Rapid propagation device for tissue culture

Abstract

The utility model discloses a rapid propagation device for tissue culture and belongs to the technical field of propagation devices. Comprising an incubator and a bearing frame, the bearing frame is arranged in the incubator, outer rails are symmetrically arranged on the inner wall of the bearing frame, inner rails are arranged on the inner wall, on one sides of the outer rails, of the bearing frame, gaps exist between the outer rails and the inner rails, and multiple sets of connecting columns are arranged between the outer rails and the inner rails at equal intervals; the connecting columns slide between the outer rail and the inner rail, a plurality of sets of connecting rods are arranged outside the outer rail at equal intervals, the connecting rods are movably connected with the connecting columns, a linkage plate is movably arranged between every two sets of connecting columns, and a driving motor is arranged on the outer wall of the incubator. According to the culture tissue taking and placing device, culture tissues can be conveniently taken and placed in a surrounding manner, the culture tissues can be conveniently swung back and forth to uniformly spray a culture solution, and the taking and placing convenience and the culture quality of the culture tissues are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of propagation device, concretely to a tissue culture rapid propagation device. BACKGROUND

[0002] Tissue culture is a new asexual propagation technology developed by using plant cell totipotency theory, and in a broad sense, tissue culture is also called in vitro culture, which refers to separating the tissues, organs or cells meeting the needs from a plant body, and culturing under artificial control conditions through aseptic operation to obtain a regenerated complete plant or produce other products with economic value, and the common tissue culture frame is generally a multi-layer frame body, which is placed in a constant temperature and humidity or other environment set according to requirements, but it is inconvenient to take the culture dish at the high position, in order to improve the situation, a tissue culture rapid propagation device is provided.

[0003] As disclosed in a jujube tissue culture in vitro rapid propagation device with the authorized announcement number CN115136892B, a culture box is provided, the inside of the culture box is fixed with a horizontally placed flat plate, a circular hole is formed in the middle of the flat plate, and a containing assembly for placing and culturing in vitro tissues is arranged in the circular hole, four first springs in cross distribution are fixed on the periphery of the containing assembly, and the four first springs are all fixed with the flat plate, so that the containing assembly is horizontally suspended in the circular hole.

[0004] Although the jujube tissue culture in vitro rapid propagation device with the authorized announcement number CN115136892B realizes that the first annular magnet and the second annular magnet are disconnected when the shaking mechanism is arranged below the flat plate, the bottom disc is lifted by the elastic force of the second spring, so that it is kept inclined, then the motor is started to work, the third rocking rod is driven by the motor and cooperates with the two first rocking rods to circularly push the floating disc to rotate around the bottom surface of the bottom disc, so that the bottom disc is continuously inclined and shaken, the nutrient solution is in full contact with the jujube tissues, and the jujube tissues are beneficial to rapid culture.

[0005] However, the existing propagation device is not convenient for taking and placing the culture tissues in a surrounding mode, is not conducive to uniformly spraying the culture solution by reciprocating and swinging the culture tissues, and affects the convenience of taking and placing the culture tissues and the culture quality. UTILITY MODEL CONTENTS

[0006] The utility model aims at providing a tissue culture rapid propagation device to solve the problem that the propagation device is not convenient for taking and placing the culture tissues in a surrounding mode, is not conducive to uniformly spraying the culture solution by reciprocating and swinging the culture tissues, and affects the convenience of taking and placing the culture tissues and the culture quality.

[0007] In order to achieve the above object, the utility model provides the following technical scheme: a tissue culture rapid propagation device, including incubator and bearing frame, the inside of incubator is provided with bearing frame, the inner wall of bearing frame is provided with outer track symmetry, the inner wall of bearing frame of one side of outer track is all provided with inner track, and there is gap between outer track and inner track, and a plurality of groups of connecting columns of equal interval are arranged between outer track and inner track, and connecting column slides between outer track and inner track, the outside of outer track is all provided with a plurality of groups of connecting rods of equal interval, and connecting rod is movably connected with connecting column, and the movable plate is movably arranged between every two groups of connecting columns, the outer wall of incubator is provided with drive motor, the output end of drive motor is installed transmission shaft, and the surface of transmission shaft is symmetrically sleeved with the stirring plate, and the stirring plate can drive connecting column to move, the inside of bearing frame is provided with a plurality of groups of culture dishes of equal interval, the sidewall of culture dish is all symmetrically installed with connecting frame, the end away from connecting column of movable plate is all provided with movable shaft, and connecting frame is movably connected with movable plate through movable shaft.

[0008] Further, the top end of the incubator is provided with a liquid tank, and the inner wall of the incubator is symmetrically provided with an integrated frame.

[0009] Further, the inside of the integrated frame is movably provided with a reciprocating screw rod, and the surface of the reciprocating screw rod is sleeved with a threaded sleeve.

[0010] Further, the outer wall of the integrated frame is provided with a transmission motor, and the output end of the transmission motor is connected with the reciprocating screw rod.

[0011] Further, the threaded sleeves are provided with a bearing plate, and the bearing plate is connected with the threaded sleeves.

[0012] Further, a servo motor is installed at the center position of the bottom end of the bearing plate, and a gear is installed at the output end of the servo motor.

[0013] Further, a sliding block is slidably installed at the bottom end of the bearing plate on both sides of the servo motor, and a rack is installed on the sidewall of the sliding block, and the rack is meshed with the gear.

[0014] Further, a spraying frame is installed at the bottom end of the sliding block, and the spraying frame is connected with the liquid tank through a flexible hose.

[0015] Compared with the prior art, the beneficial effects of the utility model are that: the propagation device not only realizes the convenient surrounding type taking and placing of culture tissue, facilitates the reciprocating swing of the culture tissue, and uniformly sprays the culture solution, but also improves the convenience of taking and placing the culture tissue and the culture quality.

[0016] By opening the incubator door, a drive motor rotates a lever plate via a transmission shaft. The lever plate moves the connecting column between the outer and inner tracks. The connecting rod and the linkage plate form a circular link, which in turn moves the linkage plate via a movable shaft, causing the connecting frame and culture dishes to move in a circular motion. This moves the culture dishes to the door position, and then the tissues to be cultured are placed inside the culture dishes sequentially. This cultivation method avoids the inconvenience of accessing upper culture dishes in traditional multi-layer culture racks. At the highest point, the water pump inside the liquid tank pumps the culture medium inside the tank through a flexible hose to the inside of the spray frame and sprays it out to irrigate the tissue to be cultured. At the same time, the drive motor is turned on, which drives the reciprocating screw to rotate. The reciprocating screw drives the threaded sleeve to move back and forth, and the threaded sleeve drives the support plate and the spray frame to move back and forth. Thus, the spray frame moves to evenly spray the culture medium onto the cultured tissue, realizing convenient circling loading and unloading of the cultured tissue and facilitating the reciprocating swing to evenly spray the culture medium onto the cultured tissue.

[0017] By turning the servo motor forward and backward, the servo motor drives the gear to rotate forward and backward. The gear drives the rack to swing left and right, and the rack drives the spray frame to swing left and right through the sliding block. This swings left and right to spray the culture medium onto the cultured tissue, thereby improving the uniformity of the culture medium spray and improving the culture quality of the cultured tissue. Attached Figure Description

[0018] The accompanying drawings, which form part of this application, are used to provide a further understanding of the application and to make other features, objects, and advantages of the application more apparent. The illustrative embodiments and descriptions of this application are used to explain the application and do not constitute an undue limitation of the application.

[0019] Furthermore, throughout the accompanying drawings, the same or similar reference numerals denote the same or similar elements. It should be understood that the drawings are schematic, and the elements are not necessarily drawn to scale.

[0020] In the attached diagram:

[0021] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0022] Figure 2 This is a three-dimensional perspective structural diagram of the incubator of this utility model;

[0023] Figure 3 This is a side sectional view of the support frame of this utility model.

[0024] Figure 4 This is a partial three-dimensional structural diagram of the support frame of this utility model;

[0025] Figure 5 It is the bottom view section structure schematic diagram of the integrated frame of the utility model.

[0026] Figure 6 It is the three-dimensional structure schematic diagram of the bearing plate of the utility model.

[0027] Reference signs:

[0028] 1, incubator; 2, liquid tank; 3, integrated frame; 4, bearing frame; 5, drive motor; 6, transmission shaft; 7, dial plate; 8, outer track; 9, connecting rod; 10, connecting column; 11, linkage plate; 12, movable shaft; 13, connecting frame; 14, culture dish; 15, threaded sleeve; 16, reciprocating screw rod; 17, transmission motor; 18, bearing plate; 19, servo motor; 20, gear; 21, spraying frame; 22, sliding block; 23, rack; 24, inner track. DETAILED DESCRIPTION

[0029] Embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although some embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure can be implemented in various forms, and should not be interpreted as being limited to the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough and complete understanding of the present disclosure. It should be understood that the drawings and embodiments of the present disclosure are for exemplary purposes only, and are not intended to limit the scope of protection of the present disclosure.

[0030] In addition, it should be further noted that, for the convenience of description, only the parts related to the application are shown in the drawings. The embodiments in the present disclosure and the features in the embodiments can be combined with each other without conflict.

[0031] It should be noted that the concepts of "first", "second", etc. mentioned in the present disclosure are only used to distinguish different devices, modules or units, and are not used to limit the order or interdependence of the functions performed by these devices, modules or units.

[0032] It should be noted that the modification of "one" or "multiple" in the present disclosure is illustrative but not restrictive, and those skilled in the art should understand that, unless otherwise explicitly indicated in the context, it should be understood as "one or more".

[0033] Please refer to Figures 1 to 6The utility model provides an embodiment: a kind of tissue culture rapid propagation device, including incubator 1 and bearing frame 4, the inside of incubator 1 is provided with bearing frame 4, and the inner wall of bearing frame 4 is symmetrically provided with outer track 8, and the inner wall of bearing frame 4 on the side of outer track 8 is all provided with inner track 24, and gap exists between outer track 8 and inner track 24, and equal-interval multiple groups of connecting columns 10 are arranged between outer track 8 and inner track 24, and connecting column 10 slides between outer track 8 and inner track 24, and the outside of outer track 8 is all provided with equal-interval multiple groups of connecting rod 9, and connecting rod 9 and connecting column 10 are movably connected, and the movable plate 11 is movably arranged between every two connecting columns 10, and the outer wall of incubator 1 is provided with drive motor 5, and drive motor 5 plays the role of power drive, and the output end of drive motor 5 is installed with transmission shaft 6, and the surface of transmission shaft 6 is symmetrically sleeved with the plate 7, and the plate 7 can drive connecting column 10 to move, and the inside of bearing frame 4 is provided with equal-interval multiple groups of culture dish 14, and the sidewall of culture dish 14 is all symmetrically installed with connecting frame 13, and the end of movable plate 11 away from connecting column 10 is all provided with movable shaft 12, and connecting frame 13 is movably connected with movable plate 11 by movable shaft 12.

[0034] The door of incubator 1 is opened, and drive motor 5 is opened, and the plate 7 is rotated by drive motor 5 through transmission shaft 6, and connecting column 10 is moved between outer track 8 and inner track 24 by the plate 7, wherein connecting rod 9 and movable plate 11 form annular link, and then movable plate 11 is moved by connecting column 10, and connecting frame 13 and culture dish 14 are moved by movable plate 11 through movable shaft 12, to move culture dish 14 to the door position respectively, and then the tissue to be cultured is placed in culture dish 14 in sequence, and this culture method avoids the situation that traditional multilayer culture frame is not convenient to take upper culture dish, and when culture dish 14 rotates to the highest point in sequence, the culture solution in liquid tank 2 is pumped into the inside of spraying frame 21 and sprayed by water pump in the inside of liquid tank 2, to irrigate the tissue to be cultured, and transmission motor 17 is opened, and reciprocating screw rod 16 is rotated by transmission motor 17, and reciprocating screw rod 16 drives threaded sleeve 15 to move back and forth under the thread connection of reciprocating screw rod 16 and threaded sleeve 15, and threaded sleeve 15 drives bearing plate 18 and spraying frame 21 to move back and forth, so that spraying frame 21 moves and uniformly sprays culture solution to the culture tissue, to realize convenient annular taking and placing culture tissue, and facilitate reciprocating oscillation and uniform spraying of culture solution to the culture tissue.

[0035] The top of incubator 1 is installed with liquid tank 2, and the inner wall of incubator 1 is symmetrically provided with integrated frame 3, and reciprocating screw rod 16 is movably installed in the inside of integrated frame 3, and the surface of reciprocating screw rod 16 is sleeved with threaded sleeve 15, and threaded sleeve 15 is threadedly connected with reciprocating screw rod 16.

[0036] The outer wall of the integrated frame 3 is provided with a transmission motor 17, which serves as a power drive, and the output end of the transmission motor 17 is connected with the reciprocating screw rod 16. The bearing plate 18 is arranged between the threaded sleeves 15 and connected with the threaded sleeves 15. The servo motor 19 is installed at the bottom center of the bearing plate 18, which serves as a power drive, and the output end of the servo motor 19 is provided with a gear 20.

[0037] The bottom end of the bearing plate 18 on both sides of the servo motor 19 is slidably provided with a sliding block 22, and the side wall of the sliding block 22 is provided with a rack 23, and the rack 23 is engaged with the gear 20.

[0038] The bottom end of the sliding block 22 is provided with a spraying frame 21, and the spraying frame 21 is connected with the liquid tank 2 through a flexible hose.

[0039] Then the servo motor 19 is opened and closed, the gear 20 is driven by the servo motor 19 to rotate forward and reverse, under the mutual engagement of the gear 20 and the rack 23, the rack 23 is driven by the gear 20 to swing left and right, under the sliding cooperation of the sliding block 22 and the bearing plate 18, the spraying frame 21 is driven by the rack 23 through the sliding block 22 to swing left and right, and then the culture solution is sprayed on the culture tissue to further improve the uniformity of the spraying of the culture solution and improve the culture quality of the culture tissue.

[0040] Working principle: open the box door of the incubator 1, the driving motor 5 drives the toggle plate 7 to rotate through the transmission shaft 6, the connecting column 10 is moved between the outer rail 8 and the inner rail 24 by the toggle plate 7, wherein the connecting rod 9 and the linkage plate 11 form a ring-shaped link, and then the linkage plate 11 is moved by the connecting column 10, the connecting frame 13 and the culture dish 14 are moved around by the linkage plate 11 through the movable shaft 12, so as to move the culture dish 14 to the box door position respectively, and then the tissues to be cultured are placed in the culture dish 14 in turn. This culture method avoids the inconvenience of taking the upper culture dish in the traditional multi-layer culture rack. When the culture dish 14 rotates to the highest point in turn, the water pump in the liquid tank 2 pumps the culture solution in the liquid tank 2 to the inside of the spraying frame 21 through the flexible hose and sprays it out, to irrigate the tissue to be cultured. At the same time, the transmission motor 17 is turned on, the reciprocating screw rod 16 is rotated by the transmission motor 17, the threaded sleeve 15 is moved back and forth by the reciprocating screw rod 16 under the threaded connection of the reciprocating screw rod 16 and the threaded sleeve 15, the bearing plate 18 and the spraying frame 21 are moved back and forth by the threaded sleeve 15, so that the spraying frame 21 moves the culture tissue to be sprayed with culture solution. Then the servo motor 19 is opened in both directions, the gear 20 is rotated in both directions by the servo motor 19, the rack 23 is swung left and right by the gear 20 under the mutual engagement of the gear 20 and the rack 23, the spraying frame 21 is swung left and right by the rack 23 through the sliding block 22 under the sliding cooperation of the sliding block 22 and the bearing plate 18, and then the culture tissue is sprayed with culture solution by swinging left and right, to further improve the uniformity of the culture solution spraying, thereby improving the culture quality of the culture tissue. The above is the whole use of the tissue culture rapid propagation device.

[0041] The above description is only some of the preferred embodiments of the present disclosure and the explanation of the technical principles applied. Those skilled in the art should understand that the scope of the application involved in the embodiments of the present disclosure is not limited to the technical solutions formed by the specific combinations of the above technical features, and should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the above inventive concept. For example, the above features are replaced with the technical features disclosed in the embodiments of the present disclosure (but not limited to) having similar functions to form technical solutions.

Claims

1. A tissue culture rapid propagation apparatus comprising a culture cabinet and a carrier rack, characterized in that: The inside of the incubator is provided with a bearing frame, outer rails are symmetrically arranged on the inner wall of the bearing frame, inner rails are arranged on one side of the inner wall of the bearing frame, and there is a gap between the outer rails and the inner rails, and a plurality of groups of connecting columns are arranged at equal intervals between the outer rails and the inner rails, and the connecting columns slide between the outer rails and the inner rails, a plurality of groups of connecting rods are arranged at equal intervals outside the outer rails, and the connecting rods are movably connected with the connecting columns, and a linkage plate is movably arranged between every two groups of connecting columns, a drive motor is arranged on the outer wall of the incubator, a transmission shaft is mounted on the output end of the drive motor, and a driving plate is symmetrically sleeved on the surface of the transmission shaft, and the driving plate can drive the connecting columns to move, a plurality of groups of culture dishes are arranged at equal intervals in the inside of the bearing frame, connecting frames are symmetrically mounted on the side walls of the culture dishes, and movable shafts are arranged at the ends of the linkage plates away from the connecting columns, and the connecting frames are movably connected with the linkage plates through the movable shafts.

2. The tissue culture rapid propagation device of claim 1, wherein: The top end of the incubator is provided with a liquid tank, and the inner wall of the incubator is symmetrically provided with an integrated frame.

3. The tissue culture rapid propagation device of claim 2, wherein: Reciprocal screw rods are movably mounted in the inside of the integrated frame, and threaded sleeves are sleeved on the surfaces of the reciprocal screw rods.

4. The tissue culture rapid propagation apparatus of claim 2, wherein: Driving motors are mounted on the outer walls of the integrated frame, and the output ends of the driving motors are connected with the reciprocal screw rods.

5. The tissue culture rapid propagation device of claim 3, wherein: Bearing plates are arranged between the threaded sleeves, and the bearing plates are connected with the threaded sleeves.

6. The tissue culture rapid propagation device of claim 5, wherein: A servo motor is mounted at the center of the bottom end of the bearing plate, and a gear is mounted on the output end of the servo motor.

7. The tissue culture rapid propagation device of claim 6, wherein: Sliding blocks are slidably mounted at the bottom end of the bearing plate on both sides of the servo motor, and racks are mounted on the side walls of the sliding blocks, and the racks are meshed with the gear.

8. The tissue culture rapid propagation device of claim 7, wherein: Spraying frames are mounted at the bottom end of the sliding blocks, and the spraying frames are connected with the liquid tank through flexible hoses.

Images