Positive and negative pressure rotary biological culture device

Abstract

The utility model discloses a positive and negative pressure rotary biological culture device, which belongs to the technical field of biological sample experimental equipment and comprises a control host, an environment adjusting system and a rotary culture system arranged in the environment adjusting system. The spherical bottom culture bottle is arranged on the rotating mechanism, so that the spherical bottom culture bottle is driven by the rotating mechanism to rotate. By adopting the positive and negative pressure rotary biological culture device, a stable culture environment is provided for biological sample development required by different environments, different culture environment selections are provided for biological culture research, meanwhile, the nutrient exchange rate of biological cells can be increased, and the phenomena of slow development or death of the biological sample cells are reduced.

Description

Technical Field

[0001] This utility model relates to the field of biological sample experimental equipment technology, and in particular to a positive and negative pressure rotating biological culture device. Background Technology

[0002] In the field of biological sample culture, traditional static culture methods (where biological samples are statically suspended in nutrients or adsorbed onto the inner wall of a culture vessel) can lead to delayed cell development or cell death when biological sample cells develop into cell aggregates due to insufficient nutrient exchange between cells and the culture medium and the culture environment not meeting the requirements of the original organism.

[0003] Meanwhile, for biological sample cells with special environmental requirements, there are specific requirements for environmental pressure (air pressure or water pressure) during the developmental stage:

[0004] For example, mouse embryonic cells develop and grow in the mother mouse's uterus. As the mouse embryonic cells develop from a fertilized egg to a blastocyst, they require a low-oxygen, normobaric, and isothermal developmental environment. When the blastocyst cells develop into cell aggregates (the stage with a heartbeat), they require a high-pressure, high-oxygen, and isothermal developmental environment. During the development of mouse embryonic cells, it can be clearly observed that the developmental environment changes from the initial low-oxygen, normobaric developmental environment to a high-oxygen, high-pressure developmental environment.

[0005] For example, aquatic plant sample cells grow in a high-pressure, low-oxygen environment, and traditional static culture methods cannot provide the high-pressure (air pressure or water pressure) environment required for the germination and development of aquatic plant sample cells.

[0006] For example, high-altitude plant sample cells grow in a low-pressure and low-oxygen environment, and traditional static culture methods cannot provide the low-pressure development environment required for the growth and development of high-altitude plant sample cells.

[0007] Therefore, traditional static culture methods are not suitable for culturing biological samples that require nutrient exchange, gas (water) pressure, and oxygen and carbon dioxide concentrations. Static culture methods cannot simulate the cellular state under in vivo physiological conditions, and the growth environment they provide cannot enable the cultured samples to exhibit functions similar to those under the developmental conditions of intact plants, animals, or humans.

[0008] Patent application number CN202210264642.7 discloses a deep-sea microbial isolation and culture device and culture method, and patent application number CN201880083597.7 discloses a Xi Baoping biological culturer with variable internal pressure. The above patents only realize the adjustment of pressure, temperature and humidity, which belongs to static culture method. They do not solve the technical problem that when biological sample cells develop into cell aggregates, the insufficient nutrient exchange between cells and culture medium and the culture environment not meeting the requirements of the original organism will lead to slow development or death of biological sample cells. Utility Model Content

[0009] The purpose of this invention is to provide a positive and negative pressure rotating biological culture device that can solve the problem that static culture methods cannot truly simulate the developmental environment in vivo, especially in in vitro culture experiments involving spheroids, organoids and other cell aggregates.

[0010] To achieve the above objectives, this utility model provides a positive and negative pressure rotating biological culture device, including a control host and an environmental control system, and a rotating culture system disposed within the environmental control system. The rotating culture system includes a rotating mechanism and at least one spherical bottom culture bottle, which is disposed on the rotating mechanism so that the spherical bottom culture bottle rotates under the drive of the rotating mechanism.

[0011] Preferably, the spherical bottom culture flask includes a spherical culture section and a cylindrical connecting section. The spherical culture section is used to hold biological samples. One end of the cylindrical connecting section is connected to the spherical culture section, and the other end of the cylindrical connecting section is provided with a detachable connecting section. When the biological samples and nutrient solutions are placed in the spherical bottom culture flask, the liquid level of the biological samples and nutrient solutions is lower than the lowest point of the cylindrical connecting section when the spherical bottom culture flask is installed horizontally.

[0012] Preferably, the rotating mechanism includes a rotary drive motor for providing rotational power. The rotary drive motor is connected to a gas distribution plate via a rotary joint. The rotary joint is connected to an environmental control system, so that when the rotary drive motor drives the gas distribution plate to rotate, it simultaneously performs gas delivery, pressure regulation, and temperature and humidity regulation. Several gas distribution blocks are connected to the gas distribution plate. Each gas distribution block is provided with a connecting seat, which is connected to a detachable connecting section. Each gas distribution block is provided with an inlet pipe and a return pipe for gas exchange within the spherical bottom culture bottle. The tail end of the inlet pipe is positioned opposite to the spherical culture section, and the tail end of the return pipe is located within the cylindrical connecting section. The rotary drive motor is connected to a main control unit for regulating the rotational speed of the gas distribution plate.

[0013] Preferably, the environmental control system includes a gas positive and negative pressure control subsystem, a temperature control subsystem, a concentration control subsystem, and a humidity control subsystem;

[0014] The concentration control subsystem is connected to the positive and negative pressure control subsystem, which in turn is connected to the humidity control subsystem. The humidity control subsystem is also connected to the rotary joint. The rotary joint is connected to the positive and negative pressure control subsystem.

[0015] The gas positive and negative pressure control subsystem, temperature control subsystem, concentration control subsystem, and humidity control subsystem are all electrically connected to the main control unit and are used to regulate the culture environment of the spherical bottom culture flask.

[0016] Preferably, the gas positive and negative pressure control subsystem is used to provide a positive pressure, negative pressure, or normal pressure biological sample development environment for biological culture;

[0017] The gas positive and negative pressure control subsystem includes a switching valve and a positive and negative pressure generator. The switching valve is sequentially equipped with a mixed gas inlet, an atmospheric pressure inlet, a pressure regulating input inlet, and an output inlet. The mixed gas inlet is connected to the output of the concentration control subsystem, which in turn is connected to the positive and negative pressure generator via a mixed gas inlet pipeline. The atmospheric pressure inlet is open to air. The pressure regulating input inlet is connected to the output of the positive and negative pressure generator, and the output inlet is connected to the input of the humidity control subsystem. The return gas pipe is connected to the input of the positive and negative pressure generator via a return gas inlet pipeline.

[0018] Both the switching valve and the positive and negative pressure generator are electrically connected to the control host.

[0019] Preferably, the gas positive and negative pressure subsystem keeps the mixed gas in the gas path in a normal pressure state, a positive pressure state, or a negative pressure state according to the set pressure parameters.

[0020] The gas positive and negative pressure control process is as follows:

[0021] Atmospheric pressure process:

[0022] The switching valve opens the mixed gas inlet, the atmospheric pressure inlet, the pressure regulating input inlet, and the output inlet. The positive and negative pressure generator opens the return gas inlet and closes the mixed gas inlet. The positive and negative pressure generator connects the return gas inlet to the pressure regulating inlet of the switching valve. The mixed gas passing through the rotary culture system flows out into the air through the atmospheric pressure inlet of the switching valve. The gas pressure of the mixed gas in the rotary culture system is at atmospheric pressure.

[0023] Positive pressure process:

[0024] The switching valve closes the mixed gas inlet and the atmospheric pressure inlet, and opens the pressure regulating input inlet and the output inlet. The positive and negative pressure generator closes the return gas inlet and opens the mixed gas inlet. The positive and negative pressure generator pressurizes the gas in the rotary culture system.

[0025] Negative pressure state process:

[0026] The switching valve opens the mixed gas inlet, the atmospheric pressure inlet, the pressure regulating input inlet, and the output inlet. The positive and negative pressure generator opens the return gas pipeline and the mixed gas pipeline, and the positive and negative pressure generator performs negative gas operation on the rotary culture system.

[0027] Preferably, the temperature control subsystem includes a constant temperature chamber, a rotary culture system is installed inside the constant temperature chamber to provide a stable temperature development environment, a chamber temperature control unit is installed inside the constant temperature chamber, a door temperature control unit is installed on the door of the constant temperature chamber, and a humidifier bottle temperature control unit is installed on the side of the constant temperature chamber to provide a stable temperature environment for the humidifier bottle. The chamber temperature control unit, the door temperature control unit, and the humidifier bottle temperature control unit all include an electric heating plate and a temperature sensor. The heating plate inside the constant temperature chamber is installed on the inner wall of the constant temperature chamber, and the electric heating plate and the temperature sensor are electrically connected to the control host.

[0028] Preferably, the concentration control subsystem is used to provide a stable gas concentration development environment for biological culture;

[0029] The concentration control subsystem includes an air intake control component. The input end of the air intake control component is equipped with an air inlet, an oxygen inlet, a carbon dioxide inlet, and a nitrogen inlet. The output end of the air intake control component is connected to a fan pump, which provides circulation power for the gas flow in the gas path. A gas concentration detection component is set between the output end of the air intake control component and the fan pump to detect the concentration of the corresponding gas in the output gas.

[0030] The intake control unit, fan pump, and gas concentration detection unit are all electrically connected to the control host.

[0031] Preferably, the concentration control subsystem mixes the corresponding gases to form a mixed gas according to the set concentration parameters;

[0032] The gas concentration detection component detects the gas concentration of the corresponding gas in the gas path, compares the detected gas concentration with the set gas concentration parameter, and feeds it back to the control host. The control host adjusts the intake control component accordingly to supplement the corresponding gas until the gas concentration of the corresponding gas reaches the set concentration.

[0033] The oxygen concentration adjustment procedure is as follows:

[0034] Open the air vent and nitrogen vent, and close the oxygen vent. Reduce the oxygen concentration by continuously supplying nitrogen to expel oxygen from the gas path in the concentration control subsystem. The oxygen concentration should be less than 210,000 ppm.

[0035] Close the air vent and nitrogen vent, and open the oxygen vent. By continuously supplying oxygen, the oxygen concentration in the gas circuit of the concentration control subsystem is increased to a level greater than 210,000 ppm.

[0036] Close the oxygen and nitrogen vents and open the air vent to continuously supply air to increase the oxygen concentration in the gas path of the concentration control subsystem. The oxygen concentration is equal to 210,000 ppm.

[0037] The carbon dioxide adjustment procedure is as follows:

[0038] Close the carbon dioxide vent and open the air or nitrogen vent to continuously introduce air or nitrogen into the gas path of the concentration control subsystem to reduce the carbon dioxide concentration.

[0039] Open the carbon dioxide vent to continuously introduce carbon dioxide into the gas path of the concentration control subsystem, thereby increasing the carbon dioxide concentration.

[0040] Preferably, the humidity control subsystem is used to provide a humidity environment with a set humidity for biological culture; the humidity control subsystem includes a humidifier bottle and a humidity detection component installed on the return gas pipe, the humidity detection component is used to detect the humidity of the mixed gas flowing out after passing through the spherical bottom culture bottle; the input end of the humidifier bottle is connected to the output vent, and the output end of the humidifier bottle is connected to a rotary joint.

[0041] Both the humidifier bottle and the humidity detection component are electrically connected to the control unit;

[0042] The mixed gas is humidified by the humidifier bottle and then enters the spherical bottom culture bottle in the rotating culture system. The humidity is detected by the humidity detection component on the return gas pipe, and the working power of the humidifier bottle is adjusted according to the detected humidity data and the set humidity.

[0043] Therefore, the above-mentioned positive and negative pressure rotating biological culture device has the following beneficial effects:

[0044] (1) A rotating culture system is set up, with the spherical bottom culture bottle set on the rotating mechanism, so that the spherical bottom culture bottle rotates under the drive of the rotating mechanism. By adopting the rotating culture method, the exchange of nutrients and the discharge of waste in the biological sample during the culture process are improved. Moreover, the use of the spherical bottom culture bottle ensures that the culture sample will not be subjected to excessive impact during the rotating culture process, and will not affect the survival rate of the culture sample.

[0045] (2) The environmental control system includes a gas positive and negative pressure control subsystem, a temperature control subsystem, a concentration control subsystem, and a humidity control subsystem. It provides multiple combinations of developmental environments to meet the different requirements of biological sample development, including positive pressure, negative pressure, and normal pressure biological sample development environments; high oxygen concentration development environments, low oxygen concentration development environments, high carbon dioxide concentration development environments, and low carbon dioxide concentration development environments; and different humidity development environments. It can simulate the in vivo developmental environment of organisms, promote the growth of 3D cell structures (including spheroids, organoids, and other cell aggregates), and maintain and restore their in vivo functionality.

[0046] The technical solution of this utility model will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0047] Figure 1 This is a three-dimensional structural diagram of a positive and negative pressure rotating biological culture device according to this utility model;

[0048] Figure 2 This is a schematic diagram of the rotating culture system of this utility model;

[0049] Figure 3 This is a block diagram of the gas positive and negative pressure control subsystem and the concentration control subsystem of this utility model;

[0050] Figure 4 This is a block diagram of the control principle of the concentration control subsystem of this utility model;

[0051] Figure 5 This is a block diagram illustrating the control principle of a positive and negative pressure rotating biological culture device according to this utility model.

[0052] Figure Labels

[0053] 1. Rotary culture system; 11. Rotary mechanism; 111. Gas distribution plate; 112. Gas distribution block; 113. Threaded connector; 114. Rotary joint; 115. Rotary drive motor; 116. Air inlet pipe; 117. Air return pipe; 12. Spherical bottom culture flask; 121. Spherical culture section; 122. Cylindrical connector; 123. Connecting threaded section;

[0054] 2. Environmental Control System; 21. Gas Positive and Negative Pressure Control Subsystem; 211. Positive and Negative Pressure Generator; 211a. Mixed Gas Ventilation Pipeline; 211b. Return Gas Ventilation Pipeline; 212. Switching Valve; 212a. Mixed Gas Vent; 212b. Atmospheric Pressure Vent; 212c. Pressure Regulating Input Vent; 212d. Output Vent; 22. Temperature Control Subsystem; 221. Door Temperature Control Unit; 222. Cabin Temperature Control Unit; 223. Humidifier Bottle Temperature Control Unit; 22a. Heating Plate; 23. Concentration Control Subsystem; 231. Air Intake Control Component; 231a. Air Vent; 231b. Oxygen Vent; 231c. Carbon Dioxide Vent; 231d. Nitrogen Vent; 232. Gas Concentration Detection Component; 233. Fan Pump; 24. Humidity Control Subsystem; 241. Humidifier Bottle; 242. Humidity Detection Component;

[0055] 3. Control host. Detailed Implementation

[0056] Example

[0057] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0058] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0059] like Figure 1 As shown, a positive and negative pressure rotating biological culture device includes a control host 3, an environmental control system 2, and a rotating culture system 1 installed within the environmental control system 2.

[0060] like Figure 2As shown, the rotary culture system 1 includes a rotary mechanism 11 and at least one spherical-bottom culture bottle 12. This embodiment has six circumferentially distributed spherical-bottom culture bottles 12. The number of spherical-bottom culture bottles 12 and their arrangement can be adjusted according to actual needs. Each spherical-bottom culture bottle 12 includes a spherical culture section 121 and a cylindrical connecting section 122. The spherical culture section 121 holds biological samples. One end of the cylindrical connecting section 122 is connected to the spherical culture section 121, and the other end of the cylindrical connecting section 122 has a connecting thread section 123. When the biological sample and nutrient solution are placed inside the spherical-bottom culture bottle 12, and the spherical-bottom culture bottle 12 is installed horizontally, the liquid level of the biological sample and nutrient solution is lower than the lowest point of the cylindrical connecting section 122, ensuring that the biological sample and nutrient solution remain within the spherical culture section 121 during rotation.

[0061] A spherical-bottom culture flask 12 is mounted on a rotating mechanism 11, causing the spherical-bottom culture flask 12 to rotate under the drive of the rotating mechanism 11. The rotating mechanism 11 includes a rotary drive motor 115 for providing rotational power. The rotary drive motor 115 is connected to a gas distribution plate 111 via a rotary joint 114. The rotary joint 114 is connected to an environmental control system 2, so that when the rotary drive motor 115 drives the gas distribution plate 111 to rotate, it simultaneously performs gas delivery, pressure regulation, and temperature and humidity regulation. Several gas distribution blocks 112 are circumferentially distributed on the gas distribution plate 111. Threaded connecting seats 113 are provided on the gas distribution blocks 112, and the threaded connecting seats 113 are connected to... The connecting thread section 123 is threaded, and the gas distribution block is equipped with an inlet pipe 116 and a return pipe 117 for gas exchange within the spherical bottom culture flask 12. The tail end of the inlet pipe 116 is positioned opposite to the spherical culture section 121, and the tail end of the return pipe 117 is located within the cylindrical connecting part 122. The flask is installed using a threaded tightening method, ensuring that the mounting point between the spherical bottom culture flask 12 and the rotating mechanism 11 can withstand high-pressure or negative-pressure gas without leakage, while also ensuring that the spherical bottom culture flask 12 does not loosen under high-pressure gas conditions. Alternatively, a snap-fit ​​connection or an interference fit connection with the silicone stopper can be used depending on actual needs, both of which allow for detachable connections.

[0062] The rotary drive motor 115 is electrically connected to the control host 3 and is used to regulate the rotation speed of the gas distribution plate 111. The gas distribution plate 111 drives the six spherical bottom culture bottles 12 to rotate synchronously.

[0063] By employing a rotation culture method, the exchange of nutrients and the removal of waste products in biological samples during the culture process are improved. Furthermore, the use of a spherical-bottomed culture flask 12 ensures that the cultured samples are not subjected to excessive impact during the rotation culture process, thus not affecting the survival rate of the cultured samples.

[0064] The environmental control system 2 includes a gas positive and negative pressure control subsystem 21, a temperature control subsystem 22, a concentration control subsystem 23, and a humidity control subsystem 24. The concentration control subsystem 23 is connected to the positive and negative pressure control subsystem, which is connected to the humidity control subsystem 24. The humidity control subsystem 24 is connected to the rotary joint 114. The rotary joint 114 is connected to the positive and negative pressure control subsystem. The gas positive and negative pressure control subsystem 21, temperature control subsystem 22, concentration control subsystem 23, and humidity control subsystem 24 are all electrically connected to the control host 3 and are used to regulate the culture environment of the spherical bottom culture flask 12.

[0065] like Figures 3-5 As shown, the gas positive and negative pressure control subsystem 21 is used to provide a positive, negative, or normal pressure biological sample development environment for biological culture. The gas positive and negative pressure control subsystem 21 includes a switching valve 212 and a positive and negative pressure generator 211. The switching valve 212 is sequentially equipped with a mixed gas inlet 212a, a normal pressure inlet 212b, a pressure regulating input inlet 212c, and an output inlet 212d. The mixed gas inlet 212a is connected to the output terminal of the concentration control subsystem 23, and the output terminal of the concentration control subsystem 23 is connected to the positive and negative pressure generator 211 through a mixed gas inlet pipe 211a; the normal pressure... The ventilation is connected to the air; the pressure regulating input vent 212c is connected to the output end of the positive and negative pressure generator 211, and the output vent 212d is connected to the input end of the humidity control subsystem 24; the return air pipe 117 is connected to the input end of the positive and negative pressure generator 211 through the return air vent pipe 211b; the switching valve 212 and the positive and negative pressure generator 211 are both electrically connected to the control host 3, and are used to regulate the opening and closing of the corresponding vent of the switching valve 212 and the working power of the positive and negative pressure generator 211.

[0066] The gas positive and negative pressure subsystem keeps the mixed gas in the gas path in normal pressure, positive pressure, and negative pressure states according to the set pressure parameters.

[0067] The gas positive and negative pressure control process is as follows:

[0068] Atmospheric pressure process:

[0069] Switching valve 212 opens the mixed gas inlet 212a, the atmospheric pressure inlet 212b, the pressure regulating input inlet 212c, and the output inlet 212d. Positive and negative pressure generator 211 opens the return gas inlet 211b and closes the mixed gas inlet 211a. Positive and negative pressure generator 211 connects the return gas inlet 211b with the pressure regulating input inlet 212c of switching valve 212. The mixed gas passing through the rotary culture system 1 flows out into the air through the atmospheric pressure inlet 212b of switching valve 212. The gas pressure of the mixed gas in the rotary culture system 1 is at atmospheric pressure.

[0070] Positive pressure process:

[0071] Switching valve 212 closes the mixed gas inlet 212a and the atmospheric pressure inlet 212b, and opens the pressure regulating input inlet 212c and the output inlet 212d. Positive and negative pressure generator 211 closes the return gas inlet 211b and opens the mixed gas inlet 211a. Positive and negative pressure generator 211 performs gas pressurization operation on the rotary culture system 1.

[0072] Negative pressure state process:

[0073] Switching valve 212 opens mixed gas inlet 212a, atmospheric pressure inlet 212b, pressure regulating input inlet 212c and output inlet 212d. Positive and negative pressure generator 211 opens return gas inlet 211b and closes mixed gas inlet 211a. Positive and negative pressure generator 211 performs negative gas operation on rotating culture system 1.

[0074] The temperature control subsystem 22 includes a constant temperature chamber. The rotary culture system 1 is installed inside the constant temperature chamber to provide a stable temperature development environment. A chamber temperature control unit 222 is installed inside the constant temperature chamber. A door temperature control unit 221 is installed on the door of the constant temperature chamber. A humidifier bottle temperature control unit 223 is installed on the side of the constant temperature chamber to provide a stable temperature environment for the humidifier bottle 241. The chamber temperature control unit 222, the door temperature control unit 221, and the humidifier bottle temperature control unit 223 all include an electric heating plate 22a and a temperature sensor. The heating plates 22a are distributed throughout the constant temperature chamber. The electric heating plates 22a and the temperature sensors are electrically connected to the control host 3.

[0075] The concentration control subsystem 23 is used to provide a stable gas concentration development environment for biological culture. The concentration control subsystem 23 includes an air intake control component 231. The input end of the air intake control component 231 is provided with an air inlet 231a, an oxygen inlet 231b, a carbon dioxide inlet 231c, and a nitrogen inlet 231d. The output end of the air intake control component 231 is connected to a fan pump 233, which provides circulation power for the gas flow in the gas path. A gas concentration detection component 232 is provided between the output end of the air intake control component 231 and the fan pump 233 to detect the corresponding gas concentration in the output gas.

[0076] The intake control component 231, the fan pump 233, and the gas concentration detection component 232 are all electrically connected to the control host 3.

[0077] The concentration control subsystem 23 mixes corresponding gases according to the set concentration parameters to form a mixed gas. The gas concentration detection component 232 detects the gas concentration of the corresponding gas in the gas path, compares the detected gas concentration with the set gas concentration parameters, and feeds the result back to the control host 3. The control host 3 adjusts the intake control component 231 accordingly to supplement the corresponding gas until the gas concentration of the corresponding gas reaches the set concentration. It can provide high oxygen concentration development environment, low oxygen concentration development environment, high carbon dioxide concentration development environment, and low carbon dioxide concentration development environment.

[0078] The oxygen concentration adjustment procedure is as follows:

[0079] Open the air vent 231a and nitrogen vent 231d, and close the oxygen vent 231b. Reduce the oxygen concentration by continuously supplying nitrogen to expel oxygen from the gas path in the concentration control subsystem 23. The oxygen concentration is less than 210,000 ppm (the oxygen concentration in the air).

[0080] Close the air vent 231a and nitrogen vent 231d, and open the oxygen vent 231b to continuously supply oxygen to increase the oxygen concentration in the gas path of the concentration control subsystem 23 to a level greater than 210,000 ppm.

[0081] Close oxygen vent 231b and nitrogen vent 231d, and open air vent 231a to continuously supply air to increase the oxygen concentration in the gas path of the concentration control subsystem to an oxygen concentration of 210,000 ppm.

[0082] The carbon dioxide adjustment procedure is as follows:

[0083] By closing the carbon dioxide vent 231c and opening the air vent 231a or nitrogen vent 231d, the carbon dioxide concentration is reduced by continuously introducing air or nitrogen into the gas path of the concentration control subsystem 23.

[0084] Open the carbon dioxide vent 231c to continuously introduce carbon dioxide into the gas path of the concentration control subsystem 23, thereby increasing the carbon dioxide concentration.

[0085] The humidity control subsystem 24 provides a set humidity environment for biological culture. The humidity control subsystem 24 includes a humidifier bottle 241 and a humidity detection component 242 mounted on the return gas pipe 117. The humidity detection component 242 detects the humidity of the mixed gas flowing out after passing through the spherical-bottom culture flask 12. The input end of the humidifier bottle 241 is connected to the output vent 212d, and the output end of the humidifier bottle 241 is connected to the rotary joint 114. Both the humidifier bottle 241 and the humidity detection component 242 are electrically connected to the control host 3. After being humidified by the humidifier bottle 241, the mixed gas enters the spherical-bottom culture flask 12 in the rotary culture system 1, and the humidity is detected by the humidity detection component 242 on the return gas pipe 117. The operating power of the humidifier bottle 241 is adjusted according to the detected humidity data and the set humidity.

[0086] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and not to limit it. Although the utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solution of this utility model, and these modifications or equivalent substitutions cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of this utility model.

Claims

1. A positive and negative pressure rotating biological culture device, comprising a control host and an environmental control system, characterized in that: It also includes a rotary culture system set within the environmental control system. The rotary culture system includes a rotary mechanism and at least one spherical bottom culture bottle. The spherical bottom culture bottle is set on the rotary mechanism, so that the spherical bottom culture bottle rotates under the drive of the rotary mechanism. The spherical bottom culture flask includes a spherical culture section and a cylindrical connecting section. The spherical culture section is used to hold biological samples. One end of the cylindrical connecting section is connected to the spherical culture section, and the other end of the cylindrical connecting section is provided with a detachable connecting section. When biological samples and nutrient solutions are placed in the spherical bottom culture flask, the liquid level of the biological samples and nutrient solutions is lower than the lowest point of the cylindrical connecting section when the spherical bottom culture flask is installed horizontally. The rotating mechanism includes a rotary drive motor for providing rotational power. The rotary drive motor is connected to a gas distribution plate via a rotary joint. The rotary joint is connected to an environmental control system, enabling the rotary drive motor to simultaneously deliver gas, regulate pressure, and adjust temperature and humidity while driving the gas distribution plate. Several gas distribution blocks are connected to the gas distribution plate, and each gas distribution block is equipped with a connecting seat. The connecting seat is connected to a detachable connecting section. Each gas distribution block is equipped with an inlet pipe and a return pipe for gas exchange within the spherical bottom culture flask. The tail end of the inlet pipe is positioned opposite to the spherical culture section, and the tail end of the return pipe is located within the cylindrical connecting section. The rotary drive motor is connected to the main control unit for regulating the rotational speed of the gas distribution plate.

2. The positive and negative pressure rotating biological culture device according to claim 1, characterized in that: The environmental control system includes a gas positive and negative pressure control subsystem, a temperature control subsystem, a concentration control subsystem, and a humidity control subsystem; The concentration control subsystem is connected to the positive and negative pressure control subsystem, which in turn is connected to the humidity control subsystem. The humidity control subsystem is also connected to the rotary joint. The rotary joint is connected to the positive and negative pressure control subsystem. The gas positive and negative pressure control subsystem, temperature control subsystem, concentration control subsystem, and humidity control subsystem are all electrically connected to the main control unit and are used to regulate the culture environment of the spherical bottom culture flask.

3. The positive and negative pressure rotating biological culture device according to claim 2, characterized in that: The gas positive and negative pressure control subsystem is used to provide a positive pressure, negative pressure, or normal pressure biological sample development environment for biological culture. The gas positive and negative pressure control subsystem includes a switching valve and a positive and negative pressure generator. The switching valve is sequentially equipped with a mixed gas inlet, an atmospheric pressure inlet, a pressure regulating input inlet, and an output inlet. The mixed gas inlet is connected to the output of the concentration control subsystem, which in turn is connected to the positive and negative pressure generator via a mixed gas inlet pipeline. The atmospheric pressure inlet is open to air. The pressure regulating input inlet is connected to the output of the positive and negative pressure generator, and the output inlet is connected to the input of the humidity control subsystem. The return gas pipe is connected to the input of the positive and negative pressure generator via a return gas inlet pipeline. Both the switching valve and the positive and negative pressure generator are electrically connected to the control host.

4. The positive and negative pressure rotating biological culture device according to claim 3, characterized in that: The temperature control subsystem includes a constant temperature chamber, a rotary culture system located inside the constant temperature chamber to provide a stable temperature development environment, a chamber temperature control unit inside the constant temperature chamber, a door temperature control unit on the door of the constant temperature chamber, and a humidifier bottle temperature control unit on the side of the constant temperature chamber to provide a stable temperature environment for the humidifier bottle. The chamber temperature control unit, door temperature control unit, and humidifier bottle temperature control unit all include an electric heating plate and a temperature sensor. The heating plate inside the constant temperature chamber is located on the inner wall of the constant temperature chamber, and both the electric heating plate and the temperature sensor are electrically connected to the control host.

5. The positive and negative pressure rotating biological culture device according to claim 4, characterized in that: The concentration control subsystem is used to provide a stable gas concentration development environment for biological culture; The concentration control subsystem includes an air intake control component. The input end of the air intake control component is equipped with an air inlet, an oxygen inlet, a carbon dioxide inlet, and a nitrogen inlet. The output end of the air intake control component is connected to a fan pump, which provides circulation power for the gas flow in the gas path. A gas concentration detection component is set between the output end of the air intake control component and the fan pump to detect the concentration of the corresponding gas in the output gas. The intake control unit, fan pump, and gas concentration detection unit are all electrically connected to the control host.

6. The positive and negative pressure rotating biological culture device according to claim 5, characterized in that: The concentration control subsystem mixes the corresponding gases according to the set concentration parameters to form a mixed gas; The gas concentration detection component detects the gas concentration of the corresponding gas in the gas path, compares the detected gas concentration with the set gas concentration parameter, and feeds it back to the control host. The control host adjusts the intake control component accordingly to supplement the corresponding gas until the gas concentration of the corresponding gas reaches the set concentration. The oxygen concentration adjustment procedure is as follows: Open the air vent and nitrogen vent, and close the oxygen vent. Reduce the oxygen concentration by continuously supplying nitrogen to expel oxygen from the gas path in the concentration control subsystem. The oxygen concentration should be less than 210,000 ppm. Close the air vent and nitrogen vent, and open the oxygen vent. By continuously supplying oxygen, the oxygen concentration in the gas circuit of the concentration control subsystem is increased to a level greater than 210,000 ppm. Close the oxygen and nitrogen vents and open the air vent to continuously supply air to increase the oxygen concentration in the gas path of the concentration control subsystem. The oxygen concentration is equal to 210,000 ppm. The carbon dioxide adjustment procedure is as follows: Close the carbon dioxide vent and open the air or nitrogen vent to continuously introduce air or nitrogen into the gas path of the concentration control subsystem to reduce the carbon dioxide concentration. Open the carbon dioxide vent to continuously introduce carbon dioxide into the gas path of the concentration control subsystem, thereby increasing the carbon dioxide concentration.

7. The positive and negative pressure rotating biological culture device according to claim 6, characterized in that: The humidity control subsystem is used to provide a set humidity environment for biological culture; the humidity control subsystem includes a humidifier bottle and a humidity detection component installed on the return gas pipe. The humidity detection component is used to detect the humidity of the mixed gas flowing out after passing through the spherical bottom culture bottle; the input end of the humidifier bottle is connected to the output vent, and the output end of the humidifier bottle is connected to a rotary joint. Both the humidifier bottle and the humidity detection component are electrically connected to the control unit; The mixed gas is humidified by the humidifier bottle and then enters the spherical bottom culture bottle in the rotating culture system. The humidity is detected by the humidity detection component on the return gas pipe, and the working power of the humidifier bottle is adjusted according to the detected humidity data and the set humidity.

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