Cell separating and screening device

Abstract

The utility model discloses a cell separating and screening device and relates to the technical field of cell separation.The cell separating and screening device comprises a separating and screening box, side sliding grooves are formed in the two sides of the separating and screening box, cell preservation liquid flows out of an end through groove through the arrangement of the end through groove in an upward discharging mode, then the cell preservation liquid is gathered through a guide rod, and then the cell preservation liquid is separated and screened. The cell preservation liquid drips, the filtered cells are impacted, the gathered cells are dispersed, the cells are driven to move by utilizing the mobility of the cell preservation liquid, the screened cells can be screened out of the filtering frame, the screening effect is ensured, in this way, multiple times of screening are not needed, and the cell preservation liquid flows out from the upper portion through the design, so that the screening efficiency is improved. Separated cells can be prevented from entering a connecting pipe, waste is avoided, practicability is higher, a second driving motor is arranged to drive the connecting pipe to move, the cells are spread out, the utilization rate of the filtering frame is increased, and the filtering effect is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell separation technical field especially, relate to a cell separation screening device. BACKGROUND

[0002] Cell separation technology includes centrifugal technology, flow cytometry and cell electrophoresis. Centrifugation is a basic means for studying organelles such as cell nuclei, mitochondria, Golgi bodies, lysosomes and microbodies, as well as various macromolecules. Flow cytometry is a technique for rapid quantitative analysis and sorting of individual cells. Cell electrophoresis refers to the fact that cells have a net positive or negative charge on their surface at a certain pH value, and can move under the action of an external electric field.

[0003] In the prior art, for example, Chinese patent CN220724161U discloses a cell separation screening device, which comprises a sleeve, a mounting plate is installed on the outer surface of the sleeve, a support rod is installed on the lower surface of the mounting plate, a closing device is installed on the upper surface of the sleeve, a feeding pipe is arranged above the closing device, a cavity is formed in the sleeve, a rotating shaft is installed on the bottom of the inner wall of the cavity, a fixed plate is installed on the upper end of the rotating shaft, a circular separation plate is installed on the surface of the fixed plate, a separation groove is formed in the surface of the circular separation plate, an extension rod is installed on the top of the inner wall of the cavity, a cleaning plate is installed on the outer surface of the extension rod, a support is installed inside the cavity, an arc plate is installed on the side of the support, a plurality of filter plates are installed inside the cavity, a discharge pipe is installed on the side of the sleeve, and a discharge pipe is installed on the lower surface of the sleeve.

[0004] However, in the prior art, the screen is usually flat during use, and the unfiltered cells tend to accumulate during the filtering process. If the screen is inclined, the filtered cells will flow out directly along the inclined surface, resulting in poor filtering effect and the need for secondary or even tertiary filtering, which reduces the efficiency of cell separation and screening and is inconvenient to use. UTILITY MODEL CONTENT

[0005] The utility model aims at solving the problems in the prior art that the screen is usually flat during use, and the unfiltered cells tend to accumulate during the filtering process. If the screen is inclined, the filtered cells will flow out directly along the inclined surface, resulting in poor filtering effect and the need for secondary or even tertiary filtering, which reduces the efficiency of cell separation and screening and is inconvenient to use. The utility model provides a cell separation screening device.

[0006] In order to achieve the above object, the utility model discloses the following technical scheme: a cell separation and screening device, including separation and screening box, both sides of separation and screening box are all seted up with side slide groove, the inside of two side slide grooves all are slidably connected with sliding block, two sliding blocks are fixedly connected with connecting pipe, the upper end of connecting pipe is seted up with end through groove, one side of one sliding block is fixedly connected with connecting port, the connecting port is communicated with connecting pipe, the one side of separation and screening box is seted up with connecting hole, the middle one side of separation and screening box inside is fixedly connected with guide light pole, the middle other side of separation and screening box inside is rotatably connected with screw rod, the upper end of two sliding blocks all is fixedly connected with connecting block, the screw rod is screwed with one connecting block, and another connecting block is slidably connected with guide light pole, the one side of separation and screening box is fixedly connected with no.

[0007] Preferably, the lower end of the connecting pipe is fixedly connected with a guide rod, and the two sides of the separation and screening box are fixedly connected with side sealing plates.

[0008] Preferably, the upper middle part of the separation and screening box inside is fixedly connected with a bottom support frame, the upper end of the bottom support frame is rotatably connected with a separation barrel, and the upper end of the separation barrel is rotatably connected with the separation and screening box.

[0009] Preferably, the upper middle part of the separation and screening box is fixedly connected with a feeding port, the feeding port is communicated with the separation barrel, the upper end of the separation and screening box is fixedly connected with a first driving motor, the output end of the first driving motor is fixedly connected with a first gear through the separation and screening box, the upper end of the outer surface of the separation barrel is fixedly connected with a second gear, and the first gear is engaged with the second gear.

[0010] Preferably, the lower end of the bottom support frame is provided with a guide hopper, and the guide hopper is fixedly connected with the separation and screening box.

[0011] Preferably, the lower middle part of the separation and screening box inside is seted up with slide cavity, the lower middle part of the separation and screening box inside is fixedly connected with inner support frame, the inner support frame is located at both ends of slide cavity, the inside of slide cavity is slidably connected with filter frame, the filter frame is located below side slide groove, and the inside of separation and screening box is provided with limit pipe rod, and the upper end of limit pipe rod is fixedly connected with inner support frame.

[0012] Preferably, one side of the filter frame is fixedly connected with a handle, four corners of the lower end of the separation and screening box are fixedly connected with supporting legs, one side of the separation and screening box is fixedly connected with a first discharge port, the first discharge port is located above the filter frame, and a second discharge port is fixedly connected to the middle of one side of the separation and screening box.

[0013] Compared with the prior art, the cell separation and screening device has the advantages and positive effects that:

[0014] 1、In the utility model, through the setting of the end through groove, the cell preservation solution flows out from the end through groove in an upward manner, and then the cell preservation solution is gathered through the guide rod, so that the filtered cells are impacted, the gathered cells are scattered, the flowability of the cell preservation solution is utilized to drive the cells to move, the cells that can be screened out can be screened out from the filter frame, the screening effect is guaranteed, in this way, multiple screening is not needed, the cell preservation solution flows out from the top, the separated cells can be prevented from entering the connecting pipe, waste is avoided, and the practicability is higher, through the setting of the second driving motor, the connecting pipe is driven to move, the cells are spread, the utilization rate of the filter frame is increased, and the filtering effect is guaranteed.

[0015] 2、In the utility model, through the setting of the inner support frame, the support intensity of the filter frame is increased, and the stability of the filter frame in use is guaranteed, through the setting of the limiting pipe rod, the pipe connected with the connecting port is limited, and the pipe is prevented from moving to the top of the filter frame along with the movement of the connecting pipe. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 A three-dimensional structure diagram of the cell separation and screening device is provided for the utility model Figure 1 ;

[0017] Figure 2 A three-dimensional structure diagram of the cell separation and screening device is provided for the utility model Figure 2 ;

[0018] Figure 3 A partial structure explosion view of the cell separation and screening device is provided for the utility model

[0019] Figure 4 An internal structure diagram of the separation and screening box in the cell separation and screening device is provided for the utility model

[0020] Figure 5 A three-dimensional structure diagram of the connecting pipe (22) in the cell separation and screening device is provided for the utility model.

[0021] Legend: 1. Separation and screening box; 2. Feed inlet; 3. Drive motor No. 1; 4. Discharge port No. 1; 5. Side sealing plate; 6. Filter frame; 7. Connecting hole; 8. Handle; 9. Support leg; 10. Discharge port No. 2; 11. Drive motor No. 2; 12. Sliding cavity; 13. Side sliding groove; 14. Inner support frame; 15. Guide rod; 16. Feeding funnel; 17. Separation barrel; 18. Bottom support frame; 19. Gear No. 1; 20. Gear No. 2; 21. Sliding block; 22. Connecting pipe; 23. Guide rod; 24. Lead screw; 25. Connecting block; 26. End slot; 27. Connecting port; 28. Limiting rod. Detailed Implementation

[0022] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0023] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0024] Example 1: As Figure 1 - Figure 5 As shown, this utility model provides a cell separation and screening device, including a separation and screening box 1. Side sliding grooves 13 are provided on both sides of the separation and screening box 1. Sliding blocks 21 are slidably connected inside each of the two side sliding grooves 13. A connecting pipe 22 is fixedly connected between the two sliding blocks 21. An end-through groove 26 is provided at the upper end of the connecting pipe 22. A connecting port 27 is fixedly connected to one side of one of the sliding blocks 21, communicating with the connecting pipe 22. A connecting hole 7 is provided on one side of the separation and screening box 1. A guide light rod 15 is fixedly connected to one side of the middle part of the interior of the separation and screening box 1. A guide light rod 15 is fixedly connected to the other side of the middle part of the interior of the separation and screening box 1. A lead screw 24 is rotatably connected to the side. Connecting blocks 25 are fixedly connected to the upper ends of the two sliders 21. The lead screw 24 is threadedly connected to one of the connecting blocks 25, and the other connecting block 25 is slidably connected to the guide light rod 15. A second drive motor 11 is fixedly connected to one side of the separation and screening box 1. The output end of the second drive motor 11 is fixedly connected to one end of the lead screw 24. The connection port 27 is used to connect the pipe. The pipe passes through the connection hole 7 and enters the separation and screening box 1. The pipe is connected to the output end of the pump. The pump pumps cell preservation solution into the connecting pipe 22. A guide rod 23 is fixedly connected to the lower end of the connecting pipe 22.

[0025] The specific settings and functions of this embodiment are described in detail below. Through the end channel 26, the cell preservation solution flows out from the top, and then is gathered by the guide rod 23, causing it to drip and impact the filtered cells, dispersing the gathered cells. The fluidity of the cell preservation solution moves the cells, ensuring that cells that can be screened are screened out of the filter rack 6, guaranteeing the screening effect. This method eliminates the need for multiple screenings. Furthermore, this design, with the cell preservation solution flowing out from above, prevents separated cells from entering the connecting tube 22, avoiding waste and increasing practicality. The second drive motor 11 moves the connecting tube 22, spreading the cells and increasing the utilization rate of the filter rack 6, ensuring the filtration effect.

[0026] Example 2: Figure 1 - Figure 5 As shown, side sealing plates 5 are fixedly connected to both sides of the separation and screening box 1. The side sealing plates 5 are used to block the side sliding grooves 13. A bottom support frame 18 is fixedly connected to the upper middle part of the interior of the separation and screening box 1. A separation barrel 17 is rotatably connected to the upper end of the bottom support frame 18. The upper end of the separation barrel 17 is rotatably connected to the separation and screening box 1. A feed inlet 2 is fixedly connected to the upper middle part of the separation and screening box 1. The feed inlet 2 communicates with the separation barrel 17. A first drive motor 3 is fixedly connected to the upper end of the separation and screening box 1. A first gear 19 is fixedly connected to the output end of the first drive motor 3 through the separation and screening box 1. A second gear 20 is fixedly connected to the upper end of the outer surface of the separation barrel 17. The first gear 19 and the second gear 20 mesh. A guide funnel 16 is provided below the bottom support frame 18. The guide funnel 16 is fixedly connected to the separation and screening box 1. A sliding cavity 12 is opened in the lower middle part of the interior of the separation and screening box 1. An inner support frame 14 is fixedly connected to the lower middle part of the interior of the separation screening box 1. The inner support frame 14 is located at both ends of the sliding cavity 12. A filter frame 6 is slidably connected inside the sliding cavity 12. The filter frame 6 is located below the side sliding groove 13. A limiting rod 28 is set inside the separation screening box 1. The limiting rod 28 is fixedly connected to the upper end of the inner support frame 14. A handle 8 is fixedly connected to one side of the filter frame 6. Support feet 9 are fixedly connected to the four corners of the lower end of the separation screening box 1. A discharge port 4 is fixedly connected to one side of the separation screening box 1. The discharge port 4 is located above the filter frame 6. A discharge port 10 is fixedly connected to the lower middle part of one side of the separation screening box 1. The filter frame 6 at the bottom is used to separate cells from cell preservation solution. The limiting rod 28 is located inside the separation screening box 1 on the same side as the connecting hole 7. The limiting rod 28 is located below the slider 21. The upper end of the limiting rod 28 is located below the slider 21.

[0027] The overall effect of this embodiment is that the inner support frame 14 is used to increase the support strength of the filter frame 6 and ensure the stability of the filter frame 6 during use. The pipe limiting rod 28 is used to restrict the pipe connected to the connection port 27 and prevent the pipe from moving above the filter frame 6 as the connection pipe 22 moves.

[0028] The usage and working principle of this device are as follows: When in use, cells enter through the inlet 2. The first drive motor 3 drives the separation tank 17 to rotate, separating the cells. The separated cells then fall onto the surface of the uppermost filter rack 6 through the guide funnel 16. The second drive motor 11 drives the connecting pipe 22 to move. At the same time, the cell preservation solution flows out from the end channel 26. The cell preservation solution flows onto the surface of the connecting pipe 22, is collected by the guide rod 23, and then drips down, breaking up the clustered cells and ensuring the cell screening effect. The screened cells located above the filter rack 6 flow out from the first discharge port 4. The cell solution is screened out through the next filter rack 6 and flows out from the second discharge port 10.

[0029] The above are merely preferred embodiments of this utility model and are not intended to limit the utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model without departing from the technical solution of this utility model shall still fall within the protection scope of this utility model.

Claims

1. A cell separation and screening device, comprising a separation and screening chamber (1), characterized in that: The separation and screening box (1) has side sliding grooves (13) on both sides, and sliders (21) are slidably connected inside the two side sliding grooves (13). A connecting pipe (22) is fixedly connected between the two sliders (21). An end through groove (26) is opened at the upper end of the connecting pipe (22). A connecting port (27) is fixedly connected to one side of one of the sliders (21). The connecting port (27) communicates with the connecting pipe (22). A connecting hole (7) is opened on one side of the separation and screening box (1). The middle part of the separation and screening box (1) is connected to the connecting pipe (22). A guide rod (15) is fixedly connected to one side of the separation and screening box (1). A lead screw (24) is rotatably connected to the other side of the middle part of the separation and screening box (1). A connecting block (25) is fixedly connected to the upper end of each of the two sliders (21). The lead screw (24) is threadedly connected to one of the connecting blocks (25), and the other connecting block (25) is slidably connected to the guide rod (15). A second drive motor (11) is fixedly connected to one side of the separation and screening box (1). The output end of the second drive motor (11) is fixedly connected to one end of the lead screw (24).

2. The cell separation and screening device according to claim 1, characterized in that: The lower end of the connecting pipe (22) is fixedly connected to a guide rod (23), and both sides of the separation screening box (1) are fixedly connected to side sealing plates (5), which are used to block the side sliding groove (13).

3. The cell separation and screening device according to claim 1, characterized in that: A bottom support frame (18) is fixedly connected to the upper middle part of the interior of the separation and screening box (1). A separation bucket (17) is rotatably connected to the upper end of the bottom support frame (18). The upper end of the separation bucket (17) is rotatably connected to the separation and screening box (1).

4. The cell separation and screening device according to claim 1, characterized in that: The upper middle part of the separation and screening box (1) is fixedly connected to the feed inlet (2), which is connected to the separation barrel (17). The upper end of the separation and screening box (1) is fixedly connected to the first drive motor (3). The output end of the first drive motor (3) passes through the separation and screening box (1) and is fixedly connected to the first gear (19). The upper end of the outer surface of the separation barrel (17) is fixedly connected to the second gear (20), which meshes with the first gear (19) and the second gear (20).

5. The cell separation and screening device according to claim 3, characterized in that: A material guide funnel (16) is provided below the bottom support frame (18), and the material guide funnel (16) is fixedly connected to the separation and screening box (1).

6. The cell separation and screening device according to claim 1, characterized in that: A sliding cavity (12) is provided in the lower middle part of the interior of the separation and screening box (1). An inner support frame (14) is fixedly connected in the lower middle part of the interior of the separation and screening box (1). The inner support frame (14) is located at both ends of the sliding cavity (12). A filter frame (6) is slidably connected inside the sliding cavity (12). The filter frame (6) is located below the side sliding groove (13). A limiting tube rod (28) is provided inside the separation and screening box (1). The limiting tube rod (28) is fixedly connected to the upper end of the inner support frame (14).

7. The cell separation and screening device according to claim 6, characterized in that: A handle (8) is fixedly connected to one side of the filter frame (6), and four corners of the lower end of the separation screening box (1) are fixedly connected to support legs (9). A first discharge port (4) is fixedly connected to one side of the separation screening box (1), and the first discharge port (4) is located above the filter frame (6). A second discharge port (10) is fixedly connected to the lower middle part of one side of the separation screening box (1).

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