High-alkalinity protease purification production device

Abstract

The utility model discloses a high-alkalinity protease purification production device, which relates to the technical field of protease purification and comprises a fixing frame and a plurality of ion exchange columns, and the inner wall of the fixing frame is fixedly connected with a support plate and a positioning plate; the first clamping plates and the second clamping plates are arranged in the fixing frame at equal intervals. According to the ion exchange column limiting device, the ion exchange column is clamped through the first clamping plate and the second clamping plate, and then the multiple pressing rods are clamped above the bottom end of the ion exchange column by pushing the pull rod, so that the ion exchange column is rapidly limited, overall limiting is more stable, meanwhile, friction to the exterior of the ion exchange column is avoided, and the service life of the ion exchange column is prolonged. Therefore, the influence on the observation effect on materials in the ion exchange column is avoided; the liquid inlet pipes at the top end of the ion exchange column are supported by the upward moving guide frame, so that the multiple liquid inlet pipes can be effectively separated, the conditions of winding and knotting among the multiple liquid inlet pipes are avoided, the influence on material transportation is prevented, and meanwhile, the mounting and dismounting processes are more convenient and faster.

Description

Technical Field

[0001] This utility model relates to the field of protease purification technology, specifically a high-alkaline protease purification and production device. Background Technology

[0002] The purification and production of high-alkaline proteases is a complex process involving multiple steps, including microbial fermentation, separation and purification, and drying. The purification process mainly includes the following steps: using a multi-stage filtration system, such as coarse filtration, medium filtration, fine filtration, and high-precision filtration, to remove impurities; further separating and purifying the protease using an ion exchange column; and purifying using an aqueous two-phase system, which can effectively improve enzyme purity.

[0003] Existing ion exchange equipment, through the arrangement of multiple ion exchange columns, can efficiently purify proteases. These columns are typically connected to an external liquid supply system, such as a peristaltic pump, reservoir, or other liquid delivery device, via feed tubes to deliver samples or buffer solutions into the columns. Some ion exchange devices utilize elastic clamps to secure the columns; however, during installation and disassembly, the elasticity of these clamps can cause the columns to slip and fall, resulting in damage. Furthermore, the significant friction between the columns and the clamps during installation leads to substantial wear and scratches, which can impair observation of the column's interior. Additionally, the feed tubes at the top of multiple columns can become entangled due to their elasticity, potentially affecting material delivery, and disassembly is laborious and inconvenient. Utility Model Content

[0004] The purpose of this invention is to provide a high-alkaline protease purification and production device to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, this utility model provides a high-alkaline protease purification and production device, comprising,

[0006] A fixed frame and multiple ion exchange columns, wherein a support plate and a positioning plate are fixedly connected to the inner wall of the fixed frame;

[0007] Multiple card plates 1 and card plates 2 are equidistantly arranged inside the fixed frame. Card plate 1 is fixedly connected to the inner wall of the fixed frame, and card plate 2 passes through the fixed frame and is slidably connected to it.

[0008] A pull rod is slidably connected to the outside of the fixed frame. Multiple pressure rods are equidistantly fixed to one side of the pull rod, and the pressure rods pass through the fixed frame and are slidably connected to it.

[0009] Multiple guide frames, which are slidably connected to the rear outer wall;

[0010] A limiting module is located on the rear side of the fixed frame, and the limiting module is used to limit the movement of multiple guide frames.

[0011] Furthermore, a plurality of limiting rods are fixedly connected to one side of the pull rod. The limiting rods pass through the fixing frame and are slidably connected thereto. A locking block is slidably connected inside the positioning plate. The locking block can be movably engaged with the adjacent limiting rod. A return spring is fixedly connected between the locking block and the positioning plate.

[0012] Furthermore, a fixing rod is provided on the rear side of the fixing frame, and the bottom ends of the multiple second clamping plates are all fixedly connected to the fixing rod.

[0013] Furthermore, the top of the pull rod is provided with multiple slots, and the bottom of the fixing rod is fixedly connected with multiple mating plates, which can be movably engaged with adjacent slots.

[0014] Furthermore, a rubber skin layer is fixedly provided on the inner wall of both the first and second card plates.

[0015] Furthermore, the limiting module includes a limiting shell fixed to the rear outer wall of the fixing frame, a plurality of rectangular rods are slidably connected to the limiting shell, the bottom end of the guide frame is fixedly connected to an adjacent rectangular rod, a ratchet rack is fixedly connected to one side of the rectangular rod, a plurality of limiting blocks are equidistantly slidably connected inside the limiting shell, and a return spring is fixedly connected between the limiting block and the limiting shell.

[0016] Furthermore, an auxiliary plate is provided on the rear side of the limiting shell, and multiple limiting blocks pass through the auxiliary plate and are slidably connected to it.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows: When installing the ion exchange column, the bottom end of the ion exchange column is inserted into the positioning plate, and the ion exchange column is clamped by the first clamping plate and the second clamping plate. Then, by pushing the pull rod, multiple pressure rods are clamped above the bottom end of the ion exchange column, thereby quickly limiting the ion exchange column. The overall limiting is more stable, and it also avoids friction on the outside of the ion exchange column, thereby avoiding affecting the observation effect of the material inside the ion exchange column.

[0018] By using an upward-moving guide frame to support the inlet pipe at the top of the ion exchange column, multiple inlet pipes can be effectively separated, avoiding entanglement and knots between them, thus preventing any impact on material transportation. This also makes installation and disassembly more convenient. Attached Figure Description

[0019] Figure 1-2 This is a schematic diagram of the overall multi-angle structure of this utility model;

[0020] Figure 3 This is a side sectional view of the fixing frame structure in this utility model;

[0021] Figure 4 This is a side sectional view of the positioning plate in this utility model;

[0022] Figure 5 This is a side sectional view of the fixing rod structure in this utility model;

[0023] Figure 6 This is a schematic diagram of the limiting module structure in this utility model.

[0024] In the diagram: 10. Fixing frame; 11. Ion exchange column; 12. Support plate; 121. Positioning plate; 122. Locking block; 123. Reset spring one; 13. Locking plate one; 131. Locking plate two; 132. Fixing rod; 133. Connecting plate; 134. Rubber skin layer; 14. Pull rod; 141. Pressure rod; 142. Locking groove; 143. Limiting rod; 15. Guide frame; 16. Limiting module; 161. Limiting shell; 162. Rectangular rod; 163. Ratchet; 164. Limiting block; 165. Reset spring two; 166. Auxiliary plate. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Please see Figure 1-6 This utility model provides a technical solution: a high-alkaline protease purification and production device, comprising,

[0027] The fixture 10 and multiple ion exchange columns 11 are fixedly connected to the inner wall of the fixture 10, and a support plate 12 and a positioning plate 121 are fixedly connected to it.

[0028] Multiple card plates 13 and 131 are equidistantly arranged inside the fixed frame 10. Card plate 13 is fixedly connected to the inner wall of the fixed frame 10, and card plate 131 passes through the fixed frame 10 and is slidably connected to it.

[0029] A pull rod 14 is slidably connected to the outside of the fixed frame 10. Multiple pressure rods 141 are equidistantly fixed to one side of the pull rod 14. The pressure rods 141 pass through the fixed frame 10 and are slidably connected to it.

[0030] Multiple guide frames 15 are slidably connected to the rear outer wall;

[0031] The limiting module 16 is located on the rear side of the fixed frame 10 and is used to limit the movement of multiple guide frames 15.

[0032] In specific implementation, when installing the ion exchange column 11, the bottom end of the ion exchange column 11 is inserted into the positioning plate 121, and the support plate 12 is used for support to complete the initial positioning. The horizontal moving fixing rod 132 drives multiple clamping plates 131 to contact the ion exchange column 11. The ion exchange column 11 is clamped by the clamping plates 131 and 131. Then, by pushing the pull rod 14, multiple pressure rods 141 are clamped above the bottom end of the ion exchange column 11, thereby quickly positioning the ion exchange column 11. The overall positioning is more stable, and the ion exchange column 11 is not easy to fall off and cause damage during disassembly. At the same time, friction is avoided on the outside of the ion exchange column 11, thereby avoiding affecting the observation effect of the material inside the ion exchange column 11.

[0033] By supporting the liquid inlet pipe at the top of the ion exchange column 11 with the upward guide frame 15, multiple liquid inlet pipes can be effectively separated, avoiding entanglement and knotting between them, thus preventing any impact on material transportation. At the same time, it is more convenient during installation and disassembly.

[0034] See Figure 4 A plurality of limiting rods 143 are fixedly connected to one side of the pull rod 14. The limiting rods 143 pass through the fixing frame 10 and are slidably connected to it. A locking block 122 is slidably connected inside the positioning plate 121. The locking block 122 can be movably locked with the adjacent limiting rod 143. A return spring 123 is fixedly connected between the locking block 122 and the positioning plate 121.

[0035] In practice, the support plate 12 supports multiple ion exchange columns 11 and the positioning plate 121 provides equal-interval positioning for the multiple ion exchange columns 11. After the multiple ion exchange columns 11 are deployed, the pull rod 14 is moved to move multiple pressure rods 141 and bring them into contact with the ion exchange columns 11, placing the pressure rods 141 above the bottom of the ion exchange columns 11, thereby achieving initial positioning of the support plate 12. At this time, the ion exchange columns 11 can still rotate. During the movement of the pull rod 14, the limiting rod 143 moves the top arc surface of the locking block 122, causing the locking block 122 to move down. Under the reset action of the reset spring 123, the locking block 122 moves up and locks into the limiting rod 143, thereby quickly and automatically positioning the limiting rod 143 and the pull rod 14. When it is necessary to remove the multiple pressure rods 141, the locking block 122 is pulled down to remove them from the limiting rod 143. Then the pull rod 14 and the multiple pressure rods 141 can be adjusted.

[0036] See Figure 2-5 A fixing rod 132 is provided on the rear side of the fixing frame 10, and the bottom ends of multiple clamping plates 131 are fixedly connected to the fixing rod 132.

[0037] The top of the pull rod 14 is provided with multiple slots 142, and the bottom of the fixing rod 132 is fixedly connected with multiple docking plates 133, which can be movably engaged with adjacent slots 142.

[0038] Rubber skin 134 is fixedly installed on the inner wall of both the first card plate 13 and the second card plate 131.

[0039] In practice, the first clamp 13 and the second clamp 131 can clamp and limit the ion exchange column 11. The multiple second clamps 131 can be moved synchronously by the moving fixing rod 132, so as to facilitate the synchronous control of multiple ion exchange columns 11.

[0040] After the ion exchange column 11 is initially positioned inside the positioning plate 121, the first horizontal moving fixing rod 132 is activated, which drives multiple clamping plates 131 to contact the ion exchange column 11. The ion exchange column 11 is clamped by the clamping plates 131 and 131. Then, the pull rod 14 is pushed to contact the outer wall of the fixing frame 10, and multiple pressure rods 141 are clamped above the bottom of the ion exchange column 11. During the process, the docking plate 133 will be inserted into the slot 142, thereby automatically and quickly limiting the fixing rod 132 and multiple clamping plates 131, making the limiting of the ion exchange column 11 more convenient.

[0041] The rubber layer 134 facilitates increased friction between the first and second clamping plates 131 and the ion exchange column 11, limiting the upper part of the ion exchange column 11 while preventing the ion exchange column 11 from rotating on its own under slight impact, thereby enhancing the limiting effect on the ion exchange column 11.

[0042] See Figure 5-6 The limiting module 16 includes a limiting shell 161 fixed to the rear outer wall of the fixing frame 10. Multiple rectangular rods 162 are slidably connected to the limiting shell 161. The bottom end of the guide frame 15 is fixedly connected to the adjacent rectangular rod 162. A ratchet rack 163 is fixedly connected to one side of the rectangular rod 162. Multiple limiting blocks 164 are equidistantly slidably connected inside the limiting shell 161. A return spring 165 is fixedly connected between the limiting block 164 and the limiting shell 161.

[0043] An auxiliary plate 166 is provided on the rear side of the limiting shell 161, and multiple limiting blocks 164 pass through the auxiliary plate 166 and are slidably connected to it.

[0044] In practice, the rectangular rod 162 is limited by the limiting shell 161, thereby achieving vertical sliding limitation of multiple guide frames 15. The tube at the top of the ion exchange column 11 is called the feed tube or liquid inlet tube, and its main function is to introduce the liquid or resin to be treated into the column. The feed tube is usually connected to an external liquid supply system, such as a peristaltic pump, a storage tank, or other liquid delivery device. Since the liquid supply system is fixed in position, multiple ion exchange columns 11 are connected to the liquid supply system using feed tubes. Multiple feed tubes are easily mixed together under elastic action, which may affect the liquid inlet effect, and it is also difficult for operators to disassemble them. The feed tubes are moved upward by the guide frame 15 and the upper end of the guide frame 15 is used to guide the feed tube. The vertical support utilizes the space in the vertical direction, effectively separating multiple feed tubes, making the observation effect more intuitive and the subsequent disassembly more convenient. Since the distances between the multiple ion exchange columns 11 and the liquid supply equipment are different, the lengths of the feed tubes used are also different. When the guide frame 15 moves upward, it drives the rectangular rod 162 and the ratchet rack 163 to move upward. Under the limiting action of the limiting block 164, the limiting block 164 allows the guide frame 15 to move upward, but does not allow the guide frame 15 to move downward. After the guide frame 15 is released when it moves upward to the designated position, the guide frame 15 moves downward under the action of gravity, thereby automatically completing the limiting. It can be limited at any position within the movement range and can adapt to the limiting of feed tubes of different lengths.

[0045] By pulling the auxiliary plate 166, multiple limit blocks 164 can be moved synchronously. During the process, the second reset spring 165 is stretched, and multiple limit blocks 164 are separated from the ratchet rack 163 simultaneously, thereby releasing the limit on multiple guide frames 15, making disassembly more convenient.

[0046] Working principle: When installing the ion exchange column 11, the bottom end of the ion exchange column 11 is inserted into the positioning plate 121. The support plate 12 is used for support to complete the initial positioning. The horizontal moving fixing rod 132 drives multiple clamping plates 131 to contact the ion exchange column 11. The ion exchange column 11 is clamped by the clamping plates 131 and 131. Then, by pushing the pull rod 14, multiple pressure rods 141 are clamped above the bottom end of the ion exchange column 11, thereby quickly positioning the ion exchange column 11. The overall positioning is more stable, and the ion exchange column 11 is not easy to fall off and cause damage during disassembly. It also avoids friction on the outside of the ion exchange column 11, thereby avoiding affecting the observation effect of the material inside the ion exchange column 11.

[0047] By supporting the liquid inlet pipe at the top of the ion exchange column 11 with the upward guide frame 15, multiple liquid inlet pipes can be effectively separated, avoiding entanglement and knotting between them, thus preventing any impact on material transportation. At the same time, it is more convenient during installation and disassembly.

Claims

1. A high-alkaline protease purification and production apparatus, comprising, A mounting frame (10) and multiple ion exchange columns (11), wherein a support plate (12) and a positioning plate (121) are fixedly connected to the inner wall of the mounting frame (10); Multiple card plates one (13) and card plates two (131) are equidistantly arranged inside the fixed frame (10). The card plate one (13) is fixedly connected to the inner wall of the fixed frame (10), and the card plate two (131) passes through the fixed frame (10) and is slidably connected to it. A pull rod (14) is slidably connected to the outside of the fixed frame (10). A plurality of pressure rods (141) are fixedly connected at equal intervals on one side of the pull rod (14). The pressure rods (141) pass through the fixed frame (10) and are slidably connected to it. Multiple guide frames (15) are slidably connected to the rear outer wall; A limiting module (16) is provided on the rear side of the fixed frame (10), and the limiting module (16) is used to limit the multiple guide frames (15).

2. The high-alkaline protease purification and production apparatus as described in claim 1, characterized in that: A plurality of limiting rods (143) are fixedly connected to one side of the pull rod (14). The limiting rods (143) pass through the fixing frame (10) and are slidably connected thereto. A locking block (122) is slidably connected inside the positioning plate (121). The locking block (122) can be movably locked with the adjacent limiting rod (143). A return spring (123) is fixedly connected between the locking block (122) and the positioning plate (121).

3. The high-alkaline protease purification and production apparatus as described in claim 1, characterized in that: A fixing rod (132) is provided on the rear side of the fixing frame (10), and the bottom ends of the multiple card plates (131) are fixedly connected to the fixing rod (132).

4. The high-alkaline protease purification and production apparatus as described in claim 3, characterized in that: The top of the pull rod (14) is provided with multiple slots (142), and the bottom of the fixing rod (132) is fixedly connected with multiple docking plates (133). The docking plates (133) can be movably engaged with adjacent slots (142).

5. The high-alkaline protease purification and production apparatus as described in claim 1, characterized in that: A rubber skin layer (134) is fixedly provided on the inner wall of both the first card plate (13) and the second card plate (131).

6. The high-alkaline protease purification and production apparatus as described in claim 1, characterized in that: The limiting module (16) includes a limiting shell (161) fixed to the rear outer wall of the fixing frame (10). The limiting shell (161) is slidably connected to a plurality of rectangular rods (162). The bottom end of the guide frame (15) is fixedly connected to an adjacent rectangular rod (162). A ratchet rack (163) is fixedly connected to one side of the rectangular rod (162). A plurality of limiting blocks (164) are equidistantly slidably connected inside the limiting shell (161). A second return spring (165) is fixedly connected between the limiting block (164) and the limiting shell (161).

7. The high-alkaline protease purification and production apparatus as described in claim 6, characterized in that: An auxiliary plate (166) is provided on the rear side of the limiting shell (161), and multiple limiting blocks (164) pass through the auxiliary plate (166) and are slidably connected to it.

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