Microfluidic chip

Abstract

The micro-fluidic chip comprises a chip main body, and the chip main body is provided with a first sample inlet, a second sample inlet, a third sample inlet, a first detection system, a second detection system, a third detection system, a first driving channel, a second driving channel and a third driving channel. The first detection system is communicated with the first sample inlet and is used for carrying out optical detection on the sample; the second detection system is communicated with the second sample inlet and is used for carrying out optical detection and impedance detection on the sample. The second detection system is communicated with the third sample inlet and is used for carrying out impedance detection on the sample. The first driving channel communicates with the first detection system and is used for driving liquid to run in the first detection system. And the second driving channel is communicated with the second detection system and is used for driving the liquid to run in the second detection system. And the third driving channel is communicated with the third detection system and is used for driving the liquid to run in the third detection system.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of medical apparatus and instruments, especially to a microfluidic chip. BACKGROUND

[0002] Routine blood test is a project that people often need to do when having a physical examination, and the health condition of the examinee is determined by observing the quantity change and morphological distribution of blood cells in the blood sample, so as to further analyze and determine the health condition of the examinee.

[0003] With the development of modernization and automation of detection methods, the routine blood test is generally automatically completed by a detection instrument. Since the volume of red blood cells and platelets is small, it is difficult to accurately detect them by using the optical method as white blood cells. At present, the commonly used method is to dilute the blood sample in the reaction pool of the detection instrument, then count the red blood cells and platelets in the blood sample by using the impedance method, add a certain amount of diluted blood sample to the hemolysis reagent to dissolve the red blood cells, then count and classify the white blood cells in the blood sample by using the impedance method, and determine the hemoglobin by using the colorimetric method, finally, the mixed sample after determination is discharged out of the detection instrument, which is the routine blood test of one blood sample. In addition, all the paths passed by the blood sample need to be cleaned to ensure cleanliness, so as to perform the test on the next blood sample.

[0004] However, since the sampling device, reaction pool, metering pool, quantitative device and fluid pipeline and other components used in the above detection steps are all arranged in the interior of the detection instrument, especially the components related to fluid and liquid chemical reagents are connected to the detection instrument, the existing detection instrument has the problems of high overall cost, large volume, large weight, complex structure and inconvenience for moving, and can only be used in a fixed position in the laboratory or detection room, which has a large use limitation.

[0005] In addition, the above steps of sampling, sample splitting, dilution, mixing, hemolysis, measurement and cleaning are automatically completed by the detection instrument, and any problem in any step will affect the accuracy of the blood cell detection result. When a problem occurs, the detection instrument needs to be shut down and fully investigated to confirm the problem reason, which affects the detection efficiency. UTILITY MODEL CONTENTS

[0006] Therefore, the utility model provides a microfluidic chip.

[0007] The microfluidic chip provided by the utility model comprises a chip main body, and the chip main body is provided with:

[0008] a first sample inlet;

[0009] a second sample inlet;

[0010] a third sample inlet;

[0011] a first detection system in communication with the first sample inlet, the first detection system configured to optically detect a sample input from the first sample inlet;

[0012] a second detection system in communication with the second sample inlet, the second detection system configured to optically detect and impedance detect a sample input from the second sample inlet;

[0013] a third detection system in communication with the third sample inlet, the third detection system configured to impedance detect a sample input from the third sample inlet;

[0014] a first drive channel, a first end of the first drive channel in communication with the first detection system, a second end of the first drive channel configured to connect a first drive, the first drive configured to drive a liquid to move within the first detection system through the first drive channel;

[0015] a second drive channel, a first end of the second drive channel in communication with the second detection system, a second end of the second drive channel configured to connect a second drive, the second drive configured to drive a liquid to move within the second detection system through the second drive channel;

[0016] a third drive channel, a first end of the third drive channel in communication with the third detection system, a second end of the third drive channel configured to connect a third drive, the third drive configured to drive a liquid to move within the third detection system through the third drive channel.

[0017] From the above technical scheme can be seen, the microfluidic chip provided by the utility model, first, by integrating the design of the first detection system, the second detection system and the third detection system on the small volume microfluidic chip, compared with the existing sample detection instrument, the microfluidic chip is more integrated, miniaturized, more portable, and the cost is lower, the operator can use the microfluidic chip for sample detection in any suitable environment, and too many use restrictions will not be caused. Secondly, the microfluidic chip is disposable consumables, which can be directly discarded after sample detection, and is more convenient to use. The waste liquid after sample detection remains in the microfluidic chip, and there is no problem of pollution caused by secondary flow of waste liquid, and the operator does not need to perform additional processing, even if an adverse factor affects the sample detection process, a microfluidic chip can be directly replaced for detection again, thereby reducing the influence on the detection efficiency. Furthermore, the microfluidic chip is used for sample detection, can accurately and quantitatively realize the acquisition and processing of the sample, and the required sample amount and reagent amount are relatively small, thereby reducing the waste of the sample and the reagent. In addition, the first detection system, the second detection system and the third detection system are independent of each other, the sample detection performed among the three is independent of each other and does not interfere with each other, and the operator can understand different functions realized by the three and make corresponding operations. For example, each detection system can be driven and controlled in a targeted manner, for example, if the sample mixing effect in a certain detection system is not good or the flow speed is slow, the driving channel corresponding to the detection system can be driven alone to make the sample mixing effect or the flow speed reach the effect required by the operator. It should be noted that the first driving channel, the second driving channel and the third driving channel are used for driving the liquid flowing in the first detection system, the second detection system and the third detection system respectively, so that the sample can flow normally in the first detection system, the second detection system and the third detection system to perform related processing. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the drawings needed to be used in the embodiment description will be briefly introduced, and obviously, the drawings in the following description are some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained without creative labor.

[0019] Figure 1 It is the structure schematic diagram of the microfluidic chip of an embodiment of the utility model;

[0020] Figure 2 It is the explosion schematic diagram of the microfluidic chip of an embodiment of the utility model;

[0021] Figure 3 It is the explosion schematic diagram of the top view of the microfluidic chip of an embodiment of the utility model;

[0022] Figure 4 This is an exploded view of the bottom of a microfluidic chip according to an embodiment of the present invention;

[0023] Figure 5 This is a schematic diagram of the substrate from the front view according to an embodiment of the present invention;

[0024] Figure 6 This is a schematic diagram of the substrate from the back view according to an embodiment of the present invention;

[0025] Figure 7 This is a schematic diagram of the structure of the cover plate facing away from the substrate according to an embodiment of the present invention;

[0026] Figure 8 This is a schematic diagram of the structure of the cover plate facing the substrate according to an embodiment of the present invention;

[0027] Figure 9 This is an exploded view of a partial structure of a microfluidic chip proposed in an embodiment of this utility model;

[0028] Figure 10 This is an exploded view of a partial structure of a microfluidic chip proposed in an embodiment of this utility model;

[0029] Figure 11 yes Figure 6 A magnified view of a portion of point G in the middle.

[0030] Furthermore, because the channels of the proposed microfluidic chip are partially located on the front side of the substrate, partially on the back side of the substrate, and partially on the cover plate, therefore... Figure 5 , Figure 6 , Figure 7 and Figure 8 The letters ABCDEF are used to mark the passageways, aiming to clearly indicate their direction. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are all within the protection scope of the present utility model.

[0032] like Figures 1 to 6As shown, the embodiment of the utility model discloses a kind of microfluidic chip, and microfluidic chip includes chip main body 100, and chip main body 100 is provided with first sample inlet 10, second sample inlet 20, third sample inlet 30, first detection system 40, second detection system 50, third detection system 60, first drive channel 70, second drive channel 80 and third drive channel 90.

[0033] First detection system 40 is communicated with first sample inlet 10, and first detection system 40 is used for carrying out optical detection to the sample input from first sample inlet 10.Second detection system 50 is communicated with second sample inlet 20, and second detection system 50 is used for carrying out optical detection and impedance detection to the sample input from second sample inlet 20.Second detection system 50 is communicated with third sample inlet 30, and third detection system 60 is used for carrying out impedance detection to the sample input from third sample inlet 30.

[0034] The first end of first drive channel 70 is communicated with first detection system 40, and the second end D1 of first drive channel 70 is used for connecting first driver, and first driver drives liquid to run in the inside of first detection system 40 by first drive channel 70.The first end of second drive channel 80 is communicated with second detection system 50, and the second end E1 of second drive channel 80 is used for connecting second driver, and second driver drives liquid to run in the inside of second detection system 50 by second drive channel 80.The first end of third drive channel 90 is communicated with third detection system 60, and the second end F1 of third drive channel 90 is used for connecting third driver, and third driver drives liquid to run in the inside of third detection system 60 by third drive channel 90.

[0035] The microfluidic chip provided by the embodiment of the utility model, firstly, the first detection system 40, the second detection system 50 and the third detection system 60 are integrated on the microfluidic chip with small volume, compared with the existing sample detection instrument, the microfluidic chip is more integrated, miniaturized, more portable and lower in cost, and the operator can use the microfluidic chip to detect samples in any suitable environment without too many use restrictions.Secondly, the microfluidic chip is disposable consumables, which can be directly discarded after sample detection, and is more convenient to use. After sample detection, the waste liquid remains in the microfluidic chip, and there is no problem of pollution caused by secondary outflow of waste liquid, and the operator does not need to perform additional processing. Even if there is an adverse factor during sample detection, a microfluidic chip can be directly replaced for detection again, thereby reducing the influence on detection efficiency. Furthermore, the microfluidic chip is used for sample detection, can accurately and quantitatively realize acquisition and processing of samples, and the required sample amount and reagent amount are relatively small, thereby reducing waste of samples and reagents. In addition, the first detection system 40, the second detection system 50 and the third detection system 60 are independent of each other, and sample detection performed among the three is independent and does not interfere with each other, so that the operator can understand different functions realized by the three and make corresponding operations. For example, each detection system can be driven and controlled in a targeted manner. If the sample mixing effect in a certain detection system is not good or the flow speed is slow, the driving channel corresponding to the detection system can be driven separately to make the sample mixing effect or flow speed reach the effect required by the operator. It should be noted that the first driving channel 70, the second driving channel 80 and the third driving channel 90 are used to drive the liquid flowing in the first detection system 40, the second detection system 50 and the third detection system 60 respectively, so that the sample can flow normally in the first detection system 40, the second detection system 50 and the third detection system 60 to perform related processing.

[0036] In some embodiments, the components of the microfluidic chip are made by one-piece precision injection molding, which can improve the integrity of the microfluidic chip and ensure its accuracy during sampling, reagent addition and dilution.

[0037] In some embodiments, the first driver, the second driver and the third driver are three independent injectors, which are simple in structure and easy to control.

[0038] In some embodiments, the samples input from the first inlet 10, the second inlet 20, and the third inlet 30 are all blood samples. The first detection system 40 is used to detect the blood sample to obtain its immunoturbidimetric index, the second detection system 50 is used to detect the blood sample to obtain its hemoglobin and white blood cell differential indices, and the third detection system 60 is used to detect the blood sample to obtain its red blood cell and platelet indices. In this embodiment, the proposed microfluidic chip can simultaneously and independently detect the circulating blood sample to obtain immunoturbidimetric, hemoglobin, white blood cell differential, red blood cell, and platelet indices, thereby achieving routine blood count testing. Of course, the first detection system 40, the second detection system 50, and the third detection system 60 can also be used to detect other indicators in the blood sample, depending on the actual design requirements. It should also be noted that the proposed microfluidic chip is not limited to the detection of blood samples; it can also be used to detect other samples, depending on the actual design requirements.

[0039] The "immunoturbidimetric indicators" refer to indicators measured using immunoturbidimetry, such as uric acid concentration, C-reactive protein concentration, and serum amyloid protein concentration. The "hemoglobin indicators" include hemoglobin concentration. The "white blood cell differential indicators" include white blood cell classification and quantity; for example, white blood cells include neutrophils, lymphocytes, monocytes, eosinophils, and basophils, and the white blood cell differential indicators include the quantity of these five cell types. The "red blood cell indicators" include the number of red blood cells. The "platelet indicators" include the number of platelets.

[0040] like Figure 1 and Figure 2 As shown, in some embodiments, the first injection port 10, the second injection port 20, and the third injection port 30 are disposed on the same side of the chip body 100 and arranged adjacent to each other. The microfluidic chip also includes a sealing member 200, which is closable and connected to the chip body 100. The sealing member 200 is used to block the first injection port 10, the second injection port 20, and the third injection port 30. In this embodiment, by disposing the first injection port 10, the second injection port 20, and the third injection port 30 on the same side of the chip body 100 and arranging them adjacent to each other, sampling by the microfluidic chip is facilitated. Taking the use of microfluidic chips for blood sample detection as an example, in one specific embodiment, the examinee or medical personnel use a lancet to prick the examinee's sampling site. When a blood droplet appears at the sampling site, the first sampling port 10, the second sampling port 20, and the third sampling port 30 are brought into contact with the blood droplet. The blood droplet enters the first detection system 40, the second detection system 50, and the third detection system 60 through the corresponding sampling ports, making sampling extremely convenient.

[0041] Further, by setting the blocking member 200 to block the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30, a closed environment is formed at the opening end of the first detection system 40, the second detection system 50 and the third detection system 60, and neither gas nor liquid can pass through. When the gas is filled through the first driving channel 70, the second driving channel 80 and the third driving channel 90, the gas can only flow along the first detection system 40, the second detection system 50 and the third detection system 60 due to the blocking of the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30 by the blocking member 200, facilitating the subsequent steps.

[0042] In some embodiments, the blocking member 200 is rotationally arranged on the chip body 100. The first sample inlet 10, the second sample inlet 20 and the third sample inlet 30 can be closed by rotating the blocking member 200 to contact the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30, and the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30 can be opened by rotating the blocking member 200 to separate from the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30, facilitating operation and avoiding the situation that the blocking member 200 is separated from the substrate and lost. Of course, in other embodiments, the blocking member 200 can be arranged to be detachable relative to the chip body 100, which can be determined according to actual design needs.

[0043] In some embodiments, the blocking member 200 can be made of silica gel to reduce friction on the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30. Since silica gel has a certain deformability, it can better adapt to the first sample inlet 10, the second sample inlet 20 and the third sample inlet 30, improving the sealing effect.

[0044] As shown in FIGS. 1, 2 and 3, in some embodiments, the first driving channel 70, the second driving channel 80 and the third driving channel 90 are arranged on the chip body 100. Figure 5 As shown in FIGS. 1, 2 and 3, in some embodiments, the first driving channel 70, the second driving channel 80 and the third driving channel 90 are arranged on the chip body 100. Figure 6 As shown in FIGS. 1, 2 and 3, in some embodiments, the second end D1 of the first driving channel 70, the second end E1 of the second driving channel 80 and the second end F1 of the third driving channel 90 are arranged on the same side of the chip body 100 and adjacent to each other. In this embodiment, the first driver, the second driver and the third driver can be arranged correspondingly adjacent to each other, facilitating the arrangement of the first driver, the second driver and the third driver.

[0045] In some embodiments, the chip body 100 includes a substrate 100a, the surface of the substrate 100a is shaped into a groove, and the first driving channel 70, the second driving channel 80 and the third driving channel 90 are formed by covering a film or a cover plate on the surface of the substrate 100a. The mixing channel described below can also be formed in this way, which will not be described in detail.

[0046] As shown in FIGS. 1, 2 and 3, in some embodiments, the first driving channel 70, the second driving channel 80 and the third driving channel 90 are arranged on the chip body 100. Figures 2 to 6As shown, in some embodiments, the first detection system 40 includes a first buffer pool 41, a second buffer pool 42, a first mixing channel 43, a first reagent adding part 44, a first optical detection part 45, and a second mixing channel 46. The first mixing channel 43 is connected to the first sample inlet 10 and the first buffer pool 41. The first reagent adding part 44 is connected to the first sample inlet 10, and is configured to provide a first reagent mixed with the sample. The first optical detection part 45 is connected to the first reagent adding part 44, and is configured to detect the mixture of the sample and the first reagent. The second mixing channel 46 is connected to the first optical detection part 45 and the second buffer pool 42. The first end of the first driving channel 70 is connected to one of the first buffer pool 41 and the second buffer pool 42, and the first driver drives the sample and the first reagent to flow back and forth in the first mixing channel 43 and the second mixing channel 46 by supplying air or suction to the first driving channel 70.

[0047] After the first reagent adding part 44 provides the first reagent mixed with the sample, the mixture of the sample and the first reagent may not be uniform enough, which affects the accuracy of subsequent optical detection. In this embodiment, the first mixing channel 43 connected to the first sample inlet 10 and the second mixing channel 46 connected to the first optical detection part 45 are provided, and before optical detection, the first driver drives the sample and the first reagent to flow back and forth in the first mixing channel 43 and the second mixing channel 46 by supplying air or suction to the first driving channel 70, so as to achieve the purpose of mixing the sample and the first reagent.

[0048] In actual specific detection process, when the first driver drives the sample and the first reagent to mix to a certain degree by supplying air or suction to the first driving channel 70 for a certain number of times, the first driver drives the mixed sample liquid into the first optical detection part 45 for optical detection by supplying air to the first driving channel 70.

[0049] In some embodiments, the first optical detection part 45 has a first optical detection window, and the mixed sample liquid after mixing can stay in the first optical detection window. The upper surface and the lower surface of the first optical detection window are respectively provided with an upper light-transmitting film and a lower light-transmitting film. An upper side of the upper light-transmitting film is provided with a light source, such as an LED light source with a wavelength of 567 nm. A lower side of the lower light-transmitting film is provided with a light receiver. The light source and the light receiver are connected with a processor.

[0050] For example, for the CRP (C-reactive protein) detection of blood cells, when the detection is performed, the light source is turned on, the light penetrates the upper light-transmitting film and passes through the mixed sample liquid in the first optical detection window, the blood cells in the mixed sample liquid absorb the light under the chemical reaction, the light is received by the light receiver after being absorbed by the blood cells to a certain extent through the lower light-transmitting film, the controller detects the change curve of the incident light intensity and the transmitted light intensity, and calculates the absorbance of the blood cells in the mixed sample liquid, so as to further calculate the CRP index of the blood cells in the mixed sample liquid. In other embodiments, the first detection system 40 can also be used to detect the SAA (serum amyloid A) index of the blood cells.

[0051] In some embodiments, after the optical detection is completed, the first driver drives the mixed sample liquid into the first buffer pool 41 or the second buffer pool 42 by inflating the first driving channel 70, so as to avoid the pollution caused by the waste liquid flowing out.

[0052] As shown in FIG. 1, Figure 5 In some embodiments, the chip body 100 is further provided with a first sample quantification part 101, a first sample conveying microchannel 102 and a second sample conveying microchannel 103. The first sample quantification part 101 is in communication with the first sample inlet 10, and is used for quantifying the sample input into the first detection system 40 from the first sample inlet 10. One end of the first sample conveying microchannel 102 is in communication with the first sample quantification part 101, and the other end of the first sample conveying microchannel 102 is in communication with the first mixing channel 43. One end of the second sample conveying microchannel 103 is in communication with the first sample quantification part 101, and the other end of the second sample conveying microchannel 103 is in communication with the first reagent adding part 44. Optionally, the first sample conveying microchannel 102 and the second sample conveying microchannel 103 are in a capillary structure. In this embodiment, by providing the first sample quantification part 101, the sample amount entering the first detection system 40 can be controlled to a certain volume, and by controlling the amount of the reagent input by the first reagent adding part 44, the sample entering the first detection system 40 can be diluted to a specified concentration, so as to improve the detection effect. In some embodiments, the volume of the first sample quantification part 101 is 0.8 μL.

[0053] As shown in FIG. 1, Figure 3 , Figure 9 and Figure 10As shown in the drawings, in some embodiments, the first reagent adding part 44 comprises a first accommodating part 441, a first reagent pool 442 and a first reagent container 443. The first sample inlet 10 and the first optical detection part 45 are in communication with the first reagent pool 442. The first reagent container 443 is accommodated in the first accommodating part 441, the first reagent container 443 stores the first reagent, and the first reagent container 443 injects the first reagent into the first reagent pool 442 when pressed. Wherein, the first reagent pool 442 is arranged within the profile range of the first accommodating part 441. With this embodiment, by arranging the first reagent pool 442 within the profile range of the first accommodating part 441, that is, the first reagent pool 442 and the first accommodating part 441 share the structure, the space utilization can be improved to reduce the volume of the microfluidic chip.

[0054] As shown in the drawings, Figure 9 and Figure 10 As shown in the drawings, in some embodiments, the first accommodating part 441 comprises a first sink part 4411 and a first through-hole part 4412, the first through-hole part 4412 is arranged on the groove bottom surface of the first sink part 4411 and penetrates through the chip body 100, and the first reagent pool 442 is arranged on the groove bottom surface of the first sink part 4411 and is arranged in a spaced manner with the first through-hole part 4412. The first reagent container 443 comprises a first cup body 4431 and a first cup rim 4432, the first cup rim 4432 is arranged around the opening end of the first cup body 4431, the first cup rim 4432 is embedded in the first sink part 4411, the first cup rim 4432 is provided with a first reagent delivery microchannel in communication with the first reagent pool 442, and the first cup body 4431 is embedded in the first through-hole part 4412 and in communication with the first reagent delivery microchannel.

[0055] Wherein, the "first reagent delivery microchannel" is such that when the first reagent container 443 is not pressed, the first reagent in the first reagent container 443 cannot enter the first reagent pool 442 through the first reagent delivery microchannel, and only when the first reagent container 443 is pressed, the first reagent in the first reagent container 443 enters the first reagent pool 442 through the first reagent delivery microchannel.

[0056] As shown in the drawings, Figure 3 and Figure 4 As shown in the drawings, in some embodiments, the chip body 100 comprises a substrate 100a, the substrate 100a comprises a front surface and a back surface, one of the first mixing channel 43 and the second mixing channel 46 is arranged on the front surface of the substrate 100a, and the other of the first mixing channel 43 and the second mixing channel 46 is arranged on the back surface of the substrate 100a. With this embodiment, by arranging one of the first mixing channel 43 and the second mixing channel 46 on the front surface of the substrate 100a and the other of the first mixing channel 43 and the second mixing channel 46 on the back surface of the substrate 100a, the space utilization of the substrate 100a is improved, and the integration and miniaturization of the microfluidic chip are realized.

[0057] In some embodiments, the first mixing channel 43 and the second mixing channel 46 are arranged opposite to each other in the thickness direction of the substrate 100a. With this arrangement, the space utilization of the substrate 100a can be improved, and the microfluidic chip can be integrated and miniaturized.

[0058] As shown in FIG. 1, the first mixing channel 43 and the second mixing channel 46 are arranged opposite to each other in the thickness direction of the substrate 100a. With this arrangement, the space utilization of the substrate 100a can be improved, and the microfluidic chip can be integrated and miniaturized. Figures 2 to 6 、 Figure 11 As shown in FIG. 1, the first mixing channel 43 and the second mixing channel 46 are arranged opposite to each other in the thickness direction of the substrate 100a. With this arrangement, the space utilization of the substrate 100a can be improved, and the microfluidic chip can be integrated and miniaturized.

[0059] As shown in FIG. 1, the first mixing channel 43 and the second mixing channel 46 are arranged opposite to each other in the thickness direction of the substrate 100a. With this arrangement, the space utilization of the substrate 100a can be improved, and the microfluidic chip can be integrated and miniaturized.

[0060] Taking the second detection system 50 for detecting the blood sample to obtain the hemoglobin index and the white blood cell classification index of the blood sample as an example, the second reagent is a mixed reagent of a hemolytic agent and a hemoglobin reagent, which can dissolve red blood cells and leave white blood cells. When the second driver mixes the sample and the second reagent to a certain degree by inflating or sucking a certain number of times to the second driving channel 80, the second driver drives the mixed sample liquid into the second optical detection part 54 for optical detection by the second driving channel 80, and obtains the hemoglobin index of the blood sample.

[0061] In some embodiments, the second optical detection part 54 has a second optical detection window, and the mixed sample liquid after mixing can stay in the second optical detection window. The upper surface and the lower surface of the second optical detection window are respectively provided with an upper light transmission film and a lower light transmission film. An upper light source such as a 567nm LED light source is arranged above the upper light transmission film, and a light receiver is arranged below the lower light transmission film. The light source and the light receiver are connected with a processor.

[0062] When the hemoglobin index of blood cells is detected, the light source is turned on. The light penetrates the upper light transmission film and passes through the mixed sample liquid in the second optical detection window. The dissolved blood cells in the mixed sample liquid will absorb light under chemical reaction. After the light is absorbed by the dissolved blood cells to a certain extent, it is received by the light receiver through the lower light transmission film. The controller detects the change curve of the incident light intensity and the transmitted light intensity, calculates the absorbance of the dissolved blood cells in the mixed sample liquid, and further calculates the hemoglobin index of the blood cells in the mixed sample liquid.

[0063] After the optical detection is completed, the second driver drives the mixed sample liquid into the first sheath flow part 51 by the second driving channel 80. The remaining white blood cells in the mixed sample liquid pass through the first sheath flow part 51 for subsequent impedance detection to obtain the white blood cell classification index of the flowing blood.

[0064] For example, the first detection system 40 is used to detect the blood sample to obtain the hemoglobin index and the white blood cell classification index of the blood sample. The first reagent is a mixed reagent of a hemolytic agent and a hemoglobin reagent, which can dissolve red blood cells and leave white blood cells. When the first driver mixes the sample and the first reagent to a certain degree by inflating or sucking a certain number of times to the first driving channel 70, the first driver drives the mixed sample liquid into the first optical detection part 44 for optical detection by the first driving channel 70, and obtains the hemoglobin index of the blood sample. Figure 5As shown in some embodiments, the chip body 100 is further provided with a second sample quantifying part 104, a third sample conveying microchannel 105 and a fourth sample conveying microchannel 106. The second sample quantifying part 104 is in communication with the second sample inlet 20, and the second sample quantifying part 104 is used for quantifying the sample input from the second sample inlet 20 into the second detection system 50. One end of the third sample conveying microchannel 105 is in communication with the second sample quantifying part 104, and the other end of the third sample conveying microchannel 105 is in communication with the second reagent adding part 53. One end of the fourth sample conveying microchannel 106 is in communication with the second sample quantifying part 104, and the other end of the fourth sample conveying microchannel 106 is in communication with the third buffer pool 52. Optionally, the third sample conveying microchannel 105 and the fourth sample conveying microchannel 106 are of capillary structure. With this embodiment, by providing the second sample quantifying part 104, the amount of sample entering the second detection system 50 can be controlled to a certain volume, and by controlling the amount of reagent input by the second reagent adding part 53, the sample entering the second detection system 50 can be diluted to a specified concentration, so as to improve the detection effect. In some embodiments, the volume of the second sample quantifying part 104 is 0.8 μL.

[0065] As shown in some embodiments, Figure 3 , Figure 9 and Figure 10 , the second reagent adding part 53 comprises a second containing part 531, a second reagent pool 532 and a second reagent container 533. The second sample inlet 20 and the second optical detection part 54 are in communication with the second reagent pool 532. The second reagent container 533 is contained in the second containing part 531, the second reagent container 533 stores the second reagent therein, and the second reagent container 533 injects the second reagent into the second reagent pool 532 when pressed. Wherein, the second reagent pool 532 is arranged within the profile range of the second containing part 531. With this embodiment, by arranging the second reagent pool 532 within the profile range of the second containing part 531, i.e. the second reagent pool 532 and the second containing part 531 share the structure, the space utilization can be improved, so as to reduce the volume of the microfluidic chip.

[0066] As shown in some embodiments, Figure 9 and Figure 10As shown, in some embodiments, the second receiving portion 531 includes a second sink portion 5311 and a second through-hole portion 5312. The second through-hole portion 5312 is disposed on the bottom surface of the sink portion 5311 and penetrates the chip body 100. The second reagent pool 532 is disposed on the bottom surface of the sink portion 5311 and spaced apart from the second through-hole portion 5312. The second reagent container 533 includes a second cup body 5331 and a second cup rim 5332. The second cup rim 5332 surrounds the opening end of the second cup body 5331 and is embedded in the second sink portion 5311. The second cup rim 5332 is provided with a second reagent delivery microchannel communicating with the second reagent pool 532. The second cup body 5331 is embedded in the second through-hole portion 5312 and communicates with the second reagent delivery microchannel.

[0067] Specifically, when the second reagent container 533 is not pressed, the second reagent in the second reagent container 533 cannot enter the second reagent pool 532 through the second reagent delivery microchannel. Only when the second reagent container 533 is pressed can the second reagent in the second reagent container 533 enter the second reagent pool 532 through the second reagent delivery microchannel.

[0068] like Figure 5 and Figure 6 As shown, in some embodiments, the chip body 100 includes a substrate 100a, which has a front side and a back side. A third mixing channel 55 is partially disposed on the front side of the substrate 100a and partially disposed on the back side of the substrate 100a. In this embodiment, by partially disposing the third mixing channel 55 on the front side of the substrate 100a and partially disposing it on the back side of the substrate 100a, the space utilization of the substrate 100a is improved, achieving the integration and miniaturization of the microfluidic chip.

[0069] In some embodiments, the third mixing channel 55 located on the front side of the substrate 100a and the third mixing channel 55 located on the back side of the substrate 100a are disposed opposite to each other in the thickness direction of the substrate 100a. In this embodiment, the space utilization of the substrate 100a can be improved, and the integration and miniaturization of the microfluidic chip can be realized.

[0070] like Figures 4 to 6 , Figure 11As shown in some embodiments, the first sheath flow part 51 includes a first gem hole 511, a first laminar flow groove 512, and a first auxiliary flow groove 513. The first laminar flow groove 512 and the first auxiliary flow groove 513 are respectively located on both sides of the first gem hole 511, and the first laminar flow groove 512, the first gem hole 511, and the first auxiliary flow groove 513 form an hourglass structure. The first sample liquid inlet 51a and the first sheath liquid inlet 51b communicate with the first laminar flow groove 512, and the first auxiliary flow inlet 51c, the second auxiliary flow inlet 51d, and the first waste liquid outlet 51e communicate with the first auxiliary flow groove 513.

[0071] As shown in some embodiments, Figures 4 to 6 , Figure 11 As shown in some embodiments, the first sheath flow part 51 further includes a first flow guide part 514, a first branch flow channel 515, and a second branch flow channel 516. The first flow guide part 514 is arranged in the middle of the first laminar flow groove 512, and the first flow guide part 514 is provided with a first flow guide groove 5141, one end of the first flow guide groove 5141 communicates with the first sample liquid inlet 51a, and the other end of the first flow guide groove 5141 extends towards the first gem hole 511. The first sheath liquid inlet 51b is arranged on the side of the first sample liquid inlet 51a opposite to the first gem hole 511, one end of the first branch flow channel 515 and one end of the second branch flow channel 516 communicate with the first sample liquid inlet 51a, the other end of the first branch flow channel 515 and the other end of the second branch flow channel 516 communicate with the first laminar flow groove 512, and the first flow guide part 514 is located between the first branch flow channel 515 and the second branch flow channel 516.

[0072] As shown in some embodiments, Figures 4 to 6 , Figure 11 As shown in some embodiments, the first sheath flow part 51 further includes a third branch flow channel 517 and a fourth branch flow channel 518, one end of the third branch flow channel 517 communicates with the first auxiliary flow inlet 51c, the other end of the third branch flow channel 517 communicates with the first auxiliary flow groove 513, one end of the fourth branch flow channel 518 communicates with the second auxiliary flow inlet 51d, and the other end of the fourth branch flow channel 518 communicates with the first auxiliary flow groove 513, and the first gem hole 511 is located between the third branch flow channel 517 and the fourth branch flow channel 518.

[0073] In some embodiments, the microfluidic chip further includes a first electrode and a second electrode, the first electrode is arranged in the first laminar flow groove 512, and the second electrode is arranged in the first auxiliary flow groove 513.

[0074] Taking the impedance detection of blood cells by the first sheath flow part 51 using the sheath flow technology to determine the white blood cell classification index of the blood cells as an example, the detection process is as follows:

[0075] The third mixing channel 55 receives the mixed sample liquid with only white blood cells after the red blood cells are lysed, and the mixed sample liquid enters the first flow channel 5141 from the first sample liquid inlet 51a. At the same time, the first sheath liquid adding part 56 injects the first sheath liquid into the first sheath liquid channel 57 and the second sheath liquid channel 58 after being extruded. The first sheath liquid in the first sheath liquid channel 57 enters the first sheath flow part 51 from the first sheath liquid inlet 51b, and then enters the first branch flow channel 515 and the second branch flow channel 516, respectively. The sheath liquid in the first branch flow channel 515 and the second branch flow channel 516 flows to the first gem hole 511 together with the white blood cells coming out of the first flow channel 5141. According to the impedance counting principle, when the white blood cells pass through the first gem hole 511, the conductivity between the first electrode and the second electrode changes, that is, the resistance value changes, so that the counting can be realized by counting the changed peak value through the impedance detection electrode. The white blood cell classification index of the blood flowing through the second detection system 50 is detected. The first sheath liquid flowing in the first branch flow channel 515 and the sheath liquid flowing in the second branch flow channel 516 can be used as the source of laminar flow of the sheath flow technology to ensure the normal operation of the sheath flow detection. At the same time when the first sheath liquid adding part 56 is extruded, the first sheath liquid adding part 56 injects the first sheath liquid into the second sheath liquid channel 58. The first sheath liquid in the second sheath liquid channel 58 enters the first sheath flow part 51 from the first auxiliary flow inlet 51c and the second auxiliary flow inlet 51d. The first sheath liquid entering from the first auxiliary flow inlet 51c enters the third branch flow channel 517, and the first sheath liquid entering from the second auxiliary flow inlet 51d enters the fourth branch flow channel 518. The two parts of the first sheath liquid enter the first auxiliary flow channel 513, which can keep the flow field in the first auxiliary flow channel 513 stable and avoid turbulence of the mixed liquid, so as to affect the flow of the mixed liquid from the first laminar flow channel 512 to the first auxiliary flow channel 513. After the impedance detection is completed, the second driver inputs gas to the second detection system 50 through the second driving channel 80 to drive the waste liquid in the first sheath flow part 51 to the first waste liquid pool 59 for centralized treatment, so as to avoid pollution caused by the waste liquid flowing out.

[0076] In some embodiments, the pore diameter of the first gem hole 511 is 0.02 mm to 1 mm. In this implementation, the size of the first gem hole 511 is controlled within the above range, which controls the difficulty of the manufacturing process and also matches the cell volume of the white blood cells, meets the demand for detecting the white blood cells, and makes the white blood cells shot through the first gem hole 511 keep relatively ordered silicon, so as to ensure the accuracy of blood cell detection. Preferably, the pore diameter of the first gem hole 511 is 0.1 mm.

[0077] As Figure 5 and Figure 6As shown in some embodiments, the chip body 100 includes a substrate 100a, the substrate 100a includes a front surface and a back surface, the first sheath liquid channel 57 and the second sheath liquid channel 58 are arranged on the front surface of the substrate 100a, and the first sheath flow part 51 is arranged on the back surface of the substrate 100a. In this embodiment, by arranging the first sheath liquid channel 57 and the second sheath liquid channel 58 on the front surface of the substrate 100a and arranging the first sheath flow part 51 on the back surface of the substrate 100a, the space of the substrate 100a is utilized as much as possible, and the integration and miniaturization of the microfluidic chip are achieved.

[0078] As shown in some embodiments, Figure 3 , Figure 9 and Figure 10 , in some embodiments, the first sheath liquid adding part 56 includes a first accommodating part 561, a first sheath liquid pool 562, a second sheath liquid pool 563, and a first sheath liquid container 564. The first sheath liquid channel 57 communicates with the first sheath liquid pool 562. The second sheath liquid channel 58 communicates with the second sheath liquid pool 563. The first sheath liquid container 564 is accommodated in the first accommodating part 561, the first sheath liquid container 564 stores the first sheath liquid, and the first sheath liquid container 564 injects the first sheath liquid into the first sheath liquid pool 562 and the second sheath liquid pool 563 when pressed. Among them, the first sheath liquid pool 562 and the second sheath liquid pool 563 are arranged within the contour range of the first accommodating part 561. In this embodiment, by arranging the first sheath liquid pool 562 and the second sheath liquid pool 563 within the contour range of the first accommodating part 561, that is, the first sheath liquid pool 562 and the second sheath liquid pool 563 share the structure with the first accommodating part 561, the space can be reasonably utilized, and the integration and miniaturization of the microfluidic chip are achieved.

[0079] As shown in some embodiments, Figure 9 and Figure 10 , in some embodiments, the first accommodating part 561 includes a first groove part 5611, a first accommodating hole part 5612, and a second accommodating hole part 5613, the first accommodating hole part 5612 and the second accommodating hole part 5613 are arranged on the groove bottom surface of the first groove part 5611 and penetrate through the chip body 100, and the first sheath liquid pool 562 and the second sheath liquid pool 563 are arranged on the groove bottom surface of the first groove part 5611. The first sheath liquid container 564 includes a first cavity 5641, a second cavity 5642, and a first cavity side 5643, the first cavity side 5643 surrounds the four sides of the opening end of the first cavity 5641 and the second cavity 5642, the first cavity side 5643 is embedded in the first groove part 5611, the first cavity side 5643 is provided with a first sheath liquid delivery microchannel communicating with the first sheath liquid pool 562 and a second sheath liquid delivery microchannel communicating with the second sheath liquid pool 563, the first cavity 5641 is embedded in the first accommodating hole part 5612 and communicates with the first sheath liquid delivery microchannel, and the second cavity 5642 is embedded in the second accommodating hole part 5613 and communicates with the second sheath liquid delivery microchannel.

[0080] Specifically, when the first sheath fluid container 564 is not pressed, the first sheath fluid in the first sheath fluid container 564 cannot enter the first sheath fluid pool 562 through the first sheath fluid delivery microchannel, and the first sheath fluid in the first sheath fluid container 564 cannot enter the second sheath fluid pool 563 through the second sheath fluid delivery microchannel. Only when the first sheath fluid container 564 is pressed can the first sheath fluid in the first sheath fluid container 564 enter the first sheath fluid pool 562 through the first sheath fluid delivery microchannel, and the first sheath fluid in the first sheath fluid container 564 enter the second sheath fluid pool 563 through the second sheath fluid delivery microchannel.

[0081] like Figures 2 to 8 , Figure 11 As shown, in some embodiments, the third detection system 60 includes a second sheath flow section 61, a third reagent addition section 62, a fourth mixing channel 63, a fifth mixing channel 64, a second sheath fluid addition section 65, a third sheath fluid channel 66, a fourth sheath fluid channel 67, and a second waste liquid pool 68. The second sheath flow section 61 includes a second sample liquid inlet 61a, a second sheath fluid inlet 61b, a third auxiliary flow inlet 61c, a fourth auxiliary flow inlet 61d, and a second waste liquid outlet 61e. The third reagent addition section 62 is connected to the third sample inlet 30 and is used to provide a third reagent mixed with the sample. The fourth mixing channel 63 is connected to the third reagent addition section 62. The fifth mixing channel 64 is connected to the third sample inlet 30 and the second sample liquid inlet 61a. The second sheath fluid addition section 65 is used to provide sheath fluid to the second sheath flow section 61. The third sheath fluid channel 66 is connected to the second sheath fluid addition section 65 and the second sheath fluid inlet 61b. One end of the fourth sheath fluid channel 67 is connected to the second sheath fluid addition section 65, and the other end of the fourth sheath fluid channel 67 is connected to the third auxiliary flow inlet 61c and the fourth auxiliary flow inlet 61d. The second waste liquid pool 68 is connected to the second waste liquid outlet 61e and is used to receive the waste liquid discharged from the second sheath flow section 61. The first end of the third drive channel 90 is connected to the fourth mixing channel 63. The third actuator drives the sample and reagent to reciprocate within the fourth mixing channel 63 and the fifth mixing channel 64 by inflating or deflating the third drive channel 90.

[0082] Since the mixing of the third reagent and the sample provided by the third reagent addition unit 62 may not be uniform enough, it will affect the accuracy of optical detection. In this embodiment, by setting a fourth mixing channel 63 connected to the third reagent addition unit 62 and a fifth mixing channel 64 connected to the third sample inlet 30, before optical detection, the third driver drives the sample and the third reagent to flow back and forth in the fourth mixing channel and the fifth mixing channel 64 by inflating or deflating the third driving channel 90, thereby achieving the purpose of mixing the sample and the third reagent.

[0083] For example, the third detection system 60 is used to detect the blood sample to obtain the red blood cell index and the platelet index of the blood sample. After the sample is mixed with the third reagent to obtain a mixed sample liquid, the mixed sample liquid flows through the fifth mixing channel 64 to the second sheath flow part 61 to perform impedance detection to obtain the red blood cell index and the platelet index.

[0084] As shown in Figure 5 In some embodiments, the chip body 100 is further provided with a third sample quantifying part 107, a fifth sample conveying microchannel 108 and a sixth sample conveying microchannel 109. The third sample quantifying part 107 is in communication with the third sample inlet 30, and the third sample quantifying part 107 is used to quantify the sample input into the third detection system 60 from the third sample inlet 30. One end of the fifth sample conveying microchannel 108 is in communication with the third sample quantifying part 107, and the other end of the fifth sample conveying microchannel 108 is in communication with the third reagent adding part 62. One end of the sixth sample conveying microchannel 109 is in communication with the third sample quantifying part 107, and the other end of the sixth sample conveying microchannel 109 is in communication with the fifth mixing channel 64. Optionally, the fifth sample conveying microchannel 108 and the sixth sample conveying microchannel 109 are capillary structures. In this embodiment, by providing the third sample quantifying part 107, the amount of the sample input into the third detection system 60 can be controlled to a certain volume, and by controlling the amount of the reagent input by the third reagent adding part 62, the sample input into the third detection system 60 can be diluted to a specified concentration, so that the detection effect can be improved. In some embodiments, the volume of the third sample quantifying part 107 is 0.5 μL.

[0085] As shown in Figure 3 , Figure 9 and Figure 10 In some embodiments, the third reagent adding part 62 includes a third containing part 621, a third reagent pool 622 and a third reagent container 623. The third reagent pool 622 is in communication with the third sample inlet 30. The third reagent container 623 is contained in the third containing part 621, the third reagent container 623 stores the third reagent, and the third reagent container 623 injects the third reagent into the third reagent pool 622 when pressed. The third reagent pool 622 is arranged within the profile range of the third containing part 621. In this embodiment, by arranging the third reagent pool 622 within the profile range of the third containing part 621, that is, the third reagent pool 622 and the third containing part 621 share the structure, the space utilization can be improved to reduce the volume of the microfluidic chip.

[0086] As shown in Figure 9 and Figure 10As shown, in some embodiments, the third receiving portion 621 includes a third sink portion 6211 and a third through-hole portion 6212. The third through-hole portion 6212 is disposed on the bottom surface of the third sink portion 6211 and penetrates the chip body 100. The third reagent pool 622 is disposed on the bottom surface of the third sink portion 6211 and spaced apart from the third through-hole portion 6212. The third reagent container 623 includes a third cup body 6231 and a third cup rim 6232. The third cup rim 6232 surrounds the opening end of the third cup body 6231 and is embedded in the third sink portion 6211. The third cup rim 6232 is provided with a third reagent delivery microchannel communicating with the third reagent pool 622. The third cup body 6231 is embedded in the third through-hole portion 6212 and communicates with the third reagent delivery microchannel.

[0087] Specifically, when the third reagent container 623 is not pressed, the third reagent in the third reagent container 623 cannot enter the third reagent pool 622 through the third reagent delivery microchannel. Only when the third reagent container 623 is pressed can the third reagent in the third reagent container 623 enter the third reagent pool 622 through the third reagent delivery microchannel.

[0088] like Figures 3 to 6 As shown, in some embodiments, the chip body 100 includes a substrate 100a, which has a front side and a back side. A fourth mixing channel 63 is partially disposed on the front side of the substrate 100a and partially disposed on the back side of the substrate 100a. In this embodiment, by partially disposing the fourth mixing channel 63 on the front side of the substrate 100a and partially disposing it on the back side of the substrate 100a, the space utilization of the substrate 100a is improved, achieving the integration and miniaturization of the microfluidic chip.

[0089] In some embodiments, the fourth mixing channel 63 located on the front side of the substrate 100a and the fourth mixing channel 63 located on the back side of the substrate 100a are disposed opposite to each other in the thickness direction of the substrate 100a. In this embodiment, the space utilization of the substrate 100a can be improved, and the integration and miniaturization of the microfluidic chip can be realized.

[0090] like Figure 3 , Figure 4 , Figure 7 and Figure 8As shown in some embodiments, the chip body 100 further comprises a cover plate 100b covering the front surface of the substrate 100a, and the fifth mixing channel 64 is arranged on the cover plate 100b. With this implementation, by arranging the fifth mixing channel 64 on the cover plate 100b, the space utilization of the chip body 100 is improved, and the integration and miniaturization of the microfluidic chip are realized. In addition, the cover plate 100b can prevent the microfluidic chip from being impacted by the outside world during transportation or before use, so as to prevent some structures or reagent containers from being damaged, thereby improving the safety of the microfluidic chip. At the same time, the cover plate 100b can also prevent the microfluidic chip from being affected by external water vapor, impurities, etc. before detection, thereby ensuring the accuracy of detection.

[0091] As shown in some embodiments, Figures 4 to 6 , Figure 11 As shown in some embodiments, the second sheath flow part 61 comprises a second gem hole 611, a second laminar flow groove 612, and a second auxiliary flow groove 613. The second laminar flow groove 612 and the second auxiliary flow groove 613 are respectively located on both sides of the second gem hole 611 and communicate with the second gem hole 611, and the second laminar flow groove 612, the second gem hole 611, and the second auxiliary flow groove 613 form an hourglass structure. The second sample liquid inlet 61a and the second sheath liquid inlet 61b communicate with the second laminar flow groove 612, and the third auxiliary flow inlet 61c, the fourth auxiliary flow inlet 61d, and the second waste liquid outlet 61e communicate with the second auxiliary flow groove 613.

[0092] As shown in some embodiments, Figures 4 to 6 , Figure 11 As shown in some embodiments, the second sheath flow part 61 further comprises a second flow guide part 614, a fifth branch flow channel 615, and a sixth branch flow channel 616. The second flow guide part 614 is arranged in the middle of the second laminar flow groove 612, and the second flow guide part 614 is provided with a second flow guide groove 6141, one end of the second flow guide groove 6141 communicates with the second sample liquid inlet 61a, and the other end of the second flow guide groove 6141 extends towards the second gem hole 611. The second sheath liquid inlet 61b is arranged on the side of the second sample liquid inlet 61a opposite to the second gem hole 611, one end of the fifth branch flow channel 615 and one end of the sixth branch flow channel 616 communicate with the second sample liquid inlet 61a, the other end of the fifth branch flow channel 615 and the other end of the sixth branch flow channel 616 communicate with the second laminar flow groove 612, and the second flow guide part 614 is located between the fifth branch flow channel 615 and the sixth branch flow channel 616.

[0093] As shown in some embodiments, Figures 4 to 6 , Figure 11As shown, in some embodiments, the second sheath flow part 61 further comprises a seventh branch flow channel 617 and an eighth branch flow channel 618, one end of the seventh branch flow channel 617 communicates with the third auxiliary flow inlet 61c, the other end of the seventh branch flow channel 617 communicates with the second auxiliary flow groove 613, one end of the eighth branch flow channel 618 communicates with the fourth auxiliary flow inlet 61d, the other end of the eighth branch flow channel 618 communicates with the second auxiliary flow groove 613, and the second gem hole 611 is located between the seventh branch flow channel 617 and the eighth branch flow channel 618.

[0094] In some embodiments, the microfluidic chip further comprises a third electrode and a fourth electrode, the third electrode is arranged on the second layer flow groove 612, and the fourth electrode is arranged on the second auxiliary flow groove 613.

[0095] Taking the red blood cell index and the platelet index of the blood cells as examples, the impedance detection of the blood cells is performed by the first sheath flow part 51 using the sheath flow technology, and the detection process is as follows:

[0096] The fifth mixing channel 64 receives the mixed sample liquid which enters the second flow channel 6141 from the second sample liquid inlet 61a, and the second sheath liquid adding part 65 injects the second sheath liquid into the third sheath liquid channel 66 and the fourth sheath liquid channel 67 after being extruded. The second sheath liquid in the third sheath liquid channel 66 enters the second sheath flow part 61 from the second sheath liquid inlet 61b, and then enters the fifth branch flow channel 615 and the sixth branch flow channel 616, respectively. The sheath liquid in the fifth branch flow channel 615 and the sixth branch flow channel 616 flows together with the red blood cells and platelets coming out of the second flow channel 6141 to the second gem hole 611. According to the impedance counting principle, when the red blood cells and platelets pass through the second gem hole 611, the conductivity between the third electrode and the fourth electrode changes, that is, the resistance value changes, so that the counting and size of the red blood cells and platelets in the blood flowing through the third detection system 60 can be counted by the impedance detection electrode.

[0097] In some embodiments, the pore diameter of the second gem hole 611 is 0.02mm to 1mm. In this implementation, the size of the second gem hole 611 is controlled within the above range, which controls the difficulty of the manufacturing process and also matches the cell volume of the red blood cells and platelets, meets the detection requirements of the red blood cells and platelets, and makes the red blood cells and platelets ejected through the second gem hole 611 maintain a relatively ordered crystal silicon, ensuring the accuracy of the blood cell detection. Preferably, the pore diameter of the second gem hole 611 is 0.07mm.

[0098] As Figure 5 and Figure 6As shown, in some embodiments, the chip body 100 includes a substrate 100a, which has a front side and a back side. A third sheath fluid channel 66 and a fourth sheath fluid channel 67 are disposed on the front side of the substrate 100a, and a second sheath flow portion 61 is disposed on the back side of the substrate 100a. In this embodiment, by disposing the third sheath fluid channel 66 and the fourth sheath fluid channel 67 on the front side of the substrate 100a and the second sheath flow portion 61 on the back side of the substrate 100a, the space of the substrate 100a is utilized as much as possible, thereby achieving the integration and miniaturization of the microfluidic chip.

[0099] like Figure 3 , Figure 9 and Figure 10 As shown, in some embodiments, the second sheath fluid adding unit 65 includes a second receiving portion 651, a third sheath fluid pool 652, a fourth sheath fluid pool 653, and a second sheath fluid container 654. A third sheath fluid channel 66 communicates with the third sheath fluid pool 652. A fourth sheath fluid channel 67 communicates with the fourth sheath fluid pool 653. The second sheath fluid container 654 is housed in the second receiving portion 651 and stores second sheath fluid. When the second sheath fluid container 654 is pressed, it injects the second sheath fluid into the third sheath fluid pool 652 and the fourth sheath fluid pool 653. The third sheath fluid pool 652 and the fourth sheath fluid pool 653 are located within the outline of the second receiving portion 651. In this embodiment, by setting the third sheath fluid pool 652 and the fourth sheath fluid pool 653 within the outline of the second accommodating portion 651, that is, by having the third sheath fluid pool 652 and the fourth sheath fluid pool 653 share a structure with the second accommodating portion 651, space can be rationally utilized to achieve the integration and miniaturization of the microfluidic chip.

[0100] like Figure 9 and Figure 10 As shown, in some embodiments, the second receiving portion 651 includes a second recess portion 6511, a third receiving hole portion 6512 and a fourth receiving hole portion 6513. The third receiving hole portion 6512 and the fourth receiving hole portion 6513 are disposed on the bottom surface of the second recess portion 6511 and penetrate the chip body 100. The third sheath fluid pool 652 and the fourth sheath fluid pool 653 are disposed on the bottom surface of the second recess portion 6511. The second sheath fluid container 654 includes a third cavity 6541, a fourth cavity 6542, and a second cavity edge 6543. The second cavity edge 6543 surrounds the opening ends of the third cavity 6541 and the fourth cavity 6542. The second cavity edge 6543 is embedded in the second groove portion 6511. The second cavity edge 6543 is provided with a third sheath fluid delivery microchannel 6544 communicating with the third sheath fluid pool 652 and a fourth sheath fluid delivery microchannel 6545 communicating with the fourth sheath fluid pool 653. The third cavity 6541 is embedded in the third receiving hole portion 6512 and communicates with the third sheath fluid delivery microchannel 6544. The fourth cavity 6542 is embedded in the fourth receiving hole portion 6513 and communicates with the fourth sheath fluid delivery microchannel 6545.

[0101] Wherein, the third sheath liquid delivery microchannel 6544 and the fourth sheath liquid delivery microchannel 6545, when the second sheath liquid container 654 is not pressed, the second sheath liquid in the second sheath liquid container 654 cannot enter the third sheath liquid pool 652 through the third sheath liquid delivery microchannel 6544, and the second sheath liquid in the second sheath liquid container 654 cannot enter the fourth sheath liquid pool 653 through the fourth sheath liquid delivery microchannel 6545, only when the second sheath liquid container 654 is pressed, the second sheath liquid in the second sheath liquid container 654 can enter the third sheath liquid pool 652 through the third sheath liquid delivery microchannel 6544, and the second sheath liquid in the second sheath liquid container 654 can enter the fourth sheath liquid pool 653 through the fourth sheath liquid delivery microchannel 6545.

[0102] The above merely describes the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any skilled person in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A microfluidic chip, characterized by, The chip comprises a chip body, the chip body is provided with: a first sample inlet; a second sample inlet; a third sample inlet; a first detection system in communication with the first sample inlet, the first detection system being configured to perform optical detection on a sample input from the first sample inlet; a second detection system in communication with the second sample inlet, the second detection system being configured to perform optical detection and impedance detection on a sample input from the second sample inlet; a third detection system in communication with the third sample inlet, the third detection system being configured to perform impedance detection on a sample input from the third sample inlet; a first driving channel, a first end of the first driving channel being in communication with the first detection system, a second end of the first driving channel being configured to connect a first driver, the first driver being configured to drive a liquid to move inside the first detection system through the first driving channel; a second driving channel, a first end of the second driving channel being in communication with the second detection system, a second end of the second driving channel being configured to connect a second driver, the second driver being configured to drive a liquid to move inside the second detection system through the second driving channel; a third driving channel, a first end of the third driving channel being in communication with the third detection system, a second end of the third driving channel being configured to connect a third driver, the third driver being configured to drive a liquid to move inside the third detection system through the third driving channel.

2. The microfluidic chip of claim 1, wherein, The samples input from the first sample inlet, the second sample inlet and the third sample inlet are all blood samples, the first detection system is configured to detect the blood samples to obtain immunoturbidimetric indexes of the blood samples, the second detection system is configured to detect the blood samples to obtain hemoglobin indexes and white blood cell classification indexes of the blood samples, and the third detection system is configured to detect the blood samples to obtain red blood cell indexes and platelet indexes of the blood samples; And / or, the first sample inlet, the second sample inlet and the third sample inlet are arranged adjacent to each other on the same side of the chip body, the microfluidic chip further comprises a blocking piece, the blocking piece being configured to be openably and closably connected to the chip body, and the blocking piece being configured to block the first sample inlet, the second sample inlet and the third sample inlet; And / or, the second ends of the first driving channel, the second driving channel and the third driving channel are arranged adjacent to each other on the same side of the chip body. And / or, the first detection system comprises a first buffer pool, a second buffer pool, a first mixing channel, a first reagent adding part, a first optical detection part and a second mixing channel, the first mixing channel is communicated with the first sample inlet and the first buffer pool, the first reagent adding part is communicated with the first sample inlet, the first reagent adding part is used for providing the first reagent mixed with the sample, the first optical detection part is communicated with the first reagent adding part, the first optical detection part is used for detecting the mixture of the sample and the first reagent, the second mixing channel is communicated with the first optical detection part and the second buffer pool, wherein the first end of the first driving channel is communicated with one of the first buffer pool and the second buffer pool, the first driver drives the sample and the first reagent to reciprocate in the first mixing channel and the second mixing channel by charging or sucking air to the first driving channel.

3. The microfluidic chip of claim 2, wherein, The chip body is further provided with a first sample quantifying part, a first sample conveying microchannel and a second sample conveying microchannel, the first sample quantifying part is communicated with the first sample inlet, the first sample quantifying part is used for quantifying the sample input into the first detection system from the first sample inlet, one end of the first sample conveying microchannel is communicated with the first sample quantifying part, the other end of the first sample conveying microchannel is communicated with the first mixing channel, one end of the second sample conveying microchannel is communicated with the first sample quantifying part, the other end of the second sample conveying microchannel is communicated with the first reagent adding part; And / or, the first reagent adding part comprises a first containing part, a first reagent pool and a first reagent container, the first sample inlet and the first optical detection part are communicated with the first reagent pool, the first reagent container is contained in the first containing part, the first reagent container stores the first reagent, the first reagent container injects the first reagent into the first reagent pool when being pressed, wherein the first reagent pool is arranged within the profile range of the first containing part; And / or, the chip body comprises a substrate, the substrate comprises a front surface and a back surface, one of the first mixing channel and the second mixing channel is arranged on the front surface of the substrate, the other of the first mixing channel and the second mixing channel is arranged on the back surface of the substrate.

4. The microfluidic chip of claim 3, wherein, The first containing part comprises a first sink part and a first through hole part, the first through hole part is arranged on the groove bottom surface of the first sink part and penetrates through the chip body, the first reagent pool is arranged on the groove bottom surface of the first sink part and is arranged in a spaced manner with the first through hole part, the first reagent container comprises a first cup body and a first cup edge, the first cup edge surrounds the periphery of the opening end of the first cup body, the first cup edge is embedded in the first sink part, the first cup edge is provided with a first reagent conveying microchannel communicated with the first reagent pool, the first cup body is embedded in the first through hole part and communicated with the first reagent conveying microchannel; And / or, the first mixing channel and the second mixing channel are arranged in a relative manner in the thickness direction of the substrate.

5. The microfluidic chip of claim 1, wherein, The second detection system comprises a first sheath flow part, a third buffer pool, a second reagent adding part, a second optical detection part, a third mixing channel, a first sheath liquid adding part, a first sheath liquid channel, a second sheath liquid channel and a first waste liquid pool. The first sheath flow part is provided with a first sample liquid inlet, a first sheath liquid inlet, a first auxiliary flow inlet, a second auxiliary flow inlet and a first waste liquid outlet. The third buffer pool is in communication with the second sample inlet. The second reagent adding part is in communication with the second sample inlet, and is used for providing a second reagent mixed with a sample. The second optical detection part is in communication with the second reagent adding part, and is used for detecting the mixture of the sample and the second reagent. The third mixing channel is in communication with the second optical detection part and the first sample liquid inlet. The first sheath liquid adding part is used for providing sheath liquid to the first sheath flow part. The first sheath liquid channel is in communication with the first sheath liquid adding part and the first sheath liquid inlet. One end of the second sheath liquid channel is in communication with the first sheath liquid adding part, and the other end of the second sheath liquid channel is in communication with the first auxiliary flow inlet and the second auxiliary flow inlet. The first waste liquid pool is in communication with the first waste liquid outlet, and is used for receiving waste liquid discharged by the first sheath flow part. The first end of the second driving channel is in communication with the third buffer pool, and the second driver drives the sample and the reagent to reciprocally flow in the third mixing channel by charging or sucking air into the second driving channel.

6. The microfluidic chip of claim 5, wherein, The chip body is further provided with a second sample quantifying part, a third sample conveying microchannel and a fourth sample conveying microchannel, the second sample quantifying part is in communication with the second sample inlet, and is used for quantifying the sample input into the second detection system from the second sample inlet, one end of the third sample conveying microchannel is in communication with the second sample quantifying part, the other end of the third sample conveying microchannel is in communication with the second reagent adding part, one end of the fourth sample conveying microchannel is in communication with the second sample quantifying part, and the other end of the fourth sample conveying microchannel is in communication with the third buffer pool. The second reagent adding part comprises a second containing part, a second reagent pool and a second reagent container, the second sample inlet and the second optical detection part are in communication with the second reagent pool, the second reagent container is contained in the second containing part, the second reagent container stores the second reagent, and the second reagent container injects the second reagent into the second reagent pool when being pressed, wherein the second reagent pool is arranged within the profile range of the second containing part. The chip body comprises a substrate, the substrate comprises a front surface and a back surface, the third mixing channel part is arranged on the front surface of the substrate, and the third mixing channel part is arranged on the back surface of the substrate. The first sheath flow part comprises a first gem hole, a first laminar flow groove and a first auxiliary flow groove, the first laminar flow groove and the first auxiliary flow groove are respectively located on two sides of the first gem hole, the first laminar flow groove, the first gem hole and the first auxiliary flow groove form an hourglass structure, the first sample liquid inlet and the first sheath liquid inlet are communicated with the first laminar flow groove, and the first auxiliary flow inlet, the second auxiliary flow inlet and the first waste liquid outlet are communicated with the first auxiliary flow groove. The chip body comprises a substrate, the substrate comprises a front surface and a back surface, the first sheath liquid channel and the second sheath liquid channel are arranged on the front surface of the substrate, and the first sheath flow part is arranged on the back surface of the substrate. The first sheath liquid adding part comprises a first accommodating part, a first sheath liquid pool, a second sheath liquid pool and a first sheath liquid container, the first sheath liquid channel is communicated with the first sheath liquid pool, the second sheath liquid channel is communicated with the second sheath liquid pool, the first sheath liquid container is accommodated in the first accommodating part, the first sheath liquid container stores the first sheath liquid, and the first sheath liquid container injects the first sheath liquid into the first sheath liquid pool and the second sheath liquid pool when being pressed.

7. The microfluidic chip of claim 6, wherein, The second accommodating part comprises a second sink part and a second through-hole part, the second through-hole part is arranged on the groove bottom surface of the second sink part and penetrates through the chip body, the second reagent pool is arranged on the groove bottom surface of the second sink part and is arranged in a spaced manner with the second through-hole part, the second reagent container comprises a second cup body and a second cup edge, the second cup edge surrounds the periphery of the opening end of the second cup body, the second cup edge is embedded in the second sink part, the second cup edge is provided with a second reagent delivery micro-channel communicated with the second reagent pool, and the second cup body is embedded in the second through-hole part and communicated with the second reagent delivery micro-channel. The third mixing channel arranged on the front surface of the substrate and the third mixing channel arranged on the back surface of the substrate are arranged in a relative manner in the thickness direction of the substrate. The first accommodating part comprises a first groove part, a first accommodating hole part and a second accommodating hole part, the first accommodating hole part and the second accommodating hole part are arranged on the groove bottom surface of the first groove part and penetrate through the chip body, the first sheath liquid pool and the second sheath liquid pool are arranged on the groove bottom surface of the first groove part, the first sheath liquid container comprises a first cavity, a second cavity and a first cavity edge, the first cavity edge surrounds the periphery of the opening end of the first cavity and the second cavity, the first cavity edge is embedded in the first groove part, the first cavity edge is provided with a first sheath liquid delivery micro-channel communicated with the first sheath liquid pool and a second sheath liquid delivery micro-channel communicated with the second sheath liquid pool, the first cavity is embedded in the first accommodating hole part and communicated with the first sheath liquid delivery micro-channel, and the second cavity is embedded in the second accommodating hole part and communicated with the second sheath liquid delivery micro-channel. The first sheath flow part further comprises a first flow guide part, a first branch flow channel and a second branch flow channel, the first flow guide part is arranged in the middle of the first laminar flow groove, the first flow guide part is provided with a first flow guide groove, one end of the first flow guide groove is communicated with the first sample liquid inlet, the other end of the first flow guide groove extends towards the first gem hole, the first sheath liquid inlet is arranged on the side of the first sample liquid inlet away from the first gem hole, one end of the first branch flow channel and one end of the second branch flow channel are communicated with the first sample liquid inlet, the other end of the first branch flow channel and the other end of the second branch flow channel are communicated with the first laminar flow groove, and the first flow guide part is located between the first branch flow channel and the second branch flow channel. The first sheath flow part further comprises a third branch flow channel and a fourth branch flow channel, one end of the third branch flow channel is communicated with the first auxiliary flow inlet, the other end of the third branch flow channel is communicated with the first auxiliary flow groove, one end of the fourth branch flow channel is communicated with the second auxiliary flow inlet, and the other end of the fourth branch flow channel is communicated with the first auxiliary flow groove, and the first gem hole is located between the third branch flow channel and the fourth branch flow channel. The microfluidic chip further comprises a first electrode and a second electrode, the first electrode is arranged in the first laminar flow groove, and the second electrode is arranged in the first auxiliary flow groove. The aperture of the first gem hole is 0.02mm to 1mm.

8. The microfluidic chip of claim 1, wherein, The third detection system comprises a second sheath flow part, a third reagent adding part, a fourth mixing channel, a fifth mixing channel, a second sheath liquid adding part, a third sheath liquid channel, a fourth sheath liquid channel and a second waste liquid pool. The second sheath flow part comprises a second sample liquid inlet, a second sheath liquid inlet, a third auxiliary flow inlet, a fourth auxiliary flow inlet and a second waste liquid outlet. The third reagent adding part is communicated with the third sample inlet, and the third reagent adding part is used for providing a third reagent mixed with a sample. The fourth mixing channel is communicated with the third reagent adding part. The fifth mixing channel is communicated with the third sample inlet and the second sample liquid inlet. The second sheath liquid adding part is used for providing a sheath liquid to the second sheath flow part. The third sheath liquid channel is communicated with the second sheath liquid adding part and the second sheath liquid inlet. One end of the fourth sheath liquid channel is communicated with the second sheath liquid adding part, and the other end of the fourth sheath liquid channel is communicated with the third auxiliary flow inlet and the fourth auxiliary flow inlet. The second waste liquid pool is communicated with the second waste liquid outlet, and the second waste liquid pool is used for receiving waste liquid discharged by the second sheath flow part. The first end of the third driving channel is communicated with the fourth mixing channel, and the third driver drives the sample and the reagent to flow back and forth in the fourth mixing channel and the fifth mixing channel by charging or sucking air in the third driving channel.

9. The microfluidic chip of claim 8, wherein, The chip body is further provided with a third sample quantifying part, a fifth sample conveying microchannel and a sixth sample conveying microchannel. The third sample quantifying part is in communication with the third sample inlet. The third sample quantifying part is used for quantifying the sample input from the third sample inlet into the third detection system. One end of the fifth sample conveying microchannel is in communication with the third sample quantifying part. The other end of the fifth sample conveying microchannel is in communication with the third reagent adding part. One end of the sixth sample conveying microchannel is in communication with the third sample quantifying part. The other end of the sixth sample conveying microchannel is in communication with the fifth mixing channel. And / or, the third reagent adding part comprises a third containing part, a third reagent pool and a third reagent container. The third reagent pool is in communication with the third sample inlet. The third reagent container is contained in the third containing part. The third reagent container stores the third reagent. The third reagent container injects the third reagent into the third reagent pool when pressed. The third reagent pool is arranged within the profile range of the third containing part. And / or, the chip body comprises a substrate. The substrate comprises a front surface and a back surface. The fourth mixing channel part is arranged on the front surface of the substrate. The fourth mixing channel part is arranged on the back surface of the substrate. And / or, the second sheath flow part comprises a second gem hole, a second laminar flow groove and a second auxiliary flow groove. The second laminar flow groove and the second auxiliary flow groove are respectively located on both sides of the second gem hole and are in communication with the second gem hole. The second laminar flow groove, the second gem hole and the second auxiliary flow groove form an hourglass structure. The second sample liquid inlet and the second sheath liquid inlet are in communication with the second laminar flow groove. The third auxiliary flow inlet, the fourth auxiliary flow inlet and the second waste liquid outlet are in communication with the second auxiliary flow groove. And / or, the chip body comprises a substrate. The substrate comprises a front surface and a back surface. The third sheath liquid channel and the fourth sheath liquid channel are arranged on the front surface of the substrate. The second sheath flow part is arranged on the back surface of the substrate. And / or, the second sheath liquid adding part comprises a second containing part, a third sheath liquid pool, a fourth sheath liquid pool and a second sheath liquid container. The third sheath liquid channel is in communication with the third sheath liquid pool. The fourth sheath liquid channel is in communication with the fourth sheath liquid pool. The second sheath liquid container is contained in the second containing part. The second sheath liquid container stores the second sheath liquid. The second sheath liquid container injects the second sheath liquid into the third sheath liquid pool and the fourth sheath liquid pool when pressed. The third sheath liquid pool and the fourth sheath liquid pool are arranged within the profile range of the second containing part.

10. The microfluidic chip of claim 9, wherein, The third accommodation part comprises a third sink part and a third through-hole part, the third through-hole part is arranged on the groove bottom surface of the third sink part and penetrates through the chip body, the third reagent pool is arranged on the groove bottom surface of the third sink part and is arranged in a spaced manner with the third through-hole part, the third reagent container comprises a third cup body and a third cup edge, the third cup edge is arranged around the opening end of the third cup body, the third cup edge is embedded in the third sink part, the third cup edge is provided with a third reagent delivery micro-channel in communication with the third reagent pool, and the third cup body is embedded in the third through-hole part and in communication with the third reagent delivery micro-channel; And / or, the fourth mixing channel located on the front surface of the substrate and the fourth mixing channel located on the back surface of the substrate are arranged in a thickness direction of the substrate in a facing manner; And / or, the chip body further comprises a cover plate, the cover plate covers the front surface of the substrate, and the fifth mixing channel is arranged on the cover plate; And / or, the second sheath flow part further comprises a second flow guide part, a fifth branch flow channel and a sixth branch flow channel, the second flow guide part is arranged in the middle part of the second laminar flow groove, the second flow guide part is provided with a second flow guide groove, one end of the second flow guide groove is in communication with the second sample liquid inlet, the other end of the second flow guide groove extends towards the second gem hole, the second sheath liquid inlet is arranged on one side of the second sample liquid inlet opposite to the second gem hole, one end of the fifth branch flow channel and one end of the sixth branch flow channel are in communication with the second sample liquid inlet, the other end of the fifth branch flow channel and the other end of the sixth branch flow channel are in communication with the second laminar flow groove, and the second flow guide part is located between the fifth branch flow channel and the sixth branch flow channel; And / or, the second sheath flow part further comprises a seventh branch flow channel and an eighth branch flow channel, one end of the seventh branch flow channel is in communication with the third auxiliary flow inlet, the other end of the seventh branch flow channel is in communication with the second auxiliary flow groove, one end of the eighth branch flow channel is in communication with the fourth auxiliary flow inlet, and the other end of the eighth branch flow channel is in communication with the second auxiliary flow groove, and the second gem hole is located between the seventh branch flow channel and the eighth branch flow channel; And / or, the second accommodation part comprises a second groove part, a third accommodation hole part and a fourth accommodation hole part, the third accommodation hole part and the fourth accommodation hole part are arranged on the groove bottom surface of the second groove part and penetrate through the chip body, the third sheath liquid pool and the fourth sheath liquid pool are arranged on the groove bottom surface of the second groove part, the second sheath liquid container comprises a third cavity, a fourth cavity and a second cavity edge, the second cavity edge is arranged around the opening end of the third cavity and the fourth cavity, the second cavity edge is embedded in the second groove part, the second cavity edge is provided with a third sheath liquid delivery micro-channel in communication with the third sheath liquid pool and a fourth sheath liquid delivery micro-channel in communication with the fourth sheath liquid pool, the third cavity is embedded in the third accommodation hole part and in communication with the third sheath liquid delivery micro-channel, and the fourth cavity is embedded in the fourth accommodation hole part and in communication with the fourth sheath liquid delivery micro-channel; And / or, the microfluidic chip further comprises a third electrode and a fourth electrode, the third electrode is arranged in the second layer flow channel, and the fourth electrode is arranged in the second auxiliary flow channel. And / or, the second gem hole has a pore size of 0.02mm to 1mm.

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