Raw material dissolving device for preparing CHO cell transfection protein culture medium

Abstract

The utility model discloses a raw material dissolving device for preparing a CHO cell transfection protein culture medium. The raw material dissolving device comprises a tank body, a feeding opening formed in one side of the top end of the tank body, and a motor connected to the top end of the tank body, the auxiliary feeding structure comprises a feeding chamber fixed at the top end of the feeding port; when feeding needs to be carried out in the midway of a dissolution reaction, feeding can be carried out in the tank body through the auxiliary feeding structure, and a worker only needs to open the movable cover, feed a reagent into the feeding opening, enter the feeding chamber and fall on the partition plate, then close the movable cover and pull the partition plate, so that the reagent can be fed into the tank body through the auxiliary feeding structure. The reagent on the surface of the partition plate is scraped off by utilizing the scraper, and the reagent enters the tank body to complete feeding, so that the design has the advantages that in the whole feeding process, a stable environment in the tank body can be well maintained, subsequent reaction is facilitated, and meanwhile, external dust can be prevented from falling into the tank body during feeding by utilizing multiple protection, so that the feeding efficiency is improved. Raw material pollution is prevented.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of dissolving tank, specifically relates to a raw material dissolving device for CHO cell transfected protein culture medium preparation. BACKGROUND

[0002] CHO cell is a kind of commonly used mammalian cell line, has easy to cultivate, strong stability, strong post-translational modification ability to protein etc. These characteristics make CHO cell be widely applied in biological medicine and vaccine production, including producing monoclonal antibody, recombinant protein, growth factor etc. Protein medicine, CHO cell transfected protein refers to the process that the exogenous DNA or RNA is introduced into CHO (Chinese hamster ovary) cell by specific technical means, and the corresponding protein is expressed;CHO cell transfected protein culture medium is a kind of culture medium specially designed for Chinese hamster ovary cell (CHO cell) transfected protein;The preparation of CHO cell transfected protein culture medium is a complex process, and the dissolution of raw material is one of key steps, and the dissolving tank is a kind of device commonly used when carrying out dissolution step, and the dissolving tank is usually a large stainless steel or glass container, is equipped with heating, cooling and stirring system. There can be a sealing cover on the upper portion of the container for preventing solution from volatilizing and being contaminated, raw materials are put into the dissolving tank together with various reagents, and the inside of the tank is promoted by stirring, temperature rising, pressure rising and various ways.

[0003] In the dissolution process, all reagents are not added into the dissolving tank at one time, sometimes various reagents need to be added into the inside of the dissolving tank in stages and batches, and when adding, the dissolving tank needs to be opened, which has certain disadvantages, the inside of the dissolving tank has been reacting, and the temperature environment in the inside is in a relatively stable state, directly opening the dissolving tank can destroy the stable environment in the inside of the tank, is not conducive to the continuous reaction, and after opening the dissolving tank, external dust impurities can easily fall into the inside of the tank, so that the raw materials are polluted, therefore, a new dissolving tank needs to be designed to solve the problem. UTILITY MODEL CONTENTS

[0004] The utility model aims at providing a raw material dissolving device for CHO cell transfected protein culture medium preparation to solve the problems that the stable environment in the inside of the tank is easily destroyed and the inside raw materials are easily polluted when adding reagents in the middle of the above background technology.

[0005] To achieve the above object, the utility model provides the following technical scheme: a raw material dissolving device for CHO cell transfected protein culture medium preparation, including

[0006] Tank body, the feeding port being arranged at one side of the top end of the tank body, the motor being connected at the top end of the tank body,

[0007] The auxiliary feeding structure comprises a feeding chamber fixed at the top end of the feeding port, a feeding port connected at one side of the feeding chamber, a movable cover rotatably connected at the top end of the feeding port, a feeding groove penetratingly arranged between the feeding chamber and the feeding port, a partition plate connected to the feeding chamber, and a scraper fixed to the inner wall of the feeding chamber and tightly abutting the surface of the partition plate at the bottom end.

[0008] Preferably, the partition plate is in an L-shaped structure, the inner end of the partition plate covers the inside of the feeding chamber, and the outer end of the partition plate extends out of the sliding installation groove arranged at one side of the feeding chamber, and the partition plate is slidingly connected with the sliding installation groove.

[0009] Preferably, the auxiliary feeding structure further comprises a spring, and the spring is fixedly connected between the outer end of the partition plate and the outer surface of the feeding chamber.

[0010] Preferably, the inside of the feeding chamber is communicated with the inside of the tank body, and the movable cover is rotatably connected at the top end of the feeding port by a torsion spring.

[0011] Preferably, the auxiliary feeding structure further comprises a rubber sealing gasket, and the rubber sealing gasket is arranged on the inner wall of the feeding chamber and located at the position of the end port of the sliding installation groove.

[0012] Preferably, the top end of the feeding chamber is provided with a top cover assembly, the top cover assembly comprises a top port, a cover body and a bolt, the top port is arranged at the top end surface of the feeding chamber, and the cover body is fixedly installed in the inside of the top port by the bolt.

[0013] Preferably, the top end of the tank body is provided with an exhaust assembly, the exhaust assembly comprises an exhaust pipe, an end head, a threaded connector and a rubber pad, the inner end of the exhaust pipe extends into the inside of the tank body, the end head is fixedly connected at the top end of the exhaust pipe, the threaded connector is connected at the surface of the end head, and the rubber pad is fixed at the surface of the end head and sleeved outside the threaded connector.

[0014] Compared with the prior art, the auxiliary feeding structure has the following beneficial effects:

[0015] When feeding is needed in the middle of the dissolution reaction, the auxiliary feeding structure can be used to feed into the inside of the tank body, a person only needs to open the movable cover and put the reagent into the inside of the feeding port, the reagent falls on the partition plate after entering the inside of the feeding chamber, then the movable cover is closed and the partition plate is pulled out, and the reagent on the surface of the partition plate is scraped down by the scraper, at this time, the reagent enters the inside of the tank body and the feeding is completed, and the design has the advantages that the stable environment in the inside of the tank body can be maintained during the whole feeding process, which is beneficial to the subsequent reaction, and the external dust can be prevented from falling into the inside of the tank body during feeding by using multiple blocking and protection, so that the raw materials are prevented from being polluted. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1It is a three-dimensional schematic view of the utility model;

[0017] Figure 2 It is a three-dimensional schematic view of the auxiliary feeding structure of the utility model;

[0018] Figure 3 It is a front view and sectional view of the auxiliary feeding structure of the utility model;

[0019] Figure 4 It is the utility model Figure 3 It is an enlarged schematic view of area A;

[0020] Figure 5 It is a front view and sectional view of the exhaust assembly of the utility model;

[0021] In the figure: 100, tank body; 200, feeding port; 300, motor; 400, auxiliary feeding structure; 401, feeding chamber; 402, feeding port; 403, movable cover; 404, partition; 405, top cover assembly; 4051, top port; 4052, cover body; 4053, bolt; 406, feeding groove; 407, scraper; 408, sliding installation groove; 409, spring; 4010, rubber sealing gasket; 500, exhaust assembly; 501, exhaust pipe; 502, end; 503, threaded connector; 504, rubber pad. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0023] EMBODIMENT

[0024] Please refer to Figures 1 to 5 For the embodiments of the utility model, the embodiment provides a technical scheme: a CHO cell transfection protein culture medium preparation raw material dissolving device, comprising

[0025] The tank body 100, the feeding port 200 arranged at one side of the top end of the tank body 100 and the motor 300 connected to the top end of the tank body 100, the feeding port 200 can be used for initial feeding, the tank body 100 is internally provided with a stirring rod, and the motor 300 can drive the stirring rod to stir the raw materials in the tank body 100;

[0026] The auxiliary feeding structure 400 comprises a feeding chamber 401 fixed at the top end of the feeding port 200, a feeding port 402 connected at one side of the feeding chamber 401, a movable cover 403 rotatably connected at the top end of the feeding port 402, a feeding groove 406 penetratingly arranged between the feeding chamber 401 and the feeding port 402, a partition plate 404 connected on the feeding chamber 401, a scraping plate 407 fixed on the inner wall of the feeding chamber 401 and closely attached to the surface of the partition plate 404 at the bottom end, and the auxiliary feeding structure 400 is used for feeding the reagent into the tank 100 during the working process. The bottom surface of the feeding port 402 is inclined, so that the reagent can smoothly fall down. The auxiliary feeding structure 400 has the advantages that the stable environment in the tank 100 can be maintained during the whole feeding process, which is beneficial to the subsequent reaction, and the external dust can be prevented from falling into the tank 100 during the feeding process by using the multiple blocking and protecting structures, thereby preventing the pollution of the raw materials.

[0027] In the embodiment, preferably, the partition plate 404 has an L-shaped structure, the inner end of the partition plate 404 covers the inside of the feeding chamber 401, and the reagent fed from the feeding port 402 falls on the partition plate 404. The outer end of the partition plate 404 extends out of the sliding installation groove 408 arranged on one side of the feeding chamber 401, and the partition plate 404 is slidingly connected with the sliding installation groove 408 and can be pulled out and slid in the sliding installation groove 408.

[0028] In the embodiment, preferably, the auxiliary feeding structure 400 further comprises a spring 409 fixedly connected between the outer end of the partition plate 404 and the outer surface of the feeding chamber 401. When the partition plate 404 is pulled outwards, the spring 409 is pulled, and then the spring 409 is used to drive the partition plate 404 to reset.

[0029] In the embodiment, preferably, the inside of the feeding chamber 401 is communicated with the inside of the tank 100, and the movable cover 403 is rotatably connected at the top end of the feeding port 402 by a torsion spring. In the normal state, the movable cover 403 can always cover the top end of the feeding port 402.

[0030] In the embodiment, preferably, the auxiliary feeding structure 400 further comprises a rubber sealing gasket 4010 arranged on the inner wall of the feeding chamber 401 and located at the position of the sliding installation groove 408. The rubber sealing gasket 4010 can seal to prevent the reagent from leaking from the periphery of the partition plate 404 and the sliding installation groove 408 when the partition plate 404 is not opened.

[0031] In the embodiment, preferably, the top end of the feeding chamber 401 is provided with a top cover assembly 405, the top cover assembly 405 comprises a top opening 4051, a cover body 4052 and a bolt 4053, the top opening 4051 is arranged on the top end surface of the feeding chamber 401, the cover body 4052 is fixedly installed in the inside of the top opening 4051 through the bolt 4053, and personnel can disassemble the bolt 4053 so as to disassemble the cover body 4052, thereby regularly cleaning the auxiliary feeding structure 400.

[0032] In the embodiment, preferably, the top end of the feeding chamber 401 is provided with a top cover assembly 405, the top cover assembly 405 comprises a top opening 4051, a cover body 4052 and a bolt 4053, the top opening 4051 is arranged on the top end surface of the feeding chamber 401, the cover body 4052 is fixedly installed in the inside of the top opening 4051 through the bolt 4053, and personnel can disassemble the bolt 4053 so as to disassemble the cover body 4052, thereby regularly cleaning the auxiliary feeding structure 400.

[0033] Working principle: when it is needed to add reagent or material into the inside of the tank in the middle of the working process of the dissolving tank, the movable cover can be first lifted, and the reagent can be added into the inside of the feeding opening, the reagent falls on the surface of the baffle from the feeding groove, then the movable cover is closed to cover the feeding opening, personnel can pull the baffle, the baffle is gradually pulled out from the sliding installation groove, at this time, the bottom end of the scraper can scrape the reagent on the surface of the rubber sealing gasket and drop into the inside of the tank, the adding of the reagent is completed, then the baffle is loosened, the baffle is reset under the rebound of the spring, and covers the inside of the feeding chamber again for the next time of feeding.

[0034] Although the embodiments of the utility model have been shown and described (detailed in the above detailed description), it can be understood by those skilled in the art that the embodiments can be changed, modified, replaced and changed in various ways without departing from the principles and spirits of the utility model, the range of the utility model is defined by the appended claims and its equivalents.

Claims

1. A CHO cell transfection protein culture medium preparation raw material dissolving device, characterized by: The utility model provides a kind of auxiliary feeding structure of tank, which belongs to the technical field of tank feeding device. The auxiliary feeding structure includes a tank body (100), a feeding port (200) arranged on one side of the top end of the tank body (100), and a motor (300) connected to the top end of the tank body (100). The auxiliary feeding structure further includes a spring (409) fixedly connected between the outer end of the partition plate (404) and the outer surface of the feeding chamber (401).

2. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 1, characterized in that: The feeding chamber (401) is in communication with the inside of the tank body (100), and the movable cover (403) is rotatably connected to the top end of the feeding port (402) by a torsion spring.

3. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 2, characterized in that: The auxiliary feeding structure (400) further includes a rubber sealing gasket (4010) arranged on the inner wall of the feeding chamber (401) and located at the position of the sliding installation groove (408).

4. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 3, characterized in that: The top end of the feeding chamber (401) is provided with a top cover assembly (405), which includes a top opening (4051), a cover body (4052), and a bolt (4053). The top opening (4051) is arranged on the top end surface of the feeding chamber (401), and the cover body (4052) is fixedly installed in the inside of the top opening (4051) by the bolt (4053).

5. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 4, characterized in that: The top end of the tank body (100) is provided with an exhaust assembly (500), which includes an exhaust pipe (501), an end head (502), a threaded connector (503), and a rubber pad (504). The inner end of the exhaust pipe (501) extends into the inside of the tank body (100), the end head (502) is fixedly connected to the top end of the exhaust pipe (501), the threaded connector (503) is connected to the surface of the end head (502), and the rubber pad (504) is fixedly connected to the surface of the end head (502) and wrapped around the outside of the threaded connector (503).

6. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 5, characterized in that: ​ 7. The CHO cell transfection protein culture medium preparation raw material dissolving device according to claim 6, characterized in that: ​

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