Cooling device for protein electrophoresis

Abstract

The utility model discloses a cooling device for protein electrophoresis, which comprises a cooling tank, cavities are arranged on the side wall and the bottom of the cooling tank, cooling materials are filled in the cavities, and a visible groove is arranged on the front side of the cooling tank. When protein electrophoresis is carried out and electrophoresis is carried out, a protein electrophoresis apparatus is placed in the cooling device, so that the temperature of a buffer solution in the protein electrophoresis apparatus is reduced in the electrophoresis process, and the influence of a heat effect on electrophoresis is reduced. Besides, the cooling device for protein electrophoresis is further provided with the visual groove, and the visual groove is designed to remarkably improve the visibility of a loading sample (such as SDS-PAGE loading) after the protein electrophoresis apparatus is arranged in the cooling device. Through the visual angle provided by the visual groove, a user can clearly observe the sample loading condition and the glue leakage state of the sample, so that the operation accuracy is ensured.

Description

Technical Field

[0001] This utility model relates to the field of experimental auxiliary equipment technology, and in particular to a cooling device for protein electrophoresis. Background Technology

[0002] Protein electrophoresis is a technique that uses an electric field to cause protein molecules to move along an electrophoretic medium. The principle of protein separation by electrophoresis is based on the differences in protein charge, i.e., their acid-base properties. During electrophoresis, proteins maintain their integrity and gradually separate according to their molecular weight, shape, and the amount of charge they carry. Electrophoresis apparatus generates heat during operation due to Joule heating caused by ion migration under the electric field. Joule heating can increase the temperature of the buffer solution, decrease the viscosity of the medium, intensify molecular motion, and reduce resolution. Furthermore, excessive Joule heating can burn the filter paper and char the polyacrylamide gel support medium, potentially disrupting the protein electrophoresis process and ultimately affecting the results. To ensure successful electrophoresis, especially under high-voltage electrophoresis, it is necessary to minimize the impact of heat on the electrophoresis process.

[0003] Therefore, existing technologies still need to be improved and developed. Utility Model Content

[0004] In view of the shortcomings of the prior art, the present invention provides a cooling device for protein electrophoresis, thereby solving the problem that the heat generated by the electrophoresis apparatus affects the electrophoresis effect during the protein electrophoresis process.

[0005] The technical solution adopted by this utility model to solve the above-mentioned technical problems is as follows:

[0006] In a first aspect, this utility model provides a cooling device for protein electrophoresis, the cooling device for protein electrophoresis including a cooling tank, the side wall of the cooling tank having a cavity, the cavity being filled with a cooling material, and a visible groove being formed on the front of the cooling tank.

[0007] Preferably, there are two visible grooves, which are arranged symmetrically.

[0008] Preferably, the height ratio of the visible groove to the height of the cooling groove is 2:5.

[0009] Preferably, the bottom wall of the cooling tank is also provided with a cavity.

[0010] Preferably, the side wall of the cooling tank is provided with an inlet for cooling material and water.

[0011] Preferably, the edges of the cooling groove are arc-shaped.

[0012] Preferably, the inner wall of the cooling tank is provided with a fixing groove.

[0013] Preferably, multiple fixing slots are provided.

[0014] Beneficial effects:

[0015] This utility model discloses a cooling device for protein electrophoresis. During protein electrophoresis, the protein electrophoresis apparatus is placed within this cooling device, which lowers the temperature of the buffer solution inside the apparatus, reducing the impact of heat on the electrophoresis process. Furthermore, the cooling device also features a viewing groove. This groove significantly improves the visibility of loaded samples (such as SDS-PAGE samples) after the protein electrophoresis apparatus is placed inside the cooling device. The viewing angle provided by the groove allows users to clearly observe the sample loading process and the gel running status, ensuring operational accuracy. Attached Figure Description

[0016] Figure 1 This is a front view of the structural schematic diagram of the cooling device for protein electrophoresis provided in this embodiment of the utility model;

[0017] Figure 2 This is the left view in the structural schematic diagram of the cooling device for protein electrophoresis provided in this embodiment of the utility model;

[0018] Figure 3 This is a top view of the structural schematic diagram of the cooling device for protein electrophoresis provided in this embodiment of the utility model;

[0019] Figure 4 This is a schematic diagram of the overall structure of the cooling device for protein electrophoresis provided in this embodiment of the utility model;

[0020] Figure 5 This is a front view of a schematic diagram showing the combination of a cooling device for protein electrophoresis and a protein electrophoresis apparatus provided in this embodiment of the present invention.

[0021] Figure 6 This is the left view in the schematic diagram of the combination of the cooling device for protein electrophoresis and the protein electrophoresis apparatus provided in this embodiment of the utility model;

[0022] Figure 7 This is a top view of a schematic diagram showing the combination of a cooling device for protein electrophoresis and a protein electrophoresis apparatus provided in an embodiment of this utility model.

[0023] Figure 8 This is a schematic diagram of the overall combination of the cooling device for protein electrophoresis and the protein electrophoresis apparatus provided in this embodiment of the utility model;

[0024] The figures are labeled as follows: 1. Cooling tank; 11. Visible groove; 12. Inlet; 13. Fixing groove. Detailed Implementation

[0025] This invention provides a cooling device for protein electrophoresis. To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the following provides a more detailed description of this invention. It should be understood that the specific embodiments described herein are merely illustrative of this invention and are not intended to limit this invention.

[0026] This utility model provides a cooling device for protein electrophoresis, the structural schematic diagram of which is shown below. Figure 1-4 ( Figure 1-4 (The front view, left view, top view and overall structural diagram are shown in sequence). The cooling device for protein electrophoresis includes a cooling tank 1. The side wall of the cooling tank 1 is provided with a cavity, which is filled with cooling material. A visible groove 11 is provided on the front of the cooling tank 1.

[0027] During protein electrophoresis, the protein electrophoresis apparatus is placed in the cooling device of this application, see [link to application]. Figure 5-8 ( Figure 5-8 (The images are shown in sequence: front view, left view, top view, and overall combined schematic diagram). This design lowers the temperature of the buffer solution within the protein electrophoresis apparatus during electrophoresis, reducing the impact of thermal effects on electrophoresis. Furthermore, the cooling device for protein electrophoresis in this application includes a viewing groove 11. This groove significantly improves the visibility of loaded samples (such as SDS-PAGE samples) after the protein electrophoresis apparatus is installed in the cooling device. Through the viewing angle provided by the groove 11, users can clearly observe the sample loading process and gel running status, ensuring operational accuracy. This design greatly reduces errors caused by limited viewing angles, improving experimental efficiency and reliability.

[0028] In some embodiments, there are two visible grooves 11, which are arranged symmetrically.

[0029] In some embodiments, the height ratio of the visible groove 11 to the height of the cooling groove 1 is 2:5.

[0030] In some embodiments, the bottom wall of the cooling tank 1 is also provided with a cavity.

[0031] A cavity is also provided on the bottom wall of cooling tank 1 to further increase the cooling area and ensure the cooling effect.

[0032] In some embodiments, the sidewall of the cooling tank 1 is provided with an inlet 12 for cooling material and water.

[0033] The preferred cooling material in this embodiment is sodium polyacrylate, a highly absorbent resin capable of absorbing hundreds of times its own weight in water. When mixed with water, sodium polyacrylate forms a gel upon hydration. This gel, after freezing, can prolong the thawing time (10-14 hours) and maintain stable properties, making it suitable as a heat exchange medium. Sodium polyacrylate has a large cold capacity, is non-toxic, odorless, non-corrosive, non-radioactive, and elastic. It is a novel freezing medium that does not cause water pollution during thawing and can be reused repeatedly. Its effective cold capacity is 3-4 times that of ice, making it a suitable replacement for ice as a heat exchange carrier for transferring heat.

[0034] When using this cooling device, add sodium polyacrylate powder through inlet 12 of cooling tank 1, then add an appropriate amount of water and mix. After mixing, freeze at -20°C overnight. After freezing, remove the cooling device, place the protein electrophoresis apparatus into the cooling device, and perform sample loading and electrophoresis. After electrophoresis, the cooling device can be returned to the -20°C freezer for continuous reuse. The operation is simple.

[0035] In some embodiments, the edges of the cooling tank 1 are arc-shaped.

[0036] The edges of cooling tank 1 are rounded to reduce stress concentration, enhance the fatigue resistance of the cooling tank under high and low temperature changes, and prevent stress cracks. Furthermore, the rounded design is safer, reducing the risk of accidental injury from sharp edges.

[0037] In some embodiments, the inner wall of the cooling tank 1 is provided with a fixing groove 13.

[0038] The protein electrophoresis apparatus is placed in the cooling device of this application, and the protein electrophoresis apparatus is snapped into the cooling tank 1. The opening of the fixing tank 13 increases the stability of the protein electrophoresis apparatus in the cooling device of this application.

[0039] In some embodiments, the fixing groove 13 is provided with multiple grooves.

[0040] It should be understood that the application of this utility model is not limited to the examples above. Those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. A cooling device for protein electrophoresis, characterized in that, The cooling device for protein electrophoresis includes a cooling tank, the side wall of which is provided with a cavity, the cavity is filled with a cooling material, and a visible groove is provided on the front of the cooling tank. There are two visible grooves, which are arranged symmetrically. The ratio of the height of the visible recess to the height of the cooling groove is 2:5; The bottom wall of the cooling tank is also provided with a cavity; The cooling tank has inlets for cooling material and water on its side wall; The edges of the cooling groove are arc-shaped; The inner wall of the cooling tank is provided with a fixing groove.

2. The cooling device for protein electrophoresis according to claim 1, characterized in that, Multiple fixing slots are provided.

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