Device for refitting closed container into high-density cell bioreactor

Abstract

The utility model discloses a device for refitting a closed container into a high-density cell bioreactor, and relates to the technical field of water body ecological restoration. The device comprises a permeation part, a filtering part and a fixing part which are detachably connected, the permeation part comprises a semi-permeable membrane fixing disc and a first connecting part which are of an integrated structure, the semi-permeable membrane fixing disc is arranged in a closed container, and the first connecting part is connected with the fixing part and the filtering part outside the closed container through a through hole of the closed container. According to the device for refitting the closed container into the high-density cell bioreactor disclosed by the utility model, the closed container has a high-density cell culture function by arranging the filtering and permeation-permitting additional component in the closed container; the problems that an existing traditional microalgae culture device is poor in adaptability, and the algal bloom treatment technology is high in cost, low in efficiency, high in energy consumption and the like can be solved.

Description

Technical Field

[0001] This utility model relates to the field of water body ecological restoration technology, and in particular to a device for modifying a sealed container into a high-density cell bioreactor. Background Technology

[0002] Under the dual impact of human activities and climate change, the problem of eutrophication of large lakes and reservoirs around the world is becoming increasingly serious. The sixth UN Sustainable Development Goal is to ensure that "by 2030, everyone shall have universal and equitable access to safe and affordable drinking water."

[0003] In recent years, harmful algal blooms (HABs) have occurred frequently around the world, posing a threat to aquatic ecosystems and human health. According to the Harmful Algae Database (HAEDAT), more than 200 countries and regions worldwide experience harmful algal blooms each year, leading to a series of environmental, social, and economic problems.

[0004] Currently, the main methods for treating cyanobacterial blooms include physical methods, chemical methods, and biological methods.

[0005] (1) Physical methods: These mainly involve methods such as harvesting, filtration, and ultrasonic algae removal. However, these methods are time-consuming, labor-intensive, costly, and energy-intensive, and can easily lead to the release of algal toxins. They also cannot fundamentally solve the problem of cyanobacterial blooms. Physical methods require a large amount of human and material resources and may have negative impacts on benthic organisms. They should be considered as a preventative measure rather than a control measure.

[0006] (2) Chemical methods: chemical algaecides, flocculants, etc. However, the widespread use of algaecides is not only costly, but also poses a potential threat to human health and the aquatic environment.

[0007] (3) Biological methods: including competition with aquatic plants, algae-eating organisms, and microbial decomposition. These methods have relatively low environmental risks but are less efficient and can easily attract alien species.

[0008] A method for preventing and controlling algal blooms in eutrophic water bodies (application number: 202010727273.1) relates to an algal bloom suppression device, specifically an ecological interference device that inhibits algal blooms by suppressing light energy and carbon dioxide absorption, thereby inhibiting the photosynthesis of harmful algae. By using a semi-permeable membrane, a bioreactor is placed in the eutrophic water body, simultaneously achieving microalgae cultivation and competitive suppression of algal blooms. However, this device has a complex structure and high manufacturing cost, hindering its large-scale application. Utility Model Content

[0009] In order to solve the above problems, the utility model provides a device for refitting closed container to high density cell bioreactor, through install a filter and allow the additional component of permeation in the closed container, thereby make the closed container have the function of high density cell culture, can solve the problem of poor adaptability of current traditional microalgae culture device and high cost, low efficiency, high energy consumption etc.

[0010] In order to achieve the above object, the utility model provides a device for refitting closed container to high density cell bioreactor, including detachable connection's permeation part, filter part and fixed part, permeation part includes semipermeable membrane fixed disc of integral structure and first connecting part, semipermeable membrane fixed disc sets up in the closed container inside, and first connecting part is connected with the fixed part, filter part outside the closed container through the through -hole of closed container.

[0011] Preferably, first connecting part is hollow cylinder with external thread, and semipermeable membrane is fixed in the inner ring center of semipermeable membrane fixed disc.

[0012] Preferably, semipermeable membrane fixed disc is provided with a plurality of spiral grooves on the side away from first connecting part.

[0013] Preferably, filter part includes filter disc and second connecting part of integral structure, and second connecting part is hollow cylinder with internal thread, and the internal thread of second connecting part is matched with the external thread of first connecting part.

[0014] Preferably, a plurality of filter holes are formed in filter disc, and the diameter of filter holes is 12mm-45mm.

[0015] Preferably, the center of fixed part is provided with a threaded hole matched with the external thread of first connecting part.

[0016] Preferably, the device is located at the interface between the refitted high density cell bioreactor and external liquid, and the permeation part is immersed or at least half-immersed in the external liquid.

[0017] The device for refitting closed container to high density cell bioreactor has the following advantages:

[0018] (1) by installing and fixing the assembly with the functions of filtering and permeation into the closed container, the closed container has the function of high density cell bioreactor, simplifies the structure of high density cell bioreactor, reduces the connection of components, effectively reduces the maintenance cost, and improves the stability and service life of high density cell bioreactor;

[0019] (2)The modified high-density cell bioreactor has the functions of traditional high-density cell bioreactor microalgae culture and algal bloom inhibition, and can simultaneously meet the needs of microalgae high-density growth and algal bloom control, thereby improving the culture effect;

[0020] (3)By using the additional components with filtering and permeating functions on the basis of the existing closed container, the growth of microalgae in the reactor can be met, and the recycling of nutrients such as N and P can be realized, thereby embodying the resource recycling concept;

[0021] (4)Harmless algae can be cultured in the closed container after the additional components are installed, high-density growth of algal cells is realized, when a certain concentration is reached, the allelopathy effect of the secondary metabolites released by the algae acts on the algal blooms in the surrounding water, and the growth inhibition of other algae is induced, thereby realizing the inhibition effect on the algal blooms outside the device;

[0022] (5)The modified high-density cell bioreactor can be applied to the fermentation process in the food processing field, the high-density cell bioreactor can provide more cell growth space, promote the proliferation of strains and enzyme production and other biological reaction processes, the additional components with filtering and permeating functions can help remove waste and impurities, keep the culture environment clean, improve the purity and yield of fermentation products, and thereby improve the production efficiency;

[0023] (6)The modified high-density cell bioreactor can be applied to the pharmaceutical production field, in the pharmaceutical production process, the high-density cell culture reactor can be used for large-scale cultivation of cells to produce pharmaceutical active substances or biological agents, and the additional components with filtering and permeating functions can assist in removing waste and adjusting the culture medium composition during cell culture, thereby maintaining the stability of the culture environment and helping to improve the yield and purity of the drugs.

[0024] The technical scheme of the present application will be further described in detail below with reference to the drawings and embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the device of the present application;

[0026] Figure 2 It is a schematic diagram of the permeation part structure in the device of the present application;

[0027] Figure 3 It is a schematic diagram of the filtration part structure in the device of the present application;

[0028] Figure 4 It is a schematic diagram of the fixation part structure in the device of the present application;

[0029] Figure 5To compare the mass transfer rates of different semipermeable membranes, (a) shows the change of nitrate nitrogen concentration over time under different membrane material conditions; (b) shows the average mass transfer rate of different membrane materials.

[0030] Figure 6 The growth of microalgae in the experimental and control groups under nitrogen-deficient culture conditions is shown in (a) and (b) respectively. (a) shows the growth curves of microalgae PCC6803 under membrane conditions (experimental group) and non-membrane conditions (control group); (b) shows the change of dry weight of microalgae PCC6803 over time under membrane conditions (experimental group) and non-membrane conditions (control group).

[0031] Figure Labels

[0032] 1. Infiltration section; 2. Filtration section; 3. Fixing section;

[0033] 11. Semi-permeable membrane fixing disc; 12. First connecting part; 13. External thread; 14. Semi-permeable membrane; 15. Spiral groove;

[0034] 21. Filter disc; 22. Second connecting part; 23. Internal thread; 24. Filter hole;

[0035] 31. Threaded hole. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the present utility model embodiments clearer, the present utility model embodiments will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model embodiments and are not intended to limit the present utility model embodiments. All other embodiments obtained by those skilled in the art based on the embodiments in this application without creative effort are within the scope of protection of this application. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout.

[0037] It should be noted that the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or server that includes a series of steps or units is not necessarily limited to those steps or units that are explicitly listed, but may include other steps or units that are not explicitly listed or that are inherent to such process, method, product, or device.

[0038] Similar labels and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0039] Example 1

[0040] A device for modifying a sealed container into a high-density cell bioreactor, the structure of which is as follows: Figures 1-4 As shown, it includes a permeation section 1, a filtration section 2, and a fixing section 3.

[0041] The permeation section 1 includes a semi-permeable membrane fixing disk 11 and a first connecting part 12. The first connecting part 12 is a hollow cylindrical shape and is an integral structure with the semi-permeable membrane fixing disk 11. The first connecting part 12 is provided with external threads 13 for connecting with the filter section 2 and the fixing part 3.

[0042] A semipermeable membrane 14 is fixed at the center of the inner ring of the semipermeable membrane fixing disk 11. Several spiral grooves 15 are opened on the side of the semipermeable membrane fixing disk 11 away from the first connecting part 12 to promote liquid disturbance and guide the cell movement to the vicinity of the semipermeable membrane 14, so that the cell can make full use of the nutrients exchanged from outside the device.

[0043] The semipermeable membrane 14 has micropores with a diameter of less than 1 μm, while cell sizes are typically in the micrometer range. The purpose of the semipermeable membrane 14 is to block cells from freely passing through. Generally, the diameter of microalgal cells can vary from several micrometers to tens of micrometers; the corresponding micropore size of the semipermeable membrane 14 should be smaller than the diameter of the smallest microalgal cell to ensure that cells are not drawn in; however, the pore size cannot be too small, as an excessively small pore size may limit the efficiency of gas and liquid exchange.

[0044] The filter section 2 includes a filter disc 21 and a second connecting section 22. The second connecting section 22 is a hollow cylindrical shape and is an integral structure with the filter disc 21. The second connecting section 22 is provided with an internal thread 23, which is engaged with the external thread 13 of the first connecting section 12.

[0045] Several filter holes 24 are opened on the filter disc 21. The diameter of the filter holes 24 is between 12mm and 45mm. The size can be set according to the needs of different environments. The goal is to prevent external pollutants from entering the high-density cell bioreactor without hindering the exchange of nutrients.

[0046] The fixing part 3 has a threaded hole 31 at its center that mates with the external thread 13 of the first connecting part 12. The fixing part 31 is threadedly connected to the first connecting part 12 of the penetration part 1 through the threaded hole 31. The outer edge cross-sectional shape of the fixing part 3 can be set to any polygon or circle.

[0047] The permeation section 1 and the filtration section 2 are made of bio-inert polymer materials such as polyethylene, polyvinyl chloride, acrylic resin, polytetrafluoroethylene and organosilicon polymers, and can be selected according to actual needs during preparation.

[0048] The device is assembled on the closed container, first, the permeation part 1 is placed in the closed container, the first connecting part 12 passes through the through hole on the closed container, the diameter of the through hole is not greater than the diameter of the semi-permeable membrane fixing disc 11, the fixing part 3 is screwed on the first connecting part 12, the permeation part 1 is fixed through the pressure between the fixing part 3, the closed container and the semi-permeable membrane fixing disc 11, finally, the filter part 2 is screwed on the first connecting part 12, and the assembly is completed.

[0049] In addition to being fixed by pressure, the fixing part 3 and the closed container can be further fixed by coating a waterproof adhesive.

[0050] The semi-permeable membrane fixing disc 11 of the permeation part 1 is arranged in the high-density cell bioreactor, the semi-permeable membrane 14 blocks the passage of cells on one hand, and can control the osmotic pressure and gas exchange of the liquid inside and outside the high-density cell bioreactor on the other hand, and can adjust the permeation and exchange of nutrients and metabolites and other substances inside and outside the high-density cell bioreactor.

[0051] The filter part 2 is located outside the closed container, and the filter disc 21 is arranged, which does not affect the communication between the reactor and the external liquid, and can prevent external pollutants from entering the high-density cell bioreactor, protect the purity of the culture environment, and prolong the service life of the permeation part.

[0052] The fixing part 3 fixes the device on the closed container, prevents the device from moving or tilting during stirring or vibration, effectively ensures the stability and reliability of the high-density cell bioreactor, and ensures the normal growth of cells in the high-density cell bioreactor.

[0053] The device can be arranged on the side, top or bottom of the closed container, and the device is installed on the interface between the closed container and the external liquid, and the filter part 2 is immersed or at least half immersed in the external liquid, so as to not affect the free exchange and utilization of organic and inorganic molecules between the high-density cell bioreactor and the external liquid.

[0054] In the use process of the device, the closed container is opened and inoculated with microalgae, and then the device is assembled, the semi-permeable membrane 14 of the permeation part 1 can retain the algal cells in the reactor, which is helpful for the enrichment and biomass recovery of algae. By using the selective permeability of the semi-permeable membrane 14, the nutrient salt can be quickly transmitted in the semi-permeable membrane 14, realizing the in-situ treatment of the eutrophic water body by the microalgae. When the concentration of algal cells reaches a certain value, the secondary metabolite-mediated allelopathy effect can effectively inhibit the external algal bloom, so as to realize the high-density enrichment culture of one or several types of cells in the device.

[0055] Test example

[0056] The specific content of the screening test of the semi-permeable membrane is as follows:

[0057] Nitrate nitrogen (NO3) was selected - NO3- was used as a model nutrient to measure the mass transfer rate of the membrane material. - -N is used instead. NO3 is measured in different membrane materials. - The mass transfer rate of -N was evaluated and compared using membrane materials such as RC (regenerated cellulose), UE008 (polyethersulfone), UE100 (polyethersulfone), and microfiltration membranes including hydrophobic MF045 (polyvinylidene fluoride), hydrophilic MF045 (polyvinylidene fluoride), and PTFE (polytetrafluoroethylene).

[0058] The results are as follows Figure 5 As shown, (a) represents the change of nitrate nitrogen concentration over time under different membrane material conditions; (b) represents the average mass transfer rate of different membrane materials.

[0059] like Figure 5 As shown in (a), it can be seen that NO3 under different membrane materials with the same pore size... - The mass transfer rate of NO3- varies. However, for the same membrane material, under different pore sizes, the mass transfer rate of NO3- varies. - The nitrate nitrogen transfer efficiency also varies. It can be clearly observed that the hydrophilic polyvinylidene fluoride membrane material exhibits good mass transfer performance over time, with the highest nitrate nitrogen concentration after 20 hours.

[0060] like Figure 5 As shown in (b), by calculating the average mass transfer rate of the membrane materials, it can be clearly determined that the hydrophilic polyvinylidene fluoride membrane material has the highest mass transfer performance, reaching 1356 mg / L / h / m. 2 .

[0061] In summary, the modified hydrophilic polyvinylidene fluoride membrane material exhibits significant effects.

[0062] Hydrophilic polyvinylidene fluoride (PVDF) membrane material was used for microalgae cultivation in a high-density bioreactor, maintaining the initial inoculation of Synechocystis sp. PCC6803 within the device. 730 = Approximately 0.2. Growth experiments of microalgae were designed under conditions with and without a semi-permeable membrane (closed container), with three replicates for each group. The experiments were conducted in artificially prepared culture media to better control experimental variables.

[0063] Nitrogen-deficient culture was conducted by reducing the amount of NaNO3 in the culture medium. Without a membrane, material exchange is impossible. With a membrane, the algae inside the apparatus can obtain nutrients from the external environment.

[0064] The results are as follows Figure 6As shown, (a) shows the growth curves of microalga PCC6803 under membrane conditions (experimental group) and non-membrane conditions (control group); (b) shows the change in dry weight of microalga PCC6803 over time under membrane conditions (experimental group) and non-membrane conditions (control group).

[0065] Depend on Figure 6 It can be seen that on the 5th day of cultivation, there was a significant turning point between the experimental group and the control group. After the nitrogen nutrients were depleted, the experimental group was able to obtain certain nutrients from the outside world because of the membrane, while the control group could not obtain nutrients from the outside world, and its growth was significantly inhibited.

[0066] Therefore, this utility model provides a device for modifying a sealed container into a high-density cell bioreactor. The modified high-density cell bioreactor has a simple structure, effectively reduces the connection between components, lowers the production and maintenance costs of the reactor, is easy to promote and use, and has high practicality.

[0067] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it. Although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solution of this utility model, and these modifications or equivalent substitutions cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of this utility model.

Claims

1. A device for retrofitting a closed container into a high density cell bioreactor comprising detachably connected permeation, filtration and fixation sections, characterized in that: The permeation part comprises a semipermeable membrane fixing disc and a first connecting part, the semipermeable membrane fixing disc is arranged in the closed container, and the first connecting part is connected with the fixing part and the filtering part outside the closed container through the through hole of the closed container.

2. A device for retrofitting a closed container as a high density cell bioreactor according to claim 1, characterized in that: The first connecting part is a hollow cylinder with external threads, and the semipermeable membrane is fixed at the center of the inner ring of the semipermeable membrane fixing disc; the pore size of the semipermeable membrane is less than 1 μm.

3. A device for retrofitting a closed container as a high density cell bioreactor according to claim 2, characterized in that: A plurality of spiral grooves are arranged on the side of the semipermeable membrane fixing disc away from the first connecting part.

4. A device for retrofitting a closed container as a high density cell bioreactor according to claim 3, wherein: The filtering part comprises a filtering disc and a second connecting part, the second connecting part is a hollow cylinder with internal threads, and the internal threads of the second connecting part are matched with the external threads of the first connecting part.

5. A device for retrofitting a closed container as a high density cell bioreactor according to claim 4, wherein: A plurality of filtering holes are arranged on the filtering disc, and the diameter of the filtering holes is 12 mm-45 mm.

6. A device for retrofitting a closed container as a high density cell bioreactor according to claim 5, wherein: A threaded hole matched with the external threads of the first connecting part is arranged at the center of the fixing part.

7. A device for retrofitting a closed container as a high density cell bioreactor according to claim 6, characterized in that: The device is located at the interface between the modified high-density cell bioreactor and the external liquid, and the permeation part is immersed or at least half-immersed in the external liquid.

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