Rotary constant-temperature tissue dissociation instrument

Abstract

The utility model discloses a rotary constant-temperature tissue dissociation instrument which comprises a constant-temperature device for placing a test tube, a rotating device and a control assembly, the control assembly drives the constant-temperature device to rotate through the rotating device, and the constant-temperature device comprises a constant-temperature bin and a clamping device arranged in the constant-temperature bin; the clamping device comprises two test tube clamping blocks which are oppositely and elastically arranged, the two test tube clamping blocks enclose to form an insertion hole for placing a test tube, and the constant-temperature bin is provided with a placement hole matched with the insertion hole for use; the two test tube clamping blocks have an abutting state or a separating state; when the two test tube clamping blocks abut against each other, the inner diameter of the insertion hole is smaller than that of the placement hole; when the test tube clamping blocks are far away from each other, the test tube is located in the insertion hole, and the inner diameter of the insertion hole is larger than that of the placement hole, according to the tissue dissociation instrument, the stability during rotation is guaranteed through clamping and fixing of the test tube, and the heating blocks are arranged on the outer walls of the test tube clamping blocks so that the tube wall of the test tube can be heated at a constant temperature, and the activity of tissue in the test tube can be improved.

Description

Technical Field

[0001] This utility model relates to the field of tissue dissociation instrument technology, specifically to a rotary constant temperature tissue dissociation instrument. Background Technology

[0002] A tissue dissociation device is a device that uses tissue dissociation technology to dissociate biological tissues to obtain highly active, high-quality cell suspensions. To obtain a cell suspension, the tissue dissociation device first needs to disrupt the extracellular matrix to release cells from it, and then break the connections between cells to obtain the cell suspension. There are two main methods of dispersion: one is to physically crush the tissue directly. This method has low cell dispersion efficiency and may cause some mechanical damage to the cells during the operation, resulting in a small number of usable cells. The other method is to treat the tissue blocks with enzymes or other reagents, and then mechanically disperse them into single cells. This method is relatively gentler, but it is more time-consuming and requires a lot of manual operation.

[0003] The existing tissue dissociation apparatus with publication number CN117327564A can only clamp and fix a certain section of the test tube during use, and the heating block is located at the bottom of the test tube. This setting cannot guarantee the stability of the test tube during rotation and the constant temperature heating efficiency is too slow, which reduces the quality of cell dissociation by the device and results in poor performance.

[0004] Therefore, there is an urgent need to provide a rotary isothermal tissue dissociation apparatus to solve the aforementioned technical problems. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a rotary isothermal tissue dissociation instrument. This tissue dissociation instrument not only ensures the stability of the test tube during rotation by clamping and fixing the test tube, but also improves the activity of the tissue inside the test tube by setting a heating block on the outer wall of the test tube clamp to keep the test tube wall at a constant temperature.

[0006] The technical solution of this utility model is: a rotary constant temperature tissue dissociation instrument, including a constant temperature device for placing test tubes, a rotating device and a control component. The control component drives the constant temperature device to rotate through the rotating device. The constant temperature device includes a constant temperature chamber and a clamping device disposed in the constant temperature chamber.

[0007] The clamping device includes two test tube clamping blocks that are relatively elastically arranged. The two test tube clamping blocks surround and form an insertion hole for placing test tubes. The constant temperature chamber is provided with a placement hole that is used in conjunction with the insertion hole.

[0008] The two test tube clamps can be in two states: either in contact or separated.

[0009] When the two test tube clamps abut against each other, the inner diameter of the insertion hole is smaller than the inner diameter of the placement hole;

[0010] When the test tube clamps are far apart, the test tube is located inside the insertion hole, and the inner diameter of the insertion hole is larger than the inner diameter of the placement hole.

[0011] Furthermore, the outer wall of the test tube clamp is provided with a heating block along the height direction.

[0012] Furthermore, the height of the heating block is less than the height of the test tube clamp block.

[0013] Furthermore, the constant temperature chamber is also equipped with two limiting seats. One end of the test tube clamp is elastically connected to the top of the constant temperature chamber, and the other end of the test tube clamp is elastically connected to the limiting seat.

[0014] The top of both the limiting seat and the constant temperature chamber is provided with a guide groove, and one end and the other end of the test tube clamp block move elastically back and forth within the guide groove.

[0015] Furthermore, the inner wall of the constant temperature chamber is provided with limiting reinforcing ribs, and the limiting seat is symmetrically fixed inside the constant temperature chamber by the limiting reinforcing ribs.

[0016] Furthermore, the bottom of the limiting seat is provided with a fixing seat.

[0017] Furthermore, the clamping assembly is provided in multiple sets.

[0018] Furthermore, the constant temperature chamber includes a cover and a bottom plate.

[0019] The beneficial technical effects of this utility model are:

[0020] 1. When the two test tube clamps are brought together, the inner diameter of the insertion hole formed by the clamps is smaller than the inner diameter of the placement hole, and the test tube is not placed in the insertion hole. When the two test tube clamps are moved away from each other, the inner diameter of the insertion hole formed by the clamps is larger than the inner diameter of the placement hole, and the test tube is placed in the insertion hole.

[0021] The elastic design of the test tube clamp not only facilitates the clamping and fixing of the test tube, ensuring its stability during rotation, but also makes it easy to pick up the test tube, freeing up the operator's hands.

[0022] 2. Each test tube clamp is equipped with a heating block on its outer wall. The heating block transfers heat from the test tube clamp to the test tube wall to keep the test tube at a constant temperature, thereby improving heating efficiency and ensuring the activity of the tissue inside the test tube.

[0023] 3. Since the limiting seats are set opposite each other and the two limiting seats are not a whole, the two limiting seats are used for two test tube clamps respectively. The two limiting seats are fixed by the fixing seat, which further ensures the smooth movement of the test tube clamps.

[0024] 4. The test tube clamp can hold and fix most of the test tube, which improves the stability of the test tube compared to the existing technology that only fixes a certain part of the test tube. At the same time, heating blocks are placed at the fixed positions of the test tube clamp. It can be understood that the heating blocks can keep the test tube heated at a constant temperature according to the size of the test tube covered by the test tube clamp.

[0025] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0027] Figure 2 This is a schematic diagram of the structure of the constant temperature device of this utility model;

[0028] Figure 3 This is a schematic diagram of the split structure of the constant temperature chamber of this utility model;

[0029] Figure 4 This is a schematic diagram of the clamping assembly of this utility model;

[0030] Figure 5 This is a schematic diagram of the connection between the limiting seat and the constant temperature chamber of this utility model.

[0031] The attached figures are labeled as follows:

[0032] 100. Control component; 200. Rotation device; 300. Temperature control device; 310. Temperature control chamber; 311. Placement hole; 312. Chamber cover; 3121. Limiting reinforcing rib; 313. Base plate; 314. Guide groove; 320. Clamping device; 321. Test tube clamping block; 3211. Spring; 322. Insertion hole; 323. Limiting seat; 324. Fixing seat; 330. Heating block. Detailed Implementation

[0033] In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0034] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate for the embodiments of this application described herein.

[0035] In the description of this utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship described in the embodiments and shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0036] like Figure 1 As shown, this utility model specifically relates to a rotary thermostatic tissue dissociation apparatus, including a thermostatic device 300 for placing test tubes, a rotating device 200, and a control component 100. The control component 100 drives the thermostatic device 300 to rotate through the rotating device 200. The thermostatic device 300 includes a thermostatic chamber 310 and a clamping device 320 disposed in the thermostatic chamber 310.

[0037] The clamping device 320 includes two test tube clamping blocks 321 that are relatively elastically arranged. The two test tube clamping blocks 321 surround and form an insertion hole 322 for placing test tubes. The constant temperature chamber 310 is provided with a placement hole 311 that is used in conjunction with the insertion hole 322.

[0038] The two test tube clamps 321 can be in two states: either in contact or separated.

[0039] When the two test tube clamps 321 abut against each other, the inner diameter of the insertion hole 322 is smaller than the inner diameter of the placement hole 311;

[0040] When the test tube clamps 321 are far apart, the test tube is located inside the insertion hole 322, and the inner diameter of the insertion hole 322 is larger than the inner diameter of the placement hole 311.

[0041] It should be noted that the prior art with publication number CN117327564A has already disclosed in detail the relationship between the constant temperature device 300, the rotating device 200 and the control component 100, as well as how the control component 100 drives the constant temperature device 300 to rotate through the rotating device 200, and will not be described in detail here.

[0042] The two test tube clamps 321 are elastically set within the constant temperature chamber 310, forming an insertion hole 322 for placing test tubes. Similarly, the constant temperature chamber 310 is provided with a placement hole 311 that matches the insertion hole 322. Since the two test tube clamps 321 are elastically set within the constant temperature chamber 310, when there is no external force, that is, when no test tubes are placed, the elasticity causes the two test tube clamps 321 to abut against each other. At this time, the inner diameter of the insertion hole 322 between the two test tube clamps 321 is smaller than the inner diameter of the placement hole 311.

[0043] When the test tube is inserted between the two test tube clamps 321 through the placement hole 311, the test tube abuts against the two test tube clamps 321, causing them to move in opposite directions and eventually separate. When the test tube reaches the designated position within the constant temperature chamber 310, the position of the two test tube clamps 321 stops changing, and the test tube is fixed in place by elastic force to ensure its stability. At this time, the inner diameter of the insertion hole 322 between the two test tube clamps 321 is larger than the inner diameter of the placement hole 311.

[0044] The test tube clamp 321 covers the portion of the test tube located inside the constant temperature chamber 310, which is more stable than the prior art that only fixes a certain section of the test tube.

[0045] The outer wall of the test tube clamp 321 is provided with heating blocks 330 along the height direction. Each test tube clamp 321 is equipped with one heating block 330. A test tube is fixed by two test tube clamps 321 and is also equipped with two heating blocks 330. The test tube wall is heated at a constant temperature from all directions by the two heating blocks 330.

[0046] The height of the heating block 330 is less than the height of the test tube clamp block 321. The area covered by the heating block 330 is increased, thereby increasing the range of heatable test tubes and improving heating efficiency.

[0047] The constant temperature chamber 310 is also provided with two limiting seats 323. One end of the test tube clamp 321 is elastically connected to the top of the constant temperature chamber 310, and the other end of the test tube clamp 321 is elastically connected to the limiting seat 323.

[0048] The top of both the limiting seat 323 and the constant temperature chamber 310 is provided with a guide groove 314, and one end and the other end of the test tube clamp 321 move elastically back and forth in the guide groove 314 respectively.

[0049] One end of the test tube clamp 321 abuts against the top of the constant temperature chamber 310, and the other end of the test tube clamp 321 abuts against the limiting seat 323. Similarly, springs 3211 are provided on the same side of both ends of the test tube clamp 321. The springs 3211 are connected to the constant temperature chamber 310 and the limiting seat 323 respectively. When the test tube clamp 321 receives an external force, the test tube clamp 321 moves along the direction of the guide groove 314. Similarly, when the external force disappears, the elasticity of the spring 3211 causes the test tube clamp 321 to move along the direction of the guide groove 314 to restore its original position.

[0050] When the test tube is inserted between the two test tube clamps 321, the two test tube clamps 321 convert the vertical force into a horizontal force, causing the two test tube clamps 321 to move laterally in opposite directions.

[0051] The inner wall of the constant temperature chamber 310 is provided with limiting reinforcing ribs 3121, and the limiting seat 323 is symmetrically fixed inside the constant temperature chamber 310 by the limiting reinforcing ribs 3121.

[0052] The position of the limiting seat 323 is fixed by the limiting reinforcing rib 3121, and then the position of the test tube clamp 321 is fixed by the limiting seat 323, thus ensuring the overall stability during operation.

[0053] The bottom of the limiting seat 323 is provided with a fixing seat 324. Since the limiting seats 323 are arranged opposite each other and the two limiting seats 323 are not a whole, the two limiting seats 323 are used for the two test tube clamps 321 respectively. The fixing seat 324 is used to fix the two limiting seats 323, which further ensures the smooth movement of the test tube clamps 321.

[0054] The clamping assembly is provided in multiple sets, which can simultaneously dissociate tissues from multiple test tubes.

[0055] The constant temperature chamber 310 includes a chamber cover 312 and a bottom plate 313.

[0056] The above embodiments are merely specific implementations of this utility model, used to illustrate the technical solutions of this utility model, and not to limit it. The protection scope of this utility model is not limited thereto. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features within the technical scope disclosed in this utility model. Such modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model, and should all be covered within the protection scope of this utility model.

Claims

1. A rotary thermostatic tissue dissociation apparatus, comprising a thermostatic device (300) for holding test tubes, a rotating device (200), and a control component (100), wherein the control component (100) drives the thermostatic device (300) to rotate via the rotating device (200), characterized in that, The constant temperature device (300) includes a constant temperature chamber (310) and a clamping device (320) disposed in the constant temperature chamber (310); The clamping device (320) includes two test tube clamping blocks (321) that are relatively elastically arranged. The two test tube clamping blocks (321) surround each other to form an insertion hole (322) for placing test tubes. The constant temperature chamber (310) is provided with a placement hole (311) that is used in conjunction with the insertion hole (322). The two test tube clamps (321) can be in two states: either in contact or separated. When the two test tube clamps (321) abut against each other, the inner diameter of the insertion hole (322) is smaller than the inner diameter of the placement hole (311); When the test tube clamps (321) are far apart, the test tube is located in the insertion hole (322), and the inner diameter of the insertion hole (322) is greater than the inner diameter of the placement hole (311).

2. The rotary isothermal tissue dissociation apparatus according to claim 1, characterized in that, The outer wall of the test tube clamp (321) is provided with a heating block (330) along the height direction.

3. The rotary isothermal tissue dissociation apparatus according to claim 2, characterized in that, The height of the heating block (330) is less than the height of the test tube clamp block (321).

4. The rotary isothermal tissue dissociation apparatus according to claim 1, characterized in that, The constant temperature chamber (310) is also provided with two limiting seats (323). One end of the test tube clamp (321) is elastically connected to the top of the constant temperature chamber (310), and the other end of the test tube clamp (321) is elastically connected to the limiting seat (323). The top of both the limiting seat (323) and the constant temperature chamber (310) is provided with a guide groove (314), and one end and the other end of the test tube clamp (321) move elastically back and forth in the guide groove (314).

5. The rotary isothermal tissue dissociation apparatus according to claim 4, characterized in that, The inner wall of the constant temperature chamber (310) is provided with limiting reinforcing ribs (3121), and the limiting seat (323) is symmetrically fixed in the constant temperature chamber (310) by the limiting reinforcing ribs (3121).

6. The rotary isothermal tissue dissociation apparatus according to claim 5, characterized in that, The bottom of the limiting seat (323) is provided with a fixing seat (324).

7. The rotary isothermal tissue dissociation apparatus according to claim 1, characterized in that, The clamping device is provided in multiple sets.

8. The rotary isothermal tissue dissociation apparatus according to claim 1, characterized in that, The constant temperature chamber (310) includes a cover (312) and a bottom plate (313).

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