Grinding rod for extracting micro tissue nucleic acid
By designing a transparent grinding rod adapted to PCR tubes, the problems of large size, strong adsorption and opacity of existing grinding rods have been solved, realizing efficient grinding of micro-sized tissues and low-loss nucleic acid extraction, which is suitable for library construction and genotype identification.
Patent Information
- Application Number
- CN202520343928.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-02-13
- Estimated Expiration
- 2035-02-28
AI Technical Summary
Existing grinding rods are too large, have strong material adsorption and are opaque, resulting in insufficient grinding of micro-tissues, high risk of nucleic acid degradation and difficulty in observation.
A transparent grinding rod adapted to PCR tubes was designed. The grinding tip is coated with a low-adsorption coating and is made of transparent polypropylene material. The diameter of the grinding rod is smaller than that of the grinding tip, which facilitates observation and reduces sample loss.
It achieves thorough grinding of trace tissues, reduces the risk of nucleic acid degradation, improves the extraction rate, is suitable for subsequent reactions, and is easy to observe.
Smart Images
Figure CN223906837U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to experimental auxiliary instrument technical field, concretely is a kind of grinding rod for extracting trace tissue nucleic acid. BACKGROUND
[0002] With the continuous development of life science field, researchers pay more and more attention to the differences in genotype and gene expression between single cells, trace tissues and single micro-model organisms. Researchers need to obtain trace DNA or RNA for subsequent reactions. The most typical is the application of Smart-seq and STRT-seq in trace tissue and single micro-model organism DNA or RNA library construction. For single cells, researchers put target cells into PCR tubes containing lysis solution, and then perform lysis and reverse transcription procedures. Unlike cells, to enable trace tissues to be fully lysed and release as much RNA as possible, a grinding step needs to be added before the lysis procedure. For grinding small amounts of tissue, commercially available small grinding rods can be selected.
[0003] Fisher Scientific's Kimble TM Kontes TM Pellet Pestle TM for resuspending proteins and DNA in centrifuge tubes or grinding soft tissues. Made of polypropylene for high temperature and pressure sterilization, free of nuclease and pyrogen. Two specifications are available. The 6cm long grinding rod is suitable for 0.5ml centrifuge tubes. The 14cm long grinding rod is suitable for 1.5ml centrifuge tubes.
[0004] The existing grinding rod mainly has the following problems:
[0005] First, the volume is too large. Trace tissue grinding is usually carried out in a small amount of liquid (2-4 μl). The grinding tools currently used are usually suitable for 0.5ml or 1.5ml centrifuge tubes, which are too large in volume for trace liquids, resulting in insufficient grinding. Moreover, the product after grinding in a 0.5ml or 1.5ml centrifuge tube cannot be directly used for subsequent reactions, and transferring the liquid will further increase the risk of nucleic acid degradation.
[0006] Second, the material has strong adsorption. The material used in the existing grinding tool does not consider the influence of adsorption on the extraction rate of nucleic acid. Strong adsorption leads to greater loss of trace liquid.
[0007] Third, non-transparent material is used. For trace tissue grinding, the grinding degree cannot be judged by the naked eye, and it needs to be observed under a microscope at all times. The use of non-transparent material affects the observation effect and makes it difficult to accurately judge the grinding degree.
[0008] Therefore, the utility model provides a kind of grinding stick for extracting trace tissue nucleic acid to solve the problems existing in prior art.The utility model can be fully ground to trace tissue or single microorganism, and product can be directly used in subsequent reaction, suitable for library construction, genotyping, fluorescent quantitative PCR etc. Utility model content
[0009] The utility model discloses a grinding stick 1 matched with the tip of the grinding tube 2 is arranged, and a low adsorption coating is arranged on the grinding head of the grinding stick 1, so that the grinding efficiency is improved and the loss of sample is reduced.
[0010] The utility model provides a kind of grinding stick for extracting trace tissue nucleic acid, including grinding stick 1 and grinding tube 2, the grinding tube 2 is PCR tube, one end of the grinding stick 1 is grinding head 13, the grinding head 13 with the inside tube wall of the tip of the grinding tube 2 is mutually adapted, so that the grinding head 13 can be closely attached to the inner wall of the tip of the grinding tube 2, the grinding stick 1 further include the grinding rod 12 connected with the grinding head 13 and the connecting portion 11 connected with grinding rod 12, the connecting portion 11 can be connected to external equipment and carry out electric or manual grinding.
[0011] Further, the diameter of the grinding rod 12 is less than the outer diameter of the grinding head 13, to facilitate the observation of the inside of the grinding tube 2.
[0012] Further, the grinding stick 1 is composed of solid transparent polypropylene, to facilitate the user to observe the grinding state with microscope.
[0013] Further, the surface of the grinding head 13 is coated with a low adsorption coating, and the low adsorption coating is polytetrafluoroethylene with light transmission.
[0014] Further, the grinding tube 2 is a 0.1ml or 0.2ml PCR tube, and the whole is composed of transparent polypropylene. The product after grinding can be directly used in subsequent reaction.
[0015] Compared with prior art, the grinding stick for extracting trace tissue nucleic acid has the following technical advantages:
[0016] By using PCR tube and matched grinding stick, on the one hand, the grinding product can be directly used in subsequent reaction, reducing the risk of nucleic acid degradation and improving work efficiency, on the other hand, the nucleic acid extraction rate can be improved; by arranging low adsorption coating on the grinding head 13, the liquid after grinding can be avoided to be taken out, reducing sample loss; by using transparent material, the operator can observe conveniently.
[0017] Other features and advantages of the utility model will be described in detail in the subsequent specific implementation manner part. Attached Figure Description
[0018] Fig. 1 This is a schematic diagram of the overall structure of this utility model;
[0019] Fig. 2 This is a schematic diagram of the structure of the grinding rod of this utility model.
[0020] The attached figures are labeled as follows:
[0021] 1-Grinding rod; 2-Grinding tube; 11-Connecting part; 12-Grinding rod; 13-Grinding head. Detailed Implementation
[0022] To further illustrate the technical means and effects adopted by this utility model in order to achieve the intended utility model purpose, the following detailed description of the specific implementation methods, structure, features and effects of this utility model is provided in conjunction with the accompanying drawings and preferred embodiments.
[0023] Example: A grinding rod for extracting trace amounts of tissue nucleic acid
[0024] like Figs. 1-2 As shown
[0025] A grinding rod for extracting trace amounts of tissue nucleic acid includes a grinding rod 1 and a grinding tube 2, wherein the grinding tube 2 is a PCR tube, and one end of the grinding rod 1 is a grinding head 13. The grinding head 13 is adapted to the inner wall of the tip of the grinding tube 2, so that the grinding head 13 can fit tightly against the inner wall of the tip of the grinding tube 2. The grinding rod 1 also includes a grinding rod 12 connected to the grinding head 13 and a connecting part 11 connected to the grinding rod 12. The connecting part 11 can be connected to an external device for electric or manual grinding.
[0026] In this embodiment, the diameter of the grinding rod 12 is smaller than the outer diameter of the grinding head 13, which facilitates observation of the inside of the grinding tube 2.
[0027] In this embodiment, the grinding rod 1 is made of solid transparent polypropylene, which makes it convenient for users to observe the grinding state with a microscope.
[0028] In this embodiment, the surface of the grinding head 13 is coated with a low-adsorption coating, which is a transparent polytetrafluoroethylene.
[0029] In this embodiment, the grinding tube 2 is a 0.1ml PCR tube, entirely made of transparent polypropylene. This allows the ground product to be directly used in subsequent reactions.
[0030] The liquid for the subsequent reaction is added into the grinding tube 2, and the target tissue is transferred into the grinding tube 2 under the microscope. The target is ground by the grinding head 13 of the grinding rod 1 closely contacting with the grinding tube. The operator can choose to manually rotate the grinding rod or use an external device to grind. The operator needs to pay attention to the grinding state under the microscope throughout the whole process. The whole grinding process takes 30s-1min. After the grinding is completed, the product can be directly used for the subsequent reaction.
[0031] The above is only a preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with a preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make slight changes or modifications to the above disclosed technical content to obtain equivalent embodiments with equivalent changes, without departing from the technical solution of the present application. Any modification, equivalent change and modification of the above embodiments, which does not depart from the technical solution of the present application, is still within the scope of the technical solution of the present application.
Claims
1. A grinding rod for extracting trace amounts of tissue nucleic acid, characterized in that: The device includes a grinding rod (1) and a grinding tube (2), wherein the grinding tube (2) is a PCR tube, and one end of the grinding rod (1) is a grinding head (13). The grinding head (13) is adapted to the inner wall of the tip of the grinding tube (2), so that the grinding head (13) can fit tightly against the inner wall of the tip of the grinding tube (2), and a low adsorption coating is provided on the surface. The grinding rod (1) also includes a grinding rod (12) connected to the grinding head (13) and a connecting part (11) connected to the grinding rod (12). The connecting part (11) can be connected to external equipment for electric / manual grinding.
2. The grinding rod for extracting trace amounts of tissue nucleic acid according to claim 1, characterized in that: The low-adsorption coating is made of transparent polytetrafluoroethylene.
3. A grinding rod for extracting trace amounts of tissue nucleic acid according to claim 1, characterized in that: The grinding tube (2) is a 0.1 ml or 0.2 ml PCR tube.
4. A grinding rod for extracting trace amounts of tissue nucleic acid according to claim 1, characterized in that: The diameter of the grinding rod (12) is smaller than the outer diameter of the grinding head (13).
5. A grinding rod for extracting trace amounts of tissue nucleic acids to construct a DNA library according to claim 1, characterized in that: The grinding rod (1) is made of solid transparent polypropylene.