Cell wall crushing system for bacterial sludge

Abstract

The utility model discloses a bacterial sludge cell wall breaking system which comprises a homogenizer body, the homogenizer body comprises a machine body and a feeding cylinder arranged at one end of the machine body, the bottom end of the feeding cylinder is fixedly provided with a connecting pipe communicated with the machine body, and the top end of the feeding cylinder is provided with a sealing cylinder cover. A feeding pipe for feeding is fixedly mounted at the top end of the sealing cylinder cover, and a scraping assembly for scraping the inner wall of the feeding cylinder is arranged at the top end of the sealing cylinder cover. The scraping assembly comprises a rotating shaft rotationally connected to the interior of the feeding cylinder, an adjusting cabinet fixedly installed at the top end of the sealing cylinder cover and a first rotating motor fixedly installed at the top end of the adjusting cabinet, the rotating shaft penetrates through the sealing cylinder cover and is rotationally connected with the adjusting cabinet and the first rotating motor, and a connecting sleeve is slidably connected to the periphery of the rotating shaft. According to the feeding device, a bacterial solution adhered to the inner wall of the feeding cylinder can be scraped through rotation of the scraping plate, so that the bacterial solution adhered to the inner wall of the feeding cylinder is reduced, and bacterial breeding is reduced.

Description

Technical Field

[0001] This utility model relates to the field of bacterial mud cell wall disruption technology, specifically a bacterial mud cell wall disruption system. Background Technology

[0002] Specific cells are fermented in a fermenter to form a cell culture medium, which is then centrifuged at high speed in a disc centrifuge to obtain bacterial sludge. The bacterial sludge is resuspended in a buffer solution and then broken up (cell wall is broken) by a homogenizer. The breakage rate is observed under a microscope. The homogenizer is a piece of equipment widely used in food processing, pharmaceuticals, chemicals and other industries. Its main function is to use high-speed rotating blades or needles to shear, impact and mix materials, thereby achieving material homogenization.

[0003] Homogenizers effectively break down cell walls and release cell contents such as proteins, enzymes, and DNA through high-speed rotating blades or needles. When a bacterial solution is fed from the feed cylinder into the homogenizer, the solution itself has a certain viscosity. This viscosity causes some of the solution to adhere to the inner wall of the feed cylinder. If not treated in time, this can lead to bacterial growth and affect the subsequent processing of the cell solution. Utility Model Content

[0004] The purpose of this invention is to provide a cell wall breaking system for bacterial sludge, which addresses the problem that when bacterial solution is transported from the feed cylinder of a homogenizer to the inside of the homogenizer, some of the solution adheres to the inner wall of the feed cylinder due to its viscosity. If not treated in time, this can lead to bacterial growth and affect the subsequent processing of the cell solution.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell wall breaking system for bacterial sludge, comprising a homogenizer body, the homogenizer body including a machine body and a feed cylinder disposed at one end of the machine body, a connecting pipe fixedly installed at the bottom end of the feed cylinder and communicating with the machine body, a sealing cylinder cover disposed at the top end of the feed cylinder, a feed pipe for feeding is fixedly installed at the top end of the sealing cylinder cover, and a scraping assembly for scraping the inner wall of the feed cylinder is disposed at the top end of the sealing cylinder cover, the scraping assembly including a rotating shaft rotatably connected to the inside of the feed cylinder, an adjusting cabinet fixedly installed at the top end of the sealing cylinder cover, and a rotating motor fixedly installed at the top end of the adjusting cabinet, the rotating shaft communicating rotatably with the sealing cylinder cover, the adjusting cabinet, and the rotating motor, a connecting sleeve slidably connected to the outer circumference of the rotating shaft, and a scraper for scraping the bacterial liquid adhering to the inner wall of the feed cylinder uniformly and fixedly installed around the outer circumference of the connecting sleeve.

[0006] As a further embodiment of this utility model: the connecting sleeve passes through the sealing sleeve cover and the regulating cabinet, and a matching sleeve is fixedly installed on the outer periphery. A second rotating motor is fixedly installed inside the regulating cabinet. One end of the second rotating motor is rotatably connected to a rotating gear, and the rotating gear rotates in cooperation with the matching sleeve.

[0007] As a further embodiment of this utility model: a sliding groove is provided on the outer periphery of the rotating shaft for the connecting sleeve to slide and adjust, and the sliding groove is symmetrically provided on the outer periphery of the rotating shaft. A sliding rod that is symmetrically adapted to slide the sliding groove is fixed on the inner side of the connecting sleeve.

[0008] As a further embodiment of this utility model: a sealing ring is fixedly provided at the top of the feed cylinder to form a sealing connection with the sealing cylinder cover, and a sealing groove adapted to the sealing ring is provided at the bottom of the sealing cylinder cover.

[0009] As a further improvement of this utility model, the sealing cylinder cover is symmetrically provided with handles at both ends for workers to move the sealing cylinder cover.

[0010] As a further embodiment of this utility model: a support rod is fixedly installed at the bottom of the machine body for support, and the support rod is symmetrically fixedly installed at the bottom of the machine body.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. This utility model uses the rotation of the scraper to scrape away the bacterial solution adhering to the inner wall of the feed cylinder, thereby reducing the bacterial solution adhering to the inner wall of the feed cylinder and reducing bacterial growth.

[0013] 2. By rotating the motor, rotating the gears, and using the sleeve, the scraper can be moved up and down inside the feed cylinder, reducing the adhesion of bacterial solution in the dead corners inside the feed cylinder. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0015] Figure 2 This is a side view of the feed cylinder of this utility model;

[0016] Figure 3 This is a cross-sectional structural diagram of the feed cylinder and regulating cabinet of this utility model;

[0017] Figure 4 This is a utility model Figure 3 A magnified schematic diagram of the structure at point A in the middle.

[0018] In the diagram: 1. Homogenizer body; 11. Machine body; 12. Support base rod; 13. Feed cylinder; 14. Sealing cylinder cover; 15. Connecting pipe; 16. Feed pipe; 17. Sealing ring; 18. Sealing groove; 19. Handle; 2. Scraper assembly; 21. Adjusting cabinet; 22. Rotating shaft; 23. Connecting sleeve; 24. Scraper; 25. Rotating motor one; 26. Rotating motor two; 27. Rotating gear; 28. Matching sleeve; 29. ​​Slide groove; 210. Slide rod. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In the description of this utility model, it should be noted that unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances. The embodiments of this utility model will be described below based on its overall structure.

[0021] Reference Figures 1 to 4In this embodiment of the invention, a cell wall breaking system for bacterial sludge includes a homogenizer body 1. The homogenizer body 1 includes a body 11 and a feed cylinder 13 disposed at one end of the body 11. A connecting pipe 15, which is connected to the body 11, is fixedly installed at the bottom of the feed cylinder 13. A sealing cylinder cover 14 is provided at the top of the feed cylinder 13. A feed pipe 16 for feeding is fixedly installed at the top of the sealing cylinder cover 14. A scraping component 2 for scraping the inner wall of the feed cylinder 13 is provided at the top of the sealing cylinder cover 14. The scraping assembly 2 includes a rotating shaft 22 rotatably connected inside the feed cylinder 13, an adjusting cabinet 21 fixedly installed on the top of the sealing cylinder cover 14, and a rotating motor 25 fixedly installed on the top of the adjusting cabinet 21. The rotating shaft 22 passes through the sealing cylinder cover 14 and is rotatably connected to the adjusting cabinet 21 and the rotating motor 25. A connecting sleeve 23 is slidably connected to the outer periphery of the rotating shaft 22, and a scraper 24 for scraping the bacterial liquid adhering to the inner wall of the feed cylinder 13 is uniformly fixedly installed around the outer periphery of the connecting sleeve 23.

[0022] Through the implementation of this method: the start of the rotating motor 25 will drive the rotating shaft 22 to rotate, which in turn drives the connecting sleeve 23 to rotate. As the connecting sleeve 23 rotates, the outer scraper 24 will also rotate. The rotation of the scraper 24 will scrape the bacterial solution adhering to the inner wall of the feed cylinder 13, reducing the bacterial solution adhering to the inner wall of the feed cylinder 13 and reducing bacterial growth.

[0023] Reference Figures 1 to 4 The connecting sleeve 23 passes through the sealing sleeve cover 14 and the regulating cabinet 21. A matching sleeve 28 is fixedly installed on the outer periphery. A rotating motor 26 is fixedly installed inside the regulating cabinet 21. One end of the rotating motor 26 is rotatably connected to a rotating gear 27, and the rotating gear 27 rotates in cooperation with the matching sleeve 28. A sliding groove 29 is opened on the outer periphery of the rotating shaft 22 for the connecting sleeve 23 to slide and adjust. The sliding groove 29 is symmetrically opened on the outer periphery of the rotating shaft 22. A sliding rod 210 that is symmetrically fixed on the inner side of the connecting sleeve 23 and is adapted to slide the sliding groove 29.

[0024] By implementing this method: by starting the rotating motor 26, the rotating motor 26 will drive the rotating gear 27 to rotate. When the rotating gear 27 rotates, it will cooperate with the mating sleeve 28 set on the outer periphery of the connecting sleeve 23. At this time, the mating sleeve 28 will rise and fall through the sliding groove 29 and the sliding rod 210, thereby driving the scraper 24 to rise and fall inside the feed cylinder 13, reducing the residue of bacterial solution in the dead corner of the inner wall of the feed cylinder 13.

[0025] Reference Figures 1 to 4The top of the feed cylinder 13 is fixed with a sealing ring 17 that is sealed to the sealing cylinder cover 14, and the bottom of the sealing cylinder cover 14 is provided with a sealing groove 18 that is compatible with the sealing ring 17. The two ends of the sealing cylinder cover 14 are symmetrically fixed with a moving handle 19 for workers to move the sealing cylinder cover 14.

[0026] Through this method, the sealing ring 17 is made of rubber material, which has excellent elasticity, enabling the rubber seal to adapt to different sealing surface shapes and size changes, effectively filling the sealing gap.

[0027] Reference Figures 1 to 4 A support rod 12 is fixedly installed at the bottom of the body 11 for support, and the support rod 12 is symmetrically fixed at the bottom of the body 11.

[0028] The working principle of this utility model is as follows: The bacterial sludge obtained by centrifugation in a disc centrifuge is resuspended in a buffer solution. When it is necessary to break the cell walls, the operator first places the sealing cap 14 at the top of the feed cylinder 13 by moving the handle 19. Then, the bacterial solution is placed into the feed cylinder 13 through the feed pipe 16 at the top of the sealing cap 14. After that, the bacterial solution is transported to the machine body 11 through the connecting pipe 15 to break the cell walls of the bacterial solution. When the bacterial solution passes through the feed cylinder 13, due to its own viscosity, some of it will adhere to the wall surface. At this time, the operator only needs to start the rotating motor 25. The start of the rotating motor 25 will drive the rotating shaft 22 to rotate, which in turn drives the connecting sleeve 23 to rotate. As the connecting sleeve 23 rotates, it will drive the outer scraper 24 to rotate as well. The system scrapes away the bacterial solution adhering to the inner wall of the feed cylinder 13, reducing bacterial growth. Simultaneously, by activating the second rotating motor 26, the rotating gear 27 rotates. When the rotating gear 27 rotates, it engages with the connecting sleeve 28 located on the outer periphery of the connecting sleeve 23. The outer periphery of the connecting sleeve 23 is an annular structure that rotates in conjunction with the rotating gear 27. At this time, the connecting sleeve 28 rises and falls via the sliding groove 29 and the sliding rod 210, thereby causing the scraper 24 to rise and fall inside the feed cylinder 13, reducing the residue of bacterial solution in the dead corners of the feed cylinder 13. Through the rotation of the second rotating motor 26, the rotating gear 27, and the connecting sleeve 28, the scraper 24 can rise and fall inside the feed cylinder 13, reducing the adhesion of bacterial solution in the dead corners of the feed cylinder 13.

[0029] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cell wall disruption system for fungal sludge, characterized in that, The homogenizer includes a homogenizer body (1), which includes a machine body (11) and a feed cylinder (13) disposed at one end of the machine body (11). A connecting pipe (15) that is connected to the machine body (11) is fixedly installed at the bottom end of the feed cylinder (13). A sealing cylinder cover (14) is provided at the top end of the feed cylinder (13). A feed pipe (16) for feeding is fixedly installed at the top end of the sealing cylinder cover (14). A scraping assembly (2) for scraping the inner wall of the feed cylinder (13) is provided at the top end of the sealing cylinder cover (14). The scraping assembly (2) includes... The device includes a rotating shaft (22) rotatably connected inside the feed cylinder (13), an adjusting cabinet (21) fixedly installed on the top of the sealing cylinder cover (14), and a rotating motor (25) fixedly installed on the top of the adjusting cabinet (21). The rotating shaft (22) passes through the sealing cylinder cover (14) and is rotatably connected to the adjusting cabinet (21) and the rotating motor (25). A connecting sleeve (23) is slidably connected to the outer circumference of the rotating shaft (22), and a scraper (24) is uniformly and fixedly installed around the outer circumference of the connecting sleeve (23) to scrape the bacterial liquid adhering to the inner wall of the feed cylinder (13).

2. The cell wall disruption system for fungal sludge according to claim 1, characterized in that, The connecting sleeve (23) passes through the sealing sleeve cover (14) and the regulating cabinet (21) and a matching sleeve (28) is fixedly installed on its outer periphery. A rotating motor (26) is fixedly installed inside the regulating cabinet (21). One end of the rotating motor (26) is rotatably connected to a rotating gear (27), and the rotating gear (27) rotates in cooperation with the matching sleeve (28).

3. The cell wall disruption system for fungal sludge according to claim 1, characterized in that, The outer periphery of the rotating shaft (22) is provided with a sliding groove (29) for the connecting sleeve (23) to slide and adjust. The sliding groove (29) is symmetrically provided on the outer periphery of the rotating shaft (22). The inner side of the connecting sleeve (23) is symmetrically provided with a sliding rod (210) that is adapted to slide with the sliding groove (29).

4. The cell wall disruption system for fungal sludge according to claim 1, characterized in that, The top end of the feed cylinder (13) is fixed with a sealing ring (17) that is sealed to the sealing cylinder cover (14), and the bottom end of the sealing cylinder cover (14) is provided with a sealing groove (18) that is compatible with the sealing ring (17).

5. The cell wall disruption system for fungal sludge according to claim 1, characterized in that, The sealing cylinder cover (14) is symmetrically fixed at both ends with handles (19) for workers to move the sealing cylinder cover (14).

6. The cell wall disruption system for fungal sludge according to claim 1, characterized in that, The bottom of the body (11) is fixedly installed with a support rod (12) for support, and the support rod (12) is symmetrically fixedly installed at the bottom of the body (11).

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