Gene chip hybridization temperature control device

Abstract

The utility model relates to the field of chip production and processing equipment. The gene chip hybridization temperature control device comprises a chip container and further comprises a heating bottom plate, the heating bottom plate comprises a vapor chamber and a heater, the heater is installed below the vapor chamber, and the chip container is detachably connected to the upper portion of the vapor chamber; the chip container comprises a chip placing frame, a groove body with an opening in the upper end and a cover body, the upper end and the lower end of the chip placing frame are open, the opening in the upper end and the opening in the lower end of the chip placing frame are communicated with each other, the bottom of the groove body abuts against the opening in the lower end of the chip placing frame, and the cover body abuts against the opening in the upper end of the chip placing frame; a temperature measuring gap for measuring the temperature of liquid in the groove body is formed between the chip placing frame and the groove body; and the temperature measuring mechanism is used for being inserted into the temperature measuring gap. According to the utility model, the chip container is improved, the combination of the chip placing rack, the groove body and the cover body is adopted, and the temperature measuring gap is reserved, so that the hybridization reaction temperature can be conveniently detected.

Description

TECHNICAL FIELD

[0001] The utility model relates to chip production and processing equipment field, concretely relates to temperature control device. BACKGROUND

[0002] The hybridization reaction needs to be carried out in the gene chip sequencing process. At present, when the hybridization reaction of the gene chip is carried out in the laboratory, the sealed metal box is used as the chip container, and the molecular hybridization box with heating function is used to heat the hybridization solution. The hybridization solution temperature in the sealed metal box is maintained by heating inside the molecular hybridization box. This heating method inside the molecular hybridization box has the following defects:

[0003] Although the molecular hybridization box has temperature detection function, the detected temperature is the internal temperature of the molecular hybridization box, which is different from the hybridization solution temperature in the metal box, so it is impossible to accurately control the real-time temperature of the hybridization solution in the metal box. CONTENT OF THE UTILITY MODEL

[0004] In view of the problems existing in the prior art, the utility model provides a gene chip hybridization temperature control device, which has solved at least one of the above technical problems.

[0005] The technical scheme of the utility model is as follows: a gene chip hybridization temperature control device, comprising a chip container, characterized in that it further comprises a heating bottom plate, the heating bottom plate comprises a heat plate and a heater, the heater is installed below the heat plate, and the chip container is detachably connected above the heat plate.

[0006] The chip container comprises a chip placing rack, a groove with an upper opening and a cover, the chip placing rack is open at both upper and lower ends, the upper opening and the lower opening of the chip placing rack are communicated with each other, the bottom of the groove abuts against the lower opening of the chip placing rack, and the cover abuts against the upper opening of the chip placing rack.

[0007] A temperature measurement gap for measuring the temperature of the liquid in the groove is formed between the chip placing rack and the groove.

[0008] The temperature measurement mechanism is used for inserting into the temperature measurement gap.

[0009] The utility model improves the chip container, adopts the combination of the chip placing rack, the groove and the cover, leaves the temperature measurement gap, and facilitates the detection of the hybridization reaction temperature. At the same time, the cover can be easily opened, and the gene chip can be easily observed.

[0010] Further preferably, longitudinal notches are formed in the adjacent two corner portions of the chip placing rack, the notches and the groove form the temperature measurement gap.

[0011] The other two adjacent corner portions of the chip placing rack are connected with a hand-held rack.

[0012] Convenient temperature measurement and taking operation.

[0013] Further preferably, the hand-held frame comprises two vertically arranged columns and a connecting handle connecting the two columns, the connecting handle being provided with an outwardly extending extension.

[0014] Convenient taking.

[0015] Further preferably, a waist-shaped hole is formed in the extension.

[0016] Further preferably, the heating base plate further comprises a support plate, and the heater is clamped between the support plate and the vapor chamber;

[0017] Further comprising a support frame, the support frame being detachably connected with the support plate;

[0018] A moving frame is longitudinally slidably connected on the support frame, and the temperature measurement mechanism is detachably connected on the moving frame.

[0019] Convenient adjustment of the height of the temperature measurement mechanism.

[0020] Further preferably, two chip containers are detachably connected on the vapor chamber;

[0021] The support frame is installed between the two chip containers.

[0022] Further preferably, a U-shaped notch is formed in the vapor chamber to avoid the support frame.

[0023] Further preferably, an upwardly extending rim is arranged on the support plate, the rim and the vapor chamber surrounding the heater to form a cavity accommodating the heater, and at least one heater is arranged in the cavity.

[0024] Further preferably, at least three insertion slots for longitudinally inserting gene chips are formed side by side on the chip placing frame;

[0025] The bottom of the insertion slot is higher than the bottom of the chip placing frame.

[0026] And the insertion slot is in open communication with the lower end opening of the chip placing frame.

[0027] Convenient hybridization treatment of the chip.

[0028] Further preferably, outwardly extending bosses are arranged on opposite sides of the groove body, and mounting holes are formed in the bosses;

[0029] The bosses are detachably connected with the vapor chamber through screws passing through the mounting holes.

[0030] Beneficial effects:

[0031] 1)Optimize chip container, open design, can view gene chip in real time.

[0032] 2) The setting of the temperature measurement gap can measure the real-time temperature of the hybridization solution. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 It is a structural schematic view of a specific embodiment 1 of the utility model;

[0034] Figure 2 It is an exploded view of the heating bottom plate of the specific embodiment 1 of the utility model;

[0035] Figure 3 It is a structural schematic view of the heating bottom plate of the specific embodiment 1 of the utility model;

[0036] Figure 4 It is a structural schematic view of the groove body of the specific embodiment 1 of the utility model;

[0037] Figure 5 It is a structural schematic view of the chip placing rack of the specific embodiment 1 of the utility model.

[0038] In the drawing: 1 is groove body, 2 is chip placing rack, 3 is cover body, 4 is heat plate, 5 is heater, 6 is support plate, 7 is mounting frame, 8 is temperature measurement mechanism. DETAILED DESCRIPTION

[0039] Referring to Figures 1 to 5 , specific embodiment 1, gene chip hybridization temperature control device, including chip container, still including heating bottom plate, heating bottom plate includes heat plate 4 and heater 5, the heater 5 is installed below heat plate 4, and the heat plate 4 is detachably connected with chip container above;Chip container includes chip placing rack 2, groove 1 with upper end opening and cover body 3, the upper end opening and the lower end opening of chip placing rack 2 are mutually communicated, the bottom of groove 1 is against the lower end opening of chip placing rack 2, and the upper end opening of chip placing rack 2 is against cover body 3;Chip placing rack 2 and groove 1 have temperature measurement gap for measuring the temperature of liquid in groove 1;Still include temperature measurement mechanism 8, temperature measurement mechanism 8 is used for inserting temperature measurement gap.The utility model discloses through the improvement of chip container, adopts the combination of chip placing rack 2, groove 1 and cover body 3, leaves temperature measurement gap, and the detection of hybridization reaction temperature is facilitated.

[0040] The material of groove and chip placing rack is SUS316L.Under the premise of saving cost, the service life of container under hybridization solution environment is as long as possible.

[0041] Temperature measurement mechanism 8 can be PT1000 temperature sensor.

[0042] Two adjacent corners of the chip placing rack 2 are provided with longitudinal through gaps, and the gaps and the groove body 1 form a temperature measuring gap; the other two adjacent corners of the chip placing rack 2 are connected with a hand holding rack. The hand holding rack facilitates temperature measurement and taking operation. The hand holding rack comprises two vertical columns and a connecting handle connecting the two columns, and the connecting handle is provided with an outwardly extending extension. The extension facilitates taking. A waist-shaped hole is formed in the extension.

[0043] The heating base plate further comprises a support plate 6, a heater 5 is clamped between the support plate 6 and the heat plate 4; and a support frame 7, which is detachably connected with the support plate 6; a moving frame is longitudinally slidably connected with the support frame 7, and a temperature measuring mechanism 8 is detachably connected with the moving frame. The height of the temperature measuring mechanism can be adjusted.

[0044] Two chip containers are detachably connected with the heat plate 4; and the support frame 7 is installed between the two chip containers. A U-shaped gap is formed in the heat plate 4 to avoid the support frame. An upwardly extending surrounding edge is arranged on the support plate, the surrounding edge and the heat plate form a cavity for accommodating the heater, and at least one heater is arranged in the cavity. The number of the heaters in the cavity is matched with the number of the chip placing racks. Preferably, two heaters are arranged. The heaters are located directly below the chip placing racks.

[0045] At least three insertion slots are formed in the chip placing rack 2 in parallel and used for longitudinally inserting the gene chip; the bottom of the insertion slot is higher than the bottom of the chip placing rack 2; and the insertion slot is in open connection with the lower end opening of the chip placing rack 2. The hybridization treatment of the chip is facilitated.

[0046] The opposite sides of the groove body 1 are provided with outwardly extending bosses, mounting holes are formed in the bosses, and the bosses are detachably connected with the heat plate 4 through screws penetrating the mounting holes.

[0047] In the specific embodiment 2, on the basis of the specific embodiment 1, a support seat is connected below the heating base plate, and a temperature controller is installed on the support seat. The temperature data of the hybridization solution in the chip container is transmitted to the temperature controller in real time through the temperature probe, the temperature controller adjusts the voltage loaded on the heater according to the real-time temperature, controls the heating power of the heater, and the heat plate transmits the heat generated by the heater to the area to be heated. The heater can be a semiconductor refrigeration sheet. The heating surface of the semiconductor refrigeration sheet is tightly attached to the heat plate.

[0048] The above is only the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled persons in the technical field, some improvements and refinements can be made without departing from the principle of the present application, and these improvements and refinements should also be regarded as the protection range of the present application.

Claims

1. A device for temperature control of gene chip hybridization, comprising a chip container, characterized in that, The heating base plate comprises a heat plate and a heater, the heater is mounted below the heat plate, and the chip container is detachably connected above the heat plate; The chip container comprises a chip placing rack, a slot body with an open upper end, and a cover body, both upper and lower ends of the chip placing rack are open, the upper end opening of the chip placing rack is in communication with the lower end opening, the bottom of the slot body abuts against the lower end opening of the chip placing rack, and the cover body abuts against the upper end opening of the chip placing rack; A temperature measurement gap for measuring the temperature of the liquid in the slot body is formed between the chip placing rack and the slot body; The temperature measurement mechanism is used for being inserted into the temperature measurement gap.

2. The gene chip hybridization temperature control device according to claim 1, wherein: Longitudinally extending notches are formed in adjacent two corner portions of the chip placing rack, and the notches and the slot body form the temperature measurement gap; The other two adjacent corner portions of the chip placing rack are connected with a hand holding rack.

3. The gene chip hybridization temperature control device according to claim 2, wherein: The hand holding rack comprises two vertical columns and a connecting handle connecting the two columns, and the connecting handle is provided with an extension extending outward.

4. The gene chip hybridization temperature control apparatus according to claim 3, wherein: A waist-shaped hole is formed in the extension.

5. The gene chip hybridization temperature control apparatus according to claim 1, wherein: The heating base plate further comprises a support plate, and the heater is clamped between the support plate and the heat plate; A support rack is further provided, and the support rack is detachably connected with the support plate; A moving rack is longitudinally and slidingly connected on the support rack, and the temperature measurement mechanism is detachably connected on the moving rack.

6. The gene chip hybridization temperature control apparatus according to claim 5, wherein: Two chip containers are detachably connected on the heat plate; The support rack is mounted between the two chip containers.

7. The gene chip hybridization temperature control apparatus according to claim 6, wherein: A U-shaped notch is formed in the heat plate to avoid the support rack.

8. The gene chip hybridization temperature control apparatus according to claim 5, wherein: An upward extending surrounding edge is provided on the support plate, the surrounding edge and the heat plate form a cavity for accommodating the heater, and at least one heater is arranged in the cavity.

9. The gene chip hybridization temperature control apparatus according to claim 1, wherein: At least three insertion slots for longitudinally inserting gene chips are formed in the chip placing rack in parallel; The bottom of the insertion slot is higher than the bottom of the chip placing rack; The insertion slot is in communication with the lower end opening of the chip placing rack.

10. The gene chip hybridization temperature control apparatus according to claim 1, wherein: Outward extending bosses are provided on opposite sides of the slot body, and mounting holes are formed in the bosses; The bosses are detachably connected with the heat plate through screws passing through the mounting holes.

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