Magnetic bead mixing and cleaning device

Abstract

A magnetic bead mixing and cleaning device effectively solves the problems that magnetic washing and mixing are executed by different units, the occupied space is large, and the space utilization rate is low. Comprising a uniform mixing assembly fixed to the bottom of a magnetic separation pot, a through hole is formed in the bottom of the magnetic separation pot, the uniform mixing assembly comprises a mounting cylinder fixed to the bottom of the magnetic separation pot, the mounting cylinder and the through hole are coaxially arranged, a connecting cylinder is coaxially and rotatably connected to the interior of the mounting cylinder, and a vertically-sliding gripper is coaxially arranged in the connecting cylinder; a connecting block is fixed to the bottom of the connecting cylinder, and connecting pins are fixed to the two sides of the bottom of the connecting block correspondingly. The magnetic cleaning equipment is ingenious in structure and convenient to use, the uniform mixing assembly is arranged at the bottom of the magnetic cleaning equipment through the arrangement of the uniform mixing assembly, the mounting cylinder, the connecting cylinder and the like, a reaction cup is uniformly mixed in the cleaning process, the two execution equipment are integrated, the space utilization rate of the equipment is greatly improved, and the cleaning efficiency of the reaction cup is also greatly improved.

Description

Technical Field

[0001] This utility model relates to the field of in vitro diagnostic equipment technology, and in particular to a magnetic bead mixing and cleaning device. Background Technology

[0002] In the field of in vitro diagnostics, such as chemiluminescence immunoassay analyzers, magnetic washing of reagents and sample reactants is required to remove impurities and extract reactants. During the magnetic washing process, reagents and sample reactants need to be magnetically washed and mixed.

[0003] Chinese patent application number CN202223206804.6 discloses a mixing device for a reaction cup in a chemiluminescence immunoassay analyzer, comprising a load-bearing plate, a drive wheel and a driven wheel rotatably mounted on the upper surface of the load-bearing plate, a synchronous belt tensioned on the drive wheel and the driven wheel, a horizontal plate horizontally mounted on the upper surface of the load-bearing plate via several support columns, a drive turntable and a driven turntable rotatably mounted on the upper surface of the horizontal plate, a drive shaft and a driven shaft respectively eccentrically arranged on the upper surface of the drive turntable and the driven turntable, a mixing cup holder mounted on the drive shaft and the driven shaft, the drive turntable being connected to the driven wheel, and a motor connected to the drive wheel being mounted on the lower surface of the load-bearing plate. The reaction cup mixing device has a simple structure and occupies little space. It consists of a crank-rocker mechanism composed of an active turntable, an active rotating shaft, a mixing cup holder, a driven rotating shaft, and a driven turntable. It utilizes the reciprocating oscillation motion of the crank-rocker mechanism and the quick-return characteristic of the crank-rocker mechanism to make the mixing cup holder perform compound motion in the plane, so that the liquid in the reaction cup is quickly mixed and the effect is significant.

[0004] Chinese patent application CN202321308641.4 discloses a magnetic separation and cleaning device for a chemiluminescence immunoassay analyzer, comprising a rotary disk assembly, a liquid injection assembly, a magnetic bead adsorption assembly, and a liquid suction assembly. A reaction cup is mounted on the rotating end of the rotary disk assembly. The liquid injection and suction assemblies are both mounted on the upper surface of the rotary disk assembly, with their working ends facing the opening of the reaction cup. The magnetic bead adsorption assembly is mounted on the side of the rotary disk assembly, with its working end also facing the side of the reaction cup. This device utilizes double-sided adsorption to fully separate the magnetic beads within the reaction cup, leaving space for liquid suction. Simultaneously, it increases the adsorption force on the magnetic particles within the reaction cup, reducing magnetic loss during waste liquid suction. The three adsorption sections increase the adsorption time of the magnetic beads, and each magnetic adsorption section is connected to the adjacent section. The magnetic particles within the reaction cup are adsorbed on different sides, improving the cleaning effect and the accuracy of the analytical results. This device has an ingenious structure, excellent cleaning and separation effect, high efficiency, and good accuracy.

[0005] Existing magnetic bead mixing and cleaning devices still have the following problems:

[0006] 1. Magnetic washing and mixing are performed by different units, which occupy a large space and have low space utilization.

[0007] 2. Mixing usually involves two actions: linear and rotational. Existing mixing equipment requires two power sources, resulting in high material costs. Utility Model Content

[0008] To address the above problems, this utility model provides a magnetic bead mixing and cleaning device, which has advantages such as small space occupation and low equipment cost.

[0009] The technical solution is as follows: This utility model includes a mixing component fixed to the bottom of a magnetic separation pot. The bottom of the magnetic separation pot has a through hole. The mixing component includes a mounting cylinder fixed to the bottom of the magnetic separation pot. The mounting cylinder is coaxially arranged with the through hole. A connecting cylinder is coaxially rotatably connected inside the mounting cylinder. A gripper that slides up and down is coaxially arranged inside the connecting cylinder. A connecting block is fixed to the bottom of the connecting cylinder. Connecting pins are fixed to both sides of the bottom of the connecting block.

[0010] A connecting plate is provided below the connecting block. Positioning posts corresponding to the connecting pins are fixed on both sides of the top of the connecting plate. The positioning posts and connecting pins have the same diameter. The end of the positioning post furthest from the connecting plate contacts the connecting pin. A rotating sleeve is rotatably connected to the connecting plate. A connecting shaft is coaxially arranged inside the rotating sleeve. The end of the connecting shaft furthest from the connecting plate is coaxially arranged with the gripper. A motor is fixed to the end of the connecting shaft that passes through the connecting plate. A spiral groove is formed through the outer wall of the rotating sleeve. A pin is fixed to the connecting shaft, located within the groove. A limiting plate is fixed to the connecting shaft. Grooves are formed on both sides of the limiting plate, and each groove matches a corresponding positioning post.

[0011] Preferably, the motor is a stepper motor and the number of rotations is an integer.

[0012] Preferably, a movable gap is formed between the positioning post and the connecting pin, and the adjacent ends of the positioning post and the connecting pin are both tapered.

[0013] Preferably, the gripper is composed of multiple circumferentially distributed arc-shaped pieces.

[0014] Preferably, the bottom of the gripper is provided with an insertion hole, the top of the connecting shaft is coaxially fixed with a connecting post that matches the insertion hole, one side of the connecting post is provided with a side plane, one side of the gripper is provided with a positioning hole corresponding to the side plane, a positioning pin is provided in the positioning hole, and one end of the positioning pin is in contact with the side plane.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. By setting up a mixing component, mounting cylinder, and connecting cylinder, the mixing component is placed at the bottom of the magnetic cleaning equipment. During the cleaning process, the reaction cup is mixed. The two execution devices are integrated, which greatly improves the space utilization of the equipment and the cleaning efficiency of the reaction cup is also greatly improved.

[0017] 2. Through the setting of connecting pins, positioning columns, limiting plates and motors, when the limiting plate contacts the positioning column, the rotating sleeve will only drive the connecting shaft to move up and down. When the limiting plate contacts the connecting pin, the limiting plate drives the connecting block to rotate. At this time, the gripper rotates under the drive of the connecting shaft and the connecting cylinder. Only one power source is needed to achieve both linear and rotational changes, which is compatible with the mixing process of the reaction cup. This reduces equipment costs and improves the mixing efficiency of the reaction cup. Attached Figure Description

[0018] Figure 1 This is a perspective view of the present invention.

[0019] Figure 2 This is a structural diagram of the magnetic separation pot in this utility model.

[0020] Figure 3 This is a schematic diagram of the mixing component and the magnetic separation pot in this utility model.

[0021] Figure 4 This is a schematic diagram of the mixing component in this utility model.

[0022] Figure 5 This is a schematic diagram of the gripper moving upwards in this utility model.

[0023] Figure 6 This is a schematic diagram of the rotating sleeve in this utility model.

[0024] Figure 7 This is a schematic diagram of the connecting shaft and gripper in this utility model.

[0025] Figure 8 This is a schematic diagram of the connecting column and connecting parts in this utility model.

[0026] Explanation of the labels in the diagram:

[0027] 1. Magnetic separator; 2. Through hole; 3. Mounting cylinder; 4. Connecting cylinder; 5. Gripper; 6. Connecting block; 7. Connecting pin; 8. Connecting plate; 9. Positioning post; 10. Rotating sleeve; 11. Connecting shaft; 12. Motor; 13. Slide groove; 14. Pin; 15. Limiting plate; 16. Insertion hole; 17. Connecting post; 18. Side plane; 19. Positioning hole. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Depend on Figures 1 to 7 The invention includes a mixing assembly fixed to the bottom of a magnetic separation pot 1, which is a component of a magnetic separation cleaning device. It is mainly used in conjunction with a chemiluminescence immunoassay analyzer. The magnetic separation cleaning removes magnetic particles and other interfering substances from the reaction cup, and then enzyme-catalyzed chemiluminescence substrates (acidic excitation solution and alkaline excitation solution) are added for measurement. In the magnetic separation and cleaning process, the target analyte in the sample is first captured and bound by magnetic particles (usually the main component of the magnetic particle reagent) as a solid-phase carrier. Then, the magnetic particles directly or indirectly bound to the target analyte are collected on the inner wall of the reaction vessel by magnetic force. After multiple injections of cleaning solution and extraction of waste liquid, the free markers and other interfering impurities not bound to the magnetic particles are finally removed to allow for signal measurement of the antigen-antibody conjugates (i.e., magnetic particle conjugates) attached to the magnetic particles. Considering that the existing magnetic cleaning and mixing equipment are performed by different devices, which is not only cumbersome to use but also occupies a large space, resulting in low equipment space utilization, the bottom of the magnetic separation pot 1 is provided with a through hole 2. The mixing component includes a mounting cylinder 3 fixed to the bottom of the magnetic separation pot 1, mainly used for fixing with the magnetic separation pot 1. It should be noted that the mixing component is located in the first cleaning stage of the magnetic separation and cleaning equipment. In the washing process, in this embodiment, the magnetic separation cleaning equipment needs to clean the reaction cup three times. The cleaning steps are basically the same each time. Mixing treatment is required during each cleaning process. During the second and third mixing treatment, the magnetic separation cleaning equipment moves the reaction cup in the opposite direction to the top of the mixing component. The mounting cylinder 3 is coaxially arranged with the through hole 2. The mounting cylinder 3 is coaxially rotatably connected to the connecting cylinder 4. The connecting cylinder 4 is coaxially arranged with a vertically sliding gripper 5 for fixing the reaction cup. The bottom of the connecting cylinder 4 is fixed with a connecting block 6. The bottom sides of the connecting block 6 are respectively fixed with connecting pins 7. By setting up the mixing component, the mounting cylinder 3 and the connecting cylinder 4, the mixing component is set at the bottom of the magnetic cleaning equipment. The reaction cup is mixed during the cleaning process. The integration of the two execution devices greatly improves the space utilization of the equipment and the cleaning efficiency of the reaction cup is also greatly improved.

[0030] Considering that a mixing assembly is needed to mix the reaction cup during cleaning, but mixing usually involves both linear and rotational movements, existing mixing equipment requires two power sources, resulting in high material costs, a connecting plate 8 is provided below the connecting block 6. Positioning posts 9, corresponding to connecting pins 7, are fixed on both sides of the top of the connecting plate 8. The diameters of the positioning posts 9 and connecting pins 7 are the same. The end of the positioning post 9 away from the connecting plate 8 contacts the connecting pin 7. A rotating sleeve 10 is rotatably connected to the connecting plate 8. A connecting shaft 11 is coaxially arranged inside the rotating sleeve 10. The end of the connecting shaft 11 away from the connecting plate 8 is coaxially arranged with the gripper 5. A motor 12 is fixed to one end of the connecting shaft 11 that passes through the connecting plate 8. A mounting plate is provided on the top of the motor 12. The mounting plate is fixed to the bottom of the magnetic separation pot 1. The connecting plate 8 is also fixedly connected to the mounting plate. A spiral groove 13 is formed through the outer wall of the rotating sleeve 10. The connecting shaft 1... A pin 14 is fixed on the connecting shaft 11, and the pin 14 is located in the slide groove 13. A limiting plate 15 is fixed on the connecting shaft 11. The limiting plate 15 has grooves on both sides, and the two grooves are respectively matched with the corresponding positioning posts 9. With the connecting pin 7, positioning posts 9, limiting plate 15 and motor 12, when the motor 12 is started, the rotating sleeve 10 rotates. At this time, the connecting shaft 11 moves upward under the action of the pin 14 and slide groove 13. The gripper 5 moves up and down under the action of the connecting shaft 11. It should be noted that when the limiting plate 15 contacts the positioning post 9, the rotating sleeve 10 only drives the connecting shaft 11 to move up and down. When the limiting plate 15 contacts the connecting pin 7, the limiting plate 15 drives the connecting block 6 to rotate. At this time, the gripper 5 rotates under the action of the connecting shaft 11 and connecting cylinder 4. Only one power source is used to achieve both linear and rotational changes, which is consistent with the mixing process of the reaction cup. It not only reduces the equipment cost, but also improves the mixing efficiency of the reaction cup.

[0031] Considering that the limiting plate 15 needs to be reset after rotation, the motor 12 is a stepper motor and the number of rotations is an integer.

[0032] refer to Figure 4 As shown, in order to prevent the contact surface friction between the positioning post 9 and the connecting pin 7 from being too large when the connecting pin 7 rotates, which would be detrimental to rotation, an movable gap is formed between the positioning post 9 and the connecting pin 7, and the adjacent ends of the positioning post 9 and the connecting pin 7 are both tapered.

[0033] refer to Figure 7 As shown, to further elaborate on the structure of the gripper 5, the gripper 5 is composed of multiple circumferentially distributed arc-shaped pieces, and the reaction cup is inserted into the cylinder composed of multiple arc-shaped pieces. The arc-shaped pieces have a certain degree of elasticity.

[0034] refer to Figure 8As shown, to further supplement the connection method of the gripper 5 and the connecting post 17, in this embodiment, the bottom of the gripper 5 is provided with an insertion hole 16, and the top of the connecting shaft 11 is coaxially fixed with a connecting post 17 that matches the insertion hole 16. A side plane 18 is provided on one side of the connecting post 17, and a positioning hole 19 corresponding to the side plane 18 is provided on one side of the gripper 5. A positioning pin is provided in the positioning hole 19, and one end of the positioning pin contacts the side plane 18. Through the insertion hole 16, the connecting post 17, the side plane 18, and the positioning pin, when the limiting plate 15 contacts the connecting pin 7, the rotation of the connecting shaft 11 is no longer restricted by the positioning post 9. At this time, the gripper 5 rotates under the drive of the connecting shaft 11.

[0035] When using this utility model:

[0036] First, start the motor 12, and the rotating sleeve 10 will rotate accordingly. The connecting shaft 11 will move upward under the action of the pin 14 and the slide 13, and the gripper 5 will move up and down accordingly under the action of the connecting shaft 11.

[0037] Then, it should be noted that when the limiting plate 15 contacts the positioning post 9, the rotating sleeve 10 will only drive the connecting shaft 11 to move up and down. When the limiting plate 15 contacts the connecting pin 7, the limiting plate 15 drives the connecting block 6 to rotate. At this time, the gripper 5 rotates under the drive of the connecting shaft 11 and the connecting cylinder 4.

[0038] Finally, the mixing component is located at the first cleaning step of the magnetic separation cleaning equipment. In this embodiment, the magnetic separation cleaning equipment needs to clean the reaction cup three times. The cleaning steps are basically the same each time. Mixing treatment is required in each cleaning process. When mixing treatment is performed for the second and third time, the magnetic separation cleaning equipment can move the reaction cup in the opposite direction to the top of the mixing component.

[0039] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A magnetic bead mixing and cleaning device, comprising a mixing assembly fixed to the bottom of a magnetic separation pot (1), characterized in that, The bottom of the magnetic separation pot (1) is provided with a through hole (2). The mixing component includes an installation cylinder (3) fixed to the bottom of the magnetic separation pot (1). The installation cylinder (3) is coaxially arranged with the through hole (2). The interior of the installation cylinder (3) is coaxially rotatably connected to a connecting cylinder (4). The connecting cylinder (4) is coaxially arranged with a gripper (5) that slides up and down. The bottom of the connecting cylinder (4) is fixed with a connecting block (6). The bottom sides of the connecting block (6) are respectively fixed with connecting pins (7). A connecting plate (8) is provided below the connecting block (6). Positioning posts (9) corresponding to the connecting pin (7) are fixed on both sides of the top of the connecting plate (8). The positioning posts (9) and the connecting pin (7) have the same diameter. The end of the positioning post (9) away from the connecting plate (8) contacts the connecting pin (7). A rotating sleeve (10) is rotatably connected to the connecting plate (8). A connecting shaft (11) is coaxially arranged inside the rotating sleeve (10). The connecting shaft (11) is located away from the connecting plate (8). One end is coaxially arranged with the gripper (5). The motor (12) is fixed at one end of the connecting shaft (11) that passes through the connecting plate (8). A spiral groove (13) is opened through the outer wall of the rotating sleeve (10). A pin (14) is fixed on the connecting shaft (11). The pin (14) is located in the groove (13). A limit plate (15) is fixed on the connecting shaft (11). Grooves are opened on both sides of the limit plate (15). The two grooves are matched with the corresponding positioning posts (9).

2. The magnetic bead mixing and cleaning device according to claim 1, characterized in that, The motor (12) is a stepper motor and the number of rotations is an integer.

3. The magnetic bead mixing and cleaning device according to claim 2, characterized in that, A movable gap is formed between the positioning post (9) and the connecting pin (7), and the adjacent ends of the positioning post (9) and the connecting pin (7) are both tapered.

4. The magnetic bead mixing and cleaning device according to claim 3, characterized in that, The gripper (5) is composed of multiple circumferentially distributed arc-shaped pieces.

5. The magnetic bead mixing and cleaning device according to claim 4, characterized in that, The gripper (5) has an insertion hole (16) at its bottom. The top of the connecting shaft (11) is coaxially fixed with a connecting post (17) that matches the insertion hole (16). A side plane (18) is provided on one side of the connecting post (17). A positioning hole (19) corresponding to the side plane (18) is provided on one side of the gripper (5). A positioning pin is provided in the positioning hole (19). One end of the positioning pin is in contact with the side plane (18).

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