Thermal cycle amplification module

Abstract

The utility model provides a thermal cycle amplification module. The thermal cycle amplification module comprises a bottom frame, a temperature control assembly, a gland assembly and a driving assembly. The temperature control assembly is provided with a hole groove; the gland assembly is arranged on the upper side of the hole groove and comprises an upper gland, a lower gland, an elastic assembly and a sealing piece. The lower gland is elastically connected with the lower surface of the upper gland through an elastic component; the sealing element is mounted on one side, facing the hole groove, of the lower gland; the driving assembly comprises a driving part, a first connecting rod and a second connecting rod; one end of the first connecting rod is hinged with the upper gland; the other end of the first connecting rod is in transmission connection with the driving piece; one end of the second connecting rod is hinged to the upper gland, the other end of the second connecting rod is hinged to the bottom frame, and the bottom frame, the first connecting rod, the second connecting rod and the upper gland form a parallelogram mechanism. The driving part drives the first connecting rod to rotate relative to the underframe, so that the first connecting rod and the second connecting rod drive the upper gland to lift or fall horizontally relative to the hole groove. According to the thermal cycle amplification module, the amplification experiment process is simplified.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of molecular diagnostics, in particular to a thermal cycling amplification module. BACKGROUND

[0002] Before detecting a specific gene, low-concentration nucleic acid purified by nucleic acid extraction needs to be amplified by a polymerase chain reaction (PCR) to obtain a target template of a certain concentration for detection and research of the target template.

[0003] Current thermal cycling amplification modules are divided into two types: one is a black horse amplification instrument mode, in which the thermal cycling amplification module is made into a separate PCR amplification instrument, and when used, the extracted nucleic acid needs to be manually constructed into a system and then placed in the PCR amplification instrument for amplification, and after amplification, it is manually taken out for subsequent testing; the other is to integrate the amplification module with an opening cover, an air duct system, etc. into a thermal cycling amplification module, which is a key part of an automatic instrument, and can link the entire experimental process of molecular diagnostics before and after processing.

[0004] The existing PCR amplification instrument or thermal cycling amplification module adopts a rotary cover opening mode, and a spring buffer compression mechanism needs to be provided on the cover. The PCR amplification instrument is designed with a compression knob on the cover, and the manual compression knob on the thermal cycling amplification module of the automatic instrument is changed into a step motor driven gear rack vertically moving up and down. When working, the cover is manually or automatically rotated to cover, and the cover clamps the amplification heating area. At this time, the buffer compression mechanism on the cover has not contacted the amplification tube port, and the buffer compression mechanism needs to be manually rotated or the motor driven gear rack to be driven to lower and compress the amplification tube.

[0005] After the experiment is completed, the cover cannot be directly opened, otherwise the tube port of the amplification tube close to the rotary shaft of the cover will be clamped under the action of the reaction force of the spring, which will cause the reagent to be spilled and the instrument to be contaminated. The buffer compression mechanism needs to be manually raised and released by rotating the compression knob or the motor driven gear rack, and then the cover is rotated to open. Therefore, the buffer compression mechanism needs to be pressed down after the cover is closed and raised before the cover is opened in each experiment, which leads to a complex experimental process and low experimental efficiency. CONTENT OF THE INVENTION

[0006] The present application provides a thermal cycling amplification module, which can solve the problems of complex cover closing and opening process and low experimental efficiency.

[0007] To solve the above technical problems, the application provides a thermal cycle amplification module, which comprises a chassis, a temperature control assembly, a gland assembly and a driving assembly. The temperature control assembly is installed on the chassis, and the temperature control assembly is provided with a hole groove for placing an amplification tube and is used for raising and lowering the temperature of the amplification tube. The gland assembly is arranged on the upper side of the hole groove, and the gland assembly comprises an upper gland, a lower gland, an elastic assembly and a sealing element. The lower gland is elastically connected to the lower surface of the upper gland through the elastic assembly. The sealing element is installed on the side of the lower gland facing the hole groove. The driving assembly comprises a driving piece, a first connecting rod and a second connecting rod. One end of the first connecting rod is hinged to the upper gland, and the other end of the first connecting rod is in transmission connection with the driving piece. One end of the second connecting rod is hinged to the upper gland, and the other end of the second connecting rod is hinged to the chassis. The chassis, the first connecting rod, the second connecting rod and the upper gland constitute a parallelogram mechanism. The driving piece is used to drive the first connecting rod to rotate relative to the chassis, so that the first connecting rod and the second connecting rod drive the upper gland to be lifted or lowered in translation relative to the hole groove, so as to switch the opening state and the closed state of the gland assembly. In the closed state, the sealing element can abut against the tube opening of the amplification tube under the action of the elastic assembly, so as to seal the amplification tube.

[0008] In an embodiment, the driving assembly further comprises a driving pulley, a driven pulley, a transmission belt and a rotating shaft. The rotating shaft is in rotational connection with the chassis. The driving piece has an output shaft. The driving pulley is arranged on the output shaft. The driven pulley is arranged on the rotating shaft. The driving pulley and the driven pulley are in transmission connection through the transmission belt. One end of the first connecting rod away from the upper gland is arranged on the output shaft.

[0009] In an embodiment, the number of the first connecting rods is two, which are a first sub-connecting rod and a second sub-connecting rod. The number of the second connecting rods is two, which are a third sub-connecting rod and a fourth sub-connecting rod. The number of the parallelogram mechanisms is two. The first sub-connecting rod, the third sub-connecting rod, the chassis and the upper gland constitute one parallelogram mechanism. The second sub-connecting rod, the fourth sub-connecting rod, the chassis and the upper gland constitute another parallelogram mechanism.

[0010] In an embodiment, the upper gland has a first end and a second end in the axial direction of the output shaft. The first sub-connecting rod is hinged to the first end of the upper gland. The second sub-connecting rod is hinged to the second end of the upper gland. The lower gland is located between the first sub-connecting rod and the second sub-connecting rod. The first sub-connecting rod and the second sub-connecting rod are connected to the two ends of the axial direction of the output shaft, respectively.

[0011] In an embodiment, the elastic assembly comprises a guide piece and a compression spring. One end of the guide piece is fixedly connected to the upper gland. The other end of the guide piece is in sliding connection with the lower gland. One end of the compression spring is connected to the upper gland. The other end of the compression spring is connected to the lower gland.

[0012] In an embodiment, a sliding groove is formed on the sidewall of the lower pressing cover, the sliding groove has an opening on the side facing the hole groove, and a sealing member is slidingly installed in the sliding groove and can abut against the amplification tube through the opening.

[0013] In an embodiment, the hole grooves are multiple, and some of the hole grooves are multi-tube hole grooves, and each multi-tube hole groove is arranged side by side, and the other hole grooves are centrifugal tube hole grooves, and the hole diameter of the centrifugal tube hole grooves is larger than that of the multi-tube hole grooves.

[0014] In an embodiment, the temperature control assembly comprises a heating member and a refrigeration sheet, and the heating member is provided with a hole groove, and the refrigeration sheet is arranged at the bottom of the heating member.

[0015] In an embodiment, the thermal cycle amplification module further comprises a heat conduction plate, a heat dissipation sheet and a fan, the heat conduction plate is connected with the heating member, the heat dissipation sheet is connected to the heat conduction plate, the fan is arranged below the heat dissipation sheet, the air outlet of the fan is arranged opposite to the air inlet of the heat dissipation sheet, and the air outlet of the heat dissipation sheet faces the two sides of the thermal cycle amplification module.

[0016] In an embodiment, the upper pressing cover is provided with a handle or a groove on the side away from the lower pressing cover.

[0017] The thermal cycle amplification module provided by the present application comprises a driving assembly, the first connecting rod, the second connecting rod and the upper pressing cover of the driving assembly can constitute a parallelogram mechanism, when the driving member drives the first connecting rod to rotate relative to the base frame after the experiment is completed, the first connecting rod and the second connecting rod can drive the upper pressing cover to be translated to the obliquely upper side of the amplification tube, and the upper pressing cover is always parallel to the base frame when being lifted, so that the pressing cover assembly will not be stuck to the amplification tube under the action of the elastic assembly, and therefore the thermal cycle amplification module provided by the present application does not need to be provided with a mechanism for lifting the lower pressing cover by a motor driving gear and rack, and only one driving member is needed to drive the pressing cover assembly to open and close the cover, so that the amplification experiment process and the structure of the thermal cycle amplification module are simplified, and the efficiency of the amplification experiment is improved. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The structure schematic diagram of the thermal cycle amplification module provided by an embodiment of the present application in an open state is shown;

[0019] Figure 2 The structure schematic diagram of the thermal cycle amplification module provided by another embodiment of the present application is shown;

[0020] Figure 3 The structure schematic diagram of the temperature control assembly, the heat conduction plate, the heat dissipation sheet and the fan provided by an embodiment of the present application is shown;

[0021] Figure 4 The sectional view of Figure 3 ;

[0022] Figure 5A structure diagram of the thermal cycle amplification module in a closed state according to an embodiment of the present application is shown in FIG. 1.

[0023] Figure 6 A sectional view of the thermal cycle amplification module according to an embodiment of the present application is shown in FIG. 2.

[0024] Figure 7 A structure diagram of the thermal cycle amplification module according to another embodiment of the present application is shown in FIG. 3.

[0025] The reference signs: base frame 10, temperature control assembly 20, multi-union hole groove 21, centrifugal tube hole groove 22, heating element 23, refrigeration sheet 24, gland assembly 30, upper gland 31, groove 311, lower gland 32, sliding groove 321, elastic assembly 33, guide 331, compression spring 332, sealing element 34, driving assembly 40, driving element 41, first connecting rod 42, first sub-connecting rod 421, second sub-connecting rod 422, second connecting rod 43, third sub-connecting rod 431, fourth sub-connecting rod 432, driving pulley 44, driven pulley 45, transmission belt 46, rotating shaft 47, amplification tube 50, heat-conducting plate 60, heat sink 70, fan 80. DETAILED DESCRIPTION

[0026] The present application will be further described in details through specific embodiments in combination with the drawings. In different embodiments, similar elements are denoted by similar reference signs. In the following embodiments, many details are described in order to make the present application better understood. However, those skilled in the art can easily recognize that some features can be omitted in different cases, or can be replaced by other elements, materials, methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application being overwhelmed by too much description, and it is not necessary to describe these related operations in detail for those skilled in the art according to the description in the specification and general technical knowledge in the art.

[0027] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate way to form various embodiments, and the operation steps involved in each embodiment can also be sequentially adjusted or adjusted in a manner that can be easily apparent to those skilled in the art. Therefore, the specification and drawings are only for the purpose of clearly describing one embodiment, and do not mean that the composition and / or order is necessary.

[0028] The serial numbers of the components in this paper, such as "first", "second", etc., are only used to distinguish the described objects, and do not have any order or technical meaning. The "connection" and "coupling" in the present application include direct and indirect connections (couplings) unless otherwise specified.

[0029] The terms "parallel", "perpendicular" and the like are defined in relation to the current process level, rather than the absolute strict definition in mathematics, and a small deviation is allowed, and approximate parallel, approximate perpendicular and the like are also allowed. For example, A is parallel to B, which means that A is parallel to B or approximately parallel to B, and the included angle between A and B is between 0° and 10°. For example, A is perpendicular to B, which means that A is perpendicular to B or approximately perpendicular to B, and the included angle between A and B is between 80° and 100°. The orientation terms mentioned in the embodiments of the present application, such as "upper", "inner", "outer", "side" and the like, are only the directions of the drawings, therefore, the orientation terms are used to better and more clearly illustrate and understand the embodiments of the present application, and are not intended to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0030] As shown in Figure 1 , the present application provides a thermal cycle amplification module, which can obtain a certain concentration of target template by PCR amplification of low-concentration nucleic acid purified by nucleic acid extraction. The thermal cycle amplification module of the present application can be applied to automatic instrument integration, and can be connected to the front and rear processing modules of the automatic instrument to complete the entire experimental process of molecular diagnosis, wherein the amplification flux of the thermal cycle amplification module can be determined according to the flux of the automatic instrument.

[0031] Specifically, as shown in Figure 1 and Figure 2 , the thermal cycle amplification module comprises a chassis 10, a temperature control assembly 20, a gland assembly 30 and a driving assembly 40. The chassis 10 is a support frame of the entire thermal cycle amplification module, and the temperature control assembly 20 and the driving assembly 40 are both mounted on the chassis 10. As shown in Figure 3 , the temperature control assembly 20 is provided with a hole slot, and the hole slot is used to place an amplification tube 50 (i.e. a PCR tube).

[0032] In an embodiment, as shown in Figure 1 and Figure 3 , the temperature control assembly 20 can be compatible with a single centrifugal tube and a multi-tube (for example, an eight-tube), and the number of hole slots is multiple, wherein a part of the hole slots are multi-tube hole slots 21, and the other part of the hole slots are centrifugal tube hole slots 22. The hole diameter of the centrifugal tube hole slots 22 is larger than the hole diameter of the multi-tube hole slots 21. Each multi-tube hole slot 21 is arranged side by side, and multiple rows of multi-tube hole slots 21 can also be arranged side by side, so that each multi-tube hole slot 21 is arranged in an array. For example, in the embodiments of Figure 1 and Figure 3 , four rows of eight-tube hole slots are provided. The hole slots of the centrifugal tube can also be arranged side by side and in an array, for example, in the embodiments of Figure 1 and Figure 2, the array is provided with 3 rows of 7 columns of centrifugal tube slots 22, and each row of centrifugal tube slots 22 is arranged between two adjacent rows of multi-tube slots 21. Each centrifugal tube slot 22 is arranged at the center of four rectangular multi-tube slots 21, so that the multi-tube slots 21 and the centrifugal tube slots 22 are staggered, so that the temperature control assembly 20 can accommodate more slots.

[0033] As shown in Figure 4 , the temperature control assembly 20 is used to raise and lower the temperature of the amplification tube 50. In one embodiment, the temperature control assembly 20 includes a heating element 23 and a cooling fin 24. The upper part of the heating element 23 is provided with a slot, and the cooling fin 24 is arranged at the bottom of the heating element 23. The heating element 23 can heat the PCR tube, and the cooling fin 24 can lower the temperature of the PCR tube to meet the requirement of precise temperature control in PCR amplification.

[0034] In one embodiment, as shown in Figures 1-4 , the thermal cycling amplification module further includes a heat conduction plate 60, a heat sink 70 and a fan 80. The heat conduction plate 60 is connected with the heating element 23, the heat sink 70 is connected to the heat conduction plate 60, and the fan 80 is arranged below the heat sink 70. The heat conduction plate 60 can quickly transfer heat and guide the heat of the heating element 23 to the heat sink 70 to enhance the thermal cycling. The heat sink 70 can increase the heat dissipation area and improve the heat dissipation efficiency, quickly spread the heat of the heat conduction plate 60 on the heat sink 70, and take away the heat through air convection. The fan 80 is an air inlet fan that can accelerate air convection. The air outlet of the fan 80 is arranged opposite to the air inlet of the heat sink 70, and the air outlet of the heat sink 70 faces the two sides of the thermal cycling amplification module. That is, the air inlet of the thermal cycling amplification module of the present application is arranged at the bottom, and the air blows in from bottom to top, which can ensure that the air blowing into the heat sink 70 is uniform, and the air outlet of the heat sink 70 blows out from the two sides of the heat sink 70. In the automatic instrument, the heating element 23 is located on the platform surface of the automatic instrument. The entire automatic instrument performs experimental operation on the thermal cycling amplification module and other modules on the platform surface. By arranging the thermal cycling system at the bottom of the thermal cycling amplification module, i.e. below the platform surface, it can be ensured that the air inlet and outlet of the thermal cycling system will not affect the experimental operation, and the air outlet and air inlet face different directions and are independent of each other.

[0035] As shown in Figure 1 and Figure 5 , the gland assembly 30 has an open state and a closed state, Figure 1 , the open state of the gland assembly 30, Figure 5 , the closed state of the gland assembly 30. The gland assembly 30 is arranged on the upper side of the slot. It should be noted that the upper side of the slot does not mean only the upper side of the slot. As long as the height of the gland assembly 30 is higher than the height of the slot, the gland assembly 30 is arranged on the upper side of the slot. For example, as shown in Figure 5In the embodiment, the gland assembly 30 is arranged on the upper side of the hole groove. Figure 1 In the embodiment, the gland assembly 30 is arranged on the upper side of the hole groove.

[0036] As shown in Figure 6 and Figure 7 , the gland assembly 30 comprises an upper gland 31, a lower gland 32, an elastic assembly 33 and a sealing member 34. The lower gland 32 is elastically connected with the lower surface of the upper gland 31 through the elastic assembly 33. Specifically, in an embodiment, the elastic assembly 33 comprises a guide piece 331 and a compression spring 332. One end of the guide piece 331 is fixedly connected with the upper gland 31, for example, the guide piece 331 can be threadedly connected with the upper gland 31. The other end of the guide piece 331 is slidably connected with the lower gland 32. The end of the guide piece 331 away from the upper gland 31 is provided with a limiting structure. The lower gland 32 can be provided with a through hole, and the guide piece 331 is arranged in the through hole. The lower gland 32 is located between the limiting structure and the upper gland 31, that is, the limiting structure and the upper gland 31 are used for limiting the vertical direction of the lower gland 32. One end of the compression spring 332 is connected with the upper gland 31, and the other end of the compression spring 332 is connected with the lower gland 32. The sealing member 34 is installed on the side of the lower gland 32 facing the hole groove. Specifically, in an embodiment, as shown in Figure 7 , a sliding groove 321 is formed in the side wall of the lower gland 32. The side of the sliding groove 321 facing the hole groove is provided with an opening. The sealing member 34 is slidably installed in the sliding groove 321, and the sealing member 34 can abut against the amplification tube 50 through the opening. By arranging the sealing member 34 and the sliding connection of the lower gland 32, the sealing member 34 can be easily taken out and cleaned after the experiment.

[0037] When the driving assembly 40 drives the gland assembly 30 to be pressed, the sealing member 34 covers the tube opening of the amplification tube 50. The gland assembly 30 is further pressed. At this time, since the sealing member 34 has abutted against the amplification tube 50, the sealing member 34 and the lower gland 32 cannot continue to descend. The upper gland 31 is further pressed under the action of the driving assembly 40. The upper gland 31 is relatively close to the lower gland 32. The compression spring 332 is further compressed. The elastic force of the compression spring 332 acts on the lower gland 32, so that the sealing member 34 tightly presses the tube opening of the amplification tube 50, so as to realize good sealing of the amplification tube 50 and prevent aerosol from volatilizing. In addition, the elastic member can tightly contact the amplification tube 50 with the heating member 23, so as to improve the heat conduction speed.

[0038] As shown in Figure 1 and Figure 2As shown, the driving assembly 40 comprises a driving member 41, a first connecting rod 42 and a second connecting rod 43. The driving member 41 is mounted on the chassis 10, one end of the first connecting rod 42 is hinged to the upper cover 31, and the other end of the first connecting rod 42 is in transmission connection with the driving member 41. One end of the second connecting rod 43 is hinged to the upper cover 31, and the other end of the second connecting rod 43 is hinged to the chassis 10. The chassis 10, the first connecting rod 42, the second connecting rod 43 and the upper cover 31 constitute a parallelogram mechanism. The driving member 41 is used to drive the first connecting rod 42 to rotate relative to the chassis 10, so as to drive the first connecting rod 42 and the second connecting rod 43 to lift or lower the upper cover 31 in translation relative to the hole groove, so as to switch the opening state and the closing state of the cover assembly 30. In the opening state, the upper cover 31 is located obliquely above the hole groove, so that the hole groove is directly above the hole groove, so as to facilitate the pipette arm above the hole groove to operate above the hole groove. In the closing state, the sealing member 34 can abut against the pipe opening of the amplification tube 50 under the action of the elastic assembly 33, so as to seal the amplification tube 50. Among them, the opening and closing of the cover assembly 30 cannot occupy too much space, cannot interfere with the movement track of the pipette arm, and also cannot interfere with the lowering of the pipette of the pipette arm.

[0039] The existing thermal cycle amplification module is controlled to open the flip cover in rotation. If a driving mechanism for lifting the spring buffer compression mechanism for controlling the flip cover is not set, the pipe opening of the amplification tube 50 close to the rotation axis of the flip cover will be clamped under the elastic action of the spring buffer compression mechanism on the flip cover when the flip cover is opened. Setting the driving mechanism for lifting the spring buffer compression mechanism will result in too many driving elements, the structure of the thermal cycle amplification module is complex, and the buffer compression mechanism needs to be pressed down after the cover is closed and lifted before the cover is opened in each experiment, which results in a complex experimental process and low experimental efficiency.

[0040] The thermal cycle amplification module of the present application sets the driving assembly 40, the first connecting rod 42 and the second connecting rod 43 of the driving assembly 40 and the upper pressing cover 31 can constitute a parallelogram mechanism, when the driving part 41 drives the first connecting rod 42 to rotate relative to the base frame 10, the first connecting rod 42 and the second connecting rod 43 can drive the upper pressing cover 31 to be lifted in a translational manner to the obliquely upper side of the amplification tube 50, the upper pressing cover 31 is always parallel to the base frame 10 when being lifted, thus the pressing cover assembly 30 cannot be stuck to the amplification tube 50 under the action of the elastic assembly 33, thus the thermal cycle amplification module of the present application does not need to set the motor driving gear rack to lift the lower pressing cover 32, only one driving part 41 is needed to drive the pressing cover assembly 30 to open and close the cover, which simplifies the structure of the thermal cycle amplification module, and also cannot cause the problem that the amplification tube 50 is stuck and the reagent is spilled when the cover is opened, the process of setting the motor driving gear rack to lift and lower the lower pressing cover 32 is saved, thus the amplification experiment process is simplified and the efficiency of the amplification experiment is improved. And the pressing cover assembly 30 always moves in parallel, thus the amplification tube 50 can be kept under uniform pressing force, the airtightness and the heat preservation are improved.

[0041] The process of using the thermal cycle amplification module is as follows: 1. The driving part 41 is started to drive the first connecting rod 42 and the second connecting rod 43 to rotate, so as to drive the pressing cover assembly 30 to be opened in a translational manner to the upper side of the hole groove; 2. The sealing piece 34 is installed on the lower pressing cover 32; 3. The amplification tube 50 is placed into the hole groove; 4. After the automatic instrument is started and the nucleic acid extraction work is completed, the purified nucleic acid is automatically transferred into the amplification tube; 5. After the amplification tube 50 is completed, the driving part 41 is started to drive the pressing cover assembly 30 to be switched to a closed state, the pressing cover assembly 30 presses the amplification tube 50, and the sealing piece 34 seals the tube opening. 6. The amplification tube 50 is periodically heated and cooled according to the PCR amplification temperature requirement until the amplification is completed. 7. The driving part 41 drives the upper pressing cover 31 mechanism to be switched to an open state, the pipetting arm quantitatively takes out the amplified product through the pipettor and transfers it to the subsequent hybridization platform for subsequent testing; 8. After all the experiments are completed, the amplification tube 50 is manually taken out, the sealing piece 34 is taken out for cleaning, and the pressing cover assembly 30 is cleaned by spraying alcohol.

[0042] In one embodiment, as Figure 2As shown, the driving assembly 40 further comprises a driving pulley 44, a driven pulley 45, a transmission belt 46 and a rotating shaft 47, the rotating shaft 47 is rotatably connected with the chassis 10, the driving member 41 has an output shaft, the driving pulley 44 is arranged on the output shaft, the driven pulley 45 is arranged on the rotating shaft 47, and the driving pulley 44 is in driving connection with the driven pulley 45 through the transmission belt 46. The end of the first connecting rod 42 away from the upper pressing cover 31 is arranged on the output shaft. In this way, the driving member 41 can drive the driving pulley 44 to rotate, the driving pulley 44 drives the driven pulley 45 to rotate through the transmission belt 46, and the output shaft rotates synchronously with the driven pulley 45 to drive the first connecting rod 42 to rotate, so that the transmission is more stable through the belt transmission pair.

[0043] In an embodiment, as shown in Figure 7 The number of the first connecting rods 42 is two, which are respectively a first sub-connecting rod 421 and a second sub-connecting rod 422, and the number of the second connecting rods 43 is two, which are respectively a third sub-connecting rod 431 and a fourth sub-connecting rod 432. The number of the parallelogram mechanisms is two. The first sub-connecting rod 421, the third sub-connecting rod 431, the chassis 10 and the upper pressing cover 31 constitute a parallelogram mechanism. The first sub-connecting rod 421, the fourth sub-connecting rod 432, the chassis 10 and the upper pressing cover 31 constitute another parallelogram mechanism. The upper pressing cover 31 has a first end and a second end along the axial direction of the output shaft, the first sub-connecting rod 421 is hinged to the first end of the upper pressing cover 31, the first sub-connecting rod 421 is hinged to the second end of the upper pressing cover 31, and the lower pressing cover 32 is located between the first sub-connecting rod 421 and the second sub-connecting rod 422; the first sub-connecting rod 421 and the second sub-connecting rod 422 are respectively connected with the two ends of the axial direction of the output shaft. Through the above structure, the translation movement of the pressing cover assembly 30 driven by the connecting rods is more stable.

[0044] In an embodiment, as shown in Figure 1 In addition, in an embodiment, a heating element can be arranged on the sealing member 34, so that the sealing member 34 has a heating function to increase the accuracy of temperature control.

[0045] The above application of specific examples to illustrate the present application, is only used to help understand the present application, and does not limit the present application. For those skilled in the art to which the present application belongs, according to the idea of the present application, a number of simple deductions, deformations or substitutions can be made.

Claims

1. A thermal cycling amplification module, characterized in that, include: Base frame; A temperature control component is mounted on the base frame. The temperature control component has slots for placing amplification tubes and is used to raise and lower the temperature of the amplification tubes. A cap assembly is disposed on the upper side of the slot. The cap assembly includes an upper cap, a lower cap, an elastic component, and a seal. The lower cap is elastically connected to the lower surface of the upper cap through the elastic component. The seal is installed on the side of the lower cap facing the slot. A drive assembly includes a drive member, a first connecting rod, and a second connecting rod; one end of the first connecting rod is hinged to the upper pressure cover, and the other end of the first connecting rod is connected to the drive member; one end of the second connecting rod is hinged to the upper pressure cover, and the other end of the second connecting rod is hinged to the base frame; the base frame, the first connecting rod, the second connecting rod, and the upper pressure cover constitute a parallelogram mechanism. The driving component is used to drive the first connecting rod to rotate relative to the base frame, so that the first connecting rod and the second connecting rod drive the upper pressure cap to be lifted or lowered relative to the slot, thereby switching the open and closed states of the pressure cap assembly; in the closed state, the sealing component can press against the opening of the amplification tube under the action of the elastic component to seal the amplification tube.

2. The thermal cycling amplification module according to claim 1, characterized in that, The drive assembly further includes a drive pulley, a driven pulley, a transmission belt, and a rotating shaft. The rotating shaft is rotatably connected to the base frame. The drive component has an output shaft. The drive pulley is located on the output shaft, and the driven pulley is located on the rotating shaft. The drive pulley and the driven pulley are connected by the transmission belt. The end of the first connecting rod away from the upper pressure cover is located on the output shaft.

3. The thermal cycling amplification module according to claim 2, characterized in that, The number of first connecting rods is two, namely the first sub-connecting rod and the second sub-connecting rod; the number of second connecting rods is two, namely the third sub-connecting rod and the fourth sub-connecting rod; the number of parallelogram mechanisms is two; the first sub-connecting rod, the third sub-connecting rod, the base frame, and the upper pressure cover constitute one parallelogram mechanism; the second sub-connecting rod, the fourth sub-connecting rod, the base frame, and the upper pressure cover constitute another parallelogram mechanism.

4. The thermal cycling amplification module according to claim 3, characterized in that, The upper pressure cover has a first end and a second end along the axial direction of the output shaft. The first sub-connecting rod is hinged to the first end of the upper pressure cover, and the second sub-connecting rod is hinged to the second end of the upper pressure cover. The lower pressure cover is located between the first sub-connecting rod and the second sub-connecting rod. The first sub-connecting rod and the second sub-connecting rod are respectively connected to the two ends of the output shaft in the axial direction.

5. The thermal cycling amplification module according to any one of claims 1-4, characterized in that, The elastic component includes a guide and a compression spring. One end of the guide is fixedly connected to the upper pressure cover, and the other end of the guide is slidably connected to the lower pressure cover. One end of the compression spring is connected to the upper pressure cover, and the other end of the compression spring is connected to the lower pressure cover.

6. The thermal cycling amplification module according to any one of claims 1-4, characterized in that, A sliding groove is provided on the side wall of the lower pressure cap, and the sliding groove has an opening on the side facing the slot. The sealing element is slidably installed in the sliding groove, and the sealing element can press against the amplification tube through the opening.

7. The thermal cycling amplification module according to any one of claims 1-4, characterized in that, The number of slots is multiple, some of which are multi-pipe slots and others are centrifuge tube slots; the multi-pipe slots are arranged side by side, and the diameter of the centrifuge tube slots is larger than the diameter of the multi-pipe slots.

8. The thermal cycling amplification module according to any one of claims 1-4, characterized in that, The temperature control component includes a heating element and a cooling element. The heating element has the groove, and the cooling element is disposed at the bottom of the heating element.

9. The thermal cycling amplification module according to claim 8, characterized in that, It also includes a heat-conducting plate, a heat sink, and a fan. The heat-conducting plate is connected to the heating element, the heat sink is connected to the heat-conducting plate, the fan is located below the heat sink, the air outlet of the fan is opposite to the air inlet of the heat sink, and the air outlet of the heat sink faces both sides of the thermal cycling amplification module.

10. The thermal cycling amplification module according to any one of claims 1-4, characterized in that, The upper pressure cover has a handle or groove on the side opposite to the lower pressure cover.

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