Ultrafiltration centrifuge tube

Abstract

The utility model provides an ultrafiltration centrifuge tube which comprises a tube body and a tube cover covering a port of the tube body, the tube body comprises a central tube and an outer tube sleeved outside the central tube, a first chamber is arranged in the central tube, a second chamber is formed by a gap between the outer wall of the central tube and the inner wall of the outer tube, and the first chamber is communicated with the second chamber. The central pipe comprises an annular part at the upper end and a filtering part at the lower end, a first filtering piece and a second filtering piece which are oppositely arranged are arranged on the filtering part, membranes are fixed to the inner sides of the first filtering piece and the second filtering piece in a welded mode, the first filtering piece and the filtering part are integrally formed, and the second filtering piece is fixed to the filtering part in a welded mode.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of ultrafiltration centrifugal tube, especially to an ultrafiltration centrifugal tube. BACKGROUND

[0002] Purifying macromolecular components in tissue culture medium extract, purifying macromolecular components in cell lysate, removing primers, connection or molecular markers from reaction mixture, and removing protein before HPLC; or, desalination, buffer replacement or diafiltration, etc. all need to use separation methods to complete.

[0003] Membrane separation is a very efficient separation method. In the field of life science, membrane separation gradually replaces traditional separation methods such as chemical precipitation method, dialysis method, freeze-drying method, etc. However, membrane separation has very strict requirements when forming components. Local sealing is not good, which will cause leakage, seriously affect the separation effect, and make the experimental data lose credibility. Among them, ultrafiltration is a membrane separation method that uses ultrafiltration membrane with certain retention capacity as filter medium and pressure difference as power to separate very small particles and soluble molecules in solution. Compared with traditional methods, ultrafiltration has the advantages of mildness, convenience, rapidness, high efficiency, etc. Isovolumetric ultrafiltration is very effective for sample desalination and buffer replacement.

[0004] The ultrafiltration centrifugal tube is generally composed of an outer tube, a bearing tube, a bearing sleeve, an ultrafiltration membrane and the like. The bearing tube, the bearing sleeve and the ultrafiltration membrane are assembled in the outer tube. The ultrafiltration membrane is arranged in close contact with the filter hole on the surface of the bearing tube. The bearing sleeve cooperates with the bearing tube to fix the ultrafiltration membrane. In use, the ultrafiltration centrifugal tube containing the filter stock solution is placed in the centrifuge for centrifugation. After the centrifuge is started, the filter stock solution is continuously supplemented into the bearing tube. In this way, the filter stock solution is separated by the ultrafiltration membrane under the action of the centrifuge and is thrown into the bottom cavity of the outer tube, thereby realizing membrane separation.

[0005] The ultrafiltration centrifugal tube has the function of rapid ultrafiltration, can achieve a high concentration coefficient, is easy to recover concentrated liquid from dilute liquid and complex sample combination, and is therefore widely used in the field of biological filtration. The ultrafiltration centrifugal tube is mainly used for concentrating biological samples containing antigens, antibodies, enzymes, nucleic acids (single or double DNA / RNA samples), microorganisms, eluate and purified samples.

[0006] However, the existing ultrafiltration centrifugal tube still has a series of problems.

[0007] The existing ultrafiltration centrifugal tube adopts silica gel sealing ring or adhesive to realize sealing with plastic parts, the sealing mode using silica gel ring is prone to leakage after being subjected to centrifugal force in use, the sealing mode using adhesive is prone to residual adhesive composition, which increases the dissolution and affects the final test result, and the curing time of the adhesive is long, so that the efficiency of sealing assembly is low, the existing modes have poor use experience and affect the separation effect and impurity content of the finished product. Utility model content

[0008] Therefore, it is necessary to provide an ultrafiltration centrifugal tube.

[0009] To solve the above technical problems, the utility model provides an ultrafiltration centrifugal tube, which comprises a tube body and a tube cover arranged at the port of the tube body, the tube body comprises a center tube and an outer tube sleeved outside the center tube, the center tube has a first chamber in the inside, and a second chamber is formed between the outer wall of the center tube and the inner wall of the outer tube, the center tube comprises an annular part at the upper end and a filter part at the lower end, the first filter sheet and the second filter sheet are oppositely arranged on the filter part, the inner side of the first filter sheet and the second filter sheet is fixed with a diaphragm through welding, the first filter sheet is integrally formed with the filter part, and the second filter sheet is fixed on the filter part through welding.

[0010] Preferably, the first filter sheet has an included angle with the vertical direction, and the included angle ranges from 1 to 10 degrees, and the second filter sheet is mirror-symmetric with the first filter sheet.

[0011] Preferably, the diaphragm is fixed with the first filter sheet and the second filter sheet through hot welding, and the second filter sheet is fixed on the center tube through hot welding.

[0012] Preferably, the hot welding mode comprises one of hot melt welding, high-frequency welding, ultrasonic welding and laser welding.

[0013] Preferably, the diaphragm is a PES ultrafiltration membrane with non-woven cloth support.

[0014] Preferably, the center tube and the outer tube are concentrically arranged, and the outer edge of the tube port of the center tube is arranged on the inner edge of the tube port of the outer tube.

[0015] Preferably, a limiting protrusion is arranged at the outer edge of the tube port of the center tube, a limiting groove is arranged at the inner edge of the tube port of the outer tube, and when the outer edge of the tube port of the center tube is arranged on the inner edge of the tube port of the outer tube, the limiting protrusion is arranged in the limiting groove.

[0016] Preferably, the outer surface of the pipe cap is provided with an anti-roll protrusion, which protrudes above the outer surface of the pipe cap and forms an anti-roll plane.

[0017] Preferably, the anti-roll plane is perpendicular to the line connecting the center of the tube cap.

[0018] Preferably, an air passage notch is provided at the outer edge of the central tube opening.

[0019] The beneficial effects of this utility model are as follows: The ultrafiltration centrifuge tube provided in this application integrates the first filter sheet on one side of the filtration section with the central tube, while the second filter sheet on the other side of the filtration section is fixed to the central tube by embedding and welding, ensuring sealing performance. Through the structural improvement of the central tube, on the one hand, the sealing performance between the separation membrane and the filter sheet, as well as between the filter sheet and the centrifuge tube, is ensured, avoiding leakage during high-speed centrifugation and ensuring the retention rate of the liquid. On the other hand, the structural improvement reduces the number of sealing and assembly steps for the components, thereby improving efficiency. Attached Figure Description

[0020] The above and other objects, features, and advantages of this invention will become clearer through a more detailed description of the preferred embodiments shown in the accompanying drawings. The same reference numerals indicate the same parts throughout the drawings, and the drawings are not intentionally drawn to scale with actual dimensions; the focus is on illustrating the gist of this invention.

[0021] Figure 1 This is a perspective view of the central tube of a preferred embodiment of the present invention;

[0022] Figure 2 This is a side view of the central tube of a preferred embodiment of the present invention;

[0023] Figure 3 for Figure 2 Sectional view at point AA';

[0024] Figure 4 This is a perspective view of the central tube without the second filter element welded to it, according to a preferred embodiment of the present invention.

[0025] Figure 5 This is a schematic diagram of the outer tube of a preferred embodiment of the present invention;

[0026] Figure 6 This is a schematic diagram of the pipe cap according to a preferred embodiment of the present utility model;

[0027] In the figure: 1. Pipe cap; 11. Anti-roll protrusion; 12. Anti-roll plane; 2. Central tube; 20. First chamber; 21. Annular part; 22. Filter part; 23. Limiting protrusion; 3. Outer tube; 33. Limiting groove; 4. First filter; 5. Second filter; 6. Membrane; 7. Air passage notch; 8. Hollow out. Detailed Implementation

[0028] To facilitate understanding of this utility model, a more comprehensive description of this utility model will be given below with reference to the accompanying drawings.

[0029] It should be noted that when a component is considered to be "connected" to another component, it can be directly connected to and integrated with the other component, or there may be an intervening component present. The terms "mounted," "one end," "the other end," and similar expressions used in this document are for illustrative purposes only.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0031] refer to Figures 1-6 This utility model provides an ultrafiltration centrifuge tube, including a tube body and a tube cap 1 covering the port of the tube body. The tube body includes a central tube 2 and an outer tube 3 sleeved outside the central tube 2. Both the central tube 2 and the outer tube 3 are tubular structures with an open top and a closed bottom. The central tube 2 and the outer tube 3 are concentrically arranged. The filter section 22 of the central tube 2 has a perforation 8. The outer port of the outer tube 3 has an external thread. The inner side of the tube cap 1 has an internal thread that matches the external thread. The central tube 2 has a first chamber 20. The gap between the outer wall of the central tube 2 and the inner wall of the outer tube 3 forms a second chamber. The central tube 2 includes an annular section 21 at the upper end and a filter section 22 at the lower end. A first filter 4 and a second filter 5 are arranged opposite each other on the filter section 22. The inner sides of the first filter 4 and the second filter 5 are both fixed with membranes 6 by welding. The first filter 4 is integrally formed with the filter section 22. The second filter 5 is fixed to the filter section 22 by welding. The filter section 22 is integrally formed with a first filter sheet 4. A membrane 6 is welded to the inner surface of the first filter sheet 4. A corresponding cutout 8 is provided on the filter section 22. A membrane 6 is welded to the inner surface of the second filter sheet 5. The second filter sheet 5 with the membrane 6 is embedded and secured in the cutout 8. Furthermore, the periphery of the second filter sheet 5 is fixed to the periphery of the cutout 8 by welding.

[0032] The first filter sheet 4 is integrally formed with the filter part 22 of the center pipe 2, so that secondary welding is not needed, and only the second filter sheet 5 is fixed at the hollow part 8 of the center pipe 2, and only one welding is needed, so that the process is simplified, and the product qualification rate is improved; moreover, the center pipe 2 structure of the application does not use adhesive, and there is no organic matter dissolution, and the dissolution is lower, so that the separated and purified components are relatively pure.

[0033] Reference Figure 2 In the preferred embodiment, the first filter sheet 4 is at an angle with the vertical direction, and the angle is 1-10℃, and the second filter sheet 5 is mirror-symmetric with the first filter sheet 4.

[0034] Reference Figure 3 The inner side of the first filter sheet 4 is fixed with a diaphragm 6; in the preferred embodiment, the diaphragm 6 is fixed with the first filter sheet 4 and the second filter sheet 5 by hot welding.

[0035] Reference Figure 1 The second filter sheet 5 is fixed on the center pipe 2 by hot welding, and the second filter sheet 5 completely covers the hollow part 8 on the filter part 22 of the center pipe 2.

[0036] Reference Figure 4 The filter part 22 of the center pipe 2 is provided with a hollow part 8, and the first filter sheet 4 is integrally formed on the filter part 22 of the center pipe 2.

[0037] In the preferred embodiment, the hot welding includes one of hot melting welding, high-frequency welding, ultrasonic welding and laser welding.

[0038] In the preferred embodiment, the diaphragm 6 is a PES ultrafiltration membrane with non-woven cloth support.

[0039] Reference Figure 1 , Figure 2 and Figure 4 In the preferred embodiment, the center pipe 2 and the outer pipe 3 are concentrically arranged, and the outer edge of the pipe opening of the center pipe 2 is arranged on the inner edge of the pipe opening of the outer pipe 3.

[0040] Reference Figure 1 and Figure 5 In the preferred embodiment, a limiting protrusion 23 is arranged at the outer edge of the pipe opening of the center pipe 2, and a limiting groove 33 is arranged at the inner edge of the pipe opening of the outer pipe 3, and when the outer edge of the pipe opening of the center pipe 2 is arranged on the inner edge of the pipe opening of the outer pipe 3, the limiting protrusion 23 is arranged in the limiting groove 33. Preferably, the limiting protrusion 23 is a strip-shaped protrusion, and the extension direction of the limiting protrusion 23 is perpendicular to the outer edge of the pipe opening of the center pipe 2.

[0041] ReferenceFigure 6 In the preferred embodiment, the outer surface of the tube cover 1 is provided with an anti-rolling boss 11, which is higher than the outer surface of the tube cover 1 and forms an anti-rolling plane 12.

[0042] Reference Figure 6 In the preferred embodiment, the anti-rolling plane 12 is perpendicular to the line connecting the center of the tube cover 1.

[0043] In the preferred embodiment, the center tube 2 is provided with a gas passing gap 7 at the outer edge of the tube mouth, which communicates the first chamber 20 and the space outside the tube body, so that the gas pressure inside and outside the tube body is balanced. The material of the center tube 2 is polymer plastic, and the material of the second filter sheet is polymer plastic.

[0044] The ultrafiltration centrifugal tube provided by the application guarantees that the centrifugal tube will not leak during use, guarantees the retention rate of the material liquid, provides an efficient separation method for the separation and concentration test of biological medicines and biological products, reduces the development cost of biological medicines, and guarantees the reliability of experimental data. In view of the leakage problem of the existing ultrafiltration centrifugal tube during use, the application provides a stable and reliable sealing and fixing method of the separation membrane and the plastic filter sheet, which shortens the welding process in the manufacturing process of the centrifugal tube, improves the yield of the centrifugal tube product, guarantees the sealing performance between the separation membrane and the filter sheet and between the filter sheet and the centrifugal tube, and avoids the leakage problem of the ultrafiltration centrifugal tube during high-speed centrifugation.

[0045] In the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be directly above or obliquely above the first feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "under" and "under" the second feature can be directly below or obliquely below the first feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0046] In the description of the specification, the description using the terms "preferred embodiment", "still another embodiment", "other embodiments", "specific example" or the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the specification, the illustrative expressions of the above terms are not necessarily directed to the same embodiment or example. Also, the specific features, structures, materials or characteristics described can be combined in an appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples, without contradiction.

[0047] Although the embodiments of the present application have been shown and described above, it is understood that the above-described embodiments are exemplary and are not to be construed as limiting the present application, and the person skilled in the art can make changes, modifications, replacements and variations to the above-described embodiments within the scope of the present application.

Claims

1. An ultrafiltration centrifuge tube characterized by, The application relates to a tube body and a tube cover arranged at the port of the tube body, wherein the tube body comprises a center tube and an outer tube sleeved outside the center tube, the center tube has a first cavity, a gap between the outer wall of the center tube and the inner wall of the outer tube forms a second cavity, the center tube comprises an annular part at the upper end and a filtering part at the lower end, the filtering part oppositely has a first filtering sheet and a second filtering sheet, the inner side of the first filtering sheet and the second filtering sheet is fixed with a diaphragm through welding, the first filtering sheet is integrally formed with the filtering part, and the second filtering sheet is fixed on the filtering part through welding.

2. The ultrafiltration centrifuge tube of claim 1, wherein, The first filtering sheet has an included angle with the vertical direction, and the included angle ranges from 1 to 10 DEG C.

3. The ultrafiltration centrifuge tube of claim 1, wherein, The diaphragm is fixed with the first filtering sheet and the second filtering sheet through hot welding, and the second filtering sheet is fixed on the center tube through hot welding.

4. The ultrafiltration centrifuge tube of claim 3, wherein, The hot welding mode comprises one of hot melt welding, high-frequency welding, ultrasonic welding and laser welding.

5. The ultrafiltration centrifuge tube of claim 1, wherein, The diaphragm is a PES ultrafiltration membrane with non-woven cloth support.

6. The ultrafiltration centrifuge tube of claim 1, wherein, The center tube and the outer tube are concentrically arranged, and the outer edge of the tube port of the center tube is arranged on the inner edge of the tube port of the outer tube.

7. The ultrafiltration centrifuge tube of claim 6, wherein, A gas passing gap is arranged at the outer edge of the tube port of the center tube.

8. The ultrafiltration centrifuge tube of claim 6, wherein, A limiting convex is arranged at the outer edge of the tube port of the center tube, and a limiting groove is arranged at the inner edge of the tube port of the outer tube, and when the outer edge of the tube port of the center tube is arranged on the inner edge of the tube port of the outer tube, the limiting convex is arranged in the limiting groove.

9. The ultrafiltration centrifuge tube of claim 8, wherein, An anti-rolling convex is arranged on the outer surface of the tube cover, the anti-rolling convex is higher than the outer surface of the tube cover and forms an anti-rolling plane.

10. The ultrafiltration centrifuge tube of claim 9, wherein, The anti-rolling plane is perpendicular to the connecting line of the center of the tube cover.

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