided, and multiple guide connecting rods 104 are fixedly connected to the upper end face of the transmission connecting shaft 102 in a circumferential array.
[0027] The first transmission structure also includes an active drive gear 105, a guide connecting hole 106, and a second driven gear 401. The active drive gear 105 is threadedly connected to the outside of the position adjusting screw 103. Multiple guide connecting holes 106 are provided, and multiple guide connecting holes 106 are arranged in a circumferential array on the outside of the active drive gear 105. The multiple guide connecting holes 106 are slidably connected to multiple guide connecting rods 104 respectively. The second driven gear 401 is fixedly connected to the lower side of the positioning connecting rod 4.
[0028] The specific usage and function of this embodiment are as follows: Immobilized heparinase is added to the second storage tank 6, and then the reciprocating self-locking drive 101 is activated, causing the transmission connecting shaft 102 and the guide connecting rod 104 to drive the active drive gear 105 to rotate. At this time, the active drive gear 105 will move upward along the position adjustment screw 103. When the active drive gear 105 meshes with the second driven gear 401, the active drive gear 105 will drive the second driven gear 401 and the positioning connecting rod 4 to rotate. At this time, the first storage tank 5 will move out from the middle of the reactor body 3 until the second storage tank 6 is aligned with the reactor body 3. The guide connecting hole 106 and the guide connecting rod 104 can limit and guide the active drive gear 105, and the positioning mounting bracket 2 can fix the position of the reactor body 3.
[0029] Example 2:
[0030] As attached Figure 2 To be continued Figure 5 As shown:
[0031] Based on Embodiment 1, a second transmission structure is also included; the second transmission structure is disposed on the upper part of the limiting support base 1.
[0032] The second transmission structure includes a limiting double-ended lead screw 107 and a first driven gear 108; the limiting double-ended lead screw 107 is rotatably connected to the left side of the reactor body 3; the first driven gear 108 is fixedly connected to the lower part of the limiting double-ended lead screw 107; the first driven gear 108 meshes with the active drive gear 105.
[0033] The second transmission structure also includes a limiting mounting ring 109 and a rubber sealing ring 110. There are two limiting mounting rings 109, which are respectively sleeved on the upper and lower sides of the reactor body 3 and respectively threaded to the upper and lower sides of the limiting double-ended screw 107. There are two rubber sealing rings 110, which are respectively bonded to the inner side of the two limiting mounting rings 109.
[0034] The specific usage and function of this embodiment are as follows: When the first storage tank 5 is replaced with the second storage tank 6, the active drive gear 105 rotates and moves upward, simultaneously pushing the first driven gear 108 and the limiting double-ended screw 107 to rotate, causing the limiting mounting ring 109 to move to both sides. Only after the active drive gear 105 passes the first driven gear 108 and engages with the second driven gear 401 can the first storage tank 5 begin to operate. When the second storage tank 6 is aligned with the reactor body 3, manually rotating the limiting double-ended screw 107 will move the limiting mounting ring 109 back to its original position. When it is necessary to replace the second storage tank 6 with the first storage tank 6... Before tank 5, manually reverse the limiting double-ended screw 107 to remove the limiting installation ring 109. Then, reverse the reciprocating self-locking drive 101 to align the first storage tank 5 with the reactor body 3. At this time, the active drive gear 105 no longer meshes with the second driven gear 401. The reciprocating self-locking drive 101 continues to rotate so that the active drive gear 105 meshes with the first driven gear 108. At this time, the first driven gear 108 drives the limiting double-ended screw 107 to rotate, causing the limiting installation ring 109 to move back to the joint between the first storage tank 5 and the reactor body 3. The rubber sealing ring 110 will cover the joint to seal the reactor.
[0035] The following points should be noted in this article:
[0036] 1. The accompanying drawings of the embodiments disclosed herein only relate to the structures involved in the embodiments disclosed herein; other structures can be referred to in general design.
[0037] 2. Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.
[0038] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this disclosure should be included within the scope of protection of this disclosure. Therefore, the scope of protection of this disclosure should be determined by the scope of the claims.
Claims
1. A continuous flow microreactor for heparinase catalysis, comprising a limiting support base (1), a positioning mounting frame (2), a reactor body (3), a positioning connecting rod (4), a first transmission structure, a second transmission structure, a first storage tank (5), and a second storage tank (6); the positioning mounting frame (2) is bolted to the right side of the limiting support base (1); the reactor body (3) is fixedly connected to the left side of the positioning mounting frame (2); characterized in that: The positioning connecting rod (4) is rotatably connected to the left side of the reactor body (3), and damping is provided at the connection between the positioning connecting rod (4) and the reactor body (3); the first transmission structure is provided on the upper part of the limiting support base (1); the first storage tank (5) is fixedly connected to the middle part of the positioning connecting rod (4); the second storage tank (6) is bolted to the front side of the first storage tank (5); the second transmission structure is provided on the upper part of the limiting support base (1).
2. The continuous flow microreactor for heparinase-catalyzed reaction as described in claim 1, characterized in that: The first transmission structure includes a reciprocating self-locking drive (101) and a transmission connecting shaft (102); the reciprocating self-locking drive (101) is bolted to the left side of the limiting support base (1); the transmission connecting shaft (102) is rotatably connected to the left side of the limiting support base (1), and the transmission connecting shaft (102) is coaxially fixedly connected to the output shaft of the reciprocating self-locking drive (101).
3. The continuous flow microreactor for heparinase-catalyzed reaction as described in claim 2, characterized in that: The first transmission structure further includes a position adjusting screw (103) and a guide connecting rod (104); the position adjusting screw (103) is rotatably connected to the upper part of the transmission connecting shaft (102), and the position adjusting screw (103) is fixedly connected to the reactor body (3); multiple guide connecting rods (104) are provided, and multiple guide connecting rods (104) are circumferentially arrayed and fixedly connected to the upper end face of the transmission connecting shaft (102).
4. The continuous flow microreactor for heparinase-catalyzed reaction as described in claim 3, characterized in that: The first transmission structure further includes a drive gear (105), a guide connecting hole (106), and a second driven gear (401); the drive gear (105) is threaded to the outside of the position adjusting screw (103); multiple guide connecting holes (106) are provided, and multiple guide connecting holes (106) are arranged in a circumferential array on the outside of the drive gear (105); the multiple guide connecting holes (106) are slidably connected to multiple guide connecting rods (104); the second driven gear (401) is fixedly connected to the lower side of the positioning connecting rod (4).
5. The continuous flow microreactor for heparinase-catalyzed reaction as described in claim 4, characterized in that: The second transmission structure includes a limiting double-ended lead screw (107) and a first driven gear (108); the limiting double-ended lead screw (107) is rotatably connected to the left side of the reactor body (3); the first driven gear (108) is fixedly connected to the lower part of the limiting double-ended lead screw (107); the first driven gear (108) meshes with the active drive gear 1 (05).
6. The continuous flow microreactor for heparinase-catalyzed reaction as described in claim 5, characterized in that: The second transmission structure also includes a limiting mounting ring (109) and a rubber sealing ring (110); two limiting mounting rings (109) are provided, and the two limiting mounting rings (109) are respectively sleeved on the upper and lower sides of the reactor body (3), and the two limiting mounting rings (109) are respectively threaded to the upper and lower sides of the limiting double-ended screw (107); two rubber sealing rings (110) are provided, and the two rubber sealing rings (110) are respectively bonded to the inner side of the two limiting mounting rings (109).