Whole blood RNA preservation tube

Abstract

The utility model discloses a whole blood RNA preservation tube which comprises a preservation tube body and a tube cover, a clamping through hole is fixedly installed in the preservation tube body, a rubber cushion is fixedly installed in the tube cover, an inner sleeve is fixedly installed at the bottom of the tube cover, an outer sleeve is installed on the outer side of the inner sleeve in a sleeved mode, and a clamping through hole is formed in the bottom of the outer sleeve. A guide plate is fixedly mounted at the bottom of the tube cover, a guide hole is formed in the end, away from the tube cover, of the guide plate, a spring is fixedly mounted at the bottom of the tube cover, a connecting plate is fixedly mounted at the end, away from the tube cover, of the spring, a pricking needle is fixedly mounted at the bottom of the connecting plate, and a connecting pull rope is fixedly mounted at the top of the pricking needle. The thin film is arranged at the bottom of the preservation tube body, an independent preservation liquid storage cavity is achieved, preservation liquid is isolated from blood, the human body is prevented from being poisoned by the preservation liquid in the blood sampling process, after blood sampling is completed, the preservation liquid is released through mechanical triggering and is fully mixed with the blood, and RNA stability is ensured.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically a whole blood RNA preservation tube. Background Technology

[0002] RNA is a vital molecule for life processes, and its stability is crucial for gene expression research, clinical diagnosis, and drug development. However, due to the presence of a readily hydrolyzable 2'-hydroxyl group in its molecular structure, RNA is highly susceptible to degradation by RNase enzymes and rapid inactivation due to factors such as temperature and pH after collection. Therefore, developing high-performance RNA preservation tubes is of paramount importance for the effective preservation of RNA in blood samples.

[0003] Current methods for preserving RNA in blood typically involve collecting blood from blood collection tubes and then transferring the blood into RNA preservation tubes. This not only increases the complexity of the procedure but can also lead to RNA degradation or sample contamination. Some commercially available RNA preservation tubes that can be used directly for blood collection do not separate the RNA stabilizing agent from the human body. Because components of the RNA preservation agent are toxic to humans, these tubes cannot completely prevent the preservation agent from coming into contact with the body during blood collection. Therefore, there is an urgent need for a whole blood RNA preservation tube that releases the preservation solution only after blood collection is complete, thus addressing the shortcomings of existing technologies. Utility Model Content

[0004] The purpose of this invention is to provide a whole blood RNA preservation tube to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a whole blood RNA preservation tube, comprising a preservation tube body and a tube cap, wherein a snap-fit ​​through hole is fixedly installed inside the preservation tube body, a rubber pad is fixedly installed inside the tube cap, an inner sleeve is fixedly installed at the bottom of the tube cap, an outer sleeve is sleeved on the outer side of the inner sleeve, a snap-fit ​​through hole is opened at the bottom of the outer sleeve, a guide plate is fixedly installed at the bottom of the tube cap, a guide hole is opened inside the guide plate at the end away from the tube cap, a spring is fixedly installed at the bottom of the tube cap, a connecting plate is fixedly installed at one end of the tube cap on the spring, a needle is fixedly installed at the bottom of the connecting plate, a connecting pull rope is fixedly installed at the top of the needle, and a loop is fixedly installed at the end of the connecting pull rope away from the connecting plate.

[0006] Preferably, the end of the connecting rope away from the connecting plate is located inside the guide hole, and the loop is fitted onto the outside of the outer sleeve.

[0007] Preferably, the outer side of the inner sleeve is provided with four sliding grooves, which are symmetrically arranged on the outer side of the inner sleeve.

[0008] Preferably, a slider is fixedly installed at the top of the inner cavity of the outer sleeve, and there are four sliders, which are symmetrically installed on the inner side of the outer sleeve.

[0009] Preferably, the outer contour of the slider is adapted to the inner contour of the groove, and the four sliders are slidably installed inside the four grooves respectively.

[0010] Preferably, a connecting rod is fixedly installed at the bottom of the tube cap, and a push rope ring is fixedly installed at the end of the connecting rod away from the tube cap. The push rope ring is sleeved on the outside of the outer sleeve, and the push rope ring is located directly above the loop ring.

[0011] Compared with the prior art, the beneficial effects of this utility model are: by setting a thin film at the bottom of the preservation tube to form an independent preservation solution storage chamber, the preservation solution is isolated from the blood, avoiding the toxicity of the preservation solution to the human body during blood collection. After blood collection, the preservation solution is released by mechanical triggering and fully mixed with the blood to ensure RNA stability. After the membrane is broken, the RNA stabilizing solution is quickly mixed with the blood, and the RNA in the sample is protected in a short time, effectively avoiding degradation.

[0012] In addition, the integrated design allows operators to perform only a single blood collection step without the need to add additional preservation solution, saving time and reducing the risk of misoperation. The light-proof design and sealing performance of the tube wall ensure the integrity of the sample during transportation. It can withstand environmental temperature changes from -20℃ to 50℃, ensuring the stability of the sample during long-term transportation. It adopts injection molding process, modular design, and uses medical-grade but low-cost polypropylene (PP) and silicone materials, which are suitable for large-scale industrial production. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural diagram of the present utility model.

[0014] Figure 2 This is a schematic diagram of the internal three-dimensional structure of the storage tube of this utility model.

[0015] Figure 3 This is a three-dimensional structural diagram of the pipe cap of this utility model viewed from below.

[0016] Figure 4 This is a partial three-dimensional structural diagram of the present invention.

[0017] In the diagram: 1. Storage tube body; 2. Tube cap; 3. Rubber pad; 4. Inner sleeve; 5. Slide groove; 6. Connecting rod; 7. Push rope ring; 8. Looping rope ring; 9. Needle; 10. Connecting plate; 11. Connecting pull rope; 12. Spring; 13. Guide hole; 14. Guide plate; 15. Outer sleeve; 16. Sliding block; 17. Snap-fit ​​hole; 18. Diaphragm. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figures 1-4 This utility model provides a technical solution: a whole blood RNA preservation tube, including a preservation tube body 1 and a tube cap 2. The preservation tube body 1 has a locking through hole 17 fixedly installed inside. The tube cap 2 has a rubber pad 3 fixedly installed inside. The bottom of the tube cap 2 has an inner sleeve 4 fixedly installed. The outer sleeve 15 is sleeved on the outer side of the inner sleeve 4. The bottom of the outer sleeve 15 has a locking through hole 17. The bottom of the tube cap 2 has a guide plate 14 fixedly installed. The guide plate 14 has a guide hole 13 on the inner side away from the tube cap 2. The bottom of the tube cap 2 has a spring 12 fixedly installed. The spring 12 is connected to a connecting plate 10 fixedly installed at one end of the tube cap 2. The bottom of the connecting plate 10 has a needle 9 fixedly installed. The top of the needle 9 has a connecting pull rope 11 fixedly installed. The end of the connecting pull rope 11 away from the connecting plate 10 has a loop 8 fixedly installed. The end of the connecting pull rope 11 away from the connecting plate 10 is located inside the guide hole 13. The loop 8 is sleeved on the outer side of the outer sleeve 15.

[0020] The working principle of the above technical solution is as follows: The storage tube 1 is a single-layer tube structure with a length of 100-120mm, an outer diameter of 12-15mm, and an inner diameter of 10-12mm. A membrane 18 is installed inside the tube 1. The upper part of the membrane 18 is used for collecting and storing blood, with a capacity of 5mL. The tube opening is designed with a medical-grade silicone sealing gasket to ensure airtightness. The lower part of the membrane 18 is used to store RNA stabilizing solution, with a storage chamber volume of 3-5mL. The storage chamber is separated from the blood collection area by the membrane. The membrane 18 is made of polyethylene with a thickness of 0.03-0.05mm and can withstand a transport pressure of 1.5atm. During use, the blood collection needle is inserted into the rubber pad 3 inside the tube cap 2, and then the blood collection needle is inserted into the locking hole 17. The blood collection needle continues to move into the storage tube 1, eventually causing the needle body to... The needle is inserted into the through hole 17 and blood is introduced into the blood collection area through vacuum. The maximum collection volume is 5 mL. During blood collection, the spring 12 is compressed, keeping the needle 9 locked. The diaphragm 18 is not broken, and the preservation solution is completely isolated from the blood. After blood collection, the user pulls out the blood collection needle, which moves the outer sleeve 15 upward outside the inner sleeve 4, thereby causing the loop 8 to disengage from the outer sleeve 15. The connecting plate 10 loses the tension of the loop 8 and the connecting rope 11. The needle 9 punctures the diaphragm 18 under the action of the spring 12. The RNA stabilizing solution is released from the preservation chamber through the diaphragm 18 into the blood collection area and mixes thoroughly with the blood. After the blood and preservation solution are mixed, the RNA is effectively protected. The preservation tube 1 has a multi-layer sealing design to ensure airtightness and can be used for transportation and long-term preservation.

[0021] In another implementation scheme, such as Figures 1-4 As shown, the outer side of the inner sleeve 4 is provided with a sliding groove 5. There are four sliding grooves 5, and the four sliding grooves 5 are symmetrically opened on the outer side of the inner sleeve 4. The top of the inner cavity of the outer sleeve 15 is fixedly installed with a slider 16. There are four sliders 16, and the four sliders 16 are symmetrically installed on the inner side of the outer sleeve 15. The outer contour of the slider 16 is adapted to the inner contour of the sliding groove 5. The four sliders 16 are slidably installed in the four sliding grooves 5 respectively.

[0022] When the outer sleeve 15 moves on the outside of the slide groove 5, the slider 16 can move on the inside of the slide groove 5.

[0023] In another implementation scheme, such as Figures 1-4 As shown, a connecting rod 6 is fixedly installed at the bottom of the pipe cover 2. A push rope ring 7 is fixedly installed at the end of the connecting rod 6 away from the pipe cover 2. The push rope ring 7 is sleeved on the outside of the outer sleeve 15 and is located directly above the loop ring 8.

[0024] The push-rope ring 7 can be used to push the rope ring 8 off the outside of the outer sleeve 15 when the outer sleeve 15 moves upward outside the inner sleeve 4.

[0025] Working principle: The storage tube 1 is a single-layer tube structure with a length of 100-120mm, an outer diameter of 12-15mm, and an inner diameter of 10-12mm. A membrane 18 is installed inside 1. The upper part of membrane 18 is used for blood collection and storage, with a capacity of 5mL. The tube opening is designed with a medical-grade silicone sealing gasket to ensure airtightness. The lower part of membrane 18 is used to store RNA stabilizing solution, with a storage chamber volume of 3-5mL. The storage chamber is separated from the blood collection area by the membrane. Membrane 18 is made of polyethylene with a thickness of 0.03-0.05mm and can withstand a transport pressure of 1.5atm. During use, the blood collection needle is inserted into the rubber pad 3 inside the tube cap 2, and then the blood collection needle is inserted into the locking hole 17. The blood collection needle continues to move into the storage tube 1, eventually locking the needle body. Inside the card access hole 17, blood is introduced into the blood collection area through vacuum. The maximum collection volume is 5 mL. During blood collection, the spring 12 is in a compressed state, keeping the needle 9 locked. The diaphragm 18 is not ruptured, and the preservation solution is completely isolated from the blood. After blood collection, the user pulls out the blood collection needle, which drives the outer sleeve 15 to move upward outside the inner sleeve 4, thereby causing the loop 8 to disengage from the outer sleeve 15. The connecting plate 10 loses the tension of the loop 8 and the connecting rope 11. Under the action of the spring 12, the needle 9 punctures the diaphragm 18. The RNA stabilizing solution is released from the preservation chamber through the diaphragm 18 into the blood collection area and mixes thoroughly with the blood. After the blood and preservation solution are mixed, the RNA is effectively protected. The preservation tube 1 ensures airtightness through a multi-layer sealing design and can be used for transportation and long-term preservation.

[0026] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A whole blood RNA preservation tube, comprising a tube body (1) and a cap (2), characterized in that: The storage tube (1) has a snap-fit ​​hole (17) fixedly installed inside. The tube cap (2) has a rubber pad (3) fixedly installed inside. The bottom of the tube cap (2) has an inner sleeve (4) fixedly installed. An outer sleeve (15) is sleeved on the outside of the inner sleeve (4). The bottom of the outer sleeve (15) has a snap-fit ​​hole (17). The bottom of the tube cap (2) has a guide plate (14) fixedly installed. The guide plate (14) has a guide hole (13) inside the end away from the tube cap (2). The bottom of the tube cap (2) has a spring (12) fixedly installed. The end of the spring (12) away from the tube cap (2) has a connecting plate (10) fixedly installed. The bottom of the connecting plate (10) has a needle (9) fixedly installed. The top of the needle (9) has a connecting rope (11) fixedly installed. The end of the connecting rope (11) away from the connecting plate (10) has a rope loop (8) fixedly installed.

2. The whole blood RNA preservation tube according to claim 1, characterized in that: The end of the connecting rope (11) away from the connecting plate (10) is located inside the guide hole (13), and the loop (8) is fitted onto the outside of the outer sleeve (15).

3. A whole blood RNA preservation tube according to claim 2, characterized in that: The inner sleeve (4) has four sliding grooves (5) on its outer side, and the four sliding grooves (5) are symmetrically opened on the outer side of the inner sleeve (4).

4. A whole blood RNA preservation tube according to claim 3, characterized in that: A slider (16) is fixedly installed on the top of the inner cavity of the outer sleeve (15). There are four sliders (16), and the four sliders (16) are symmetrically installed on the inner side of the outer sleeve (15).

5. A whole blood RNA preservation tube according to claim 4, characterized in that: The outer contour of the slider (16) is adapted to the inner contour of the groove (5), and the four sliders (16) are respectively slidably installed inside the four grooves (5).

6. A whole blood RNA preservation tube according to claim 5, characterized in that: A connecting rod (6) is fixedly installed at the bottom of the tube cap (2). A push rope ring (7) is fixedly installed at the end of the connecting rod (6) away from the tube cap (2). The push rope ring (7) is sleeved on the outside of the outer sleeve (15) and is located directly above the loop ring (8).

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