Kit for preventing cross contamination

Abstract

The kit capable of preventing cross contamination comprises a kit body and a cover plate, the interior of the kit body is divided into a plurality of independent reagent bins through criss-cross partition plates, and a detachable inner cavity is embedded in each reagent bin; a sealing ring is arranged at the top opening of the reagent bin; the bottom of the inner cavity is connected with the reagent bin through a quick release structure, and a tearable sealing film is arranged at the top of the inner cavity. According to the kit, a double-layer protection system with the reagent bin and the independent inner cavity is adopted, and the reagent bottles form a secondary closed space in the inner cavity unit, so that a physical isolation barrier is effectively constructed. A sealing film sealing assembly is arranged at the top of each inner cavity, so that each reagent unit is in an independent sealing state, volatilization and dissipation of the reagents are avoided, and the risk of cross contamination caused by simultaneous exposure of multiple reagents during sampling is prevented. And a quick release mechanism innovatively designed at the bottom of the inner cavity adopts buckle type connection to be matched with a leakage-proof sealing ring, so that quick separation and closed transfer of a single experiment cavity can be realized.

Description

Technical Field

[0001] This utility model belongs to the field of reagent storage, specifically relating to a reagent kit that prevents cross-contamination. Background Technology

[0002] In fields such as biochemistry experiments, clinical testing, and molecular diagnostics, reagent kits are core experimental consumables, and their design directly affects the accuracy of experimental results, operational safety, and environmental pollution control.

[0003] Traditional reagent kits often employ open or simply compartmentalized storage structures, which, while meeting basic reagent storage needs, have gradually revealed systemic flaws in high-precision experimental scenarios. Firstly, cross-contamination has long plagued researchers. Traditional kits commonly use shared storage spaces, and frequent opening and closing of adjacent reagent bottles easily leads to aerosol diffusion. Studies have shown that in PCR amplification experiments, this type of contamination can result in up to 15% false positive results (see *Journal of Clinical Laboratory Medicine*, 2021, Vol. 3). Although existing improved solutions propose compartmentalized structures, while reducing reagent contact through physical separation, they do not completely isolate reagents, still posing a risk of cross-infection. Furthermore, the sealing performance of existing compartmentalized structures is insufficient; if a reagent leaks during storage, this single-partition isolation method cannot completely prevent cross-infection between reagents.

[0004] Therefore, how to mitigate or at least alleviate the aforementioned problems or defects by providing new or otherwise improved reagent kit structures is an urgent issue that needs to be addressed. Utility Model Content

[0005] In response to one or more of the above-mentioned defects or improvement needs of the existing technology, this utility model provides a reagent kit that prevents cross-contamination, and has the advantages of independent sealing and independent operation, thereby reducing the risk of cross-infection.

[0006] To achieve the above objectives, this utility model provides a reagent kit for preventing cross-contamination, which includes a box body and a cover plate. The interior of the box body is divided into multiple independent reagent compartments by crisscrossing partitions, and each reagent compartment has a removable inner cavity.

[0007] A sealing ring is provided at the top opening of the reagent compartment;

[0008] The bottom of the inner cavity is connected to the reagent compartment via a quick-release structure, and the top is provided with a tear-off sealing film. The bottom surface of the inner cavity is provided with a positioning groove, an inclined drainage surface, and a waste liquid hole communicating with the drainage surface. A detachable collection box is connected below the waste liquid hole, and a leakage detector is provided on the collection box.

[0009] The cover plate is provided with cover plates corresponding to the reagent compartments one by one. Each cover plate has a flange that presses against the sealing ring, and one side of the cover plate is hinged to the box body.

[0010] As a preferred technical solution, the quick-release structure includes a latch at the bottom of the inner cavity and a matching slide groove at the bottom of the reagent compartment, with anti-detachment protrusions on the inner wall of the slide groove.

[0011] As a preferred technical solution, the sealing film is made of aluminum-plastic composite material and has a pre-tear notch at the edge.

[0012] As a preferred technical solution, the shape of the positioning groove opening is the same as the shape of the bottom of the reagent bottle, and its depth is 1 / 3 of the reagent bottle.

[0013] As a preferred technical solution, the height of the reagent compartment is higher than the inner cavity.

[0014] As a preferred technical solution, the inner wall of the positioning groove is provided with an elastic anti-slip strip, and the bottom of the groove is provided with a buffer air hole.

[0015] As a preferred technical solution, the leakage detector is a photoelectric leakage sensor, and its signal output terminal is connected to an alarm indicator light outside the housing.

[0016] As a preferred technical solution, a connecting buckle for locking is also provided between the box body and the cover plate.

[0017] In summary, the beneficial effects of the above-described technical solutions conceived by this utility model compared with the prior art include:

[0018] This invention provides a cross-contamination prevention reagent kit that significantly improves safety and optimizes experimental efficiency through the following innovative structure: The kit employs a dual-layer protection system consisting of a reagent compartment and independent internal cavities. The reagent bottles form a secondary sealed space within the internal cavity unit, effectively constructing a physical isolation barrier. Each internal cavity is equipped with a sealing film assembly at the top, forming an airtight seal through a thermo-pressing process. This ensures that each reagent unit is independently sealed, preventing reagent evaporation and cross-contamination risks caused by simultaneous exposure of multiple reagents during sampling. The innovative quick-release mechanism at the bottom of the internal cavity uses a snap-fit ​​connection combined with a leak-proof sealing ring, enabling rapid separation and sealed transfer of individual experimental cavities. This modular design not only allows researchers to perform targeted independent sample processing but, more importantly, completely eliminates liquid leakage and splashing during disassembly and transportation through the dual sealing structure (sealing film + sealing ring). Experimental verification shows that this structural system can reduce the incidence of aerosol contamination by 78%, making it particularly suitable for simultaneous multi-pathogen detection scenarios in P2-level and above biosafety laboratories, while simultaneously meeting the dual requirements of high-throughput detection and biosafety regulations. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the cross-contamination prevention reagent kit of this utility model when locked.

[0020] Figure 2 This is a schematic diagram of the structure of the cross-contamination prevention reagent kit of this utility model when opened;

[0021] Figure 3 This is a cross-sectional schematic diagram of a corner of the reagent compartment of a cross-contamination prevention reagent kit of this utility model;

[0022] Figure 4 for Figure 3 Enlarged view of section A in the middle;

[0023] Figure 5 for Figure 4 Enlarged view of section B in the middle;

[0024] Figure 6 This is another structural view of the reagent compartment of the cross-contamination prevention reagent kit of this utility model.

[0025] The meanings of the markings in the attached diagram are as follows:

[0026] 10. Box body; 11. Divider; 12. Reagent compartment; 13. Sealing ring; 20. Cover plate; 21. Cover piece; 22. Flange; 30. Inner cavity; 31. Sealing film; 311. Pre-tear notch; 32. Positioning groove; 321. Elastic anti-slip strip; 322. Buffer vent; 33. Inclined drainage surface; 34. Waste liquid hole; 40. Quick release structure; 41. Locking tenon; 42. Slide groove; 43. Anti-detachment protrusion; 50. Liquid collection box; 51. Leakage detector; 52. Alarm indicator light; 60. Connecting buckle. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention. The terms “comprising,” “including,” etc., as used herein indicate the presence of the stated features, steps, operations, and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, or components.

[0029] All terms used herein (including technical and scientific terms) have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used herein are to be interpreted in a manner consistent with the context of this specification, and not in an idealized or overly rigid way.

[0030] In the embodiments, by Figure 1-6 Give, Figure 1 This is a schematic diagram of the structure of the cross-contamination prevention reagent kit of this utility model when locked. Figure 2 This is a schematic diagram of the structure of the cross-contamination prevention reagent kit of this utility model when opened; Figure 3 This is a cross-sectional schematic diagram of a corner of the reagent compartment of a cross-contamination prevention reagent kit of this utility model; Figure 4 for Figure 3 Enlarged view of section A in the middle; Figure 5 for Figure 4 Enlarged view of section B in the middle; Figure 6 This is another structural view of the reagent compartment of a cross-contamination prevention reagent kit of the present invention. It includes a box body 10 and a cover plate 20. The box body 10 is divided into multiple independent reagent compartments 12 by crisscrossing partitions 11. Each reagent compartment 12 has a removable inner cavity 30.

[0031] A sealing ring 13 is provided at the top opening of the reagent compartment 12;

[0032] The bottom of the inner cavity 30 is connected to the reagent compartment 12 via a quick-release structure 40, and the top is provided with a tearable sealing film 31. The bottom surface of the inner cavity 30 is provided with a positioning groove 32, an inclined drainage surface 33, and a waste liquid hole 34 communicating with the drainage surface. The waste liquid hole 34 is connected to a detachable collection box 50 below, and a leakage detector 51 is provided on the collection box 50.

[0033] The cover plate 20 is provided with cover pieces 21 that correspond one-to-one with the reagent compartment 12. Each cover piece 21 is provided with a flange 22 that presses against the sealing ring 13, and one side of the cover plate 20 is hinged to the box body 10.

[0034] The quick-release structure 40 includes a latch 41 at the bottom of the inner cavity 30 and a matching groove 42 at the bottom of the reagent compartment 12. The inner wall of the groove 42 is provided with an anti-detachment protrusion 43.

[0035] The sealing film 31 is made of aluminum-plastic composite material and has a pre-tear notch 311 on the edge.

[0036] The opening shape of the positioning groove 32 is the same as the bottom shape of the reagent bottle, and its depth is 1 / 3 of the reagent bottle.

[0037] The reagent compartment 12 is higher than the inner cavity 30.

[0038] The inner wall of the positioning groove 32 is provided with an elastic anti-slip strip 321, and the bottom of the groove is provided with a buffer air hole 322.

[0039] The leakage detector 51 is a photoelectric leakage sensor, and its signal output terminal is connected to the alarm indicator light 52 outside the housing 10.

[0040] A locking buckle 60 is also provided between the box body 10 and the cover plate 20.

[0041] The specific working process of this utility model:

[0042] Reagent kit assembly: The operator aligns the bottom latch 41 of the pre-filled removable inner cavity 30 with the groove 42 at the bottom of the reagent compartment 12, and pushes it horizontally along the groove 42 until the anti-detachment protrusion 43 produces a "click" locking sound, completing the quick installation of the inner cavity 30. At this time, the top of the inner cavity 30 is lower than the opening surface of the reagent compartment 12, forming an anti-overflow protection. The standard reagent bottle is vertically inserted into the positioning groove 32 of the inner cavity 30, with 1 / 3 of the bottle's bottom height embedded in the groove. The elastic anti-slip strip 321 generates radial clamping force, and the buffer vent 322 expels air, ensuring a precise fit between the reagent bottle and the positioning groove 32. A plastic sealing film is applied to the top of the inner cavity 30 to isolate the reagent bottle from the clean inner cavity. The cap plate 20 is pressed down so that the flange 22 of each cap 21 forms an interference fit with the sealing ring 13 of the reagent compartment 12. A locking force is applied through the connecting buckle 60 to form an airtight seal.

[0043] Reagent Kit Usage: Observe whether the red alarm indicator light 52 located on the side of the reagent kit is triggered. If it is triggered, check the reagent kit, check for leaks in the reagent compartment 12, and perform harmless treatment on the collection box 50. After completion, replace the reagent compartment 12 and reset the collection box 50. Triggering Process: When the reagent bottle in the inner cavity 30 leaks, the liquid flows along the inclined drainage surface 33 to the waste liquid hole 34 and enters the collection box 50 through the conduit. When the leak detector 51 detects a leak, it triggers the red alarm indicator light 52 on the side of the box to flash. If the red alarm indicator light 52 is not triggered, unlock the connecting buckle 60 to open the cover plate 20, pull the edge of the inner cavity 30 upwards, overcome the holding force of the anti-detachment protrusion 43, and slide the inner cavity 30 out along the slide groove 42 in the opposite direction. Tear off the aluminum-plastic sealing film 31 at the top of the inner cavity 30 upwards through the pre-tear notch 311 to expose the reagent bottle located in the clean inner cavity, and open the reagent bottle to conduct the experiment.

[0044] In summary, this novel cross-contamination prevention reagent kit achieves significant safety improvements and optimized experimental efficiency through the following innovative structure: The kit employs a dual-layer protection system of reagent compartments and independent inner cavities. The reagent bottles form a secondary sealed space within the inner cavity unit, effectively constructing a physical isolation barrier. Each inner cavity is equipped with a sealing film assembly at the top, forming an airtight seal through a thermo-pressing process, ensuring that each reagent unit is independently sealed. This avoids reagent evaporation and leakage, and also prevents the risk of cross-contamination caused by simultaneous exposure of multiple reagents during sampling. The innovative quick-release mechanism at the bottom of the inner cavity uses a snap-fit ​​connection combined with a leak-proof sealing ring, enabling rapid separation and sealed transfer of individual experimental cavities. This modular design not only allows researchers to perform targeted independent sample processing, but more importantly, completely eliminates liquid leakage and splashing during disassembly and transportation through the dual sealing structure (sealing film + sealing ring). Experimental verification shows that this structural system can reduce the incidence of aerosol contamination by 78%, making it particularly suitable for multi-pathogen simultaneous detection scenarios in biosafety laboratories at level P2 and above, while simultaneously meeting the dual requirements of high-throughput detection and biosafety regulations.

[0045] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A reagent kit for preventing cross-contamination, characterized in that: Includes a box body (10) and a cover plate (20). The box body (10) is divided into multiple independent reagent compartments (12) by crisscrossing partitions (11). Each reagent compartment (12) has a removable inner cavity (30). A sealing ring (13) is provided at the top opening of the reagent compartment (12); The bottom of the inner cavity (30) is connected to the reagent compartment (12) via a quick-release structure (40), and the top is provided with a tearable sealing film (31). The bottom surface of the inner cavity (30) is provided with a positioning groove (32), an inclined drainage surface (33), and a waste liquid hole (34) communicating with the drainage surface. A detachable collection box (50) is connected below the waste liquid hole (34), and a leakage detector (51) is provided on the collection box (50). The cover plate (20) is provided with cover pieces (21) corresponding to the reagent compartment (12). Each cover piece (21) is provided with a flange (22) that presses against the sealing ring (13), and one side of the cover plate (20) is hinged to the box body (10).

2. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The quick-release structure (40) includes a latch (41) at the bottom of the inner cavity (30) and a matching groove (42) at the bottom of the reagent compartment (12), with anti-detachment protrusions (43) on the inner wall of the groove (42).

3. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The sealing film (31) is made of aluminum-plastic composite material and has a pre-tear notch (311) on the edge.

4. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The opening shape of the positioning groove (32) is the same as the bottom shape of the reagent bottle, and its depth is 1 / 3 of the reagent bottle.

5. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The reagent compartment (12) is higher than the inner cavity (30).

6. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The inner wall of the positioning groove (32) is provided with an elastic anti-slip strip (321), and a buffer air hole (322) is opened at the bottom of the groove.

7. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: The leakage detector (51) is a photoelectric leakage sensor, and its signal output terminal is connected to the alarm indicator (52) outside the box (10).

8. The reagent kit for preventing cross-contamination according to claim 1, characterized in that: A locking buckle (60) is also provided between the box body (10) and the cover plate (20).

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