Cell scratch culture plate
By designing a cell scratch culture plate, using positioning lines and limiting grooves to adjust the position of the scratch inserts, and combining it with biocompatible silicone material, the problems of scratch inconsistency and contamination in existing devices have been solved, achieving cell experiments with high accuracy and convenience.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2026-03-06
AI Technical Summary
Existing cell scratching devices cannot guarantee the consistency and accuracy of scratches, are prone to cell damage and contamination, and are cumbersome and inconvenient to operate.
A cell scratch culture plate was designed, comprising a cell culture plate, positioning lines, limiting grooves, and scratch inserts made of biocompatible silicone material. The positioning lines accurately identify and position the inserts, the limiting grooves adjust the position of the inserts, and the silicone material fixes the inserts to avoid cell damage.
It improves the accuracy and reproducibility of experiments, reduces cell damage and contamination, simplifies experimental preparation, and enhances operational convenience.
Smart Images
Figure CN223974115U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cell experimental device technology, specifically relating to cell scratch culture plates. Background Technology
[0002] In cell biology research, understanding cell migration capabilities is crucial for studying many physiological and pathological processes. For example, epithelial cell migration plays a key role in wound healing; the migration capabilities of tumor cells are essential for understanding and testing tumor metastasis. Cell scratch culture plates were developed to meet the needs of cell migration research.
[0003] Traditional cell scratching methods have several drawbacks, such as difficulty in ensuring the consistency and accuracy of scratches, the potential for additional cell damage during the scratching process, and the possible introduction of impurities and contamination that can affect experimental results. Furthermore, existing scratching tools and methods need improvement in terms of ease of operation and experimental efficacy. Some experimental setups are complex, cumbersome, and bulky, hindering operator operation and limiting experimental flexibility and scalability. Other setups using plug-in modules have fixed sizes and shapes, limiting the area and number of cells that can be cultured, making them unsuitable for experiments requiring large numbers of cells or specific cell densities. Therefore, a cell culture plate that allows for convenient and accurate cell scratching while meeting experimental requirements is needed. Utility Model Content
[0004] The purpose of this invention is to provide a cell scratch culture plate that solves the problems of existing cell scratch devices, which are difficult to guarantee the consistency and accuracy of scratches and are prone to contamination.
[0005] The technical solution adopted by this utility model is: a cell scratch culture plate, including a cell culture plate, a number of culture tanks on the cell culture plate, a number of positioning lines on the back of the cell culture plate at the corresponding position of each culture tank, a number of limiting grooves on the outer periphery of the culture tank, and scratch inserts inserted into the culture tank.
[0006] The technical solution adopted in this utility model is also characterized by:
[0007] Furthermore, the scratch-resistant insert adjusts its insertion position via a limiting groove.
[0008] Furthermore, the bottom of the scratch-making insert is bonded to the bottom of the culture tank when creating scratches.
[0009] Furthermore, the scratch-resistant insert has a square handle.
[0010] Furthermore, the scratch-resistant insert is inserted into the culture tank to conform to the curve of the culture tank.
[0011] Furthermore, the limiting groove is set at a position where several scratch-resistant inserts can be fixed vertically and parallel.
[0012] Furthermore, the positioning lines are equidistant and parallel, and do not exceed the size of the culture tank.
[0013] Furthermore, the cell culture plate is equipped with a removable lid on top.
[0014] The beneficial effects of this utility model are:
[0015] The cell scratch culture plate of this invention, by setting positioning lines, allows experimenters to accurately identify and locate the cells during operation, thereby improving the accuracy and reproducibility of the experiment.
[0016] The cell scratch culture plate of this invention can form a blank area without residue to meet experimental requirements by setting a scratch insert. After culturing cells in the culture tank, the insert is lowered to achieve the purpose of creating scratches.
[0017] This novel cell scratch culture plate uses biocompatible silicone material in the portion of the insert that contacts the bottom and sidewalls of the culture tank. It is bonded to the main body via adhesive. The silicone material has excellent adhesion, securing the insert firmly while ensuring that cell removal is not damaged and leaves no residue.
[0018] The cell scratch culture plate of this invention has a limiting groove that allows for adjustment of the insertion position of the insert.
[0019] The cell scratch culture plate of this invention comes pre-installed with scratch inserts, reducing pre-experiment preparation and improving the convenience of experimental operation. Attached Figure Description
[0020] Figure 1 This is a schematic diagram of the positioning line structure of the cell scratch culture plate of this utility model;
[0021] Figure 2 This is a schematic diagram of the cell scratch culture plate culture tank structure of this utility model;
[0022] Figure 3 This is a schematic diagram of the limiting groove and scratch insert structure of the cell scratch culture plate of this utility model;
[0023] Figure 4 This is a schematic diagram of the structure of the cell scratch culture plate box cover of this utility model;
[0024] Figure 5 This is a schematic diagram of the scratch insertion structure of the cell scratch culture structure of this utility model.
[0025] In the diagram: 1. Cell culture plate, 2. Box lid, 3. Culture tank, 4. Limiting groove, 5. Scratch insert, 6. Positioning line. Detailed Implementation
[0026] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.
[0027] This utility model provides a cell scratch culture plate, including a cell culture plate 1, a cover 2, and several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1, which are parallel and equidistant from each other and do not exceed the size of the culture tank 3. A limiting groove 4 is provided on the culture tank 3. The upper limiting groove 4 of the culture tank 3 is positioned to vertically and parallelly fix several scratch inserts 5. Scratch inserts 5 are inserted into the culture tank 3. The scratch inserts 5 can be lowered into the bottom of the culture tank 3 through the limiting groove 4 to create scratches.
[0028] When creating scratches, the bottom of the scratch insert 5 is bonded to the bottom of the culture tank 3.
[0029] The scratch-resistant insert 5 is inserted into the culture tank 3 and partially conforms to the curve of the culture tank 3.
[0030] This novel cell scratch culture plate, with its positioning lines, allows for easy identification and positioning by researchers during operation, improving the accuracy and reproducibility of experiments. The inclusion of scratch inserts creates a residue-free blank area to meet experimental requirements. After cells are cultured in the culture tank, the inserts are lowered to create scratches. The portion of the insert that contacts the bottom and sidewalls of the culture tank is made of biocompatible silicone material and is bonded to the main body via adhesive. The silicone material has excellent adhesion, securing the insert without damaging cells or leaving residue when the insert is removed. A limiting groove allows for adjustment of the insert's lowering position. The cell culture plate comes pre-installed with the scratch inserts, reducing pre-experiment preparation and improving operational convenience.
[0031] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.
[0032] Example 1
[0033] Cell scratch culture plates, such as Figure 1 , Figure 2 and Figure 4As shown, the device includes a cell culture plate 1 with a lid 2 and several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1, which facilitate accurate identification and positioning by the experimenter during operation, improving the accuracy and repeatability of the experiment. Limiting grooves 4 are provided on the culture tanks 3, and scratching inserts 5 are inserted into the culture tanks 3. By setting the scratching inserts, a blank area without residue can be formed to meet experimental requirements. After culturing cells in the culture tanks, the inserts are lowered in to achieve the purpose of creating scratches.
[0034] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4. By setting the limiting groove, the insertion position of the insert can be adjusted. The scratch-resistant insert 5, as shown... Figure 5 As shown.
[0035] When creating the scratches, the bottom of the scratching insert 5 is bonded to the bottom of the culture tank 3. The part of the insert that contacts the bottom and sidewalls of the culture tank is made of biocompatible silicone material and is bonded to the main body by adhesive. The silicone material has good adhesion, which can fix the insert in place, and at the same time, it will not damage the cells and leave no residue when the insert is removed.
[0036] Example 2
[0037] Cell scratch culture plates, such as Figure 1 , Figure 2 and Figure 4 As shown, the device includes a cell culture plate 1 with a lid 2 and several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1, which facilitate accurate identification and positioning by the experimenter during operation, improving the accuracy and repeatability of the experiment. Limiting grooves 4 are provided on the culture tanks 3, and scratching inserts 5 are inserted into the culture tanks 3. By setting the scratching inserts, a blank area without residue can be formed to meet experimental requirements. After culturing cells in the culture tanks, the inserts are lowered in to achieve the purpose of creating scratches.
[0038] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4. By setting the limiting groove, the insertion position of the insert can be adjusted.
[0039] When creating the scratches, the bottom of the scratching insert 5 is bonded to the bottom of the culture tank 3. The part of the insert that contacts the bottom and sidewalls of the culture tank is made of biocompatible silicone material and is bonded to the main body by adhesive. The silicone material has good adhesion, which can fix the insert in place, and at the same time, it will not damage the cells and leave no residue when the insert is removed.
[0040] Scratch plug 5 has a square handle.
[0041] In this embodiment, as Figure 2As shown, the cell culture plate 1 has four rows and six columns of cell culture tanks 3. The middle two rows, namely the second and third rows of culture tanks 3, are inserted with scratching inserts 5 for use. The surrounding two rows, namely the first and fourth rows of culture tanks 3, are not inserted with scratching inserts 5. They are placed there after the scratching inserts 5 in the second and third rows have been used to create scratches.
[0042] Example 3
[0043] Cell scratch culture plates, such as Figure 1 , Figure 2 and Figure 4 As shown, the device includes a cell culture plate 1 with a lid 2 and several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1, which facilitate accurate identification and positioning by the experimenter during operation, improving the accuracy and repeatability of the experiment. Limiting grooves 4 are provided on the culture tanks 3, and scratching inserts 5 are inserted into the culture tanks 3. By setting the scratching inserts, a blank area without residue can be formed to meet experimental requirements. After culturing cells in the culture tanks, the inserts are lowered in to achieve the purpose of creating scratches.
[0044] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4. By setting the limiting groove, the insertion position of the insert can be adjusted.
[0045] When creating the scratches, the bottom of the scratching insert 5 is adhered to the bottom of the culture tank 3. The insertion portion of the scratching insert 5 conforms to the curve of the culture tank 3. The part of the insert that contacts the bottom and sidewall of the culture tank is made of biocompatible silicone material and is bonded to the main body by adhesive. The silicone material has good adhesion, which can fix the insert in place, and at the same time, it will not damage the cells and leave no residue when the insert is removed.
[0046] Scratch plug 5 has a square handle.
[0047] In this embodiment, as Figure 3 As shown, each culture tank 3 can be fitted with three fixed scratch inserts 5, and three scratches can be created in each culture tank 3, which is suitable for some experiments that require a large number of cells or a specific cell density.
[0048] Example 4
[0049] Cell scratch culture plates, such as Figure 1 , Figure 2 and Figure 4As shown, the device includes a cell culture plate 1 with a lid 2 and several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1, which facilitate accurate identification and positioning by the experimenter during operation, improving the accuracy and repeatability of the experiment. Limiting grooves 4 are provided on the culture tanks 3, and scratching inserts 5 are inserted into the culture tanks 3. By setting the scratching inserts, a blank area without residue can be formed to meet experimental requirements. After culturing cells in the culture tanks, the inserts are lowered in to achieve the purpose of creating scratches.
[0050] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4. By setting the limiting groove, the insertion position of the insert can be adjusted.
[0051] When creating the scratches, the bottom of the scratching insert 5 is adhered to the bottom of the culture tank 3. The insertion portion of the scratching insert 5 conforms to the curve of the culture tank 3. The part of the insert that contacts the bottom and sidewall of the culture tank is made of biocompatible silicone material and is bonded to the main body by adhesive. The silicone material has good adhesion, which can fix the insert in place, and at the same time, it will not damage the cells and leave no residue when the insert is removed.
[0052] Scratch plug 5 has a square handle.
[0053] The limiting groove 4 is set at a position where several scratch-resistant inserts 5 can be fixed vertically and parallel.
[0054] In this embodiment, the cell culture plate 1 is provided with four rows and six columns of cell culture tanks 3. Each culture tank 3 is provided with six limiting grooves 4. Two scratch inserts 5 can be inserted and fixed into each culture tank 3. The back of the cell culture plate 1 has five equidistant parallel positioning lines that do not exceed the size of the culture tank 3. This allows the experimenter to accurately identify and position the tank during operation, thereby improving the accuracy and repeatability of the experiment.
[0055] In this embodiment, the cell culture plate 1 comes with a pre-fixed scratch insert 5, which can be used directly for culturing and inoculation, and then the scratch insert 5 can be removed to create blank marks, reducing the preparation work before the experiment and improving the convenience of the experimental operation.
[0056] Example 5
[0057] The cell scratch culture plate includes a cell culture plate 1, a number of culture tanks 3 on the cell culture plate 1, a number of positioning lines 6 on the back of the cell culture plate 1 at the corresponding position of each culture tank 3, a limit groove 4 on the outer periphery of the culture tank 3, and a scratch insert 5 inserted into the culture tank 3.
[0058] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4.
[0059] When creating scratches, the bottom of the scratch insert 5 is bonded to the bottom of the culture tank 3.
[0060] Scratch plug 5 has a square handle.
[0061] The scratch-resistant insert 5 is inserted into the culture tank 3 and partially conforms to the curve of the culture tank 3.
[0062] The limiting groove 4 is set at a position where several scratch-resistant inserts 5 can be fixed vertically and parallel.
[0063] The positioning lines 6 are equidistant and parallel, and do not exceed the size of the culture tank 3.
[0064] The scratching insert 5 achieves the purpose of creating scratches by adjusting its position in the limiting groove 4. Before creating scratches, the scratching insert 5 is located at the high position in the limiting groove, and there is a certain height difference between the bottom of the scratching insert 5 and the culture tank 3 for cell culture work. When scratches need to be created, the scratching insert 5 is located at the low position in the limiting groove 4, and the bottom of the scratching insert 5 is in contact with the culture tank 3 to create scratches.
[0065] Example 6
[0066] The cell scratch culture plate includes a cell culture plate 1, which has several culture tanks 3. Several positioning lines 6 are provided on the back of the cell culture plate 1 at corresponding positions in each culture tank. Several limiting grooves 4 are provided on the outer periphery of the culture tanks 3. Scratch inserts 5 are inserted into the culture tanks 3.
[0067] The scratch-resistant insert 5 adjusts its insertion position via the limiting groove 4.
[0068] When creating scratches, the bottom of the scratch insert 5 is bonded to the bottom of the culture tank 3.
[0069] Scratch plug 5 has a square handle.
[0070] The scratch-resistant insert 5 is inserted into the culture tank 3 and partially conforms to the curve of the culture tank 3.
[0071] The limiting groove 4 is set at a position where several scratch-resistant inserts 5 can be fixed vertically and parallel.
[0072] The positioning lines 6 are equidistant and parallel, and do not exceed the size of the culture tank 3.
[0073] The cell culture plate 1 is equipped with a removable lid 2 on top.
[0074] The method of using the cell scratch culture plate of this invention is as follows:
[0075] After culturing cells in culture tank 3, the scratching insert 5 is lowered in to achieve the purpose of creating scratches.
[0076] After the cells have been cultured for a certain period of time, use tweezers to remove the scratch insert 5 that is fixed in the culture tank 3 through the limiting groove 4. After processing, put the lid 2 back on and use the positioning line 6 on the back of the cell culture plate 1 to locate and observe the culture area and the blank area.
[0077] This novel cell scratch culture plate, by setting several positioning lines 6, allows experimenters to accurately identify and position the cells during operation, thereby improving the accuracy and reproducibility of the experiment.
[0078] By setting the scratching plug-in 5, a blank area without residue can be formed to meet experimental requirements. After culturing cells in the cell culture plate 1, the scratching plug-in 5 is lowered in to achieve the purpose of creating scratches.
[0079] The cell culture plate 1 is equipped with several culture tanks 3. Experimenters can adjust the number of culture tanks 3 as needed to meet different experimental requirements.
[0080] The part of the scratching insert 5 that contacts the bottom and side wall of the culture tank 3 is made of biocompatible silicone material. It is bonded to the main body by adhesive. The silicone material has good adhesion and can fix the insert. At the same time, it will not damage the cells and leave no residue when the scratching insert 5 is removed.
[0081] The insertion position of the plug-in can be adjusted by setting the limit slot 4; the number of limit slots 4 can be adjusted according to needs to meet different experimental requirements.
[0082] The cell scratch culture plate of this invention comes with a pre-fixed scratch insert 5 at the factory, which reduces the preparation work before the experiment and improves the convenience of the experimental operation.
Claims
1. A cell scratch culture plate, characterized by, The utility model provides a cell culture plate, which comprises a cell culture plate (1) provided with a plurality of culture grooves (3), a plurality of positioning lines (6) arranged on the back of the cell culture plate (1) at positions corresponding to the culture grooves (3), a plurality of limiting grooves (4) arranged on the outer periphery of the culture grooves (3), and scratch inserts (5) inserted into the culture grooves (3).
2. The cell scratch culture plate of claim 1, wherein, The scratch inserts (5) are adjusted in the insertion position through the limiting grooves (4).
3. The cell scratch culture plate of claim 1, wherein, When the scratch inserts (5) make scratches, the bottom part is bonded to the bottom part of the culture grooves (3).
4. The cell scratch culture plate of claim 1, wherein, The scratch inserts (5) are square handle parts.
5. The cell scratch culture plate of claim 1, wherein, The scratch inserts (5) are inserted into the culture grooves (3) and partially adhere to the curve of the culture grooves (3).
6. The cell scratch culture plate of claim 1, wherein, The limiting grooves (4) are arranged at positions at which a plurality of scratch inserts (5) can be vertically and parallelly fixed.
7. The cell scratch culture plate of claim 1, wherein, The positioning lines (6) are equidistantly parallel and do not exceed the size of the culture grooves (3).
8. The cell scratch culture plate of claim 6, wherein, The cell culture plate (1) is provided with a detachable box cover (2) on the top.