Kudzuvine root exosome separation and purification device

Abstract

The utility model discloses a radix puerariae exosome separation and purification device. The device comprises a main body assembly, wherein the main body assembly comprises a support frame, a mounting frame and a cylinder body; the mounting frame is rotationally connected to the top end of the supporting frame, and the barrel is fixedly connected to the inner side wall of the mounting frame; the cleaning assembly comprises a pulling plate, a clamping ring, a clamping block, a motor and a liquid storage cylinder; the pulling plate is installed at the bottom end of the barrel, the clamping ring is rotationally connected to the outer side wall of the pulling plate, the clamping block is fixedly connected to the bottom wall of the supporting frame, the motor is fixedly connected to the center of the inner wall of the pulling plate, the liquid storage barrel is fixedly connected to one end of the motor, and the cleaning assembly further comprises a filtering barrel, a spring block, a movable groove, a fixing ring and a clamping groove. And the filter cartridge is mounted in the liquid storage cartridge. The problems that most filtering assemblies of an existing pueraria root exosome separation and purification device are arranged in centrifugal equipment, so that the filtering assemblies are troublesome to take out in the later period, and inconvenience is brought to the next purification work are solved.

Description

Technical Field

[0001] This utility model relates to the field of kudzu exosome separation and purification technology, and in particular to a kudzu exosome separation and purification device. Background Technology

[0002] Kudzu root, the dried root of the legume *Pueraria lobata*, is a traditional Chinese medicine used to dispel wind and heat. The main function of the exosome separation and purification device is to extract and purify exosomes from complex biological samples. Exosomes are small membrane vesicles with diameters between 30 and 150 nanometers, containing complex RNA and proteins, and participating in intercellular communication and various physiological and pathological processes. The separation and purification device effectively extracts high-purity exosomes using various techniques, such as size exclusion, density gradient centrifugation, and immunoaffinity.

[0003] In existing kudzu root exosome separation and purification devices, the filter components are mostly located inside the centrifuge equipment, which makes it more troublesome to remove them later and causes inconvenience for the next purification work. Utility Model Content

[0004] The purpose of this invention is to provide a kudzu root exosome separation and purification device to solve the problem mentioned in the background art that the filter components of existing kudzu root exosome separation and purification devices are mostly located inside the centrifuge equipment, which makes it more troublesome to remove them later and causes inconvenience to the next purification work.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0006] This utility model relates to a device for separating and purifying kudzu root exosomes, comprising:

[0007] Main components: The main components include a support frame, a mounting frame, and a cylindrical body;

[0008] The mounting frame is rotatably connected to the top of the support frame, and the cylinder is fixedly connected to the inner wall of the mounting frame.

[0009] Cleaning assembly: The cleaning assembly includes a pull plate, a retaining ring, a retaining block, a motor, and a liquid storage tank;

[0010] The pull plate is installed at the bottom of the cylinder, the retaining ring is rotatably connected to the outer wall of the pull plate, the retaining block is fixedly connected to the bottom wall of the support frame, the motor is fixedly connected to the center of the inner wall of the pull plate, and the liquid storage cylinder is fixedly connected to one end of the motor.

[0011] Furthermore, the cleaning assembly also includes a filter cylinder, a spring block, a movable groove, a retaining ring, and a retaining groove;

[0012] The filter cartridge is installed inside the liquid storage cylinder, the fixed ring is fixedly connected to the top of the liquid storage cylinder, the movable groove is opened on the outside of the fixed ring, the spring block is slidably connected to the inside of the movable groove, and the slot is opened on the inner wall of the top of the liquid storage cylinder.

[0013] Furthermore, the output shaft of the motor is fixedly connected to a liquid storage tank via a coupling, and the inner bottom wall of the liquid storage tank is provided with an arc slope.

[0014] Furthermore, the spring block and the slot are arranged on the same central axis, and the retaining ring and the retaining block are engaged.

[0015] Furthermore, the main component also includes a drain pipe, a centrifuge cylinder, and a sealing cap;

[0016] The drain pipe is fixedly connected to the outer wall of the cylinder, the centrifuge cylinder is fixedly connected to the top of the cylinder, and the sealing cap is fixedly connected to the top of the centrifuge cylinder.

[0017] Furthermore, it also includes the disassembly and assembly of components;

[0018] The assembly / disassembly assembly includes a groove, a rotating plate, and a retaining plate;

[0019] The groove is formed at one end of the support frame, the rotating plate is rotatably connected to the inner wall of the groove, and the clamping plate is fixedly connected to the inner wall of one end of the cylinder.

[0020] Furthermore, the assembly / disassembly assembly also includes a rotating rod;

[0021] The rotating rod is installed at the connection between the mounting frame and the support frame.

[0022] Compared with existing technologies, the advantages of this utility model are:

[0023] This utility model, by setting up a cleaning component and through the auxiliary cooperation between the motor and the liquid storage tank, can release the locking installation between the liquid storage tank and the filter cylinder by the sliding spring block, which can assist the staff in taking out the filter cylinder for cleaning and facilitate the staff in cleaning up residue.

[0024] Based on the aforementioned beneficial effects, a disassembly and assembly component is provided. Through the auxiliary cooperation between the groove and the rotating plate, the mounting frame can be flipped to disassemble and assemble the structure inside the cylinder. This can assist workers in disassembling and assembling the installed structure inside the cylinder, achieving the effect of assisted disassembly and assembly. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is an axonometric view of the present invention;

[0027] Figure 2 This is another axonometric view of the present invention;

[0028] Figure 3 This is an exploded view of the present invention;

[0029] Figure 4 This is an exploded view of the cleaning component of this utility model.

[0030] The attached diagram lists the components represented by each number as follows:

[0031] 11. Support frame; 12. Mounting frame; 13. Cylinder body; 14. Drain pipe; 15. Centrifuge cylinder; 16. Sealing cap;

[0032] 21. Pull plate; 22. Snap ring; 23. Snap block; 24. Motor; 25. Liquid storage tank; 26. Filter cartridge; 27. Spring block; 28. Movable groove; 29. ​​Fixed ring; 210. Snap groove;

[0033] 31. Groove; 32. Rotating plate; 33. Clamping plate; 34. Rotating rod. Detailed Implementation

[0034] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0035] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0036] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in further detail below with reference to the accompanying drawings.

[0037] Please see Figure 1-4 As shown, this embodiment is a device for separating and purifying kudzu exosomes, comprising:

[0038] Main components: The main components include a support frame 11, a mounting frame 12, and a cylinder 13;

[0039] The mounting bracket 12 is rotatably connected to the top of the support frame 11, and the cylinder 13 is fixedly connected to the inner wall of the mounting bracket 12.

[0040] The main components also include a drain pipe 14, a centrifuge cylinder 15, and a sealing cap 16;

[0041] The drain pipe 14 is fixedly connected to the outer wall of the cylinder 13, the centrifuge cylinder 15 is fixedly connected to the top of the cylinder 13, and the sealing cap 16 is fixedly connected to the top of the centrifuge cylinder 15.

[0042] Mounting bracket 12 provides space for mounting cylinder 13, centrifuge cylinder 15 and sealing cap 16. Centrifuge cylinder 15 is used to separate kudzu root, sealing cap 16 is used to increase the sealing of centrifuge cylinder 15, and drain pipe 14 is used to drain secreted liquid.

[0043] All of the above parts are existing technologies;

[0044] Cleaning assembly: The cleaning assembly includes a pull plate 21, a retaining ring 22, a retaining block 23, a motor 24, and a liquid storage tank 25;

[0045] Pull plate 21 is installed at the bottom end of cylinder 13, retaining ring 22 is rotatably connected to the outer wall of pull plate 21, retaining block 23 is fixedly connected to the bottom wall of support frame 11, motor 24 is fixedly connected to the center of inner wall of pull plate 21, and liquid storage cylinder 25 is fixedly connected to one end of motor 24; the cleaning assembly also includes filter cylinder 26, spring block 27, movable groove 28, fixed ring 29 and retaining groove 210;

[0046] The filter cartridge 26 is installed inside the liquid storage cylinder 25, the fixing ring 29 is fixedly connected to the top of the liquid storage cylinder 25, the movable groove 28 is opened on the outside of the fixing ring 29, the spring block 27 is slidably connected to the inside of the movable groove 28, and the slot 210 is opened on the inner wall of the top of the liquid storage cylinder 25.

[0047] The liquid storage cylinder 25 is used to open the slot 210, which provides space for the spring block 27 to engage. The fixed ring 29 is used to open the movable slot 28, which provides space for the spring block 27 to slide. The output shaft of the motor 24 is fixedly connected to the liquid storage cylinder 25 through a coupling.

[0048] With the auxiliary cooperation between the motor 24 and the liquid storage tank 25, the locking installation between the liquid storage tank 25 and the filter tank 26 can be released by the sliding spring block 27.

[0049] Working principle: When staff disassemble and clean the device;

[0050] First, the staff flips the outer retaining ring 22 of the pull plate 21 on one side to release the locking connection between the retaining ring 22 and the retaining block 23. At the same time, the pull plate 21 is pulled outward and the spring block 27 inside the movable groove 28 is slid in sequence to release the limiting engagement between the spring block 27 and the groove 210.

[0051] Next, the filter cartridge 26, with the fixing ring 29 fixedly connected to the top, can be removed from the inside of the liquid storage cylinder 25;

[0052] This step can help staff remove and clean the filter cartridge 26.

[0053] Please see Figure 1-4 As shown, this embodiment, based on the above embodiment, also includes a disassembly and assembly component;

[0054] The assembly and disassembly components include a groove 31, a rotating plate 32, and a retaining plate 33;

[0055] A groove 31 is formed at one end of the support frame 11, a rotating plate 32 is rotatably connected to the inner wall of the groove 31, and a clamping plate 33 is fixedly connected to the inner wall of one end of the cylinder 13.

[0056] The assembly and disassembly components also include a rotating rod 34;

[0057] The rotating rod 34 is installed at the connection between the mounting bracket 12 and the support bracket 11;

[0058] The groove 31 is used to provide space for the rotating plate 32 to rotate. The rotating plate 32 and the clamping plate 33 are set on the same central axis. The rotating rod 34 is used to flip the cylinder 13.

[0059] With the auxiliary cooperation between the groove 31 and the rotating plate 32, the mounting frame 12 can be flipped to disassemble and assemble the structure inside the cylinder 13;

[0060] Working principle: When workers disassemble and assemble the internal structure of the device;

[0061] First, by horizontally flipping the mounting bracket 12 at the top of the support frame 11, and by installing a rotating rod 34 at the connection between the mounting bracket 12 and the support frame 11, the mounting bracket 12 can be flipped to a horizontal plane.

[0062] Next, by flipping the rotating plate 32 inside the groove 31 upwards, the top of the rotating plate 32 can be engaged with the locking plate 33 on the inner side of one end of the mounting bracket 12.

[0063] This step can assist staff in disassembling and reassembling the internal components of cylinder 13.

[0064] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0065] Finally, it should be noted that the above are merely preferred embodiments of this utility model and are not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A device for separating and purifying kudzu root exosomes, characterized in that, include: Main components: The main components include a support frame (11), a mounting frame (12), and a cylinder (13); The mounting bracket (12) is rotatably connected to the top of the support frame (11), and the cylinder (13) is fixedly connected to the inner wall of the mounting bracket (12); Cleaning assembly: The cleaning assembly includes a pull plate (21), a retaining ring (22), a retaining block (23), a motor (24), and a liquid storage tank (25); The pull plate (21) is installed at the bottom of the cylinder (13), the retaining ring (22) is rotatably connected to the outer wall of the pull plate (21), the retaining block (23) is fixedly connected to the bottom wall of the support frame (11), the motor (24) is fixedly connected to the center of the inner wall of the pull plate (21), and the liquid storage cylinder (25) is fixedly connected to one end of the motor (24).

2. The apparatus for separating and purifying kudzu root exosomes according to claim 1, characterized in that, The cleaning assembly also includes a filter cartridge (26), a spring block (27), a movable groove (28), a retaining ring (29), and a retaining groove (210); The filter cylinder (26) is installed inside the liquid storage cylinder (25), the fixing ring (29) is fixedly connected to the top of the liquid storage cylinder (25), the movable groove (28) is opened on the outside of the fixing ring (29), the spring block (27) is slidably connected to the inside of the movable groove (28), and the slot (210) is opened on the inner wall of the top of the liquid storage cylinder (25).

3. The apparatus for separating and purifying kudzu exosomes according to claim 1, characterized in that, The output shaft of the motor (24) is fixedly connected to the liquid storage cylinder (25) via a coupling, and the inner bottom wall of the liquid storage cylinder (25) is provided with an arc slope.

4. The apparatus for separating and purifying kudzu root exosomes according to claim 2, characterized in that, The spring block (27) and the slot (210) are set on the same central axis, and the retaining ring (22) and the retaining block (23) are engaged.

5. The apparatus for separating and purifying kudzu exosomes according to claim 1, characterized in that, The main component also includes a drain pipe (14), a centrifuge tube (15), and a sealing cap (16); The drain pipe (14) is fixedly connected to the outer wall of the cylinder (13), the centrifuge cylinder (15) is fixedly connected to the top of the cylinder (13), and the sealing cap (16) is fixedly connected to the top of the centrifuge cylinder (15).

6. The apparatus for separating and purifying kudzu exosomes according to claim 1, characterized in that, It also includes disassembly and assembly components; The assembly / disassembly assembly includes a groove (31), a rotating plate (32), and a retaining plate (33); The groove (31) is opened at one end of the support frame (11), the rotating plate (32) is rotatably connected to the inner wall of the groove (31), and the clamping plate (33) is fixedly connected to the inner wall of one end of the cylinder (13).

7. The apparatus for separating and purifying kudzu exosomes according to claim 6, characterized in that, The assembly and disassembly assembly also includes a rotating rod (34); The rotating rod (34) is installed at the connection between the mounting frame (12) and the support frame (11).

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