Dendrobium officinale protocorm genetic transformation culture device
By designing a device consisting of an Erlenmeyer flask and a transformation culture flask, the problems of contamination and complex operation in the genetic transformation culture of Dendrobium officinale protocorms were solved, and simplified operation and efficient culture medium management were achieved under sterile conditions.
Patent Information
- Application Number
- CN202520177661.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-27
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2035-01-27
AI Technical Summary
The genetic transformation culture of Dendrobium officinale protocorms is prone to contamination and the operation is complicated, making it difficult to perform operations such as changing the culture medium and cleaning in a sterile environment.
Design an apparatus consisting of a triangular flask and a transformation culture flask. The transformation culture flask is composed of an inclined body, a vertical body, and a top body. Combined with an elastic sealing ring and an adjustable cap, it can achieve solid-liquid separation and gas exchange, avoid contamination, and simplify operation.
The process of changing the culture medium and cleaning can be completed without removing the original bulb, avoiding contamination, simplifying the operation process, and improving the sterility and efficiency of the culture.
Smart Images
Figure CN223844603U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to a kind of transformation culture devices. BACKGROUND
[0002] Dendrobium candidum is a traditional Chinese medicine, and it has the effects of nourishing yin and stomach, clearing heat and generating saliva, and moistening lung and relieving cough. However, Dendrobium candidum has very strict requirements for the environment. Under natural conditions, the seed germination rate is very low, and the growth is very slow. In addition, due to over-exploitation, environmental degradation and other factors, the natural resources of Dendrobium candidum are decreasing. In order to protect and utilize this rare Chinese medicinal material and increase production, many scholars have conducted research on the rapid propagation of Dendrobium candidum tissue culture and have made certain progress. In order to obtain high content of Dendrobium candidum polysaccharide and other effective components such as alkaloids, the source and application of Dendrobium candidum are expanded. The transformation culture of protocorm can be used to promote the genetic transformation efficiency of protocorm and improve the growth rate of protocorm, which is a commonly used technology in plant tissue culture. Dendrobium candidum protocorm is a fertile embryo formed during the aseptic seed germination process of wild Dendrobium candidum fruit pods. Transformation culture is to inoculate protocorm into agrobacterium resuspension liquid containing exogenous genes, and to shake culture in a constant temperature incubator at a suitable temperature and environment. With the passage of time, protocorm will gradually proliferate and eventually form Dendrobium candidum sprouts. The time of transformation culture is affected by many factors, including growth rate, composition of culture medium, setting of culture conditions, etc. Generally, the time required for transformation culture is between 3-5 days. During the transformation culture process, strict control of contamination is required, and it needs to be carried out in a sterile environment to ensure the sterility of the culture process and obtain high-quality Dendrobium candidum sprouts. During the culture process, the growth of protocorm needs to be observed regularly, and the culture medium needs to be replaced in time to ensure the supply of nutrients. It also needs to be replaced regularly or washed before neutralization reaction. The transformation culture of protocorm is generally carried out by placing protocorm in a triangular culture bottle, and the bottle opening is covered with a gas permeable membrane to prevent protocorm from leaking from the bottle opening during shaking in the incubator. However, this device is prone to accidental contamination during the replacement of resuspension liquid, washing, watering or transfer process, and the operation is complex, which requires more other instruments to cooperate. UTILIZY MODEL CONTENT
[0003] The utility model discloses a kind of Dendrobium candidum protocorm genetic transformation culture devices to solve the problem that pollution easily occurs in the genetic transformation culture process of existing Dendrobium candidum protocorm.
[0004] The utility model discloses a dendrobium candidum protocorm genetic transformation culture device is formed by triangular bottle (1) and transformation culture bottle, and transformation culture bottle sets up in the triangular bottle (1), and transformation culture bottle is formed by inclined bottle body (2), vertical bottle body (3), top bottle body (4) and bottle cap (9), and the bottom of inclined bottle body (2) is sealed, and the upper end of inclined bottle body (2) is fixedly connected with vertical bottle body (3), and vertical bottle body (3) is vertically arranged, and the lower end of top bottle body (4) is arranged in the upper end inside of vertical bottle body (3), and the upper end of top bottle body (4) is provided with bottle cap (9), and the lateral wall of bottle cap (9) is opened with strip hole (11), and the lateral wall of bottle cap (9) is threadedly connected with the upper portion of top bottle body (4), and a plurality of drain holes (10) are opened on the lateral wall of top bottle body (4) in strip hole (11), and the lower end outer wall of vertical bottle body (3) and the bottle mouth inner wall of triangular bottle (1) are provided with first elastic sealing ring (5).
[0005] The utility model discloses a dendrobium candidum protocorm genetic transformation culture device is formed by triangular bottle (1) and transformation culture bottle, and transformation culture bottle sets up in the triangular bottle (1), and transformation culture bottle is formed by inclined bottle body (2), vertical bottle body (3), top bottle body (4) and bottle cap (9), and the bottom of inclined bottle body (2) is sealed, and the upper end of inclined bottle body (2) is fixedly connected with vertical bottle body (3), and vertical bottle body (3) is vertically arranged, and the lower end of top bottle body (4) is arranged in the upper end inside of vertical bottle body (3), and the upper end of top bottle body (4) is provided with bottle cap (9), and the lateral wall of bottle cap (9) is opened with strip hole (11), and the lateral wall of bottle cap (9) is threadedly connected with the upper portion of top bottle body (4), and a plurality of drain holes (10) are opened on the lateral wall of top bottle body (4) in strip hole (11), and the lower end outer wall of vertical bottle body (3) and the bottle mouth inner wall of triangular bottle (1) are provided with first elastic sealing ring (5).
[0006] The utility model discloses a dendrobium candidum protocorm genetic transformation culture device is formed by triangular bottle (1) and transformation culture bottle, and transformation culture bottle sets up in the triangular bottle (1), and transformation culture bottle is formed by inclined bottle body (2), vertical bottle body (3), top bottle body (4) and bottle cap (9), and the bottom of inclined bottle body (2) is sealed, and the upper end of inclined bottle body (2) is fixedly connected with vertical bottle body (3), and vertical bottle body (3) is vertically arranged, and the lower end of top bottle body (4) is arranged in the upper end inside of vertical bottle body (3), and the upper end of top bottle body (4) is provided with bottle cap (9), and the lateral wall of bottle cap (9) is opened with strip hole (11), and the lateral wall of bottle cap (9) is threadedly connected with the upper portion of top bottle body (4), and a plurality of drain holes (10) are opened on the lateral wall of top bottle body (4) in strip hole (11), and the lower end outer wall of vertical bottle body (3) and the bottle mouth inner wall of triangular bottle (1) are provided with first elastic sealing ring (5). BRIEF DESCRIPTION OF DRAWINGS
[0007] Fig. 1 It is the structure schematic diagram of the dendrobium candidum protocorm genetic transformation culture device in example 1;
[0008] Fig. 2 It is the structure schematic diagram of top bottle body (4).
[0009] Fig. 3 It is the structure schematic diagram of bottle cap (9). DETAILED DESCRIPTION
[0010] The technical scheme of the utility model is not limited to the following specific embodiments, and also includes any reasonable combination of the specific embodiments.
[0011] Specific embodiment one: the genetic transformation culture device for Dendrobium candidum protocorm of the embodiment is composed of a triangular flask (1) and a transformation culture flask; the transformation culture flask is arranged in the triangular flask (1); the transformation culture flask is composed of an inclined bottle body (2), a vertical bottle body (3), a top bottle body (4) and a bottle cap (9); the inclined bottle body (2) is an inclined tube, the bottom of the inclined bottle body (2) is sealed, the upper end of the inclined bottle body (2) is fixedly connected with the vertical bottle body (3), the vertical bottle body (3) is arranged vertically, the lower end of the top bottle body (4) is arranged inside the upper end of the vertical bottle body (3), and the upper end of the top bottle body (4) is provided with the bottle cap (9); a strip-shaped hole (11) is formed in the side wall of the bottle cap (9), the side wall of the bottle cap (9) is threadedly connected with the upper part of the top bottle body (4), and a plurality of drainage holes (10) are formed in the side wall of the top bottle body (4) in the strip-shaped hole (11); a first elastic sealing ring (5) is arranged between the lower end outer wall of the vertical bottle body (3) and the inner wall of the bottle mouth of the triangular flask (1).
[0012] The genetic transformation culture device for Dendrobium candidum protocorm of the embodiment is used for genetic transformation culture of Dendrobium candidum protocorm, the inclined bottle body (2) is arranged in an inclined manner, and the Dendrobium candidum protocorm can be prevented from leaking from the bottle mouth during shaking of the shaking table. The vertical bottle body (3) is used for being connected with the triangular flask (1), and the first elastic sealing ring (5) is used for sealing the vertical bottle body (3) and the triangular flask (1). The side wall of the top bottle body (4) is provided with a plurality of drainage holes (10), the top bottle body (4) is in an open state when the strip-shaped hole (11) in the bottle cap (9) is aligned with the plurality of drainage holes (10), the Dendrobium candidum protocorm enters the top bottle body (4) after the transformation culture device is inverted, solid-liquid separation can be realized, the bottle cap (9) closes the whole transformation culture device when the strip-shaped hole (11) in the bottle cap (9) is misaligned with the plurality of drainage holes (10), and the plurality of drainage holes (10) are used for gas exchange between the inside of the device and the outside when the transformation culture device is arranged in a normal position. The genetic transformation culture device for Dendrobium candidum protocorm of the embodiment can complete the operations such as replacement of culture solution, replacement of resuspension liquid or neutralization reaction process of the Dendrobium candidum protocorm, cleaning and the like without leaving the transformation culture device, pollution can be avoided, and the operation is simple and does not need many other instruments to cooperate.
[0013] Specific embodiment two: different from the specific embodiment one, an outer wall of the bottle cap (9) is provided with a third handle (12).
[0014] Specific embodiment three: different from the specific embodiment one or two, the third handle (12) is perpendicularly fixedly connected with the outer wall of the bottle cap (9).
[0015] Specific implementation four: the difference between this implementation and one of the specific implementations one to three is that the outer wall of the vertical bottle body (3) is provided with a first handle (7).
[0016] Specific implementation five: the difference between this implementation and one of the specific implementations one to four is that the first handle (7) is vertically fixed to the outer wall of the vertical bottle body (3).
[0017] Specific implementation six: the difference between this implementation and one of the specific implementations one to five is that the outer wall of the top bottle body (4) is provided with a second handle (8).
[0018] Specific implementation seven: the difference between this implementation and one of the specific implementations one to six is that the second handle (8) is vertically fixed to the outer wall of the top bottle body (4).
[0019] Specific implementation eight: the difference between this implementation and one of the specific implementations one to seven is that a second elastic sealing ring (6) is arranged between the lower end outer wall of the top bottle body (4) and the upper end inner wall of the vertical bottle body (3).
[0020] Specific implementation nine: the difference between this implementation and one of the specific implementations one to eight is that the diameter of the drainage hole (10) is 1.5 mm.
[0021] Specific implementation ten: the difference between this implementation and one of the specific implementations one to nine is that the drainage hole (10) is arranged in a rectangular array.
[0022] Example 1
[0023] In combination Figs. 1-3To illustrate the embodiment, the Dendrobium candidum protocorm genetic transformation culture device in the embodiment is composed of a triangular flask (1) and a transformation culture flask; the transformation culture flask is arranged in the triangular flask (1); the transformation culture flask is composed of an inclined bottle body (2), a vertical bottle body (3), a top bottle body (4) and a bottle cap (9); the inclined bottle body (2) is an inclined tube, the bottom of the inclined bottle body (2) is sealed, the upper end of the inclined bottle body (2) is fixedly connected with the vertical bottle body (3), the vertical bottle body (3) is arranged vertically, the lower end of the top bottle body (4) is arranged inside the upper end of the vertical bottle body (3), a second elastic sealing ring (6) is arranged between the outer wall of the lower end of the top bottle body (4) and the inner wall of the upper end of the vertical bottle body (3), and the upper end of the top bottle body (4) is provided with the bottle cap (9); a strip-shaped hole (11) is formed in the side wall of the bottle cap (9), the side wall of the bottle cap (9) is threadedly connected with the upper part of the top bottle body (4), a plurality of drainage holes (10) arranged in a rectangular array are formed in the side wall of the top bottle body (4) inside the strip-shaped hole (11), and the diameter of the drainage holes (10) is 1.5 mm; a first elastic sealing ring (5) is arranged between the outer wall of the lower end of the vertical bottle body (3) and the inner wall of the bottle mouth of the triangular flask (1); a third handle (12) is arranged on the outer wall of the bottle cap (9), and the third handle (12) is perpendicularly fixedly connected with the outer wall of the bottle cap (9); a first handle (7) is arranged on the outer wall of the vertical bottle body (3), and the first handle (7) is perpendicularly fixedly connected with the outer wall of the vertical bottle body (3); a second handle (8) is arranged on the outer wall of the top bottle body (4), and the second handle (8) is perpendicularly fixedly connected with the outer wall of the top bottle body (4); the Dendrobium candidum protocorm genetic transformation culture device in the embodiment is used for Dendrobium candidum protocorm genetic transformation culture, the inclined bottle body (2) is arranged obliquely, and the protocorm can be prevented from leaking from the bottle mouth during shaking of the shaking table. The vertical bottle body (3) is used for being connected with the triangular flask (1), and the first elastic sealing ring (5) is used for sealing the vertical bottle body (3) and the triangular flask (1). A plurality of drainage holes (10) are formed in the side wall of the top bottle body (4), the top bottle body (4) is in an open state when the strip-shaped hole (11) in the bottle cap (9) is aligned with the plurality of drainage holes (10), the protocorm enters the top bottle body (4) after the transformation culture device is inverted, solid-liquid separation can be realized, the bottle cap (9) closes the entire transformation culture device when the strip-shaped hole (11) in the bottle cap (9) is misaligned with the plurality of drainage holes (10), and the plurality of drainage holes (10) are used for gas exchange between the inside of the device and the outside when the transformation culture device is placed upright. The Dendrobium candidum protocorm genetic transformation culture device in the embodiment can complete the operations of replacing a culture solution, replacing a resuspension solution or performing a neutralization reaction process protocorm cleaning without the protocorm leaving the transformation culture device, pollution can be avoided, and the operations are simple and do not need many other instruments to cooperate.
Claims
1. A genetic transformation culture device for Dendrobium officinale protocorms, characterized in that: The device for genetic transformation culture of Dendrobium candidum protocorm is composed of a triangular bottle (1) and a transformation culture bottle; the transformation culture bottle is arranged in the triangular bottle (1); the transformation culture bottle is composed of an inclined bottle body (2), a vertical bottle body (3), a top bottle body (4) and a bottle cap (9); the inclined bottle body (2) is an inclined tube, the bottom of the inclined bottle body (2) is sealed, the upper end of the inclined bottle body (2) is fixedly connected with the vertical bottle body (3), the vertical bottle body (3) is arranged vertically, the lower end of the top bottle body (4) is arranged inside the upper end of the vertical bottle body (3), the upper end of the top bottle body (4) is provided with the bottle cap (9); a strip-shaped hole (11) is formed in the side wall of the bottle cap (9), the side wall of the bottle cap (9) is threadedly connected with the upper part of the top bottle body (4), a plurality of drainage holes (10) are formed in the side wall of the top bottle body (4) inside the strip-shaped hole (11); a first elastic sealing ring (5) is arranged between the lower end outer wall of the vertical bottle body (3) and the inner wall of the bottle mouth of the triangular bottle (1).
2. The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: The outer wall of the bottle cap (9) is provided with a third handle (12). 3.The Dendrobium candidum protocorm genetic transformation culture device according to claim 2, characterized in that: The third handle (12) is perpendicularly fixedly connected with the outer wall of the bottle cap (9).
4. The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: The outer wall of the vertical bottle body (3) is provided with a first handle (7). 5.The Dendrobium candidum protocorm genetic transformation culture device according to claim 4, characterized in that: The first handle (7) is perpendicularly fixedly connected with the outer wall of the vertical bottle body (3). 6.The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: The outer wall of the top bottle body (4) is provided with a second handle (8).
7. The Dendrobium candidum protocorm genetic transformation culture device according to claim 6, characterized in that: The second handle (8) is perpendicularly fixedly connected with the outer wall of the top bottle body (4). 8.The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: A second elastic sealing ring (6) is arranged between the lower end outer wall of the top bottle body (4) and the upper end inner wall of the vertical bottle body (3). 9.The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: The diameter of the drainage hole (10) is 1.5 mm. 10.The Dendrobium candidum protocorm genetic transformation culture device according to claim 1, characterized in that: The drainage holes (10) are arranged in a rectangular array.