Device for isolated culture of oral microorganisms

Abstract

The utility model relates to the technical field of bioengineering, in particular to a device for isolated culture of oral microorganisms. The device for isolated culture of oral microorganisms sequentially comprises a first filter membrane, a micropore array chip and a second filter membrane from bottom to top, the first filter membrane or the second filter membrane is used for being arranged on teeth of a patient; wherein the micropore array chip is provided with a plurality of through culture micropores, the first filter membrane covers the bottoms of all the culture micropores, the second filter membrane covers the tops of all the culture micropores, and the culture micropores are used for growth and reproduction of oral microorganisms. The device can ensure that the growth of oral microorganisms fits the growth condition of original ecological microorganisms, does not influence the growth speed of the oral microorganisms, does not need to consider nutrient requirements, and is high in practicability and suitable for large-area popularization.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and in particular to a device for isolating and culturing oral microorganisms. Background Technology

[0002] The oral microbial community is an important component of the human microbial community. The balance of the oral microbial community directly affects various health conditions such as periodontal disease, dental caries, and overall health. In the medical field, microbial isolation and culture devices can be used to diagnose and treat diseases. The oral microorganisms obtained can not only help us understand the pathogenesis and pathological process of oral diseases, but also provide new ideas and methods for the prevention and treatment of oral diseases.

[0003] Currently, most methods for isolating and culturing oral microorganisms rely on traditional culture media and culture devices. These methods often have problems such as complex operation, long culture cycle, and high risk of contamination. Furthermore, the natural habitat of oral microorganisms is complex, and existing technologies cannot completely replicate the original environment of oral microorganisms.

[0004] Therefore, providing a device for isolating and culturing oral microorganisms is of great significance for the identification and research of oral microorganisms. Utility Model Content

[0005] The technical problem to be solved by this utility model is to provide a device for isolating and culturing oral microorganisms.

[0006] To solve the above problems, the technical solution adopted by this utility model is as follows:

[0007] An apparatus for isolating and culturing oral microorganisms, comprising, from bottom to top: a first filter membrane, a microporous array chip, and a second filter membrane; the first filter membrane or the second filter membrane is used to be applied to a patient's teeth;

[0008] The micropore array chip has several interconnected culture micropores. The first filter membrane covers the bottom of all culture micropores, and the second filter membrane covers the top of all culture micropores. The culture micropores are used for the growth and reproduction of oral microorganisms.

[0009] In one embodiment of this utility model, both the first filter membrane and the second filter membrane are bonded to the microporous array chip.

[0010] In one embodiment of this utility model, the pore size of both the first filter membrane and the second filter membrane is 0.03 μm.

[0011] In one embodiment of this utility model, the micropore array chip is provided with multiple micropore arrays, and each micropore array contains multiple culture micropores.

[0012] As one embodiment of this utility model, the pore size of the culture micropores in different micropore arrays is different.

[0013] In one embodiment of this utility model, the micro-pore array chip is made of polypropylene.

[0014] In one embodiment of this utility model, the micro-hole array chip has a length of 5-7mm, a width of 3-5mm, and a thickness of 1-2mm.

[0015] In one embodiment of this utility model, the thickness of the first filter membrane and the second filter membrane is 15-20 μm.

[0016] In one embodiment of this utility model, both the first filter membrane and the second filter membrane are made of polycarbonate.

[0017] The beneficial effects of adopting the above technical solution are as follows:

[0018] The oral microbial isolation and culture device provided in this embodiment has a simple structure and is easy to operate. The device can ensure that the growth of oral microorganisms is consistent with the growth of original microorganisms, without affecting the growth rate of oral microorganisms, and without considering the issue of nutrient requirements. The device is highly practical and suitable for large-scale promotion. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of an oral microbial isolation and culture device provided in an embodiment of the present invention.

[0020] Figure 2 yes Figure 1 An explosion diagram.

[0021] Figure 3 This is a top view of a micro-hole array chip provided in an embodiment of this utility model.

[0022] Figure 4 This is a top view of another micro-hole array chip provided in this embodiment of the present invention.

[0023] Wherein: 1 is the first filter membrane, 2 is the micropore array chip, 3 is the second filter membrane, 201 is the culture micropore, and 202 is the micropore array. Detailed Implementation

[0024] To make the objectives, technical solutions and advantages of this utility model clearer, the utility model will be clearly and completely described below in conjunction with specific embodiments.

[0025] This utility model provides a device for isolating and culturing oral microorganisms, such as... Figures 1-4As shown, it comprises, from bottom to top: a first filter membrane 1, a microporous array chip 2, and a second filter membrane 3; the first filter membrane 1 or the second filter membrane 3 is used to be specifically bonded to the patient's teeth;

[0026] The micropore array chip 2 is provided with a plurality of through-hole culture micropores 201. The first filter membrane 1 covers the bottom of all culture micropores 201, and the second filter membrane 3 covers the top of all culture micropores 201. The culture micropores 201 are used for microbial growth and reproduction.

[0027] In use, firstly, the second filter membrane 3 is adhered to the lower side of the microporous array chip 2 using medical self-adhesive silicone; then, LB solid culture medium is prepared and sterilized at 121°C for 30 minutes. When the temperature drops to about 55°C, it is placed in a petri dish, and the device is immersed in the LB solid culture medium. After the culture medium solidifies, it is removed and the surrounding residual culture medium is removed.

[0028] Saliva is injected into the culture micropore 201 using a disposable syringe. The first filter membrane 1 is then adhered to the upper side of the micropore array chip 2 using medical self-adhesive silicone. After the medical self-adhesive silicone has completely cured, the oral microbial isolation and culture device is obtained. The first filter membrane 1 or the second filter membrane 3 is then bonded to the buccal side of the patient's maxillary first molar using dental adhesive.

[0029] After 5 days of cultivation, the microorganisms were removed and isolated and cultured using the three-zone streak method, which is a known technique and is not specifically limited in this invention.

[0030] The oral microbial isolation and culture device provided in this embodiment has a simple structure, including only a first filter membrane 1, a microporous array chip 2, and a second filter membrane 3. In use, the three components can be connected using silicone, making operation convenient. Then, dental adhesive can be used to fix it inside the oral cavity, thereby culturing oral microorganisms. This device can ensure that the growth of oral microorganisms closely matches the growth conditions of the original microorganisms, without affecting the growth rate of oral microorganisms, and without considering nutrient requirements. This device is highly practical and suitable for large-scale promotion.

[0031] This invention does not specifically limit the material and size of the aforementioned first filter membrane 1, microporous array chip 2, and second filter membrane 3. For example, the microporous array chip 2 is made of polypropylene, which has good mechanical properties and biocompatibility and will not produce toxicity when cultured in the oral cavity. The first filter membrane 1 and the second filter membrane 3 are both made of polycarbonate, and the pore size of the first filter membrane 1 and the second filter membrane 3 is 0.03 μm, which ensures that the first filter membrane 1 and the second filter membrane 3 can effectively isolate the culture micropores 201 for cultivating oral microorganisms and avoid the microorganisms from being affected by the external environment.

[0032] Regarding the size, to avoid causing discomfort to the patient when the device is placed on the buccal side of the patient's maxillary first molar, the microporous array chip 2 has a length of 5-7mm, a width of 3-5mm, and a thickness of 1-2mm, with its four sides transitioned by arcs; the thickness of the first filter membrane 1 and the second filter membrane 3 is 15-20μm.

[0033] The dimensions of the culture micropores 201 in the micropore array chip 2 are described below.

[0034] In one possible implementation, several culture microwells 201 are arranged with different pore sizes, ranging from tens of micrometers to hundreds of micrometers. This range of pore sizes better meets the needs of oral microbial cultivation. Of course, the culture microwells 201 are arranged in an array and are independent of each other. Specifically, the microwell array chip 2 has multiple microwell arrays 202, each containing multiple culture wells. The culture wells within the same microwell array 202 have the same pore size, but the pore sizes of the culture wells in different microwell arrays 202 are different. For example, as... Figure 4 As shown, the number of micropore arrays 202 is set to five, and the pore sizes of the culture wells contained in the five micropore arrays 202 are 8 micrometers, 20 micrometers, 60 micrometers, 180 micrometers and 540 micrometers respectively.

[0035] In another possible implementation, such as Figure 3 As shown, the size of the culture microwell 201 can be set to one size, that is, a microwell array is set. In this case, the inner diameter of the microwell can be set to 400 μm, and the distance between any two culture microwells 201 is 200 μm.

[0036] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An apparatus for isolating and culturing oral microorganisms, characterized in that, It comprises, from bottom to top: a first filter membrane (1), a microporous array chip (2), and a second filter membrane (3); the first filter membrane (1) or the second filter membrane (3) is used to be placed on the patient's teeth; The micropore array chip (2) is provided with a plurality of through culture micropores (201), the first filter membrane (1) covers the bottom of all culture micropores (201), and the second filter membrane (3) covers the top of all culture micropores (201). The culture micropores (201) are used for the growth and reproduction of oral microorganisms.

2. The apparatus for isolating and culturing oral microorganisms according to claim 1, characterized in that, Both the first filter membrane (1) and the second filter membrane (3) are bonded to the microporous array chip (2).

3. The apparatus for isolating and culturing oral microorganisms according to claim 1 or 2, characterized in that, The pore size of both the first filter membrane (1) and the second filter membrane (3) is 0.03 μm.

4. The apparatus for isolating and culturing oral microorganisms according to claim 1, characterized in that, The micropore array chip (2) is provided with a plurality of micropore arrays (202), and each micropore array (202) contains a plurality of culture micropores (201).

5. The apparatus for isolating and culturing oral microorganisms according to claim 4, characterized in that, The culture micropores (201) of different micropore arrays (202) have different pore sizes.

6. The apparatus for isolating and culturing oral microorganisms according to claim 1, characterized in that, The micropore array chip (2) is made of polypropylene.

7. The apparatus for isolating and culturing oral microorganisms according to claim 1 or 6, characterized in that, The micro-hole array chip (2) has a length of 5-7 mm, a width of 3-5 mm, and a thickness of 1-2 mm.

8. The apparatus for isolating and culturing oral microorganisms according to claim 1, characterized in that, The thickness of the first filter membrane (1) and the second filter membrane (3) is 15-20 μm.

9. The apparatus for isolating and culturing oral microorganisms according to claim 8, characterized in that, Both the first filter membrane (1) and the second filter membrane (3) are made of polycarbonate.

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