Cryopreservation filling system

Abstract

The utility model relates to the technical field of cryopreservation equipment, in particular to a cryopreservation filling system. Comprising a cryopreservation bag, a first catheter and a second catheter, and the cryopreservation bag is communicated with the first catheter and the second catheter; one end, far away from the cryopreservation bag, of the first guide pipe is connected with a sample inlet pipe through a first reducing adapter, and the other end of the second guide pipe is connected with a sample outlet pipe through a second reducing adapter; the first reducing adapter is composed of a first connector and a second connector in a clamping mode, the second reducing adapter is composed of a first connector and a second connector in a clamping mode, the first guide pipe, the second guide pipe and the anti-freezing bag are all made of anti-freezing materials including but not limited to C-Flex materials or EVA materials, and the sample inlet pipe and the sample outlet pipe are both made of PVC materials; meanwhile, the second guide pipe and the sample outlet pipe are connected in a clamping mode through the first connector and the second connector, and the problems that an existing cryopreservation bag is prone to frost crack and pollution are better solved.

Description

Technical Field

[0001] This utility model relates to the field of cryopreservation equipment technology, and in particular to a cryopreservation filling system. Background Technology

[0002] Cryopreservation is one of the main methods for preserving lentiviruses and cells. Currently, the most common storage methods are -196°C liquid nitrogen freezing or -80°C freezing. These methods can almost completely stop the metabolic activities of cells and viruses, thereby maintaining the activity and viability of cells and viruses.

[0003] Before cryopreservation, cells or viruses need to be filled into cryopreservation bags before cryopreservation. During the filling process, the cryopreservation bags need to be connected to the feed bag via tubing. After filling, the tubing on the cryopreservation bags needs to be frozen together with the bags containing the cells or viruses. However, the current antifreeze effect of the tubing connected to the cryopreservation bags is poor, which will affect subsequent use.

[0004] In addition, when filling cells before cryopreservation, the tubing on the cryopreservation bag is often mismatched with the tubing on the feed bag, or when cryopreservation cells are needed, the tubing at the cell location is mismatched with the tubing on the cryopreservation bag. This makes it difficult to directly connect the cryopreservation bag to other tubing. If a direct hard connection is made, it is not completely sealed, making it difficult to guarantee aseptic operation throughout the process, which in turn leads to cell contamination.

[0005] Therefore, there is an urgent need to provide a cryopreservation filling system that can better solve the problems of existing cryopreservation bags being prone to freezing and cracking and contamination compared to existing technologies. Utility Model Content

[0006] This invention addresses the technical problems existing in the prior art and provides a cryopreservation and filling system.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0008] A cryopreservation filling system includes a cryopreservation bag, a first conduit, and a second conduit, wherein the cryopreservation bag is connected to the first conduit and the second conduit.

[0009] The end of the first conduit away from the cryopreservation bag is connected to the sample inlet tube via a first reducing adapter, and the end of the second conduit away from the cryopreservation bag is connected to the sample outlet tube via a second reducing adapter. The two ends of the first reducing adapter are respectively fitted inside the first conduit and the sample inlet tube, and the two ends of the second reducing adapter are respectively fitted inside the second conduit and the sample outlet tube.

[0010] Furthermore, the first conduit and the second conduit are disposed on the same side or different sides of the cryopreservation bag.

[0011] Furthermore, the first catheter, the second catheter, and the cryopreservation bag are all made of antifreeze material, including but not limited to C-Flex material and EVA material. The inlet tube and the outlet tube are both made of PVC material, and the cryopreservation bag is integrally connected to the first catheter and the second catheter.

[0012] Furthermore, the outer walls of the first conduit and the first variable diameter adapter sleeve joint, the outer walls of the sample inlet tube and the first variable diameter adapter sleeve joint, the outer walls of the second conduit and the second variable diameter adapter sleeve joint, and the outer walls of the sample outlet tube and the second variable diameter adapter sleeve joint are all provided with third cable ties for fixing the first variable diameter adapter and the second variable diameter adapter.

[0013] Furthermore, the second reducing adapter consists of a first connector and a second connector. The first connector is connected to the second conduit, and the second connector is connected to the sample outlet tube. The side wall of the first connector is provided with a first snap-fit ​​groove and a first snap-fit ​​post. The side wall of the second connector is connected with a second snap-fit ​​groove and a second snap-fit ​​post. The first snap-fit ​​post extends into the second snap-fit ​​groove and snaps into the second snap-fit ​​groove. The second snap-fit ​​post extends into the first snap-fit ​​groove and snaps into the first snap-fit ​​groove.

[0014] Furthermore, a first cable tie is fitted over the outer wall of the connection between the second catheter and the first connector, and a second cable tie is fitted over the outer wall of the connection between the sample outlet tube and the second connector.

[0015] Furthermore, the second conduit is provided with a flow-stopping clamp at the end near the cryopreservation bag.

[0016] Furthermore, a flow-stopping clamp is provided at the end of the first conduit near the cryopreservation bag.

[0017] Furthermore, after filling is completed, the end of the first conduit near the cryopreservation bag is heat-sealed with the cryopreservation bag to seal the cryopreservation filling system.

[0018] Furthermore, the end of the sample inlet tube away from the first conduit is provided with a first plug, and the end of the sample outlet tube away from the second conduit is provided with a second plug.

[0019] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0020] (1) The first conduit, the second conduit and the cryopreservation bag are made of C-Flex or EVA material, which has good anti-freezing effect, and when the cell therapy material is filled in the cryopreservation bag for cryopreservation and transportation, the first conduit and the second conduit can be cryopreserved together with the cryopreservation bag, and good cryopreservation effect is achieved.

[0021] (2) The first connecting head and the second connecting head are used for clamping connection between the second conduit and the sample outlet pipe, and the first cable tie and the second cable tie are used for clamping the connection between the second conduit and the first connecting head and the connection between the sample outlet pipe and the second connecting head, so that good sealing and sterility are achieved. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 is a schematic diagram of the overall structure of the embodiment 1 of the utility model.

[0023] Figure 2 is a schematic diagram of the overall structure of the embodiment 2 of the utility model.

[0024] Figure 3 is a schematic diagram of the structure of the adapter in the embodiment 2 of the utility model.

[0025] BRIEF DESCRIPTION OF DRAWINGS

[0026] 1, cryopreservation bag; 2, first conduit; 3, second conduit; 4, first variable diameter adapter; 5, sample inlet pipe; 6, sample outlet pipe; 7, second variable diameter adapter; 71, first connecting head; 711, first clamping column; 712, first clamping groove; 72, second connecting head; 721, second clamping column; 722, second clamping groove; 8, flow stop clamp; 9, first plug; 10, first cable tie; 11, second plug; 12, second cable tie; 13, third cable tie. DETAILED DESCRIPTION

[0027] The technical solutions of the utility model will be clearly described below in combination with the drawings, and obviously, the described embodiments are not all the embodiments of the utility model, and all other embodiments obtained by the ordinary skilled in the art without creative labor belong to the protection scope of the utility model. It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the utility model.

[0028] Embodiment 1

[0029] As Figure 1As shown, the embodiment provides a freeze filling system, which comprises a freeze bag 1, a first conduit 2 and a second conduit 3, the freeze bag 1 is communicated with the first conduit 2 and the second conduit 3, and the freeze bag 1, the first conduit 2 and the second conduit 3 are integrally formed, that is, the freeze bag 1 can be input through the first conduit 2, and the freeze bag 1 can be output through the second conduit 3.

[0030] The freeze bag 1, the first conduit 2 and the second conduit 3 are all made of anti-freezing material, which includes but is not limited to C-Flex material and EVA material, and the freeze bag 1, the first conduit 2 and the second conduit 3 connected with the freeze bag 1 can withstand-90℃ low-temperature preservation, thereby reducing the risk of rupture of the pipeline due to the inability to withstand low-temperature preservation, and ensuring the sterile environment in the process of filling, preservation and use.

[0031] The freeze filling system further comprises a sample inlet pipe 5 and a sample outlet pipe 6, the pipeline of the first conduit 2 and the second conduit 3 is not matched in size with the pipeline of a conventional feed bag and a use place, and the sample inlet pipe 5 and the sample outlet pipe 6 are further provided for docking. The other end of the first conduit 2 is connected with the sample inlet pipe 5 through a first variable-diameter adapter 4, and the other end of the second conduit 3 is connected with the sample outlet pipe 6 through a second variable-diameter adapter 7, the two ends of the first variable-diameter adapter 4 are respectively sleeved in the first conduit 2 and the sample inlet pipe 5, and the two ends of the second variable-diameter adapter 7 are respectively sleeved in the second conduit 3 and the sample outlet pipe 6, when the sample is input and output, the connection of the sample inlet pipe 5 with the feed bag and the connection of the sample outlet pipe 6 with the use place can be directly realized, which is convenient to use and can ensure the sterile operation in the whole process.

[0032] The first conduit 2 and the second conduit 3 can be arranged on the same side or different sides of the freeze bag 1, and are preferably arranged on the same side of the freeze bag 1 to save space, but can also be arranged on different sides of the freeze bag 1, which is not limited by the utility model.

[0033] In order to make the first variable-diameter adapter 4 and the second variable-diameter adapter 7 be firmly connected, the outer wall of the sleeving part of the first conduit 2 and the first variable-diameter adapter 4, the outer wall of the sleeving part of the sample inlet pipe 5 and the first variable-diameter adapter 4, the outer wall of the sleeving part of the second conduit 3 and the second variable-diameter adapter 7, and the outer wall of the sleeving part of the sample outlet pipe 6 and the second variable-diameter adapter 7 are all provided with a third cable tie 13 for fixing the first variable-diameter adapter 4 and the second variable-diameter adapter 7, preventing the first variable-diameter adapter 4 from being separated from the first conduit 2 and the sample inlet pipe 5, and preventing the second variable-diameter adapter 7 from being separated from the second conduit 3 and the sample outlet pipe 6.

[0034] In order to prevent the liquid from flowing back after filling, the first conduit 2 and the second conduit 3 are provided with flow stoppers 8 near one end of the cryopreservation bag 1. When inputting, the flow stopper 8 outside the first conduit 2 is opened, and the flow stopper 8 outside the second conduit 3 is closed, so that the sample enters the cryopreservation bag 1 through the first conduit 2; after filling, the flow stoppers 8 outside the first conduit 2 and the second conduit 3 are both closed, preventing the liquid from flowing out.

[0035] In order to ensure the sterility of the cryopreservation filling system, before starting filling, the first plug 9 is installed at the end of the first conduit 2 away from the sample inlet tube 5, and the second plug 11 is installed at the end of the second conduit 3 away from the sample outlet tube 6; when filling, the first plug 9 at the sample inlet tube 5 is removed, and then the sample inlet tube 5 is aseptically welded with the filling main pipeline, and the cryopreservation bag 1 is filled; after filling, the end of the first conduit 2 near the cryopreservation bag 1 is heat-sealed, which can better preserve and block the contact with the outside world, so that the sample is not contaminated.

[0036] In order to fix the first plug 9 and the second plug 11, the outer wall of the connection between the sample inlet tube 5 and the first plug 9 and the outer wall of the connection between the sample outlet tube 6 and the second plug 11 are both provided with a third cable tie 13, so that the connection between the first plug 9 and the sample inlet tube 5 and the connection between the second plug 11 and the sample outlet tube 6 are more firm.

[0037] The use method of the cryopreservation filling system is as follows:

[0038] When filling, the first plug 9 at the sample inlet tube 5 is removed first, the filling main pipeline is aseptically welded with the sample inlet tube 5, then the flow stopper 8 outside the first conduit 2 is opened, and the sample enters the cryopreservation bag 1 through the sample inlet tube 5 and the first conduit 2; at the same time, the flow stopper 8 outside the second conduit 3 remains closed until the liquid occupies 1%-100% of the cryopreservation bag 1, and the filling is completed.

[0039] After filling, the flow stopper 8 outside the first conduit 2 is closed to prevent the liquid from flowing out, and the end of the first conduit 2 near the cryopreservation bag 1 is heat-sealed. After sealing, the pipeline after the sealing point can be cut off, or the sample inlet tube 5 can be removed, and then the cryopreservation bag 1, the first conduit 2 (part or all), the second conduit 3 and the sample outlet tube 6 are stored together. The heat-sealed sealing of the first conduit 2 and the second plug 11 at the sample outlet tube 6 can block the contact between the cells and the outside world, so that the cells are in a sterile closed environment during the cryopreservation process.

[0040] When the sample needs to be output outward, the sample outlet tube 6 is connected with other pipelines by heat sealing, and the flow stopper 8 outside the second conduit 3 is opened, so that the sample flows out through the second conduit 3 and the sample outlet tube 6.

[0041] Example 2

[0042] As Figure 2As shown, the embodiment provides a freezing filling system, which comprises a freezing bag 1, a liquid inlet assembly and a liquid outlet assembly, the freezing bag 1 is provided with a liquid inlet and a liquid outlet, the liquid inlet assembly is connected at the liquid inlet of the freezing bag 1, and the liquid outlet assembly is connected at the liquid outlet of the freezing bag 1; the liquid inlet assembly and the liquid outlet assembly can be arranged at the same side or different sides of the freezing bag 1, and the freezing bag 1 is made of EVA material.

[0043] The liquid inlet assembly comprises a first conduit 2, a first variable-diameter adapter 4 and a sample inlet tube 5, one end of the first conduit 2 is connected at the liquid inlet of the freezing bag 1, the other end of the first conduit 2 is connected to the first variable-diameter adapter 4, the first variable-diameter adapter 4 is connected to the sample inlet tube 5 away from the one end of the first conduit 2, and the end of the sample inlet tube 5 away from the first variable-diameter adapter 4 is connected to the first plug 9; the two ends of the first variable-diameter adapter 4 respectively extend into the interiors of the first conduit 2 and the sample inlet tube 5; the first conduit 2 is made of anti-freezing material such as C-Flex or EVA material, and the sample inlet tube 5 is made of PVC material.

[0044] The liquid outlet assembly comprises a second conduit 3, a second variable-diameter adapter 7 and a sample outlet tube 6, the second variable-diameter adapter 7 comprises a first connecting head 71 and a second connecting head 72, the second conduit 3 is connected at the liquid outlet of the freezing bag 1, one end of the second conduit 3 away from the freezing bag 1 is connected to the first connecting head 71, the end of the first connecting head 71 away from the second conduit 3 is connected to the second connecting head 72, the end of the second connecting head 72 away from the first connecting head 71 is connected to the sample outlet tube 6, and the end of the sample outlet tube 6 away from the second connecting head 72 is connected to the second plug 11; the second conduit 3 is provided with a flow stop clamp 8; the end of the first connecting head 71 away from the second connecting head 72 extends into the interior of the second conduit 3, and a first cable tie 10 is sleeved outside the second conduit 3, and the first cable tie 10 is tightened at the connection between the second conduit 3 and the first connecting head 71; the end of the second connecting head 72 away from the first connecting head 71 extends into the sample outlet tube 6, and a second cable tie 12 is sleeved outside the sample outlet tube 6, and the second cable tie 12 is tightened at the connection between the sample outlet tube 6 and the second connecting head 72; the second conduit 3 is made of C-Flex or EVA material, and the sample outlet tube 6 is made of PVC material.

[0045] The flow stop clamp 8 is arranged to prevent liquid backflow after filling is completed, and the flow stop clamp 8 is in a closed state during filling and freezing to prevent liquid from leaking out.

[0046] As Figure 3As shown, the first connector 71 and the second connector 72 are snapped together. Both the first connector 71 and the second connector 72 have internal channels for liquid flow. The first connector 71 has a first snap-fit ​​groove 712 and a first snap-fit ​​post 711 on one side end face. The second connector 72 has a second snap-fit ​​groove 722 and a second snap-fit ​​post 721 on one side end face. The first snap-fit ​​groove 712 and the first snap-fit ​​post 711 are correspondingly arranged, and the first snap-fit ​​post 711 and the second snap-fit ​​groove 722 are correspondingly arranged. After the first snap-fit ​​post 711 extends into the first snap-fit ​​groove 712 and the second snap-fit ​​post 721 extends into the second snap-fit ​​groove 722, the first connector 71 and the second connector 72 are snapped together. A snap-fit ​​connection between the second conduit 3 and the outlet tube is provided with a first connector 71 and a second connector 72. At the same time, a first cable tie 10 is provided between the first connector 71 and the second conduit 3 for tightening, and a second cable tie 12 is provided between the second connector 72 and the outlet tube for tightening. This can ensure aseptic connection and sealing, and reduce the risk of product contamination.

[0047] To ensure the sterility of the cryopreservation filling system, before filling begins, the inlet tube 5 and the outlet tube 6 are fitted with a first plug 9 and a second plug 11, respectively. During filling, the inlet tube 5 is aseptically connected to the main filling pipeline (e.g., aseptic welding, i.e., immediately connecting the pipeline after high-temperature melting to form a sterile interface) and filled into the cryopreservation bag 1. After filling is completed, the end of the first conduit 2 closest to the cryopreservation bag 1 is heat-sealed to better preserve the sample, prevent it from contacting the outside world, and keep the sample from being contaminated.

[0048] The two ends of the first reducing adapter 4, the end of the first connector 71 away from the second connector 72, and the end of the second connector 72 away from the first connector 71 can all be set with different outer diameters, so that pipes of different diameters can be connected. Different specifications of sample inlet tube 5 and sample outlet tube 6 can be selected as needed for convenient connection.

[0049] The method of using the cryopreservation filling system provided by this utility model is as follows:

[0050] During filling, the main filling pipeline is aseptically connected to the sample inlet tube 5 (e.g., aseptic welding, i.e., immediately connecting after the pipeline is melted by high temperature to form a sterile interface). Cell therapy materials are introduced into the cryopreservation bag 1 through the sample inlet tube 5 and the first conduit 2. At the same time, the stop clamp 8 outside the second conduit 3 is kept closed until the liquid occupies 1%-100% of the cryopreservation bag 1, and the filling is then completed.

[0051] After filling, the end of the first catheter 2 near the cryopreservation bag 1 is sealed by heat sealing. After sealing, the tubing after the sealing point can be cut off, or the sample inlet tube 5 can be removed. Then, the cryopreservation bag 1, the first catheter 2 (partially or entirely), the second catheter 3, the first connector 71, the second connector 72, and the second catheter 3 are frozen and transported together. The heat sealing of the first catheter 2 can block the contact between the cells and the outside world, ensuring that the cells are in a sterile and closed environment during the cryopreservation process.

[0052] When external output is required, the sample outlet tube 6 is connected to other pipelines by heat sealing, and the flow stop clamp 8 outside the second conduit 3 is opened, so that the cell therapy material flows out through the second conduit 3 and the sample outlet tube 6.

[0053] This invention uses C-Flex or EVA materials for both the first catheter 2 and the second catheter 3, which have good antifreeze properties. When cell therapy materials are filled into cryopreservation bags 1 for cryopreservation and transportation, the first catheter 2 and the second catheter 3 are frozen together with the cryopreservation bags 1. This not only provides good cryopreservation but also good sealing and sterility, thus better solving the problems of existing cryopreservation bags 1 being prone to freezing and cracking and contamination.

[0054] Finally, it should be noted that the above content is only used to illustrate the technical solution of this utility model, and is not intended to limit the scope of protection of this utility model. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of this utility model do not depart from the essence and scope of the technical solution of this utility model.

Claims

1. A cryopreservation filling system, characterized in that, It includes a cryopreservation bag, a first conduit, and a second conduit, wherein the cryopreservation bag is connected to the first conduit and the second conduit; The end of the first conduit away from the cryopreservation bag is connected to the sample inlet tube via a first reducing adapter, and the end of the second conduit away from the cryopreservation bag is connected to the sample outlet tube via a second reducing adapter. The two ends of the first reducing adapter are respectively fitted inside the first conduit and the sample inlet tube, and the two ends of the second reducing adapter are respectively fitted inside the second conduit and the sample outlet tube.

2. The cryopreservation filling system according to claim 1, characterized in that, The first conduit and the second conduit are located on the same side or different sides of the cryopreservation bag.

3. The cryopreservation filling system according to claim 1, characterized in that, The first catheter, the second catheter, and the cryopreservation bag are all made of antifreeze material, including but not limited to C-Flex material and EVA material. The inlet tube and the outlet tube are both made of PVC material. The cryopreservation bag is integrally connected to the first catheter and the second catheter.

4. The cryopreservation filling system according to claim 1, characterized in that, The outer walls of the first conduit and the first variable diameter adapter, the outer walls of the inlet tube and the first variable diameter adapter, the outer walls of the second conduit and the second variable diameter adapter, and the outer walls of the outlet tube and the second variable diameter adapter are all provided with third cable ties for fixing the first variable diameter adapter and the second variable diameter adapter.

5. The cryopreservation filling system according to claim 1, characterized in that, The second reducing adapter consists of a first connector and a second connector. The first connector is connected to the second conduit, and the second connector is connected to the sample outlet tube. The side wall of the first connector is provided with a first snap-fit ​​groove and a first snap-fit ​​post. The side wall of the second connector is connected with a second snap-fit ​​groove and a second snap-fit ​​post. The first snap-fit ​​post extends into the second snap-fit ​​groove and snaps into the second snap-fit ​​groove. The second snap-fit ​​post extends into the first snap-fit ​​groove and snaps into the first snap-fit ​​groove.

6. The cryopreservation filling system according to claim 5, characterized in that, A first cable tie is fitted over the outer wall of the connection between the second catheter and the first connector, and a second cable tie is fitted over the outer wall of the connection between the sample outlet tube and the second connector.

7. A cryopreservation filling system according to claim 6, characterized in that, The end of the second conduit near the cryopreservation bag is provided with a flow stop clamp.

8. The cryopreservation filling system according to claim 1, characterized in that, The first conduit has a flow stop clamp at the end near the cryopreservation bag.

9. A cryopreservation filling system according to claim 1, characterized in that, After filling is completed, the end of the first conduit near the cryopreservation bag is heat-sealed with the cryopreservation bag to seal the cryopreservation filling system.

10. A cryopreservation filling system according to claim 1, characterized in that, The end of the sample inlet tube away from the first conduit is provided with a first plug, and the end of the sample outlet tube away from the second conduit is provided with a second plug.

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