Reagent dropping device for fluorescence immunoassay

Abstract

The utility model provides a reagent dropping device for fluorescence immunoassay, which belongs to the technical field of dropping devices and comprises a base and a fastening screw, a guide rod is fixedly arranged on the base, and a mounting plate is slidably arranged on the guide rod in a limiting manner; a movable rod is arranged on the mounting plate in a limiting and sliding manner, a reagent tube is mounted on the mounting plate, and the mounting plate and the movable rod are fixed through fastening screws; a connecting plate is fixedly arranged at the top of the movable rod and provided with a through hole. A piston rod is fixedly arranged at the top of the piston, and the top of the piston rod penetrates through the through hole; a movable sleeve is slidably arranged on the periphery of the piston rod in the radial direction of the piston rod, and the movable sleeve is in transmission fit with the rotating rod; the outer cambered surface of the movable sleeve is provided with an external thread, and an internal thread in threaded fit with the external thread is arranged in the through hole. Threaded fit between the movable sleeve and the through hole can be relieved only by rotating the rotating rod, the piston can be conveniently and rapidly pulled up, and the using flexibility is improved.

Description

Technical Field

[0001] This utility model belongs to the field of dripping device technology, specifically relating to a reagent dripping device for fluorescence immunoassay. Background Technology

[0002] Immunofluorescence is one of the earliest developed labeling immunoassay techniques, built upon immunology, biochemistry, and microscopy. In immunofluorescence detection, reagents such as proteins, hormones, drugs, and microorganisms are added dropwise to extract small amounts for immunofluorescence detection. However, existing dropwise devices cannot accurately quantify the added reagents; therefore, a reagent dropwise device for immunofluorescence detection is urgently needed.

[0003] A patent with publication number CN213000096U discloses a reagent dispensing device for fluorescence immunoassay. The device includes a movable mounting bracket, a locking nut, a positioning thread, a guide rod, a scale bar, a stopper rod, a connecting seat, and a dropper. In use, the guide rod is first fixed in place by the locking nut and positioning nut, thus determining the position of the scale bar. Then, the stopper rod is rotated, driving it to move spirally at a uniform speed on the connecting seat to ensure uniform dispensing of the reagent in the dropper. This continues until the end of the stopper rod contacts and is pressed against the top of the connecting seat, thus achieving precise dispensing.

[0004] Due to the inherent characteristics of threaded connections, the above solution cannot achieve rapid rise of the stopper after the reagent is added, thus hindering rapid reagent extraction. This delays the reagent extraction time of the dropper and reduces the extraction rate, exhibiting certain limitations. Utility Model Content

[0005] To address the problems existing in the background art, this utility model provides a reagent dispensing device for fluorescence immunoassay.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A reagent dispensing device for fluorescence immunoassay includes a base and fastening screws. A guide rod is fixedly mounted on the base, and a mounting plate is slidably mounted on the guide rod. A movable rod is slidably mounted on the mounting plate, and a reagent tube is also mounted thereon. Both the mounting plate and the movable rod are fixed by fastening screws. A connecting plate is fixedly mounted on the top of the movable rod, and a through hole is formed on the connecting plate. A piston is slidably mounted inside the reagent tube, and a piston rod is fixedly mounted on the top of the piston, with the top of the piston rod passing through the through hole. A rotating rod is rotatably mounted inside the piston rod, and a movable sleeve is slidably mounted on the outer periphery of the piston rod along its radial direction, with a transmission engagement between the movable sleeve and the rotating rod. The outer arc surface of the movable sleeve has an external thread, and the through hole has an internal thread that mates with the external thread. A gripping plate is fixedly mounted on the top of the piston rod, and the top of the rotating rod rotatably passes through the gripping plate. A limiting element acting on the rotating rod is provided on the gripping plate.

[0008] Furthermore, a plurality of sliding rods are slidably disposed on the outer surface of the piston rod, and the plurality of sliding rods slide along the radial direction of the piston rod; one end of the sliding rod located outside the piston rod is fixedly connected to the inner wall of the movable sleeve, and an arc-shaped block is fixedly disposed on the other end of the sliding rod located inside the piston rod; a second spring is sleeved on the outer surface of the sliding rod located outside the piston rod, one end of the second spring is fixedly connected to the inner wall of the movable sleeve, and the other end is fixedly connected to the outer surface of the piston rod.

[0009] Furthermore, a wedge-shaped rod is fixedly sleeved on the outer surface of the rotating rod. The cross-section of the wedge-shaped rod is elliptical, and the wedge-shaped rod is engaged with the arc-shaped block in a transmission manner.

[0010] Furthermore, the limiting component includes a limiting block, a movable block is keyed to the outer surface of the rotating rod, the movable block is located above the grip plate, and a first spring is sleeved on the outer surface of the rotating rod; a limiting block is fixedly installed at the bottom of the movable block, and two limiting grooves that cooperate with the limiting blocks are opened at the top of the grip plate, forming a 90° angle between the two limiting grooves.

[0011] This application has the following beneficial effects:

[0012] 1. This design allows the rotating rod to achieve a threaded engagement between the movable sleeve and the through hole. This, in turn, allows the piston to move helically via the rotating gripper, driving the piston to move at a uniform speed within the reagent tube, ensuring even dispensing of the reagent. Simultaneously, when reagent extraction is required, simply rotating the rotating rod disengages the threaded engagement between the movable sleeve and the through hole, facilitating rapid piston retraction and quick extraction of the reagent from the tube. This enhances the flexibility and practicality of the device.

[0013] 2. This solution removes the threaded fit between the movable sleeve and the through hole, facilitating the rapid downward pushing of the piston and enabling rapid reagent addition, further improving the flexibility and practicality of the device during use. Attached Figure Description

[0014] The above and other objects, features, and advantages of the present invention will become readily understood by reading the following detailed description of exemplary embodiments with reference to the accompanying drawings. In the drawings, several embodiments of the present invention are shown by way of example and not limitation, and like or corresponding reference numerals denote like or corresponding parts, wherein:

[0015] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0016] Figure 2 This is a partial cross-sectional view of the present invention;

[0017] Figure 3 This is a utility model Figure 2 A magnified view of a portion of point A in the middle;

[0018] Figure 4 This is a utility model Figure 2 A magnified view of a portion of point B in the middle;

[0019] Figure 5 This is a schematic diagram of the limiting groove structure of this utility model;

[0020] Figure 6 This is a schematic diagram of the connection structure between the movable sleeve and the piston rod of this utility model.

[0021] Explanation of reference numerals in the attached figures:

[0022] 1. Base; 2. Guide rod; 3. Mounting plate; 4. Fastening screw; 5. Reagent tube; 6. Scale line; 7. Movable rod; 8. Connecting plate; 9. Rotating rod; 10. Movable block; 11. Grip plate; 12. Piston rod; 13. Wedge rod; 14. Movable sleeve; 15. Limiting block; 16. First spring; 17. Piston; 18. Through hole; 19. Slide rod; 20. Second spring; 21. Arc-shaped block; 22. Limiting groove. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Those skilled in the art should understand that the embodiments described below are only some, not all, of the embodiments disclosed. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0024] like Figures 1-6As shown, the technical solution adopted by this utility model is as follows: A reagent dispensing device for fluorescence immunoassay includes a base 1 and a fastening screw 4. A guide rod 2 is fixedly installed on the top of the base 1. An installation plate 3 is slidably installed on the outer surface of the guide rod 2 in the vertical direction. A reagent tube 5 is installed on the installation plate 3, and a movable rod 7 is slidably installed on it. The installation plate 3 and the movable rod 7 are both fixed by the fastening screw 4.

[0025] A scale line 6 is fixedly installed on the outer surface of the movable rod 7, and a connecting plate 8 is fixedly installed on the top of the movable rod 7. A through hole 18 is opened on the connecting plate 8. A piston 17 is sealed and slidably installed inside the reagent tube 5. A piston rod 12 is fixedly installed on the top of the piston 17. The top of the piston rod 12 passes through the through hole 18 and is fixedly installed with a grip plate 11.

[0026] By adjusting the height of the movable rod 7 and limiting it with the fastening screw 4, the dosage of reagent is determined according to the scale line 6. Press the grip plate 11 until the bottom of the grip plate 11 abuts against the top of the connecting plate 8. The connecting plate 8 limits the distance the grip plate 11 descends, thereby achieving precise reagent dispensing.

[0027] A rotating rod 9 is rotatably mounted inside the piston rod 12. A movable sleeve 14 is slidably mounted on the outer periphery of the piston rod 12 along its radial direction. The movable sleeve 14 is in a transmission engagement with the rotating rod 9. In this embodiment, there are two movable sleeves 14, located on both sides of the piston rod 12. The outer surface of the movable sleeve 14 is fixedly provided with an external thread, and the inner wall of the through hole 18 is fixedly provided with an internal thread that engages with the external thread.

[0028] like Figure 4 and Figure 6 As shown, a plurality of slide rods 19 are slidably disposed on the outer surface of the piston rod 12. The slide rods 19 slide radially along the piston rod 12. One end of the slide rod 19 located outside the piston rod 12 is fixedly connected to the inner wall of the movable sleeve 14, and an arc-shaped block 21 is fixedly disposed on the other end of the slide rod 19 located inside the piston rod 12. A second spring 20 is sleeved on the outer surface of the slide rod 19 located outside the piston rod 12. One end of the second spring 20 is fixedly connected to the inner wall of the movable sleeve 14, and the other end is fixedly connected to the outer surface of the piston rod 12.

[0029] A wedge-shaped rod 13 is fixedly sleeved on the outer surface of the rotating rod 9. The cross-section of the wedge-shaped rod 13 is elliptical, and the wedge-shaped rod 13 is in transmission cooperation with the arc-shaped block 21.

[0030] By rotating the rotating rod 9, the wedge rod 13 is driven to rotate, causing the arc-shaped block 21 to slide away from the central axis of the rotating rod 9, thereby pushing the movable sleeve 14 to slide synchronously, so that the external thread of the movable sleeve 14 engages with the internal thread of the through hole 18. Then, the grip plate 11 is rotated to perform helical displacement, so that the reagent is slowly and uniformly added.

[0031] like Figure 3and Figure 5 As shown, the top of the rotating rod 9 rotates through the grip plate 11 and is integrally provided with a disc. The grip plate 11 is provided with a limiting member that acts on the rotating rod 9.

[0032] The limiting component includes a limiting block 15. A movable block 10 is keyed to the outer surface of the rotating rod 9. The movable block 10 is located above the grip plate 11. A first spring 16 is provided between the movable block 10 and the disc. The first spring 16 is sleeved on the outer surface of the rotating rod 9. One end of the first spring 16 is fixedly connected to the disc, and the other end is fixedly connected to the movable block 10. The limiting block 15 is fixedly installed at the bottom of the movable block 10. Two limiting grooves 22 are opened at the top of the grip plate 11 to limit and cooperate with the limiting block 15. The two limiting grooves 22 form a 90° angle between them.

[0033] By pulling the movable block 10, the limiting block 15 can be disengaged from the limiting groove 22, thus releasing the limiting of the rotating rod 9. Conversely, the limiting of the rotating rod 9 can be restored by using the elasticity of the first spring 16 to press the limiting block 15 against the limiting groove 22.

[0034] Working principle: In the initial state, the external thread of the movable sleeve 14 does not engage with the internal thread of the through hole 18. In use, place the external beaker on the base 1, allowing the mounting plate 3 to slide on the guide rod 2. Once the mounting plate 3 has slid to the appropriate position, rotate the fastening screw 4 to limit its movement, thereby fixing the position of the reagent tube 5.

[0035] Then adjust the position of the movable rod 7 so that it slides up and down. When the movable rod 7 slides to the appropriate position, rotate the fastening screw 4 to limit it, thereby determining the dosage of reagent to be added.

[0036] Use case 1: When a certain amount of reagent needs to be added quickly, press down on the grip plate 11 until the grip plate 11 touches the upper surface of the connecting plate 8. At this time, a certain amount of reagent in the reagent tube 5 is quickly squeezed into the beaker, realizing the rapid and accurate addition of the reagent.

[0037] Use case 2: When reagents need to be added slowly and at a constant rate, pull the movable block 10 upward and squeeze the first spring 16. The movable block 10 and the rotating rod 9 slide relative to each other, so that the limiting block 15 disengages from the limiting groove 22 in the initial state.

[0038] Then, the movable block 10 is rotated, and the movable block 10 drives the rotating rod 9 to rotate synchronously through the key connection. The rotating rod 9 drives the wedge rod 13 to rotate synchronously, so that the arc block 21 slides away from the central axis of the rotating rod 9, thereby pushing the movable sleeve 14 to slide synchronously, and the second spring 20 is gradually stretched.

[0039] When the movable block 10 rotates 90°, it is released and slides downward under the elastic action of the first spring 16, causing the limiting block 15 to engage in another limiting groove 22, thus restoring the limiting of the rotating rod 9. At this time, the external thread of the movable sleeve 14 is exactly engaged with the internal thread of the through hole 18.

[0040] Next, rotate the grip plate 11 to make a spiral displacement, so that the reagent is slowly and evenly added until the grip plate 11 contacts the upper surface of the connecting plate 8, thereby achieving uniform and precise addition of the reagent.

[0041] Use case 3: When reagent tube 5 needs to extract reagents at a relatively fast speed, pull the movable block 10 upward and rotate the movable block 10 in the opposite direction so that the limiting block 15 is locked back into the limiting groove 22 in the initial state. The movable sleeve 14 is reset under the elastic action of the second spring 20, thereby releasing the threaded engagement between the movable sleeve 14 and the through hole 18.

[0042] Then, pull up the grip 11 to quickly extract the reagent.

[0043] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A reagent dispensing device for fluorescence immunoassay, characterized in that: The system includes a base (1) and fastening screws (4). A guide rod (2) is fixedly mounted on the base (1), and a mounting plate (3) is slidably mounted on the guide rod (2). A movable rod (7) is slidably mounted on the mounting plate (3), and a reagent tube (5) is also mounted on it. Both the mounting plate (3) and the movable rod (7) are fixed by fastening screws (4). A connecting plate (8) is fixedly mounted on the top of the movable rod (7), and a through hole (18) is opened on the connecting plate (8). A piston (17) is slidably mounted inside the reagent tube (5), and a piston rod (12) is fixedly mounted on the top of the piston (17). The top of the rod (12) is through a through hole (18); a rotating rod (9) is rotatably arranged inside the piston rod (12), and a movable sleeve (14) is slidably arranged around the piston rod (12) along the radial direction of the piston rod (12), and the movable sleeve (14) and the rotating rod (9) are in a transmission fit; the outer arc surface of the movable sleeve (14) is provided with an external thread, and the through hole (18) is provided with an internal thread that fits with the external thread; a gripping plate (11) is fixedly arranged on the top of the piston rod (12), and the top of the rotating rod (9) rotatably passes through the gripping plate (11), and a limiting member acting on the rotating rod (9) is provided on the gripping plate (11).

2. The reagent dispensing device for fluorescence immunoassay according to claim 1, characterized in that: A plurality of slide rods (19) are slidably connected through the outer surface of the piston rod (12), and the slide rods (19) slide radially along the piston rod (12); one end of the slide rod (19) located outside the piston rod (12) is fixedly connected to the inner wall of the movable sleeve (14), and one end of the slide rod (19) located inside the piston rod (12) is fixedly provided with an arc-shaped block (21); a second spring (20) is sleeved on the outer surface of the slide rod (19) located outside the piston rod (12), one end of the second spring (20) is fixedly connected to the inner wall of the movable sleeve (14), and the other end is fixedly connected to the outer surface of the piston rod (12).

3. The reagent dispensing device for fluorescence immunoassay according to claim 2, characterized in that: The outer surface of the rotating rod (9) is fixedly fitted with a wedge rod (13), the cross section of the wedge rod (13) is elliptical, and the wedge rod (13) is in transmission cooperation with the arc block (21).

4. The reagent dispensing device for fluorescence immunoassay according to claim 1, characterized in that: The limiting component includes a limiting block (15), and a movable block (10) is keyed to the outer surface of the rotating rod (9). The movable block (10) is located above the grip plate (11), and a first spring (16) is sleeved on the outer surface of the rotating rod (9). The limiting block (15) is fixedly installed at the bottom of the movable block (10), and two limiting grooves (22) that cooperate with the limiting block (15) are opened at the top of the grip plate (11). The two limiting grooves (22) form a 90° angle.

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