Cell culture dish for single cells

Abstract

The utility model relates to the technical field of biological experiment instrument consumables, in particular to a cell culture dish for single cells, which comprises a placing frame, a culture dish body is mounted on the placing frame through a fixing structure, a plurality of grooves are formed in the placing frame at equal intervals, the culture dish body is clamped in the grooves, two bumps are fixedly connected onto the culture dish body, and the bumps are fixed on the placing frame. A plurality of clamping grooves are formed in the containing frame, the protruding blocks are clamped in the clamping grooves, a sliding block is connected to the containing frame in a sliding mode, a rotating shaft is fixedly connected to the sliding block, a fixing plate is rotationally connected to the rotating shaft, the fixing plate abuts against the protruding blocks, the rotating shaft is sleeved with a torsional spring, one end of the torsional spring is fixedly connected with the sliding block, and the other end of the torsional spring is fixedly connected with the fixing plate. A limiting structure is mounted on the fixing plate, and the culture dish body is covered with a dish cover; a plurality of one-hole cell culture dishes are placed in the placing frame, and then the culture dish body is fixed by using the fixing structure, so that the culture dish body is stably placed.

Description

Technical Field

[0001] This utility model relates to a cell culture dish, specifically a cell culture dish for single cells, and belongs to the field of biological experimental instrument consumables technology. Background Technology

[0002] A cell culture dish is a vessel used for cell culture, usually made of glass or plastic. When culturing single cells, a cell culture dish provides a closed, sterile environment, allowing cells to grow and multiply under specific culture conditions. Cell culture dishes usually have a transparent bottom, making it easy to observe the state and growth of cells. Among existing cell culture dishes, a single-well cell culture dish provides a large culture area and a relatively independent culture environment, making it suitable for single-cell or small-scale cell culture experiments.

[0003] However, after the existing single-well cell culture dishes are seeded with cell suspension, they are usually stacked directly in the incubator because the dishes lack a fixing component. This direct stacking method makes the dishes unstable, and when one dish is removed, the others are prone to slipping, causing the cell suspension inside the dish to shake or spill, which in turn affects the accuracy of the experimental data of cell culture. Utility Model Content

[0004] The purpose of this invention is to provide a cell culture dish for single cells in order to solve the above problems. By placing multiple one-well cell culture dishes in a placement frame and then fixing the culture dish body with a fixing structure, the culture dish body is placed stably. Moreover, the culture dish body in the placement frame consists of multiple individual culture dish bodies, which can be installed arbitrarily in the placement frame according to actual needs. Compared with multi-well cell culture dishes, it is more flexible and avoids resource waste.

[0005] This utility model achieves the above-mentioned objective through the following technical solution: a cell culture dish for single cells includes a placement frame, on which a culture dish body is mounted by a fixing structure. The fixing structure includes grooves, and multiple grooves are equidistantly arranged on the placement frame. The culture dish body is engaged in the grooves. Two protrusions are fixedly connected to the culture dish body. Multiple slots are provided on the placement frame, and the protrusions are engaged in the slots. A slider is slidably connected to the placement frame, and a rotating shaft is fixedly connected to the slider. A fixing plate is rotatably connected to the rotating shaft, and the fixing plate abuts against the protrusions. A torsion spring is sleeved on the rotating shaft, one end of which is fixedly connected to the slider, and the other end of which is fixedly connected to the fixing plate. A limit structure is installed on the fixing plate, and a dish lid is placed on the culture dish body.

[0006] Preferably, the slot and the groove are connected, two adjacent protrusions are symmetrically arranged, the cross-sections of the protrusions and the slot are both trapezoidal, a screw is rotatably connected to the placement frame, and the slider is threadedly connected to the screw.

[0007] Preferably, the limiting structure includes a limiting block, the limiting block is slidably connected to the fixing plate, the placing frame has a limiting groove, and the limiting block is engaged with the limiting groove.

[0008] Preferably, a spring is installed between the fixing plate and the limiting block, with one end of the spring fixedly connected to the fixing plate and the other end of the spring fixedly connected to the limiting block.

[0009] Preferably, a guide rod is fixedly connected to the fixing plate, and the limiting block is slidably connected to the guide rod.

[0010] Preferably, the end of the limiting block has an inclined structure, and the cross-section of the guide rod has a "T" shape.

[0011] Preferably, the outer wall of the culture dish body is provided with a graduation strip, and a rubber pad is installed at the bottom of the culture dish body.

[0012] Preferably, an anti-slip sleeve is fixedly connected to the outer wall of the culture dish body, and the cross-section of the anti-slip sleeve has a serrated structure.

[0013] Preferably, the bottom end of the placement frame is fixedly connected with four positioning posts, and the upper end face of the placement frame is provided with four positioning holes, and the positioning posts are inserted into the positioning holes on the adjacent placement frames.

[0014] The beneficial effects of this utility model are as follows: A culture dish body is mounted on the placement frame via a fixing structure. Multiple grooves are equidistantly spaced on the placement frame, and the culture dish body engages with these grooves. Two protrusions are fixedly connected to the culture dish body. Multiple slots are provided on the placement frame, and the protrusions engage with these slots. A slider is slidably connected to the placement frame, and a rotating shaft is fixedly connected to the slider. A fixing plate is rotatably connected to the rotating shaft, and the fixing plate abuts against the protrusions. A torsion spring is sleeved on the rotating shaft, with one end fixedly connected to the slider and the other end fixedly connected to the fixing plate. By placing multiple one-well cell culture dishes within the placement frame and then fixing the culture dish body using the fixing structure, the culture dish body is placed stably. Furthermore, the culture dish body within the placement frame consists of multiple individual culture dish bodies, which can be arbitrarily installed within the placement frame according to actual needs. Compared to multi-well cell culture dishes, this provides greater flexibility and avoids resource waste. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 for Figure 1 The diagram shown is an enlarged view of the structure of part A.

[0017] Figure 3 This is a schematic diagram of the connection structure between the placement frame and the petri dish body of this utility model;

[0018] Figure 4 for Figure 3 The diagram shown is an enlarged view of the structure of section B.

[0019] Figure 5 This is a schematic diagram of the connection structure between the placement frame and the positioning post of this utility model;

[0020] Figure 6 for Figure 5 The diagram shows an enlarged view of section C.

[0021] Figure 7 This is a schematic diagram of the connection structure between the petri dish body and the dish lid of this utility model.

[0022] In the diagram: 1. Placement frame; 2. Fixing structure; 201. Groove; 202. Rotating shaft; 203. Fixing plate; 204. Torsion spring; 205. Protrusion; 206. Slot; 207. Slider; 208. Screw; 3. Petri dish body; 4. Lid; 5. Graduated strip; 6. Anti-slip sleeve; 7. Limiting structure; 701. Limiting block; 702. Limiting groove; 703. Spring; 704. Guide rod; 8. Positioning hole; 9. Positioning post; 10. Rubber pad. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-7As shown, a cell culture dish for single cells includes a placement frame 1. A culture dish body 3 is mounted on the placement frame 1 via a fixing structure 2. The fixing structure 2 includes grooves 201. Multiple grooves 201 are equidistantly arranged on the placement frame 1. The culture dish body 3 engages with the grooves 201. Two protrusions 205 are fixedly connected to the culture dish body 3. Multiple slots 206 are provided on the placement frame 1, and the protrusions 205 engage with the slots 206. A slider 207 is slidably connected to the placement frame 1. A rotating shaft 202 is fixedly connected to the slider 207, and a fixing plate is rotatably connected to the rotating shaft 202. 203, the fixing plate 203 abuts against the protrusion 205, the rotating shaft 202 is fitted with a torsion spring 204, one end of the torsion spring 204 is fixedly connected to the slider 207, the other end of the torsion spring 204 is fixedly connected to the fixing plate 203, the slot 206 communicates with the groove 201, two adjacent protrusions 205 are symmetrically arranged, the cross sections of the protrusions 205 and the slot 206 are both trapezoidal, the placement frame 1 is rotatably connected with a screw 208, the slider 207 is threadedly connected to the screw 208, the fixing plate 203 is equipped with a limiting structure 7, and the petri dish body 3 is covered with a dish lid 4.

[0025] As a technical optimization of this utility model, the limiting structure 7 includes a limiting block 701. The limiting block 701 is slidably connected to the fixing plate 203. A limiting groove 702 is formed on the placement frame 1. The limiting block 701 is engaged with the limiting groove 702. A spring 703 is installed between the fixing plate 203 and the limiting block 701. One end of the spring 703 is fixedly connected to the fixing plate 203, and the other end of the spring 703 is fixedly connected to the limiting block 701. When the fixing plate 203 rotates away from the end of the slider 207 to... When the other side of the placement frame 1 is placed, the inner wall of the placement frame 1 slides against the limiting block 701 toward the inside of the fixing plate 203. At the same time, the limiting block 701 drives the spring 703 to retract. When the limiting block 701 is aligned with the limiting groove 702 on the placement frame 1, the spring 703 resets and drives the limiting block 701 to engage with the limiting groove 702, thereby quickly limiting the fixing plate 203 and preventing the torsion spring 204 from resetting and driving the fixing plate 203 to spring away from the protrusion 205, thus avoiding affecting the fixing function of the fixing plate 203 on the culture dish body 3.

[0026] As a technical optimization of this utility model, a guide rod 704 is fixedly connected to the fixing plate 203, and the limiting block 701 is slidably connected to the guide rod 704. The end of the limiting block 701 has an inclined structure, and the cross-section of the guide rod 704 has a "T" shape. The guide rod 704 has a good guiding effect on the limiting block 701, and the "T" shape of the guide rod 704 can prevent the limiting block 701 from slipping off the fixing plate 203, thereby increasing the stability of the limiting block 701.

[0027] As a technical optimization of this utility model, the outer wall of the culture dish body 3 is provided with a scale strip 5, and a rubber pad 10 is installed at the bottom of the culture dish body 3. When the culture dish body 3 is placed on the operating table, the rubber pad 10 installed at the bottom of the culture dish body 3 can increase the friction between the culture dish body 3 and the operating table, thereby making the culture dish body 3 stable. Then, according to the scale strip 5, an appropriate amount of cell suspension is inoculated into the culture dish body 3. The setting of the scale strip 5 makes it easy to control the amount of cell suspension inoculated into the culture dish body 3.

[0028] As a technical optimization of this utility model, the outer wall of the petri dish body 3 is fixedly connected with an anti-slip sleeve 6, and the cross-section of the anti-slip sleeve 6 is a sawtooth structure; hold the anti-slip sleeve 6 with your hand, and then lift the petri dish body 3 and place it in the groove 201 on the placement frame 1. The anti-slip sleeve 6 makes it more convenient for operators to pick up the petri dish body 3 after wearing gloves, and has a good anti-slip effect. In addition, the anti-slip sleeve 6 is made of transparent material, so it does not affect the staff to see the scale strip 5.

[0029] As a technical optimization of this utility model, four positioning posts 8 are fixedly connected to the bottom end of the placement frame 1, and four positioning holes 9 are opened on the upper end face of the placement frame 1. The positioning posts 8 are inserted into the positioning holes 9 on the adjacent placement frames 1. By inserting the positioning posts 8 on the placement frame 1 into the positioning holes 9 on the adjacent placement frames 1, the placement frames 1 can be stacked, thereby facilitating the effective use of the cultivation space in the incubator.

[0030] In use, the culture dish body 3 is first placed on the operating table. Because a rubber pad 10 is installed at the bottom of the culture dish body 3, the friction between the culture dish body 3 and the operating table is increased, thus ensuring the culture dish body 3 is placed stably. Then, an appropriate amount of cell suspension is inoculated into the culture dish body 3 according to the graduation strip 5. After inoculation, the dish lid 4 is placed on the culture dish body 3 to create a good seal. Holding the anti-slip sleeve 6, the culture dish body 3 is then lifted and placed in the groove 201 on the placement frame 1. The anti-slip sleeve 6 makes it easier for operators to handle the culture dish body 3 while wearing gloves, providing good anti-slip properties. Furthermore, the anti-slip sleeve 6 is made of transparent material, so it does not obstruct the operator's view of the graduation strip. Step 5; Place the inoculated culture dish bodies 3 one by one into the multiple grooves 201 on the placement frame 1. The culture dish body 3 drives the protrusion 205 to engage with the slot 206. After all the culture dish bodies 3 are placed, rotate the fixing plate 203 towards the limiting groove 702. The rotation of the fixing plate 203 drives the torsion spring 204 to twist and store force. Continue to rotate the fixing plate 203 until it is directly above the culture dish body 3. Then, use a hexagonal wrench to turn the screw 208. The screw 208 thread drives the slider 207 to slide downward. The slider 207 slides downward and drives the fixing plate 203 to slide downward through the rotating shaft 202. The downward sliding of the fixing plate 203 also drives the slider 207 on the other side of the placement frame 1 to slide downward. Sliding: Because the diameter of the through hole on the fixing plate 203 is larger than the diameter of the dish lid 4, the fixing plate 203 can penetrate the culture dish body 3 and make the fixing plate 203 press against the protrusion 205 downwards, thus preventing the protrusion 205 from sliding out of the slot 206. The protrusion 205 not only prevents the culture dish body 3 from rotating in the placement frame 1, but also limits the culture dish body 3 by the contact between the fixing plate 203 and the protrusion 205, preventing the culture dish body 3 from sliding out of the placement frame 1. When the end of the fixing plate 203 away from the slider 207 rotates to the other side of the placement frame 1, the inner wall of the placement frame 1 contacts the limiting block 701 and slides towards the inside of the fixing plate 203. At the same time, the limiting block 701 drives the spring 703 to retract. When the limiting block 701 and the placement frame 1... When the limiting groove 702 is aligned, the spring 703 resets and drives the limiting block 701 to engage with the limiting groove 702, thereby quickly limiting the fixing plate 203 and preventing the torsion spring 204 from resetting and driving the fixing plate 203 to spring away from the protrusion 205, thus avoiding affecting the fixing function of the fixing plate 203 on the culture dish body 3; the guide rod 704 has a good guiding effect on the limiting block 701, and the cross section of the guide rod 704 is in the shape of a "T" which can prevent the limiting block 701 from slipping off the fixing plate 203, thereby increasing the stability of the limiting block 701; by inserting the positioning post 8 on the placement frame 1 into the positioning hole 9 on the adjacent placement frame 1, the placement frames 1 can be stacked, thereby facilitating the effective use of the cultivation space in the incubator.

[0031] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0032] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A cell culture dish for single cells, comprising a placement frame (1), characterized in that: The placement frame (1) is fitted with a petri dish body (3) via a fixing structure (2). The fixing structure (2) includes a groove (201). Multiple grooves (201) are equidistantly provided on the placement frame (1). The petri dish body (3) is engaged in the grooves (201). Two protrusions (205) are fixedly connected to the petri dish body (3). Multiple slots (206) are provided on the placement frame (1). The protrusions (205) are engaged in the slots (206). A slider (207) is slidably connected to the placement frame (1). A rotating shaft (202) is fixedly connected to the slider (207), and a fixed plate (203) is rotatably connected to the rotating shaft (202). The fixed plate (203) abuts against the protrusion (205). A torsion spring (204) is sleeved on the rotating shaft (202). One end of the torsion spring (204) is fixedly connected to the slider (207), and the other end of the torsion spring (204) is fixedly connected to the fixed plate (203). A limiting structure (7) is installed on the fixed plate (203). A lid (4) is placed on the petri dish body (3). The limiting structure (7) includes a limiting block (701), which is slidably connected to the fixing plate (203). A limiting groove (702) is opened on the placement frame (1), and the limiting block (701) is engaged in the limiting groove (702). A spring (703) is installed between the fixing plate (203) and the limiting block (701). One end of the spring (703) is fixedly connected to the fixing plate (203), and the other end of the spring (703) is fixedly connected to the limiting block (701). A guide rod (704) is fixedly connected to the fixing plate (203), and the limiting block (701) is slidably connected to the guide rod (704).

2. The cell culture dish for single cells according to claim 1, characterized in that: The slot (206) is connected to the groove (201), and the two adjacent protrusions (205) are symmetrically arranged. The cross-sections of the protrusions (205) and the slot (206) are both trapezoidal. A screw (208) is rotatably connected to the placement frame (1), and the slider (207) is threadedly connected to the screw (208).

3. A cell culture dish for single cells according to claim 1, characterized in that: The end of the limiting block (701) has a beveled structure, and the cross section of the guide rod (704) has a "T" shaped structure.

4. A cell culture dish for single cells according to claim 1, characterized in that: The outer wall of the petri dish body (3) is provided with a scale strip (5), and a rubber pad (10) is installed at the bottom of the petri dish body (3).

5. A cell culture dish for single cells according to claim 1, characterized in that: The outer wall of the culture dish body (3) is fixedly connected with an anti-slip sleeve (6), and the cross section of the anti-slip sleeve (6) is serrated.

6. A cell culture dish for single cells according to claim 1, characterized in that: The bottom end of the placement frame (1) is fixedly connected with four positioning posts (8), and the upper surface of the placement frame (1) is provided with four positioning holes (9). The positioning posts (8) are inserted into the positioning holes (9) on the adjacent placement frame (1).

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