Fluid infusion device for cell culture based on fusion filtering technology

Abstract

The utility model relates to the technical field of cell culture, and discloses a fluid infusion device for cell culture based on a fusion filtering technology, which comprises a base, a support seat is fixed above the base, and one side of the support seat is movably connected with a mounting plate. The first motor is started to drive the first stirring rod and the second stirring rod to rotate so as to start stirring work, meanwhile, the first stirring rod and the second stirring rod are designed to be distributed in a crossed mode, the stirring effect of the stirring assembly can be effectively improved, meanwhile, a filter screen is installed, a culture solution can be further filtered, and the stirring efficiency is improved. The filter screen is simple in structure, the purity of the filter screen is guaranteed, the filtering work is more efficient under the mutual cooperation of the slow flow channels, meanwhile, reinforcing ribs are installed in the slow flow channels, the strength of the filter screen can be enhanced, the filter screen is prevented from being damaged under the impact of a large amount of culture solution for a long time, the service life of the filter screen is prolonged, and the use value of the filter screen is increased.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a fluid replenishment device for cell culture based on fusion filtration technology. Background Technology

[0002] Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cellular synthesis and metabolism, cell growth and proliferation can be studied. During the cell culture process in the incubator, the cells will consume the culture medium in the incubator, and the staff needs to replenish the culture medium in the incubator regularly.

[0003] The aforementioned existing technical solutions have the following shortcomings:

[0004] Firstly, existing cell culture replenishment devices have high requirements for the quality of the culture medium. However, when the culture medium is stored for a long time, sediment is prone to occur inside the storage tank, which reduces its quality and makes it difficult to complete subsequent cell culture work smoothly.

[0005] Secondly, existing cell culture replenishment devices usually provide quantitative replenishment, but the amount of culture medium consumed and required varies for each type of cell during the culture process, making it difficult to replenish the corresponding amount of culture medium according to the actual situation.

[0006] Therefore, we need to design a cell culture fluid replenishment device based on fusion filtration technology to solve the problems mentioned above. Utility Model Content

[0007] The purpose of this invention is to provide a cell culture replenishment device based on fusion filtration technology, in order to solve the problem mentioned in the background art that the culture medium inside the existing cell culture replenishment device is prone to precipitation after long-term use.

[0008] To achieve the above objectives, this utility model provides the following technical solution: a cell culture fluid replenishment device based on fusion filtration technology, comprising a base, a support seat fixed above the base, and an installation plate movably connected to one side of the support seat. A first motor is fixed above the installation plate, a carrier seat is fixed inside the support seat below the installation plate, and a culture medium storage tank is fixed inside the carrier seat. A limiting groove is provided above the base, and a culture dish is fixed inside the limiting groove. A stirring assembly is provided below the first motor. An outlet pipe is fixed below the culture medium storage tank, and a control valve is fixed outside the outlet pipe. A sliding groove is provided inside the support seat.

[0009] Preferably, the stirring assembly includes a rotating shaft connected to the output end of a first motor. A stirring seat is fixed to the outer side of the rotating shaft. The culture medium storage tank has an internal cavity into which the stirring assembly is inserted. A first stirring rod is fixed to the inner side of the stirring seat, and a second stirring rod is fixed to the outer side of the rotating shaft. The two are distributed in a crisscross pattern. A dual-head motor is fixed below the rotating shaft, and a connecting seat is fixed inside the dual-head motor. A connecting shaft is rotatably connected to the output ends on both sides of the connecting seat, and stirring blades are fixed at the upper and lower ends of the connecting shaft.

[0010] Preferably, the stirring blades are inclined and are arranged at equal intervals on the outside of the connecting shaft.

[0011] Preferably, a slow-flow channel is provided below the receiving cavity, and a filter screen is fixed below the slow-flow channel, with reinforcing ribs distributed inside the filter screen.

[0012] Preferably, a limiting plate is fixed inside the limiting groove, and a second motor is fixed inside the limiting plate. A positive and negative threaded screw is rotatably connected to the output end of the second motor, and a threaded sleeve is fitted on the outer side of the positive and negative threaded screw. A clamping seat is fixed above the threaded sleeve, and a V-shaped groove is opened on the inner side of the clamping seat, which can clamp culture dishes of different sizes. A weighing sensor is fixed below the limiting plate.

[0013] Preferably, a threaded rod is fixed at the front end of the base, and a microcontroller is fixed on the outside of the threaded rod. The output end of the weighing sensor is electrically connected to the input end of the microcontroller through a wire, and the output end of the microcontroller is electrically connected to the input end of the control valve through a wire.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] (1) By starting the first motor, the first stirring rod and the second stirring rod are driven to rotate, so as to start the stirring work. At the same time, the first stirring rod and the second stirring rod are designed to be distributed in a cross shape, which can effectively improve the stirring effect of the stirring components. At the same time, by starting the double-head motor to drive the stirring blades on both sides to rotate, the phenomenon of sedimentation of the culture medium during long-term storage in the culture medium storage tank can be prevented, thus ensuring the quality of the culture medium and promoting the efficient and smooth progress of subsequent work. At the same time, by installing a filter screen, the culture medium can be further filtered to ensure its purity. With the cooperation of the slow flow channel, the filtration work is made more efficient. At the same time, by installing reinforcing ribs inside the slow flow channel, the strength of the filter screen can be strengthened, preventing damage to the filter screen under long-term impact of a large amount of culture medium, thus extending the service life and value of the filter screen.

[0016] (2) By starting the second motor and under the action of the threaded connection, the clamping seat can be driven to move closer to the culture dish, thereby limiting the culture dish. At the same time, by installing a weighing sensor, when the culture dish and its internal culture medium reach the preset value of the weighing sensor, it will transmit the signal to the microcontroller. The microcontroller receives the signal, processes it and transmits it to the control valve. At this time, the control valve can be closed to stop the culture medium supply. The corresponding supply can be given according to the amount of culture medium required by different cultured cells. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall three-dimensional structure of this utility model;

[0019] Figure 2 This is a front view cross-sectional structural diagram of the culture medium storage tank of this utility model;

[0020] Figure 3 This is a side sectional view of the base structure of this utility model;

[0021] Figure 4 This is a three-dimensional structural diagram of the filter screen of this utility model;

[0022] Figure 5 This is a schematic diagram of the circuit structure of this utility model.

[0023] The following are the annotations in the diagram: 1. Support base; 2. Slide groove; 3. First motor; 4. Mounting plate; 5. Culture medium storage tank; 6. Bearing base; 7. Base; 8. Discharge pipe; 9. Threaded rod; 10. Microcontroller; 11. Limiting groove; 12. Culture dish; 13. Receiving cavity; 14. Rotating shaft; 15. First stirring rod; 16. Second stirring rod; 17. Stirring base; 18. Connecting base; 19. Dual-head motor; 20. Slow flow channel; 21. Filter screen; 22. Stirring blade; 23. Connecting shaft; 24. Clamping base; 25. Limiting plate; 26. Second motor; 27. Threaded sleeve; 28. Weighing sensor; 29. ​​Positive and negative threaded screw; 30. Reinforcing rib. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.

[0025] Example 1

[0026] To address the issue of sedimentation in the culture medium of existing cell culture replenishment devices after prolonged use, the following solution is disclosed, as detailed below. Figure 1 , Figure 2 and Figure 4 As shown:

[0027] A cell culture fluid replenishment device based on fusion filtration technology includes a base 7, a support base 1 fixed above the base 7, and an mounting plate 4 movably connected to one side of the support base 1. A first motor 3 is fixed above the mounting plate 4. A carrier 6 is fixed inside the support base 1 below the mounting plate 4, and a culture medium storage tank 5 is fixed inside the carrier 6. A limiting groove 11 is provided above the base 7, and a culture dish 12 is fixed inside the limiting groove 11. A stirring assembly is provided below the first motor 3. An outlet pipe 8 is fixed below the culture medium storage tank 5, and a control valve is fixed outside the outlet pipe 8. A sliding groove 2 is provided inside the support base 1.

[0028] The stirring assembly includes a rotating shaft 14, which is connected to the output end of the first motor 3. A stirring seat 17 is fixed on the outside of the rotating shaft 14. The culture medium storage tank 5 has an internal cavity 13, into which the stirring assembly is inserted. A first stirring rod 15 is fixed on the inside of the stirring seat 17, and a second stirring rod 16 is fixed on the outside of the rotating shaft 14. The two are distributed in a cross pattern. A double-headed motor 19 is fixed below the rotating shaft 14, and a connecting seat 18 is fixed inside the double-headed motor 19. A connecting shaft 23 is rotatably connected to the output ends on both sides of the connecting seat 18, and stirring blades 22 are fixed at the upper and lower ends of the connecting shaft 23.

[0029] The stirring blades 22 are inclined and are evenly spaced on the outside of the connecting shaft 23;

[0030] A slow-flow channel 20 is provided below the receiving cavity 13, and a filter screen 21 is fixed below the slow-flow channel 20. Reinforcing ribs 30 are distributed inside the filter screen 21.

[0031] In this embodiment, when in use, the first motor 3 is started to drive the first stirring rod 15 and the second stirring rod 16 to rotate, so as to start the stirring work. At the same time, the dual-head motor 19 is started to drive the stirring blades 22 on both sides to rotate, which can prevent the culture medium from precipitating when stored in the culture medium storage tank 5 for a long time, thereby ensuring the quality of the culture medium. After the culture medium is stirred and fused, it can be further filtered through the slow flow channel 20 and then through the filter screen 21 to ensure its purity.

[0032] Example 2

[0033] This embodiment differs from Embodiment 1 in that, by using the weighing sensor 28, the appropriate amount of culture medium can be supplied according to the actual situation. Specifically, as shown below... Figure 3 and Figure 5 As shown:

[0034] A limiting plate 25 is fixed inside the limiting groove 11, and a second motor 26 is fixed inside the limiting plate 25. A positive and negative thread screw 29 is rotatably connected to the output end of the second motor 26, and a threaded sleeve 27 is sleeved on the outside of the positive and negative thread screw 29. A clamping seat 24 is fixed above the threaded sleeve 27, and a V-shaped groove is opened on the inner side of the clamping seat 24, which can clamp culture dishes 12 of different sizes. A weighing sensor 28 is fixed below the limiting plate 25.

[0035] A threaded rod 9 is fixed at the front end of the base 7, and a microcontroller 10 is fixed on the outside of the threaded rod 9. The output end of the load cell 28 is electrically connected to the input end of the microcontroller 10 through a wire, and the output end of the microcontroller 10 is electrically connected to the input end of the control valve through a wire.

[0036] In this embodiment, during the supply of culture medium, when the weighing sensor 28 senses the weight and reaches the preset value, it transmits a signal to the microcontroller 10. The microcontroller 10 receives the signal, processes it, and transmits it to the control valve. At this time, the control valve can be closed to stop the supply of culture medium. Thus, the supply can be adjusted according to the amount of culture medium required by different cultured cells.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for the replenishment of cell culture based on the fusion filtration technique, comprising a base (7), characterised in that: The upper side of the base (7) is fixedly connected with a supporting seat (1), and the side of the supporting seat (1) is movably connected with a mounting plate (4), and the upper side of the mounting plate (4) is fixedly connected with a first motor (3), and the inner side of the supporting seat (1) below the mounting plate (4) is fixedly connected with a bearing seat (6), and the inside of the bearing seat (6) is fixedly connected with a culture solution storage tank (5), and the upper side of the base (7) is provided with a limiting groove (11), and the inside of the limiting groove (11) is fixedly connected with a culture dish (12), and the lower side of the first motor (3) is provided with a stirring assembly, and the lower side of the culture solution storage tank (5) is fixedly connected with a liquid outlet pipe (8), and the outer side of the liquid outlet pipe (8) is fixedly connected with a control valve, and the inner side of the supporting seat (1) is provided with a sliding groove (2).

2. The device according to claim 1, wherein the device is a device for replenishing a cell culture medium based on a fusion filtration technique. The stirring assembly comprises a rotating shaft (14) which is connected with the output end of the first motor (3), and the outer side of the rotating shaft (14) is fixedly connected with a stirring seat (17), and the inside of the culture solution storage tank (5) is provided with a containing cavity (13), and the stirring assembly is inserted into the containing cavity (13), and the inner side of the stirring seat (17) is fixedly connected with a first stirring rod (15), and the outer side of the rotating shaft (14) is fixedly connected with a second stirring rod (16), and the two are cross-distributed, and the lower side of the rotating shaft (14) is fixedly connected with a double-head motor (19), and the inside of the double-head motor (19) is fixedly connected with a connecting seat (18), and the output ends of the two sides of the connecting seat (18) are rotatably connected with a connecting shaft (23), and the upper and lower ends of the connecting shaft (23) are fixedly connected with stirring blades (22).

3. The device according to claim 2, wherein the device is a device for replenishing a cell culture medium based on a fusion filtration technique. The shape of the stirring blades (22) is arranged in an inclined shape, and the outer side of the connecting shaft (23) is arranged in an equidistant distribution.

4. The device according to claim 2, wherein the device is characterized by: The lower side of the containing cavity (13) is provided with a slow-flow channel (20), and the lower side of the slow-flow channel (20) is fixedly connected with a filter screen (21), and the inside of the filter screen (21) is distributed with reinforcing ribs (30).

5. The device according to claim 1, wherein the device is characterized by: The inside of the limiting groove (11) is fixedly connected with a limiting plate (25), and the inside of the limiting plate (25) is fixedly connected with a second motor (26), and the output end of the second motor (26) is rotatably connected with a forward and reverse toothed screw (29), and the outer side of the forward and reverse toothed screw (29) is sleeved with a threaded sleeve (27), and the upper side of the threaded sleeve (27) is fixedly connected with a clamping seat (24), and the inner side of the clamping seat (24) is provided with a V-shaped groove, which can clamp culture dishes (12) of different sizes, and the lower side of the limiting plate (25) is fixedly connected with a weighing sensor (28).

6. The device according to claim 5, wherein the device is a device for replenishing a cell culture medium based on a fusion filtration technique. The front end of the base (7) is fixedly connected with a threaded rod (9), and the outer side of the threaded rod (9) is fixedly connected with a single-chip microcomputer (10), and the output end of the weighing sensor (28) is electrically connected with the input end of the single-chip microcomputer (10) through wires, and the output end of the single-chip microcomputer (10) is electrically connected with the input end of the control valve through wires.

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