Polypeptide synthesis equipment

Abstract

The utility model relates to the technical field of polypeptide synthesis, and particularly discloses polypeptide synthesis equipment. The device comprises a tank body, the motor is arranged at the top of the tank body; the driving synchronous pulley is mounted at the output end of the motor; the driven synchronous pulley is in transmission connection with the driving synchronous pulley; the connecting shaft is coaxially connected with the driven synchronous pulley; the discharging brush is connected with the bottom of the connecting shaft; the discharging brush is located in the tank body, and a screen is arranged below the discharging brush. Through the synergistic effect of the screen and the rotating brush, materials are depolymerized under the dual effects of centrifugal force and shearing force, small-particle reactants can enter reaction liquid through the screen, and uniform dispersion of particles in the reaction liquid is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of peptide synthesis technology, specifically to a peptide synthesis device. Background Technology

[0002] In the solid-phase synthesis of peptides, when reactants (such as resin carriers) come into contact with the reaction liquid, they are prone to agglomeration (clumping phenomenon) due to surface tension, which leads to a sharp reduction in the reaction interface and a decrease in mass transfer efficiency, directly affecting the coupling reaction rate and product purity.

[0003] Utility model application CN202420285924.X discloses a polypeptide synthesis device, belonging to the field of polypeptide synthesis technology. It addresses the problem that reactants easily clump together when in contact with the reaction liquid, preventing sufficient reaction and reducing the quality of the synthesized polypeptide. The device includes a tank with an annular tube at the top. The inner wall of the annular tube has several circumferentially distributed discharge holes, which are inclined. An inner cover is fixed to one side of the top surface of the annular tube, and a connecting rod is mounted on the top surface of the inner cover. A lever plate is fixed to the end of the connecting rod, and the lever plate is movably engaged within the annular tube. A limit sleeve is fixed to the top of the inner wall of the tank. This utility model, through the annular tube, discharge holes, and lever plate, allows the reactants to fall in a scattered manner onto the surface of the reaction liquid, avoiding the clumping phenomenon that prevents sufficient reaction and reduces the quality of the synthesized polypeptide.

[0004] However, the patent has a complex structure and uses planetary gears, which are costly.

[0005] Therefore, it is necessary to provide a new technical solution to overcome the above-mentioned defects. Utility Model Content

[0006] The purpose of this invention is to provide a polypeptide synthesis device that can effectively solve the above-mentioned technical problems.

[0007] To achieve the purpose of this utility model, the following technical solution is adopted:

[0008] A polypeptide synthesis apparatus, comprising: a tank;

[0009] The motor is located at the top of the tank;

[0010] A drive synchronous pulley installed at the output end of the motor;

[0011] The driven synchronous pulley is connected to the driving synchronous pulley.

[0012] The connecting shaft is coaxially connected to the driven synchronous pulley;

[0013] A feeding brush connected to the bottom of the connecting shaft;

[0014] The feeding brush is located inside the tank, and a screen is provided below the feeding brush.

[0015] Furthermore, a discharge chamber is also installed on the top of the tank, and the discharge chamber is coaxially connected to the connecting shaft;

[0016] An auger is also installed on the connecting shaft, and the auger is located inside the feeding chamber.

[0017] Furthermore, the output end of the motor is also coaxially connected to a stirring shaft;

[0018] The stirring shaft is provided with a first stirring blade and a second stirring blade from top to bottom, with the first stirring blade located below the screen.

[0019] Furthermore, the tank body is rotatably mounted on the control box.

[0020] Furthermore, a control panel is installed on the control box.

[0021] Compared with the prior art, this application has the following beneficial effects: Through the synergistic effect of the screen and the rotating brush, the material is deagglomerated under the dual action of centrifugal force and shear force, so that small particulate reactants can pass through the screen and enter the reaction liquid, which helps the particles to be evenly dispersed in the reaction liquid. Attached Figure Description

[0022] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0023] Figure 1 This is a schematic diagram of the structure of a polypeptide synthesis device according to this invention;

[0024] Figure 2 This is a schematic diagram of a polypeptide synthesis device according to the present invention;

[0025] Figure 3 This is a schematic diagram of the internal structure of a polypeptide synthesis device according to this invention;

[0026] Figure 4 This is a schematic diagram of the internal structure of the feeding chamber of a polypeptide synthesis device according to this invention.

[0027] In the diagram: 1. Control box; 2. Tank body; 3. Motor; 4. Motor mounting base; 5. Driving synchronous pulley; 6. Driven synchronous pulley; 7. Synchronous belt; 8. Connecting shaft; 9. Discharge chamber; 10. Screwdriver; 11. Discharge brush; 12. Screen; 13. First stirring blade; 14. Second stirring blade; 15. Stirring shaft; 16. Discharge box. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions in this utility model will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this utility model, but not all embodiments.

[0029] In the description of this utility model, it should be understood that the terms "center," "lateral," "longitudinal," "front," "rear," "left," "right," "upper," "lower," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this utility model. When a component is referred to as being "fixed to" another component, it can be directly on the other component or there may be an intermediate component. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be an intermediate component at the same time. When a component is considered to be "set on" another component, it can be directly set on the other component or there may be an intermediate component at the same time. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only.

[0030] like Figures 1 to 4 As shown, this utility model discloses a polypeptide synthesis device, comprising: a control box 1, a tank 2 rotatably mounted on the control box 1, and a feeding box 16 placed below the discharge end of the tank 2; the control box 1 includes: boxes symmetrically arranged on both sides of the tank 2, a power source installed inside one of the boxes, and a transmission shaft coaxially connected to the output end of the power source, the transmission shaft being connected to the middle of the tank 2 so that the power source can drive the tank 2 to swing; correspondingly, a rotating shaft is connected to the middle of the other side of the tank 2, the rotating shaft being rotatably connected to the other box; the swinging of the tank 2 can cause the reactants and reaction liquid to continuously tumble and flow within the tank 2, thereby breaking up the agglomeration between materials, increasing the contact area between the reactants and reaction liquid, and improving mass transfer efficiency.

[0031] A control panel is installed on one of the boxes of the control box 1. An angle sensor can be added at the drive shaft or rotating shaft. The angle sensor is electrically connected to the control panel and is used to monitor the swing angle of the tank 2 in real time.

[0032] Optionally, a double-layer jacket structure is added to the outer wall of the tank body 2, and circulating water is introduced into the jacket. The circulating water can be circulating hot water or circulating cold water. The temperature of the medium is adjusted by an external temperature control device to indirectly heat the reaction liquid in the tank or to cool the reaction liquid in the heated tank. The external temperature control device can be a cylindrical electric heater, a refrigerator, etc. Temperature sensors are installed inside the tank body 2 at the inlet and outlet of the double-layer jacket. The temperature sensors are electrically connected to the control panel for real-time monitoring of temperature distribution. The target temperature can be set by manually operating the control panel.

[0033] The top of the tank body 2 is provided with a motor mounting base 4, and the motor 3 is mounted on the motor mounting base 4; the output end of the motor 3 is provided with a driving synchronous pulley 5; the driving synchronous pulley 5 is drivenly connected to the driven synchronous pulley 6; the driven synchronous pulley 6 is coaxially connected to the connecting shaft 8; the bottom of the connecting shaft 8 is connected to the feeding brush 11; the feeding brush 11 is located inside the tank body 2, and a screen 12 is provided below the feeding brush 11; a connecting rod is connected to the side of the screen 12, and the other end of the connecting rod is connected to the inside of the tank body 2; the feeding brush 11 rotates under the drive of the motor 3, which can rub the reactant on the screen 12 into small particles of reactant; wherein, the feeding brush 11 can be set as a ring, and the middle part is connected to the connecting shaft 8 through the connecting rod, so that the material can fall from the middle of the feeding brush 11.

[0034] In solid-phase peptide synthesis, reactants such as resin carriers are prone to agglomeration due to surface tension. The rotation of the feed brush 11 can break this agglomeration, allowing the material to fall into the reaction solution in the form of small particles. This increases the contact area between the reactants and the reaction solution, avoids the formation of agglomerates when the materials come into contact with the reaction solution, and ensures that the reaction solution can fully react with the reactants.

[0035] The top of the tank body 2 is also equipped with the discharge chamber 9, which is coaxially connected to the connecting shaft 8. The connecting shaft 8 is also equipped with the auger 10, which is located inside the discharge chamber 9. The rotation of the auger 10 can continuously stir the material in the discharge chamber 9, preventing the material from accumulating and clumping in the discharge chamber 9 and causing blockage. By adjusting the ratio of the driving pulley 5 to the driven pulley 6, the speed and amount of material falling can be precisely controlled when the motor 3 drives the stirring, ensuring that the reactants enter the tank body 2 stably and evenly. The driving pulley 5, the driven pulley 6, and the synchronous belt 7 can also be selected as a gear chain structure.

[0036] The output end of the motor 3 is also coaxially connected to the stirring shaft 15; the stirring shaft 15 is provided with the first stirring blade 13 and the second stirring blade 14 from top to bottom. The first stirring blade 13 is located below the screen 12. During the rotation of the stirring blade, it can fully stir the reactants and reaction liquid in the tank 2, so that the two are mixed more evenly. The first stirring blade 13 works below the screen 12, which can quickly mix the small particles of reactants falling through the screen 12 with the reaction liquid. The second stirring blade 14 can stir the reaction liquid in the lower part of the tank 2 to prevent the reaction liquid from stratifying and ensure the uniformity of the reaction environment in the entire tank 2.

[0037] Working principle: When using this device, the operator first sets the stirring temperature, speed and other parameters through the control panel, and then starts the motor 3. The output end of the motor 3 drives the stirring shaft to rotate, which in turn drives the first stirring blade 13 and the second stirring blade 14 to stir the material in the tank 2. At the same time, the output end of the motor 3 also drives the active synchronous pulley 5 to rotate, which in turn drives the driven synchronous pulley 6 to rotate. When the driven synchronous pulley 6 rotates, on the one hand, it drives the auger 10 to stir the material in the feeding chamber 9, and on the other hand, the driven synchronous pulley 6 drives the feeding brush 11 to rub the reaction material on the screen 12 into small particles.

[0038] All standard parts used in this utility model can be purchased from the market. Irregular parts can be customized according to the description in the instruction manual and the attached drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the existing technology. The machinery, parts and equipment adopt conventional models in the existing technology. In addition, the circuit connection adopts conventional connection methods in the existing technology, which will not be described in detail here. The contents not described in detail in this instruction manual belong to the prior art known to those skilled in the art.

[0039] It should be understood that those skilled in the art can make improvements or modifications based on the above description, and all such improvements and modifications should fall within the protection scope of the appended claims.

Claims

1. A polypeptide synthesis apparatus, characterized by, Include: Tank body; Motor arranged at the top of the tank body; Driving synchronous pulley mounted on the output end of the motor; Driven synchronous pulley in driving connection with the driving synchronous pulley; The connecting shaft is coaxially connected with the driven synchronous pulley; The blanking brush is connected with the bottom of the connecting shaft; The blanking brush is located inside the tank body, and a screen is arranged below the blanking brush.

2. A polypeptide synthesis apparatus as claimed in claim 1, characterized in that The top of the tank body is also provided with a discharging chamber, which is coaxially connected with the connecting shaft; The connecting shaft is also provided with an auger, which is located inside the discharging chamber.

3. The polypeptide synthesis apparatus of claim 1, wherein The output end of the motor is also coaxially connected with the stirring shaft; The first stirring blade and the second stirring blade are arranged on the stirring shaft from top to bottom in sequence, and the first stirring blade is located below the screen.

4. The polypeptide synthesis apparatus of claim 1, wherein The tank body is rotatably installed on the control box.

5. A polypeptide synthesis apparatus as claimed in claim 4, wherein The control panel is installed on the control box.

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