Low-temperature preservation box for strain culture

Abstract

The utility model discloses a strain culture low-temperature preservation box which comprises a box body, at least four culture cavities and at least four assembling cavities are formed in the box body, the four culture cavities are evenly distributed in the box body, the assembling cavities are matched with the culture cavities in number, refrigeration mechanisms are assembled in the assembling cavities, fixing frames are fixedly assembled in the culture cavities, and the fixing frames are fixedly assembled in the box body. A condensate water discharging mechanism is assembled in the box body, and opening and closing doors corresponding to the culture cavities in number are hinged to the side wall of the box body. The interior of the box body is arranged in a partitioned mode, each culture cavity is closed through a corresponding opening and closing door, each culture cavity is subjected to independent temperature control through a refrigeration mechanism, and the opening and closing door of the corresponding culture cavity can be independently opened according to the taking requirement in the strain culture process. Due to the split type layout, the temperature environment in other culture cavities without opening and closing doors is more stable.

Description

Technical Field

[0001] This utility model relates to the field of preservation box technology, specifically to a low-temperature preservation box for bacterial culture. Background Technology

[0002] Microbial culture preservation chambers are laboratory equipment specifically designed for the cultivation and long-term preservation of microorganisms (such as bacteria and fungi). They are widely used in the medical and pharmaceutical industries, maintaining the activity of microorganisms and preventing contamination through precise control of temperature, humidity, and gas environment. While existing microbial culture preservation chambers have independent temperature-controlled zones, most adopt a monolithic layout. Removing microorganisms can cause temperature changes in other zones, and the time required for the temperature in those zones to return to the set temperature can lead to fluctuations in the environment of the cultured microorganisms, affecting the stability of the culture environment. Therefore, we propose a low-temperature preservation chamber for microbial culture. Utility Model Content

[0003] The purpose of this invention is to provide a low-temperature preservation box for bacterial culture to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a low-temperature preservation box for bacterial culture, comprising a box body, wherein a culture chamber and an assembly chamber are provided inside the box body, the number of culture chambers is at least four, the four culture chambers are evenly distributed inside the box body, the number of assembly chambers matches the number of culture chambers, a refrigeration mechanism is installed in the assembly chamber, a fixing frame is fixedly installed in the culture chamber, a condensate drainage mechanism is installed inside the box body, and a hinged door corresponding to the number of culture chambers is hinged to the side wall of the box body;

[0005] The refrigeration mechanism includes a refrigeration body, which is assembled in an assembly cavity. A heat exchanger is fixedly assembled on the rear side wall of the housing. A coolant circulation chamber is opened in the heat exchanger. A connecting pipe is fixedly connected between the input end and the output end of the coolant circulation chamber and the input end and the output end of the refrigeration body, respectively. A circulation pump is fixedly assembled between the connecting pipe and the heat exchanger. A refrigerator is fixedly assembled on the side wall of the heat exchanger.

[0006] Preferably, there are two cooling elements, which are arranged in an arc shape and symmetrically distributed on the left and right sides with the culture chamber as the center. The opposite sidewalls of the two cooling elements are integrally formed with cooling blocks, which are snapped into the inner sidewall of the culture chamber.

[0007] Preferably, the condensate discharge mechanism includes a collection tank, which is located at the bottom of the assembly cavity. The bottom of the culture cavity is provided with collection holes evenly distributed. The inner cavity of the box body is provided with a collection chamber. A connecting cavity is fixedly connected between the collection chamber and the collection tank. A liquid suction pump is fixedly mounted on the rear side wall of the box body. The input end of the liquid suction pump is connected to the inner cavity of the collection chamber.

[0008] Preferably, the top of the fixing frame is provided with a placement slot, and the front end of the fixing frame is fixedly equipped with a label buckle.

[0009] Preferably, an observation window is embedded in the side wall of the door.

[0010] Compared with the prior art, the beneficial effects of this utility model are as follows: A low-temperature preservation box for bacterial culture, compared with traditional preservation boxes, adopts a partitioned layout inside the box. Each culture chamber is sealed by a corresponding door, and each culture chamber is independently temperature-controlled by a refrigeration mechanism. During the bacterial culture process, the door of the corresponding culture chamber can be opened individually as needed. The split layout makes the temperature environment in the other culture chambers with closed doors more stable. At the same time, this utility model has redesigned the refrigeration mechanism and the condensate drainage mechanism. The use of a ring-shaped distribution of refrigeration elements can improve the uniformity of temperature control in the culture chamber, which is beneficial to the control of the temperature of the bacterial culture environment. Meanwhile, the condensate drainage mechanism can also facilitate the collection of condensate generated by the refrigeration mechanism during the refrigeration process, avoiding the retention of condensate in the culture chamber, which would affect the stability of the culture environment. Attached Figure Description

[0011] Figure 1 This is a perspective view of the present invention.

[0012] Figure 2 This is a schematic diagram of the structure of this utility model.

[0013] Figure 3 This is a schematic diagram of the refrigeration mechanism of this utility model.

[0014] Figure 4 This is a schematic diagram of the coolant circulation chamber of this utility model.

[0015] Figure 5 This is a structural schematic diagram of the fixing frame of this utility model.

[0016] Figure 6 This is a rear-view perspective view of the present invention.

[0017] In the diagram: 1. Box body; 2. Culture chamber; 3. Assembly chamber; 4. Refrigeration mechanism; 41. Refrigeration element; 42. Refrigeration block; 43. Heat exchanger; 44. Coolant circulation chamber; 45. Connecting pipe; 46. Circulation pump; 47. Refrigerator; 48. Controller; 5. Opening and closing door; 51. Observation window; 6. Fixing frame; 61. Placement slot; 62. Label clip; 7. Condensate drainage mechanism; 71. Collection tank; 72. Collection hole; 73. Collection chamber; 74. Connecting chamber; 75. Suction pump. Detailed Implementation

[0018] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention.

[0019] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 This utility model provides a technical solution: a low-temperature preservation box for bacterial culture, comprising a box body 1, with culture chambers 2 for low-temperature preservation and culture of bacterial strains within the box body 1. The number of culture chambers 2 is at least four sets, evenly distributed within the box body 1. The box body 1 also has assembly chambers 3, the number of which matches the number of culture chambers 2. Each assembly chamber 3 is connected to its corresponding culture chamber 2, and a refrigeration mechanism 4 is installed between the assembly chamber 3 and its corresponding culture chamber 2. By dividing the box body 1 into zones for the culture chambers 2 and installing a separate refrigeration mechanism 4 in each culture chamber 2, independent refrigeration operation can be performed on each culture chamber 2, employing zoned temperature control. This method allows the same strain to be cultured at different temperatures, or to be cultured in different culture chambers 2 at corresponding temperatures according to the different temperature requirements of the strains. The front side wall of the box 1 is hinged with a switch door 5 for sealing the culture chamber 2. The number of switch doors 5 matches the number of culture chambers 2. By sealing the corresponding culture chamber 2 through the switch doors 5, the independence between each culture chamber 2 can be maximized. Compared with traditional storage boxes, this method avoids the temperature change inside the storage box caused by opening and closing the box door when taking out the strains. The time it takes for the temperature to recover will affect the strains located in the storage box.

[0020] like Figure 2 and Figure 3As shown, the refrigeration mechanism 4 includes two refrigeration bodies 41, which are arc-shaped and symmetrically fixed in the assembly cavity 3. The opposite sidewalls of the two refrigeration bodies 41 are integrally formed with uniformly distributed refrigeration blocks 42, which are snapped into the sidewalls of the culture cavity 2. A heat exchanger 43 is fixedly installed on the rear sidewall of the box 1. The heat exchanger 43, refrigeration bodies 41 and refrigeration blocks 42 are all made of thermally conductive metal materials. The inner cavity of the heat exchanger 43 has a spirally distributed coolant circulation cavity 44. The input and output ends of the coolant circulation cavity 44 are connected to the input and output ends of the refrigeration body 41 through connecting pipes 45, respectively. A circulation pump 46 is also installed between the connecting pipe 45 and the heat exchanger 43. The circulation pump 46 is electrically connected to an external power source. A cooler 47 is fixedly installed on the sidewall of the heat exchanger 43. The inner cavities of the heat exchanger 43 and the refrigeration body 41 are filled with coolant.

[0021] The coolant circulates fully within the cooler 41, allowing it to cool the environment inside the culture chamber 2. Simultaneously, the circulation pump 46 drives the coolant to circulate between the cooler 41 and the heat exchanger 43. After its temperature rises in the cooler 41, the coolant enters the heat exchanger 43 and is cooled through the spirally distributed coolant circulation chamber 44 within the heat exchanger 43. The cooler 47 further cools the heat exchanger 43. The cooler 47 is electrically connected to an external power supply and a controller 48, which is fixedly mounted on the side wall of the switch door 5. The controller 48 allows input of the required temperature parameters, enabling temperature settings within the culture chamber 2. A temperature sensor detects the temperature data, which is then transmitted to a controller 48. The controller 48 adjusts the cooling effect of the cooler 47 according to the set temperature, thereby maintaining the stability of the ambient temperature inside the culture chamber 2 (this is a mature application of existing technology, therefore the installation of the temperature sensor is not shown in the accompanying drawings). The difference between this invention and existing cooling solutions is that an arc-shaped cooling body 41, symmetrically distributed on the left and right, cools the culture chamber 2 from both sides in a ring shape, which can improve the uniformity of the ambient temperature inside the culture chamber 2 and improve the stability of the culture environment.

[0022] like Figure 2 and Figure 5 As shown, a fixing frame 6 is fixedly installed inside the culture chamber 2. The fixing frame 6 is welded and fixed to the inner side wall of the culture chamber 2. There are at least two fixing frames 6 in each culture chamber 2. The two fixing frames 6 are evenly distributed from top to bottom in the culture chamber 2. The front end of the fixing frame 6 is provided with a placement groove 61. The placement groove 61 is used to place the culture dish for the culture strain. The front side wall of the fixing frame 6 is welded and fixed with a label clip 62. The label clip 62 is used for inserting a label. The label is used to record the information of the strain placed in the placement groove 61.

[0023] like Figure 1 As shown, an observation window 51 is embedded in the front side wall of the switch door 5. The observation window 51 is made of tempered glass. By setting the observation window 51, it is convenient to observe the label information in the label buckle 62 at the front end of the fixing frame 6 and to distinguish the bacterial species cultured in the culture chamber 2.

[0024] like Figure 2 and Figure 6 As shown, a condensate drainage mechanism 7 is provided inside the housing 1. The condensate drainage mechanism 7 can discharge the condensate generated in the culture chamber 2 in a timely manner. The condensate drainage mechanism 7 includes a collection tank 71, which is opened at the bottom of the assembly chamber 3. The bottom of the culture chamber 2 is evenly provided with collection holes 72 that communicate with the assembly chamber 3. The bottom of the inner cavity of the housing 1 is provided with a collection chamber 73. The inner cavity of the housing 1 is provided with a connecting cavity 74. One end of the connecting cavity 74 is connected to the bottom of the collection tank 71, and the other end of the connecting cavity 74 is connected to the inner cavity of the collection chamber 73. The connecting cavity 74 is inclined. The condensate in the culture chamber 2 slides down its inner cavity sidewall to the bottom and enters the collection tank 71 through the collection holes 72. At the same time, the condensate in the assembly chamber 3 also slides down its inner cavity sidewall into the collection tank 71. The condensate enters the collection chamber 73 through the connecting cavity 74 for centralized collection.

[0025] A liquid suction pump 75 is fixedly installed on the rear side wall of the housing 1. The input end of the liquid suction pump 75 is connected to the inner cavity of the collection chamber 73, and the output end of the liquid suction pump 75 is connected to the external waste liquid discharge pipe. The liquid suction pump 75 is electrically connected to an external power supply and an external controller. The external controller can be a timer switch. The liquid suction pump 75 is turned on at a timer to discharge the condensate in the collection chamber 73 to the outside, so as to avoid the accumulation of condensate in the collection chamber 73. The opening time of the liquid suction pump 75 is set to once every 12 hours or 24 hours to avoid frequent opening of the liquid suction pump 75, which would cause air flow in the inner cavity of each culture chamber 2 and affect the temperature stability of each culture chamber 2.

[0026] Working principle: During use, the microbial strains to be preserved and cultured are placed into the fixed rack 6 in the corresponding culture chamber 2. A label recording the microbial information is inserted into the label clip 62 at the front end of the fixed rack 6. The ambient temperature in the corresponding culture chamber 2 can be set according to the required culture temperature of the microbial strains. The refrigeration mechanism 4 can cool the culture chamber 2 according to the set temperature. When it is necessary to remove the microbial strains from the box 1, the switch door 5 of the corresponding culture chamber 2 can be opened separately to avoid affecting the temperature environment of other areas of the culture chamber 2 during the process of removing the microbial strains. During the cultivation of microbial strains in the box 1, the condensate drainage mechanism 7 can be opened at regular intervals to discharge the condensate generated in the culture chamber 2 and the assembly chamber 3, preventing the condensate from accumulating in the culture chamber 2 and the assembly chamber 3 and affecting the stability of the internal environment of the culture chamber 2.

Claims

1. A bacteria culture cryopreservation box comprising a box body (1), characterized in that: The box (1) is provided with culture cavities (2) and assembly cavities (3), the number of the culture cavities (2) is at least four, the four culture cavities (2) are uniformly distributed in the box (1), the number of the assembly cavities (3) matches that of the culture cavities (2), the assembly cavities (3) are provided with refrigeration mechanisms (4), the culture cavities (2) are fixedly provided with fixing frames (6), the box (1) is provided with condensate water discharge mechanisms (7), and the side wall of the box (1) is hingedly provided with switch doors (5) corresponding to the culture cavities (2). The refrigeration mechanism (4) comprises a refrigeration body (41), the refrigeration body (41) is assembled in the assembly cavity (3), the rear side wall of the box (1) is fixedly provided with a heat exchange body (43), the heat exchange body (43) is provided with a cooling liquid circulating cavity (44), the input end and the output end of the cooling liquid circulating cavity (44) are fixedly connected with the input end and the output end of the refrigeration body (41) through connecting pipes (45), and the connecting pipes (45) are fixedly provided with circulating pumps (46) between the connecting pipes (45) and the heat exchange body (43).

2. The bacterial strain culture low-temperature preservation box according to claim 1, characterized in that: The number of the refrigeration body (41) is two, the two refrigeration bodies (41) are symmetrically distributed around the culture cavities (2), and the opposite side walls of the two refrigeration bodies (41) are integrally provided with refrigeration blocks (42), the refrigeration blocks (42) are clamped on the inner cavity side walls of the culture cavities (2).

3. The bacterial strain culture low-temperature preservation box according to claim 1, characterized in that: The condensate water discharge mechanism (7) comprises a collecting groove (71), the collecting groove (71) is arranged at the bottom of the assembly cavity (3), the bottom of the culture cavity (2) is uniformly provided with a collecting hole (72), the inner cavity of the box (1) is provided with a collecting cavity (73), the collecting cavity (73) and the collecting groove (71) are fixedly connected through a connecting cavity (74), and the rear side wall of the box (1) is fixedly provided with a liquid suction pump (75).

4. The bacterial strain culture low-temperature preservation box according to claim 1, characterized in that: The top of the fixing frame (6) is provided with a placing groove (61), and the front end of the fixing frame (6) is fixedly provided with a label buckle (62).

5. The bacterial strain culture low-temperature preservation box according to claim 1, characterized in that: The side wall of the switch door (5) is embeddedly provided with an observation window (51).

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