Industrial cannabis sativa pollen tube channel genetic transformation vacuum device

Abstract

The utility model is suitable for the technical field of plant vacuum infection, and provides an industrial cannabis sativa L. pollen tube channel genetic transformation vacuum device which comprises a vacuum tank used for providing an industrial cannabis sativa L. infection environment. The acrylic cover plate is detachably mounted at the top of the vacuum tank through nuts and bolts and is used for sealing the vacuum tank; the sealing rubber ring is arranged between the vacuum tank and the acrylic cover plate and is used for increasing sealing; the pressure gauge is mounted at the top of the acrylic cover plate and is used for monitoring the air pressure in the vacuum tank; and the air taps are fixed at the top of the acrylic cover plate, are respectively positioned on two sides of the pressure gauge and are used for exhausting air and feeding air. According to the industrial cannabis sativa pollen tube channel genetic transformation vacuum device provided by the scheme, the whole industrial cannabis sativa plant is inverted for dip dyeing, and the dip dyeing effect is effectively improved.

Description

Technical Field

[0001] This invention belongs to the field of plant vacuum infiltration technology, and particularly relates to a vacuum device for genetic transformation of pollen tube channels in industrial hemp. Background Technology

[0002] Utilizing genetic transformation systems for transgenic or gene editing is of great significance for crop improvement. Compared to model plants, the genetic transformation system for industrial hemp is still imperfect, and the regeneration and transformation efficiency of genetic transformation systems through tissue culture is relatively low. Therefore, research on transgenic and gene editing is relatively lagging behind. Establishing a mature genetic transformation system to support scientific research and industrial development in industrial hemp is an important research direction at present.

[0003] The pollen tube pathway method is a germplasm-mediated method for introducing exogenous genes without relying on a tissue culture system. It directly yields transformed seeds. The pollen tube pathway method involves introducing exogenous DNA through the pollen tube pathways formed after plant pollination, allowing it to penetrate the pollen tube, then through the nucellus into the embryo sac. This transforms eggs, zygotes, or early embryonic cells that lack normal cell walls. Cells in the protoplast stage are more conducive to DNA integration, thus achieving genetic transformation. Agrobacterium-mediated transformation uses Agrobacterium as a medium to introduce T-DNA into the plant genome through infection of meristematic receptors such as explants, callus tissue, and suspension cells. This T-DNA is then specifically expressed in various tissues and organs. Vacuum permeation utilizes atmospheric negative pressure to remove air from the intercellular spaces of plant cells. During the vacuum release phase, Agrobacterium enters the intercellular spaces and infects plant cells, thus increasing the efficiency of Agrobacterium infection. Therefore, conducting Agrobacterium stigma infection in a vacuum environment can significantly improve the infection efficiency of the pollen tube pathway method.

[0004] Because industrial hemp plants are quite tall during the flowering period and have large flower spikes, there is currently no vacuum device that can infect the entire plant with Agrobacterium. Utility Model Content

[0005] This invention provides a vacuum device for genetic transformation of pollen tube channels in industrial hemp, aiming to solve the problem mentioned in the background art that, due to the certain height of mature plants, existing equipment is difficult to infect the entire plant with Agrobacterium.

[0006] To solve the above problems, this utility model is implemented as follows: an industrial hemp pollen tube channel genetic transformation vacuum device, comprising: a vacuum tank for providing an environment for industrial hemp infection; an acrylic cover plate detachably mounted on the top of the vacuum tank via nuts and bolts for sealing the vacuum tank; a sealing rubber ring disposed between the vacuum tank and the acrylic cover plate for increasing the seal; a pressure gauge mounted on the top of the acrylic cover plate for monitoring the air pressure inside the vacuum tank; air nozzles fixed on the top of the acrylic cover plate and located on both sides of the pressure gauge for venting and inlet air; a vacuum pump disposed on one side of the vacuum tank for extracting air from the vacuum tank, the inlet end of the vacuum pump being connected to the air nozzles; and a support assembly disposed inside the vacuum tank for supporting the industrial hemp plant.

[0007] Preferably, the support assembly includes: a base detachably installed inside the vacuum tank; a support rod installed on top of the base; and a support plate and a pot clamp detachably installed on the support rod for stabilizing the industrial hemp plant planting pot, wherein the pot clamp is located above the support plate and in contact with the bottom of the industrial hemp plant planting pot.

[0008] Preferably, the flowerpot clamp is movably sleeved outside the support rod, and a limiting bolt that can contact the support rod is threaded on the flowerpot clamp. A clamp that is locked outside the support rod is installed on the support plate. The limiting bolt and the clamp are used to stabilize the flowerpot clamp and the support plate, respectively.

[0009] Preferably, a clamping plate is installed on the bottom inner wall of the vacuum tank. The clamping plate is L-shaped and is clamped outside the base. The support plate has an opening that allows industrial hemp plants to pass through. A protective structure for reducing the space of the opening is detachably installed on the support plate.

[0010] Preferably, the protective structure includes a connecting frame detachably mounted on the support plate, and an isolation plate fixed to the connecting frame and extending into the opening for sealing the opening, the isolation plate being used to reduce the leakage of planting soil from industrial hemp plants.

[0011] Preferably, both the vacuum tank and the acrylic cover are provided with extension plates, the nut bolt is threadedly installed on one of the extension plates and threadedly connected to the other extension plate, and the top of the vacuum tank is provided with a groove for accommodating the sealing rubber ring.

[0012] Preferably, there are at least two air nozzles, which are used for air intake and air exhaust respectively. The air nozzle used for air intake is covered with a threaded cap for sealing the air nozzle, and the air nozzle used for air exhaust is connected to the air intake end of the vacuum pump. The air nozzle is provided with a one-way valve.

[0013] Preferably, the vacuum canister is equipped with handles on both sides for providing gripping points, and the bottom of the vacuum canister is provided with a support base for supporting the vacuum canister, and the support base is provided with a recess for stabilizing the vacuum canister.

[0014] Compared with related technologies, the industrial hemp pollen tube channel genetic transformation vacuum device provided by this utility model has the following beneficial effects:

[0015] Compared with existing technologies, the industrial hemp pollen tube channel genetic transformation vacuum device provided by this solution optimizes the process of Agrobacterium infection of industrial hemp plants by providing a controllable vacuum environment. The device has excellent sealing performance and can stably maintain the vacuum environment. At the same time, it is easy to disassemble, clean and operate. The flexibility and stability of the support components ensure the fixation of industrial hemp plants during the vacuum infection process. By inverting the plants, they are completely infected with Agrobacterium, which improves the uniformity and efficiency of infection. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main structure of a vacuum device for genetic transformation of industrial hemp pollen tube channels provided by this utility model;

[0017] Figure 2 This is a schematic diagram of the main cross-sectional structure of the vacuum tank in this utility model;

[0018] Figure 3 This is a schematic cross-sectional view of the assembly structure of the support plate, connecting frame and isolation plate in this utility model;

[0019] Figure 4 This is a three-dimensional structural diagram of the assembly of the support plate, connecting frame and isolation plate in this utility model.

[0020] Reference numerals: 1. Vacuum tank; 2. Sealing rubber ring; 3. Acrylic cover plate; 4. Air nozzle; 5. Vacuum pump; 6. Pressure gauge; 7. Nut and bolt; 8. Clamping plate; 9. Base; 10. Support rod; 11. Support plate; 12. Flower pot clamp; 13. Limit bolt; 14. Clamp; 15. Connecting frame; 16. Opening; 17. Isolation plate; 18. Support base. Detailed Implementation

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and foregoing description of the drawings are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or foregoing drawings are used to distinguish different objects, not to describe a particular order; the terms "inner," "outer," "left," and "right" indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0022] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0023] This utility model embodiment provides an industrial hemp pollen tube channel genetic transformation vacuum device, such as... Figure 1-4 As shown, the industrial hemp pollen tube channel genetic transformation vacuum device includes: a vacuum tank 1 for providing an environment for industrial hemp infection; an acrylic cover plate 3 detachably mounted on the top of the vacuum tank 1 via nuts and bolts 7 for sealing the vacuum tank 1; a sealing rubber ring 2 disposed between the vacuum tank 1 and the acrylic cover plate 3 for increasing the seal; a pressure gauge 6 installed on the top of the acrylic cover plate 3 for monitoring the air pressure inside the vacuum tank 1; air nozzles 4 fixed on the top of the acrylic cover plate 3 and located on both sides of the pressure gauge 6 for venting and inlet air; a vacuum pump 5 disposed on one side of the vacuum tank 1 for extracting air from the vacuum tank 1, the inlet end of the vacuum pump 5 being connected to the air nozzle 4; and a support assembly disposed inside the vacuum tank 1 for supporting the industrial hemp plant.

[0024] In this embodiment, the vacuum tank 1 serves as a container providing an environment for industrial hemp infection. Under vacuum conditions, Agrobacterium can more effectively infect plant cells, providing a controllable vacuum environment that is beneficial to the infection process. The acrylic cover 3 is used to seal the vacuum tank 1, while allowing the installation of accessories such as the air nozzle 4 and pressure gauge 6, providing the vacuum tank 1 with airtightness and ensuring the stability of the vacuum environment. It is also easy to disassemble and clean. The sealing rubber ring 2 prevents vacuum leakage and ensures the maintenance of the vacuum environment. The pressure gauge 6 is used to monitor the air pressure inside the vacuum tank 1, providing real-time air pressure data, which is convenient for controlling the vacuum degree and the infection process. The air nozzle 4 is connected to the vacuum pump 5, realizing the extraction of air and the injection of gas inside the vacuum tank 1. It is a key component for controlling the vacuum degree. The vacuum pump 5 provides the power source for the vacuum environment and is a key device for realizing vacuum infection. The support assembly is used to support the industrial hemp plants.

[0025] In a further preferred embodiment of the present invention, the support assembly includes: a base 9 detachably installed inside the vacuum tank 1; a support rod 10 installed on the top of the base 9; and a support plate 11 and a flowerpot clamp 12 detachably installed on the support rod 10 for stabilizing the industrial hemp plant planting pot, wherein the flowerpot clamp 12 is located above the support plate 11 and in contact with the bottom of the industrial hemp plant planting pot.

[0026] In this embodiment, the base 9 serves as the foundation of the support assembly, providing a stable support surface that facilitates the subsequent installation and fixing of the support rod 10, the support plate 11, and the flowerpot clamp 12. The support rod 10 serves as a vertical support structure connecting the base 9, the support plate 11, and the flowerpot clamp 12. The support plate 11 provides additional stability, preventing the planting pot from tilting or shifting due to airflow or gravity in a vacuum environment. The flowerpot clamp 12 further secures the planting pot by closely contacting the bottom of the pot, preventing the plant from shaking or tilting due to airflow disturbance during vacuum contamination, thus ensuring the uniformity and efficiency of contamination.

[0027] In a further preferred embodiment of the present invention, the flowerpot clamp 12 is movably sleeved outside the support rod 10, and a limiting bolt 13 that can contact the support rod 10 is threaded on the flowerpot clamp 12. A clamp 14 that is locked outside the support rod 10 is installed on the support plate 11. The limiting bolt 13 and the clamp 14 are used to stabilize the flowerpot clamp 12 and the support plate 11, respectively.

[0028] In this embodiment, the limiting bolt 13 is used to stabilize the flowerpot clamp 12. By rotating the limiting bolt 13, it can be made to make close contact with the support rod 10, thereby fixing the position of the flowerpot clamp 12 and preventing it from sliding or shaking on the support rod 10. This design ensures that the flowerpot clamp 12 can stably hold the bottom of the industrial hemp plant planting pot, improving the stability of the entire support assembly. The clamp 14, through its structure, tightly wraps around the support rod 10, thereby fixing the position of the support plate 11. This design not only simplifies the installation process of the support plate 11, but also improves its stability, preventing the support plate 11 from shaking or shifting due to airflow disturbance during vacuum impregnation.

[0029] In a further preferred embodiment of the present invention, a clamping plate 8 is installed on the bottom inner wall of the vacuum tank 1. The clamping plate 8 is L-shaped and is clamped outside the base 9. The support plate 11 is provided with an opening 16 that allows industrial hemp plants to pass through. A protective structure for reducing the space of the opening 16 is detachably installed on the support plate 11.

[0030] In this embodiment, the design of the retaining plate 8 provides an additional fixing point for the base 9, enhancing the stability of the base 9 within the vacuum chamber 1. The L-shaped structure allows the retaining plate 8 to fit tightly against the bottom inner wall of the vacuum chamber 1 and the outer side of the base 9, preventing the base 9 from shifting or shaking due to airflow disturbances in a vacuum environment. This design further improves the stability of the entire support assembly, ensuring the smooth progress of the Agrobacterium infection process. The opening 16 allows the stem of the industrial hemp plant to pass through the support plate 11, while the planting pot is placed above the support plate 11. The roots of the plant can still be fully infected by Agrobacterium, while the stem can grow freely through the opening 16. This design ensures the infection effect and avoids the problem of the plant being unable to grow normally due to being completely fixed. The design of the protective structure can adjust the size of the opening 16 as needed to accommodate industrial hemp plant stems of different thicknesses. When the plant is thinner, the space of the opening 16 can be reduced to fix the plant more tightly and prevent it from shaking due to airflow disturbance in a vacuum environment. This design improves the stability of the plant and ensures the uniformity and efficiency of infection.

[0031] In a further preferred embodiment of the present invention, the protective structure includes a connecting frame 15 detachably mounted on the support plate 11, and an isolation plate 17 fixed on the connecting frame 15 and extending into the opening 16 for sealing the opening 16. The isolation plate 17 is used to reduce the leakage of planting soil from industrial hemp plants.

[0032] In this embodiment, the design of the connecting frame 15 allows the isolation plate 17 to be stably installed on the support plate 11 and its position to be adjusted as needed. This design improves the flexibility and adaptability of the protective structure, allowing users to adjust the position and size of the isolation plate 17 according to the growth of the industrial hemp plants and the infection requirements. The design of the isolation plate 17 effectively prevents the planting soil from leaking out of the opening 16 during the vacuum infection process. This not only keeps the vacuum tank 1 clean and hygienic but also reduces the interference of the planting soil on the Agrobacterium infection process.

[0033] In a further preferred embodiment of the present invention, both the vacuum tank 1 and the acrylic cover plate 3 are provided with extension plates, the nut bolt 7 is threadedly installed on any one of the extension plates and threadedly connected to the other extension plate, and the top of the vacuum tank 1 is provided with a groove for accommodating the sealing rubber ring 2.

[0034] In this embodiment, the extension plate design allows the vacuum tank 1 and the acrylic cover plate 3 to be tightly connected by nuts and bolts 7, thereby improving the sealing performance of the entire vacuum device. This design ensures that a stable vacuum environment is maintained inside the vacuum tank 1 during Agrobacterium infection, preventing the entry of external air or contaminants. At the same time, the extension plate also provides sufficient connection area, allowing the nuts and bolts 7 to firmly fix the vacuum tank 1 and the acrylic cover plate 3, preventing leakage or damage caused by loose connection. The groove design provides a stable installation position for the sealing rubber ring 2, ensuring its tight fit between the vacuum tank 1 and the acrylic cover plate 3. As a key component to prevent leakage, the sealing rubber ring 2 effectively improves the sealing performance of the vacuum device.

[0035] In a further preferred embodiment of the present invention, there are at least two air nozzles 4, which are used for air intake and air exhaust respectively. The air nozzle 4 used for air intake is covered with a threaded cap for sealing the air nozzle 4. The air nozzle 4 used for air exhaust is connected to the air intake end of the vacuum pump 5. A one-way valve is provided on the air nozzle 4.

[0036] In this embodiment, the air inlet nozzle 4 is used to introduce air or the required gas into the vacuum container 1, while the air outlet nozzle 4 is used to discharge the gas from the vacuum container 1 to achieve a vacuum or a specific gas environment. By setting no less than two nozzles 4, the air intake and exhaust processes can be controlled separately, improving the flexibility and controllability of the vacuum device. This design allows users to easily adjust the gas composition and pressure in the vacuum container 1 according to experimental needs, thereby optimizing the conditions for Agrobacterium infection. The threaded cap design ensures that the air inlet nozzle 4 can remain closed when not in use, preventing external air or contaminants from entering the vacuum container 1. The one-way valve design ensures that the flow direction of the gas in the vacuum container 1 is controllable, preventing experimental failure or inaccurate results caused by reverse gas flow.

[0037] In a further preferred embodiment of the present invention, handles for providing gripping points are installed on both sides of the vacuum tank 1, and a support base 18 for supporting the vacuum tank 1 is provided at the bottom of the vacuum tank 1, and a recess for stabilizing the vacuum tank 1 is provided in the support base 18.

[0038] In this embodiment, handles are installed on both sides of the vacuum canister 1, providing users with convenient gripping points for easy handling and movement of the vacuum canister 1. This avoids the risk of slippage or damage caused by directly gripping the main body of the vacuum canister 1. This design not only improves the portability of the vacuum canister 1 but also ensures the safety of the user during operation. The design of the support base 18 provides a stable support point for the vacuum canister 1, preventing it from tipping over or sliding due to instability during placement or use. This design not only improves the stability of the vacuum canister 1 but also ensures the safety of the user during operation. The notch can also play a role in positioning and limiting, enabling the vacuum canister 1 to quickly find the correct position and remain fixed when placed.

[0039] In summary, compared with related technologies, this device optimizes the process of Agrobacterium infection of industrial hemp plants by providing a controllable vacuum environment. The device has excellent sealing performance, which can stably maintain the vacuum environment. At the same time, it is easy to disassemble, clean and operate. The flexibility and stability of the support components ensure the fixation of industrial hemp plants during the vacuum infection process. By inverting the plants, they are completely infected with Agrobacterium, which improves the uniformity and efficiency of infection.

[0040] It should be understood, in the several embodiments provided in this application, that the disclosed apparatus may be implemented in other ways.

[0041] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit the scope of protection of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. Although this utility model has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of this utility model according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not fundamentally depart from the concept of this utility model. These technical solutions are also within the scope of protection of this utility model.

Claims

1. An industrial hemp pollen tube channel genetic transformation vacuum device, characterized in that, The utility model relates to an industrial hemp cultivation device, comprising: a vacuum tank for providing an industrial hemp infection environment; a PMMA cover plate detachably mounted on the top of the vacuum tank by a nut bolt for closing the vacuum tank; a sealing rubber ring arranged between the vacuum tank and the PMMA cover plate for increasing the sealing; a pressure gauge mounted on the top of the PMMA cover plate for monitoring the air pressure in the vacuum tank; air nozzles fixed on the top of the PMMA cover plate and respectively located on both sides of the pressure gauge for air intake and air exhaust; a vacuum pump arranged on one side of the vacuum tank for extracting air in the vacuum tank, the air intake end of the vacuum pump being connected with the air nozzle; a support assembly arranged in the vacuum tank for supporting industrial hemp plants.

2. The industrial hemp pollen tube pathway genetic transformation vacuum apparatus of claim 1, wherein, The support assembly comprises: a base detachably mounted in the vacuum tank; a support rod mounted on the top of the base; and a support plate and a flowerpot clamping plate detachably mounted on the support rod for stabilizing the industrial hemp plant pot, the flowerpot clamping plate being located above the support plate and being in contact with the pot bottom of the industrial hemp plant pot.

3. The industrial hemp pollen tube pathway genetic transformation vacuum apparatus of claim 2, wherein, The flowerpot clamping plate is movably sleeved on the outside of the support rod, a limiting bolt in contact with the support rod is threadedly mounted on the flowerpot clamping plate, a clamping hoop clamped on the outside of the support rod is mounted on the support plate, and the limiting bolt and the clamping hoop are respectively used for stabilizing the flowerpot clamping plate and the support plate.

4. The industrial hemp pollen tube pathway genetic transformation vacuum apparatus of claim 2, wherein, A clamping plate is mounted on the inner wall of the bottom of the vacuum tank, the clamping plate is L-shaped and clamped on the outside of the base, an opening allowing the industrial hemp plants to pass through is arranged on the support plate, and a protective structure for reducing the opening space is detachably mounted on the support plate.

5. The industrial hemp pollen tube pathway genetic transformation vacuum apparatus of claim 4, wherein, The protective structure comprises a connecting frame detachably mounted on the support plate and a separation plate fixed on the connecting frame and extending into the opening for blocking the opening, and the separation plate is used for reducing the leakage amount of the industrial hemp plant soil.

6. The industrial hemp pollen tube channel genetic transformation vacuum apparatus of claim 1, wherein, Extension plates are arranged on the vacuum tank and the PMMA cover plate, the nut bolt is threadedly mounted on any one of the extension plates and is threadedly connected with the other extension plate, and a groove for accommodating the sealing rubber ring is arranged on the top of the vacuum tank.

7. The industrial hemp pollen tube channel genetic transformation vacuum apparatus of claim 1, wherein, The number of the air nozzles is not less than two and the air nozzles are respectively used for air intake and air exhaust, a threaded cap for closing the air nozzle for air intake is sleeved on the air nozzle, the air nozzle for air exhaust is connected with the air intake end of the vacuum pump, and a one-way valve is arranged on the air nozzle.

8. The industrial hemp pollen tube channel genetic transformation vacuum apparatus of claim 1, wherein, Handles for providing gripping points are arranged on both sides of the vacuum tank, a support seat for supporting the vacuum tank is arranged on the bottom of the vacuum tank, and a recess for stabilizing the vacuum tank is arranged in the support seat.

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