Protein immunoblotting instrument

Abstract

The utility model relates to the technical field of protein immunoblotting instruments, in particular to a protein immunoblotting instrument. According to the technical scheme, the device comprises a shell and a reagent groove used for loading a reagent, a suspension arm used for stretching into the reagent groove to carry out an experiment is installed on the shell, and a shaking mechanism used for shaking the reagent groove is arranged in the shell. According to the utility model, the two rails are arranged at different placement positions, so that when a protein sample is loaded in the reagent tank, pressure is formed on the accommodating shell, the guide rod below the accommodating shell is in contact with the moving rails, the guide rod is in close contact with the surfaces of the rails, and when the guide rod moves along the rails, the protein sample is separated from the accommodating shell. The guide rod can push the accommodating shell to do reciprocating inclined motion in the built-in groove I, so that a protein sample in the reagent tank can be properly shaken, the progress of a western blot experiment can be accelerated, and the accuracy of an experiment result can be improved.

Description

Technical Field

[0001] This utility model relates to the field of protein immunoblotting technology, and in particular to a protein immunoblotting instrument. Background Technology

[0002] A protein immunoblotting instrument is an automated device used for protein immunoblotting experiments, a technique widely used in biological and medical research to detect the expression of specific proteins in cells or tissues. The instrument uses specific antibodies to stain gel electrophoresis-treated cell or biological tissue samples, analyzing the location and depth of the staining to obtain the expression status of specific proteins. The experimental procedure generally involves using a crane to mix the extracted protein sample with the gel in a reagent tank, then transferring the protein to the corresponding reagent tank using the crane. Depending on the experimental method, different antigens are added sequentially via the crane, and chemical methods are used to make the target protein develop color in the mixture. Currently available protein immunoblotting instruments include fully automated immunoblotting instruments and traditional immunoblotting instruments.

[0003] Because there are many models of fully automated immunoblotting instruments with varying costs, most laboratories use small fully automated immunoblotting instruments that possess the basic functions of large fully automated immunoblotting instruments at a lower cost, thus attracting a wider user base. However, these instruments are relatively simple in structure and can only operate according to pre-entered programs. In protein immunoblotting experiments, steps such as gel preparation, transfer, and antibody application require shaking to improve the results. Small fully automated immunoblotting instruments lack this shaking function, requiring manual shaking or omitting this step, which can easily introduce errors into the protein immunoblotting results and hinder precise protein immunoblotting experiments. Therefore, this application proposes a protein immunoblotting instrument. Utility Model Content

[0004] The purpose of this invention is to address the problem in the prior art that small fully automated immunoblotting instruments cannot accelerate protein replication in culture, and to propose a protein immunoblotting instrument.

[0005] The technical solution of this utility model is as follows: a protein immunoblotting instrument, including a housing and a reagent tank for loading reagents, wherein a lifting arm for extending into the reagent tank to conduct experiments is installed on the housing, and a shaking mechanism for shaking the reagent tank is provided inside the housing.

[0006] The shaking mechanism includes a tray mounted on top of the housing, with multiple slots continuously opened inside the tray. Each slot is slidably connected to a receiving shell for accommodating reagent tanks. Two guide rods are symmetrically fixedly connected to the lower end of the receiving shell. A motor is fixedly connected inside the housing. One end of the motor's output shaft is fixedly connected to a gear, and an internal gear meshes with one side of the gear. A disc is fixedly connected to the upper end of the internal gear. The disc is rotatably connected to the housing, and two annular tracks that press against the guide rods are fixedly connected to the upper end of the disc.

[0007] Optionally, the track includes an ascending section and a descending section, and the track is a closed-loop wave shape, with the ascending sections on the two tracks being staggered.

[0008] Optionally, each slot has a built-in groove 1, and two sets of V-shaped elastic plates are symmetrically fixedly connected in the built-in groove 1 and respectively fixedly connected to both ends of the receiving shell. The tray has multiple built-in grooves 2, and a slider that slides in the built-in groove 2 is fixedly connected to one side of the receiving shell. A rotating rod is rotatably connected in the slider, and two round rods are symmetrically slidably connected in the slider.

[0009] Optionally, the built-in groove 2 is provided with a circular groove for sliding connection with the circular rod.

[0010] Optionally, one end of the rotating rod passes through the slider, the rotating rod is elliptical, and a circular block is fixedly connected to one end of the rotating rod.

[0011] Optionally, both ends of the two circular rods are tapered, the circular rods are made of magnets, the slider is made of plastic, the rotating rod is made of iron, and the circular rods and the rotating rod are magnetically connected.

[0012] Optionally, the length of the slot is less than the length of the receiving shell.

[0013] Compared with the prior art, this application includes at least one of the following beneficial technical effects:

[0014] This invention features two tracks positioned at different locations. When a protein sample is loaded into the reagent tank, pressure is applied to the container shell, causing the guide rod below the container shell to contact the moving track. At this point, the guide rod makes close contact with the track surface. As the guide rod moves along the track, it pushes the container shell to reciprocate and tilt within the built-in groove, allowing the protein sample in the reagent tank to be properly shaken. This helps to accelerate the protein immunoblotting experiment and improve the accuracy of the experimental results.

[0015] Furthermore, by setting the slider to slide in the second built-in groove, the slider and the housing can move synchronously. The experimenter can rotate the circular block to control the movement of the slider in the second built-in groove. This allows the corresponding reagent tank to be adjusted in an orderly manner to stop shaking according to the progress of the protein immunoblotting experiment in different reagent tanks. This is beneficial for the experimenter to conduct repeated experiments in the laboratory and compare the experimental results, thereby reducing errors in the experimental process. Attached Figure Description

[0016] Figure 1 A schematic front view of the overall structure of a protein immunoblotting instrument is provided.

[0017] Figure 2 This is a schematic diagram of the internal groove structure.

[0018] Figure 3 This is a schematic diagram of the side section structure of the slot;

[0019] Figure 4 This is a schematic diagram of the rotating rod structure.

[0020] Reference numerals: 1. Shell; 101. Reagent tank; 102. Hanging arm; 2. Shaking mechanism; 201. Tray; 202. Groove; 203. Receiving shell; 204. Guide rod; 205. Motor; 206. Gear one; 207. Internal gear; 208. Disc; 209. Track; 210. Lifting section; 211. Lowering section; 212. Internal groove one; 213. V-shaped elastic sheet; 214. Internal groove two; 215. Slider; 216. Rotating rod; 217. Round rod; 218. Round groove; 219. Round block. Detailed Implementation

[0021] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. Example

[0022] like Figures 1 to 4 As shown, the present invention proposes a protein immunoblotting instrument, including a housing 1 and a reagent tank 101 for loading reagents. A lifting arm 102 for inserting into the reagent tank 101 to conduct experiments is installed on the housing 1. A shaking mechanism 2 for shaking the reagent tank 101 is provided inside the housing 1. Different antigens and reagents can be loaded into multiple reagent tanks 101, and labels are added to the reagent tanks 101 for human differentiation. When the housing 1 starts working, it drives the lifting arm 102 to put different reagents into different reagent tanks 101. At the same time, the fully automated process realizes the protein immunoblotting experiment.

[0023] The shaking mechanism 2 includes a tray 201 mounted above the housing 1. Multiple slots 202 are continuously formed within the tray 201. Each slot 202 contains a receiving shell 203 slidably connected to accommodate a reagent tank 101. The length of the slot 202 is shorter than the length of the receiving shell 203. When the receiving shell 203 is shaken within the slot 202, the larger slot 202 facilitates lateral swinging motion. The reagent tank 101 is placed in the receiving shell 203, with different reagent tanks 101 placed into their corresponding receiving shells 203, which are then placed into the slots 202. The different slots 202 are all the same size, and only the internal space of the receiving shell 203 is different, while the external dimensions of the receiving shell 203 are all the same. Two guide rods 204 are symmetrically fixedly connected to the lower end of the receiving shell 203. The guide rods 204 can contact and press with the annular track 209, so that the two guide rods 204 push one side of the receiving shell 203 to move in turn, thereby creating a shaking effect on the reagent tank 101. A motor 205 is fixedly connected inside the shell 1. A gear 206 is fixedly connected to one end of the output shaft of the motor 205. An internal gear 207 meshes with one side of the gear 206. A disc 208 is fixedly connected to the upper end of the container. The disc 208 is rotatably connected to the housing 1. Two annular tracks 209 are fixedly connected to the upper end of the disc 208, which are in contact with the guide rods 204. The tracks 209 include an ascending section 210 and a descending section 211, and are closed-loop wavy. When both guide rods 204 simultaneously contact their corresponding tracks 209, the two guide rods 204 are located in the ascending section 210 or descending section 211 respectively, maintaining the reagent tank 101 in an inclined position within the housing 203. This facilitates faster and more uniform mixing of the reagents within the reagent tank 101. The lifting section 210 on the 9 is placed in a staggered manner. When the motor 205 is working, it will drive the gear 206 to rotate and mesh with the internal gear 207. At this time, the internal gear 207 and the disk 208 will perform circular motion, causing the two tracks 209 above the disk 208 to move. Since the lifting sections 210 of the two tracks 209 are placed in a staggered manner, when the lifting section 210 of the track 209 contacts the guide rod 204, the guide rod 204 will move upward on the side supporting the housing 203, and at the same time, under the action of its own gravity, it will move downward along the lowering section 211, thereby creating a shaking effect on the reagent in the reagent tank 101.

[0024] In this implementation, such as Figure 3As shown, each slot 202 has a built-in groove 212, the width of which is greater than the width of the slot 202. The opening end of the receiving shell 203 has an outwardly extending portion, which can be locked in the built-in groove 212 and will not slip out. Two sets of V-shaped elastic sheets 213 are symmetrically fixedly connected in the built-in groove 212 and respectively fixedly connected to both ends of the receiving shell 203. These sheets have a certain degree of deformation. When a certain amount of reagent is added to the reagent tank 101, a certain amount of gravity is applied to the reagent tank 101 and the receiving shell 203, causing the receiving shell 203 to compress the V-shaped elastic sheets 213. The internal groove 212 moves downward, allowing the two guide rods 204 to contact the track 209. The tray 201 has multiple internal grooves 214. When the receiving shell 203 moves downward within the internal groove 212, it drives the slider 215 to move synchronously within the internal grooves 214. A circular groove 218 is fixedly connected within the internal groove 214 for sliding connection with the round rod 217. The user can insert the round rod 217 into the circular groove 218, thereby restricting the movement of the slider 215 within the internal groove 214 and simultaneously restricting the movement of the receiving shell 203 within the internal groove 212, thus allowing the independent test... The guide rod 204 below the reagent tank 101 contacts the track 209, which facilitates the user to adjust the experimental reaction in the reagent tank 101 in a timely manner according to the development of the experiment. A slider 215 is fixedly connected to one side of the housing 203, sliding within the internal groove 214. A rotating rod 216 is rotatably connected inside the slider 215, with one end of the rotating rod 216 passing through the slider 215. The rotating rod 216 is elliptical in shape, and a circular block 219 is fixedly connected to one end of the rotating rod 216. Two circular rods 217 are symmetrically arranged inside the slider 215, with both ends of the two circular rods 217 being conical. The circular rods 217 are magnets, and the slider 215 is made of plastic. Rod 216 is made of iron. The round rod 217 is magnetically connected to the rotating rod 216. The user can rotate the round block 219 to make the rotating rod 216 rotate. Since the rotating rod 216 is elliptical and the rotating rod 216 is magnetically connected to the round rod 217, the rotating rod 216 can squeeze the round rod 217 to move linearly within the slider 215, and can squeeze the round rod 217 into the round groove 218, thereby fixing the receiving shell 203 at the groove opening 202. This is beneficial for the user to speed up the protein immunoblotting experiment when operating the device, and also facilitates the user's observation of the reagent tank 101 during the experiment and makes timely adjustments.

[0025] In this embodiment, the experimenter places the protein, antigen, and gel into the corresponding reagent tank 101, and places the reagent tank 101 into the slot 202 located in the disk 208 on the housing 1, thereby making contact with the housing 203. The experimenter starts the housing 1, and the boom 102 begins to rotate on the housing 1. At this time, the boom 102 mixes the raw materials in different reagent tanks 101 for protein immunoblotting experiments. When the experiment requires shaking, the experimenter controls the motor 205 to start rotating. The motor 205 drives the gear 206 and the internal gear 207 to rotate. At this time, the two gears above the internal gear 207... The two tracks 209 rotate coaxially. The circumferences of the two tracks 209 are different, and the rising sections 210 on the two tracks 209 are staggered. This causes the protein sample in the reagent tank 101 to exert a certain gravitational force on the reagent tank 101, compressing the V-shaped elastic sheet 213 between the reagent tank 101 and the receiving shell 203. The receiving shell 203 moves downward within the internal groove 212. The two guide rods 204 below the receiving shell 203 simultaneously contact their corresponding tracks 209. The two guide rods 204 are of identical length and have a circular bottom. At this time, the guide rods 204 are in contact with the tracks 209. Due to gravity, the receiving shell 203 moves downward. The lifting sections 210 of the two tracks 209 are misaligned. Although the lower end of the guide rod 204 is in contact with the track 209, the receiving shell 203 is in an inclined state. When the two tracks 209 rotate, the track 209 presses the guide rod 204, causing the receiving shell 203 to reciprocate and reverse its tilting motion within the built-in groove 212. This causes the receiving shell 203 to drive the reagent tank 101 to make a left-right rocking motion, which can shake the protein sample in the reagent tank 101, facilitating the acceleration of the protein immunoblotting experiment. When the protein immunoblotting experiment reaches the point where shaking is no longer needed, the experimenter can... Pulling the circular block 219 into the built-in groove 214 causes the housing 203 to separate the guide rod 204 from the track 209, thus manually stopping the shaking of the individual reagent tank 101. By rotating the circular block 219, the rotating rod 216 squeezes the circular rod 217 into the circular groove 218, fixing the slider 215 in the built-in groove 212. This fixes the housing 203 in the built-in groove 212, which allows the experimenter to adjust the shaking of the reagent tank 101 according to the progress of the experiment, thereby speeding up the protein immunoblotting experiment and improving the accuracy of the experimental results.

[0026] The above specific embodiments are merely several optional embodiments of this utility model. Based on the technical solution of this utility model and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A protein immunoblotting instrument, comprising a housing (1) and a reagent tank (101) for loading reagents, wherein a boom (102) for extending into the reagent tank (101) to perform experiments is mounted on the housing (1), characterized in that: The housing (1) is provided with a shaking mechanism (2) for shaking the reagent tank (101); The shaking mechanism (2) includes a tray (201) installed on the housing (1). The tray (201) has multiple slots (202) continuously opened inside. Each slot (202) is slidably connected to a receiving shell (203) for accommodating a reagent tank (101). The lower end of the receiving shell (203) is symmetrically fixedly connected to two guide rods (204). A motor (205) is fixedly connected inside the housing (1). One end of the output shaft of the motor (205) is fixedly connected to a gear (206). One side of the gear (206) is meshed with an internal gear (207). The upper end of the internal gear (207) is fixedly connected to a disc (208). The disc (208) is rotatably connected to the housing (1), and the upper end of the disc (208) is fixedly connected to two annular tracks (209) that press against the guide rods (204).

2. The protein immunoblotting instrument according to claim 1, characterized in that, The track (209) includes an ascending section (210) and a descending section (211), and the track (209) is a closed-loop wave shape, with the ascending sections (210) on the two tracks (209) being staggered.

3. The protein immunoblotting instrument according to claim 1, characterized in that, Each slot (202) has an internal groove (212) inside. Two sets of V-shaped elastic sheets (213) are symmetrically fixedly connected in the internal groove (212) and respectively fixedly connected to both ends of the receiving shell (203). The tray (201) has multiple internal grooves (214). A slider (215) that slides in the internal groove (214) is fixedly connected to one side of the receiving shell (203). A rotating rod (216) is rotatably connected in the slider (215), and two round rods (217) are symmetrically slidably connected in the slider (215).

4. The protein immunoblotting instrument according to claim 3, characterized in that, The built-in groove 2 (214) is provided with a circular groove (218) for sliding connection with the circular rod (217).

5. A protein immunoblotting instrument according to claim 3, characterized in that, One end of the rotating rod (216) passes through the slider (215). The rotating rod (216) is elliptical in shape, and a round block (219) is fixedly connected to one end of the rotating rod (216).

6. A protein immunoblotting instrument according to claim 4, characterized in that, Both ends of the two round rods (217) are tapered, and the round rods (217) are magnets. The slider (215) is made of plastic, and the rotating rod (216) is made of iron. The round rods (217) and the rotating rod (216) are magnetically connected.

7. A protein immunoblotting instrument according to claim 2, characterized in that, The length of the slot (202) is less than the length of the housing (203).

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