Device for establishing chronic unpredictable stress mouse model

Abstract

The utility model relates to the field of biomedical research, in particular to a device for establishing a chronic unpredictable stress mouse model, which comprises a test bed, an experiment box, a barrier, a glass observation door, a feeding component and the like, the upper side of the test bed is connected with the experiment box, and the lower part of the experiment box is connected with the barrier in a sliding and detachable manner; the front lower part of the experiment box is rotatably connected with a glass observation door, and the left part and the right part of the experiment box are provided with feeding assemblies for feeding the mice with grains and water. The device sprays water through the water sprayer, monitors through the monitoring head, stimulates through lighting of the irradiation lamp, makes contact with a mouse through movement of the brush to carry out patting stimulation, and meanwhile cleans and collects excrement, so that the excrement can be cleaned and collected while stimulation testing is carried out on the mouse through various stimulation sources; various tests of water spraying, strong light and light beating are combined, interference is reduced, the accuracy and reliability of the test are improved, and the device is convenient to use.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biomedical research, especially relate to a device for establishing chronic unpredictable stress mouse model. BACKGROUND

[0002] In the field of biomedical research, especially in the field of neuroscience, psychology and pharmacology, establishing a chronic unpredictable stress mouse model is crucial for understanding the stress response mechanism, exploring the pathophysiological process of stress-related diseases and evaluating the efficacy of potential therapeutic drugs. The CUS model can realistically reflect the physiological and psychological changes of humans when facing long-term and variable stress by simulating unpredictable stressors in real life.

[0003] The use of existing mouse model experimental equipment usually involves placing mice in a test box and then stimulating them through light, electric shock, and temperature control. However, current experimental equipment can only stimulate mice for testing and cannot clean and collect feces during detection. This not only affects the hygiene of the experimental environment, causing odors in the test box, but also may interfere with subsequent behavioral observation and data analysis, reducing the accuracy and reliability of the experiment, making it inconvenient to use.

[0004] Therefore, a device for establishing a chronic unpredictable stress mouse model is needed that can stimulate mice for testing through multiple stimulus sources while cleaning and collecting feces, combining water drenching, strong light, and patting for testing, reducing interference, improving the accuracy and reliability of testing, and being easy to use. UTILITY MODEL CONTENT

[0005] To overcome the shortcomings of current experimental equipment that cannot clean and collect feces during detection, affecting the hygiene of the experimental environment, causing odors in the test box, and possibly interfering with subsequent behavioral observation and data analysis, reducing the accuracy and reliability of the experiment, and being inconvenient to use, the utility model provides a device for establishing a chronic unpredictable stress mouse model that can stimulate mice for testing through multiple stimulus sources while cleaning and collecting feces, combining water drenching, strong light, and patting for testing, reducing interference, improving the accuracy and reliability of testing, and being easy to use.

[0006] The technical scheme of the utility model is: a device for establishing a chronic unpredictable stress mouse model, including test table, experimental box, fence, glass observation door, feeding assembly and test assembly, the test table upper side is connected with experimental box, the experimental box lower part is slidably connected with fence, the experimental box front lower part is rotatably connected with glass observation door, the experimental box left part and right part are equipped with feeding assembly for feeding mice with food and water, the experimental box is equipped with test assembly for stress testing of mice.

[0007] As a preferred technical scheme of the utility model, the limit rod is arranged on the upper front side of the experiment box in a rotating mode.

[0008] As a preferred technical scheme of the utility model, the feeding assembly comprises a feeder, a food box, a water injector and a water box, the feeder is connected to the right part of the experiment box, the food box is connected to the lower right part of the experiment box, the water injector is connected to the left part of the experiment box, and the water box is connected to the inner side of the lower left part of the experiment box.

[0009] As a preferred technical scheme of the utility model, the test assembly comprises a monitoring head, an irradiation lamp, a fan, a water sprayer, a brush and an electric push rod, the monitoring head is connected to the inner sides of the left and right parts of the experiment box, the irradiation lamp is connected to the rear part of the experiment box, the fan is connected to the rear sides of the left and right parts of the experiment box, the water sprayer is connected to the upper part of the experiment box, the electric push rod is connected to the front side of the rear part of the test bench and comprises two parts, the telescopic ends of the electric push rod pass through the experiment box, the brush is connected between the telescopic ends of the electric push rod, and the brush is in contact with the grating.

[0010] As a preferred technical scheme of the utility model, the tray is further arranged, and the tray is slidably and detachably connected to the lower part of the experiment box.

[0011] As a preferred technical scheme of the utility model, the grating and the tray are both provided with a handle on the right side.

[0012] Beneficial effects: 1. The utility model discloses a water sprayer, a monitoring head, an irradiation lamp, a brush and a water sprayer, which can stimulate the mouse through multiple stimulation sources, clean and collect the excrement, realize the combination of water spraying, strong light and patting, reduce interference, improve the accuracy and reliability of the test, and are convenient to use.

[0013] 2. The utility model discloses a handle for moving the grating and the tray, then pouring out the excrement in the tray for cleaning, and then cleaning the grating, so that the grating and the tray can be detached, the excrement is easy to handle, and the grating and the tray are easy to clean.

[0014] 3. The utility model discloses a feeder for feeding, so that the food in the feeder falls into the food box for the mouse to eat, and then a water injector is used for feeding water, so that the water in the water injector drops into the water box for the mouse to drink, so that the mouse can be fed and watered during the test, the additional stress reaction of the mouse due to hunger or thirst is prevented, the experimental results are prevented from being confused, and the physiological state of the mouse is maintained. DRAWINGS

[0015] Figure 1 It is a three-dimensional structure schematic view of the utility model.

[0016] Figure 2 This is a schematic diagram of the internal structure of the experimental box of this utility model.

[0017] Figure 3 This is a plan view of the internal structure of the experimental box of this utility model.

[0018] Figure 4 This is a three-dimensional structural diagram of the fan and electric push rod components of this utility model.

[0019] Figure 5 This is a three-dimensional cross-sectional view of the components of this utility model, such as the grating, tray, and brush.

[0020] The following are the markings in the diagram: 1-Experimental bench, 2-Experimental box, 3-Grid, 4-Tray, 5-Glass observation door, 6-Feeder, 7-Food box, 8-Water injector, 9-Water box, 10-Monitoring head, 11-Illumination lamp, 12-Fan, 13-Water sprayer, 14-Brush, 15-Electric push rod. Detailed Implementation

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, but this does not limit the scope of protection and application of the present invention.

[0022] A device for establishing a mouse model of chronic unpredictable stress, such as Figures 1-5 As shown, the apparatus includes a test bench 1, a test chamber 2, a partition 3, a tray 4, a glass observation door 5, a feeder 6, a food container 7, a water dispenser 8, a water box 9, a monitoring head 10, an illumination lamp 11, a fan 12, a water sprinkler 13, a brush 14, and an electric push rod 15. The test chamber 2 is connected to the upper side of the test bench 1. A rotatable limit rod is installed on the upper front of the test chamber 2. The partition 3 is detachably connected to the lower part of the test chamber 2, and the tray 4 is also detachably connected to the lower part of the test chamber 2. The tray 4 is located below the partition 3. Handles are provided on the right side of both the partition 3 and the tray 4 for easy removal and collection of feces. The lower front of the test chamber 2 has a rotatable limit rod. A glass observation door 5 is connected to the experimental chamber 2. A feeder 6 is connected to the right side of the experimental chamber 2. A food box 7 is connected to the lower right side of the experimental chamber 2. A water injector 8 is connected to the left side of the experimental chamber 2. A water box 9 is connected to the inner side of the lower left side of the experimental chamber 2. Monitoring heads 10 are connected to the inner sides of both the left and right sides of the experimental chamber 2. An illumination lamp 11 is connected to the rear of the experimental chamber 2. Fans 12 are connected to the rear sides of both the left and right sides of the experimental chamber 2. Three water sprinklers 13 are connected to the top of the experimental chamber 2. Two electric push rods 15 are connected to the front of the rear of the experimental platform 1. The telescopic ends of the electric push rods 15 pass through the experimental chamber 2. A brush 14 is connected between the telescopic ends of the electric push rods 15. The brush 14 is in contact with the grid 3.

[0023] When stress testing is required on mice, this device can be used. The test table 1 is brought into contact with the tabletop or work surface. Then, the handle is used to lift the grid 3 and tray 4 and bring them into contact with the test chamber 2. The grid 3 and tray 4 are then moved and installed. The mouse is placed on the grid 3, and the glass observation door 5 is closed. The limiting rod is then rotated to limit the glass observation door 5. Feeding is then performed through the feeder 6, causing the food in the feeder 6 to fall into the food box 7 for the mouse to eat. Water is then provided through the water dispenser 8, causing the water in the water dispenser 8 to... Water is dripped into water box 9 for the mice to drink, allowing for feeding and hydration during testing. This prevents the mice from experiencing additional stress due to hunger or thirst, which could confuse the experimental results and helps maintain their physiological state. Mouse feces remain on the grid 3, while urine flows along the grid 3 into tray 4 for collection. Fan 12 is then activated to ventilate the experimental chamber 2. An external water source is connected to water sprayer 13, which sprays water onto the mice, thus stimulating them. The behavior of the mouse is monitored by the monitoring head 10. Then, the illumination lamp 11 is turned on to stimulate the mouse. The electric push rod 15 is then activated, which moves the brush 14 to contact the mouse and gently tap it. At the same time, the feces on the grid 3 are swept away and fall into the tray 4 for collection. This allows for stimulation testing of the mouse with multiple stimuli while simultaneously cleaning and collecting feces, combining water spray, strong light, and tapping tests to reduce the need for other methods. To reduce interference and improve the accuracy and reliability of testing, this device is easy to use. After testing, turn off the illumination lamp 11, fan 12, and water sprayer 13. Then, reverse the operation of the electric push rod 15 to move the brush 14 in the opposite direction and reset it. Then, pull the handle to move and remove the grid 3 and tray 4. Pour out the feces in the tray 4 for cleaning, and then clean the grid 3. This allows the grid 3 and tray 4 to be disassembled for easy handling of feces and cleaning of the grid 3 and tray 4. Finally, the grid 3 and tray 4 can be reinstalled.

[0024] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. An apparatus for establishing a chronic unpredictable stress mouse model, characterized in that: The utility model relates to a mouse stress test device, including test bench (1), experiment box (2), grille (3), glass observation door (5), feeding assembly and test assembly, test bench (1) upper side is connected with experiment box (2), and experiment box (2) lower part is slidably detachably connected with grille (3), and experiment box (2) lower part is rotatably connected with glass observation door (5), and the left part and right part of experiment box (2) are equipped with feeding assembly for feeding food and water to mouse, and the upper part of experiment box (2) is equipped with test assembly for carrying out stress test to mouse, test assembly includes monitoring head (10), irradiation lamp (11), fan (12), shower (13), brush (14) and electric push rod (15), and the inboard of the left and right two parts of experiment box (2) is connected with monitoring head (10), and the rear part of experiment box (2) is connected with irradiation lamp (11), and the rear side of the left and right two parts of experiment box (2) is connected with fan (12), and the upper part of experiment box (2) is connected with a plurality of shower (13), and the front side of the rear part of test bench (1) is connected with left and right two electric push rods (15), and the telescopic end of electric push rod (15) all passes through experiment box (2), and the telescopic end of electric push rod (15) all is connected with brush (14), and brush (14) is in contact with grille (3).

2. The apparatus for establishing a chronic unpredictable stress mouse model according to claim 1, wherein: The upper front side of experiment box (2) is rotatably provided with a limiting rod.

3. The apparatus for establishing a chronic unpredictable stress mouse model according to claim 1, wherein: Feeding assembly includes feeder (6), food box (7), water injector (8) and water box (9), and the right part of experiment box (2) is connected with feeder (6), and the lower part of experiment box (2) is connected with food box (7), and the left part of experiment box (2) is connected with water injector (8), and the inboard of the lower part of experiment box (2) is connected with water box (9).

4. The apparatus for establishing a chronic unpredictable stress mouse model according to claim 1, wherein: It also includes a tray (4), and the lower part of experiment box (2) is slidably detachably connected with tray (4), and tray (4) is located the lower side of grille (3).

5. The apparatus for establishing a chronic unpredictable stress mouse model according to claim 4, wherein: The right side of grille (3) and tray (4) is provided with a handle.

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