Magnetic bead separation and purification device

Abstract

The utility model relates to a magnetic bead separation and purification device which comprises a mixing container, a stirring assembly and a plurality of magnets. The magnetic field of the magnet can cover the interior of the mixing container to adsorb the magnetic beads, and can not cover the interior of the mixing container to enable the magnetic beads to move freely. Thus, the stirring head extends into the mixing container to stir liquid in the mixing container, and samples can be fully mixed in cooperation with the freely moving magnetic beads; in the waste liquid discharging process of the mixing container, the magnet can be used for adsorbing the magnetic beads, so that the magnetic beads are conveniently separated when the waste liquid is discharged; in the cleaning process, the stirring head can be used for stirring to accelerate magnetic bead cleaning, the magnet can also be used for adsorbing the magnetic beads, and magnetic bead separation is facilitated when purified products are discharged.

Description

Technical Field

[0001] This application relates to the field of separation and purification technology, and in particular to a magnetic bead separation and purification device. Background Technology

[0002] Traditional magnetic bead separation and purification methods typically involve placing the sample to be purified into a centrifuge tube and then placing it on a magnetic centrifuge tube holder for separation. However, in the scale-up and production stages of biopharmaceuticals, large-volume samples need to be processed. Conventional magnetic bead separation methods, due to the limited capacity of centrifuge tubes, require repeated operations to purify large volumes of samples, significantly increasing the workload and time required for separation and purification, resulting in low separation efficiency.

[0003] Chinese invention patent (application number CN201811510720.7) discloses a magnetic bead separation mechanism, device, and method. The device has a magnetic field generator set outside the container, preferably an electromagnet. The electromagnet is used to control the direction and magnitude of the magnetic field and to control the regular movement of the magnetic beads through the generated alternating magnetic field. However, when the container volume of the device is large, the following drawbacks exist: (1) The effective control distance between the electromagnet and the magnetic beads is limited. When the container volume is large, the electromagnet outside the container may not be able to effectively control the magnetic beads, resulting in insufficient contact between the magnetic beads and the sample and incomplete magnetic separation, thus affecting the separation effect; (2) In a large-volume container, the movement of the magnetic beads controlled by the alternating magnetic field is relatively small. Since the magnetic beads will settle due to gravity when the movement is small, it is not possible to completely avoid the aggregation of magnetic beads, resulting in insufficient contact between the magnetic beads and the sample, which affects the separation effect.

[0004] Chinese invention patent (application number CN202110322326.6) discloses a control method for a magnetic bead purification device. The device's magnetic components include alternating odd-numbered and even-numbered electromagnetic groups. The magnetic beads are controlled to move back and forth between these groups by alternating magnetic properties. These movements include continuous rotation, back-and-forth shaking, or up-and-down movement. While this device places the electromagnetic groups inside the container and improves the adsorption performance of the magnetic beads by controlling the spacing between the groups, thus solving the problem of limited control distance caused by placing the electromagnetic groups outside the container, when the container volume is large, the direction and manner of movement of the electromagnetic groups, which are parallel to the bottom plane of the container, cannot ensure a uniform distribution of the moving magnetic beads in the sample solution. This makes it difficult to guarantee consistent contact between the magnetic beads and the sample at each location, thus affecting the separation and purification effect. Utility Model Content

[0005] In view of this, the present application provides a magnetic bead separation and purification device to solve the problem of difficulty in fully separating and purifying large-volume samples in the prior art.

[0006] This application provides a magnetic bead separation and purification device, including:

[0007] A mixing container, the mixing container being hollow inside and capable of holding liquid, the mixing container having an inlet and an outlet at the bottom, and a cover plate at the top; the cover plate having an opening at the center, the opening communicating with the interior of the mixing container; and a number of slots arranged around the opening on the cover plate;

[0008] A stirring assembly includes a stirring head that can extend into the interior of the mixing container through the opening to stir the liquid inside the mixing container;

[0009] A plurality of magnets are arranged in a one-to-one correspondence with the slots, and each magnet is inserted into its corresponding slot. The magnets extend along the height direction of the mixing container. The magnetic field coverage of the magnets can be changed so that the magnetic beads in the mixing container are attracted or not attracted.

[0010] The inlet is used to obtain samples, cleaning solutions, or elution solutions from outside the mixing container, and the outlet is used to discharge the liquid inside the mixing container.

[0011] In an optional embodiment, the magnetic bead separation and purification device includes an inlet component and an outlet component. The inlet component is connected to the inlet via a pipe to supply the sample, washing solution, or elution solution to the mixing container.

[0012] The liquid outlet assembly is connected to the liquid outlet via a pipe to allow the purified product or waste liquid in the mixing container to be discharged and collected.

[0013] In an optional embodiment, the liquid inlet assembly includes: a sample container, a washing solution container, an elution solution container, and an inlet tube. The sample container is used to hold a sample, the washing solution container is used to hold a washing solution, and the elution solution container is used to hold an elution solution. The sample container, the washing solution container, and the elution solution container are connected to the inlet via the inlet tube.

[0014] In an optional embodiment, the inlet pipe includes a first switching valve and a first multi-way valve. The first switching valve is used to control the flow of liquid at the inlet, and the first multi-way valve is used to select one of the sample container, the cleaning solution container, and the elution solution container to be connected to the inlet.

[0015] In an optional embodiment, the liquid outlet assembly includes a product container, a waste liquid container, and a liquid outlet pipe. The product container is used to contain purified products, the waste liquid container is used to contain waste liquid, and the product container and the waste liquid container are connected to the liquid outlet via the liquid outlet pipe.

[0016] In an optional embodiment, the outlet pipe includes a second switching valve and a second multi-way valve. The second switching valve is used to control the flow of liquid at the outlet, and the second multi-way valve is used to select one of the product container and the waste container to be connected to the outlet.

[0017] In an optional embodiment, the magnet is a permanent magnet; the magnetic bead separation and purification device further includes a driving component for moving a plurality of the magnets to insert into or move away from the slot.

[0018] In an optional embodiment, the driving component includes:

[0019] A magnet support, wherein a magnet limiting groove is provided on the magnet support corresponding to the slot, and each magnet is installed in the corresponding magnet limiting groove;

[0020] A lifting mechanism, connected to the magnet support, is used to move the magnet support so that a plurality of the magnets can be moved to insert into or move away from the slot.

[0021] In an optional embodiment, the lifting mechanism includes:

[0022] Support platform;

[0023] A winding reel is rotatably mounted on the top of the support platform;

[0024] One end of the pull rope is fixed to the winding reel, and the other end is fixed to the top of the magnet bracket;

[0025] A drive motor is used to drive the winding wheel to rotate forward or backward, so that the winding wheel can retract or extend the pull rope.

[0026] In an optional embodiment, the cover plate includes a cover plate body and an isolation housing, the opening and the slot are formed in the cover plate body, and the isolation housing is arranged corresponding to the slot to isolate the magnet disposed in the slot from the liquid inside the mixing container.

[0027] In an optional embodiment, the stirring assembly further includes:

[0028] A connecting cover, the outline of which matches the opening, the connecting cover being able to be inserted into the opening to close it, and being able to be engaged and fixed with the cover plate; the connecting cover has an opening in the center;

[0029] A stirring drive is fixed on the connecting cover, and the drive shaft of the stirring drive passes through the opening and is connected to the stirring head.

[0030] In an optional embodiment, the magnet is an electromagnet, and the magnetic bead separation and purification device further includes a power supply component for energizing the magnet. The power supply component includes a switch for controlling whether to maintain or disconnect the power supply to the magnet.

[0031] The magnetic bead separation and purification device provided in this application includes a stirring assembly and several magnets. The magnetic field of the magnets can cover the inside of the mixing container to attract magnetic beads, or it can leave the inside of the mixing container uncovered to allow the magnetic beads to move freely. Thus, by inserting the stirring head into the mixing container to stir the liquid inside, the sample can be thoroughly mixed in conjunction with the freely moving magnetic beads. During the discharge of waste liquid from the mixing container, the magnets can be used to attract magnetic beads, facilitating their separation during waste liquid discharge. During the washing process, the stirring head can be used to accelerate the washing of magnetic beads, and the magnets can also be used to attract magnetic beads, facilitating their separation during the discharge of purified products.

[0032] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0033] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments of this application and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings:

[0034] Figure 1 This is a schematic diagram of the magnetic bead separation and purification device provided in Embodiment 1 of this application;

[0035] Figure 2 This is a schematic diagram of the external structure of the mixing container provided in Embodiment 1 of this application;

[0036] Figure 3 This is a schematic cross-sectional view of the mixing container provided in Embodiment 1 of this application;

[0037] Figure 4 This is a schematic diagram of the liquid inlet and outlet structure of the magnetic bead separation and purification device provided in Embodiment 1 of this application;

[0038] Figure 5 This is a schematic diagram showing the state of the magnet being inserted into the mixing container in the magnetic bead separation and purification device provided in Embodiment 1 of this application;

[0039] Figure 6 This is a partially enlarged view of the drive component structure provided in Embodiment 1 of this application;

[0040] Figure 7 This is a schematic diagram of the stirring assembly structure provided in Embodiment 1 of this application.

[0041] The attached figures are labeled as follows:

[0042] 1. Mixing container; 11. Inlet; 12. Outlet; 13. Cover; 131. Opening; 132. Slot; 133. Isolation shell;

[0043] 2. Stirring assembly; 21. Stirring head; 22. Connecting cover; 23. Stirring drive component; 221. Opening; 231. Drive shaft;

[0044] 3. Magnet;

[0045] 41. Sample container; 42. Washing solution container; 43. Elution solution container; 44. Inlet pipe; 441. First switching valve; 442. First multi-way valve;

[0046] 51. Product container; 52. Waste liquid container; 53. Discharge pipe; 531. Second switching valve; 532. Second multi-way valve;

[0047] 6. Drive assembly; 61. Magnet support; 62. Lifting mechanism; 611. Magnet limiting groove; 621. Support platform; 622. Winding wheel; 623. Pull rope; 624. Drive motor. Detailed Implementation

[0048] To make the technical solution and beneficial effects of this utility model more apparent and understandable, a detailed description is provided below by listing specific embodiments. The accompanying drawings are not necessarily drawn to scale, and local features may be enlarged or reduced to more clearly show the details of the local features; unless otherwise defined, the technical and scientific terms used herein have the same meanings as those in the technical field to which this application pertains.

[0049] In the description of this utility model, the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "height", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", and "counterclockwise" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the purpose of simplifying the description of this utility model and do not indicate that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. In other words, they should not be construed as limitations on this utility model.

[0050] In this utility model, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating the relative importance of the indicated features or the number of indicated technical features. Therefore, a feature specified as "first" or "second" can explicitly indicate that at least one of those features is included. In the description of this utility model, "multiple" means at least two, such as two, three, etc.; "several" means at least one, such as one, two, three, etc., unless otherwise explicitly specified.

[0051] In this utility model, unless otherwise explicitly defined, the terms "installation," "connection," "linking," "fixing," and "setting," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can also refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0052] In this utility model, unless otherwise explicitly defined, the terms "above," "on top of," "above," "over," "below," "below," "below," or "below" for "first feature above second feature" can refer to direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Furthermore, "above," "above," and "over" for "first feature above second feature" can mean the first feature is directly above or diagonally above the second feature, or simply indicates that the horizontal height of the first feature is higher than the horizontal height of the second feature. Similarly, "below," "below," and "below" for "first feature below second feature" can mean the first feature is directly below or diagonally below the second feature, or simply indicates that the horizontal height of the first feature is lower than the horizontal height of the second feature.

[0053] To fully understand this application, detailed steps and structures will be presented in the following description to illustrate the technical solution of this application. Preferred embodiments of this application are described in detail below; however, in addition to these detailed descriptions, this application may have other implementation methods.

[0054] Example 1

[0055] This embodiment provides a magnetic bead separation and purification device, such as... Figures 1-4 As shown, it includes: a mixing container 1, a stirring assembly 2, and several magnets 3.

[0056] The mixing container 1 is hollow inside and can hold liquid. The bottom of the mixing container 1 has an inlet 11 and an outlet 12, and the top has a cover 13. An opening 131 is formed in the center of the cover 13, connecting to the interior of the mixing container 1. Several slots 132 are arranged around the opening 131 on the cover 13. The interior of the mixing container 1 is used to hold liquid, the inlet 11 is used to obtain samples, washing solutions, or elution solutions from outside the mixing container 1, and the outlet 12 is used to discharge the liquid from inside the mixing container 1.

[0057] The stirring assembly 2 includes a stirring head 21, which extends into the mixing container 1 through an opening 131 to stir the liquid inside the mixing container 1. The stirring head 21 can significantly promote the movement of magnetic beads in the liquid, which is beneficial for the rapid and thorough mixing of the sample by the magnetic beads.

[0058] Several magnets 3 are arranged in a one-to-one correspondence with slots 132, and each magnet 3 is inserted into its corresponding slot 132. The magnets 3 extend along the height direction of the mixing container 1. The magnetic field coverage of the magnets 3 can be changed so that the magnetic beads in the mixing container 1 are attracted or not attracted. In this embodiment, the magnets 3 are permanent magnets, and the magnets 3 are movably inserted into the slots 132. The position of the movable magnets 3 can be changed to alter their magnetic field coverage.

[0059] The magnetic bead separation and purification device provided in this application includes a stirring assembly 2 and several magnets 3. The magnetic field of the magnets 3 can cover the inside of the mixing container 1 to adsorb magnetic beads, or it can leave the inside of the mixing container 1 uncovered to allow the magnetic beads to move freely. Thus, by extending the stirring head 21 into the mixing container 1, the liquid in the mixing container 1 is stirred, and the sample can be thoroughly mixed with the freely moving magnetic beads. During the discharge of waste liquid from the mixing container 1, the magnets 3 can be used to adsorb magnetic beads, facilitating the separation of magnetic beads when the waste liquid is discharged. During the washing process, the stirring head 21 can be used to stir and accelerate the washing of magnetic beads, and the magnets 3 can also be used to adsorb magnetic beads, facilitating the separation of magnetic beads when the purified product is discharged.

[0060] This embodiment provides a method for using the device, including the following steps:

[0061] S1, the sample is injected into the mixing container 1 through the liquid inlet 11, the magnet 3 is taken out from the corresponding slot 132, and the stirring component 2 is turned on to mix and adsorb the sample with the magnetic beads.

[0062] S2, turn off the stirring component 2, insert the magnets 3 into the corresponding slots 132 respectively to attract the magnetic beads, so that the magnetic beads are separated from the solution;

[0063] S3, the solution is discharged through the outlet 12;

[0064] S4, the cleaning solution is injected into the mixing container 1 through the liquid inlet 11, the magnet 3 is taken out from the corresponding slot 132, and the stirring component 2 is turned on so that the cleaning solution cleans the magnetic beads.

[0065] Repeat steps S2 and S3 above;

[0066] S5, the eluent is injected into the mixing container 1 through the liquid inlet 11, the magnet 3 is taken out from the corresponding slot 132, and the stirring assembly 2 is turned on so that the eluent washes off the adsorbate on the magnetic beads.

[0067] S6, after the purified product is discharged through the outlet 12, is collected.

[0068] In an alternative embodiment, such as Figure 4 As shown, the magnetic bead separation and purification device of this embodiment includes a liquid inlet component and a liquid outlet component. The liquid inlet component is connected to the liquid inlet 11 via a pipe to supply the mixing container 1 with samples, washing solution, or elution solution. The liquid outlet component is connected to the liquid outlet 12 via a pipe to discharge and collect the purified product or waste liquid in the mixing container 1. In this embodiment, the liquid inlet and liquid outlet components facilitate the introduction of the required liquid into the mixing container 1 at any time, and facilitate the removal of liquid from the mixing container 1 at any time.

[0069] In an alternative embodiment, such as Figure 4 As shown, the liquid inlet assembly includes a sample container 41, a washing solution container 42, an eluent container 43, and an inlet pipe 44. The sample container 41 is used to hold the sample, the washing solution container 42 is used to hold the washing solution, and the eluent container 43 is used to hold the eluent. The sample container 41, the washing solution container 42, and the eluent container 43 are connected to the inlet port 11 via the inlet pipe 44. In this embodiment, by separately setting the sample container 41, the washing solution container 42, and the eluent container 43, the individual supply of various required liquids can be achieved.

[0070] In an alternative embodiment, such as Figure 4 As shown, the inlet pipe 44 includes a first switching valve 441 and a first multi-way valve 442. The first switching valve 441 controls the flow of liquid at the inlet 11, and the first multi-way valve 442 selects one of the sample container 41, the washing solution container 42, or the eluent container 43 to be connected to the inlet 11. In this embodiment, the first switching valve 441 controls the flow of the main inlet pipe 44 connected to the inlet 11, and the first multi-way valve 442 enables selective supply of sample, washing solution, or eluent, simplifying the layout of the inlet pipe 44. The first switching valve 441 is only opened when sample, washing solution, or eluent is used; it needs to be closed at other times.

[0071] In an alternative embodiment, such as Figure 4As shown, the liquid discharge assembly includes a product container 51, a waste liquid container 52, and a liquid discharge pipe 53. The product container 51 is used to contain the purified product, and the waste liquid container 52 is used to contain the waste liquid. The product container 51 and the waste liquid container 52 are connected to the liquid outlet 12 through the liquid discharge pipe 53. In this embodiment, by separately setting the product container 51 and the waste liquid container 52, the waste liquid and the purified product are discharged independently.

[0072] In an alternative embodiment, such as Figure 4 As shown, the outlet pipe 53 includes a second switching valve 531 and a second multi-way valve 532. The second switching valve 531 controls the flow of liquid at the outlet 12, and the second multi-way valve 532 selects one of the product container 51 or the waste liquid container 52 to connect to the outlet 12. In this embodiment, the second switching valve 531 controls the flow of the main outlet pipe 53 connected to the outlet 12, and the second multi-way valve 532 enables selective discharge of waste liquid and purified product, simplifying the layout of the outlet pipe 53. The second switching valve 531 is only opened when discharging waste liquid or purified product and needs to be closed at other times.

[0073] In an alternative embodiment, such as Figure 1 , Figure 4 As shown, the magnetic bead separation and purification device of this embodiment further includes a driving component 6, which is used to move a plurality of magnets 3 to insert into or move away from the slot 132. In this embodiment, the driving component 6 facilitates the movement of the plurality of magnets 3.

[0074] In an alternative embodiment, such as Figure 1 , Figure 5 As shown, the driving component 6 includes a magnet support 61 and a lifting mechanism 62. The magnet support 61 has a magnet limiting groove 611 corresponding to the slot 132, and each magnet 3 is installed in the corresponding magnet limiting groove 611. The lifting mechanism 62 is connected to the magnet support 61 and is used to drive the magnet support 61 to move, so that several magnets 3 can move to insert into or move away from the slot 132. In this embodiment, the setting of the magnet support 61 facilitates the synchronous movement of several magnets 3, so that all magnets 3 can be inserted into or detached from the slot 132 simultaneously. The magnet limiting groove 611 corresponding to the slot 132 reduces the difficulty of inserting all magnets 3 into the slot 132.

[0075] In an alternative embodiment, such as Figure 1 , Figure 5 , Figure 6As shown, the lifting mechanism 62 includes: a support platform 621, a winding reel 622, a pull rope 623, and a drive motor 624. The winding reel 622 is rotatably mounted on the top of the support platform 621; one end of the pull rope 623 is fixed to the winding reel 622, and the other end is fixed to the top of the magnet bracket 61; the drive motor 624 is used to drive the winding reel 622 to rotate forward or reverse, so that the winding reel 622 can wind and unwind the pull rope 623. In this embodiment, the magnet bracket 61 is lifted and lowered by using the drive motor 624 and the winding reel 622 to pull the pull rope 623, that is, the drive motor 624 rotates forward to make the pull rope 623 wind around the winding reel 622, thereby driving the magnet bracket 61 to rise, as shown. Figure 1 As shown; and the drive motor 624 reverses to release the pull rope 623 from the winding wheel 622, thereby driving the magnet support 61 to descend, as... Figure 5 As shown in the figure. In this embodiment, the lifting mechanism 62 has a simple structure, low cost, and low operating difficulty, making it easy to put into practical use.

[0076] In an alternative embodiment, such as Figure 3 As shown, the cover plate 13 includes a cover plate body and an isolation shell 133. An opening 131 and a slot 132 are formed in the cover plate body. The isolation shell 133 is arranged corresponding to the slot 132 to isolate the magnet 3 installed in the slot 132 from the liquid inside the mixing container 1. In this embodiment, by providing the isolation shell 133 below the cover plate body, it is convenient to insert the magnet 3. The isolation shell 133 can isolate the inside of the mixing container 1 from the space where the magnet 3 is located, preventing external contamination from entering the mixing container 1 through the slot 132.

[0077] In an alternative embodiment, such as Figure 2 , Figure 7 As shown, the stirring assembly 2 also includes a connecting cover 22 and a stirring drive 23. The outline of the connecting cover 22 matches the opening 131, allowing the connecting cover 22 to be inserted into and closed within the opening 131, and to be engaged and fixed with the cover plate 13. The connecting cover 22 has an opening 221 at its center. The stirring drive 23 is fixed to the connecting cover 22, and the drive shaft 231 of the stirring drive 23 passes through the opening 221 and connects to the stirring head 21. In this embodiment, a stirring assembly 2 structure that can be quickly installed and disassembled is provided. Using this stirring assembly 2, it can be easily installed at the opening 131 of the cover plate 13. After being installed on the cover plate 13, the connecting cover 22 also serves to close and isolate the opening 131, preventing liquid from splashing out of the mixing container 1 during the stirring process.

[0078] In an optional embodiment, the stirring head 21 of the stirring assembly 2 can be arranged in several ways along the axial direction of the drive shaft 231, such as two, three or more. It is understood that when the height of the mixing container 1 is high and the capacity is large, more stirring heads 21 can be set as needed. The form of the stirring head 21 includes, but is not limited to, blade shape, rod shape, impeller, etc.

[0079] Example 2

[0080] This embodiment provides a magnetic bead separation and purification device, which differs from the magnetic bead separation and purification device provided in Embodiment 1 in that: in this embodiment, the magnet 3 is an electromagnet, and the magnetic bead separation and purification device also includes a power supply component, which is used to energize the magnet 3. The power supply component includes a switch, which is used to control whether to keep the power supply to the magnet 3 or disconnect the power supply.

[0081] In this embodiment, by using an electromagnet as the magnet 3, the magnetic field of the magnet 3 changes with the energization and de-energization, thus eliminating the need for the driving component 6 required in embodiment 1.

[0082] It should be understood that the above embodiments are exemplary and are not intended to encompass all possible implementations included in the claims. Various modifications and changes can be made to the above embodiments without departing from the scope of this disclosure. Similarly, the various technical features of the above embodiments can be arbitrarily combined to form other embodiments of this application that may not be explicitly described. Therefore, the above embodiments only illustrate several implementations of this application and do not limit the scope of protection of this patent application.

Claims

1. A magnetic bead separation and purification device, characterized in that, include: A mixing container (1) is hollow inside and can hold liquid. The bottom of the mixing container (1) has a liquid inlet (11) and a liquid outlet (12). The top of the mixing container (1) has a cover plate (13). The cover plate (13) has an opening (131) in the center, which communicates with the interior of the mixing container (1). Around the opening (131), a number of slots (132) are arranged on the cover plate (13). The stirring assembly (2) includes a stirring head (21) which can extend into the mixing container (1) through the opening (131) to stir the liquid in the mixing container (1); A plurality of magnets (3) are arranged in a one-to-one correspondence with the slots (132). The magnets (3) are respectively inserted into the corresponding slots (132). The magnets (3) extend along the height direction of the mixing container (1). The magnetic field coverage of the magnets (3) can be changed so that the magnetic beads in the mixing container (1) are attracted or not attracted. The inlet (11) is used to obtain samples, cleaning solutions or elution solutions from outside the mixing container (1), and the outlet (12) is used to discharge the liquid inside the mixing container (1).

2. The magnetic bead separation and purification device according to claim 1, characterized in that, The magnetic bead separation and purification device includes an inlet component and an outlet component. The inlet component is connected to the inlet (11) via a pipe to supply the sample, washing solution or elution solution to the mixing container (1). The liquid outlet assembly is connected to the liquid outlet (12) via a pipe to discharge and collect the purified product or waste liquid in the mixing container (1).

3. The magnetic bead separation and purification device according to claim 2, characterized in that, The liquid inlet assembly includes: a sample container (41), a cleaning solution container (42), an elution solution container (43), and an inlet tube (44). The sample container (41) is used to hold a sample, the cleaning solution container (42) is used to hold a cleaning solution, and the elution solution container (43) is used to hold an elution solution. The sample container (41), the cleaning solution container (42), and the elution solution container (43) are connected to the inlet port (11) through the inlet tube (44). The liquid inlet pipe (44) includes a first switching valve (441) and a first multi-way valve (442). The first switching valve (441) is used to control the flow of liquid at the liquid inlet (11). The first multi-way valve (442) is used to select one of the sample container (41), the cleaning fluid container (42) and the elution fluid container (43) to be connected to the liquid inlet (11).

4. The magnetic bead separation and purification device according to claim 2, characterized in that, The liquid outlet assembly includes: a product container (51), a waste liquid container (52), and a liquid outlet pipe (53). The product container (51) is used to contain the purified product, and the waste liquid container (52) is used to contain the waste liquid. The product container (51) and the waste liquid container (52) are connected to the liquid outlet (12) through the liquid outlet pipe (53). The liquid outlet pipe (53) includes a second switching valve (531) and a second multi-way valve (532). The second switching valve (531) is used to control the flow of liquid at the liquid outlet (12), and the second multi-way valve (532) is used to select one of the product container (51) and the waste liquid container (52) to be connected to the liquid outlet (12).

5. The magnetic bead separation and purification apparatus according to any one of claims 1-4, characterized in that, The magnet (3) is a permanent magnet; the magnetic bead separation and purification device further includes a drive component (6), which is used to drive a plurality of the magnets (3) to move to insert into the slot (132) or move away from the slot (132).

6. The magnetic bead separation and purification device according to claim 5, characterized in that, The driving component (6) includes: A magnet bracket (61) is provided with a magnet limiting groove (611) corresponding to the slot (132) on the magnet bracket (61), and each magnet (3) is installed in the corresponding magnet limiting groove (611); A lifting mechanism (62) is connected to the magnet support (61) and is used to move the magnet support (61) so that a plurality of magnets (3) can be moved to insert into or move away from the slot (132).

7. The magnetic bead separation and purification apparatus according to claim 6, characterized in that, The lifting mechanism (62) includes: Support platform (621); A winding reel (622) is rotatably mounted on top of the support platform (621); A pull rope (623) is fixed at one end to the winding wheel (622) and at the other end to the top of the magnet bracket (61); A drive unit (624) is used to drive the winding wheel (622) to rotate forward or reverse so that the winding wheel (622) can retract or extend the pull rope (623).

8. The magnetic bead separation and purification apparatus according to any one of claims 1-4, characterized in that, The cover plate (13) includes a cover plate body and an isolation shell (133). The opening (131) and the slot (132) are formed in the cover plate body. The isolation shell (133) is arranged corresponding to the slot (132) and is used to isolate the magnet (3) set in the slot (132) from the liquid inside the mixing container (1).

9. The magnetic bead separation and purification apparatus according to any one of claims 1-4, characterized in that, The stirring assembly (2) also includes: A connecting cover (22) is provided, the outline of which matches the opening (131). The connecting cover (22) can be inserted into the opening (131) to close it, and can be engaged and fixed with the cover plate (13). The connecting cover (22) has an opening (221) in the center. A stirring drive (23) is fixed on the connecting cover (22), and the drive shaft (231) of the stirring drive (23) passes through the opening (221) and is connected to the stirring head (21).

10. The magnetic bead separation and purification apparatus according to any one of claims 1-4, characterized in that, The magnet (3) is an electromagnet. The magnetic bead separation and purification device also includes a power supply component. The power supply component is used to energize the magnet (3). The power supply component includes a switch, which is used to control whether to keep the magnet (3) powered or disconnect the power supply.

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