Reagent disc for analyzing sample containing component

Abstract

The utility model relates to a reagent disc for analyzing a sample containing components, which comprises a disc body, the disc body is provided with an annular working area, and the annular working area is provided with a plurality of reaction cups, a plurality of reagent cups 5, a plurality of liquid containing cups and a sample cup along the annular track of the annular working area. The device is characterized in that a plurality of counting chambers are further arranged in the annular working area along the annular track of the annular working area, the counting chambers are used for quantitatively containing solutions containing forming components, and the device has the effect of facilitating analysis and counting of the forming components in samples such as whole blood and quantitative detection of intangible components.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro diagnostic detection, in particular to a reagent disc for analyzing a sample containing formed elements. BACKGROUND

[0002] In the field of in vitro diagnosis, it is often necessary to analyze the morphology and count the formed elements in a sample containing formed elements, and to quantitatively analyze the content of the non-formed elements in the sample, so as to achieve the functions of preventing diseases, assisting diagnosis, monitoring treatment effects, etc.

[0003] Taking a whole blood sample as an example, the formed elements in the sample include red blood cells, white blood cells, platelets, etc., and the non-formed elements are of various types, and the indicators that need to be detected in clinical practice include hemoglobin, C-reactive protein, etc. The formed elements such as red blood cells, white blood cells, and platelets need to be observed for their morphology, size, quantity, etc., and the non-formed element analysis needs to detect the concentration of hemoglobin and the concentration of C-reactive protein.

[0004] The current commonly used methods for analyzing the formed elements in a sample include manual counting method and Coulter principle instrument detection method. The Coulter principle refers to that when particles suspended in an electrolyte pass through a small hole tube, the particles replace the same volume of electrolyte, causing an instantaneous change in the resistance between the two electrodes inside and outside the small hole tube in a constant current designed circuit, and generating a potential pulse. The size and number of pulse signals are proportional to the size and number of particles. The product applied more in the Coulter principle cell counting method is a blood cell analyzer, which has the advantages of accurate results, good repeatability, and simple operation. The manual counting method mainly adopts the principle of bovine counting, and the detection personnel first mix the sample to be tested with a cell diluent, then add the diluted cells to a counting plate, fill the counting pool by means of liquid capillary phenomenon, and the cells in the sample are deposited on the bottom surface of the counting pool, and the detection personnel observe the morphology of the cells deposited on the bottom surface of the counting plate and count them under a microscope.

[0005] However, the Coulter principle instrument (sample analyzer) has the disadvantages of complex structure, expensive equipment, and large volume of reagents, and the manual counting method has the defects of tedious operation, low efficiency, and low quality.

[0006] In order to effectively solve the defects of the above two methods, it is urgent to develop a device suitable for observing and counting the formed elements and quantitatively detecting the non-formed elements in a sample containing formed elements such as whole blood, so that no complex and expensive instrument is needed during detection, and no tedious manual operation is needed, and suitable equipment is provided for primary medical institutions, bedside, pet hospitals, etc. CONTENT OF THE INVENTION

[0007] To facilitate the analysis and counting of formed elements and the quantitative detection of intangible components in samples such as whole blood, this application provides a reagent tray for the analysis of samples containing formed elements.

[0008] The reagent tray for analyzing samples containing formed elements provided in this application adopts the following technical solution:

[0009] A reagent tray for analyzing samples containing constituent elements includes a tray body, on which an annular working area is provided. Along its annular trajectory, the annular working area is provided a reaction cup, several reagent cups 5, several liquid-containing cups, and a sample cup. The annular working area is characterized by having several counting cells along its annular trajectory, the counting cells being used for quantitatively dispensing solutions containing constituent elements.

[0010] By adopting the above technical solution, the sample cup, reagent cup 5, reaction cup, and liquid container are arranged within the annular working area of ​​the disc body. This allows the sample, reaction cup, reagents, diluent, and cleaning solution required for the detection of formed and intangible components to be contained within a single annular working area. The disc body can be flexibly manipulated above this area by rotating its circumference. The accompanying analyzer only needs to move its pipette up and down above the annular working area to perform sample and reagent aspiration, mixing, transfer, and cleaning. Several counting cells are also arranged along the annular trajectory of the working area. These counting cells are used to quantitatively dissolve solutions containing formed components, allowing them to be used for filling reagent-treated solutions containing formed components. The accompanying analyzer only needs to install a vertically movable microscope camera above the annular working area to perform microscopic imaging of the formed components within the counting cells, thereby achieving morphological analysis and counting of the formed components in the sample. The integration of these elements significantly reduces reliance on the instrument's mechanical structure, simplifies the instrument's structure, eliminates the need for liquid circuits, and enables miniaturization and simplified operation.

[0011] Preferably, the counting cell includes a cavity and a cover plate, the cover plate covering the cavity, and the depth of the cavity is set between 0.05 mm and 0.5 mm.

[0012] Preferably, the cavity and the cover plate are integrally formed, and the cover plate is hinged to the cavity.

[0013] Preferably, the cavity and the cover plate are separate components.

[0014] Preferably, the disc body is further provided with a sealing element, which is connected in a sealed manner to the reaction cup, reagent cup 5, liquid container cup, and liquid container.

[0015] By adopting the technical scheme, the disc body is provided with a sealing element, the sealing element is in sealing connection with the reaction cup, the reagent cup 5 and the liquid containing cup, the sealing of the liquid such as reagent, diluent and cleaning liquid pre-filled in the reaction cup, the reagent cup 5 and the liquid containing cup is facilitated, and the reagent disc is convenient to store and transport.

[0016] Preferably, the disc body is provided with a connecting structure for detachable connection with a matched sample analyzer.

[0017] By adopting the technical scheme, the disc body is installed on the detection equipment, the stability of the disc body placed on the detection equipment is improved, and the probability of the disc body moving, falling off or shaking along the detection equipment during the working of the detection equipment is reduced.

[0018] Preferably, the reaction cup is used for optical colorimetry, and the reaction cup is provided as a transparent material reaction cup.

[0019] By adopting the technical scheme, the absorbance colorimetry of the solution in the reaction cup is facilitated, and the measurement of the concentration of the non-forming component in the sample is realized.

[0020] In summary, the present application has at least one of the following beneficial technical effects:

[0021] 1. The sample cup, the reagent cup 5, the reaction cup, the liquid containing cup and the counting pool are arranged in the annular working area of the disc body, so that the sample, the reaction cup, the reagent, the diluent, the cleaning liquid and the counting pool required for the detection of the sample forming component and the non-forming component are arranged in the annular working area of one disc body, and can be flexibly controlled and used above the annular working area through the rotation of the circumference of the disc body. The matched analyzer only needs to move the pipette and the microscope lens up and down above the annular working area to realize the suction, mixing, transfer, cleaning and morphological analysis and counting of the sample and the reagent, greatly simplifies the instrument structure, eliminates the liquid path, realizes the miniaturization of the instrument, and achieves the effects of simple operation, detection automation and the like.

[0022] 2. The bottom plate and the cover plate of the counting pool are integrally formed, which can simplify the operation steps, improve the detection efficiency and improve the detection quality.

[0023] 3. The disc body is provided with a sealing element, the sealing element is in sealing connection with the reaction cup, the reagent cup 5 and the liquid containing cup, the sealing of the liquid such as reagent, diluent and cleaning liquid pre-filled in the reaction cup, the reagent cup 5 and the liquid containing cup is facilitated, and the reagent disc is convenient to store and transport. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is a whole structure schematic view of a reagent disc for sample analysis according to an embodiment of the present application;

[0025] Figure 2The embodiment of the present application mainly embodies the schematic diagram of the counting pool structure.

[0026] Marked with 1, disc body; 2, circular working area; 3, reaction cup; 4, liquid containing cup; 5, reagent cup; 6, sample cup; 7, counting pool; 71, cavity; 72, cover plate. DETAILED DESCRIPTION

[0027] The following will be combined with the attached Figure 1 The present application is further described in detail.

[0028] The embodiment of the present application discloses a reagent disc for sample analysis. Referring to Figure 1 A reagent disc for sample analysis comprises a disc body 1, the disc body 1 is provided with a circular working area 2, a plurality of reaction cups 3, a plurality of reagent cups 5, a plurality of liquid containing cups 4 and a reagent cup 6 are arranged on the circular working area 2 along the circular track thereof, and the circular working area 2 is further provided with a plurality of counting pools 7 along the circular track thereof, the counting pools 7 are used for containing formed components in the solution.

[0029] Specifically, the reaction cup 3 is used for containing the acellular components in the whole blood sample, the reaction cup 3 is used for reacting the formed components with the detection reagent and the acellular components, the reagent cup 5 is used for containing the detection reagent, the liquid containing cup 4 is used for cleaning the reagent disc to prevent the inaccurate results caused by cross infection when the detection equipment detects different components, and the reagent cup 6 is provided with at least one and used for containing the whole blood sample. The number of each type can be increased or decreased according to actual needs and the size of the reagent disc, and the specific number of each type is not described herein.

[0030] Correspondingly, the positions and the sequence of the reaction cup 3, the reagent cup 5, the liquid containing cup 4, the reagent cup 6 and the counting pool 7 can be adjusted according to the detection needs. In the embodiment, in order to better illustrate them, the reaction cup 3, the liquid containing cup 4, the reagent cup 5, the reagent cup 6 and the counting pool 7 are sequentially arranged in the clockwise direction along the circular working area 2, and in order to facilitate the description, the local diagram of the disc body 1 is taken in the attached drawings.

[0031] Therefore, the whole blood sample is contained by the reagent cup 6, then the whole blood sample is processed by the detection equipment and the detection of the acellular components is completed, at the same time, because the counting pool 7 is arranged on the circular working area 2, when the processing of the whole blood sample is completed, the formed components in the processed whole blood sample can be directly added into the counting pool 7, and the detection of the formed components in the whole blood sample can be completed by the detection equipment, without the need of separating the formed components and the acellular components in the whole blood sample, and then transferring the formed components to other reaction disc for detection, the operation is simple, and the detection efficiency of each component of the whole blood sample is improved.

[0032] Further, the counting pool 7 comprises a cavity 71 and a cover plate 72 (not shown in the figure), the cavity 71 is recessed along the thickness direction of the annular working area 2, and the cover plate 72 covers the cavity 71, the depth of the cavity 71 is set between 0.05 mm and 0.5 mm, so that the cavity 71 has good light transmittance and meets the accommodation requirements of the cavity 71 to the whole blood sample or reagent. At the same time, in the embodiment, the cavity 71 is set as a regular geometric shape, such as a circle, a rectangle, a polygon and the like, and the amount of the sample or reagent in the cavity 71 can be quickly calculated by using the basic mathematical formula, which is simple to operate.

[0033] In one embodiment, the cavity 71 and the cover plate 72 are integrally formed, and the cover plate 72 is hinged to the cavity 71, that is, the cover plate 72 and the cavity 71 can be formed by injection molding without separately processing the cover plate 72 and the cavity 71, and then when the counting pool 7 is used, since the cover plate 72 is hinged to the cavity 71, the cover plate 72 only needs to be opened along the cavity 71, which is beneficial to improve the production efficiency of the reagent disc.

[0034] In another embodiment, the cavity 71 and the cover plate 72 are separately provided, that is, the cover plate 72 is mass-produced, and then the cover plate 72 is respectively covered on the corresponding cavity 71, which is easy to process and form.

[0035] At the same time, in the above embodiment, the cavity 71 and the cover plate 72 are made of transparent material (for example, PVC material, acrylic material, silica gel material), which improves the light transmittance of the counting pool 7 and reduces the probability of inaccurate sample detection in the counting pool 7 due to insufficient light.

[0036] At the same time, the disc body 1 is also provided with a sealing member (not shown in the figure), which seals the reaction cup 3, the reagent cup 5, the liquid cup 4, the reagent cup 6 and the counting pool 7, and is convenient for pre-filling (such as detection reagent or solvent), which is beneficial to store and / or transport the disc body 1 filled with reagent.

[0037] The sealing member can be an aluminum foil sealing film, a silica gel sealing film, a plastic sealing film or a rubber sealing film.

[0038] In addition, the disc body 1 is provided with a connecting structure (not shown in the figure) for detachable connection with a matched sample analyzer. The connecting structure can be a clamping mode (for example, a clamping groove is formed on the matched sample analyzer, and a clamping piece is arranged on the end of the disc body 1 abutting against the matched sample analyzer, and the clamping piece is clamped and matched with the clamping groove), a bolt fixing mode and a magnetic attraction connection (for example, a magnetic block is arranged on the disc body 1, and a metal piece that can be magnetically attracted to the magnetic block is arranged on the matched sample analyzer, and when the disc body 1 is arranged on the matched sample analyzer, the magnetic block and the metal piece are magnetically attracted to each other), so that the disc body 1 can be stably installed on the matched sample analyzer, and the probability of movement or falling of the disc body 1 along the detection equipment during the working of the detection equipment is reduced.

[0039] Further, the reaction cup 3 is used for optical colorimetry, and the reaction cup 3 is a transparent material reaction cup, which facilitates the absorbance colorimetry of the solution in the reaction cup 3, so as to realize the measurement of the concentration of the non-forming part in the sample.

[0040] The implementation principle of the reagent disc for sample analysis is that the disc body 1 is provided with the reaction cup 3, the reagent cup 5, the liquid container cup 4, the reagent cup 6 and the counting pool 7, which cooperate with each other to realize the purpose of simultaneously containing the formed part and the non-formed part in the whole blood sample, without the need of transferring the formed part and the non-formed part in the whole blood sample to other containers after the separation of the formed part and the non-formed part in the whole blood sample, so as to realize the purpose of simultaneously containing the formed part and the non-formed part in the whole blood sample.

[0041] The above are preferred embodiments of the present application, and are not intended to limit the protection scope of the present application, so that: any equivalent changes made according to the structure, shape, principle of the present application shall be covered within the protection scope of the present application.

Claims

1. A reagent tray for analyzing samples containing constituent components, comprising a tray body (1), wherein a circular working area (2) is provided on the tray body (1), and reaction cups (3), a plurality of reagent cups (5), a plurality of liquid-containing cups (4), and sample cups (6) are arranged along the circular trajectory of the circular working area (2), characterized in that: The circular annular working area (2) is further provided with a plurality of counting pools (7) along its circular annular track, and the counting pools (7) are used for quantitatively accommodating a solution containing a forming component.

2. The reagent disc for containing a sample analysis of a formed component according to claim 1, wherein: The counting pool (7) comprises a cavity (71) and a cover plate (72), and the cover plate (72) covers the cavity (71), and the depth of the cavity (71) is set to be between 0.05mm and 0.5mm.

3. The reagent disc for containing a sample analysis of a formed component according to claim 2, wherein: The cavity (71) and the cover plate (72) are integrally formed.

4. The reagent disc for containing a sample analysis of a formed component according to claim 3, wherein: The cavity (71) and the cover plate (72) are separately provided.

5. The reagent disc for containing a sample analysis of a formed component according to claim 1, wherein: The disc body (1) is further provided with a sealing member, and the sealing member is sealingly connected with the reaction cup (3), the reagent cup (5) and the liquid containing cup (4).

6. The reagent disc for containing a sample analysis of a forming component according to any one of claims 1-5, wherein: The disc body (1) is provided with a connecting structure for detachably connecting with a matched sample analyzer.

7. The reagent disc for containing a sample analysis of a formed component according to claim 1, wherein: The reaction cup (3) is used for optical colorimetry, and the reaction cup (3) is provided as a transparent material reaction cup.

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